JPH07123984A - Primer for detecting and measuring specific messenger rna - Google Patents

Abstract

PURPOSE:To provide new primers, having a specific base sequence, usable for detecting a specific messenger RNA such as a cytokine, beta-actin and a serotonin receptor and excellent in reliability. CONSTITUTION:These new primers have any of the total 35 kinds of sequences including a primer having a base sequence expressed by formula I and other 28 kinds, a primer having a base sequence expressed by formula II and another kind and a primer having a base sequence expressed by formula III and other 3 kinds or sequences homologous therewith and are useful for detecting and measuring a specific messenger RNA such as a cytokine, e.g. human interleukins or human interferons, beta-actin and a serotonin receptor respectively. The primers are readily obtained by synthesis with a DNA synthesizer and the specific messenger RNA can be detected and measured by using the primers according to a polymerase chain reactional(PCR) method.

Description

Detailed Description of the Invention

[0001]

The present invention relates to a specific messenger R
The present invention relates to a primer for detecting / measuring NA. The present invention is effectively used in fields such as the pharmaceutical industry.

[0002]

2. Description of the Related Art Messenger RNA (hereinafter referred to as mRNA
Reverse PCR as a method for detecting and measuring
Method (Reverse Polymerase Chain Reaction Method), Northern Blot Method (Northern Blot Method) and Sandwich Hybridization Method (Sandwich Hybridiza
tion Method) is known. The reverse PCR method uses reverse transcriptase to detect mRN contained in a biological sample.
A complementary DNA (hereinafter referred to as "cDNA") was synthesized from A, and this cDNA was analyzed by the following PCR method (Polymerase Chain).
Reaction method). Here, the PCR method includes two types of primers (sense primer and antisense primer; a primer is a DNA fragment used to start the synthesis of amplified DNA), a DNA polymerase, and a nucleotide in a sample containing cDNA. Is added, and a cycle of heating, cooling, and heat retention is repeated to amplify a specific length of DNA, and the amplified DNA is separated by electrophoresis, and then detected and measured. . The reverse PCR method and the PCR method are described in “J. Samblook et a,“ Edited by Sam Block, Molecular Cloning, Second Edition (Cold Spring Harbor Laboratory) (1989) ”.
l., Molecular Cloning 2nd ed., Cold Spring Harbor
Laboratory Press (1989), hereinafter referred to as the document "Molecular Cloning". Northern blotting is a method in which a sample containing mRNA is electrophoresed and separated by molecular weight, mRNA is transferred to a nylon membrane filter and immobilized, a labeled probe is added, and the label is detected and measured. This method is described in the document "Molecular Cloning".
In the sandwich hybridization method, a probe is immobilized on a carrier, a sample containing mRNA is added, another probe is labeled and added, and the label is detected and measured. For example, Parva et al. Reported that two adjacent fragments of DNA encoding the Bacillus subtilis α-amylase were used as probes to measure the mRNA of Bacillus subtilis α-amylase by the sandwich hybridization method ( A. Parva et al., DNA, Vol. 7, No. 2, 135
-P. 142 (1988) (Airi Palva et al., DNA, Vo
l.7, No.2, pp.135-142 (1988))).

As a primer for detecting and measuring mRNA by the reverse PCR method, D of the sequence shown by the following sequence number is used.
NA fragments are sold by Clontech. Human interleukin-1α 5'primer SEQ ID NO: 51 Human interleukin-1α 3'primer SEQ ID NO: 52 Human interleukin-1β 5'primer SEQ ID NO: 53 Human interleukin-1β 3'primer SEQ ID NO: 54 human interleukin-2 5 'Primer SEQ ID NO: 55 human interleukin-2 3'primer SEQ ID NO: 56 human interleukin-3 5'primer SEQ ID NO: 57 human interleukin-3 3'primer SEQ ID NO: 58 human interleukin-4 5'primer SEQ ID NO: 59 human Interleukin-4 3'primer SEQ ID NO: 60 human interleukin-5 5'primer SEQ ID NO: 61 human interleukin-5 3'primer SEQ ID NO: 62 human interleukin-6 5'primer SEQ ID NO: 63 Interleukin-6 3'primer SEQ ID NO: 64 human interleukin-7 5'primer SEQ ID NO: 65 human interleukin-7 3'primer SEQ ID NO: 66 human interleukin-8 5'primer SEQ ID NO: 67 human interleukin-8 3 ' Primer SEQ ID NO: 68 Human interferon-γ 5'primer SEQ ID NO: 69 Human interferon-γ 3'primer SEQ ID NO: 70 human β-actin 5'primer SEQ ID NO: 71 human β-actin 3'primer SEQ ID NO: 72

[0004]

However, using the DNA fragments having the sequences of SEQ ID NOs: 51 and 52 as primers, the m of human interleukin-1α was subjected to reverse PCR.
When trying to detect and measure RNA, it may not be detected sufficiently, and D of the sequences of SEQ ID NOs: 53 and 54 may be used.
When attempting to detect and measure human interleukin-1β mRNA by the reverse PCR method using NA fragment as a primer, the allowable range of the salt concentration of the reaction solution of the reverse PCR method and the temperature during the cooling operation are narrow, and the experimental conditions are limited. There are problems such as being done. The present invention solves the above-mentioned problems and provides a novel primer for detecting and measuring a specific mRNA, which is highly reliable.

[0005]

The present invention relates to the primers (a) to (d) below. (A) A primer for detecting / measuring a specific messenger RNA having any one of SEQ ID NOs: 1 to 35 or a sequence homologous thereto. (B) A primer for detecting / measuring a cytokine-specific messenger RNA having any one of SEQ ID NOS: 1 to 29 or a sequence homologous thereto. (C) A primer for detecting and measuring β-actin-specific messenger RNA, which has the sequence of SEQ ID NO: 30 or SEQ ID NO: 31 or a sequence homologous thereto. (D) A primer for detecting / measuring a serotonin receptor-specific messenger RNA having any one of SEQ ID NOs: 32 to 35 or a sequence homologous thereto.

The abbreviations used in the present specification have the following meanings. dATP: deoxyadenosine-5'-triphosphate (deox
yadenosine 5'-triphosphate) dCTP: deoxycytidine-5'-triphosphate (deoxyc
ytidine 5'-triphosphate) dGTP: deoxyguanosine-5'-triphosphate (deox
yguanosine 5'-triphosphate) dTTP: deoxythiamine-5'-triphosphate (deoxyt
hiamin 5'-triphosphate) dT: deoxythymidylic acid

The primers according to the present invention are SEQ ID NO: 1 to
D having any one of sequences 35 or a sequence homologous thereto
NA fragment. Here, the homologous sequences refer to SEQ ID NOS: 1 to
It is a sequence obtained by substituting uracil for at least one thymine in the base sequence of 35 base sequences. The primer of the present invention can be easily synthesized using a commercially available DNA synthesizer. The DNA synthesizer is based on Applied Biosystems.
s) Sold by the company.

The primer of the present invention can be used for detection and measurement of specific mRNA. Specific mRNAs include mRNAs for cytokines, β-actin, serotonin receptors and the like. Cytokines are physiologically active substances produced by cells such as lymphocytes, monocytes and macrophages. In the case of human, human interleukin-1α, human interleukin-1β, human interleukin-
2, human interleukin-3, human interleukin-4, human interleukin-5, human interleukin-6, human interleukin-7, human interleukin-8 or human interferon-γ. To detect and measure mRNA of these physiologically active substances,
To investigate the relationship between specific diseases and the expression status of the genes encoding these physiologically active substances, and to investigate the effect of new drugs under development on the immune system of mammals including humans at the gene expression level. It has important significance. β-actin is a muscle contractile protein that is universally contained in mammalian cells. Since the β-actin gene is always expressed, β-actin mRNA can be used as a positive control when determining whether the experimental system using mRNA is normal. Serotonin is a neurotransmitter in the brain and a substance that serves as an index of neural activity. The serotonin receptor is a protein to which this serotonin binds and is known to be closely related to neural activity. In the case of rat, there are rat serotonin 1c receptor, rat serotonin 2 receptor and the like. Detection and measurement of serotonin receptor mRNA can be used to investigate the relationship between specific diseases and the expression status of genes encoding these proteins, and the effects of new drugs under development on the nervous system of mammals including humans. It has important significance in investigating the gene expression level.

Using the primers of the present invention, a specific mRN
In order to detect and measure A, cDNA is synthesized from mRNA by oligo (dT) and reverse transcriptase, the primer of the present invention and DNA polymerase are added, and the steps of cooling, keeping, and heating are repeated. Detection / measurement of specific mRNA using the primer of the present invention is usually performed by the following procedure. (A) cDNA is synthesized from mRNA. (B) Heating, DNA polymerase, dATP, dCT
A buffer solution containing P, dGTT and dTTP is added and kept warm. (C) A buffer solution containing a single-stranded nucleic acid degrading enzyme is added, kept warm, and heated. (D) Primer of the present invention, DNA polymerase, dA
A buffer solution containing TP, dCTP, dGTP and dTTP is added and heated. (E) Cool, keep warm, and heat. Repeat steps (f) and (e). (G) Detect and measure the nucleic acid contained in the reaction solution. In the step (a), the oligo (d
T), a reverse transcriptase, dATP, dCTP, dGTP, and a buffer containing dTTP are added, and it heat-retains. A lysate of cells or tissues can be used as the sample containing mRNA. To lyse cells or tissues, for example, the cells or tissues are kept warm in a buffer solution containing ethylenediaminetetraacetic acid, sodium dodecyl sulfate, an RNAse inhibitor and proteinase K. Reverse transcriptase and RNA degrading enzyme inhibitors can be purchased from Toyobo Co., Ltd., and Proteinase K can be purchased from Wako Pure Chemical Industries, Ltd. The general procedure for lysis of cells and tissues in the process (a) is described in the document "Molecular
In addition, a cDNA synthesis kit sold by Toyobo Co., Ltd. or the like may be used for carrying out the step (a).

Steps (a) and (c) are steps for converting a single-stranded cDNA into a stable double-stranded cDNA. Examples of the DNA polymerase include Klenow fragment (Klenow f
ragment) is, for example, S1 as the single-stranded nucleolytic enzyme.
Nucleace is available. Heating is 9
Let stand for 1 to 5 minutes at 0 to 100 ° C.
Let stand at 42 ° C. for 10 to 1 hour. Klenow Fragment and S1 Nucleace can be purchased from Takara Shuzo Co., Ltd.
A general procedure for converting single-stranded cDNA into double-stranded cDNA is described in the document "Molecular Cloning".
As the DNA polymerase in the step (d), for example, Taq polymerase can be used. The heating is performed, for example, at 90 ° C to 100 ° C for 1 to 10 minutes. Tack polymerase can be purchased from Toyobo Co., Ltd.

Cooling in the step (e) is, for example, 45 ° C. to 65 ° C.
Let stand for 1 to 5 minutes and keep the temperature at 70 ℃ to 80 ℃ for 3 minutes.
Let stand for 10 minutes and heat at 90 ° C to 100 ° C for 1 minute.
Let stand for ~ 5 minutes. Step (d) heating operation and step (e)
The operations of cooling, heat retention and heating can be performed using a DNA thermal cycler (registered trademark) (manufactured by Perkin-elmer Cetus). The number of times of repeating the step (f) is not particularly limited, but is repeated about 30 times. To detect and measure the nucleic acid contained in the reaction solution of the step (g), for example, the reaction solution is subjected to electrophoresis using an ethidium bromide-containing agarose gel to separate the nucleic acid contained in the reaction solution by molecular weight. , Irradiate with ultraviolet rays. In addition, after performing the steps (d) to (f) once, the primer of the present invention to be added has a different sequence, and the steps (d) to (f) are performed again, and then the step (k) is performed. You may enter.

DNA fragments having any one of SEQ ID NOS: 1 to 35, a sequence homologous thereto or a sequence complementary to any one of SEQ ID NOS: 1 to 35 are (a ') to (d') below. It can also be used as a probe for detecting and measuring the specific mRNA of. (A ′) A probe for detecting / measuring a specific mRNA, which has any of the sequences of SEQ ID NOs: 1 to 35, a sequence homologous thereto or a sequence complementary to any of the sequences of SEQ ID NOs: 1 to 35. (B ′) A probe for detecting / measuring cytokine-specific mRNA, which has a sequence of any of SEQ ID NOS: 1-29, a sequence homologous thereto or a sequence complementary to any of the sequences of SEQ ID NOS: 1-29 . (C ′) Sequence number 30 or sequence number 31, sequence homologous thereto or sequence number 30 or sequence number 31
Β-actin specific m having a sequence complementary to that of
A probe for detecting and measuring RNA. (D ′) Serotonin receptor-specific m having a sequence of any of SEQ ID NOs: 32 to 35, a sequence homologous thereto, or a sequence complementary to any sequence of SEQ ID NOs: 32 to 35
A probe for detecting and measuring RNA.

The constituent element of the probe for detecting / measuring the specific mRNA may be labeled with a radioisotope. For labeling with a radioisotope, for example, T4 polynucleotide kinase (T4 polynucleotide
(γ- ³² P) dATP is added to this in the presence of a kinase. General techniques for labeling with radioisotopes are described in the document "Molecular Cloning". T4 polynucleotide kinase was purchased from Toyobo Co., Ltd. (γ-
³² P) dATP can be purchased from Amersham. Further, the probe may have a label added thereto. Examples of the label added to the probe include biotin (Biotin), avidin (Avidin), streptavidin (Streptoavidin), and digoxigenin (Digoxig).
Chemical substances such as enin), alkaline phosphatase (Alka
line phosphatase), luciferase,
Enzymes such as peroxidase and β-galactosidase, fluorescein (Fl)
There are fluorescent substances such as uorescine). To add biotin to a probe, for example, biotinylated deoxyuridine-5′-triphosphate (de) in the presence of terminal transferase is used.
oxyuridine 5'-triphosphate) is added. Terminal transferase and biotinylated deoxyuridine-5'-triphosphate can be purchased as a kit from Boehringer Mannheim. A commercially available kit can also be used to add a labeled substance other than biotin, and such a kit can be purchased from Takara Shuzo Co., Ltd. or Toyobo Co., Ltd. In addition, the document “Molecular
Labels may be added according to the method described in "Cloning".

In order to detect and measure a specific mRNA using the above-mentioned probe, it is necessary to add a labeled probe to the immobilized mRNA, or to add an mRNA to the immobilized probe and further label it. Add the probe. To detect and measure a specific mRNA by adding a labeled probe to the immobilized mRNA, for example, a sample containing mRNA is electrophoresed and separated by molecular weight, and the mRNA is transferred to a nylon membrane filter and immobilized. Then, add a labeled probe to detect the label.
taking measurement. This method is called Northern blotting, and its general method is described in the document "Molecular Cloning". MRNA on immobilized probe
In order to detect and measure the specific mRNA by adding another probe to which is added and labeled, for example, the probe is immobilized on a carrier, a sample containing mRNA is added, and another probe or oligo (dT ) Is labeled and added, and the label is detected and measured. This method is called a sandwich hybridization method, and its general method is described in "J.
A. Mateus et al., Analytical Biochemistry, 169, pp. 1-25 (1988) "(JAMatthe
wset al., Analytical Biochemistry, Vol.169, p.1-25
(1988)). Since the base sequence of mRNA to be detected / measured is usually sense (base sequence existing in the chain carrying the genetic information), the probe is
Of DNA fragments having a sequence of any of SEQ ID NOs: 1 to 35, a sequence homologous thereto or a sequence complementary to any of the sequences of SEQ ID NOs: 1 to 35, a sequence usually complementary to the mRNA thereof Use what is. When detecting and measuring a specific mRNA according to the present invention, the equipment, water, etc. used are, for example, 0.1% (volume) diethylpyrocarbonate (hereinafter, referred to as DEPC).
Abbreviated) and left at 37 ° C for a whole day and night, and then subjected to high-pressure steam sterilization (121 ° C, 1 atm).
It is desirable to suppress NA degrading enzyme activity as much as possible.

The base sequence of the specific mRNA according to the present invention will be described below. The base sequence of specific mRNA is
It is recorded in a database concerning nucleotide sequences as the nucleotide sequence of cDNA or the nucleotide sequence of genomic DNA. As such a database, there is GenBank (registered trademark). To obtain Genbank information, for example, a medium such as a CD-ROM in which Genbank information is recorded and a dedicated information search program are used to output the nucleotide sequence information. This CD-ROM
And "HIBIO" as a dedicated information retrieval program
(Registered trademark) (manufactured by Hitachi Software Engineering Co., Ltd.), which can be purchased from Takara Shuzo Co., Ltd. Human interleukin-1α, human interleukin-1
β, human interleukin-2, human interleukin-3, human interleukin-4, human interleukin-5, human interleukin-6, human interleukin-7, human interleukin-8, human interferon-γ, human β-actin, rat β-actin,
The nucleotide sequences of the mRNAs of mouse β-actin, rat serotonin 1c receptor and rat serotonin 2 receptor are shown in GenBank CD-ROM (R 74.0).
Then you can get each from the following files. The sequences shown in these files are the sequences with the sequence numbers shown on the right side of the file names below.

Human interleukin-1α HUMIL1ALPH SEQ ID NO: 36 Human interleukin-1β HUMIL1B SEQ ID NO: 37 Human interleukin-2 HUMIL2 SEQ ID NO: 38 Human interleukin-3 HUMIL3A SEQ ID NO: 39 Human interleukin-4 HUMIL4 SEQ ID NO: 40 Human interleukin-4 Leukin-5 HUMIL5 SEQ ID NO: 41 Human interleukin-6 HUMILFNB2 SEQ ID NO: 42 Human interleukin-7 HUMIL7A SEQ ID NO: 43 Human interleukin-8 HUMBTLP SEQ ID NO: 44 Human interferon-γ HUMIFGNG SEQ ID NO: 45 Human β-actin HSAC07 SEQ ID NO: 46 Rat β-actin RNAC01 SEQ ID NO: 47 Mouse β-actin MMACTBR SEQ ID NO: 48 Rat Serotonin 1c Receptor RATSR1CA SEQ ID NO: 49 Rat Serotonin 2 Receptor RN5HT2 SEQ ID NO: 50

Next, the features of the sequences of SEQ ID NOS: 1 to 35 will be described in association with the base sequence of specific mRNA.
The sequences of SEQ ID NOs: 1, 2 and 3 are HUMIL, respectively.
It is a sequence complementary to the 100th to 122nd sequence, the 212nd to 234th sequence, and the 452nd to 476th sequence of the 1ALPH base sequence. HUM
The base sequence of IL1ALPH is human interleukin-
Of the double-stranded cDNA of 1α, this is the base sequence of the strand having the genetic information (sense strand). Therefore SEQ ID NO: 1
The DNA fragments of sequences 2 and 2 can both be used as sense primers when detecting and measuring human interleukin-1α mRNA by the reverse PCR method, and the DNA fragment of sequence SEQ ID NO: 3 can be used as an antisense primer. (The sense primer is a primer used to start the synthesis of the strand having the genetic information in the amplified DNA, and the antisense primer is used to start the synthesis of the complementary strand thereof.). Furthermore, the DNA of the sequence of SEQ ID NO: 1, 2 or 3 or its complementary sequence
The fragment can also be used as a probe when detecting and measuring human interleukin-1α mRNA by Northern blotting or sandwich hybridization. Using the DNA fragments of the sequences of SEQ ID NOs: 1 and 3 as primers, this mRNA was detected by the reverse PCR method.
When measured, the size of the amplified DNA is about 370 base pairs (bp), and the size of the DNA fragments of SEQ ID NOs: 2 and 3 is about 260 bp.

The sequences of SEQ ID NOs: 4, 5 and 6 are 3617 to 36 of the base sequence of HUMIL1B, respectively.
The sequences complementary to the 36th sequence, the 4370th to 4390th sequences, and the 5817th to 5838th sequences. The base sequence of HUMIL1B is the base sequence of human interleukin-1β genomic DNA, and the sequences of SEQ ID NOs: 4, 5 and 6 are all HUMIL.
It relates to the exon portion of the base sequence of 1B. Therefore, the DNA fragments having the sequences of SEQ ID NOS: 4 and 5 can be used as sense primers when detecting and measuring the mRNA of human interleukin-1β by the reverse PCR method, and the DNA fragments having the sequence of SEQ ID NO: 6 are antisense. Can be used as a primer. Furthermore, the DNA of the sequence of SEQ ID NO: 4, 5 or 6 or its complementary sequence
The fragment can also be used as a probe when detecting and measuring human interleukin-1β mRNA by Northern blotting or sandwich hybridization. Using the DNA fragments of the sequences of SEQ ID NOs: 4 and 6 as primers, this mRNA was detected by the reverse PCR method.
When measured, the size of amplified DNA is about 440bp
In the case of the DNA fragments having the sequences of SEQ ID NOs: 5 and 6, the length is about 230 bp. It should be noted that among the nucleotide sequences of HUMIL1B, 446th to 908th and 971th to 1st
535th, 1588th to 3575th, 3778th
The 4322nd, 4488th to 5722nd and 5854th to 6569th sequences are introns.

