JPH09110710A - Dermal preparation for external use and bathing agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a new antiinflammatory agent and antiallergic agent having high safety or a dermal preparation for external use excellent in prevention of allergic dermatitic diseases or skin roughening and improvement thereof and a bathing agent. SOLUTION: This dermal preparation for external use utilizes excellent inhibiting actions on hyaluronidase and anticomplementary actions of an extract of a pericarp of Punica granatum L. Thereby, the extract can be utilized as an antiinflammatory agent and an antiallergic agent and is effective in prevention and treatment of various allergic diseases, further applicable to the dermal preparations for external use in all the forms (medicines, quasi-drugs or cosmetics) and bathing agents and manifests excellent effects on prevention and improvement in the skin and scalp having trouble such as allergic dermatitic diseases, atopic dermatitis, skin roughening or sensitive skin.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an anti-inflammatory agent and an anti-allergic agent containing a pomegranate extract, and an allergic skin inflammatory disease (eg, redness, eczema, edema, swelling) comprising the same. Etc.), rough skin, and sensitive skin, and prevent and improve troubles on the skin and scalp, and relates to a highly safe external preparation for skin and bath preparation.

The field of use thereof is various external and internal preparations in general, and specifically, liquid, emulsion, cream,
Ointments, gels, powders, granules, solids, foams, or ampoules, capsules, pills / pills, 1) external and internal medicines, 2) quasi drugs, 3) Topical or systemic skin cosmetics, 4) medicinal and / or cosmetic preparations applied to the scalp / hair (for example, shampoos, rinses, treatments, permanent liquids, hair styling products,
Hair dyes, hair nicks, hair growth and hair nourishing agents, etc.), 5) bath agents to be used by pouring into bath water, 6) oral compositions (toothpaste, gargle, gums and troches, etc.), 7) foods, etc. Can be mentioned.

[0003]

2. Description of the Related Art Today, hay fever, allergic rhinitis, bronchial asthma, atopic dermatitis, urticaria caused by drugs, etc. due to deterioration of environmental conditions, changes in lifestyle, increase in stress due to complication of social life, etc. An increasing number of people are suffering from allergic diseases and rough / sensitive skin. In particular, atopic dermatitis is attracting attention as one of the modern diseases, as its increase is exclaimed not only in children but also in a wide age group up to adults.

[0004] Allergy is usually to eliminate foreign substances (bacteria, pollen, mites, etc .: antigens) that have entered the body.
It is a disorder reaction due to immune function, which is a function that protects the body by producing biocomponents (antibodies, lymphocytes, etc.) that counter it, which sometimes becomes harmful to the body due to its hypersensitivity. .

Allergic reactions are classified into four types as shown in Table 1. Type I-III are called immediate type because allergic symptoms reach a maximum reaction quickly within minutes of antigen invasion, and type IV is called delayed type because it requires 24-48 hours.

[Table 1]

A brief explanation will be given below, focusing on the immediate allergy. IgE antibodies are mainly involved in type I allergic reaction. IgE against foreign antigens
The first step, in which antibodies are produced and IgE antibodies are fixed to mast cells and basophils and sensitization is established, the antigen is again contacted there,
The second step, in which the antigen-antibody reaction occurs in the sensitized cells and the chemical mediators such as histamine, serotonin, and SRS-A are released,
The released chemical messengers cause contraction of smooth muscle, enhancement of vascular permeability, edema, nerve stimulation, etc., and are generally classified into the third stage in which various allergic symptoms are expressed. Type I allergic diseases include atopic dermatitis, bronchial asthma,
Hives and pollen allergies are known.

The type II allergic reaction is a damaging reaction caused by destruction of cells of a tissue by IgG or IgM antibody. When an antibody binds to an antigen cell, the protein that composes the complement system is activated in a chain manner, and a complex that destroys the cell membrane is formed, so that the cell is destroyed. When the activated components of the complement system bind to cells, the antigen cells are captured, decomposed and removed by phagocytic cells such as polymorphonuclear leukocytes and macrophages. On the other hand, in the process of activation of the complement system, a substance called anaphylatoxin, which has an action of remarkably promoting the decomposition of antigen by phagocytic cells, is produced. Since it has the action of increasing the permeability of capillaries, the action of contracting smooth muscle, and the action of releasing histamine from mast cells, various allergic symptoms will be generated if it is excessively produced.

The type III allergic reaction is mainly caused by IgG antibodies, but its onset occurs by a complex mechanism involving many factors such as the complement system and polymorphonuclear leukocytes. An antigen-antibody conjugate produced by binding an antibody to an antigen is deposited on tissues such as blood vessels, kidneys, joints and skin to activate the complement system. When the amount of the conjugate is large, the complement system is excessively activated to produce anaphylatoxin, which increases vascular permeability and causes inflammation. In addition, when phagocytic cells work to remove antigen-antibody bound substances by the action of anaphylatoxin, degranulation reaction of lysosome occurs, and various types of hydrolytic enzymes such as protease in lysosome are released to the outside of the cell. Tissue and cell damage progresses and type III allergy develops.

[0009]

Most of the conventionally used antiallergic agents are agents for diseases caused by type I allergic reaction. For example, there are antispasmodics that relax smooth muscles, sympathomimetics that suppress hyperpermeability of capillaries, and antihistamines. These are drugs that act in the third stage, It was a symptomatic treatment, and most of them were synthetic medicines, and there was a problem in terms of side effects.

On the other hand, the most active research on antiallergic agents involved in type I allergic diseases shows that the agents that suppress the release of chemical mediators have an antagonistic effect on the released chemical mediators. The development of drugs that suppress the second stage, such as drugs, has not been found to have sufficient efficacy.

Further, no specific antiallergic agent for type II and type III allergic reactions has been found, and since complement is involved in these reactions, it has an action of suppressing the activity of complement. Research on drugs is underway.

By the way, the name of crude drug for pomegranate is called Ishigure,
In Chinese medicine, pericarp, bark, and root bark are called stone gall, and they are antidiarrheals.
It is taken as a tapeworm control drug, but the fact is that little research has been done on other pharmacological actions and uses.

