JPH07252158A - Antiallergic agent from rice - Google Patents

Abstract

(57) [Summary] [Objective] To provide an antiallergic agent which is safe and inexpensive, has stable supply of raw materials, is easy to process, and is completely safe even if it is used regularly for a long period of time. [Structure] Smashed germinated rice, rice or germinated rice extract, rice or germinated rice hydrolyzed with enzymatic decomposition or koji, rice or germinated rice are extracted Prior to the extraction, at the same time as or after the extraction, those subjected to enzymatic decomposition or koji, the extract of rice or sprouted rice or those subjected to enzymatic decomposition or koji, were subjected to alcohol fermentation or organic acid fermentation. Antiallergic agent from rice that has been performed, or each of them as they are, or containing them.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an antiallergic agent obtained from rice or sprouted rice as a raw material.

[0002]

2. Description of the Related Art At present, rice has been developed as a staple food, as well as sake, shochu, mirin, vinegar, koji, etc., and has been indispensable for life since ancient times. In addition to this, bran bags are known for cosmetic purposes. It is believed that these were due to the fact that rice was viewed as merely a staple food, or at best as a source of starch. Also, even if the bran bag is said to be good for the skin, it has been customarily used as it is, and there was no concept of an active ingredient, nor was there any idea of utilizing that active ingredient.

On the other hand, an increasing number of people are suffering from allergies that damage the body due to qualitative and quantitative changes in the immune response, resulting in abnormal regulatory function, especially excessive immune response. Various treatments have been attempted for this allergy, but the drug has side effects due to administration, restrictions on the amount used, and the period of use, which is also a problem from the viewpoint of safety caused by long-term administration. , There is a remarkable treatment only to avoid contact with the antigen that causes allergies. In other words, the present situation is that an antiallergic agent that is effective against allergies and has no side effects and is safe has not yet been developed.

[0004]

At present, side effects of drugs on the human body have become a problem, and there is a demand for antiallergic agents that have no side effects and are sufficiently safe even if they are used regularly for a long period of time. . It is an object of the present invention to provide an antiallergic agent from rice that is safe and inexpensive, has a stable supply of raw materials, is easy to process, and is completely safe even after long-term regular use. It is a thing.

[0005]

[Means for Solving the Problems] From the viewpoint of animal and plant harmony, the present inventors have conducted research on various plant components centering on rice, which is a staple food. In the process, it has become clear that rice has a number of potential and benefits that were previously unpredictable. Therefore, we picked up rice, which is used as a staple food and proved to have the highest safety, as the theme,
I have conducted comprehensive utilization research on rice. As one of the themes, we have conducted intensive research on antiallergic agents from rice, and in the process, rice and germinated rice contain ingredients that have antiallergic effects. This has led to the completion of the present invention.

In the present invention, the components having an antiallergic effect contained in rice and germinated rice have not yet been elucidated, but rice and germinated rice are
Those treated as described below were found to exhibit anti-allergic effects. Sprouted crushed rice as it is or containing it. Rice or germinated rice extract as it is or containing it.

[0007] A product obtained by enzymatically decomposing or hydrolyzing rice and germinated rice water as it is, or containing it. When extracting rice or sprouted rice, the one that has been subjected to enzymatic decomposition or koji before or at the same time as or after the extraction is used as it is or containing it. An extract of rice or germinated rice or a product of enzymatic decomposition or koji which has been subjected to alcohol fermentation or organic acid fermentation as it is or containing it. The term "allergy" as used herein refers to all diseases caused by allergy such as allergic gastritis, allergic conjunctivitis, allergic contact dermatitis, allergic constitution and allergic rhinitis.

The rice used in the present invention means japonica,
Regardless of indica rice, it refers to non-glutinous rice and brown rice such as sticky rice and white rice, regardless of variety and type. Further, 92% or more of red rice bran or 92% or less of white rice bran, which appears during whitening, may be used, which is inexpensive and economical. Also,
Germinated rice is used. Since the active ingredient is stable against heat and light, the above raw materials are dipping, steaming, roasting (all sand roasting, net roasting, hot air roasting, etc.),
Raw material treatment such as steam roasting, surface modification such as freeze-drying, photo-modification such as UV irradiation, pressure roasting such as Patrice, and frying may be performed, and the effect was not changed.

Although the rice and germinated rice are effective as they are, they are preferably crushed for practical use. In order to pulverize the rice and the sprouted rice into powder, a pulverizer or a rice mill may be used by a general method. When sprouting rice, germinated rice is soaked in water or sprayed with water to germinate. The temperature for germination is 5 to 70 ° C. However, the temperature and time do not matter as long as they germinate. Also, if there is a risk of water spoiling during germination, replace the water so that it does not decay, or
It is preferable to carry out some preservatives. Here, germination means
It refers to everything from just before germination to germinated ones. The germinated rice is washed well before use. At this time, it may be dried before use.

