JPS56102797A - Reagent for measuring enzyme activity participating in purine metabolism - Google Patents
Reagent for measuring enzyme activity participating in purine metabolismInfo
- Publication number
- JPS56102797A JPS56102797A JP506280A JP506280A JPS56102797A JP S56102797 A JPS56102797 A JP S56102797A JP 506280 A JP506280 A JP 506280A JP 506280 A JP506280 A JP 506280A JP S56102797 A JPS56102797 A JP S56102797A
- Authority
- JP
- Japan
- Prior art keywords
- solution
- reagent
- activity
- enzyme activity
- ada
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 3
- 108090000790 Enzymes Proteins 0.000 title abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 2
- 230000004144 purine metabolism Effects 0.000 title 1
- 239000000243 solution Substances 0.000 abstract 9
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 abstract 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 3
- 101710101148 Probable 6-oxopurine nucleoside phosphorylase Proteins 0.000 abstract 3
- 102000030764 Purine-nucleoside phosphorylase Human genes 0.000 abstract 3
- 239000007853 buffer solution Substances 0.000 abstract 3
- 102100036664 Adenosine deaminase Human genes 0.000 abstract 2
- 108010012029 Guanine Deaminase Proteins 0.000 abstract 2
- 102000013587 Guanine deaminase Human genes 0.000 abstract 2
- 108010093894 Xanthine oxidase Proteins 0.000 abstract 2
- 102100033220 Xanthine oxidase Human genes 0.000 abstract 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 abstract 2
- 239000008280 blood Substances 0.000 abstract 2
- 210000004369 blood Anatomy 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- IYKLQEMQEGWXOQ-UHFFFAOYSA-N 3-(7-amino-4-chloro-1-oxoisochromen-3-yl)oxypropyl carbamimidothioate Chemical compound NC1=CC=C2C(Cl)=C(OCCCSC(=N)N)OC(=O)C2=C1 IYKLQEMQEGWXOQ-UHFFFAOYSA-N 0.000 abstract 1
- 101710169336 5'-deoxyadenosine deaminase Proteins 0.000 abstract 1
- OQRXBXNATIHDQO-UHFFFAOYSA-N 6-chloropyridine-3,4-diamine Chemical compound NC1=CN=C(Cl)C=C1N OQRXBXNATIHDQO-UHFFFAOYSA-N 0.000 abstract 1
- 102000055025 Adenosine deaminases Human genes 0.000 abstract 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 abstract 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 abstract 1
- 229930010555 Inosine Natural products 0.000 abstract 1
- 102100036286 Purine nucleoside phosphorylase Human genes 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 229960005305 adenosine Drugs 0.000 abstract 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 1
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 1
- 150000001540 azides Chemical class 0.000 abstract 1
- 238000004040 coloring Methods 0.000 abstract 1
- 229960003786 inosine Drugs 0.000 abstract 1
- 108010009099 nucleoside phosphorylase Proteins 0.000 abstract 1
- 239000011734 sodium Substances 0.000 abstract 1
- 229910052708 sodium Inorganic materials 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To obtain a reagent for measuring purine nucleoside phosphorylase (PNP) activity, adenosine deaminase(ADA) activity, and guanase activity in the blood, in the blood dissolving red corpsucle, and in a culture solution, by combining a substrate solution, coloring solution, enzyme solution, and reaction terminating agent.
CONSTITUTION: For PNP, a substrate solution consists of inosine, sodium azide, and 0.05W2mol buffer solution in pH 7W8, for ADA, it consists of adenosine, sodium, azide, and 0.05W0.2mol buffer solution in pH 7W8, and, for guanase, it consists of guanine, sodium azide, and 0.05W0.2mol buffer solution in pH 6W7. An enzyme solution is a solution of 1.5W4mol xanthine oxidase or xanthine oxidase and prine nucleoside phosphorylase in ammonium sulfate suspension or 40W60wt% glycerol solution. A reaction terminating agent consists of 0.1W0.3mol acitic acid solution, etc.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP506280A JPS6028279B2 (en) | 1980-01-19 | 1980-01-19 | Reagent for measuring enzyme activity involved in purine metabolism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP506280A JPS6028279B2 (en) | 1980-01-19 | 1980-01-19 | Reagent for measuring enzyme activity involved in purine metabolism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS56102797A true JPS56102797A (en) | 1981-08-17 |
| JPS6028279B2 JPS6028279B2 (en) | 1985-07-03 |
Family
ID=11600901
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP506280A Expired JPS6028279B2 (en) | 1980-01-19 | 1980-01-19 | Reagent for measuring enzyme activity involved in purine metabolism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6028279B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60176600A (en) * | 1984-02-24 | 1985-09-10 | Fujimoto Rinshiyou Kensa Kenkyusho:Kk | Method for measuring activity of guanase |
| JPS6117519A (en) * | 1984-07-04 | 1986-01-25 | Maruho Kk | Blood selection for transfusion |
-
1980
- 1980-01-19 JP JP506280A patent/JPS6028279B2/en not_active Expired
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60176600A (en) * | 1984-02-24 | 1985-09-10 | Fujimoto Rinshiyou Kensa Kenkyusho:Kk | Method for measuring activity of guanase |
| JPS6117519A (en) * | 1984-07-04 | 1986-01-25 | Maruho Kk | Blood selection for transfusion |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6028279B2 (en) | 1985-07-03 |
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