[0013]

[Means for Solving the Problems] In view of such circumstances,
The present inventors have attempted to screen a substance that is a natural product for a substance that has excellent anti-inflammatory or anti-allergic action, has no side effects, and is mild to the skin.
As a result, they have found that pomegranate extract is highly safe and has a hyaluronidase activity inhibitory action and an anti-complement activity action, and completed the present invention. That is, an object of the present invention is to apply the pomegranate extract as an anti-inflammatory agent and an anti-allergic agent, and to add the pomegranate extract to allergic skin inflammatory diseases (eg, redness, edema, eczema, etc.) Another object of the present invention is to provide a skin external preparation and a bath preparation which are excellent in preventing or improving skin / scalp troubles such as rough skin and sensitive skin.

[0014]

BEST MODE FOR CARRYING OUT THE INVENTION The pomegranate used in the present invention is a pomegranate Punica granatum L. of the pomegranate family (Punicaceae).
Refers to. The extract is a pomegranate peel as it is or after being dried and extracted with a solvent. Examples of the extraction solvent include water, alcohols (eg, lower alcohols such as methanol and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, diethyl ether, dioxane, Esters such as acetonitrile and acetic acid ethyl ester, and organic solvents such as xylene, benzene, chloroform, and toluene can be used.

The extraction conditions are not particularly limited, but it is usually within the range of the boiling point of the solvent at normal temperature to normal pressure, and after extraction, it is filtered and concentrated and dried to be a solution or paste. It may be used in the form of gel, powder or the like. Further, in most cases, it can be used as it is, but if necessary, purification treatments such as deodorization and decolorization may be added within a range that does not affect its efficacy. As these means, an activated carbon column or the like may be used, and ordinary means generally applied depending on the type of extraction solvent may be arbitrarily selected.

The content of the pomegranate extract in the external preparation for skin of the present invention is not particularly limited, but in the case where it is intended to improve skin trouble in a short period of time, it is converted into solid content. It is preferable to mix it in any proportion within the range of 0.001 to 0.5% by weight. In the case of bath agents,
The formulation should be considered so that it will be poured into 200 to 300 L of bath water so that the concentration will be about the same.

On the other hand, for such various troubles, for example, skin cosmetics and hair preparations that are used on a daily basis, for the purpose of prevention only or gradually improving with cumulative effects. In addition, in the case of a preparation such as a bath agent, it is considered that the intended effect can be sufficiently expected even with the amount usually prescribed in the field (a solid content of about 0.0005 to 0.1% by weight).

In addition to the pomegranate extract, the anti-inflammatory agent and anti-allergic agent or the external agent for skin and bath agent of the present invention may optionally contain the following pharmaceuticals, quasi drugs, and cosmetics. It is possible to produce it in combination with a base or an additive which is usually used in the preparation such as.

[1] Various oils and fats Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil,
Cocoa butter, chamomile oil, carrot oil, cucumber oil, kukui nut oil, safflower oil, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil , Cocoa butter, palm oil, palm kernel oil, hazelnut oil, sorghum, coconut oil, beef tallow, tallow fatty acid, lard, hydrogenated oil, hydrogenated castor oil, etc.

[2] Waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax and the like.

[3] Mineral oil Liquid paraffin, petrolatum, paraffin, ozokeride, ceresin, microcristan wax, squalene, squalane, pristane, etc.

[4] Fatty acids Lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, natural fatty acids such as lanolin fatty acid, isononanoic acid, caproic acid , Synthetic fatty acids such as 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid, and isopentanoic acid.

[5] Alcohols ethanol, isopropyl alcohol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, 2-hexyldecanol,
Synthetic alcohols such as isostearyl alcohol and 2-octyldodecanol.

[6] Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol, etc.

[7] Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, etc.

[8] Metal Soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate, etc.

[9] Gum and water-soluble polymer compounds Arabic gum, benzoin gum, dammar gum, guaiac butter, Irish moss, karaya gum, tragacanth gum,
Kyarobugomu, Quincy de, agar, casein, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C ₂ ~C ₄₎ chitin or chitosan, low-molecular chitin or chitosan, chitosan salt, sulfated chitin Or chitosan, phosphorylated chitin or chitosan, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate and its salt, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, carboxyethyl cellulose sodium, hydroxyethyl cellulose, hydroxypropyl cellulose, nitro Cellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinylpyrrolidone, polyvinyl Le methacrylate, polyacrylate, polyalkylene oxide or crosslinked polymer thereof such as polyethylene oxide or polypropylene oxide, carboxyvinyl polymers, such as polyethyleneimine.

[10] Surfactant Anionic surfactant (carboxylate, sulfonate, sulfate ester salt, phosphate ester salt), cationic surfactant (amine salt, quaternary ammonium salt), amphoteric surfactant : Carboxylic acid type amphoteric surfactant (amino type, betaine type), Sulfate ester type amphoteric surfactant, Sulfonic acid type amphoteric surfactant, Phosphate ester type amphoteric surfactant, Nonionic surfactant (ether type non Ionic surfactants, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants, other surfactants (natural surfactants) Agent, protein hydrolyzate derivative,
Polymer surfactant, surfactant containing titanium and silicon,
Fluorocarbon surfactants).

[11] Various vitamins Vitamin A group: retinol, retinal (vitamin
A₁), Dehydroretinal (vitamin A_Two), Carochi
Lycopene (Provitamin A), Vitamin B group: Chia
Min hydrochloride, thiamine sulfate (vitamin B₁), Lvov
Rabin (vitamin B_Two ), Pyridoxine (vitamin
B₆), Cyanocobalamin (Vitamin B₁₂), Folic acid,
Nicotinic acid, Pantothenic acid, Biotin, Choline,
Inositols, vitamin C group: ascorbic acid and its
Derivatives of vitamin D group: Ergocalciferol (bi
Tamin D_Two), Cholecalciferol (vitamin D_Three),
Dihydrotaxosterol, Vitamin E group: Tocofellow
And its derivatives, ubiquinones, vitamin K group:
Tonadione (vitamin K₁), Menaquinone (vitamin
K_Two), Menadione (Vitamin K_Three), Menadiol (bi
Tamin K_Four)Such.