The sequences of SEQ ID NOs: 7, 8, 9 and 10 are respectively 582 to 60 of the base sequence of HUMIL2.
6th array, 744th to 768th arrays, 32
02nd to 3211th and 5057th to 5070
The sequence complementary to the th sequence, and 5139 to 51
The sequence is complementary to the 61st sequence. HUMIL2
The nucleotide sequence of is the genomic D of human interleukin-2.
The nucleotide sequences of NA, and the sequences of SEQ ID NOS: 7, 8, 9 and 10 all relate to the exon portion of the nucleotide sequence of HUMIL2. Therefore, the DNA fragments having the sequences of SEQ ID NOs: 7 and 8 are both human interleukin-2.
Can be used as a sense primer in the case of detecting / measuring the mRNA of Escherichia coli by the reverse PCR method.
The DNA fragment having the sequence of can be used as an antisense primer. Furthermore, the DNA fragment of the sequence of SEQ ID NO: 7, 8, 9 or 10 or its complementary sequence may also be used as a probe when detecting and measuring human interleukin-2 mRNA by Northern blotting or sandwich hybridization. Available. SEQ ID NOS: 7 and 10
Using the DNA fragment of the sequence of
When NA is detected and measured by the reverse PCR method, the size of the amplified DNA is about 350 bp, and the size of the DNA fragments of SEQ ID NOs: 8 and 9 is about 190 bp.
In the base sequence of HUMIL2, 625th to 714th, 775th to 3067th and 3212th
The sequence from the 50th position to the 5056th position is an intron.

The sequences of SEQ ID NOs: 11 and 12 are 163rd to 163rd of the base sequence of HUMIL3A, respectively.
The second sequence and the sequence complementary to the 441st to 462nd sequences. The base sequence of HUMIL3A is the base sequence of the sense strand in the double-stranded cDNA of human interleukin-3. Therefore, the DNA fragment having the sequence of SEQ ID NO: 11 is the mRNA of human interleukin-3.
Can be used as a sense primer for detection / measurement by the reverse PCR method, and the DNA fragment having the sequence of SEQ ID NO: 12 can be used as an antisense primer. Furthermore, the DNA fragment of the sequence of SEQ ID NO: 11 or 12 or its complementary sequence is the human interleukin-3 mRNA.
It can also be used as a probe when detecting and measuring NA by Northern blotting or sandwich hybridization. Using the DNA fragments of the sequences of SEQ ID NOs: 11 and 12 as a primer,
When detected and measured by the R method, the size of the amplified DNA is about 320 bp.

The sequences of SEQ ID NOs: 13, 14 and 15 are from 139th to 16th of the base sequence of HUMIL4, respectively.
It is a sequence complementary to the 0th sequence, the 210th to 231st sequences, and the 480th to 501st sequences. The base sequence of HUMIL4 is the base sequence of the sense strand in the double-stranded cDNA of human interleukin-4. Therefore, the DNA fragments having the sequences of SEQ ID NOs: 13 and 14 can both be used as sense primers when detecting and measuring the mRNA of human interleukin-4 by the reverse PCR method, and the DNA fragment having the sequence of SEQ ID NO: 15 is antisense. Can be used as a primer. further,
The DNA fragment having the sequence of SEQ ID NO: 13, 14 or 15 or its complementary sequence is the human interleukin-4 m fragment.
It can also be used as a probe when detecting and measuring RNA by Northern blotting or sandwich hybridization. Using the DNA fragments of the sequences of SEQ ID NOs: 13 and 15 as primers, this mRNA was labeled with reverse P
When detected and measured by the CR method, the amplified DNA has a size of about 360 bp, and the DNA fragments having the sequences of SEQ ID NOs: 14 and 15 have a size of about 290 bp.

The sequences of SEQ ID NOs: 16 and 17 are sequences complementary to the 619th to 640th sequences and the 2139th to 2160th sequences of the base sequence of HUMIL5, respectively. The base sequence of HUMIL5 is the base sequence of human interleukin-5 genomic DNA, and the sequences of SEQ ID NOs: 16 and 17 are both HUMI.
It relates to the exon part of the base sequence of L5. Therefore, the DNA fragment having the sequence of SEQ ID NO: 16 can be used as a sense primer when detecting and measuring human interleukin-5 mRNA by the reverse PCR method, and the DNA fragment having the sequence of SEQ ID NO: 17 can be used as an antisense primer. . Furthermore, SEQ ID NO: 16 or 1
The DNA fragment of sequence 7 or its complementary sequence can also be used as a probe when detecting and measuring human interleukin-5 mRNA by Northern blotting or sandwich hybridization. When the DNA fragments of the sequences of SEQ ID NOs: 16 and 17 are used as primers and this mRNA is detected and measured by the reverse PCR method, the size of the amplified DNA is about 280 bp. In addition,
From the 697th position to the 904th position of the base sequence of HUMIL5
The sequences 938, 1882 to 1882 and 2012 to 2117 are introns.

The sequences of SEQ ID NOs: 18, 19, 20 and 21 are complementary to the 225th to 247th sequences, the 282nd to 305th sequences, and the 453rd to 475th sequences of the base sequence of HUMIFNB2, respectively. The sequence and a sequence complementary to the 630th to 651st sequences. The base sequence of HUMIFNB2 is the base sequence of the sense strand in the double-stranded cDNA of human interleukin-6. Therefore, the DNA fragments having the sequences of SEQ ID NOs: 18 and 19 are both human interleukin-6.
Can be used as a sense primer in the case of detecting / measuring the mRNA of Escherichia coli by the reverse PCR method.
The DNA fragment of sequence 1 can be used as an antisense primer. Furthermore, the DNA fragment of the sequence of SEQ ID NO: 18, 19, 20 or 21 or its complementary sequence can also be used as a probe for detecting and measuring human interleukin-6 mRNA by Northern blotting or sandwich hybridization. Available. Sequence number 1
When the DNA fragments of the sequences 8 and 21 were used as primers and this mRNA was detected and measured by the reverse PCR method, the size of the amplified DNA was about 430 bp,
About 1 in the case of the DNA fragments having the sequences of SEQ ID NOs: 19 and 20
It becomes 90 bp.

The sequences of SEQ ID NOs: 22, 23 and 24 are 498th to 5th base sequences of HUMIL7A, respectively.
It is a sequence complementary to the 20th sequence, the 616th to 638th sequences, and the 897th to 918th sequences. The base sequence of HUMIL7A is the base sequence of the sense strand in the double-stranded cDNA of human interleukin-7. Therefore, the DN of the sequences of SEQ ID NOs: 22 and 23
All of the A fragments are human interleukin-7 mRN.
A DNA having the sequence of SEQ ID NO: 24, which can be used as a sense primer when detecting and measuring A by the reverse PCR method.
The fragment can be used as an antisense primer. Furthermore, the DNA fragment of the sequence of SEQ ID NO: 22, 23 or 24 or its complementary sequence is human interleukin-7.
Can also be used as a probe when detecting / measuring the above mRNA by Northern blotting or sandwich hybridization. DN of sequences SEQ ID NOs: 22 and 24
When the A fragment is used as a primer and this mRNA is detected and measured by the reverse PCR method, the size of the amplified DNA is about 420 bp, and that of the DNA fragments having the sequences of SEQ ID NOs: 23 and 24 is about 300 bp.

The sequences of SEQ ID NOs: 25 and 26 are respectively 146th to 167th of the base sequence of HUMBTLP.
Th sequence and a sequence complementary to the 355th to 376th sequences. The base sequence of HUMBTLP is the base sequence of the sense strand in the double-stranded cDNA of human interleukin-8. Therefore, the DNA fragment of the sequence of SEQ ID NO: 25 is a human interleukin-8 mRNA.
Can be used as a sense primer for detection and measurement by reverse PCR, and the DNA fragment having the sequence of SEQ ID NO: 26 can be used as an antisense primer. Furthermore, the DNA fragment of the sequence of SEQ ID NO: 25 or 26 or its complementary sequence is the human interleukin-8 mR.
It can also be used as a probe when detecting and measuring NA by Northern blotting or sandwich hybridization. Using the DNA fragments of the sequences of SEQ ID NOs: 25 and 26 as a primer,
When detected and measured by the R method, the size of the amplified DNA is about 230 bp.

The sequences of SEQ ID NOs: 27, 28 and 29 are respectively from 502nd to 5th of the base sequence of HUMIFNG.
The sequence complementary to the 23rd sequence, the 1834th to 1855th sequences, and the 2138th to 2160th sequences. The base sequence of HUMIFNG is the base sequence of genomic DNA of human interferon-γ, and the sequences of SEQ ID NOs: 27, 28 and 29 are all HU.
It relates to the exon portion of the base sequence of MIFNG. Therefore, the DNA fragments having the sequences of SEQ ID NOs: 27 and 28 can be used as sense primers when detecting and measuring human interferon-γ mRNA by the reverse PCR method, and the DNA fragments having the sequence of SEQ ID NO: 29 can be used as an antisense primer. it can. Furthermore, the DNA fragment of the sequence of SEQ ID NO: 27, 28 or 29 or its complementary sequence can also be used as a probe when detecting and measuring human interferon-γ mRNA by Northern blotting or sandwich hybridization. When the DNA fragments of SEQ ID NOS: 27 and 29 were used as primers and this mRNA was detected and measured by the reverse PCR method, the size of the amplified DNA was about 32.
It becomes 0 bp, and in the case of the DNA fragments having the sequences of SEQ ID NOs: 28 and 29, it becomes about 230 bp. In addition, HUMIFNG
Of the nucleotide sequences of 589th to 1827th, 18th
97th to 1991th and 2175th to 459th
The 9th sequence is an intron.

The sequences of SEQ ID NOs: 30 and 31 are sequences complementary to the 101st to 118th sequences and the 422nd to 441st sequences of the base sequence of HSAC07, respectively. The base sequence of HSAC07 is human β
-The base sequence of the sense strand of the double-stranded cDNA of actin. Therefore, the DNA fragment having the sequence of SEQ ID NO: 30 can be used as a sense primer when detecting and measuring human β-actin mRNA by the reverse PCR method, and the DNA fragment having the sequence of SEQ ID NO: 31 can be used as an antisense primer. Furthermore, the DNA fragment of the sequence of SEQ ID NO: 30 or 31, or its complementary sequence is
It can also be used as a probe when detecting and measuring human β-actin mRNA by Northern blotting or sandwich hybridization. Also, SEQ ID NO: 3
The sequences 0 and 31 are the 1303rd to 1320th sequences of the base sequence of RNAC01, and 217, respectively.
It is also a sequence complementary to the 5th to 2194th sequences. The base sequence of RNAC01 is the base sequence of rat β-actin genomic DNA, and the sequences of SEQ ID NOs: 30 and 31 both relate to the exon portion of the base sequence of RNAC01. Furthermore, the sequences of SEQ ID NOs: 30 and 31 are also sequences complementary to the 140th to 157th sequences and the 461st to 480th sequences of the base sequence of MMACTBR, respectively. MMA
The base sequence of CTBR is the base sequence of the sense strand of the double-stranded cDNA of mouse β-actin. Therefore, the DNA fragment having the sequence of SEQ ID NO: 30 can be commonly used as a sense primer when detecting and measuring the mRNA of human, rat or mouse β-actin by the reverse PCR method. It can be commonly used as an antisense primer. Furthermore, D of the sequence of SEQ ID NO: 30 or 31 or its complementary sequence
The NA fragment can also be used as a common probe when detecting and measuring human, rat or mouse β-actin mRNA by Northern blotting or sandwich hybridization. Using the DNA fragments of the sequences of SEQ ID NOs: 30 and 31 as primers,
Is detected and measured by the reverse PCR method, amplified D
The size of NA is about 340 bp in each case. In addition, R
From the nucleotide sequence of NAC01, from the 309th position to the 1237th position
Th, 1367 to 1453, 1694 to 2157, 2597 to 2684, and 2
The 867th to 2990th sequences are introns.

The sequences of SEQ ID NOs: 32 and 33 are respectively from the 994th position to the 10th position of the base sequence of RASSR1CA.
It is a sequence complementary to the 13th sequence and the 1349th to 1368th sequences. The base sequence of RATSR1CA is the double-stranded c of rat serotonin 1c receptor.
It is the base sequence of the sense strand of DNA. Therefore, the DNA fragment having the sequence of SEQ ID NO: 32 can be used as a sense primer when detecting and measuring mRNA of rat serotonin 1c receptor by the reverse PCR method, and the DNA fragment having the sequence of SEQ ID NO: 33 can be used as an antisense primer. . Furthermore, SEQ ID NO: 32 or 3
The DNA fragment of sequence 3 or its complementary sequence can also be used as a probe when detecting and measuring rat serotonin 1c receptor mRNA by Northern blotting or sandwich hybridization. When the DNA fragments of the sequences of SEQ ID NOs: 32 and 33 are used as primers and this mRNA is detected and measured by the reverse PCR method, the size of the amplified DNA is about 380 bp.

The sequences of SEQ ID NOs: 34 and 35 are respectively 1115th to 113rd of the base sequence of RN5HT2.
It is a sequence complementary to the 1st sequence and the 1510th to 1526th sequences. The nucleotide sequence of RN5HT2 is a double-stranded cDNA of rat serotonin 2 receptor.
Of these, the base sequence of the sense strand. Therefore, the DNA fragment having the sequence of SEQ ID NO: 34 can be used as a sense primer when detecting and measuring mRNA of rat serotonin 2 receptor by the reverse PCR method.
The DNA fragment of sequence 5 can be used as an antisense primer. Furthermore, the DNA fragment having the sequence of SEQ ID NO: 34 or 35 or a sequence complementary thereto can also be used as a probe when detecting and measuring rat serotonin 2 receptor mRNA by Northern blotting or sandwich hybridization. When the DNA fragments of the sequences of SEQ ID NOs: 34 and 35 are used as primers and this mRNA is detected and measured by the reverse PCR method, the size of the amplified DNA is about 420 bp.

[0030]

The present invention will be described in detail below with reference to examples. Example 1 Human interleukin-1α and β (a) Measurement of mRNA of human interleukin 1α and β DNA fragments having the sequences of SEQ ID NOs: 1 to 6 were respectively subjected to DN.
A Synthesizer (Applied Biosystems)
ystems), 394 type), dried with a centrifugal evaporator (Tokyo Scientific Instruments, VE-100 type), and dissolved in water treated with DEPC to a concentration of 100 μg / ml. SEQ ID NO: 1 in 4 0.5 ml tubes
Add 1 μl of each of the DNA fragments of sequences 3 and 3,
Furthermore, 1x 10x concentrated buffer for PCR (Promega)
1, 25 mM magnesium chloride solution (Promega)
4 μl, 10 mM dNTP solution (Promega) 0.8
μl and tack polymerase (5000 unit / m
l) (Promega) 0.1 μl was added. As samples, DEPC-treated water, human interleukin 1α cDNA clone aqueous solution (50 attomole / ml) (Clontech), human monocyte cDNA library aqueous solution (1.2 × 10 ⁶ pfu / μl) (clones) Tech Co., Ltd.) or human genomic DNA aqueous solution (100 μg / m
1) (Clontech) (for specificity study) (1 μl) was added, and the total amount was adjusted to 10 μl with DEPC-treated water. Reaction solutions were similarly prepared for the DNA fragments having the sequences of SEQ ID NOs: 2 and 3. Separately, four 0.5 ml tubes were prepared, and 1 μl of each of the DNA fragments having the sequences of SEQ ID NOs: 4 and 6 was put therein, and as samples, DEPC-treated water and human interleukin 1β cDNA clone aqueous solution (50 attomole / ml ) (Clontech), human monocyte cd
A reaction solution was similarly prepared using an NA library aqueous solution or a human genomic DNA aqueous solution. SEQ ID NOs: 5 and 6
A reaction solution was prepared in the same manner for the DNA fragment having the sequence of.

One drop of mineral oil (Sigma) was added to these reaction solutions, and PCR was carried out with a DNA thermal cycler (Perkin Elmer Cetus). PCR conditions: heating at 94 ° C for 10 minutes, then at 55 ° C for 1.5 minutes, 72 ° C
A cycle of 4 minutes at 94 ° C. and 1.5 minutes at 94 ° C. was repeated 30 times. After the PCR was completed, 10 μl of the reaction solution was taken and loading buffer (0.25% Bromophenol blue, 0.25% Xylenecyanol FF), 15% Ficol type 400 (Ficol type 400) was added. 2 μl of an aqueous solution containing 5 μg / ml was added to 1.5% agarose gel containing ethidium bromide and electrophoresed at 3 V / cm. After the electrophoresis was completed, the agarose gel was irradiated with ultraviolet rays to observe bands.

FIG. 1 is a diagram showing the appearance of bands observed by the above electrophoresis. In the figure, lanes 1 and 10 are electrophoresed with molecular weight markers, and the numbers to the left of each band on lane 1 indicate the molecular weight of each band (unit is base pair (bp)). Lanes 2-5, 6-
9, 11 to 14 and 15 to 18 show electrophoresed reaction solutions when the DNA fragments having the sequences of SEQ ID NOs: 2 and 3, 1 and 3, 5 and 6, and 4 and 6 were used, respectively. Lanes 2, 6, 11 and 15 are all samples of DEPC-treated water, lanes 3 and 7 are all human interleukin 1α cDNA clone aqueous solutions, and lanes 12 and 16 are all human interleukin 1 samples.
An aqueous β cDNA clone solution was used for lanes 4, 8, 13 and 17, and an aqueous human monocyte cDNA library solution, and lanes 5, 9, 14 and 18 for human genomic DNA solution. The electrophoresed reaction solution is shown. In the figure, bands are observed at almost the same positions in lanes 3 and 4, lanes 7 and 8, lanes 12 and 13, and lanes 16 and 17, respectively.
The molecular weights of these bands are expected (about 260 bp, about 370 bp, about 230 bp and about 44 bp, respectively).
0 bp). On the other hand, lanes 2, 5, 6, 9, 1
No bands were observed at 1, 14, 15 and 18.

(B) Comparison of sensitivity between the primer of the present invention and a commercially available primer As control primers, DNA fragments having the sequences of SEQ ID NOs: 51 and 52 were used. An aqueous solution of human monocyte cDNA library was diluted with DEPC-treated water to prepare a stock solution, 1/10.
Dilution, 1/100 dilution, 1/1000 dilution, 1/100
00 dilution, 1/100000 dilution, 1 / 1,000,000
Dilution series of dilution and 1/1000000 dilution were prepared. 1 μl of each control primer was placed in 10 0.5 ml tubes, and DEPC-treated water, human interleukin 1α cDNA clone aqueous solution or the above dilution series was used as a sample, and the reaction solution was the same as in (a) above. Was prepared. Separately, 10 0.5 ml tubes were prepared separately, and 1 μl of each of the DNA fragments having the sequences of SEQ ID NOs: 1 and 3 was added, and a reaction solution was prepared in the same manner as above.
Similarly, reaction solutions were prepared for the DNA fragments having the sequences of SEQ ID NOs: 2 and 3. PCR was performed on these reaction solutions in the same manner as in (a) above, and the bands were observed by electrophoresis. With the control primer, a band was observed in the undiluted human monocyte cDNA library aqueous solution, but no band was observed in the diluted one. on the other hand,
With the DNA fragments having the sequences of SEQ ID NOs: 1 and 3, bands were observed up to 1/10000 of the human monocyte cDNA library aqueous solution, and 1/1000 with the DNA fragments having the sequences of SEQ ID NOs: 2 and 3. Bands were observed up to what was done.

(C) Comparison of PCR conditions between the primer of the present invention and a commercially available primer The DNA fragments having the sequences of SEQ ID NOs: 53 and 54 were used as a control primer. 1 μl of each control primer was placed in 6 0.5 ml tubes, and an aqueous human monocyte cDNA library solution was used as a sample.
A reaction solution was prepared in the same manner as in the above (a). However, for the magnesium chloride solution, 0.2 μl, 0.4 μl,
0.6 μl, 0.8 μl, 1.0 μl or 1.2 μ was dispensed. On the other hand, prepare 6 tubes of 0.5 ml separately,
1 μl each of the DNA fragments of SEQ ID NOs: 4 and 6
Then, the reaction solution was prepared in the same manner as above. PCR was performed on these reaction solutions in the same manner as in (a) above, and the bands were observed by electrophoresis. The heating, cooling, and heat retaining steps are the same as those in (a) above, except that the condition of "55 ° C for 1.5 minutes" is "45 ° C for 1.5 minutes".
And "1.5 minutes at 65 ° C" were also carried out. In any of the cooling steps, in the case of the primer of the present invention, the target band was observed regardless of the dispensed amount of the magnesium chloride solution. However, in the case of the control primer, when the cooling step was “1.5 minutes at 55 ° C.”, the amount of the magnesium chloride solution dispensed was 0.2 μl (that is, the concentration of magnesium ion in the reaction solution was 0.5 mM). No band was observed. When the cooling step is “1.5 minutes at 45 ° C.”, the dispensed amount of the magnesium chloride solution is 0.
Bands other than the target band were also observed when the concentration was 6 μl (that is, the magnesium ion concentration in the reaction solution was 1.5 mM). Furthermore, the cooling process is "1.5 minutes at 65 ° C"
Then, the dispensed amount of 25 mM magnesium chloride solution is 0.2
No band was observed when μl (that is, the concentration of magnesium ion in the reaction solution was 0.5 mM).

