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JPS54139792A - Electrode - Google Patents

Electrode

Info

Publication number
JPS54139792A
JPS54139792A JP4798478A JP4798478A JPS54139792A JP S54139792 A JPS54139792 A JP S54139792A JP 4798478 A JP4798478 A JP 4798478A JP 4798478 A JP4798478 A JP 4798478A JP S54139792 A JPS54139792 A JP S54139792A
Authority
JP
Japan
Prior art keywords
enzyme
nad
coenzyme
reacted
fix
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP4798478A
Other languages
Japanese (ja)
Other versions
JPS5816693B2 (en
Inventor
Kenichi Nakamura
Shiro Nankai
Takashi Iijima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Panasonic Holdings Corp
Original Assignee
Matsushita Electric Industrial Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Matsushita Electric Industrial Co Ltd filed Critical Matsushita Electric Industrial Co Ltd
Priority to JP53047984A priority Critical patent/JPS5816693B2/en
Publication of JPS54139792A publication Critical patent/JPS54139792A/en
Priority to US06/194,271 priority patent/US4321123A/en
Priority to US06/321,326 priority patent/US4376689A/en
Publication of JPS5816693B2 publication Critical patent/JPS5816693B2/en
Expired legal-status Critical Current

Links

Landscapes

  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To measure the substrate concentrations of enzyme or activity of enzyme rapidly and readily by forming the enzyme electrode through mixing of the coenzyme directly and chemically bound to high molecular carrier and current collectors and using this.
CONSTITUTION: Nicotineamide Adenine Dinucleotide (NAD, coenzyme) is reacted with, e.g., succinic acid anhydride to introduce carboxylic groups and this is reacted with amno hexyl cellulose (high polymer carrier) to fix the NAD on the cellulose through amide binding. The above-mentioned coenzyme 2 having been directly chemically bound 4 to the high polymer carrier 6 is mixed with current collectors (e.g., graphite powder) 3 and this is press-molded to form the NAD fixed electrode, after which it is dipped in a solution containing enzyme (e.g., lactic acid dehydrogenase) 1 to prepare the NAD-enzyme bond, which is then treated with crosslinking reagent (glutaraldenyde, etc.) to fix the enzyme. The substrate concentration measured by using this. If this is used in the state where enzyme is not bound, the measurement of enzyme activity is possible.
COPYRIGHT: (C)1979,JPO&Japio
JP53047984A 1978-04-21 1978-04-21 electrode Expired JPS5816693B2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP53047984A JPS5816693B2 (en) 1978-04-21 1978-04-21 electrode
US06/194,271 US4321123A (en) 1978-04-21 1980-10-06 Coenzyme immobilized electrode
US06/321,326 US4376689A (en) 1978-04-21 1981-11-13 Coenzyme immobilized electrode

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP53047984A JPS5816693B2 (en) 1978-04-21 1978-04-21 electrode

Publications (2)

Publication Number Publication Date
JPS54139792A true JPS54139792A (en) 1979-10-30
JPS5816693B2 JPS5816693B2 (en) 1983-04-01

Family

ID=12790570

Family Applications (1)

Application Number Title Priority Date Filing Date
JP53047984A Expired JPS5816693B2 (en) 1978-04-21 1978-04-21 electrode

Country Status (1)

Country Link
JP (1) JPS5816693B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007240528A (en) * 2006-02-10 2007-09-20 Canon Inc Acquisition device for information as to concentration of thioredoxins in sample, stress degree information acquisition device, and stress degree determination method

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59201094A (en) * 1983-04-30 1984-11-14 カシオ計算機株式会社 Rhythm performer
JPS6076399U (en) * 1983-10-31 1985-05-28 ヤマハ株式会社 automatic performance device
JPS62187894A (en) * 1986-02-14 1987-08-17 ヤマハ株式会社 Electronic musical apparatus
JPH0755596Y2 (en) * 1986-09-01 1995-12-20 カシオ計算機株式会社 Electronic percussion input device
JPH02142895U (en) * 1990-04-27 1990-12-04
JPH02142894U (en) * 1990-04-27 1990-12-04
JPH02142893U (en) * 1990-04-27 1990-12-04
KR101226264B1 (en) * 2005-03-29 2013-01-25 유겐가이샤 얼티자임 인터내셔널 Biosensor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007240528A (en) * 2006-02-10 2007-09-20 Canon Inc Acquisition device for information as to concentration of thioredoxins in sample, stress degree information acquisition device, and stress degree determination method

Also Published As

Publication number Publication date
JPS5816693B2 (en) 1983-04-01

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