[12] Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, etc. and their sulfates, phosphates, nitrates, citrates, or amino acid derivatives such as pyrrolidone carboxylic acid.

[13] Various additives derived from plant- or animal-based raw materials. These are processes (for example, crushing, milling, washing, hydrolysis, etc.) which are conventionally carried out depending on the product type and form to be added. Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, powderization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. are arbitrarily selected and combined), and various materials are arbitrarily selected. You can select and serve. In the case of extraction, the solvent to be used may be selected in consideration of the usage (external use, bath use) of the product to be provided and the processing to be performed later, but usually, water or a water-soluble organic solvent (for example, , Ethanol, propylene glycol, 1,3-butylene glycol, etc.) is preferably used. However, when the inclusion of an organic solvent is not preferable depending on the application, water alone may be used, or ethanol that is easy to remove after extraction may be used and used alone or in an arbitrary mixture with water.

Additives derived from plant or animal raw materials are
When it is applied to external preparations such as skin care and hair care and bath agents, it protects skin and hair, moisturizes, improves touch and texture, imparts flexibility, relaxes stimulus, relieves stress due to aroma, and activates cells (cells). Aging prevention), suppression of inflammation, improvement of skin and hair quality, prevention and improvement of skin roughness, hair growth, hair growth, hair loss prevention, gloss impartment, washing effect, fatigue alleviation, blood flow enhancement, hot bath effect, etc. In addition to the artificial effect, it can be expected to have effects such as flavoring, thickening, antiseptic, and buffering. In addition to these, various cosmetic and pharmacological effects of each known raw material are expected, and by combining these, the intended effect is enhanced or a multifunctional effect is obtained. It is also possible to make it a product that you have.

In particular, by using the pomegranate extract of the present invention in combination with an extract of a plant already known to have anti-inflammatory / anti-inflammatory / anti-allergic action, an additive or synergistic anti-inflammatory / anti-inflammatory An anti-allergic effect can be expected. Examples of such a plant (herbal medicine) include Arnica, aloe (蘆 舆 舆), aloe vera, Prickly pear, turmeric (health), hemlock grass (summer hay), unshiu mandarin orange (orange), psyllium (orange), psyllium (car front) (Children, carcass grass), Hypericum (younger brother cut grass), Odrichosou (interrupted), Onami (blue ear), seaweed [green algae, brown algae, red algae], valerian roots, birch, chamomile, kawamuramogi (chinen cabbage), Licorice (licorice), chrysanthemum (urine root), chrysanthemum (chrysanthemum), yellowfin (yellow oak), cucumber, wolfberry (husks, husks, husks and leaves), kudzu (kudsk roots), gardenia (shanks), Kumazasa, Clara (bitter ginseng), Mulberry (mulberry leaves, mulberry bark), Keigai (Maggot, Mushroom), Genoshoko (Old grass), Kouhone (river bone),
Scutellaria baicalensis (yellow gon), Gosho strawberry (covered bonito), Copaiba, burdock (burdock, burdock), rice, rice bran, rice oil, comfrey (final herb), cherry leaves, sala chinensis (marsh), salvia, Sansho (sansho), Chia, Perilla frutescens, Chilemenjiso, Catamendiso (shiso leaf, shiso), linden, peony (peony medicine), janohiger (barley winter), jiuyu, jusdama, ginger (ginger), white birch, nematode , Izuki, horse chestnut, horse mackerel, elderberry, yarrow, mint, pearl oyster, senkyu, sendan, senburi (our medicine), rhubarb (daihuang),
Thyme, Dandelion, Touga (winter gourd), Toki (toki), Toki calendula, Dokudami (10 medicines), Tomato, Trolo mallow, Jujube (Otsuki), Elderberry (bone grafting), Carrot, Hibiscus, Hakamauraboshi (Bone crush) (Supplement), mint (thin leaf, thin leaf), pearl barley (Yokuinin), hamamelis, rose (rose), Hikiokoshi (prolonged life grass), Hishi (Hisashimi), juniper, loquat (Biwaki, loquat leaf), loofah,
Safflower (safflower), bodige, button (peony skin), hop, horse chestnut, swordfish, purple (purple root), melissa, peach, cornflower, eucalyptus, yukinoshita (tiger ear grass), mugwort (spike leaf), lavender, gentian, forsythia (forsythia) For example, rosemary, rosemary (mannenrou), Roman chamomile, waremoko (earthen worm), etc.