Example 2 Human interleukin-2 DNA fragments having the sequences of SEQ ID NOs: 7 to 10 were synthesized in the same manner as in Example 1. 1 μl each of the DNA fragments having the sequences of SEQ ID NOS: 7 and 10 was placed in three 0.5 ml tubes, and DEPC-treated water and a human interleukin-2 cDNA clone aqueous solution (50 attomole / m 2) were used as samples.
l) (Clontech) or an aqueous human genomic DNA solution was used to prepare a reaction solution in the same manner as in Example 1. Reaction solutions were similarly prepared for the DNA fragments having the sequences of SEQ ID NOs: 8 and 9. PCR was performed on these reaction solutions in the same manner as in Example 1, and electrophoresis was performed to observe bands. FIG. 2 is a diagram showing the appearance of bands observed by the above electrophoresis. In the figure, lane 1 shows that the molecular weight marker was electrophoresed, and the numbers on the left side of each band on this lane indicate the molecular weight of each band (unit is base pair (b
p)) is shown. Lanes 2 to 4 and 5 to 7 are DNAs of the sequences of SEQ ID NOs: 8 and 9 and 7 and 10, respectively.
The electrophoresed reaction solution when fragments were used is shown. Lanes 2 and 5, Lanes 3 and 6, and Lanes 4 and 7
Shows electrophoresed reaction solutions when DEPC-treated water, human interleukin-2 cDNA clone aqueous solution, and human genomic DNA aqueous solution were used as samples, respectively. In the figure, bands were observed in lanes 3 and 6, respectively, and the molecular weights of these bands were in agreement with expected values (about 190 bp and about 350 bp, respectively).
On the other hand, no band was observed in Lanes 2, 4, 5 and 7.

Example 3 Human interleukin-3 DNA fragments having the sequences of SEQ ID NOs: 11 and 12 were synthesized in the same manner as in Example 1. 1 μl of each of the above DNA fragments was placed in three 0.5 ml tubes to prepare a sample.
DEPC-treated water, human interleukin-3 cDNA
A reaction solution was prepared in the same manner as in Example 1 using an aqueous clone solution (50 attomole / ml) (Clontech) or an aqueous human genomic DNA solution. PCR was performed on these reaction solutions in the same manner as in Example 1, and electrophoresis was performed to observe bands. FIG. 3 is a diagram showing the appearance of bands observed by the above electrophoresis. In the figure, lane 1 shows that the molecular weight marker was electrophoresed, and the number on the left side of each band on this lane shows the molecular weight of each band (unit is base pair (bp)). Lane 2, lane 3, and lane 4 show electrophoresed reaction solutions when using DEPC-treated water, human interleukin-3 cDNA clone aqueous solution, and human genomic DNA aqueous solution as samples, respectively. In the figure, a band is observed in lane 3, and the molecular weight of this band is the expected value (about 320 b).
p). On the other hand, no band was observed in Lanes 2 and 4.

Example 4 Human interleukin-4 DNA fragments having the sequences of SEQ ID NOs: 13 to 15 were synthesized in the same manner as in Example 1. 1 μl of each of the DNA fragments having the sequences of SEQ ID NOs: 13 and 15 was placed in three 0.5 ml tubes.
1 pc each, and as a sample, DEPC-treated water, human interleukin 4 cDNA clone aqueous solution (50 attomole / ml) (Clontech) or human genomic DN
Using the aqueous solution A, a reaction solution was prepared in the same manner as in Example 1. Reaction solutions were similarly prepared for the DNA fragments having the sequences of SEQ ID NOs: 14 and 15. PCR was performed on these reaction solutions in the same manner as in Example 1, and electrophoresis was performed to observe bands. FIG. 4 is a diagram showing the appearance of bands observed by the above electrophoresis. In the figure, lane 1 shows that the molecular weight marker was electrophoresed, and the number on the left side of each band on this lane shows the molecular weight of each band (unit is base pair (bp)). Lanes 2 to 4 and 5 to 7 show electrophoresed reaction solutions when the DNA fragments having the sequences of SEQ ID NOs: 13 and 15 and 14 and 15, respectively, were used. Lanes 2 and 5, lanes 3 and 6, and lanes 4 and 7 were electrophoresed with a reaction solution using DEPC-treated water, human interleukin 4 cDNA clone aqueous solution, and human genomic DNA aqueous solution, respectively. Show things. In the figure, bands were observed in lanes 3 and 6, respectively, and the molecular weights of these bands were in agreement with the expected values (about 360 bp and about 290 bp, respectively). On the other hand, no band was observed in Lanes 2, 4, 5 and 7.

Example 5 Human interleukin-5 DNA fragments having the sequences of SEQ ID NOs: 16 and 17 were synthesized in the same manner as in Example 1. 1 μl of each of the above DNA fragments was placed in three 0.5 ml tubes to prepare a sample.
DEPC-treated water, human interleukin-5 cDNA
A reaction solution was prepared in the same manner as in Example 1 using an aqueous clone solution (50 attomole / ml) (Clontech) or an aqueous human genomic DNA solution. PCR was performed on these reaction solutions in the same manner as in Example 1, and electrophoresis was performed to observe bands. C of human interleukin 5 as a sample
A band was observed in the reaction solution using the DNA clone aqueous solution, and the molecular weight of this band was the expected value (about 280 b).
p). On the other hand, no band was observed at all when the reaction solution using DEPC-treated water and the aqueous solution of human genomic DNA were used.

Example 6 Human interleukin-6 DNA fragments having the sequences of SEQ ID NOs: 18 to 21 were synthesized in the same manner as in Example 1. 1 μl of each of the DNA fragments having the sequences of SEQ ID NOs: 18 and 21 was placed in three 0.5 ml tubes.
Each 1 μl was added, and DEPC-treated water, human interleukin 6 cDNA clone aqueous solution (50 attomole / ml) (Clontech), human monocyte cDNA library aqueous solution or human genomic DNA aqueous solution was used as a sample. A reaction solution was prepared in the same manner as in 1. Sequence number 1
Reaction solutions were similarly prepared for the DNA fragments having the sequences 9 and 20. PCR was performed on these reaction solutions in the same manner as in Example 1, and electrophoresis was performed to observe bands. Figure 5
FIG. 4 is a diagram showing the appearance of bands observed by the above-mentioned electrophoresis. In the figure, lane 1 shows that the molecular weight marker was electrophoresed, and the numbers on the left side of each band on this lane are the molecular weight of each band (unit is base pair (bp)).
Indicates. Lanes 2 to 5 and 6 to 9 show electrophoresed reaction solutions when the DNA fragments having the sequences of SEQ ID NOs: 19 and 20 and 18 and 21, respectively, were used. Lanes 2 and 6, lanes 3 and 7, lanes 4 and 8, and lanes 5 and 9, respectively, were treated with DEPC-treated water, human interleukin 6 cDNA clone aqueous solution, human monocyte cDNA library aqueous solution, and The figure shows an electrophoresed reaction solution in the case of using an aqueous solution of human genomic DNA. In the figure, bands were observed at almost the same positions in lanes 3 and 4 and lanes 7 and 8, respectively, and the molecular weights of these bands were in agreement with expected values (about 190 bp and about 430 bp, respectively). On the other hand, no band was observed in Lanes 2, 5, 6, and 9.

Example 7 Human interleukin-7 DNA fragments having the sequences of SEQ ID NOs: 22 to 24 were synthesized in the same manner as in Example 1. 1 μl of each of the DNA fragments having the sequences of SEQ ID NOs: 22 and 24 was placed in three 0.5 ml tubes.
1 liter each, as a sample, DEPC-treated water, human interleukin 7 cDNA clone aqueous solution (50 attomole / ml) (Clontech) or human genomic DN
Using the aqueous solution A, a reaction solution was prepared in the same manner as in Example 1. Reaction solutions were similarly prepared for the DNA fragments having the sequences of SEQ ID NOs: 23 and 24. PCR was performed on these reaction solutions in the same manner as in Example 1, and electrophoresis was performed to observe bands. FIG. 6 is a diagram showing the appearance of bands observed by the above electrophoresis. In the figure, lane 1 shows that the molecular weight marker was electrophoresed, and the number on the left side of each band on this lane shows the molecular weight of each band (unit is base pair (bp)). Lanes 2 to 4 and 5 to 7 show electrophoresed reaction solutions when the DNA fragments having the sequences of SEQ ID NOs: 22 and 24 and 23 and 24 were used, respectively. Lanes 2 and 5, lanes 3 and 6, and lanes 4 and 7 were electrophoresed with the reaction solutions using DEPC-treated water, human interleukin 7 cDNA clone aqueous solution, and human genomic DNA aqueous solution, respectively. It shows what was done. In the figure, bands were observed in lanes 3 and 6, respectively, and the molecular weights of these bands were expected values (about 420 bp and about 300 b, respectively).
p). On the other hand, no band was observed in Lanes 2, 4, 5 and 7.

Example 8 Human interleukin-8 DNA fragments having the sequences of SEQ ID NOs: 25 and 26 were synthesized in the same manner as in Example 1. 1 μl of each of the above DNA fragments was placed in 4 0.5 ml tubes to prepare samples.
DEPC-treated water, human interleukin-8 cDNA
Using a clone aqueous solution (50 attomole / ml) (Clontech), a human monocyte cDNA library aqueous solution, or a human genomic DNA aqueous solution, a reaction solution was prepared in the same manner as in Example 1. PCR was performed on these reaction solutions in the same manner as in Example 1, and electrophoresis was performed to observe bands.
FIG. 7 is a diagram showing the appearance of bands observed by the above electrophoresis. In the figure, lane 1 shows that the molecular weight marker was electrophoresed, and the numbers on the left side of each band on this lane indicate the molecular weight of each band (unit is base pair (b
p)) is shown. Lane 2, lane 3, lane 4, and lane 5 respectively use DEPC-treated water, human interleukin 8 cDNA clone aqueous solution, human monocyte cDNA library aqueous solution, and human genomic DNA aqueous solution as samples. When the reaction solution is present, it shows the result of electrophoresis. In the figure, bands were observed at almost the same positions in lanes 3 and 4, and the molecular weight of this band was in agreement with the expected value (about 230 bp). On the other hand, no band was observed in Lanes 2 and 5.

Example 9 Human interferon-γ DNA fragments having the sequences of SEQ ID NOs: 27 to 29 were synthesized in the same manner as in Example 1. 1 μl of each of the DNA fragments having the sequences of SEQ ID NOs: 27 and 29 was placed in two 0.5 ml tubes.
1 each, and DEPC-treated water or a human interferon-γ cDNA clone aqueous solution (50 attomole / ml) (Clontech) was used as a sample.
A reaction solution was prepared in the same manner as in. Reaction solutions were similarly prepared for the DNA fragments having the sequences of SEQ ID NOs: 28 and 29. PCR was performed on these reaction solutions in the same manner as in Example 1,
The band was observed by electrophoresis. FIG. 8 is a diagram showing the appearance of bands observed by the above electrophoresis. In the figure,
Lane 4 shows electrophoresis of molecular weight markers, and the numbers on the right side of each band on this lane indicate the molecular weight of each band (unit is base pair (bp)). Lane 1
And 3 and lane 2 are DNA of the sequences of SEQ ID NOs: 28 and 29, and SEQ ID NOs: 27 and 29, respectively.
The electrophoresed reaction solution when fragments were used is shown. Lane 1, and lanes 2 and 3, respectively, were treated with DEPC-treated water and c of human interferon-γ.
The reaction solution obtained by using the DNA clone aqueous solution is shown. In the figure, bands were observed in lanes 2 and 3, and the molecular weights of these bands were in agreement with expected values (about 320 bp and about 230 bp, respectively). On the other hand, no band was observed in Lane 1.

Example 10 Human, rat and mouse β
-Actin DNA fragments having the sequences of SEQ ID NOs: 30 and 31 were synthesized in the same manner as in Example 1. 1 μl of each of the above DNA fragments was put into 7 0.5 ml tubes to prepare samples.
DEPC-treated water, human β-actin cDNA fragment aqueous solution (50 attomole / ml) (Clontech), rat β-actin cDNA fragment aqueous solution (50 attomole / ml) (Clontech), mouse β- Actin cDNA fragment aqueous solution (50 attomole / ml) (Clontech), human brain cDNA library aqueous solution (1.2 × 10 ⁶ pfu / μl) (Clontech), mouse brain cDNA library aqueous solution (1 .2
× 10 ⁶ pfu / μl) (Clontech) or brain cDNA library aqueous rats (1.2 × 10 ⁶ p
fu / μl) (Clontech) and used in Example 1.
A reaction solution was prepared in the same manner as in. PCR was performed on these reaction solutions in the same manner as in Example 1, and electrophoresis was performed to observe bands. FIG. 9 is a diagram showing the appearance of bands observed by the above electrophoresis. In the figure, lane 8 shows that molecular weight markers were electrophoresed, and the numbers on the right side of each band on this lane show the molecular weight of each band (unit is base pair (bp)). Lane 1, Lane 2, Lane 3,
Lane 4, lane 5, lane 6, and lane 7 are samples of DEPC-treated water, human β-actin cDNA fragment aqueous solution, and rat β-actin cDNA, respectively.
A fragment aqueous solution, mouse β-actin cDNA fragment aqueous solution, human brain cDNA library aqueous solution, mouse brain cDNA library aqueous solution, and rat brain cDNA
The reaction solution obtained by using the A library aqueous solution is shown. In the figure, bands were observed at almost the same positions in lanes 2 to 7, and the molecular weights of these bands were in agreement with expected values (about 340 bp). On the other hand, no band was observed in Lane 1.

Example 11 Rat Serotonin 1c Receptor and Rat Serotonin 2 Receptor DNA fragments having the sequences of SEQ ID NOs: 32 to 35 were synthesized in the same manner as in Example 1. The method of Julius et al. ("Science, 241, 558-564, 198.
8 (Science, Vol. 241, pp.558-564 (1988)) ”, first, cloned cells of rat serotonin 1c receptor and serotonin 2 receptor were prepared. Next, Kabbler and Hoffman's method ("Gene, 25, 2
63 pages, 1983 (Gene, Vol.25, pp.263 (198
3)) ”), the rat serotonin 1c receptor and serotonin 2 receptor cdD from the cloned cells
NA was prepared. 1 μl of each of the DNA fragments having the sequences of SEQ ID NOs: 32 and 33 was placed in two 0.5 ml tubes, and DEPC-treated water or an aqueous solution of rat serotonin 1c receptor cDNA (50 attomole / ml) was used as a sample. A reaction solution was prepared in the same manner as in Example 1. On the other hand, prepare two 0.5 ml tubes separately and add 1 μ each of the DNA fragments of the sequences of SEQ ID NOs: 34 and 35.
A reaction solution was prepared in the same manner as in Example 1, using DEPC-treated water or rat serotonin 2 receptor cDNA aqueous solution (50 attomole / ml) as a sample. PCR was performed on these reaction solutions in the same manner as in Example 1, and electrophoresis was performed to observe bands.

FIG. 10 is a diagram showing the appearance of bands observed by the above electrophoresis. In the figure, lane 5 shows the result of migration of molecular weight markers, and the numbers on the right side of each band on this lane show the molecular weight of each band (unit is base pair (bp)). Lanes 1 and 3, and lanes 2 and 4 show electrophoresed reaction solutions when the DNA fragments of the sequences of SEQ ID NOs: 32 and 33 and SEQ ID NOS: 34 and 35 were used, respectively. Lanes 1 and 2, lane 3, and lane 4 were electrophoresed with a reaction solution using DEPC-treated water as a sample, a rat serotonin 1c receptor cDNA aqueous solution, and a rat serotonin 2 receptor cDNA aqueous solution, respectively. It shows what was done. In the figure, a band is observed in lane 3, and the molecular weight of this band is the expected value (about 380b).
p). On the other hand, no band was observed in Lane 1. In the figure, a band was also observed in lane 4, and the molecular weight of this band was the expected value (about 420b).
p). On the other hand, no band was observed in Lane 2.

[0046]

EFFECT OF THE INVENTION With the primer of claim 1, a specific m
RNA can be reliably detected and measured. With the primer of claim 2, human interleukin-1α, human interleukin-1β, human interleukin-2, human interleukin-3, human interleukin-4, human interleukin-5, human interleukin-6, human interleukin-6,
Human interleukin-7, human interleukin-8
Alternatively, human interferon-γ-specific mRNA can be reliably detected and measured. With the primer of claim 3, human, rat, or mouse β-actin-specific mRNA can be reliably detected and measured. With the primer of claim 4, mRNA specific to rat serotonin 1c receptor or rat serotonin 2 receptor can be reliably detected and measured.

[Sequence list]

 SEQ ID NO: 1 Sequence Length: 23 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence AGTTCCTCCA TTGATCATCT GTC 23

SEQ ID NO: 2 Sequence Length: 23 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence CCTCTAAAAC ATCCAAGCTT ACC 23

SEQ ID NO: 3 Sequence length: 25 Sequence type: Nucleic acid Number of strands: Single-stranded Sequence type: Other nucleic acid Synthetic DNA Sequence GCTCGAATTA TACTTTGATT GAGGG 25

SEQ ID NO: 4 Sequence length: 20 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence CATCCAGCTA CGAATCTCCG 20

SEQ ID NO: 5 Sequence length: 21 Sequence type: Nucleic acid Number of strands: Single-stranded Sequence type: Other nucleic acid Synthetic DNA Sequence GCACCTGTAC GATCACTGAA C 21

SEQ ID NO: 6 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single-stranded Sequence type: Other nucleic acid Synthetic DNA Sequence GGGCTTATCA TCTTTCAACA CG
22

SEQ ID NO: 7 Sequence length: 25 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence GCATTTACTG CTGGATTTAC AGATG 25

SEQ ID NO: 8 Sequence length: 25 Sequence type: Nucleic acid Number of strands: Single-stranded Sequence type: Other nucleic acid Synthetic DNA Sequence GCTCACATTT AAGTTTTACA TGCCC 25

SEQ ID NO: 9 Sequence length: 24 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence GTTGTTTCAG ATCCCTTTAG TTCC 24

SEQ ID NO: 10 Sequence length: 23 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence CAGTGTTGAG ATGATGCTTT GAC 23

SEQ ID NO: 11 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Type of sequence: Other nucleic acid Synthetic DNA Sequence CAAGCTGGGT TAACTGCTCT AA 22

SEQ ID NO: 12 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Type of sequence: Other nucleic acid Synthetic DNA Sequence GGGTTTTCAG ATAGAACGTC AG 22

SEQ ID NO: 13 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence AAGTGCGATA TCACCTTACA GG 22

SEQ ID NO: 14 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence CGAGTTGACC GTAACAGACA TC 22

SEQ ID NO: 15 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence CTCTCTCATG ATCGTCTTTA GC 22

SEQ ID NO: 16 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence GAAATTCCCA CAAGTGCATT GG 22

SEQ ID NO: 17 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence CTAGGAATTG GTTTACTCTC CG 22

SEQ ID NO: 18 Sequence length: 23 Sequence type: Nucleic acid Number of strands: Single strand Type of sequence: Other nucleic acid Synthetic DNA Sequence CAAACAAATT CGGTACATCC TCG 23

SEQ ID NO: 19 Sequence length: 24 Sequence type: Nucleic acid Number of strands: Single-stranded Sequence type: Other nucleic acid Synthetic DNA Sequence CAAGAGTAAC ATGTGTGAAA GCAG 24

SEQ ID NO: 20 Sequence length: 23 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence CCTCACTACT CTCAAATCTG TTC 23

SEQ ID NO: 21 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence GCGCAGAATG AGATGAGTTG TC 22

SEQ ID NO: 22 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence GAGTGTTCTA ATGGTCAGCA TC 22

SEQ ID NO: 23 Sequence length: 23 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence GGTATGTTTT TATTCCGTGC TGC 23

SEQ ID NO: 24 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence TCAGTGTTCT TTAGTGCCCA TC 2
Two

SEQ ID NO: 25 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence TGTGTGAAGG TGCAGTTTTG CC 22

SEQ ID NO: 26 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single-stranded Sequence type: Other nucleic acid Synthetic DNA Sequence CCCTCTTCAA AAACTTCTCC AC 22

SEQ ID NO: 27 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence TTTCAGCTCT GCATCGTTTT GG 22

SEQ ID NO: 28 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence GGTCATTCAG ATGTAGCGGA TA 22

SEQ ID NO: 29 Sequence length: 23 Sequence type: Nucleic acid Number of strands: Single stranded Sequence type: Other nucleic acid Synthetic DNA Sequence GCTTTTCGAA GTCATCTCGT TTC 23

SEQ ID NO: 30 Sequence length: 18 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence CTTCGCGGGC GACGATGC 18

SEQ ID NO: 31 Sequence length: 20 Sequence type: Nucleic acid Number of strands: Single stranded Sequence type: Other nucleic acid Synthetic DNA Sequence CGTACATGGC TGGGGTGTTG 20

SEQ ID NO: 32 Sequence length: 20 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence GTGGGACTAC TTGTCATGCC 20

SEQ ID NO: 33 Sequence length: 20 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence CGGGATGAAG AATGCCACGA 20

SEQ ID NO: 34 Sequence Length: 17 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence GGACCGCTAT GTCGCCA 17

SEQ ID NO: 35 Sequence length: 17 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence GCCTGCGTAG GAGCCTG 17

