JPS54117089A - Preparation of lllysine oxidase - Google Patents
Preparation of lllysine oxidaseInfo
- Publication number
- JPS54117089A JPS54117089A JP2099378A JP2099378A JPS54117089A JP S54117089 A JPS54117089 A JP S54117089A JP 2099378 A JP2099378 A JP 2099378A JP 2099378 A JP2099378 A JP 2099378A JP S54117089 A JPS54117089 A JP S54117089A
- Authority
- JP
- Japan
- Prior art keywords
- lysine
- oxidase
- useful
- lllysine
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004316 Oxidoreductases Human genes 0.000 title 1
- 108090000854 Oxidoreductases Proteins 0.000 title 1
- 108010004733 L-lysine oxidase Proteins 0.000 abstract 3
- 241000233866 Fungi Species 0.000 abstract 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 abstract 2
- 241000223259 Trichoderma Species 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 241000223261 Trichoderma viride Species 0.000 abstract 1
- 230000000259 anti-tumor effect Effects 0.000 abstract 1
- 239000002246 antineoplastic agent Substances 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 208000034192 hyperlysinemia Diseases 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To prepare L-lysine α-oxidase having high substrate specificity to L- lysine, and having anti-tumor activity, by culturing fungi belonging to Trichoderma genus.
CONSTITUTION: Fungi belonging to Trichoderma genus and capable of producing L-lysine-oxidase, e.g. Trichoderma viride Y244-2-90 (FERM-P No.4256) ar aerobically cultured pref. in a solid medium usually at 20W30°C and pH4-8 for 3-25 days, and the objective L-lysine α-oxidase is separated from the water extract of the cultured product. The enzyme is useful as a reagent for the determination of L- lysine and a remedy for hyperlysinemia by its substrate specificity to L-lysine, as well as useful as an anti-tumor agent.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2099378A JPS54117089A (en) | 1978-02-27 | 1978-02-27 | Preparation of lllysine oxidase |
GB7905850A GB2015532B (en) | 1978-02-27 | 1979-02-19 | -lysine -oxidase |
DE19792906737 DE2906737C2 (en) | 1978-02-27 | 1979-02-21 | L-lysine α-oxidase, method for obtaining it and its use |
DE19792954471 DE2954471C2 (en) | 1978-02-27 | 1979-02-21 | |
CA322,273A CA1111790A (en) | 1978-02-27 | 1979-02-26 | L-LYSINE .alpha.-OXIDASE |
US06/015,179 US4234691A (en) | 1978-02-27 | 1979-02-26 | L-Lysine α-oxidase |
FR7904967A FR2418239A1 (en) | 1978-02-27 | 1979-02-27 | L-LYSINE A-OXYDASE, ITS PREPARATION AND USE FOR THE DETERMINATION OF L-LYSINE AND AS A THERAPEUTIC AGENT |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2099378A JPS54117089A (en) | 1978-02-27 | 1978-02-27 | Preparation of lllysine oxidase |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS54117089A true JPS54117089A (en) | 1979-09-11 |
JPS5627235B2 JPS5627235B2 (en) | 1981-06-23 |
Family
ID=12042639
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2099378A Granted JPS54117089A (en) | 1978-02-27 | 1978-02-27 | Preparation of lllysine oxidase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS54117089A (en) |
-
1978
- 1978-02-27 JP JP2099378A patent/JPS54117089A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS5627235B2 (en) | 1981-06-23 |
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