Other specific examples of plants (herbal medicines) that can be used in the field of cosmetics are eye (indigo leaf), blue vine (seifuto), Aotsutsurafuji (Kibo), acacia, red currant fruit, red grape, red megalois ( Akabashi),
Akane (Akane grass root), Akajiojiou (ground yellow), Agi (Awe), Akinire (resilient hide), Akebei (Kizu), Asa (Asako Jin), Morning glory (chocolate), Ashitaba (tomorrow), Azuki (Azuki) Red adzuki bean, Asenyaku (Asenyaku), Acerola, Anise fruit, Abemaki fruit, Avocado, Ama, Amacha (sweet tea), Achacha Crane, Amadokoro (Tamatake), Ayagasayuri (shellfish), Almond, Altea, Apricot (Apricot kernel), Ansokkou. (Benzoic incense), igakouzorina (ground bile), knotweed (tiger cane root), strawberry, fig (flowerless) or its leaf, hibiscus (winter aoi), ginkgo (gingko leaf, ginkgo), himehimehagi (distant), carob, Rice seeds or seed coats, Innodo seeds, nettles, mussels, ylang-ylang, ginkgo (gingko leaves, ginkgo), fennel (boiled incense), ukiyaga (Sanryo), Uguisu Kagura fruit, Usvena mallow, Usbenitachiaoi, Usubasaishin (spicy), Udo or Shishiudo (coast, independent activity, Karaimo), Ume (karaume) or its flesh, Vladimir oak, Urakushi, Estragon, Ezo Kogi ( Ezo Goka), Enoki, Ebisugusa (Keimeiko), Elderberry fruit, Elemi, Enju (Sophora, Sophora rice), Ougi (Yellow 耆), Okarasuri (Karonin), Otsutsurafuji (self-defense),
Hazel (Haruko), Obana okera (Shiroyaku), Obayashishabushi fruit, Oleum hawthorn (Yamashiko), Barley (barley), Oguruma (overturning), Okra fruit, Okera (white syrup), Panax ginseng (Red ginseng, carrot), Onamomi Blue ear), Onigurumi, Oninogaraga (Tianma), Oniyuri (Yuri), Ononis, Halibut (Ryaku-no-tsumaki), Ominaeshi (defeated soy sauce), Dutch strawberry, Dutch mustard, Dutch jelly, Dutch honeywort, olive, oregano, orange fruit Or pericarp, kaisekiou (ripe ground yellow), cacao seeds, oyster or its leaves (persimmon), Kagizura (fishing hook), cassia, casino berries (cowberry), guajutu (calyx), kashiwa (camellia tree, mallet leaf). ), Cascarilla, cucumber (Kinshato), pumpkin, kapok tree seed, kahokusansho (pepper), gama (ginger) Tetrapanax (Akebia),
Crow cucumber (crow melon), oats, oats, crow bischak (half-summer), guarana seeds, karaya, karin (wood melon), kawayanagi, kawaratake, kansui (sweetening), candelilla, kanto, canna, raspberries, kiwi fruit, kyokyo (kikyo, bellflower) Root), Catalpa (Azusa fruit), Rumex communis (Yotei root), Kizuta, Yellow aloe, Yellow mint, Quercus chinensis, Kibanana cypress (Camellia), cabbage, cabbage, unripe fruit, Kiraja saponaria, Kiraya, Quercus, Quincus, Kinmizu Hiki (Sen Tsurugusa),
Guayule, Quercus infectria (gallic), kukuinut, kusakujizura (tenmon winter), camphor tree, gooseberry fruit, kukunugi (chinensis), kumazasa, kutsutsuura (horse whiskers), cranberry fruit, chestnut or its astringent skin, curculigo latifolia fruit ， Grapefruit fruit ， Black buckthorn ， Black gadget （Necessity of rescue） ， Black-buckwheat sorghum （Life-prolonging grass） ， Chlorella ， Gwaba fruit ， Caito (copricot flower, corolla) ， Keirinsaishin （spicy）, Laurel ， Gentiana, cilantro fruit, Koushinbara （ Monthly flower), Kosui mint, Kozo fruit,
Black tea, Kouhon (straw, Karamoto), Kouryan, Cowberry (Koshitachi), Kogomebaotogisou, coconut fruit, goshuyu (goshuyu), pepper (pepper), coffee beans, kobushi (spicy), kobuttonur, sesame (sesame). Sesame flower (genus), wheat (wheat), cola acminata seeds, cola vera seeds, fenugreek fruit, colombo, conzulango, kelp, konjac, saxima buttonzul (weapon), cherry blossoms, fruits, bark (cherry bark) ), Sasa, Sasayuri (lily), Sasanqua, Sagemodaka (Sawarai), Sweet potato, Sugarcane, Sugar beet, Sanbutsunajume (Satsumejiren), Saffron (Bandflower, West safflower), Pomelo fruit, Saboursou, Salmonberry fruit, Salvia, Sawakiki (Yamakage greens), Soiled walnut leaves, Sankirai (Yama Return), Hawthorn (Mountain) Child), Panax ginseng (Panax notoginseng), Cornus (mountain Fructus), et rhizoma sophorae tonkinensis (Yamamamene),
Cyanoki fruit, Shiitake mushrooms, Ziou (ground yellow), Zion (Shien), Digitalis, Shikunshi (Koshikushi), Shishiudo (single live, Tang solo), Rosewood, Chinese reinforce (Renka), Pleurotus cornucopia (Chrysanthemum), Shimosa, Potato, Shazin (Shadow). Sasan), white oak seeds, white-billed butterflies, white-white iris, white-spotted ivy flowers, white-white minamiten (southern nuts), palm fruits, shobu (irises, iris roots), shozuku fruits, cinchona saxsilbra, cinnamon, watermelons (watermelons) , Honeysuckle (gold and silver flower, Shinobi winter), sorrel (acidic acid), swimberry fruit, strawberry fruit, horsetail (question), stevia, plum fruit, pine nuts conical, larch pine, walnut, hawthorn, pear fruit ， Salicornia vulgaris, Saccharomyces, Saccharomyces , Rose, Astragalus vulgaris, Mistletoe, Astragalus oleracea, Horseradish, Horseradish, Stone stalk (Stone root), Mallow, Senega, Seri, Celery, Senna fruit or leaf, Senninso (Large flower), Sawka (grass fruit), Buckwheat seed fruit , Somei potato, radish, soybean, Daidai (orange skin, Minami), Takatodai (Ogi), Takawarabi (Gourd), dark sweet cherry fruit, Tachibako (Hyurika), Tachibana (Tachibana), Tachinagi, Tamarind seeds, onion , Tamshiba (spice), cod or root bark, tanjin (danshen), dammar, cherry fruits, chigaya or its root (kaya root), chicory, clove (clove, clove), ginseng (chondria), pearl millet (daihuang) , Ginseng (garden), Ginseng (carrot) , Ginseng (agar leaf), Chomaimaitake (bottle), Camellia japonica, camellia, Centella asiatica, Clover (lacquer grass), Tsuyakusa (duckweed grass), Azuki bean (red adzuki bean), Tsurukudami (Japanese plum crow), Tsuruna (蕃) , Ginseng (Folia ginseng), Tsuwabuki, Dako, Ginseng, Duberry fruit, Agar tree, Tendaiyaku (crow), Capsicum (Pepper), Maize or corn hair (Nanban-hair), Squirrel mochi (Sadako), Ginseng (dragon gall) ), Tokon (root), Carrot (Ginseng),
Eucommia vulgaris (Tochu, Tochu leaf), Tomato, Tragant, Triacanthus seeds, Tormentilla, Doronoki, Nigeria berry fruit, Chinese yam (mountain drug), Nagikadada, Naginatakouju, Nazuna, Rape, Natsumikan, Naniwaibara (Narukoyuri), Narukoyuri (Yellow sprout) ), Peanuts (peanuts), nanten (southern nuts), nigaki (bitterwood), sagebrush (bitterwood), nutmeg, nikkei (cinnamon), leeks (sparrow), garlic (garden), nurude (quintet), leek, Thistle (Oyagi), Noibaru (Yokohama), Yarrow, Nodake (Phuket), Novara, Nomomo, Palm, Pineapple fruit, Hakatayuri (lily), Hakusen (white moss peel), Hakuruberry fruit, Hakobe (combination), Hazel (hazel) Haruko), Hasiridokoro (roth root), Basil, lotus (lotus meat, lotus), Hasunage (mother) Parsley, barley, Batata, irresistible force (Take茹), Pachori, Anemarrhenae asphodeloides (knowledge mother),
Bananas, Hana mint, Vanilla bins, Papayas, Hahacogusa (mouse koji), Habu grass, paprika, Hamago (vine vine), Hamasuge (scented corn), Hamabishi (Situriko), Hamanasu (mai a flower), Hamabofu (Hamabofu) ， Parietalia ， Harunire (inner skin, Elm white skin, Elm leaf), Panoki,
Hyogi (dried dry), carrot ginseng (participant), pecan nuts, hemlock (stone garlic, vine pearl scent), Hikiokoshi (prolonged life grass), pistachio, beet, tsutowa (stone reed), Hinata no Kozuchi (cow knee), cypress, Hiba, Castor, Sunflower, Bell Pepper, Himegama (scented), Himematsutake, Pimenta fruit, Hiyu fruit, Birodoaoi, Hiroha Okinagusa (Bald head), Hirohase Nega (Senega), Areca (Large belly skin), Minami Fujito, Futoka ，
Butterbur (subsistence winter flower), coltsfoot dandelion (subsistence winter flower, subsistence winter leaf), fujibakama (orchid grass), wisteria beans (flat bean), butcher bloom, grapes, beech, Fuyusuminatsukusatake (caterpillar), Brazilian licorice, black currant fruit, blackberry Fruit, plum fruit, fluceraria, blueberry fruit, prunes, blond psyllium, bundou (mung bean), belladonna, berry fruit, boysenberry fruit, bowfu (windproof), spinach, physalis (Torone), honoki (bamboo, Japanese Atsushi) ), Bokeh (wood melon), Hosobaokera (sour), Hosobabarengiku, jojoba, white berry fruit, hon apricot (apricot kernel), honshimeji, maizurutennansho (tenan star), maou (maou), macadamia nuts, macri (seagrass), Madake (boiled bamboo),
Matatabi (wooden Tate), Matsukasa, Matsuhodo (boiled peony), Marigold, Marvanojazin (Apricot ginseng), Mulberry fruit, quince, mango, mangosteen, manshu gurumi, mandarin fruit, mantake mushroom (reishi), citrus fruit (concentrated fruit) ), Mishima Psycho (Saiko), Mizokakushi (Half-sided lotus), Misohagi (Sengansai), Mitsugashiwa, Mitsuba, Mitsubahamago (vine vine), Midorihakka, Mimosa, Myouga, Miracle Fruit, Myrrh, Myrobalan, Mukuge (Musage) (Kaki), Mukunoki, Purple corn, Swordfish (Medicinal herb), Mebuki, Melaleuca, Merilotus, Melon fruit, Mokoyomogi, Mosouchi, Magnolia (Spicion), Mockweed (wood aroma), Momijibadaiou, Peach fruit, seed (Momojin), Bean sprouts, morelo cherry cherry, moroheiya, Kawamurasaki yam, yakchi (maschi), cornflower, cornflower, yashabushi (arrow wheel), yachiyanagi, yadede (octagonal metal plate), mistletoe (willow parasite), willow leaf, yam pepper, yabukoji (purple gold cow), pokeweed (trade) Land), Yamano yam (mountain medicine), Yamanoki (Yamaharu), bayberry (Yangmei peel), Yucca brevifolia, yuzu fruit, lily, yorogusa, lime fruit, rye, lakanka fruit, raspberry fruit, peanut, longan (dragon eye meat) , Green tea, apple fruit, rubus suabisimus, lettuce, red currant fruit, lemon fruit, lemongrass, astragalus, wax palm, loganberry fruit, rosehip, wakame, wasabi, etc.