When extracting rice or germinated rice, or subjecting it to enzymatic decomposition or koji, the raw material rice is pulverized into granules or powders, thereby increasing the surface area and improving efficiency. You don't have to grind, but in this case,
It takes a long time to decompose and extract the rice tissue.

When extracting rice or sprouted rice with water,
High extraction temperature is efficient, but extraction can be sufficiently performed even at low temperature. However, at a low temperature of 40 ° C. or lower, it is desirable to make the pH acidic or alkaline, or add a preservative or alcohol to treat the rice so that it does not spoil. The extraction time may be long or short as long as the effective component can be extracted, and may be determined by the extraction temperature and the extraction time. The extraction may be carried out under pressure, at normal pressure, or under reduced pressure.

[0012] In the case of water extraction, the gelatinization phenomenon is the most problematic. If it becomes pasty, not only the extraction efficiency will deteriorate, but it will be extremely difficult in actual work. In order to prevent this, the reaction may be carried out by adding amylase or acidification may be carried out with hydrochloric acid or the like to cut the starch. By using this method, it can be sufficiently solved and there is no problem in practice.

Since the active ingredient in the extract is stable to acid and alkali, it is also effective to carry out acid decomposition extraction or alkali decomposition extraction. In this case, neutralize if necessary,
Desalt. Also when extracting with an organic solvent, it is desirable to pulverize or pulverize rice as much as possible before extracting. The organic solvent may be a general organic solvent such as alcohol, acetone, n-hexane, methanol, etc., but if it is harmful to the human body, it is necessary to completely remove the solvent after extraction, so a safe one is preferable.

Further, rice or germinated rice may be enzymatically decomposed or koji may be allowed to act thereon. The term "enzymatic degradation" as used herein refers to all enzymes that act on rice, such as starch degrading enzymes, proteolytic enzymes, lipolytic enzymes, fiber degrading enzymes, lignin degrading enzymes, pectin degrading enzymes, and one or more of these are allowed to act. Say that. The koji may be any type of koji mold or rice variety and type. Furthermore, in carrying out the above-mentioned extraction, the above-mentioned enzymatic decomposition and koji may be allowed to act before, simultaneously with or after the extraction.

In the present invention, it is effective to carry out the organic acid fermentation such as alcohol fermentation, lactic acid fermentation or acetic acid fermentation at the same time as or after the above-mentioned treatment, in the following points. First, if alcohol fermentation is carried out, not only there is no stickiness at the time of application, but also the concentration is easy and the active ingredient can be easily concentrated. Further, when lactic acid fermentation is used for applications such as beverages, the product of the present invention can be used as a seasoning liquid application of vinegar for improving flavor, and acetic acid fermentation can be used as a wide range of applications by organic acid fermentation. You can

Further, when the red bran portion of 92% or more was examined, it was found to be effective but weak. The product of the present invention obtained as described above is used as it is without separating the residue, or after being pressed and filtered. When it is used as it is, it is sterilized or sterilized to obtain a product. If necessary, aeration fermentation with yeast, alcohol precipitation,
You may remove sugar with a synthetic adsorbent. Further, when the product of the present invention is blended, depending on the actual application, creams, facial cleansers, milky lotions, lotions, cleansing, packs, cosmetics such as soaps, ointments, pasta agents, lotions, etc. Formulations such as tinctures, relief agents, jellies, aerosols and other external medicines. The other compounding ingredients may be any of those usually used, and further, other medicinal agents may be used in combination.

The results of examining the antiallergic action of the product of the present invention are described below. (1) Histamine release-inhibiting effect After bleeding and death of male Wistar rats,
iologicalsalt solution (N
aCl 154 mM, KCl 2.7 mM CaCl ₂
0.9 mM, HEPES 5 mM pH 7.4) 10
ml was injected intraperitoneally. After lightly pine surgering the abdomen for 90 seconds, the abdomen was opened and an abdominal cell suspension was collected. Four
The pellet of cells obtained by centrifugation at 5000 rpm for 5 minutes at 5,000 ° C was resuspended in a solution prepared by adding 1 g / 1 liter of Dulcose to the above-mentioned saline solution.