SEQ ID NO: 36 Sequence length: 2430 Sequence type: Nucleic acid Sequence type: cDNA to mRNA sequence CATTTCATTG GCGTTTGAGT CAGCAAAGAA GTCAAGATGG CCAAAGTTCC AGACATGTTT 60 GAAGACCTGA AGAACTGTTA CAGTGAAAAT GAAGAAGACA GTTCCTCCAT TGATTGATC GATCAATC ACCTCTAAAA CATCCAAGCT TACCTTCAAG 240 GAGAGCATGG TGGTAGTAGC AACCAACGGG AAGGTTCTGA AGAAGAGACG GTTGAGTTTA 300 AGCCAATCCA TCACTGATGA TGACCTGGAG GCCATCGCCA ATGACTCAGA GGAAGAAATC 360 ATCAAGCCTA GGTCAGCACC TTTTAGCTTC CTGAGCAATG TGAAATACAA CTTTATGAGG 420 ATCATCAAAT ACGAATTCAT CCTGAATGAC GCCCTCAATC AAAGTATAAT TCGAGCCAAT 480 GATCAGTACC TCACGGCTGC TGCATTACAT AATCTGGATG AAGCAGTGAA ATTTGACATG 540 GGTGCTTATA AGTCATCAAA GGATGATGCT AAAATTACCG TGATTCTAAG AATCTCAAAA 600 ACTCAATTGT ATGTGACTGC CCAAGATGAA GACCAACCAG TGCTGCTGAA GGAGATGCCT 660 GAGATACCCA AAACCATCAC AGGTAGTGAG ACCAACCTCC TCTTCTTCTG GGAAACTCAC 720 GGCACTAAGA ACTATTTCAC ATCAGTTGCC CATCCAAAC T TGTTTATTGC CACAAAGCAA 780 GACTACTGGG TGTGCTTGGC AGGGGGGCCA CCCTCTATCA CTGACTTTCA GATACTGGAA 840 AACCAGGCGT AGGTCTGGAG TCTCACTTGT CTCACTTGTG CAGTGTTGAC AGTTCATATG 900 TACCATGTAC ATGAAGAAGC TAAATCCTTT ACTGTTAGTC ATTTGCTGAG CATGTACTGA 960 CCCTTGTAAT TCTAAATGAA TGTTTACACT CTTTGTAAGA GTGGAACCAA CACTAACATA 1020 TAATGTTGTT ATTTAAAGAA CACCCTATAT TTTGCATAGT ACCAATCATT TTAATTATTA 1080 TTCTTCATAA CAATTTTAGG AGGACCAGAG CTACTGACTA TGGCTACCAA AAAGACTCTA 1140 CCCATATTAC AGATGGGCAA ATTAAGGCAT AAGAAAACTA AGAAATATGC ACAATAGCAG 1200 TTGAAACAAG AAGCCACAGA CCTAGGATTT CATGATTTCA TTTCAACTGT TTGCCTTCTA 1260 CTTTTAAGTT GCTGATGAAC TCTTAATCAA ATAGCATAAG TTTCTGGGAC CTCAGTTTTA 1320 TCATTTTCAA AATGGAGGGA ATAATACCTA AGCCTTCCTG CCGCAACAGT TTTTTATGCT 1380 AATCAGGGAG GTCATTTTGG TAAAATACTT CTTGAAGCCG AGCCTCAAGA TGAAGGCAAA 1440 GCACGAAATG TTATTTTTTA ATTATTATTT ATATATGTAT TTATAAATAT ATTTAAGATA 1500 ATTATAATAT ACTATATTTA TGGGAACCCC TTCATCCTCT GAGTGTGACC AGGCATCCTC 1560 CACAATAGCA GACAGTGTTT TCTGGGATAA GTAAGTTTGA TTTCATTA AT ACAGGGCATT 1620 TTGGTCCAAG TTGTGCTTAT CCCATAGCCA GGAAACTCTG CATTCTAGTA CTTGGGAGAC 1680 CTGTAATCAT ATAATAAATG TACATTAATT ACCTTGAGCC AGTAATTGGT CCGATCTTTG 1740 ACTCTTTTGC CATTAAACTT ACCTGGGCAT TCTTGTTTCA TTCAATTCCA CCTGCAATCA 1800 AGTCCTACAA GCTAAAATTA GATGAACTCA ACTTTGACAA CCATGAGACC ACTGTTATCA 1860 AAGTTGAGTT CATCTAATTT TAGCTTGTAG AGACGGGATT TCACCATCTT GGCCGTGCTG 1920 GTCTCGAACT TCTGACCTCG TGATCCACCC GCCTCGGCCT CCCAAAGTGC TGGGATTACA 1980 GGCGTGAGCC ATCGCGCCCG GCCTGGAGTT TCTACTGTGC ACCAGGCACT ACCTTTACAT 2040 GTATTGTTTT ATTTAATCCT CAGTCAGCCG TGTTTGGTAG GTGCAGTTAG TATATTTCCA 2100 TTTTCATCTG CGCAAACAGA TTCAGGAACT TTGTAATTTA CATAAGGTCA CATTCATCCT 2160 AATTCACAAA ATCAAGATTT CACACCTATT CCTTTTTCTT TCCAGTGCCT GTGCTTTTTC 2220 TCTCATACCA AGGAGAAGTA ATAAGCCTAA CGTTTTAAAC CTCACAAAAG TACATACAGA 2280 AAAGTAAATA GCCTAATTTT GCAACTAATA CAAATGGCGC TGTACTTCTT TGGTGATGGT 2340 AGATTTATAA TTTTTGAAGT ATGGTAGATT CAAATGAACC ACTGAAAAGG CATTTAGTTT 2400 CTTGTCCCAA ATAAAAAAAA AAAAAAAAAA 2430

SEQ ID NO: 37 Sequence length: 7824 Sequence type: Nucleic acid Sequence type: Genomic DNA sequence AAAGTATGTG CATGTATAAA TCTGTGTGTC TTCCACTTTG TCCCACATAT ACTAAATTTA 60 AACATTCTTC TAACGTAGGAATCTCAGAGTCAGTCACAGTA TTCTAAG TTATTAGTCC CCTCCCCTAA 240 GAAGCTTCCA CCAATACTCT TTTCCCCTTT CCTTTAACTT GATTGTGAAA TCAGGTATTC 300 AACAGAGAAA TTTCTCAGCC TCCTACTTCT GCTTTTGAAA GCCATAAAAA CAGCGAGGGA 360 GAAACTGGCA GATACCAAAC CTCTTCGAGG CACAAGGCAC AACAGGCTGC TCTGGGATTC 420 TCTTCAGCCA ATCTTCATTG CTCAAGTATG ACTTTAATCT TCCTTACAAC TAGGTGCTAA 480 GGGAGTCTCT CTGTCTCTCT GCCTCTTTGT GTGTATGCAT ATTCTCTCTC TCTCTCTCTT 540 TCTTTCTCTG TCTCTCCCTC TCCTTCCCTC TCTGCCTCCC TCTCTCAGCT TTTTGCAAAA 600 AGCCAGGTGT AATATAATGC TTATGACTCG GGAAATATTC TGGGAATGGA TACTGCTTAT 660 CTAACAGCTG ACACCCTAAA GGTTAGTGTC AAAGCCTCTG CTCCAGCTCT CCTAGCCTAA 720 TACATTGCTA GTTGGGGTTT GGTTTAGCAA ATGCTTTTCT CTAGACCCAA AGGACTTCTC 780 TTTCACACAT TCATTCATTT ACTCAGAGAT CATTTCTTTG CATGACTGCC ATGCACTGGA 840 TGCTGAGAGA AATCACACAT GAACGTAGCC GTCATGGGGA AGTCACTCAT TTTCTCCTTT 900 TTACACAGGT GTCTGAAGCA GCCATGGCAG AAGTACCTGA GCTCGCCAGT GAAATGATGG 960 CTTATTACAG GTCAGTGGAG ACGCTGAGAC CAGTAACATG AGCAGGTCTC CTCTTTCAAG 1020 AGTAGAGTGT TATCTGTGCT TGGAGACCAG ATTTTTCCCC TAAATTGCCT CTTTCAGTGG 1080 CAAACAGGGT GCCAAGTAAA TCTGATTTAA AGACTACTTT CCCATTACAA GTCCCTCCAG 1140 CCTTGGGACC TGGAGGCTAT CCAGATGTGT TGTTGCAAGG GCTTCCTGCA GAGGCAAATG 1200 GGGAGAAAAG ACTCCAAGCC CACAATACAA GGAATCCCTT TGCAAAGTGT GGCTTGGAGG 1260 GAGAGGGAGA GCTCAGATTT TAGCTGACTC TGCTGGGCTA GAGGTTAGGC CTCAAGATCC 1320 AACAGGGAGC NCCCAGGGTG CCCACCTGCC AGGCCTAGAA TCTGCCTTCT GGACTGTTCT 1380 GCGCATATCA CTGTGAAACT TGCCAGGTGT TTCAGGCAGC TTTGAGAGGC AGGCTGTTTG 1440 CAGTTTCTTA TGAACAGTCA AGTCTTGTAC ACAGGGAAGG AAAAATAAAC CTGTTTAGAA 1500 GACATAATTG AGACATGTCC CTGTTTTTAT TACAGTGGCA ATGAGGATGA CTTGTTCTTT 1560 GAAGCTGATG GCCCTAAACA GATGAAGGTA AGACTATGGG TTTAACTCC C AACCCAAGGA 1620 AGGGCTCTAA CACAGGGAAA GCTCAAAGAA GGGAGTTCTG GGCCACTTTG ATGCCATGGT 1680 ATTTTGTTTT AGAAAGACTT TAACCTCTTC CAGTGAGACA CAGGCTGCAC CACTTGCTGA 1740 CCTGGCCACT TGGTCATCAT ATCACCACAG TCACTCACTA ACGTTGGTGG TGGTGGCCAC 1800 ACTTGGTGGT GACAGGGGAG GAGTAGTGAT AATGTTTCCC ATTTCATAGT AGGAAGACAA 1860 CCAAGTCTTC AACATAAATT TGATTATCCT TTTAAGAGAT GGATTCAGCC TATGCCAATC 1920 ACTTGAGTTA AACTCTGAAA CCAAGAGATG ATCTTGAGAA CTAACATATG TCTACCCCTT 1980 TTGAGTAGAA TAGTTTTTTG CTACCTGGGG TGAAGCTTAT AACAACAAGA CATAGATGAT 2040 ATAAACAAAA AGATCAATTG AGACTTGAAA GAAAACCATT CACTTGCTGT TTGACCTTGA 2100 CAAGTCATTT TACCCGCTTT GGACCTCATC TGAAAAATAA AGGGCTGAGC TGGATGATCT 2160 CTGAGATTCC AGCATCCTGC AACCTCCAGT TCTGAAATAT TTTCAGTTGT AGCTAAGGGC 2220 ATTTGGGCAG CAAATGGTCA TTTTTCAGAC TCATCCTTAC AAAGAGCCAT GTTATATTCC 2280 TGCTGTCCCT TCTGTTTTAT ATGATGGCTC AGTAGCCTTC CTAGTGGCCC AGCCATCAGC 2340 CTAGCTAGGT CAGTTGTGCA GGTTGGAGGC AGCCACTTTT CTCTGGCTTT ATTTTATTCC 2400 AGTTTGTGAT AGCCTCCCCT AGCCTCATAA TCCAGTCCTC AATCTTGTTA AAAA CATATT 2460 TCTTTAGAAG TTTTAAGACT GGCATAACTT GTTGGCTGCA GCTGTGGGAG GAGCCCATTG 2520 GCTTGTCTGC CTGGCCTTTG CCCCCATTGC CTCTTCCAGC AGCTTGGCGC TGCTCCAGGC 2580 AGGAAATTCT CTCCTGCTCA ACTTTCTTTT GTGCACTTAC AGGTCTCTTT AACTGTCTTT 2640 CAAGCCTTTG AACCATTATC ACTGCCTTAA GGCAACCTCA GTGAAGCCTT AATACGGAGC 2700 TTCTCTGAAT AAGAGGAAAG TGGTAACATT TCACAAAAAG TACTCTCACA GGATTTGCAG 2760 AATGCCTATG AGACAGTGTT ATGAAAAAGG AAAAAAAAGA ACAGTGTAGA AAAATTGAAT 2820 ACTTGCTGAG TGAGCATAGG TGAATGGAAA ATGTTATGGT CATCTGCATG AAAAAGCAAA 2880 TCATAGTGTG ACAGCATTAG GGATACAAAA AGATATAGAG AAGGTATACA TGTATGGTGT 2940 AGGTGGGGCA TGTACAAAAA AGATGAACAA AGTAGAAATG GGATTTATTC TAAAGAATAG 3000 CCTGTAAGGT GTCAGAAAGC CCACATTCTA GTCTTGAGTC TGTTTCTAAC CTGCTGTGTG 3060 CCCTTGAGTA CACACTTAAC CTCTTGAGCT TCAGAGAGGG ATAATCTTTT TATTTTATTT 3120 TATTTTATTT TGTTTTGTTT TGTTTTGTTT TGTTTTATGA GACAGAGTCT CACTCTGTTG 3180 CCCAGGCTGG AGTGCAGTGG TACAATCTTG GCTTACTGCA TCCTCCACCT CCTGAGTTCA 3240 AGCGATTCTC CTTCCTCAGT CTCCTGAATA GCTAGGATTA CAGGTGCACC CCACCACACC 3300 CAGCTAATTT TTGTATTTTT AGTAGAGAAG GGGTTTCGCC ATGTTGGCCA GGCTGGTTTT 3360 GAAGTCCTGA CCTAAATGAT TCATCCACCT CGGCTTCCCA AAGTGCTGGG ATTACAGGCA 3420 TGAGCCACCA CGCCTGGCCC AGAGAGGGAT GATCTTTAGA AGCTCGGGAT TCTTTCAAGC 3480 CCTTTCCTCC TCTCTGAGCT TTCTACTCTC TGATGTCAAA GCATGGTTCC TGGCAGGACC 3540 ACCTCACCAG GCTCCCTCCC TCGCTCTCTC CGCAGTGCTC CTTCCAGGAC CTGGACCTCT 3600 GCCCTCTGGA TGGCGGCATC CAGCTACGAA TCTCCGACCA CCACTACAGC AAGGGCTTCA 3660 GGCAGGCCGC GTCAGTTGTT GTGGCCATGG ACAAGCTGAG GAAGATGCTG GTTCCCTGCC 3720 CACAGACCTT CCAGGAGAAT GACCTGAGCA CCTTCTTTCC CTTCATCTTT GAAGAAGGTA 3780 GTTAGCCAAG AGCAGGCAGT AGATCTCCAC TTGTGTCCTC TTGGAAGTCA TCAAGCCCCA 3840 GCCAACTCAA TTCCCCCAGA GCCAAAGCCC TTTAAAGGTA GAAGGCCCAG CGGGGAGACA 3900 AAACAAAGAA GGCTGGAAAC CAAAGCAATC ATCTCTTTAG TGGAAACTAT TCTTAAAGAA 3960 GATCTTGATG GCTACTGACA TTTGCAACTC CCTCACTCTT TCTCAGGGGC CTTTCACTTA 4020 CATTGTCACC AGAGGTTCGT AACCTCCCTG TGGGCTAGTG TTATGACCAT CACCATTTTA 4080 CCTAAGTAGC TCTGTTGCTC GGCCACAGTG AGCAGTAATA GACCTGAAGC TGGAACCCAT 4140 GTCTAATAGT GTCAGGTCCA TGTTCTTAGC CACCCCACTC CCAGCTTCAT CCCTACTGGT 4200 GTTGTCATCA GACTTTGACC GTATATGCTC AGTGTCCTCC AAGAAATCAA ATTTTGCCGC 4260 CTCGCCTCAC GAGGCCTGCC CTTCTGATTT TATACCTAAA CAACATGTGC TCCACATTTC 4320 AGAACCTATC TTCTTCGACA CATGGGATAA CGAGGCTTAT GTGCACGATG CACCTGTACG 4380 ATCACTGAAC TGCACGCTCC GGGACTCACA GCAAAAAAGC TTGGTGATGT CTGGTCCATA 4440 TGAACTGAAA GCTCTCCACC TCCAGGGACA GGATATGGAG CAACAAGGTA AATGGAAACA 4500 TCCTGGTTTC CCTGCCTGGC CTCCTGGCAG CTTGCTAATT CTCCATGTTT TAAACAAAGT 4560 AGAAAGTTAA TTTAAGGCAA ATGATCAACA CAAGTGAAAA AAAATATTAA AAAGGAATAT 4620 ACAAACTTTG GTCCTAGAAA TGGCACATTT GATTGCACTG GCCAGTGCAT TTGTTAACAG 4680 GAGTGTGACC CTGAGAAATT AGACGGTCAA GCACTCCCAG GACCATGTCC ACCCAAGTCT 4740 CTTGGGCATA GTGCAATGTC AATTCTTCCA CAATATCCCC TCATTTGATG GACATGGCCT 4800 AACTGCCTGT GGGTTCTCTC TTCCTGTTGT TGAGGCTGAA ACAAGAGTGC TGGAGCGATA 4860 ATGTGTCCAT CCCCTCCCCA GTCTTCCCCC CTTGCCCCAA CAGTCCGTCC CACCCAATGC 4920 AGGTGGTTCT TGTAGGGAAA TTTTACCGCC CAGCAGGAAC TTATATCTCT CCGCTGTAAC 4980 GGGCAA AAGT TTCAAGTGCG GTGAACCCAT CATTAGCTGT GGTGATCTGC CTGGCATCGT 5040 GCCACAGTAG CCAAAGCCTC TGCACAGGAG TGTGGGCAAC TAAGGCTGCT GACTTTGAAG 5100 GACAGCCTCA CTCAGGGGGA AGCTATTTGC TCTCAGCCAG GCCAAGAAAA TCCTGTTTCT 5160 TTGGAATCGG GTAGTAAGAG TGATCCCAGG GCCTCCAATT GACACTGCTG TGACTGAGGA 5220 AGATCAAAAT GAGTGTCTCT CTTTGGAGCC ACTTTCCCAG CTCAGCCTCT CCTCTCCCAG 5280 TTTCTTCCCA TGGGCTACTC TCTGTTCCTG AAACAGTTCT GGTGCCTGAT TTCTGGCAGA 5340 AGTACAGCTT CACCTCTTTC CTTTCCTTCC ACATTGATCA AGTTGTTCCG CTCCTGTGGA 5400 TGGGCACATT GCCAGCCAGT GACACAATGG CTTCCTTCCT TCCTTCCTTC AGCATTTAAA 5460 ATGTAGACCC TCTTTCATTC TCCGTTCCTA CTGCTATGAG GCTCTGAGAA ACCTCAGGCC 5520 TTTGAGGGGA AACCCTAAAT CAACAAAATG ACCCTGCTAT TGTCTGTGAG AAGTCAAGTT 5580 ATCCTGTGTC TTAGGCCAAG GAACCTCACT GTGGGTTCCC ACAGAGGCTA CCAAATTACA 5640 TGTATCCTAC TCATGGGGCC TAGGGGTTGG GGTGACCCTG CACTGCTGTG TCCCTAACCA 5700 CAAGACCCCC TTCTTTCTTC AGTGGTGTTC TCCATGTCCT TTGTACAAGG AGAAGAAAGT 5760 AATGACAAAA TACCTGTGGC CTTGGGCCTC AAGGAAAAGA ATCTGTACCT GTCCTGCGTG 5820 TTGAAAGATG A TAAGCCCAC TCTACAGCTG GAGGTAAGTG AATGCTATGG AATGAAGCCC 5880 TTCTCAGCCT CCTGCTACCA CTTATTCCCA GACAACCACC TTCTCCCCGC CCCCATCCCT 5940 AGGAAAAGCT GGGAACAGGT CTATTTGACA ATTTTGCATT AATGTAAATA AATTTAACAT 6000 AATTTTTAAC TGCGTGCAAC CTTCAATCCT GCTGCAGAAA ATTAAATCAT TTTGCCGATG 6060 TTATTATGTC CTACCATAGT TACAACCCCA ACAGATTATA TATTGTTAGG GCTGCTCTCA 6120 TTTGATAGAC ACCTTGGGAA ATAGATGACT TAAAGGGTCC CATTATCACG TCCACTCCAC 6180 TCCCAAAATC ACCACCACTA TCACCTCCAG CTTTCTCAGC AAAAGCTTCA TTTCCAAGTT 6240 GATGTCATTC TAGGACCATA AGGAAAAATA CAATAAAAAG CCCCTGGAAA CTAGGTACTT 6300 CAAGAAGCTC TAGCTTAATT TTCACCCCCC AAAAAAAAAA AATTCTCACC TACATTATGC 6360 TCCTCAGCAT TTGGCACTAA GTTTTAGAAA AGAAGAGGGC TCTTTTAAAT AAATTCACAC 6420 AGAAAGTTGG GCCCAGTTAC AACTCAGGAG TCTGGCTCCT GATCATGTGA CCTGCTCGTC 6480 AGTTTCCTTT CTGGCCAACC CAAAGAACAT CTTTCCCATA GCATCTTTGT CCCTTGCCCC 6540 ACAAAAATTC TTCTTTCTCT TTCGTGCAGA GTGTAGATCC CAAAAATTAC CCAAAGAAGA 6600 AGATGGAAAA GCGATTTGTC TTCAACAAGA TAGAAATCAA TAACAAGCTG GAATTTGAGT 6660 CTGCCCAGTT CCCCAAC TGG TACATCAGCA CCTCTCAAGC AGAAAACATG CCCGTCTTCC 6720 TGGGAGGGAC CAAAGGCGGC CAGGATATAA CTGACTTCAC CATGCAATTT GTGTCTTCCT 6780 AAAGAGAGCT GTACCCAGAG AGTCCTGTGC TGAATGTGGA CTCAATCCCT AGGGCTGGCA 6840 GAAAGGGAAC AGAAAGGTTT TTGAGTACGG CTATAGCCTG GACTTTCCTG TTGTCTACAC 6900 CAATGCCCAA CTGCCTGCCT TAGGGTAGTG CTAAGAGGAT CTCCTGTCCA TCAGCCAGGA 6960 CAGTCAGCTC TCTCCTTTCA GGGCCAATCC CCAGCCCTTT TGTTGAGCCA GGCCTCTCTC 7020 ACCTCTCCTA CTCACTTAAA GCCCGCCTGA CAGAAACCAC GGCCACATTT GGTTCTAAGA 7080 AACCCTCTGT CATTCGCTCC CACATTCTGA TGAGCAACCG CTTCCCTATT TATTTATTTA 7140 TTTGTTTGTT TGTTTTATTC ATTGGTCTAA TTTATTCAAA GGGGGCAAGA AGTAGCAGTG 7200 TCTGTAAAAG AGCCTAGTTT TTAATAGCTA TGGAATCAAT TCAATTTGGA CTGGTGTGCT 7260 CTCTTTAAAT CAAGTCCTTT AATTAAGACT GAAAATATAT AAGCTCAGAT TATTTAAATG 7320 GGAATATTTA TAAATGAGCA AATATCATAC TGTTCAATGG TTCTGAAATA AACTTCACTG 7380 AAGAAAAAAA AAGGGTCTTT CCTGATCATT GACTTGTCTT GGATTTGACA CTGAACAGTA 7440 AAGACAAACA GGGCTGTGAG AGTTCTTGGG GGACTAAAGC CCACCTCCTC ATTGCTGAGT 7500 GCTGCAAAGT CACCTAGAAA TA TCCCTTGG CCACCGAAGA CTATCCTCCT CACCCATCCC 7560 CTTTATTTCT GTTGTTCAAC AGAAGGATAT TCAGTGCACA TCTGGAACAG GATCAGCTGA 7620 AGCACTGCAG GGAGTCAGGA CTGGTAGTAA CAGCTACCAG TGATTTATCT ATCAATGCAC 7680 CAAACATCTG TTGAGCAAGC GCTATGTACG AGGAGCTGGG AGTACAGAGA TGAGAACAGT 7740 CACAAGTCCC TCCTCAGATA GGAGAGGCAG CTAGTTATAA GCAGAAACAA GGTAACATGA 7800 CAAGTAGAGT AAGATAAAGA ACAA 7824