Materials derived from animal-derived raw materials include chicken cob extract, bovine / human placenta extract, swine / cow stomach or duodenum or intestinal extract or its degradation product, water-soluble collagen, acylated collagen and the like. Collagen derivative, collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, silk protein, silk protein hydrolyzate, pig / cattle blood cell protein hydrolyzate (globin peptide),
Pig and bovine hemoglobin degradation products (hemin, hematin, heme iron, etc.) and milk can be used.

[14] Microbial culture metabolites Yeast metabolites, yeast extract, rice fermentation extract, rice bran fermentation extract, Euglena extract, lactic acid fermented product of skim milk powder, etc.

[15] α-hydroxy acid Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid, etc.

[16] Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

[17] UV absorber p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative,
Camphor derivative, furan derivative, pyrone derivative, nucleic acid derivative, allantoin derivative, nicotinic acid derivative, vitamin B ₆ derivative, oxybenzone, benzophenone, guaiazulene, shikonin, baicalin, baicalein,
Berberine etc.

[18] Antioxidant Ascorbic acid and its salts, stearic acid ester, tocopherol and its ester derivatives, nordihydroguaselethenoic acid, butylhydroxytoluene (BHT),
Butylhydroxyanisole (BHA), parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol and the like.

[19] Anti-inflammatory agent Ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-
Camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts and the like. still,
By using the pomegranate extract of the present invention in combination with the above-mentioned anti-inflammatory agent, an additive or synergistic anti-inflammatory / anti-allergic action can be expected.

[20] Astringents Lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannin Acid etc.

[21] Thickener Hydroxyethyl cellulose, methyl cellulose, ethyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, gum arabic, polyvinyl alcohol, polyvinylpyrrolidone, polyvinyl methacrylate, polyacrylate, carboxyvinyl polymer,
Carrageenan, pectin, alginic acid and its salts, casein, gelatin, etc.