The present invention was added to 50 μl of the isolated mast cell suspension.
After adding 75 ml and incubating at 37 ° C. for 15 minutes, 48/80 solution (maximum concentration 10 ⁻⁷ g / ml) 20
0 μl was added, and the cells were further incubated for 10 minutes. The reaction was stopped by cooling with ice, and the histamine content remaining in the centrifuged supernatant and cells was fluorimetrically determined to calculate the histamine release rate. The value measured in the same manner without adding the product of the present invention was used as a control. The results are shown in Table 1.

[0019]

[Table 1]

As shown in Table 1, it can be said that the product of the present invention suppresses the release of histamine, which is one of the causes of allergic reaction. (2) PCA reaction The back hair of a male rat weighing about 150 g was shaved, and four (8 in total) marks were placed on the skin on both sides of the back at equal intervals with a mouse anti-ovalbumin antibody. Precise intradermal injection and passive sensitization. Approximately 3.5 hours later, the product of the present invention was intradermally injected into two symmetrical points on both sides of the back.
After 0 minutes, ovalbumin-Evans blue solution was injected into the tail vein. After 30 minutes, the animals were killed by decapitation, the skin was peeled from the neck, and the area (unit: mm ² ) of circular or elliptical blue spots formed on the inner surface of the skin was determined. As a comparative control, an intradermal injection of physiological saline was used.
The results are shown in Table 2.

[0021]

[Table 2]

As can be seen from Table 2, the product of the present invention has P
It can be said that the CA reaction is suppressed. This suggests that the antigen-antibody reaction on mast cells suppresses the release of various chemical mediators, and further the series of reactions such as vascular permeability enhancement by the released chemical mediators. (3) Clinical test in humans First, the product of the present invention is applied to panelists of various diseases twice daily in the morning and evening.
If allergic gastritis is taken for drinking, if allergic conjunctivitis is instilled, if allergic rhinitis is sprayed, administration is performed according to each disease, and the administration is continued for 1 month, the progress is diagnosed, and the effect of the present invention is effective. Judged the sex. The results are shown in Table 3. It should be noted that the panelists were conducted by 10 or more persons for each of the present invention products and each disease. Judgment was made according to significant improvement, improvement, slight improvement, no change, discontinuation, and the usefulness rate (significant improvement + improvement + overall improvement rate). The judgment was performed by a specialist doctor.

[0023]

[Table 3]

As can be seen from Table 3, all the products of the present invention were found to have antiallergic properties against various allergies. Furthermore, although it is a slight disease, it is also effective for panelists who use antihistamines or steroids such as allergic vasculitis, allergic diarrhea, allergic neuritis, allergic arthritis, allergic laryngitis, etc. there were. As described above, the present invention has obtained an antiallergic agent having a very excellent effect from a safe rice as a variety of allergies. In addition, although the example and the data accompanying it are described for the case of brown rice, the same effect was observed for the case of white rice and white rice bran of 92% or less.

[0025]

【Example】

(Example 1) 1 kg of rice without germ was soaked in water at 25 ° C for 3 days to germinate rice. After thoroughly washing the germinated rice, it is dried at 50 ° C. for 24 hours, and then,
The product was finely pulverized to obtain 990 g of the product of the present invention. (Example 2) Brown rice was crushed to obtain a crushed brown rice product 500
g was obtained. 1500 ml of water was added to this pulverized product, the pH was lowered with hydrochloric acid, and the mixture was left for 10 days. Then, it was squeezed with a squeezing machine to neutralize the resulting clear liquid to obtain 1200 ml of the product of the present invention and 760 g of a residue.

Example 3 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 3 was carried out to obtain another 1190 ml of the product of the present invention. (Example 4) Brown rice is crushed to obtain 500 crushed brown rice.
g was obtained. Liquefaction enzyme 10g and water 1500m to this crushed material
1 was added. Then, the temperature was gradually raised, and the mixture was boiled and extracted for 5 minutes and then cooled. After that, squeeze with a wringer,
1420 ml of the product of the present invention and 560 g of a residue were obtained.

Example 5 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 4 was carried out to obtain 1400 ml of another product of the present invention. (Example 6) Brown rice is crushed by a crusher to obtain crushed brown rice 500
g was obtained. 1500 ml of 2N-NaOH was added to this pulverized product and the mixture was left for 5 days. Then, it was squeezed with a squeezing machine to obtain 1350 ml of the clear liquid and 650 g of the residue. 1 of this clarified liquid
It was neutralized with 0N-HCL to obtain 1480 ml of the product of the present invention.

Example 7 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 6 was carried out to obtain another 1490 ml of the product of the present invention. (Embodiment 8) Brown rice is crushed to obtain a crushed brown rice product 500
g was obtained. 1500 ml of 95% ethanol is added to this pulverized product.
Was added and left for 5 days. After that, squeeze with a wringer,
1300 ml of clear liquid and 650 g of residue were obtained. 2000 ml of water was added to this clarified liquid and concentrated by a rotary evaporator to obtain 1500 ml of the product of the present invention.