[0083] SEQ ID NO: 38 SEQ Length: 5737 Type of sequence: nucleic acid sequence of the type: Genomic DNA sequence TTAATCAACA AATCTAAACA TTTATTCTTT TCATCTGTTT ACTCTTGCTC TTGTTCACCA 60 CAATATGCTA TTCACATGTT CAGTGTAGTT TTATGACAAA GAAAATTTTC TGAGTTACTT 120 TTGTATCCCC ACCCCCTTAA AGAAAGGAGG AAAAACTGTT TCATACAGAA GGCGTTAATT 180 GCATGAATTA GAGCTATCAC CTAAGTGTGG GCTAATGTAA CAAAGAGGGA TTTCACCTAC 240 ATCCATTCAG TCAGTCTTTG GGGGTTTAAA GAATTCCAAA GAGTCATCAG AAGAGGAAAA 300 ATGAAGGTAA TGTTTTTTCA GACAGGTAAA GTCTTTGAAA ATATGTGTAA TATGTAAAAC 360 ATTTTGACAC CCCCATAATA TTTTTCCAGA ATTAACAGTA TAAATTGCAT CTCTTGTTCA 420 AGAGTTCCCT ATCACTCTCT TTAATCACTA CTCACAGTAA CCTCAACTCC TGCCACAATG 480 TACAGGATGC AACTCCTGTC TTGCATTGCA CTAAGTCTTG CACTTGTCAC AAACAGTGCA 540 CCTACTTCAA GTTCTACAAA GAAAACACAG CTACAACTGG AGCATTTACT GCTGGATTTA 600 CAGATGATTT TGAATGGAAT TAATGTAAGT ATATTTCCTT TCTTACTAAA ATTATTACAT 660 TTAGTAATCT AGCTGGAGAT CATTTCTTAA TAACAATGCA TTATACTTTC TTAGAATTAC 720 AAGAATCCCA AACTCACCAG GATGCTCACA TTTAAGTTTT ACATGCCCAA GAAGGTAAGT 780 ACAATATTTT ATGTTCAATT TCTGTTTTAA TAAAATTCAA AGTAATATGA AAATTTGCAC 840 AGATGGGACT AATAGCAGCT CATCTGAGGT AAAGAGTAAC TTTAATTTGT TTTTTTGAAA 900 ACCCAAGTTT GATAATGAAG CCTCTATTAA AACAGTTTTA CCTATATTTT TAATATATAT 960 TTGTGTGTTG GTGGGGGTGG GAAGAAAACA TAAAAATAAT ATTCTCACCT TTATCGATAA 1020 GACAATTCTA AACAAAAATG TTCATTTATG GTTTCATTTA AAAATGTAAA ACTCTAAAAT 1080 ATTTGATTAT GTCATTTTAG TATGTAAAAT ACCAAAATCT ATTTCCAAGG AGCCCACTTT 1140 TAAAAATCTT TTCTTGTTTT AGGAAAGGTT TCTAAGTGAG AGGCAGCATA ACACTAATAG 1200 CACAGAGTCT GGGGCCAGAT ATCTGAAGTG AAATCTCAGC TCTGCCATGT CCTAGCTTTC 1260 ATGATCTTTG GCAAATTACC TACTCTGTTT GTGATTCAGT TTCATGTCTA CTTAAATGAA 1320 TAACTGTATA TACTTAATAT GGCTTTGTGA GAATTAGTAA GTTAAATGTA AAGCACTCAG 1380 AACCGTGTCT GGCATAAGGT AAATACCATA CAAGCATTAG CTATTATTAG TAGTATTAAA 1440 GATAAAATTT TCACTGAGAA ATACAAAGTA AAATTTTGGA CTTTATCTTT TTACCAATAG 1500 AACTTGAGAT TTATAATGCT ATATGACTTA TTTTCCAAGA TTAAAAGCTT CATTAGGTTG 1560 TTTTTGGATT CAGATAGAGC ATAAGCATAA TCATCCAAGC TCCTAGGCT A CATTAGGTGT 1620 GTAAAGCTAC CTAGTAGTTG TGCCAGTTAA GAGAGAATGA ACAAAATCTG GTGCCAGAAA 1680 GAGCTTGTGC CAGGGTGAAT CCAAGCCCAG AAAATAATAG GATTTAAGGG GACACAGATG 1740 CAATCCCATT GACTCAAATT CTATTAATTC AAGAGAAATC TGCTTCTAAC TACCCTTCTG 1800 AAAGATGTAA AGGAGACAGC TTACAGATGT TACTCTAGTT TAATCAGAGC CACATAATGC 1860 AACTCCAGCA ACATAAAGAT ACTAGATGCT GTTTTCTGAA GAAAATTTCT CCACATTGTT 1920 CATGCCAAAA ACTTAAACCC GAATTTGTAG AATTTGTAGT GGTGAATTGA AAGCGCAATA 1980 GATGGACATA TCAGGGGATT GGTATTGTCT TGACCTACCT TTCCCACTAA AGAGTGTTAG 2040 AAAGATGAGA TTATGTGCAT AATTTAGGGG GTGGTAGAAT TCATGGAAAT CTAAGTTTGA 2100 AACCAAAAGT AATGATAAAC TCTATTCATT TGTTCATTTA ACCCTCATTG CACATTTACA 2160 AAAGATTTTA GAAACTAATA AAAATATTTG ATTCCAAGGA TGCTATGTTA ATGCTATAAT 2220 GAGAAAGAAA TGAAATCTAA TTCTGGCTCT ACCTACTTAT GTGGTCAAAT TCTGAGATTT 2280 AGTGTGCTTA TTTATAAAGT GGAGATGATA CTTCACTGCC TACTTCAAAA GATGACTGTG 2340 AGAAGTAAAT GGGCCTATTT TGGAGAAAAT TCTTTTAAAT TGTAATATAC CATAGAAATA 2400 TGAAATATTA TATATAATAT AGAATCAAGA GGCCTGTCCA AAAGTCCTCC CAAA GTATTA 2460 TAATCTTTTA TTTCACTGGG ACAAACATTT TTAAAATGCA TCTTAATGTA GTGATTGTAG 2520 AAAAGTAAAA TTTAAGACAT ATTTAAAAAT GTGTCTTGCT CAAGGCTATA TTGAGAGCCA 2580 CTACTACATG ATTATTGTTA CCTAGTGTAA AATGTTGGGA TTGTGATAGA TGGCATTCAA 2640 GAGTTCCTTC TCTCTCAACA TTCTGTGATT CTTAACTCTT AGACTATCAA ATATTATAAT 2700 CATAGAATGT GATTTTTATG CTTCCACATT CTAACTCATC TGGTTCTAAT GATTTTCTAT 2760 GCAGATTGGA AAAGTAATCA GCCTGCATCT GTGATAGGCA CTTACGATGC AGAAAGTCTA 2820 ACATTTTGCA AAGCCAAATT AAGCTAAAAC CAGTGAGTCA ACTATCACTT AACGCTAGTC 2880 ATAGGTACTT GAGCCCTAGT TTTTCCAGTT TTATAATGTA AACTCTACTG GTCCATTCTT 2940 TACAGTGACA TTGAGAACAG AGAGAATGGT AAAAACTACA TACTGCTACT CCAAATAAAA 3000 TAAATTGGAA ATTAATTTCT GATTCTGACC TCTATGTAAA CTGAGCTGAT GATAATTATT 3060 ATTCTAGGCC ACAGAACTGA AACATCTTCA GTGTCTAGAA GAAGAACTCA AACCTCTGGA 3120 GGAAGTGCTA AATTTAGCTC AAAGCAAAAA CTTTCACTTA AGACCCAGGG ACTTAATCAG 3180 CAATATCAAC GTAATAGTTC TGGAACTAAA GGTAAGGCAT TACTTTATTT GCTCTCCTGG 3240 AAATAAAAAA AAAAAAGTCA GGGGGAAAAG TACCACATTT TAAAGTGACA TAACATTTTT 3300 GGTATTTGTA AAGTACCCAT GCATGTAATT AGCCTACATT TTAAGTACAC TGTGAACATG 3360 AATCATTTCT AATGTTAAAT GATTAACTGG GGAGTATAAG CTACTGAGTT TGCACCTACC 3420 ATCTACTAAT GGACAAGCCT CATCCCAAAC TCCATCACCT TTCATATTAA CACAAAACTG 3480 GGAGTGAGAG AAGGTACTGA GTTGAGTTTC ACAGAAAGCA GGCAGATTTT ATTATATATT 3540 TTTCGATTCT TCAGATCATT TACTGAAATA GCCAATACTG ATTACCTGAA AGGCTTTTCA 3600 AATGGTGTTT CCTTATCATT TGATGGAAGG ACTACCCATA AGAGATTTGT CTTAAAAAAA 3660 AAAACTGGAG CCATTAAAAT GGCCAGTGGA CTAAACAAAC AACAATCTTT TTAGAGGCAA 3720 TCCCCACTTT CAGAATCTTA AGTATTTTTA AATGCACAGG AAGCATAAAA TATGCAAGGG 3780 ACTCAGGTGA TGTAAAAGAG ATTCACTTTT GTCTTTTTAT ATCCCGTCTC CTAAGGTATA 3840 AAATTCATGA GTTAATAGGT ATCCTAAATA AGCAGCATAA GTATAGTAGT AAAAGACATT 3900 CCTAAAAGTA ACTCCAGTTG TGTCCAAATG AATCACTTAT TAGTGGACTG TTTCAGTTGA 3960 ATTAAAAAAA TACATTGAGA TCAATGTCAT CTAGACATTG ACAGATTCAG TTCCTTATCT 4020 ATGGCAAGAG TTTTACTCTA AAATAATTAA CATCAGAAAA CTCATTCTTA ACTCTTGATA 4080 CAAATTTAAG ACAAAACCAT GCAAAAATCT GAAAACTGTG TTTCAAAAGC CAAACACTTT 4140 TTAAAATAAA AAATCCCAAG ATATGACAAT ATTTAAACAA TTATGCTTAA GAGGATACAG 4200 AACACTGCAA CAGTTTTTTA AAAGAGAATA CTTATTTAAA GGGAACACTC TATCTCACCT 4260 GCTTTTGTTC CCAGGGTAGG AATCACTTCA AATTTGAAAA GCTCTCTTTT AAATCTCACT 4320 ATATATCAAA ATATTTCCTC CTTAGCTTAT CAACTAGAGG AAGCGTTTAA ATAGCTCCTT 4380 TCAGCAGAGA AGCCTAATTT CTAAAAAGCC AGTCCACAGA ACAAAATTTC TAATGTTTAA 4440 ACTTTTAAAA GTTGGCAAAT TCACCTGCAT TGATACTATG ATGGGGTAGG GATAGGTGTA 4500 AGTATTTAGA AGATGTTCTT CACACAAATT TATCCCAAAC GGAAGCATGT CCTAGCTTAC 4560 TCTAGTGTAG TTCTGTTCTG CTTTGGGGAA AATATAAGGA GATTCACTTA AGTAGAAAAA 4620 TAGGAGACTC TAATCAAGAT TTAGAAAAGA AGAAAGTATA ATGTGCATAT CAATTCATAC 4680 ATTTAACTTA CACAAATATA GGTGTACATT CAGAGGAAAA GCGATCAAGT TTATTTCACA 4740 TCCAGCATTT AATATTTGTC TAGATCTATT TTTATTTAAA TCTTTATTTG CACCCAATTT 4800 AGGGAAAAAA TTTTTGTGTT CATTGACTGA ATTAACAAAT GAGGAAAATC TCAGCTTCTG 4860 TGTTACTATC ATTTGGTATC ATAACAAAAT ATGTAATTTT GGCATTCATT TTGATCATTT 4920 CAAGAAAATG CGAATAATTA ATATGTTTGG TAAGCTTGAA AATAAAGGCA ACAGGCCTAT 4980 AAGACT TCAA TTGGGAATAA CTGTATATAA GGTAAACTAC TCTGTACTTT AAAAAATTAA 5040 CATTTTTCTT TTATAGGGAT CTGAAACAAC ATTCATGTGT GAATATGCTG ATGAGACAGC 5100 AACCATTGTA GAATTTCTGA ACAGATGGAT TACCTTTTGT CAAAGCATCA TCTCAACACT 5160 GACTTGATAA TTAAGTGCTT CCCACTTAAA ACATATCAGG CCTTCTATTT ATTTAAATAT 5220 TTAAATTTTA TATTTATTGT TGAATGTATG GTTTGCTACC TATTGTAACT ATTATTCTTA 5280 ATCTTAAAAC TATAAATATG GATCTTTTAT GATTCTTTTT GTGCCCTAGG GGCTCTAAAA 5340 TGGTTTCACT TATTTATCCC AAAATATTTA TTATTATGTT GAATGTTAAA TATAGTGCTA 5400 TGTAGATTGG TTAGTAAAAC TATTTAATAA ATTTGATAAA TATAAACAAG CCTGGATATT 5460 TGTTATTTTG GAAACAGCAC AGAGTAAGCA TTTAAATATT TCTTAGTTAC TTGTGTGAAC 5520 TGTAGGATGG TTAAAATGCT TACAAAAGTC ACTCTTTCTC TGAAGAAATA TGTAGAACAG 5580 AGATGTAGAC TTCTCAAAAG CCCTTGCTTT GTCCTTTCAA GGGCTGATCA GACCCTTAGT 5640 TCTGGCATCT CTTAGCAGAT TATATTTTCC TTCTTCTTAA AATGCCAAAC ACAAACACTC 5700 TTGAAACTCT TCATAGATTT GGTGTGGCTA TGAATTC 5737

SEQ ID NO: 39 Sequence length: 923 Sequence type: Nucleic acid Sequence type: cDNA to mRNA sequence CAGAGCCCCA CGAAGGACCA GAACAAGACA GAGTGCCTCC TGCCGATCCA AACATGAGCC 60 GCCTGCCCGTCCTGTAGCTACTC TAGGACCCTACATCTACTCTAGGCATCCTAGGTACTCGAGGACTCTAGCTACGACGACGACGACGACGACCAGGAG TGCTGGACTT CAACAACCTC AATGGGGAAG 240 ACCAAGACAT TCTGATGGAA AATAACCTTC GAAGGCCAAA CCTGGAGGCA TTCAACAGGG 300 CTGTCAAGAG TTTACAGAAC GCATCAGCAA TTGAGAGCAT TCTTAAAAAT CTCCTGCCAT 360 GTCTGCCCCT GGCCACGGCC GCACCCACGC GACATCCAAT CCATATCAAG GACGGTGACT 420 GGAATGAATT CCGGAGGAAA CTGACGTTCT ATCTGAAAAC CCTTGAGAAT GCGCAGGCTC 480 AACAGACGAC TTTGAGCCTC GCGATCTTTT AGTCCAACGT CCAGCTCGTT CTCTGGGCCT 540 TCTCACCACA GAGCCTCGGG ACATCAAAAA CAGCAGAACT TCTGAAACCT CTGGGTCATC 600 TCTCACACAT TCCAGGACCA GAAGCATTTC ACCTTTTCCT GCGGCATCAG ATGAATTGTT 660 AATTATCTAA TTTCTGAAAT GTGCAGCTCC CATTTGGCCT TGTGCGGTTG TGTTCTCATT 720 TTTATCCCAT TGAGAC TATT TATTTATGTA TGTATGTATT TATTTATTTA TTGCCTGGAG 780 TGTGAACTGT ATTTATTTTA GCAGAGGAGC CATGTCCTGC TGCTTCTGCA AAAAACTCAG 840 AGTGGGGTGG GGAGCATGTT CATTTGTACC TCGAGTTTTA AACTGGTTCC TAGGGATGTG 900 TGAGAATAAA C923

SEQ ID NO: 40 Sequence length: 614 Sequence type: Nucleic acid Sequence type: cDNA to mRNA sequence GATCGTTAGC TTCTCCTGAT AAACTAATTG CCTCACATTG TCACTGCAAA TCGACACCTA 60 TTAATGGGTC TCACCCATCAGACACTGCTCAACACTGCTTCCAACACTGTCTGTCCAC ATTTGCGTCCAGTGTCGCGC ATGTGCCGCC ATGTGCCGCC ATGTGCCGCC 120GT GAGTTGACCG TAACAGACAT CTTTGCTGCC 240 TCCAAGAACA CAACTGAGAA GGAAACCTTC TGCAGGGCTG CGACTGTGCT CCGGCAGTTC 300 TACAGCCACC ATGAGAAGGA CACTCGCTGC CTGGGTGCGA CTGCACAGCA GTTCCACAGG 360 CACAAGCAGC TGATCCGATT CCTGAAACGG CTCGACAGGA ACCTCTGGGG CCTGGCGGGC 420 TTGAATTCCT GTCCTGTGAA GGAAGCCAAC CAGAGTACGT TGGAAAACTT CTTGGAAAGG 480 CTAAAGACGA TCATGAGAGA GAAATATTCA AAGTGTTCGA GCTGAATATT TTAATTTATG 540 AGTTTTTGAT AGCTTTATTT TTTAAGTATT TATATATTTA TAACTCATCA TAAAATAAAG 600 TATATATAGA ATCT 614