[22] Moisturizers Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid and its salts, polyethylene glycol, chondroitin sulfate and its salts, water-soluble chitin or chitosan derivatives, sodium lactate, etc.

[23] Bactericidal / Disinfectant Acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or its hydrolyzate.

[24] Fragrances Natural animal fragrances such as musk, civet, catrium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, karaway essential oil, cardamom essential oil, Guayak Wood Essential Oil, Cumin Essential Oil, Black Letter Essential Oil, Cayenne Essential Oil, Cinnamon Essential Oil, Geranium Essential Oil, Copaiba Balsam Essential Oil, Coriandel Essential Oil, Perilla Essential Oil, Cedarwood Essential Oil, Citronella Essential Oil, Jasmine Essential Oil, Gingergrass Essential Oil, Cedar Essential Oil, Spearmint Essential Oil, Western Mint essential oil, large scented essential oil, tuberose essential oil, Choji essential oil, orange essential oil, winter green essential oil, true balsam essential oil, butchery essential oil, rose essential oil, palmarosa essential oil, cypress essential oil, hiba essential oil,
Sandalwood essential oil, Petitgren essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

[25] Dye / Colorant Red cabbage dye, red rice dye, madder dye, annatto dye, squid dye, turmeric dye, ende dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, whole buckwheat pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

[26] Sweeteners Sugar, sweet tea, fructose, arabinose, galactose, xylose, stevia, mannose, maltose, honey, glucose, miraculin, monerin, etc.

[27] Nutrient enhancer Calcium shell calcium, cyanocorabamine, yeast, wheat germ, egg yolk powder, hemicellulose, etc.

[28] Other hormones, sequestering agents, pH adjusting agents, chelating agents, antiseptic / antifungal agents, cooling agents, stabilizers, emulsifiers, kinetic agents
Vegetable protein and its degradation products, animal / vegetable polysaccharides and its degradation products, animal / vegetable glycoprotein and its degradation products, blood flow promoters, anti-inflammatory / anti-allergic agents, cell activating agents, keratolytic agents, A wound healing agent, a foam-enhancing agent, an oral agent, a deodorant / deodorant, an enzyme, etc. may be used in combination as required to give various preparations as described above.

The anti-inflammatory and anti-allergic agents of the present invention, or the external preparations for skin and the bath preparations may have any dosage forms, such as liquids, emulsions, creams, ointments, gels, powders, granules and solids. It can be used by blending it with a foamy or ampoule-shaped, capsule-shaped, pill / tablet-shaped external or internal drug, quasi-drugs, skin / hair cosmetics, and bath preparation.

Specifically, external / internal medicinal preparations, basic cosmetics such as lotions, emulsions, creams, ointments, oils and packs, facial cleansers and skin cleansers, shampoos, rinses, hair treatments, hair styling products, Perms, hair dyes, hair tonics, hair cosmetics such as hair growth and hair nourishment, foundations, lipsticks, blushers, eye shadows, eye liners,
Make-up cosmetics such as mascara, liquid / powder / solid bath agents, toothpaste, mouthwash, mouthwash, hygiene products,
Examples include wet tissues and the like, and can be applied to other general food and drink.

Regarding the method of adding the pomegranate extract of the present invention to the external preparation for the skin and the bath preparation, it may be added in advance or during the preparation, and it may be appropriately selected in consideration of workability. Good.

[0054]

EXAMPLES Next, the present invention will be described in more detail with reference to production examples, test examples, and formulation examples, but it goes without saying that the present invention is not limited to these.

(Production Example 1) 100 g of pomegranate skin was immersed in 1 L of water, extracted at room temperature for 3 days and night, and suction filtered to obtain an extract (dry solid content 3.0 to 6.0% by weight).

(Production Example 2) 100 g of pomegranate skin was gently extracted with 1 L of an equal volume mixture of 30% ethanol solution and 20% 1,3-butylene glycol solution at 50 ° C. for about 6 hours,
An extract (dry solid content 3.0-6.0% by weight) is obtained.

(Production Example 3) 100 g of pomegranate skin was mixed with hexane.
After soaking in 500 ml for one day and night, the solvent was distilled off, and the obtained paste-like substance was redissolved in 1 L of 30% 1,3-butylene glycol solution and suction filtered to obtain an extract (dry solid content 3.0 to 6.0% by weight). ) Get.

(Test 1) Measurement of inhibitory activity on hyaluronidase activity Hyaluronidase is a hydrolase of hyaluronic acid distributed in connective tissue, and is activated during inflammation and destroys the matrix of connective tissue to inhibit cells and blood vessels of inflammatory system. Is believed to play a role in increasing the permeability of It is also known as a inflammatory enzyme, and is also used as a inflammatory agent that experimentally induces acute edema. Furthermore, it is reported that it is inhibited by anti-inflammatory agents and anti-allergic agents (inflammation, Vol. 4, No. 4, P. 437 (1984)). Therefore,
By measuring the activity of this enzyme, it is possible to evaluate anti-inflammatory and anti-allergic effects. In this test, the inhibitory effect of the extract obtained in Production Example 1 was examined with reference to the Morgan-Elson method.

(Test Method) a. Sample Extraction solution was evaporated under reduced pressure to remove the solvent, and then purified water
It was redissolved so as to be 0.5% by weight and used for the test. still,
The same concentration of dipotassium glycyrrhizinate was used as a positive control. b. Measurement of hyaluronidase activity Hyaluronidase solution (final concentration 0.4 mg /
0.05 ml), leave it at 37 ℃ for 20 minutes, and then add Compound48 /
80 solution (final concentration 0.1 mg / ml) was added, and the mixture was allowed to stand at 37 ° C for 20 minutes, and then hyaluronic acid solution (final concentration 0.4 mg / m
l) 0.25 ml was added and left at 37 ° C. for 40 minutes. After stopping the reaction by adding 0.1 ml of 0.4N sodium hydroxide solution, 0.8M
0.1 ml of potassium borate solution was added and heated in boiling water for 3 minutes. After cooling to room temperature, 3 ml of a 1% p-dimethylaminobenzaldehyde acetic acid solution was added, the mixture was allowed to stand at 37 ° C. for 20 minutes, and then the absorbance at 585 nm was measured. In addition, the one in which purified water was added instead of the sample was used as a control, a blank containing no enzyme was set for each sample and the control, and the hyaluronidase inhibitory activity rate was calculated by the following formula.

