JPH07120393A - Fluorescence detection method - Google Patents
Fluorescence detection methodInfo
- Publication number
- JPH07120393A JPH07120393A JP27883693A JP27883693A JPH07120393A JP H07120393 A JPH07120393 A JP H07120393A JP 27883693 A JP27883693 A JP 27883693A JP 27883693 A JP27883693 A JP 27883693A JP H07120393 A JPH07120393 A JP H07120393A
- Authority
- JP
- Japan
- Prior art keywords
- fluorescence
- sample
- photomultiplier tube
- light
- measurement
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は多点の蛍光を測定するに
当り、蛍光発光の場より光ファイバーにより光検出素子
に導き、各点の励起光光源を順番に測定に必要時間点灯
させて測定点を切換え、可動部分なしに多点の蛍光を測
定するもので、血中の薬物濃度や免疫グロブリン濃度等
の測定を行う蛍光検出法に関し、医療分野でその官能基
特異性など高感度検出可能で多用され、対象検体数の多
数を高能率に測定可能とする蛍光検出法を提案した。BACKGROUND OF THE INVENTION The present invention, when measuring fluorescence at multiple points, guides the light from the field of fluorescence emission to a photodetector by an optical fiber, and turns on the excitation light source at each point in order for the required time for measurement. This is a method to measure fluorescence at multiple points without changing moving parts by switching the points, and it is possible to detect fluorescence with high sensitivity such as functional group specificity in the medical field regarding the fluorescence detection method that measures the concentration of drug or immunoglobulin in blood. We have proposed a fluorescence detection method, which is used frequently in the US and can measure a large number of target samples with high efficiency.
【０００２】[0002]
【従来の技術】蛍光測定法としては特開昭６３−２０８
７３３号に開示したものがあるが、殆んどの方式はこれ
らを応用した蛍光測定装置へ被測定物を運搬して測定し
測定終了後に排出する方式であり、試験片の運搬装置を
必要とする。この他、蛍光測定装置を被測定物のライン
に移動する方法もあるが、いずれも、上述の方法は機械
的な動作を伴うので、磨耗、焼付などに起因する故障が
多く好ましくない。2. Description of the Related Art A fluorescence measuring method is disclosed in JP-A-63-208.
Although there is a method disclosed in Japanese Patent No. 733, most of the methods are methods for transporting and measuring an object to be measured to a fluorescence measuring device to which these are applied, and discharging after the completion of the measurement, which requires a test piece transporting device. . In addition to this, there is also a method of moving the fluorescence measuring device to the line of the object to be measured, but both of them are not preferable because many of the above methods involve mechanical operations, and therefore there are many failures due to wear and seizure.
【０００３】[0003]
【発明が解決しようとする課題】上述したように従来技
術は、蛍光測定装置を被測定物を運搬及び排出する運搬
装置や、蛍光測定装置を被測定物に移動する装置を持た
ねばならないので、これらの移送装置を除去することに
あって、コンパクトな能率的な装置を造り、測定の信頼
性の向上を図ることを課題とした。As described above, the prior art must have a transporting device for transporting and discharging the fluorescence measurement device and a device for moving the fluorescence measurement device to the measurement object. In removing these transfer devices, it was an object to make a compact and efficient device and to improve the reliability of measurement.
【０００４】[0004]
【課題を解決するための手段】最も望ましい型式は各被
測定物毎に蛍光測定装置を持つことであるが、これは、
一般的に蛍光測定装置が微量光測定であり、高価、大容
積を要することから個別設置は無理を生ずる。近来、励
起光光源としては高輝度のＬＥＤが容易に入手でき、小
型・安価であるため、各被測定物毎に取付可能である。
被測定物から検出器迄は、光ファイバーを使用して可動
部分なしに測定可能であるが、上述の方式では各点の蛍
光が同時に検出器に入射するのて、各点を個別に測定す
るには光スイッチを設けて順次測定点を切換えなくては
ならないため、必ず光の損失を伴う欠点がある。一般的
には、光学測定系は安定状態で測定するのが原則である
がＬＥＤについては点灯後の光量変化が比較的短時間に
安定することから、光源の点・滅で測定点側の切換えを
行うことが可能である。The most desirable type is to have a fluorescence measuring device for each object to be measured.