Example 9 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 8 was carried out to obtain another 1500 ml of the product of the present invention. (Example 10) Brown rice was crushed to obtain a crushed brown rice product 50
0 g was obtained, and 300 g of koji and 1500 ml of water were added to this ground product.
Was added and the mixture was allowed to stand at 55 ° C. for 20 hours. Then, it was squeezed with a squeezing machine to obtain 1230 ml of the product of the present invention and 1000 g of a residue.

(Example 11) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 10 was carried out,
1210 ml of another product of the present invention was obtained. (Example 12) Brown rice is crushed to obtain a crushed brown rice product 50
0 g was obtained. 2 g of protease and 150 water
0 ml was added and the mixture was left at 50 ° C. for 20 hours. Then, the product was squeezed with a squeezing machine to obtain 1310 ml of the product of the present invention and 670 g of a residue.

(Example 13) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 12 was carried out,
Another 1380 ml of the product of the present invention was obtained. (Example 14) Brown rice is crushed to obtain 50 crushed brown rice.
0 g was obtained. 2 g of lipolytic enzyme and 150 water
0 ml was added and the mixture was left at 50 ° C. for 20 hours. Then, it was squeezed with a squeezing machine to obtain 1290 ml of the product of the present invention and 680 g of a residue.

Example 15 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 14 was carried out,
Another 1360 ml of the product of the present invention was obtained. (Example 16) Brown rice is crushed to obtain a crushed brown rice product 50
0 g was obtained. 2 g of fiber-degrading enzyme and 150 water
0 ml was added and the mixture was left at 50 ° C. for 20 hours. Then, the product was squeezed with a squeezing machine to obtain 1330 ml of the product of the present invention and 650 g of a residue.

Example 17 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 16 was carried out,
Another 1370 ml of the product of the present invention was obtained. (Example 18) Brown rice was crushed to obtain a crushed brown rice product 50
0 g was obtained. 2g starch degrading enzyme and 150g water
0 ml was added and the mixture was left at 55 ° C. for 20 hours. Then, it was squeezed with a squeezing machine to obtain 1380 ml of the product of the present invention and 600 g of a residue.

Example 19 The same operation as in Example 18 was carried out using 500 g of the product of the present invention obtained in Example 1,
Another 1400 ml of the product of the present invention was obtained. (Example 20) Brown rice is crushed to obtain a crushed brown rice product 50
0 g was obtained. Add 2 g of pectin-degrading enzyme and 1 part of water to this ground product.
500 ml was added and left at 50 ° C. for 20 hours. After that, squeezing with a squeezing machine, 1320 ml of the present invention product and residue 660
g was obtained.

Example 21 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 20 was carried out,
Another 1300 ml of the product of the present invention was obtained. (Example 22) Brown rice is crushed to obtain a crushed brown rice product 50
0 g was obtained. To this ground product, 2 g of proteolytic enzyme, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme, 2 g of pectin degrading enzyme and 1500 ml of water were added, and the mixture was heated at 50 ° C. for 2 hours.
It was left for 0 hours. After that, the product of the present invention 14
20 ml and 560 g of residue were obtained.

(Example 23) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 22 was carried out,
Another 1440 ml of the product of the present invention was obtained. (Example 24) The same operation as in Example 22 was carried out to obtain 2000 g of an enzymatic decomposition product of rice. Then, the temperature was gradually raised, and the mixture was boiled and extracted for 5 minutes and then cooled. Then, it was squeezed with a squeezing machine to obtain 1400 ml of the product of the present invention and 550 g of a residue.

Example 25 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 24 was carried out,
1420 ml of another product of the present invention was obtained. (Example 26) Brown rice was crushed to obtain a crushed brown rice product 50
0 g was obtained. To this crushed product, 300 g of koji and 1500 ml of 40% ethanol were added, and the mixture was left at 55 ° C. for 48 hours. After that, squeeze with a squeezing machine and clarified liquid 1300 ml and residue 850
g was obtained. Then, 1000 ml of water was added to the clarified solution and concentrated with a rotary evaporator to obtain the product of the present invention 13
00 ml was obtained.