(Equation 1)

(Test Results) As shown in Table 2, the pomegranate extract of the present invention was confirmed to have an excellent hyaluronidase activity inhibitory activity comparable to that of dipotassium glycyrrhizinate, which is widely known to have anti-inflammatory and anti-allergic activity. Therefore, it can be judged that it has anti-inflammatory and anti-allergic effects.

[Table 2]

(Test 2) Measurement of anti-complement activity The complement system is greatly involved in type II and type III allergic reactions. In addition, when complement is activated, it causes degranulation from mast cells and causes type I allergy-like symptoms.
Therefore, the effect on allergy can be evaluated by measuring the complement activity. In this test, Production Example 1
The extract obtained in 1. was examined using an anti-complement activity measuring method using the hemolytic reaction of sensitized red blood cells as an index.

(Test method) a. Gelatin / Veronal buffer (GVB ²⁺ ) Sodium chloride 1.7g, barbital 0.115g, barbital sodium 0.075g, calcium chloride 0.015g, magnesium chloride 0.010g, gelatin 0.2g, purified water 100ml are mixed to adjust pH 7.5. Then, the total volume was adjusted to 200 ml with purified water. b. Sheep erythrocyte (SRBC) suspension The sheep blood cells were centrifuged at 2,000 rpm for 5 minutes, washed three times with physiological saline, and GVB ²⁺ was added to the residue to prepare a 10% SRBC suspension, and finally SRBC. 3.05 in 0.25 ml suspension
When 0.1 ml of 0.1% sodium carbonate solution was added for complete hemolysis, the absorbance at 540 nm was adjusted to 0.455. c. 0.2 ml of anti-SRBC mouse serum 10% SRBC suspension was intravenously injected into the tail of an IVCS male mouse, and 4 days after that, blood was collected and serum was separated.
It was used after being diluted 40-fold with B ²⁺ . d. Complement Guinea pig fresh serum was diluted 20-fold with GVB ²⁺ and used. e. After the solvent was distilled off from the sample extract under reduced pressure, the sample extract was redissolved in purified water to a solid content concentration of 0.5% by weight and used for the test.
Dipotassium glycyrrhizinate was used as a positive control. f. Measurement of anti-complement activity 0.2 ml sample to 0.5 ml GVB ²⁺ and 0.5 ml anti-SRBC serum, S
After 0.25 ml of RBC suspension and 0.25 ml of complement solution were added successively, the reaction was carried out for 60 minutes in a constant temperature bath at 37 ° C. In ice water 10
After allowing the reaction to stand for a minute to stop the reaction, the reaction solution was centrifuged at 2,000 rpm for 10 minutes to separate unlysed red blood cells, and the OD value at 540 nm of the supernatant was measured. In addition, a sample containing purified water instead of the sample was used as a control, and a blank containing no serum was set for each sample and the control, and the complement inhibition rate (= anti-complement activity) was determined by the following formula.

(Equation 2)

(Test Results) As shown in Table 2, it was confirmed that the pomegranate extract of the present invention has a strong anti-complement activity far exceeding that of dipotassium glycyrrhizinate.
Expected to have the effect of suppressing the development of type-allergic symptoms.

(Test 3) Safety test (1) Primary skin irritation test The extracts obtained in Production Examples 1 to 3 had a dry solid content of about 20.
It was prepared so as to have a weight percentage, and this was applied to the skin of rabbits (3 animals per group, body weight of about 3,800 g) with the hair removed from the back. The evaluation was carried out 24, 48, and 72 hours after application by using the erythema and edema as indices by the primary irritation scoring method. As a result, all animals were judged to be negative without any erythema or edema. (2) Skin cumulative irritation test The extract obtained in Production Examples 1 to 3 had a dry solid content of about 20.
Prepared so that the weight percentage becomes%, and remove the hair from the flank (2 x 4
cm ² ) Hartley guinea pig (female, 5 per group,
Skin (body weight: around 320 g) was applied once a day, 5 times a week, at 0.5 ml / animal. The application was carried out for 4 weeks, and the hair removal was performed on the last application day of each week. The determination was performed on the day following the last day of each week, using the erythema and edema as indices by the primary stimulus scoring method. As a result, in all animals, 1-
Over 4 weeks, no erythema or edema was observed and the result was negative.

(Formulation Example 1) Face-wash cream 1. Stearic acid 10.0% by weight 2. Palmitic acid 10.0 3. Myristic acid 12.0 4. Lauric acid 4.0 5. Oleyl alcohol 1.5 6. Lanolin derivative (EO adduct) 1.0 7. Glycerin 18.0 8. Potassium hydroxide 6.0 9. Pomegranate extract according to Production Example 1.0 10. Preservative appropriate amount 11. Fragrance appropriate amount 12. Purified water 100 remainder

(Formulation Example 2) Soft lotion 1. Glycerin 5.0 wt% 2.1,3-butylene glycol 6.0 3. Polyoxyethylene sorbitan monolaurate (20E.O.) 1.0 4. Methyl paraoxybenzoate 0.2 5. Ethanol 10.0 6. Pomegranate extract according to Production Example 1.5 7. Preservative appropriate amount 8. Perfume appropriate amount 9. Purified water 100 remainder

(Formulation Example 3) Emulsion 1. Stearic acid 2.0 wt% 2. Cetanol 1.5 3. Vaseline 3.0 4. Lanolin alcohol 2.0 5. Liquid paraffin 10.0 6. Polyoxyethylene monooleate (10E.O.) 2.0 7. Glycerin 3.0 8. Propylene glycol 5.0 9. Triethanolamine 1.0 10. Pomegranate extract according to Production Example 1.5 11. Preservative proper amount 12. Perfume proper amount 13. Purified water 100 residue