In general, a fluorescence measuring device is for measuring a small amount of light, which is expensive and requires a large volume, so that individual installation becomes impossible. Recently, a high-brightness LED is easily available as an excitation light source, and since it is small and inexpensive, it can be attached to each object to be measured.
From the object to be measured to the detector, it is possible to measure without using any moving parts by using an optical fiber. However, in the above method, the fluorescence of each point is incident on the detector at the same time, so it is necessary to measure each point individually. Since the optical switch has to be provided with an optical switch to sequentially switch the measuring points, there is always a drawback that light loss occurs. Generally, the optical measurement system is measured in a stable state in principle, but for LEDs, the change in the light amount after lighting is stable in a relatively short time, so switching the measurement point side by turning on / off the light source. It is possible to
【０００５】[0005]
【作用】本発明の一例を図によって説明する。１は試料
容器で蛍光物質を含む試料が採取される。２は励起光光
源で、ここでは緑色ＬＥＤが使用される。蛍光検出は、
一般には発光が微弱であるため強力な励起光をあてるべ
く、ハロゲンランプから干渉フィルタ回析格子などの分
光手段により最適な励起光などの光が分光されるが、ハ
ロゲンランプは点灯初期に光量が不安定で光量が落着く
まで数分間を必要とするので本発明には使用できない。
これに反してＬＥＤでは数msec〜数10msecで光量が安定
するので、本発明のような使い方が可能である。An example of the present invention will be described with reference to the drawings. A sample container 1 collects a sample containing a fluorescent substance. 2 is an excitation light source, and here a green LED is used. Fluorescence detection
In general, since the light emission is weak, the light such as the optimum excitation light is dispersed from the halogen lamp by the spectroscopic means such as the interference filter diffraction grating in order to apply the strong excitation light. It cannot be used in the present invention because it is unstable and it takes several minutes for the light amount to settle down.
On the contrary, in the case of the LED, the light quantity is stabilized in several msec to several tens msec, and therefore, the usage like the present invention is possible.
【０００７】試料容器巾の試料の発生する蛍光を光ファ
イバー３に導く方法は常法による。レンズ系を介しても
よく、試料容器が充分大きいときは、直接光ファイバー
で受光してもよい。蛍光を導く光ファイバーを１ケ所に
集めて光電子増倍管４で測定を行うが、この部分には光
学系の工夫が必要となる。低暗電流の光電子増倍管ほど
受光面積が小さく多数本の光ファイバーからの拡散光を
受光面内に入射させるには、使用する光ファイバーの径
にもよるが、本数に制限を生じ、場合によっては複数本
の光電子増倍管が必要となる。この部分の光学系につい
ては光電子増倍管の受光面積、光ファイバーの径、本
数、ニューメリカルアパーチャーなどパラメーターが多
く、本発明を規定するものではない。A method for guiding the fluorescence generated by the sample having the width of the sample container to the optical fiber 3 is a conventional method. A lens system may be used, and when the sample container is sufficiently large, light may be directly received by an optical fiber. The optical fibers for guiding the fluorescence are collected at one place and the measurement is carried out by the photomultiplier tube 4, but this part requires a devised optical system. A photomultiplier tube with a low dark current has a smaller light receiving area, and in order to make diffused light from a large number of optical fibers enter the light receiving surface, the number of optical fibers used is limited, depending on the diameter of the optical fibers used. Multiple photomultiplier tubes are required. The optical system of this portion has many parameters such as the light receiving area of the photomultiplier tube, the diameter of the optical fiber, the number of the optical fibers, and the numerical aperture, and does not define the present invention.
【０００８】光電子増倍管の出力は常法によりホトンカ
ウンティングユニット又はアナログ増巾回路などの電子
回路５により処理される。これらの励起光用ＬＥＤは定
電流回路８を含むスイッチィング回路６により点灯を制
御され、順次・測定に必要な時間だけ点灯されると共
に、個々のＬＥＤの発光特性が同じになるように動作電
流が制御される。すなわち、ＬＥＤは発光特性にばらつ
きがあり、同じ電流を流しても同一の発光をしないの
で、ほぼ同じ光量が得られるように電流を制御する必要
がある。ここで制御されないばらつきは、データ処理部
５で補正してもよい。The output of the photomultiplier tube is processed in a conventional manner by an electronic circuit 5 such as a photon counting unit or an analog amplification circuit. Lighting of these LEDs for excitation light is controlled by a switching circuit 6 including a constant current circuit 8, and the LEDs are sequentially lit for a time required for measurement, and the operating current is adjusted so that the individual LEDs have the same emission characteristics. Is controlled. That is, since the LEDs have different light emission characteristics and do not emit the same light even if the same current is applied, it is necessary to control the current so that almost the same amount of light is obtained. Variations not controlled here may be corrected by the data processing unit 5.
