JPH0733637A - Skin cosmetic - Google Patents

Abstract

PURPOSE:To obtain a skin cosmetic excellent in preventing effects on skin (improving effects on roughened skin and a horny substance and accelerating effects on the turnover of a horny layer, skin beautifying effects, etc.). CONSTITUTION:This skin cosmetic is obtained by blending an epidermal growth factor with at least one selected from ceramides, cerebrosides, cholesterol and phospholipids.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to an effect of improving aged skin (effect of improving rough skin, effect of improving keratin, turnover of horny layer).
The present invention relates to a skin cosmetic having an excellent effect of speeding up the skin, a beautiful skin effect, etc.).

[0002]

BACKGROUND OF THE INVENTION Aged skin is dry and non-smooth rough skin in which exfoliation of keratinocytes is observed and the connective tissue has a high collagen / elastin ratio and wrinkles. On the other hand, in aged skin, the turnover of the stratum corneum is slow due to a decrease in cell metabolism. In order to improve it, it is generally said that the turnover becomes faster when an antiaging component is added to the skin, and various skin tissue activating components have been studied. Among them, epidermal growth factor (Eidermal G)
(rowth Factor, hereinafter abbreviated as EGF))
Is known to promote differentiation of epidermal cells, prolong cell life, and promote proliferation of skin fibroblasts by experiments using cultured cells.

Recently, as a cosmetic having an effect of preventing skin aging, a cosmetic or a dermatological agent containing EGF has been proposed (JP-A-60-258105 and JP-A-61).
-5006). However, it was revealed that sufficient effects could not be obtained when these were examined in detail. Practically, it was difficult to obtain an excellent skin cosmetic which is effective in preventing aging of the skin by repairing or improving the tissue function of the skin and thereby restoring the function originally possessed by the skin. .

[0004]

Therefore, the inventor of the present invention
As a result of intensive studies in view of the above circumstances, it is shown that the skin cosmetics described below are excellent in the effect of improving aging skin (effect of improving rough skin, effect of improving keratin, effect of accelerating turnover of horny layer, effect of beautiful skin, etc.). After confirmation, the present invention was completed.

That is, the object of the present invention is to
It is an object of the present invention to provide a skin cosmetic excellent in the effect of preventing skin aging (effect of improving rough skin, effect of improving keratin, effect of accelerating turnover of stratum corneum, effect of beautiful skin, etc.).

[0006]

The above-mentioned object is to achieve the EGF.
And at least one selected from ceramide, cerebroside, cholesterol and phospholipid.

The EGF used in the present invention can be obtained by extraction and purification from tissues derived from animals such as humans and mice, milk, saliva, blood, urine or the like, or from fermentation products of microorganisms such as Escherichia coli. It is a kind of peptide and is disclosed in the above-mentioned JP-A-60-258105 and JP-A-61-50.
It is obtained by the method disclosed in Japanese Patent Laid-Open No. 06.

Ceramide, cerebroside, cholesterol and phospholipid (intercorneal lipid) are known to exist between cells of the stratum corneum of the epidermis and show a water retaining effect. These are obtained by extraction and purification from animal-derived tissues, milk, blood or the like, or by artificial chemical synthesis.
Examples of phospholipids include diacylphosphatidylcholine, diacylphosphatidylethanolamine, diacylphosphatidylinositol, diacylphosphatidylserine and their lyso forms.

A skin cosmetic composition comprising the EGF of the present invention and at least one selected from ceramide, cerebroside, cholesterol and phospholipid has a prominent effect on the proliferation and differentiation of epidermal cells due to their synergistic effect. Promotes and enhances skin function. Further, the water-retaining effect of the stratum corneum was remarkably improved, and the function originally possessed by the skin was restored or improved to maintain the skin in a healthy state, and particularly when applied to aged skin, a remarkable effect was observed. Their action is
It was not expected from those alone.

The EGF content is preferably 0.0001 to 1.0% by weight, and the intercorneocyte lipid content is preferably 0.01 to 5.0% by weight, based on the total amount of the skin cosmetic.
If the compounding amount is less than the lower limit of each of these, the effect aimed at by the present invention is small, while if it exceeds the upper limit, it is difficult to obtain the effect corresponding to the increase.

The skin cosmetic of the present invention can be applied to lotions, emulsions, creams, packs and the like. In addition to the above, the skin cosmetic of the present invention contains a pigment,
Fragrances, preservatives, surfactants, pigments, antioxidants and the like can be appropriately added within a range that achieves the object of the present invention.

[0012]

EXAMPLES The present invention will be described in detail below based on examples and comparative examples.

Examples 1 to 5 and Comparative Examples 1 to 6 The test methods of the rough skin effect test, the keratin improvement effect test, the turnover measurement test of the stratum corneum, and the sensory test (beautiful skin effect test) will be described.

