JPS54140594A - Enzyme electrode - Google Patents
Enzyme electrodeInfo
- Publication number
- JPS54140594A JPS54140594A JP4887978A JP4887978A JPS54140594A JP S54140594 A JPS54140594 A JP S54140594A JP 4887978 A JP4887978 A JP 4887978A JP 4887978 A JP4887978 A JP 4887978A JP S54140594 A JPS54140594 A JP S54140594A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- nad
- disk
- auxiliary
- oxidized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 8
- 108090000790 Enzymes Proteins 0.000 title abstract 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 2
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 abstract 2
- BAWFJGJZGIEFAR-NNYOXOHSSA-O NAD(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-O 0.000 abstract 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 2
- 229910002804 graphite Inorganic materials 0.000 abstract 2
- 239000010439 graphite Substances 0.000 abstract 2
- 229950006238 nadide Drugs 0.000 abstract 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract 2
- 230000001590 oxidative effect Effects 0.000 abstract 2
- 241000251468 Actinopterygii Species 0.000 abstract 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 abstract 1
- 239000011810 insulating material Substances 0.000 abstract 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 abstract 1
- 235000013372 meat Nutrition 0.000 abstract 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 abstract 1
- 239000000843 powder Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 abstract 1
- UYPYRKYUKCHHIB-UHFFFAOYSA-N trimethylamine N-oxide Chemical compound C[N+](C)(C)[O-] UYPYRKYUKCHHIB-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE: To easily quantitize the (CH3)3N, which is generated in accordance with the putrefaction of fish meat, by fixing special oxidizing and reducing enzyme or an auxiliary enzyme on to in the vicinity of an electric collector, respectively.
CONSTITUTION: As a trimethyl amine oxide reducing enzyme and an auxiliary enzyme, oxidized type nicotinamide adenine dinucleotide NAD is dissolved in a phosphoric acid of pH 7.0. The solution thus prepared is applied to a disk, which is press-molded of graphite powders, and driyed so that it is acted with flutaraldehyde. The graphite disk 4, on the surface of which the enzyme and auxiliary enzyme are bridged, is fixed to a substrate 1 by a screwing member 5, which is made of an insulating material, such that it is in contact with a Pt plate 3 having a lead wire 2 connected therewith. In case there exists (SH3)3N, the catalyzer reaction of the enzymes in the opposite direction to that shown so that the NADH made of NAD+ is oxidized electro-chemically on the disk 4 by the NAD+ and so that the concentration of the (CH3)N can be obtained from the value of the oxidizing current.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP53048879A JPS5816695B2 (en) | 1978-04-24 | 1978-04-24 | enzyme electrode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP53048879A JPS5816695B2 (en) | 1978-04-24 | 1978-04-24 | enzyme electrode |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS54140594A true JPS54140594A (en) | 1979-10-31 |
| JPS5816695B2 JPS5816695B2 (en) | 1983-04-01 |
Family
ID=12815565
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP53048879A Expired JPS5816695B2 (en) | 1978-04-24 | 1978-04-24 | enzyme electrode |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5816695B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5759157A (en) * | 1980-09-27 | 1982-04-09 | Matsushita Electric Ind Co Ltd | Enzyme electrode |
| US4968400A (en) * | 1986-11-20 | 1990-11-06 | Terumo Kabushiki Kaisha | Enzyme sensor |
| US5122456A (en) * | 1987-05-01 | 1992-06-16 | Cambridge Life Science Plc | Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (nadh) in solution |
-
1978
- 1978-04-24 JP JP53048879A patent/JPS5816695B2/en not_active Expired
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5759157A (en) * | 1980-09-27 | 1982-04-09 | Matsushita Electric Ind Co Ltd | Enzyme electrode |
| JPS6243496B2 (en) * | 1980-09-27 | 1987-09-14 | Matsushita Electric Ind Co Ltd | |
| US4968400A (en) * | 1986-11-20 | 1990-11-06 | Terumo Kabushiki Kaisha | Enzyme sensor |
| US5122456A (en) * | 1987-05-01 | 1992-06-16 | Cambridge Life Science Plc | Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (nadh) in solution |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5816695B2 (en) | 1983-04-01 |
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