【０００９】[0009]
【実施例】ローダミン標識抗体を結合させた検体を含む
６mm×６mmの試料容器１０ケの各々に緑ＬＥＤを近接し
て設置し、各器底より、コア径0.2mm 、クラッド径0.23
mmのプラスチックス光ファイバーで光電子増倍管に導い
た。１０本の光ファイバーの先端は微小レンズを設けて
光電子増倍管の受光面に結像させた。ＬＥＤの発光量が
ほぼ等しくなるように調整した電源回路を順次マルチプ
レクサーにより切替えて、各１秒づつ、ＬＥＤを点灯さ
せ、光電子増倍管の出力を測定したところ、試料濃度に
比例した出力が得られた。[Example] A green LED was placed close to each of 10 6 mm x 6 mm sample containers containing a sample to which a rhodamine-labeled antibody was bound, and a core diameter of 0.2 mm and a clad diameter of 0.23 were provided from the bottoms of the respective vessels.
It was led to a photomultiplier tube with mm plastics optical fiber. A microlens was provided at the tip of the ten optical fibers to form an image on the light receiving surface of the photomultiplier tube. When the output of the photomultiplier tube was measured by switching the power supply circuits adjusted so that the light emission amounts of the LEDs were almost equal by the multiplexer sequentially, turning on the LEDs for 1 second each, and measuring the output of the photomultiplier tube. Was obtained.
【００１０】[0010]
【発明の効果】本発明は、蛍光発光の場より光ファイバ
ーを用いて少数の光検出素子に導き、各点の励起光光源
を順番に測定に必要時間点灯させることにより測定点を
順次切換えて、可動部分なしに各点の蛍光を測定するも
ので、血中の薬物濃度や免疫グロブリンの濃度などの測
定を行う蛍光検出法で高感度検出と対象検体数の多数を
高能率に測定可能とした。Industrial Applicability The present invention guides a small number of photo-detecting elements using an optical fiber from the field of fluorescence emission, and sequentially switches the measurement points by sequentially turning on the excitation light source at each point for a necessary time for measurement, It measures fluorescence at each point without any moving parts.High-sensitivity detection and high-efficiency measurement of a large number of target samples can be performed with a fluorescence detection method that measures the concentration of drugs and immunoglobulin in blood. .
【図１】本発明の一実例を示す蛍光検出法の説明図であ
る。FIG. 1 is an explanatory diagram of a fluorescence detection method showing an example of the present invention.
１ 試料容器 ２ 励起光光源 ３ 光ファイバー ４ 光電子増倍管 ５ 電子回路 ６ スイッチング回路 ７ データ処理部 ８ 定電流電源 ９ 光学系 1 sample container 2 excitation light source 3 optical fiber 4 photomultiplier tube 5 electronic circuit 6 switching circuit 7 data processing unit 8 constant current power supply 9 optical system
─────────────────────────────────────────────────────
─────────────────────────────────────────────────── ───
【手続補正書】[Procedure amendment]
【提出日】平成５年１２月１０日[Submission date] December 10, 1993
【手続補正１】[Procedure Amendment 1]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】全文[Correction target item name] Full text
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【書類名】 明細書[Document name] Statement
【発明の名称】 蛍光検出法Title of invention Fluorescence detection method
【特許請求の範囲】[Claims]
【発明の詳細な説明】Detailed Description of the Invention
【０００１】[0001]
【産業上の利用分野】本発明は多数の蛍光性サンプルを
１台の測定器で測定する場合に有用な発明であり、励起
光の照射により蛍光性サンプルから発光する蛍光を検出
し、血中の薬物濃度や免疫グロブリン濃度や医療分野で
官能基特異性等の測定を行う蛍光検出法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention is an invention useful when a large number of fluorescent samples are measured by a single measuring instrument. The present invention relates to a fluorescence detection method for measuring drug concentration, immunoglobulin concentration, functional group specificity, etc. in the medical field.