SEQ ID NO: 41 Sequence length: 3230 Sequence type: Nucleic acid Sequence type: Genomic DNA sequence ATCCTAATCA AGACCCCAGT GAACAGAACT CGACCCTGCC AAGGCTTGGC ATTTCCATTT 60 CAATCACTGT CTTCATTATTATCTAGTATTAGTTAGCACATA GTATTTTCAA TTTCTTTT A GACAGATTAA TCTAGCCAC AGTTAGCACATA GTTAGTTACA TTTCTTATTA GACAGATTAA TCTAGCCACT CACACATCTT TTAAGAGGAA 240 GTTAATTTTC TGATTTTAAG AAATGCAAAT GTGGGGCAAT GATGTATTAA CCCAAAGATT 300 CCTTCCGTAA TAGAAAATGT TTTTAAAGGG GGGAAACAGG GATTTTTATT ATTAAAAGAT 360 AAAAGTAAAT TTATTTTTTA AGATATAAGG CATTGGAAAC ATTTAGTTTC ACGATATGCC 420 ATTATTAGGC ATTCTCTATC TGATTGTTAG AAATTATTCA TTTCCTCAAA GACAGACAAT 480 AAATTGACTG GGGACGCAGT CTTGTACTAT GCACTTTCTT TGCCAAAGGC AAACGCAGAA 540 CGTTTCAGAG CCATGAGGAT GCTTCTGCAT TTGAGTTTGC TAGCTCTTGG AGCTGCCTAC 600 GTGTATGCCA TCCCCACAGA AATTCCCACA AGTGCATTGG TGAAAGAGAC CTTGGCACTG 660 CTTTCTACTC ATCGAACTCT GCTGATAGCC AATGAGGTAA TTTTCTTTAT GATTCCTACA 720 GTCTGTAAAG TGCATAGGTA ATCATTTGTG ATGGTTCCTT TACTATATAT AGAGATCTGT 780 TATAAATAAT AAGATTCTGA GCACATTAGT ACATGGGTGA TAACTACATC ACCAGCAAAC 840 ATTCTGTTAA AAGTTATGAA TGCTGGTGTG CTGTAAAAAT GATTGTATTT CCTTTCCTCT 900 CCAGACTCTG AGGATTCCTG TTCCTGTACA TAAAAATGTA AGTTAAATTA TGATTCAGTA 960 AAATGATGGC ATGAATAAGT AAATTTCCTG TTTTAAGCTG TAAATCATTA GTTATCATTG 1020 GAACTATTTA ATTTTCTATA TTTTGTTTTC ATATGGGTGG CTGTGAATGT CTGTACTTAT 1080 AAATATGAGG AATGACTTTT TATCAAGTAG AATCCTTTAA ACAAGTGGAT TAGGCTCTTT 1140 GGTGATGTTG TTAGTTTGCC TTCCCAAAGA GCATCGTGTC AGGATTCTTT CCAGAAGGAT 1200 TCCACACTGA GTGAGAGGTG CGTGCTAGTC TCCGTGCAGT TCTGACTCTT TCTCACTCTA 1260 ACGTGTTTCT GAAAGTATTA GCAACTCAGA ATTATATTTT TAGAACCATG ATCAGTAGAC 1320 ATTAAAATAT ATAACAAATG CCCTATATTA ATAATTCTGC ATACTTAAAT AATTATGACT 1380 ATATGATGGT GTGTATGCAT TGAATATGCC TGGTCATATT AAAATGTAAA ATATATAGTT 1440 TATTAGTCTA AATAGAATAA AACTACCAGC TAGAACTGTA GAAACACATT GATATGAGTT 1500 TAATGTATAA TGCATTACAC TTCCAAAACA TTTTTTTCCA GTTACATAAT TAAGTTATAT 1560 CCTTTATAAA ACTCCTCAGT AATCATATAA GCTTCATCTA CTTTTTGAA A ATTTTATCTT 1620 AATATGTGGT GGTTTGTTGC CTAGAAAACA AACAAAAAAC TCTTTGGAGA AGGGAACTCA 1680 TGTAAATACC ACAAAACAAA GCCTAACTTT GTGGACCAAA ATTGTTTTAA TAATTATTTT 1740 TTAATTGATG AATTAAAAAG TATATATATT TATTGTGTAC AATATGATGT TTTGAAGTAT 1800 GTATACATTG CAGAATGGAC AATGGACCAA ATTTTTATAC CTTGTCTTGA TTATTTGCAT 1860 TTTAAAAATT TTCCTCATTT AGCACCAACT GTGCACTGAA GAAATCTTTC AGGGAATAGG 1920 CACACTGGAG AGTCAAACTG TGCAAGGGGG TACTGTGGAA AGACTATTCA AAAACTTGTC 1980 CTTAATAAAG AAATACATTG ACGGCCAAAA AGTAAGTTAC ACACATTCAA TGGAAGCTAT 2040 ATTTGTCCTG GCTGTGCCTA TTTCTATGGA ATTGACAGTT TCCTGTAATA CCTATTGTCA 2100 TTTTTCTTTT TTCACAGAAA AAGTGTGGAG AAGAAAGACG GAGAGTAAAC CAATTCCTAG 2160 ACTACCTGCA AGAGTTTCTT GGTGTAATGA ACACCGAGTG GATAATAGAA AGTTGAGACT 2220 AAACTGGTTT GTTGCAGCCA AAGATTTTGG AGGAGAAGGA CATTTTACTG CAGTGAGAAT 2280 GAGGGCCAAG AAAGAGTCAG GCCTTAATTT TCAATATAAT TTAACTTCAG AGGGAAAGTA 2340 AATATTTCAG GCATACTGAC ACTTTGCCAG AAAGCATAAA ATTCTTAAAA TATATTTCAG 2400 ATATCAGAAT CATTGAAGTA TTTTCCTCCA GGCAAAATTG ATATACTTTT TTCT TATTTA 2460 ACTTAACATT CTGTAAAATG TCTGTTAACT TAATAGTATT TATGAAATGG TTAAGAATTT 2520 GGTAAATTAG TATTTATTTA ATGTTATGTT GTGTTCTAAT AAAACAAAAA TAGACAACTG 2580 TTCAATTTGC TGCTGGCCTC TGTCCTTAGC AATTTGAAGT TAGCACAGTC CATTGAGTAC 2640 ATGCCCAGTT TGGAGGAAGG GTCTGAGCAC ATGTGGCTGA GCATCCCCAT TTCTCTGGAG 2700 AAGTCTCAAG GTTGCAAGGC ACACCAGAGG TGGAAGTGAT CTAGCAGGAC TTAGTGGGGA 2760 TGTGGGGAGC AGGGACACAG GCAGGAGGTG AACCTGGTTT TCTCTCTACA GTATATCCAG 2820 AACCTGGGAT GGTCGAAGGG TAAATGGTAG GGAATAAATG AATGAATGTC GTTTCCAAGA 2880 TGATTGTAGA ACTAAAATGA GTTGTAAGCT CCCCTGGAAG AAGGGATGTG GAACCTGTAA 2940 CTAGGTTCCT GCCCAGCCTG TGAGAAGAAT TTGGCAGATC ATCTCATTGC CAGTATAGAG 3000 AGGAAGCCAG AAACCCTCTC TGCCAAGGCC TGCAGGGGTT CTTACCACCT GACCCTGCAC 3060 CATAACAAAA GGACAGAGAG ACATGGTAGG GCAGTCCCAT TAGAAAGACT GAGTTCCGTA 3120 TTCCCGGGGC AGGGCAGCAC CAGGCCGCAC AACATCCATT CTGCCTGCTT ATGGCTATCA 3180 GTAGCATCAC TAGAGATTCT TCTGTTTGAG AAAACTTCTC TCAAGGATCC 3230

SEQ ID NO: 42 Sequence length: 1128 Sequence type: Nucleic acid Sequence type: cDNA to mRNA sequence ATTCTGCCCT CGAGCCCACC GGGAACGAAA GAGAAGCTCT ATCTCCCCTC AGGCGCCTC AGGCGCCTC AGGCGCCTC AGGCGTTCC GGTCCAGTGAACTAGCCTCCCCT CTCTCTCCCT 120 TCAGAACGAA TTGACAAACA AATTCGGTAC 240 ATCCTCGACG GCATCTCAGC CCTGAGAAAG GAGACATGTA ACAAGAGTAA CATGTGTGAA 300 AGCAGCAAAG AGGCACTGGC AGAAAACAAC CTGAACCTTC CAAAGATGGC TGAAAAAGAT 360 GGATGCTTCC AATCTGGATT CAATGAGGAG ACTTGCCTGG TGAAAATCAT CACTGGTCTT 420 TTGGAGTTTG AGGTATACCT AGAGTACCTC CAGAACAGAT TTGAGAGTAG TGAGGAACAA 480 GCCAGAGCTG TCCAGATGAG TACAAAAGTC CTGATCCAGT TCCTGCAGAA AAAGGCAAAG 540 AATCTAGATG CAATAACCAC CCCTGACCCA ACCACAAATG CCAGCCTGCT GACGAAGCTG 600 CAGGCACAGA ACCAGTGGCT GCAGGACATG ACAACTCATC TCATTCTGCG CAGCTTTAAG 660 GAGTTCCTGC AGTCCAGCCT GAGGGCTCTT CGGCAAATGT AGCATGGGCA CCTCAGATTG 720 TTGTTGTTAA TGGGCATTCC TTCTTCTGGT CAGAAACCT G TCCACTGGGC ACAGAACTTA 780 TGTTGTTCTC TATGGAGAAC TAAAAGTATG AGCGTTAGGA CACTATTTTA ATTATTTTTA 840 ATTTATTAAT ATTTAAATAT GTGAAGCTGA GTTAATTTAT GTAAGTCATA TTTTATATTT 900 TTAAGAAGTA CCACTTGAAA CATTTTATGT ATTAGTTTTG AAATAATAAT GGAAAGTGGC 960 TATGCAGTTT GAATATCCTT TGTTTCAGAG CCAGATCATT TCTTGGAAAG TGTAGGCTTA 1020 CCTCAAATAA ATGGCTAACT TTATACATAT TTTTAAAGAA ATATTTATAT TGTATTTATA 1080 TAATGTATAA ATGGTTTTTA TACCAATAAA TGGCATTTTA AAAAATTC 1128

SEQ ID NO: 43 Sequence length: 1589 Sequence type: Nucleic acid Sequence type: cDNA to mRNA sequence GAATTCCTCT GGTCCTCATC CAGGTGCGCG GGAAGCAGGT GCCCAGGAGA GAGGGGATAA 60 TGAAGATTCC ATGCTGCCAC ACGACGACGAAGACGAAGACGCAAGATAGAGA 120A GGATGAAACT GCAGTCGCGG CGTGGGTAAG 240 AGGAACCAGC TGCAGAGATC ACCCTGCCCA ACACAGACTC GGCAACTCCG CGGAAGACCA 300 GGGTCCTGGG AGTGACTATG GGCGGTGAGA GCTTGCTCCT GCTCCAGTTG CGGTCATCAT 360 GACTACGCCC GCCTCCCGCA GACCATGTTC CATGTTTCTT TTAGGTATAT CTTTGGACTT 420 CCTCCCCTGA TCCTTGTTCT GTTGCCAGTA GCATCATCTG ATTGTGATAT TGAAGGTAAA 480 GATGGCAAAC AATATGAGAG TGTTCTAATG GTCAGCATCG ATCAATTATT GGACAGCATG 540 AAAGAAATTG GTAGCAATTG CCTGAATAAT GAATTTAACT TTTTTAAAAG ACATATCTGT 600 GATGCTAATA AGGAAGGTAT GTTTTTATTC CGTGCTGCTC GCAAGTTGAG GCAATTTCTT 660 AAAATGAATA GCACTGGTGA TTTTGATCTC CACTTATTAA AAGTTTCAGA AGGCACAACA 720 ATACTGTTGA ACTGCACTGG CCAGGTTAAA GGAAGAAAA C CAGCTGCCCT GGGTGAAGCC 780 CAACCAACAA AGAGTTTGGA AGAAAATAAA TCTTTAAAGG AACAGAAAAA ACTGAATGAC 840 TTGTGTTTCC TAAAGAGACT ATTACAAGAG ATAAAAACTT GTTGGAATAA AATTTTGATG 900 GGCACTAAAG AACACTGAAA AATATGGAGT GGCAATATAG AAACACGAAC TTTAGCTGCA 960 TCCTCCAAGA ATCTATCTGC TTATGCAGTT TTTCAGAGTG GAATGCTTCC TAGAAGTTAC 1020 TGAATGCACC ATGGTCAAAA CGGATTAGGG CATTTGAGAA ATGCATATTG TATTACTAGA 1080 AGATGAATAC AAACAATGGA AACTGAATGC TCCAGTCAAC AAACTATTTC TTATATATGT 1140 GAACATTTAT CAATCAGTAT AATTCTGTAC TGATTTTTGT AAGACAATCC ATGTAAGGTA 1200 TCAGTTGCAA TAATACTTCT CAAACCTGTT TAAATATTTC AAGACATTAA ATCTATGAAG 1260 TATATAATGG TTTCAAAGAT TCAAAATTGA CATTGCTTTA CTGTCAAAAT AATTTTATGG 1320 CTCACTATGA ATCTATTATA CTGTATTAAG AGTGAAAATT GTCTTCTTCT GTGCTGGAGA 1380 TGTTTTAGAG TTAACAATGA TATATGGATA ATGCCGGTGA GAATAAGAGA GTCATAAACC 1440 TTAAGTAAGC AACAGCATAA CAAGGTCCAA GATACCTAAA AGAGATTTCA AGAGATTTAA 1500 TTAATCATGA ATGTGTAACA CAGTGCCTTC AATAAATGGT ATAGCAAATG TTTTGACATG 1560 AAAAAAGGAC AATTTCAAAA AAATAAAAT 1589

SEQ ID NO: 44 Sequence length: 1639 Sequence type: Nucleic acid Sequence type: cDNA to mRNA Sequence ACAAACTTTC AGAGACAGCA GAGCACACAA GCTTCTAGGA CAAGAGCCAG GAAGAAACCA 60 CCGGAAGGAA CCATCGTACTCGTGTGTAAAC ATGACTTCCA AGCTGGCCGTGAGGTCCTTTCC GCAGCTGACGAGTAGAGCTAGCT TCCAAACCTT TCCACCCCAA ATTTATCAAA 240 GAACTGAGAG TGATTGAGAG TGGACCACAC TGCGCCAACA CAGAAATTAT TGTAAAGCTT 300 TCTGATGGAA GAGAGCTCTG TCTGGACCCC AAGGAAAACT GGGTGCAGAG GGTTGTGGAG 360 AAGTTTTTGA AGAGGGCTGA GAATTCATAA AAAAATTCAT TCTCTGTGGT ATCCAAGAAT 420 CAGTGAAGAT GCCAGTGAAA CTTCAAGCAA ATCTACTTCA ACACTTCATG TATTGTGTGG 480 GTCTGTTGTA GGGTTGCCAG ATGCAATACA AGATTCCTGG TTAAATTTGA ATTTCAGTAA 540 ACAATGAATA GTTTTTCATT GTACCATGAA ATATCCAGAA CATACTTATA TGTAAAGTAT 600 TATTTATTTG AATCTACAAA AAACAACAAA TAATTTTTGA ATATAAGGAT TTTCCTAGAT 660 ATTGCACGGG AGAATATACA AATAGCAAAA TTGGGCCAAG GGCCAAGAGA ATATCCGAAC 720 TTTAATTTCA GGAATTGAAT GGGTTTGCTA GAATGTGAT A TTTGAAGCAT CACATAAAAA 780 TGATGGGACA ATAAATTTTG CCATAAAGTC AAATTTAGCT GGAAATCCTG GATTTTTTTC 840 TGTTAAATCT GGCAACCCTA GTCTGCTAGC CAGGATCCAC AAGTCCTTGT TCCACTGTGC 900 CTTGGTTTCT CCTTTATTTC TAAGTGGAAA AAGTATTAGC CACCATCTTA CCTCACAGTG 960 ATGTTGTGAG GACATGTGGA AGCACTTTAA GTTTTTTCAT CATAACATAA ATTATTTTCA 1020 AGTGTAACTT ATTAACCTAT TTATTATTTA TGTATTTATT TAAGCATCAA ATATTTGTGC 1080 AAGAATTTGG AAAAATAGAA GATGAATCAT TGATTGAATA GTTATAAAGA TGTTATAGTA 1140 AATTTATTTT ATTTTAGATA TTAAATGATG TTTTATTAGA TAAATTTCAA TCAGGGTTTT 1200 TAGATTAAAC AAACAAACAA TTGGGTACCC AGTTAAATTT TCATTTCAGA TAAACAACAA 1260 ATAATTTTTT AGTATAAGTA CATTATTGTT TATCTGAAAT TTTAATTGAA CTAACAATCC 1320 TAGTTTGATA CTCCCAGTCT TGTCATTGCC AGCTGTGTTG GTAGTGCTGT GTTGAATTAC 1380 GGAATAATGA GTTAGAACTA TTAAAACAGC CAAAACTCCA CAGTCAATAT TAGTAATTTC 1440 TTGCTGGTTG AAACTTGTTT ATTATGTACA AATAGATTCT TATAATATTA TTTAAATGAC 1500 TGCATTTTTA AATACAAGGC TTTATATTTT TAACTTTAAG ATGTTTTTAT GTGCTCTCCA 1560 AATTTTTTTT ACTGTTTCTG ATTGTATGGA AATATAAAAG TAAATATG AA ACATTTAAAA 1620 TATAATTTGT TGTCAAAGT 1639