Rough skin improvement effect test A sample was applied continuously to 10 middle-aged and elderly subjects who have rough skin on the lower legs for 4 weeks, and the effect was examined. That is, about 0.5 per once a day on the test site on the left lower leg of the subject.
g of the sample was applied, and the condition of the skin before and after the start of the test was evaluated according to the criteria shown in Table 1. The lower right leg was not coated with the sample and served as a control.

[0015]

[Table 1]

The judgment results of the test site before and after the test and the control site are compared, and when the skin dryness is improved by two stages or more (for example, + →-, ++ → ±), it is effective when one stage is improved. Valid, invalid when there was no change. The test results were shown by the number of subjects who were valid or slightly valid.

Test for effect of improving keratin (increasing anti-peeling property of keratinocytes) Scotch tape (Nichiban Mending Tape) on the skin of the test area before and after the start of the test for improving rough skin described above.
Was inspected and the state of keratinocytes attached to the tape when peeled off was examined in detail by a scanning electron microscope, and the anti-peelability of skin keratinocytes was analyzed according to the criteria in Table 2 to determine the keratin improving effect. .

[0018]

[Table 2]

The evaluation was made effective when the difference between the evaluation point of the test site and that of the control site after continuous application for 4 weeks was improved by 2 points or more, 1 case was slightly effective, and 0 point was invalid. . The judgment results are shown by the number of subjects who were valid and slightly valid.

Turnover measurement test of the stratum corneum An ointment was prepared by mixing 5% by weight of the fluorescent dye dansyl chloride in white petrolatum, and applied on the skin of the forearm of 10 test subjects for 24 hours, and keratinized. Permeabilize the layers with dansyl chloride. After that, on the same site twice a day (morning
Evening) Approximately 0.2 g of the test sample was applied, and once a day, the fluorescence of dansyl chloride was examined by using an ultraviolet A lamp in the dark, and the number of days until the fluorescence disappeared was measured as the number of days until the fluorescence disappeared.
It was overloaded.

The measurement results are shown by the average value of the number of days of each subject. Normal turnover of stratum corneum is 12-
Although it is 15 days, it spreads around 18 days in aged skin. On the other hand, when the anti-aging effect appears, it is shortened to about 12 days.

Sensory test (Beautiful skin effect test) Female subjects who complain of rough skin, fine wrinkles, dry skin (35-35)
The sample was applied to 10 people (55 years old) twice a day (morning and evening) for 3 consecutive months, and the effect after 3 months was evaluated. The test results are shown by the number of people who responded that moisturized skin, smoothed skin, and tense skin were generated for each item of wettability, smoothness, and elasticity of the skin.

[Skin lotion] EGF, ceramide, cerebroside, cholesterol and phospholipid were added to the skin lotion base having the composition shown in Table 3 as shown in Table 4 to obtain each skin lotion. It prepared and the said various tests were implemented.

Composition

[Table 3]

Preparation method Component C EGF is blended with component B, and component D ceramide, cerebroside, cholesterol and phospholipid are blended with component A, and components A and B are uniformly dissolved and then component A and component B are mixed. The ingredients are mixed and dispersed by stirring and then filled in a container. At the time of use, the contents should be evenly dispersed by shaking.

Properties The results of various tests of each skin lotion are shown in Table 4. The skin cosmetics of the present invention showed good results in various tests as compared with the skin lotions of Comparative Examples 1 to 6 in which the EGF and interkeratinous lipids were not blended or only one of them was blended. Admitted. It was clear that the combination of EGF and keratinocyte lipid had a significantly superior action to promote skin function.

[0027]

[Table 4]

Examples 6 to 10 and Comparative Examples 7 to 12 [Skin Cream] Similar to Examples 1 to 5, each skin cream was prepared with the composition shown in Table 5 and the results of various tests were conducted. The results are shown in Table 6.

[0029]

[Table 5]

Preparation method Component C EGF is blended with component B, and component D ceramide, cerebroside, cholesterol and phospholipid are blended with component A, and components A and B are dissolved by heating at 80 ° C. and then mixed. While stirring, the mixture was cooled to 30 ° C. to prepare each skin cream.

Properties As shown in Table 6, Example 6 which is the skin cosmetic of the present invention
It is clear that the skin creams of Nos. 10 to 10 are excellent in all of the characteristics as compared with Comparative Examples 6 to 12.

[0032]

[Table 6]

[0033]

Industrial Applicability As described above, the present invention is a skin makeup excellent in the effect of preventing skin aging (effect of improving rough skin, effect of improving keratin, effect of accelerating turnover of horny layer, beautiful skin effect, etc.). Fees can be provided.

Claims (1)

[Claims] 1. A skin cosmetic comprising an epidermal growth factor and at least one selected from ceramide, cerebroside, cholesterol and phospholipid.