(Example 27) The same operation as in Example 26 was carried out using 500 g of the product of the present invention obtained in Example 1,
Another 1300 ml of the product of the present invention was obtained. (Example 28) Rice extract 20 was prepared in the same manner as in Example 4.
00g was obtained. To this extract, 2 g of proteolytic enzyme, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme, 2 g of pectin degrading enzyme were added, and the mixture was left at 50 ° C. for 24 hours. Then, it was squeezed with a squeezing machine to obtain 1400 ml of the product of the present invention and 580 g of a residue.

(Example 29) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 28 was carried out,
Another 1390 ml of the product of the present invention was obtained. (Example 30) In the same manner as in Example 24, 2000 g of an enzyme-decomposed extract of rice was obtained. Yeast was added to this enzyme-decomposed extract, and alcoholic fermentation was carried out for 16 days. Then, it was squeezed with a squeezing machine to obtain 1880 ml of the product of the present invention and 80 g of a residue.

(Example 31) The same operation as in Example 30 was carried out using 500 g of the product of the present invention obtained in Example 1,
Another 1800 ml of the product of the present invention was obtained. (Example 32) In the same manner as in Example 24, 2000 g of an enzyme-decomposed extract of rice was obtained. The enzyme-decomposed extract was sterilized by boiling, cooled to 37 ° C., 200 ml of a starter in which lactic acid bacteria had been cultured in advance was added, and the mixture was well stirred and sealed, and
Lactic acid fermentation was performed at 7 ° C for 2 days. Then, it was squeezed with a squeezing machine to obtain 1380 ml of the product of the present invention and 590 g of a residue.

(Example 33) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 32 was carried out,
Another 1400 ml of the product of the present invention was obtained. (Example 34) The product 1000 of the present invention obtained in Example 24.
80 ml of 95% ethanol was added to ml and the acetic acid fermentation was performed for 29 days. Then, it is filtered and the product of the present invention 990 m
1 was obtained.

(Example 35) The same operation as in Example 34 was carried out using 500 g of the product of the present invention obtained in Example 1,
Another 1000 ml of the product of the present invention was obtained. The products of the present invention obtained in the above examples are appropriately used depending on the intended use. Examples of the case where the products of the present invention are blended to make a lotion and a milky lotion will be described below. The formulation examples are not limited to the examples below.

Example 36 Lotion Water of the present invention obtained in Example 22 10.0% by weight Sorbitol 3.0% by weight Glycerin 5.0% by weight Purified water 76.4% by weight Allantoin 0.1% by weight Polyoxyethylene castor oil derivative 0.5% by weight Ethanol 5% by weight The above ingredients were mixed and dissolved by a conventional method to obtain a lotion.

(Example 37) Emulsion The product of the present invention obtained in Example 2 20.0% by weight Stearic acid 1.3% by weight Cetanol 0.7% by weight Beeswax 2.0% by weight Polyoxyethylene (11) Mono Oleic acid ester 1.2% by weight Glycerine monostearate 0.8% by weight Quinceseed extract (5% aqueous solution) 15.0% by weight Dipropylene glycol 5.0% by weight Ethanol 3.0% by weight Methylparaben 0.3 % By weight Fragrance 0.3% by weight Purified water 50.4% by weight

Dipropylene glycol was added to purified water,
The mixture is heated and stirred, and the temperature is maintained at 70 ° C.
A quince seed extract, a fragrance, and ingredients other than ethanol are added and stirred, and then uniformly emulsified with a homogenizer. While cooling the obtained emulsion, the rest of the emulsion was gradually added to the emulsion and cooled to room temperature to obtain an emulsion.

[0046]

INDUSTRIAL APPLICABILITY According to the present invention, an excellent antiallergic agent which is simple, completely safe, and has an antiallergic effect can be obtained by continuous oral administration or external use. Since rice has been the staple food until now, little has been developed about its manufacturing method and use in new fields other than food.
Furthermore, rice has been the staple food until now, and its safety has been well demonstrated. That is, the present invention not only finds a very excellent anti-allergic agent, but is now said to be overproduction of rice, has found a new use application, and that it is possible to increase consumption by improving the image of rice, It is extremely meaningful.

Claims (5)

[Claims] 1. An anti-allergic agent comprising a crushed product of germinated rice as it is or containing it. 2. An anti-allergic agent comprising rice or a germinated rice extract as it is or containing the same. 3. An anti-allergic agent, which is obtained by enzymatically decomposing or hydrolyzing rice hydrolyzed with hydrolyzed rice, or containing it. 4. An anti-allergic agent which is obtained by extracting rice or sprouted rice with enzymatic decomposition or koji before or after extraction, simultaneously with or after extraction, or containing the same. 5. An anti-allergic agent comprising rice or germinated rice extract or enzyme-decomposed or koji-acted, alcohol-fermented or organic acid-fermented as it is or containing it. .