SEQ ID NO: 45 Sequence length: 5961 Sequence type: Nucleic acid Sequence type: Genomic DNA sequence AGCAAATGAT CAATGTGCTT TGTGAATGAA GAGTCAACAT TTTACCAGGG CGAAGTGGGG 60 AGGTACAAAATTGTCCACATCCTTGAATG GTGTGTGAAGTA AAAGTGCCCACACAGAATCCCACAGAGAATCCCACAGAGAATCCCACAGAGAATCCA CTTAGTTATT AATACAAACT 240 ATCATCCCTG CCTATCTGTC ACCATCTCAT CTTAAAAAAC TTGTGAAAAT ACGTAATCCT 300 CAGGAGACTT CAATTAGGTA TAAATACCAG CAGCCAGAGG AGGTGCAGCA CATTGTTCTG 360 ATCATCTGAA GATCAGCTAT TAGAAGAGAA AGATCAGTTA AGTCCTTTGG ACCTGATCAG 420 CTTGATACAA GAACTACTGA TTTCAACTTC TTTGGCTTAA TTCTCTCGGA AACGATGAAA 480 TATACAAGTT ATATCTTGGC TTTTCAGCTC TGCATCGTTT TGGGTTCTCT TGGCTGTTAC 540 TGCCAGGACC CATATGTAAA AGAAGCAGAA AACCTTAAGA AATATTTTGT AAGTATGACT 600 TTTTAATAGT ACTTGTTTGT GGTTGAAAAT GACTGAATAT CGACTTGCTG TAGCATCTCT 660 GATAGGCTGT CATCTCTTGT AGGCAGTCAT TTTGAGATTT GGTGTTATTT TGTTAATTAT 720 TGACTAGATG AGTTCCTTGA CTAAATAATC TAGATATTGT TTTAACCTTC TGCTCAGTTT 780 GTATAGAGAC TTAAAAGGGA TTTATGAATT TTCCAAAAGA TGGGCATAAT ATGGGTATGA 840 AGCATAATGA TGTTAATAAT TTTGTGGTGG GAACTCATTC AGTTGTGATA GTCAAGGAGT 900 ATGCAGATTG AAAAAAATGA TTGGTTATTA GTTTTTGACT TCTCAGACTC TAAGGTCAAG 960 ATTAGCATTA AAAAGGTAAT AGGAAATGTT TACAAATTAA AGTCAAAAAG GTCCTTAAAG 1020 CTTTGGCTTA AAAAAATAAC TGATAGGTGA TTTTCTCCAA AAAGTGATTT CAACATTCTG 1080 CTTCTCTATC TATATTACTT GTGAAGTATT CCGGAACTTC GTTGCTCACT GGGATTTTGG 1140 AAGAATTATG ATTCTGGCTA AGGAATGTTT AAAAATTTTA AGTGAATTTT TTGAGTTTCT 1200 TTTAAAATTT TATTGATGGT TAATGAAAAG TTTTTACATT TTAAATATTT CATTATTTGT 1260 TTAAAACTTA GCTGTTATAA TTATAGCTGT CATAATAATA TTCAGACATT CACAATTGAT 1320 TTTATTCTTA CAACACAAAA TCAAATCTCA CACACACACA CACACACACA CACTCGCACA 1380 TGTTTGGAAC TATCTTTTAA AGCTCGTATA ATAATACCCT ACAGGAAGGC ACAGTAGATG 1440 TAATAGAAAC CTGTACCATT GGGGGGCAGT ATTTTATAGT GGGGTGGCTT TGCTGTTTTT 1500 TGTTTTTGTA TTTTTTAGCC TAGCTTGAAA ATACTTTCTT TAGCTTACTA TAGTTTTTGG 1560 GACCTTTGGA GTATCAGCTT TGTTGAGCTC ATTTGTGACA TTGCAATTT A ATGGTTATAT 1620 TGGGAAATAA AAAAGCTAAA AGAACATAAT AGTCTTTGTC TATATCTCAC ATAAGCCTTT 1680 TGGGAATACT TATTGTTAGA ACTAAGCAGA AGAGTTGAAA AGGAAATCAG TGAATATTGT 1740 CACATCTGAG TTCAATGAAA CTTGAAATAT ATTTTTAAGG CAATTTATGG GCTAATTGTA 1800 AACCAATTTT TTCTTTTTTT TTTTTAGAAT GCAGGTCATT CAGATGTAGC GGATAATGGA 1860 ACTCTTTTCT TAGGCATTTT GAAGAATTGG AAAGAGGTAA GCTGAATATT CCCATTTGGC 1920 TAATTTTCCT GTTGCTTGCT TTCTGATGGA TAAATTCACA TCATCCTCTG TTTGTGCTCT 1980 TTCCTTCCAA GGAGAGTGAC AGAAAAATAA TGCAGAGCCA AATTGTCTCC TTTTACTTCA 2040 AACTTTTTAA AAACTTTAAA GATGACCAGA GCATCCAAAA GAGTGTGGAG ACCATCAAGG 2100 AAGACATGAA TGTCAAGTTT TTCAATAGCA ACAAAAAGAA ACGAGATGAC TTCGAAAAGC 2160 TGACTAATTA TTCGGTGAGG CTATTTAAAT TCTTTCTTTG GTTTCATTGC CGAGGGTCTT 2220 GCAAAGCATT TATTCTCCAG AAAGTAGACA TTAGCTATTT AACAGTTGCT AAAGCTATGA 2280 ACTCAACTCA TGGCTGAAAC TCTACCTTAC TATTTCCATT CGTGTTTGGG TGACTTTGCA 2340 AAGCCAGTAA GAGAATCGCT GAAGTATGTA ATGTAGAGAA ATGCTGGCAT TGTAACTATT 2400 GCGTAAAGAC AGGTGAGTTG ACAAATTCCA GTGAAGAGGA AGTAGGTGAG GAAG AAGCAG 2460 GGAGTACTGA GAAGCAGTTC TCTCATTGTC CCTTGCTCAT ATGATGGAAA TTCTCTTACT 2520 TTGAATGAGA GGCTGTCTGT CTTAATGGAA AGAGCAGTGG GAGGAGCTGA GAAGATGTGT 2580 GTTCTCCTCC CAACTCAGCC ACCAAGGAAC TGTGATGAAT CACATGGCTG GCTGGGCTCA 2640 GTTTCCTCAT CTTAAAAGGA AACTGTTAGG TTCACTGTAT AAGTTTGATG ACCTTCTTTG 2700 CTCCAAAACT CTACAATGCA AAGAATAGAA AATGAGAATG AGATAGAAGA AAGCTACAGT 2760 CTTTGAATAG GTACCAGGGA CACCCCACTG CAAGTCTCTA GCCAACCTAT CAGATTGTAC 2820 TGCCCAATTA GAAGCAAGAA TGGTTGCTGT TTGTTTGTTT TTAGGGAAAA ATAGATAGAA 2880 TTTATACCTT ATGAAAAGAT TGTTCTATCA ACTCTCTATC AACTTTCAGA ATATCTCAGC 2940 TGGAGAACTC CTTAGACTCC TAAGTCTTAC CTCATGAACT TGTATCTTTA AGTTATGGCT 3000 TCTATAAACA GAAAGATAAC GTTGAGGCAT AAAGACAAAT CATGTTTTTC AGAATGTTTT 3060 CTAGAAGACA AAGGCCTCTA GATTCCTTTG GGGTTGACTT TGATATAAAT GGGCTCAAAT 3120 GAGAGGGACC AGGGTCTTCA AGCTAGCATT TGTGTTCTTA GGATATGTGC TCAGCTTTCA 3180 CTATTGCTGG GCCTGCCTCT CACTCCTCTC ATGTAAGCCC CCAGAAACAG AAAGGAGAGA 3240 CATGGCAACA GGTCTCCTTT GGTTATAAAC TAGACACTCA GCACTTGTTT CTAATCCAGT 3300 GGTGCCCCTG GCTTACTGTT CAGTCCTGGA TAAGTCTCTT AGTTTCTTGG TGATGATTTG 3360 AACATTGGAA AGTAAAATCT GTCACTTGCA AACACACAGC TTGTCGAAAA TTTTTTCTAC 3420 TCTGCAGGAA CTGGGCCTTA AAAAAATGAA AAAAAATCTG TGGTTTCTTC CTTCTGGAAG 3480 CTACAAACCT CCTGTTTCTT GATGGGCAAT CTTGAGTGAG CTCTATTAAT TATTATTCTC 3540 TTTGGCTCAG TTGCTAAGCT ATTTTATGCA TGTTATGCCC TTTGACAATT AGTCTTTAGC 3600 TGTAATCCCC CAGCCATCCT CAGAAATGTG GTGAGGTAGC CATAGTGTTC CCAAGATTAG 3660 AAAAATGTAA TGGCAGAGCC AAGAGGAAGG TAAATGGTCC ACATCTTATG AAGCATCATC 3720 TAAATGGCCC TATTGGTTAG AGTGAGGAGA TGCAAGTAGT TCAATTTGCT TGCCTAGAAG 3780 GCAGGGTACT GGAAAAGTTG TTGCAATTCT TAATTTTAAA CTTTATATAT CAGTAAGCCA 3840 TATATAAATA TGATTGGGGG TGTTTATTTT AAAATCTATT ATGGAAATTG AGAGACTGAC 3900 CTAATCTGGG AGAAATTAAA AATTACAGTT TTCACTCGTT TTGGATTTGG TGTTTTCTAG 3960 GGTACCTAAC CTAGATCAGT GGTTCTCAAA CTTAGGTGGA TGTCAGAATC ACCTGGGGAG 4020 CTTAGTGAAT GCACAGGGCA CAGTCCTTCC ACTTCATGCA CCTGGATCTC TGAGGTCTTT 4080 GACAGGTTTC CGGATTAATC TGCTATGCAC AACAGTGAGA ATCATTGACC TATAGTTACT 4140 CATTTGATGC ATACAGGAAA GACTGAAGTA TAAAGTGATA TAATTGGTAG ATTGATGATA 4200 GAGAGGTCAT AGAAACAGTC TCATCCTCCT TTAGATGAGA AAATAGAAGT TCAGAGAGGT 4260 TAAGTAGCTG GCTCAAGGTC AGAATTATTG CATGCATGAG ATTCAAACCC ACCTTTTTAT 4320 GCTGACTCCA CAACCAGGAG TCTTTTCACT ATATAATTTC AAGAATTCTA TAGAAGTAGA 4380 TTTAAAGATA TGTGATGGAC TCCACCACAT TATAGCACAA CTAGAAATGT AATTGTAATT 4440 TTTAGCTTCA ACTGCTGAAG AAGTAAATAT TGTATATTAA GGTAATACGG TCCATTTTTT 4500 AAAGGAATAC TTTTATTTTC ACTGACCATC ATGACATTAG CAGAATATCC TGATGGCTTA 4560 TATGCCTGAA ATTAATTTTG CTCTTTTCTT TCCCGATAGG TAACTGACTT GAATGTCCAA 4620 CGCAAAGCAA TACATGAACT CATCCAAGTG ATGGCTGAAC TGTCGCCAGC AGCTAAAACA 4680 GGGAAGCGAA AAAGGAGTCA GATGCTGTTT CGAGGTCGAA GAGCATCCCA GTAATGGTTG 4740 TCCTGCCTGC AATATTTGAA TTTTAAATCT AAATCTATTT ATTAATATTT AACATTATTT 4800 ATATGGGGAA TATATTTTTA GACTCATCAA TCAAATAAGT ATTTATAATA GCAACTTTTG 4860 TGTAATGAAA ATGAATATCT ATTAATATAT GTATTATTTA TAATTCCTAT ATCCTGTGAC 4920 TGTCTCACTT AATCCTTTGT TTTCTGACTA ATTAGGCAAG GCTATGTGAT TACAAGGCTT 4980 TATCTC AGGG GCCAACTAGG CAGCCAACCT AAGCAAGATC CCATGGGTTG TGTGTTTATT 5040 TCACTTGATG ATACAATGAA CACTTATAAG TGAAGTGATA CTATCCAGTT ACTGCCGGTT 5100 TGAAAATATG CCTGCAATCT GAGCCAGTGC TTTAATGGCA TGTCAGACAG AACTTGAATG 5160 TGTCAGGTGA CCCTGATGAA AACATAGCAT CTCAGGAGAT TTCATGCCTG GTGCTTCCAA 5220 ATATTGTTGA CAACTGTGAC TGTACCCAAA TGGAAAGTAA CTCATTTGTT AAAATTATCA 5280 ATATCTAATA TATATGAATA AAGTGTAAGT TCACAACTAC TTATGCTGTG TTGGACTTTT 5340 TCTAAGTGAG ACCTGGAGTG AAAGAACTAC CTATTAATGA ATTAGTAGGG AGGGGAGTCT 5400 TCTTAGCTGT GAAAATTTTA GAGTTGCATT TGGTTCCATT AAATGTGGTA TTTCTTTCCA 5460 CTAGCATTTT GTTGGCTTTC GCTTTTCCAG TTAGCAGCTC TTTGAATTAT CTTTCTAAGA 5520 TACAGATTTA ATTATGTCAC TATTCAATTC AGAGGTTCTG CTATGGAATG TAGTTTAAAC 5580 TGCTTAGCTT GGCACACAGA GATTTATTTC TAGCCCCTTC TCCACCTTCC TATTTCCTCC 5640 TTCGTTTCAG AATCTTCCTC TCCCTCATCC AATGCTGGCA AACACCAGTG GGGGTGGAGT 5700 AGTGGGTGTA AGCTCTAGGG AGAAGGCTTG GATTGGAATC CAAGTTATTC CATTACAAGT 5760 AGTGTGACCT TTAATACATT ATGTATATTG TCTAAGTTTC AGCTTTATTG TCTGAAAAAG 5820 AAAAATAATT G TGTGTTCCT CATAATATTG TGGTACGAAT TGATTCTTTC ACTCAAGAAA 5880 TATTTACTGG AGTACCTACT ACATGCCTGG TGCTGTTGTA GACCTTGAGA TACCTTACTC 5940 AAGCAAAACA GCCAAGGATC C 5961

SEQ ID NO: 46 Sequence length: 1761 Sequence type: Nucleic acid Sequence type: cDNA to mRNA Sequence TTGCCGATCC GCCGCCCGTC CACACCCGCC GCCAGCTCAC CATGGATGAT GATATCGCCG 60 CGCTCGTCGT CGAGGCAGCGC CCCCGGCATCC CCCCGGCATCC GCCCCGCATCCG AGGCCCAGAG CAAGAGAGGC ATCCTCACCC 240 TGAAGTACCC CATCGAGCAC GGCATCGTCA CCAACTGGGA CGACATGGAG AAAATCTGGC 300 ACCACACCTT CTACAATGAG CTGCGTGTGG CTCCCGAGGA GCACCCCGTG CTGCTGACCG 360 AGGCCCCCCT GAACCCCAAG GCCAACCGCG AGAAGATGAC CCAGATCATG TTTGAGACCT 420 TCAACACCCC AGCCATGTAC GTTGCTATCC AGGCTGTGCT ATCCCTGTAC GCCTCTGGCC 480 GTACCACTGG CATCGTGATG GACTCCGGTG ACGGGGTCAC CCACACTGTG CCCATCTACG 540 AGGGGTATGC CCTCCCCCAT GCCATCCTGC GTCTGGACCT GGCTGGCCGG GACCTGACTG 600 ACTACCTCAT GAAGATCCTC ACCGAGCGCG GCTACAGCTT CACCACCACG GCCGAGCGGG 660 AAATCGTGCG TGACATTAAG GAGAAGCTGT GCTACGTCGC CCTGGACTTC GAGCAAGAGA 720 TGGCCACGGC TGCTTCCAGC TCCTCCCTGG AGAAGAGCT A CGAGCTGCCT GACGGCCAGG 780 TCATCACCAT TGGCAATGAG CGGTTCCGCT GCCCTGAGGC ACTCTTCCAG CCTTCCTTCC 840 TGGGCATGGA GTCCTGTGGC ATCCACGAAA CTACCTTCAA CTCCATCATG AAGTGTGACG 900 TGGACATCCG CAAAGACCTG TACGCCAACA CAGTGCTGTC TGGCGGCACC ACCATGTACC 960 CTGGCATTGC CGACAGGATG CAGAAGGAGA TCACTGCCCT GGCACCCAGC ACAATGAAGA 1020 TCAAGATCAT TGCTCCTCCT GAGCGCAAGT ACTCCGTGTG GATCGGCGGC TCCATCCTGG 1080 CCTCGCTGTC CACCTTCCAG CAGATGTGGA TCAGCAAGCA GGAGTATGAC GAGTCCGGCC 1140 CCTCCATCGT CCACCGCAAA TGCTTCTAGG CGGACTATGA CTTAGTTGCG TTACACCCTT 1200 TCTTGACAAA ACCTAACTTG CGCAGAAAAC AAGATGAGAT TGGCATGGCT TTATTTGTTT 1260 TTTTTGTTTT GTTTTGGTTT TTTTTTTTTT TTTGGCTTGA CTCAGGATTT AAAAACTGGA 1320 ACGGTGAAGG TGACAGCAGT CGGTTGGAGC GAGCATCCCC CAAAGTTCAC AATGTGGCCG 1380 AGGACTTTGA TTGCACATTG TTGTTTTTTT AATAGTCATT CCAAATATGA GATGCATTGT 1440 TACAGGAAGT CCCTTGCCAT CCTAAAAGCC ACCCCACTTC TCTCTAAGGA GAATGGCCCA 1500 GTCCTCTCCC AAGTCCACAC AGGGGAGGTG ATAGCATTGC TTTCGTGTAA ATTATGTAAT 1560 GCAAAATTTT TTTAATCTTC GCCTTAATAC TTTTTTATTT TGTTTTAT TT TGAATGATGA 1620 GCCTTCGTGC CCCCCCTTCC CCCTTTTTGT CCCCCAACTT GAGATGTATG AAGGCTTTTG 1680 GTCTCCCTGG GAGTGGGTGG AGGCAGCCAG GGCTTACCTG TACACTGACT TGAGACCAGT 1740 TGAATAAAAG TGCACACCTT A 1761

[0092] SEQ ID NO: 47 SEQ Length: 4100 sequence type: nucleic acid sequence of the Type: Genomic DNA sequence ACCTCTTCCT CAACTCACTT CTCTCTACTC TCACTTTTTT TTTTCTTCTT CTTTTTTTTT 60 TTTTTTTTTT TTTTTTTTTT TGCAAAAGAA GGGGGTAAAA AAATGCTGCA CTGTCGGGCG 120 AGGCCGGTGA GTGAGCGAGC CGGAGCCAAT CAGCGCCCGC CGTTCCGAAA TTGCCTTTTA 180 TGGCTCGAGT GGCCGCTGTG GCGTCCTATA AAACCCGGCG GCGCAACGCG CGCCACTGTC 240 GAGTCCGCGT CCACCCGCGA GTACAACCTC CTTGCAGCTC CTCCGTCCCG GTCCACACCC 300 GCCACCAGGT AAGCAGGGAC GTCGGGCCCA GCGGGCCCCA ACTTTACCTT GGCCACTACC 360 TCGCTGCAGG ATCGTGAGGA ACACTCAGAA GGGACACCGT AGAGGGGTGG AGCGTGGTAC 420 CGGGCCGCGG AGCAGCTGGC AAAGCTTAAC TTTCCCGGCC CCTAGGGTGT AGAGTGTTTG 480 CAGTCGTATT CCCGCGGTGT GGACACTCGT GGGTACGCTC CTGCTTGGTG CGCGGGGCTT 540 GGGGACACAC TAGAGTCGCG GTGTGGGCAT TTGGAGAGCC GGTGCGGCTG CGGGTGTTAA 600 GCCGCATCTG TCCACCTTGA GGGGCAACAG TATTGGGGTC AGGCGTTACA ATCACGCTTT 660 GATGGCCTAT GGGTCTTTGT CCAAACCGGT TTTGCCATTC GGCTTGGCGG GCGCGGCGGG 720 GCCGCTCGGC CGGGTGGGGG CTGGGATGCC ATTGCGCGTG CGCGCTCTAT CACTGGGCAT 780 TGGGGCCGTG CGCGCTGGGG AGGGAACTCT TCCTCTCCCC CTCTTCCGAG TTAAGAGTTG 840 CGCGTGCGTA TTGAGACTAG GAGCGCGGCC GCCCCGGGTT GGGCGAGGGC GGGCCGTCCA 900 CCGGAAGGGG CGGGGTCGTA GCGGCTAGGC GCCTGCTCGC GCTTCCTGCT GGGTGTGGTC 960 GCCTCCCGCG CGCGCACTAG CCGCCCGTCG CCTCAGTGTA GGCGGGCCTG TGCCCGTTTG 1020 GGGAGGGGCG GAGGCCTGGC TTCCTGCCGT GGGTCCGCCT CCGGGCCAGC GTTTGCCTTT 1080 TATGGTAATA ATGCGGCTGT CCTGCGCTTC CTTTGTCCCC TGAGCTTGGG CGCGCCCCTG 1140 GCGGCTCGAG GCCGCGGCTC GCCGGAAGTG GGCAGGGCGG CAGCGGCTGC TCTTGGCGGC 1200 TCCGCGGTGA CCATAGCCCT CTTTTGTGCC TTGATAGTTC GCCATGGATG ACGATATCGC 1260 TGCGCTCGTC GTCGACAACG GCTCCGGCAT GTGCAAGGCC GGCTTCGCGG GCGACGATGC 1320 TCCCCGGGCC GTCTTCCCCT CCATCGTGGG CCGCCCTAGG CACCAGGTAA GTGACCCTTT 1380 ACTTTGGGAG TGGCAGCCCT AGGGTTTTCT TGGGGGTCGA TGCCAGTGCT GAGAACGTTG 1440 TTCTCCTCCG CAGGGTGTGA TGGTGGGTAT GGGTCAGAAG GACTCCTACG TGGGCGACGA 1500 GGCCCAGAGC AAGAGAGGCA TCCTGACCCT GAAGTACCCC ATTGAACACG GCATTGTAAC 1560 CAACTGGGAC GATATGGAGA AGATTTGGCA CCACACTTTC TACAATGAG C TGCGTGTGGC 1620 CCCTGAGGAG CACCCTGTGC TGCTCACCGA GGCCCCTCTG AACCCTAAGG CCAACCGTGA 1680 AAAGATGACC CAGGTCAGTA TCCTGGGTGA CCCTCCCCTT CTTATTGGGT CAACTTCTCA 1740 GCACGCCCTT CTCTAATTGT CTTTCTTCTG CCATGTCCCA TAGGACTCTC TTCTATGAGC 1800 TGAGTCTCCC TTGGAACTTT GCAGTTTCTG CTCTTTCCCA GATGAGGTCT TTTTTTTCTC 1860 TCGATCGCCT TTCTGACTAG GTGTTTTAAA CCCCTACAGT GCTGTGGGTG TAGGTACTAA 1920 CAATGGCTCG TGTGACAAAG CTAATGAGGC TGGTGATAAA TGGCCTTGGA GTGTGTATTC 1980 AGTAGATGAC AGTAGGTCTA AATGGAGCCC CTGTCCTGAT ACTCCCAGCA CACTTAACTT 2040 AGCTGTGTTC TTGCCCTCTT TGCATGTCTC ACTCAAATCT ATCCTTACAG TCTCACCTGC 2100 CCTGAGTGTT TCTTGTGGCT TTAGGAGCTT GACAATACTG TATTCCTTTC TCTACAGATC 2160 ATGTTTGAGA CCTTCAACAC CCCAGCCATG TACGTAGCCA TCCAGGCTGT GTTGTCCCTG 2220 TATGCCTCTG GTCGTACCAC TGGCATTGTG ATGGACTCCG GAGACGGGGT CACCCACACT 2280 GTGCCCATCT ATGAGGGTTA CGCGCTCCCT CATGCCATCC TGCGTCTGGA CCTGGCTGGC 2340 CGGGACCTGA CAGACTACCT CATGAAGATC CTGACCGAGC GTGGCTACAG CTTCACCACC 2400 ACAGCTGAGA GGGAAATCGT GCGTGACATT AAAGAGAAGC TGTGCTATGT TGCC CTAGAC 2460 TTCGAGCAAG AGATGGCCAC TGCCGCATCC TCTTCCTCCC TGGAGAAGAG CTATGAGCTG 2520 CCTGACGGTC AGGTCATCAC TATCGGCAAT GAGCGGTTCC GATGCCCCGA GGCTCTCTTC 2580 CAGCCTTCCT TCCTGGGTAA GTTGTAGTCT CGTCCCTTCT CCATCTAAAG GTGACCAATG 2640 CTGGAGGCCA CACTGTAACT CTGATCTCTT GCTTTTCCTT TCAGGTATGG AATCCTGTGG 2700 CATCCATGAA ACTACATTCA ATTCCATCAT GAAGTGTGAC GTTGACATCC GTAAAGACCT 2760 CTATGCCAAC ACAGTGCTGT CTGGTGGCAC CACCATGTAC CCAGGCATCG CTGACAGGAT 2820 GCAGAAGGAG ATTACTGCCC TGGCTCCTAG CACCATGAAG ATCAAGGTAA GCAGCCTTAG 2880 CCTGGACCCA TAGTGGGGTG TGGTCAGCCC TGTAGTTGTA GCCAACTCTC TTGGCTTAAG 2940 GAACAACCCA GCATCCAGAA TGCTCACAAT CACTGTCTTG CTTTCTTCAG ATCATTGCTC 3000 CTCCTGAGCG CAAGTACTCT GTGTGGATTG GTGGCTCTAT CCTGGCCTCA CTGTCCACCT 3060 TCCAGCAGAT GTGGATCAGC AAGCAGGAGT ACGATGAGTC CGGCCCCTCC ATCGTGCACC 3120 GCAAATGCTT CTAGGCGGAC TGTTACTGAG CTGCGTTTTA CACCCTTTCT TTGACAAAAC 3180 CTAACTTGCG CAGAAAAAAA AAATGAGACA TTTGGCATGG CTTTATTGTT TTTTTGTTTT 3240 TTGTTTTTGT TTTTTTTAAA TTTTTTTTTT AAAAAGGTTT TTTTTTTTTG TTTTGTTTTG 3300 GCGCTTTTGA CTCAAGGATT TAAAAACTGG AACGGTGAAG GCGACCGCAG TTGGTTGGAG 3360 CAAACATCCC CCAAAGTTCT ACAATGTGGC TGAGGACTTT GATTGTACAT TGTTTTTTGT 3420 TTTTGGTTTT TTTAATAGTC ACTCCAAGTA TCCACGGCAT AGATGGTTAC AGGAAGTCCC 3480 TCACCCTCCC AAAAGCCACC CCCAACTCCT AAGGGGAGGA TGGCTGCATC CATGCCCTGA 3540 GTCCACACCG GGAAGGTGAC AGCATTGCTT CTGTGTAAAT TATGTACTTG CAAACATTTT 3600 TTTAAATCTT CCGCCTTAAT ACTTCATTTT TGTTTTTAAT TTCTGAATGG TCAGCCATTC 3660 GTGGCCTGCC CCTTTTTTTT GTCCCCCCAA CTTGATGTAT GAAGGCTTTG GTCTCCCTGG 3720 GAGTGGTTTG AGGTGTTGAG GCGCCAGGGC TGGCCTGTAC ACTGACGTGA GACCGTTTTA 3780 ATAAAAGTGC ACACCTTACA AACAAGTTTG TGGCTCTGTG GCTTCTACTG GGTGTGGGGA 3840 GCAGGCTGGG TGGGTGTGNA ACTCCACGTG GGGGAGGGGC AATTTAGGGT GGGCTGGCCT 3900 TGCCTGATAG CTAGTGGGAG GGCTAAAGGA TCATGATCTT TAATGAGGTC TCATAAATAC 3960 CCCACCTTCG GTCTTGAGGG AGGGTGAGGA GTTGGGAAGG TATCTCCCTG ACACCTGGCC 4020 AAGCTGGCCT CATCAACCCT ACTTTCCTCA GCCAGGGGGA GGTCCACATC CTCCCACCTG 4080 CATAGCTTGC GTCTAGAGGC 4100