(Formulation Example 4) Emollient cream 1. Stearic acid 2.0% by weight 2. Stearyl alcohol 7.0 3. Reduced lanolin 2.0 4. Squalane 5.0 5. Octyldodecanol 6.0 6. Polyoxyethylene cetyl ether (25E.O.) 3.0 7. Lipophilic glyceryl monostearate 2.0 8. Propylene glycol 5.0 9. Pomegranate extract according to Production Example 2.0 10. Preservative appropriate amount 11. Perfume appropriate amount 12. Purified water 100 remaining amount

(Formulation Example 5) Pack 1. Polyvinyl alcohol 15.0% by weight 2. Carboxymethylcellulose sodium 5.0 3. Propylene glycol 3.0 4. Ethanol 10.0 5. 50% pomegranate extract with ethanol solution 1.0 6. Preservative proper amount 7. Perfume proper amount 8 .Remaining amount of purified water 100

(Formulation Example 6) Cream foundation (O / W type) 1. Stearic acid 4.0% by weight 2. Glycerin monostearate 3.0 3. Cetanol 1.5 4. Isopropyl myristate 7.0 5. Liquid paraffin 10.0 6. Salix beeswax 3.0 7. Triethanolamine 1.5 8. Glycerin 3.0 9. Bentonite 1.0 10. Titanium oxide 8.0 11. Kaolin 3.0 12. Talc 3.0 13. Color pigment 1.0 14. Dried powder of pomegranate extract according to Production Example 0.1 15. Preservative proper amount 16. Perfume proper amount 17. Purified water 100 remaining amount

(Formulation Example 7) Shampoo 1. Lauryl sulfate triethanolamine (40%) 15.0% by weight 2. Polyoxyethylene lauryl ether (2E.O.) sodium sulfate (70%) 5.0 3. Lauric acid ethanolamide 2.0 4. Ethylene glycol monostearate 2.0 5. Disodium edetate 0.1 6. Pomegranate extract according to Production Example 2.0 7. Preservative appropriate amount 8. Perfume appropriate amount 9. Purified water 100 remaining amount

(Formulation Example 8) Conditioning rinse 1. Stearyl trimethyl ammonium chloride 1.5% by weight 2. Cetanol 2.0 3. Camellia oil 1.0 4. Lanolin fatty acid 1.0 5. Propylene glycol 6.0 6. Polyoxyethylene stearyl ether 1.0 7. Production example 2 Pomegranate extract 1.5 8. Perfume suitable amount 9. Purified water 100 remaining amount

(Formulation Example 9) Hair tonic 1. L-menthol 0.2 wt% 2. Ethanol 32.5 3. Polyoxyethylene hydrogenated castor oil 0.5 4. Propylene glycol 5.0 5. Diphenhydramine hydrochloride 0.3 6. Pomegranate extract according to Production Example 1 2.0 7. Preservative proper amount 8. Perfume proper amount 9. Purified water 100 remaining amount

(Formulation Example 10) Granular bath agent 1. Sodium hydrogen carbonate 50.0% by weight 2. Anhydrous sodium sulfate 30.0 3. Sodium chloride 15.0 4. Dried powder of pomegranate extract according to Preparation Example 5.0 5. Appropriate amount of pigment 6. Fragrance suitable amount

(Test 4) Use effect test The effect of actually using the external preparation for skin or bath agent of the present invention was examined. Use test is worried about skin diseases such as eczema, urticaria and atopic dermatitis 2-30
Ten panelists were 10 years old, and twice a day, morning and night, after washing the face, an appropriate amount of the cream of Formulation Example 4 was applied to the face for 1 month. Also, for 10 persons (2 to 10 years old) who have similar skin inflammation on the scalp and hairline, a use test was conducted by applying the hair tonic of Formulation Example 9 to the scalp for 1 month after washing the hair daily. went. Furthermore, for the bath agent of Prescription Example 10, a bath agent for one month was used as a control against 20 people aged 0 to 45 having dry skin with dryness, skin diseases such as eczema and urticaria, and atopic dermatitis. I had them take a bath in the melted bath water and tried a monitor test. As a control, for creams and hair nicks, purified water was used after removing the pomegranate extract, and as the bath agent, the one prepared by the same method was used, which was corrected with anhydrous sodium sulfate instead of the pomegranate extract.

The condition of the skin or scalp one month after the start of use was evaluated according to the following criteria.・ Effect of improving skin (scalp) inflammation Effective: Redness and itch associated with inflammation such as eczema and rough skin improved somewhat Effective: Redness and itch associated with inflammation such as eczema, rough skin slightly improved Ineffective: Before use And no change

The test results are shown in Table 3 (the numbers are the number of people).
No one complained of abnormalities on the skin or scalp during the period of use.

[Table 3]

As is clear from the results shown in Table 3, the use of the external preparation for skin and the bath preparation containing the pomegranate extract of the present invention was confirmed to have an excellent effect of improving inflammation of the skin and scalp.

[0079]

INDUSTRIAL APPLICABILITY The pomegranate extract of the present invention has a hyaluronidase activity inhibitory action and an anti-complement activity action, and is highly safe to the skin. Therefore, it can be used as an anti-inflammatory / anti-allergic agent and is effective in the prevention and treatment of various allergic diseases such as eczema, urticaria, atopic dermatitis, allergic rhinitis and hay fever. Further, it can be applied to preparations in any form (medicines, quasi drugs, cosmetics), and the skin external preparation and bath preparation according to the present invention are
Use for the purpose of prevention and improvement of allergic skin inflammatory diseases (eg, redness, eczema, edema, swelling, etc.) and atopic dermatitis, rough skin, and sensitive skin with problems such as sensitive skin. And others,
It can also be applied to oral compositions and foods.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical display location A61K 7/06 A61K 7/06 7/075 7/075 7/08 7/08 7/48 7 / 48 7/50 7/50

Claims (2)

[Claims] 1. An anti-inflammatory agent and an anti-allergic agent comprising a pomegranate extract. 2. A skin external preparation and a bath preparation containing a pomegranate extract.