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JPH08504599A - ニトリルヒドラターゼ活性を有する酵素、それらの製造のための遺伝学的ツール及び宿主微生物、並びに該酵素を用いる加水分解工程 - Google Patents

ニトリルヒドラターゼ活性を有する酵素、それらの製造のための遺伝学的ツール及び宿主微生物、並びに該酵素を用いる加水分解工程

Info

Publication number
JPH08504599A
JPH08504599A JP7506261A JP50626195A JPH08504599A JP H08504599 A JPH08504599 A JP H08504599A JP 7506261 A JP7506261 A JP 7506261A JP 50626195 A JP50626195 A JP 50626195A JP H08504599 A JPH08504599 A JP H08504599A
Authority
JP
Japan
Prior art keywords
enzyme
nitrile
nitrile hydratase
sequence
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP7506261A
Other languages
English (en)
Japanese (ja)
Inventor
セルブロー，エディット
レビシル，ソフィ
ペートル，ドミニク
スーブリエ，ファビエンヌ
Original Assignee
ローヌ−プーラン シミ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ローヌ−プーラン シミ filed Critical ローヌ−プーラン シミ
Publication of JPH08504599A publication Critical patent/JPH08504599A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP7506261A 1993-08-10 1994-08-09 ニトリルヒドラターゼ活性を有する酵素、それらの製造のための遺伝学的ツール及び宿主微生物、並びに該酵素を用いる加水分解工程 Pending JPH08504599A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR93/09990 1993-08-10
FR9309990A FR2708936B1 (fr) 1993-08-10 1993-08-10 Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes.
PCT/FR1994/000993 WO1995004828A1 (fr) 1993-08-10 1994-08-09 Enzymes a activite nitrile-hydratase, outils genetiques et micro-organismes hotes permettant leur obtention et procede d'hydrolyse mettant en ×uvre lesdites enzymes

Publications (1)

Publication Number Publication Date
JPH08504599A true JPH08504599A (ja) 1996-05-21

Family

ID=9450218

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7506261A Pending JPH08504599A (ja) 1993-08-10 1994-08-09 ニトリルヒドラターゼ活性を有する酵素、それらの製造のための遺伝学的ツール及び宿主微生物、並びに該酵素を用いる加水分解工程

Country Status (5)

Country Link
EP (1) EP0665889A1 (fr)
JP (1) JPH08504599A (fr)
CA (1) CA2146656A1 (fr)
FR (1) FR2708936B1 (fr)
WO (1) WO1995004828A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006062189A1 (fr) * 2004-12-09 2006-06-15 Asahi Kasei Kabushiki Kaisha Transformant exprimant la nitrile hydratase

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997012964A2 (fr) * 1995-10-06 1997-04-10 E.I. Du Pont De Nemours And Company Fragments d'acide nucleique codant une nitrile hydratase stereospecifique et des enzymes amidases ainsi que des microorganismes de recombinaison exprimant ces enzymes utiles pour la production d'amides et d'acides chiraux
GB9525372D0 (en) * 1995-12-12 1996-02-14 Allied Colloids Ltd Enzymes, their preparation and their use in the production of ammonium acrylate
US5728556A (en) * 1996-03-14 1998-03-17 E. I. Du Pont De Nemours And Company Production of ω-cyanocarboxamides from aliphatic α,ω-dinitriles using pseudomonas putida-derived biocatalysts
US5866379A (en) * 1997-01-28 1999-02-02 Novus International Enzymatic conversion of α-hydroxynitriles to the corresponding .alpha.
US20040225116A1 (en) 2003-05-08 2004-11-11 Payne Mark S. Nucleic acid fragments encoding nitrile hydratase and amidase enzymes from comamonas testosteroni 5-MGAM-4D and recombinant organisms expressing those enzymes useful for the production of amides and acids
WO2006049618A1 (fr) * 2004-11-01 2006-05-11 E. I. Du Pont De Nemours And Company Nitrile hydratase et amidase de comamonas testoteroni 5-mgam-4d
BR112012026205A2 (pt) 2010-04-14 2015-11-03 Strategic Enzyme Applic Inc processo para produção enzimática de um produto de fosfinotricina ou um precursor do mesmo
CN116574750B (zh) * 2023-04-21 2023-12-05 大连理工大学 一种提高腈类化合物生物转化效率的腈水合酶重组质粒及其构建方法与应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN85107055A (zh) * 1984-10-01 1986-06-10 诺沃工业联合股票公司 酶促工艺方法
JP2907479B2 (ja) * 1990-02-28 1999-06-21 輝彦 別府 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体及びアミド類の製造法
EP0502476B1 (fr) * 1991-03-04 2001-07-18 Mitsubishi Rayon Co., Ltd. Vecteurs plasmidiques hybrides contenant des gènes codant pour des enzymes qui dégradent des nitriles et procédés por la préparation d'amides et d'acides

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006062189A1 (fr) * 2004-12-09 2006-06-15 Asahi Kasei Kabushiki Kaisha Transformant exprimant la nitrile hydratase

Also Published As

Publication number Publication date
WO1995004828A1 (fr) 1995-02-16
FR2708936B1 (fr) 1995-11-10
EP0665889A1 (fr) 1995-08-09
CA2146656A1 (fr) 1995-02-16
FR2708936A1 (fr) 1995-02-17

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