SEQ ID NO: 48 Sequence length: 1892 Sequence type: Nucleic acid Sequence type: cDNA to mRNA sequence CCGGTCGAGT CGCGTCCACC CGCGAGCACA GCTTCTTTGC AGCTCCTTCGCC ATGGATGACG 120 TGGTGGGAAT GGGTCAGAAG GACTCCTATG 240 TGGGTGACGA GGCCCAGAGC AAGAGAGGTA TCCTGACCCT GAAGTACCCC ATTGAACATG 300 GCATTGTTAC CAACTGGGAC GACATGGAGA AGATCTGGCA CCACACCTTC TACAATGAGC 360 TGCGTGTGGC CCCTGAGGAG CACCCTGTGC TGCTCACCGA GGCCCCCCTG AACCCTAAGG 420 CCAACCGTGA AAAGATGACC CAGATCATGT TTGAGACCTT CAACACCCCA GCCATGTACG 480 TAGCCATCCA GGCTGTGCTG TCCCTGTATG CCTCTGGTCG TACCACAGGC ATTGTGATGG 540 ACTCCGGAGA CGGGGTCACC CACACTGTGC CCATCTACGA GGGCTATGCT CTCCCTCACG 600 CCATCCTGCG TCTGGACCTG GCTGGCCGGG ACCTGACAGA CTACCTCATG AAGATCCTGA 660 CCGAGCGTGG CTACAGCTTC ACCACCACAG CTGAGAGGGA AATCGTGCGT GACATCAAAG 720 AGAAGCTGTG CTATGTTGCT CTAGACTTCG AGCAGGAGA T GGCCACTGCC GCATCCTCTT 780 CCTCCCTGGA GAAGAGCTAT GAGCTGCCTG ACGGCCAGGT CATCACTATT GGCAACGAGC 840 GGTTCCGATG CCCTGAGGCT CTTTTCCAGC CTTCCTTCTT GGGTATGGAA TCCTGTGGCA 900 TCCATGAAAC TACATTCAAT TCCATCATGA AGTGTGACGT TGACATCCGT AAAGACCTCT 960 ATGCCAACAC AGTGCTGTCT GGTGGTACCA CCATGTACCC AGGCATTGCT GACAGGATGC 1020 AGAAGGAGAT TACTGCTCTG GCTCCTAGCA CCATGAAGAT CAAGATCATT GCTCCTCCTG 1080 AGCGCAAGTA CTCTGTGTGG ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC 1140 AGATGTGGAT CAGCAAGCAG GAGTACGATG AGTCCGGCCC CTCCATCGTG CACCGCAAGT 1200 GCTTCTAGGC GGACTGTTAC TGAGCTGCGT TTTACACCCT TTCTTTGACA AAACCTAACT 1260 TGCGCAGAAA AAAAAAAAAT AAGAGACAAC ATTGGCATGG CTTTGTTTTT TTAAATTTTT 1320 TTTAAAGTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTAA GTTTTTTTGT TTTGTTTTGG 1380 CGCTTTTGAC TCAGGATTTA AAAACTGGAA CGGTGAAGGC GACAGCAGTT GGTTGGAGCA 1440 AACATCCCCC AAAGTTCTAC AAATGTGGCT GAGGACTTTG TACATTGTTT TGTTTTTTTT 1500 TTTTTTTGGT TTTGTCTTTT TTTAATAGTC ATTCCAAGTA TCCATGAAAT AAGTGGTTAC 1560 AGGAAGTCCC TCACCCTCCC AAAAGCCACC CCCACTCCTA AGAGGAGG AT GGTCGCGTCC 1620 ATGCCCTGAG TCCACCCCGG GGAAGGTGAC AGCATTGCTT CTGTGTAAAT TATGTACTGC 1680 AAAAATTTTT TTAAATCTTC CGCCTTAATA CTTCATTTTT GTTTTTAATT TCTGAATGGC 1740 CCAGGTCTGA GGCCTCCCTT TTTTTTGTCC CCCCAACTTG ATGTATGAAG GCTTTGGTCT 1800 CCCTGGGAGG GGGTTGAGGT GTTGAGGCAG CCAGGGCTGG CCTGTACACT GACTTGAGAC 1860 CAATAAAAGT GCACACCTTA CCTTACACAA AC 1892

SEQ ID NO: 49 Sequence length: 2246 Sequence type: Nucleic acid Sequence type: cDNA to mRNA sequence GGCGCTCTGG TGCTCACTGA GGAAGCTTCC TTAGGTGTAC CGATCTTATAT GATTGAGCCC 60 TTGGAGCAGTTCTCGACTGTCTG GACTGTCT ATCTTGATTCT CTCTTACAGTACT CTTAGAATAG TGTAGTTAGT TAGGGGCCTT 240 CAAAGAAGAA AGAAGAAGCG ATTGGCGCGG AGAGATGCTG GAGGTGTCAG TTTCTATGCT 300 AGAGTAGGGT AGTGAAACAA TCCCCAGCCA AACCTTTCCG GGGGGCGCAG GTTGCCCACA 360 GGAGGTCGAC TTGCCGGCGC TGTCCTTCGC GCCGAGCTCC CTCCATCCTT CTTTCCGTCT 420 GCTGAGACGC AAGGTTGCGG CGCGCACGCT GAGCAGCGCA CTGACTGCCG CGGGCTCCGC 480 TGGGCGATTG CAGCCGAGTC CGTTTCTCGT CTAGCTGCCG CCGCGGCGAC CTGCCTGGTC 540 TTCCTCCCGG ACGCTAGCGG GTTGTCAACT ATTACCTGCA AGCATAGGCC AACGAACACC 600 TTCTTTCCAA ATTAATTGGA ATGAAACAAT TCTGTTAACT TCCTAATTCT CAGTTTGAAA 660 CTCTGGTTGC TTAAGCCTGA AGCAATCATG GTGAACCTTG GCAACGCGGT GCGCTCGCTC 720 CTGATGCACC TAATCGGCCT ATTGGTTTGG CAATTCGAT A TTTCCATAAG TCCAGTAGCA 780 GCTATAGTAA CTGACACTTT TAATTCCTCC GATGGTGGAC GCTTGTTTCA ATTCCCGGAC 840 GGGGTACAAA ACTGGCCAGC ACTTTCAATC GTCGTGATTA TAATCATGAC AATAGGGGGC 900 AACATTCTTG TTATCATGGC AGTAAGCATG GAGAAGAAAC TGCACAATGC AACCAATTAC 960 TTCTTAATGT CCCTAGCCAT TGCTGATATG CTGGTGGGAC TACTTGTCAT GCCCCTGTCC 1020 CTGCTTGCTA TTCTTTATGA TTATGTCTGG CCTTTACCTA GATATTTGTG CCCCGTCTGG 1080 ATTTCACTAG ATGTGCTATT TTCAACTGCG TCCATCATGC ACCTCTGCGC CATATCGCTG 1140 GACCGGTATG TAGCAATACG TAATCCTATT GAGCATAGCC GGTTCAATTC GCGGACTAAG 1200 GCCATCATGA AGATTGCCAT CGTTTGGGCA ATATCAATAG GAGTTTCAGT TCCTATCCCT 1260 GTGATTGGAC TGAGGGACGA AAGCAAAGTG TTCGTGAATA ACACCACGTG CGTGCTCAAT 1320 GACCCCAACT TCGTTCTCAT CGGGTCCTTC GTGGCATTCT TCATCCCGTT GACGATTATG 1380 GTGATCACCT ACTTCTTAAC GATCTACGTC CTGCGCCGTC AAACTCTGAT GTTACTTCGA 1440 GGTCACACCG AGGAGGAACT GGCTAATATG AGCCTGAACT TTCTGAACTG CTGCTGCAAG 1500 AAGAATGGTG GTGAGGAAGA GAACGCTCCG AACCCTAATC CAGATCAGAA ACCACGTCGA 1560 AAGAAGAAAG AAAAGCGTCC CAGAGGCACC ATGCAAGCTA TCAACAAC GA AAAGAAAGCT 1620 TCCAAAGTCC TTGGCATTGT ATTCTTTGTG TTTCTGATCA TGTGGTGCCC GTTTTTCATC 1680 ACCAATATCC TGTCGGTTCT TTGTGGGAAG GCCTGTAACC AAAAGCTAAT GGAGAAGCTT 1740 CTCAATGTGT TTGTGTGGAT TGGCTATGTG TGTTCAGGCA TCAATCCTCT GGTGTACACT 1800 CTCTTTAATA AAATTTACCG AAGGGCTTTC TCTAAATATT TGCGCTGCGA TTATAAGCCA 1860 GACAAAAAGC CTCCTGTTCG ACAGATTCCT AGGGTTGCTG CCACTGCTTT GTCTGGGAGG 1920 GAGCTCAATG TTAACATTTA TCGGCATACC AATGAACGTG TGGCTAGGAA AGCTAATGAC 1980 CCTGAGCCTG GCATAGAGAT GCAGGTGGAG AACTTAGAGC TGCCAGTCAA CCCCTCTAAT 2040 GTGGTCAGCG AGAGGATTAG TAGTGTGTAA GCGAAGAGCA GCGCAGACTT CCTACAGGAA 2100 AGTTCCTGTA GGAAAGTCCT CCCCACCCCCCC CGTGATTTTC CTGTGAATCA TAACTAATGT 2160 AAATATTTACCT ATGACTGACAGACAGCGTTAG

SEQ ID NO: 50 Sequence length: 2217 Sequence type: Nucleic acid Sequence type: cDNA to mRNA sequence ACGCGATAAC TGTCAATATG AAAGTCACTT GATTTTTACA GAAATACAAG CTCAAATTCC 60 TGGACCGCGG CTGAGGCTACTTCTGCCTGTACGACGATGACGAGATATTCAG TGACAGAGAT GCTGTTTTAA CTTTGTGATG GCTGAACTCT 240 TGGAAGCAGC ATATTCAACC CGAGAATTAG CTGAAAGATT TTCACGGATA CAAAACTTTT 300 CTTCCTTGAA CCAGGAACAC GTTTGTGTCC CCGAATACTC AAAGTGCTTT TTTTGGCCTT 360 TGCTTCCGTG AGAACTTACA GCTCAGCCGT GGGCTCTCCC TAGCACCGTG AAGGGAGGCA 420 TAATCAAGAA GCATCACACT TCTGTAACTC TTACTATGGA AGAGGAGAAG GCAGCCAGAG 480 GAGCCACACA TGTTCTCCGC TTCAGCACGT CCTAGCGCCA GGGCACGAAG ATGAATGGTG 540 AGCCCAGGCT ATGACCCCCT AGTCTCTCCA CACTTCATCT GCTACAACTT CCGGCTTAGA 600 CATGGAAATT CTTTGTGAAG ACAATATCTC TCTGAGCTCA ATTCCAAACT CCTTAATGCA 660 ATTAGGTGAT GGCCCGAGGC TCTACCATAA TGACTTCAAC TCCAGAGATG CTTAACACTT 720 CGGAAGCATC GAACTGGACA ATTGATGCTG AAAACAGAA C CAACCTCTCC TGTGAAGGGT 780 ACCTCCCACC GACATGCCTC TCCATTCTTC ATCTCCAGGA AAAAAACTGG TCTGCTTTAT 840 TGACAACTGT CGTGATTATT CTCACCATTG CTGGAAATAT ACTGGTCATC ATGGCAGTGT 900 CCCTAGAAAA AAAGCTGCAG AATGCCACCA ACTATTTCCT GATGTCACTT GCCATAGCTG 960 ATATGCTGCT GGGTTTCCTT GTCATGCCTG TGTCCATGTT AACCATCCTG TATGGGTACC 1020 GGTGGCCTTT GCCTAGCAAG CTCTGTGCGA TCTGGATTTA CCTGGATGTG CTCTTTTCTA 1080 CGGCATCCAT CATGCACCTC TGCGCCATCT CCCTGGACCG CTATGTCGCC ATCCAGAACC 1140 CCATTCACCA CAGCCGCTTC AACTCCAGAA CCAAAGCCTT CCTGAAAATC ATTGCCGTGT 1200 GGACCATATC TGTAGGTATA TCCATGCCAA TCCCAGTCTT TGGACTACAG GATGATTCGA 1260 AGGTCTTTAA GGAGGGGAGC TGCCTGCTTG CCGATGACAA CTTTGTTCTC ATAGGCTCTT 1320 TTGTGGCATT TTTCATCCCC CTAACCATCA TGGTGATCAC CTACTTCCTG ACTATCAAGT 1380 CACTTCAGAA AGAAGCCACC TTGTGTGTGA GTGACCTCAG CACTCGAGCC AAACTAGCCT 1440 CCTTCAGCTT CCTCCCTCAG AGTTCTCTGT CATCAGAAAA GCTCTTCCAA CGGTCCATCC 1500 ACAGAGAGCC AGGCTCCTAC GCAGGCCGAA GGACGATGCA GTCCATCAGC AATGAGCAAA 1560 AGGCGTGCAA GGTGCTGGGC ATCGTGTTCT TCCTGTTTGT TGTAATGT GG TGCCCATTCT 1620 TCATCACCAA TATCATGGCC GTCATCTGCA AAGAATCCTG CAATGAAAAT GTCATCGGAG 1680 CCCTGCTCAA TGTGTTTGTC TGGATTGGTT ATCTCTCCTC AGCTGTCAAT CCACTGGTAT 1740 ATACGTTGTT CAATAAAACT TATAGGTCCG CCTTCTCAAG GTACATTCAG TGTCAGTACA 1800 AGGAAAACAG AAAGCCACTG CAGTTAATTT TAGTGAACAC TATACCAGCA TTGGCCTACA 1860 AGTCTAGTCA GCTCCAGGTG GGACAGAAAA AGAACTCACA GGAAGATGCT GAGCAGACAG 1920 TTGATGACTG CTCCATGGTT ACACTGGGGA AACAACAGTC GGAAGAGAAT TGTACAGACA 1980 ATATTGAAAC CGTGAATGAA AAGGTTAGCT GTGTGTGATG AACTGGATGC TATGGCAATT 2040 GCCAGGGCAT GTGAACAAGG TTATACCCAT GTGTGTGGGG CGGGGATAAG GAGGCTGCAA 2100 CAAATTAGAC TACTCCAGTC GACCAACGTA TAATGTCCTG ACAGCATTGG GAGCTAGGAG 2160 TGTTACGATG CTTTAATTAT TGCCAATGAG ATCTCTAAA CCATTGGC217GT

SEQ ID NO: 51 Sequence Length: 25 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence ATGGCCAAAG TTCCAGACAT GTTTG 25

SEQ ID NO: 52 Sequence Length: 25 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence GGTTTTCCAG TATCTGAAAG TCAGT 25

SEQ ID NO: 53 Sequence length: 23 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence ATGGCAGAAG TACCTAAGCT CGC 23

SEQ ID NO: 54 Sequence Length: 26 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence ACACAAATTG CATGGTGAAG TCAGTT 26

SEQ ID NO: 55 Sequence Length: 25 Sequence Type: Nucleic Acid Number of Strands: Single Strand Type of Sequence: Other Nucleic Acid Synthetic DNA Sequence ATGTACAGGA TGCAACTCCT GTCTT 25

SEQ ID NO: 56 Sequence Length: 25 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence GTTAGTGTTG AGATGATGCT TTGAC
25

SEQ ID NO: 57 Sequence length: 21 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence ATGAGCCGCC TGCCCGTCCT G 21

SEQ ID NO: 58 Sequence length: 23 Sequence type: Nucleic acid Number of strands: Single stranded Sequence type: Other nucleic acid Synthetic DNA Sequence GCGAGGCTCA AAGTCGTCTG TTG 23

SEQ ID NO: 59 Sequence Length: 23 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence ATGGGTCTCA CCTCCCAACT GCT 23

SEQ ID NO: 60 Sequence Length: 27 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence CGAACACTTT GAATATTTCT CTCTCAT 27

SEQ ID NO: 61 Sequence Length: 25 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence GCTTCTGCAT TTGAGTTTGC TAGCT 25

SEQ ID NO: 62 Sequence length: 27 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence TGGCCGTCAA TGTATTTCTT TATTAAG 27

SEQ ID NO: 63 Sequence Length: 23 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence ATGAACTCCT TCTCCACAAG CGC 23

SEQ ID NO: 64 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence GAAGAGCCCT CAGGCTGGAC TG 22

SEQ ID NO: 65 Sequence length: 28 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence ATGTTCCATG TTTCTTTTAG GTATATCT 28

SEQ ID NO: 66 Sequence length: 25 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence TGCATTTCTC AAATGCCCTA ATCCG 25

SEQ ID NO: 67 Sequence Length: 24 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence ATGACTTCCA AGCTGGCCGT GGCT 24

SEQ ID NO: 68 Sequence Length: 25 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence TCTCAGCCCT CTTCAAAAAC TTCTC 25

SEQ ID NO: 69 Sequence length: 32 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence GCATCGTTTT GGGTTCTCTT GGCTGTTACT GC 32

SEQ ID NO: 70 Sequence Length: 32 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence CTCCTTTTTC GCTTCCCTGT TTTAGCTGCT GG 32

SEQ ID NO: 71 Sequence Length: 21 Sequence Type: Nucleic Acid Number of Strands: Single Strand Sequence Type: Other Nucleic Acid Synthetic DNA Sequence ATGGATGATG ATATCGCCGC G 21

SEQ ID NO: 72 Sequence length: 33 Sequence type: Nucleic acid Number of strands: Single strand Sequence type: Other nucleic acid Synthetic DNA Sequence CTAGAAGCAT TTGCGGTGGA CGATGGAGGG GCG 33

[Brief description of drawings]

FIG. 1 is an electrophoretic diagram of a PCR reaction solution using the DNA fragments having the sequences of SEQ ID NOs: 1 to 6 as primers.

FIG. 2 is an electrophoretic diagram of a PCR reaction solution using the DNA fragments having the sequences of SEQ ID NOs: 7 to 10 as primers.

FIG. 3 is an electrophoretic diagram of a PCR reaction solution using the DNA fragments having the sequences of SEQ ID NOs: 11 and 12 as primers.

FIG. 4 is an electrophoretic diagram of a PCR reaction solution using the DNA fragments having the sequences of SEQ ID NOs: 13 to 15 as primers.

FIG. 5 is an electrophoretic diagram of a PCR reaction solution using the DNA fragments having the sequences of SEQ ID NOs: 18 to 21 as primers.

FIG. 6 is an electrophoretic diagram of a PCR reaction solution using the DNA fragments having the sequences of SEQ ID NOs: 22 to 24 as primers.

FIG. 7 is an electrophoretic diagram of a PCR reaction solution carried out using the DNA fragments having the sequences of SEQ ID NOs: 25 and 26 as primers.

FIG. 8 is an electrophoretic diagram of a PCR reaction solution using the DNA fragments having the sequences of SEQ ID NOs: 27 to 29 as primers.

FIG. 9 is an electrophoretic diagram of a PCR reaction solution using the DNA fragments having the sequences of SEQ ID NOs: 30 and 31 as primers.

FIG. 10 is an electrophoretic diagram of a PCR reaction solution using the DNA fragments having the sequences of SEQ ID NOs: 32 to 35 as primers.

[Explanation of symbols]

1 to 10, the numbers 1 to 18 represent lanes in electrophoresis, 243, 271, 310, 60.
3, 872, 1078, 1353, 100 and 600 represent the molecular weights of the molecular weight markers.

Claims (4)

[Claims] 1. A specific messenger RNA having any one of SEQ ID NOs: 1 to 35 or a sequence homologous thereto.
A primer for detecting and measuring. 2. A primer for detecting / measuring a cytokine-specific messenger RNA having any one of SEQ ID NOs: 1-29 or a sequence homologous thereto. 3. A primer for detecting / measuring β-actin-specific messenger RNA having the sequence of SEQ ID NO: 30 or SEQ ID NO: 31 or a sequence homologous thereto. 4. A primer for detecting / measuring a serotonin receptor-specific messenger RNA having any one of SEQ ID NOs: 32 to 35 or a sequence homologous thereto.