【０００２】[0002]
【従来の技術】蛍光測定法としては特開昭６３−２０８
７３３号に開示したものがあるが、殆んどの方式はこれ
らを応用した蛍光測定装置へ被測定物を運搬して測定し
測定終了後に排出する方式であり、試験片の運搬装置を
必要とする。この他、蛍光測定装置を被測定物のライン
に移動する方法もあるが、いずれも、上述の方法は機械
的な動作を伴うので、磨耗、焼付などに起因する故障が
多く好ましくない。2. Description of the Related Art A fluorescence measuring method is disclosed in JP-A-63-208.
Although there is a method disclosed in Japanese Patent No. 733, most of the methods are methods for transporting and measuring an object to be measured to a fluorescence measuring device to which these are applied, and discharging after the completion of the measurement, which requires a test piece transporting device. . In addition to this, there is also a method of moving the fluorescence measuring device to the line of the object to be measured, but both of them are not preferable because many of the above methods involve mechanical operations, and therefore there are many failures due to wear and seizure.
【０００３】[0003]
【発明が解決しようとする課題】上述したように従来技
術は、蛍光測定装置へ被測定物を運搬及び排出する運搬
装置や、蛍光測定装置を被測定物に移動する装置を持た
ねばならないので、これらの移送装置を除去することに
より測定の信頼性の向上を図ることを課題とした。As described above, the prior art must have a transporting device for transporting and discharging an object to be measured to the fluorescence measuring device and a device for moving the fluorescence measuring device to the object to be measured. The object was to improve the reliability of measurement by removing these transfer devices.
【０００４】[0004]
【課題を解決するための手段】最も望ましい型式は各被
測定物毎に蛍光測定装置を持つことであるが、これは、
一般的に蛍光測定装置が微量光測定であり、高価、大容
積を要することから個別設置は無理を生ずる。近来、励
起光光源としては高輝度の光源が容易に入手でき、小型
・安価であるため、各被測定物毎に取付可能である。被
測定物から検出器迄は、光ファイバーを使用して可動部
分なしに測定可能であるが、上述の方式では各点の蛍光
が同時に検出器に入射するので、各点を個別に測定する
には光スイッチを設けて順次測定点を切換えなくてはな
らないため、必ず光の損失を伴う欠点がある。一般的に
は、光学測定系は安定状態で測定するのが原則であるが
ＬＥＤについては点灯後の光量変化が比較的短時間に安
定することから、光源の点・滅で測定点側の切換えを行
うことが可能である。The most desirable type is to have a fluorescence measuring device for each object to be measured.
In general, a fluorescence measuring device is for measuring a small amount of light, which is expensive and requires a large volume, so that individual installation becomes impossible. Recently, a high-intensity light source is easily available as an excitation light source, and since it is small and inexpensive, it can be attached to each object to be measured. It is possible to measure from the object to be measured to the detector without using any moving parts, but in the above method, the fluorescence of each point is incident on the detector at the same time, so it is necessary to measure each point individually. Since an optical switch must be provided to sequentially switch the measurement points, there is a drawback that light loss is always involved. Generally, the optical measurement system is measured in a stable state in principle, but for LEDs, the change in the light amount after lighting is stable in a relatively short time, so switching the measurement point side by turning on / off the light source. It is possible to
【０００５】[0005]
【作用】本発明の一例を図によって説明する。１は試料
容器で多くの場合は反応により蛍光物質が生成される。
２は励起光光源で、ここでは緑色ＬＥＤ又は半導体レー
ザー等が使用される。蛍光検出は、一般には発光が微弱
であるため強力な励起光をあてるべく、ハロゲンランプ
から干渉フィルタ、回析格子などの分光手段により最適
な励起波長の光が分光されるが、ハロゲンランプは点灯
初期に光量が不安定で光量が落着くまで数分間を必要と
するので本発明には使用できない。これに反してＬＥＤ
では数ｍｓｅｃ〜数１０ｍｓｅｃで光量が安定するの
で、本発明のような使い方が可能である。An example of the present invention will be described with reference to the drawings. Reference numeral 1 denotes a sample container, and in many cases, a fluorescent substance is produced by the reaction.
Reference numeral 2 is an excitation light source, and a green LED, a semiconductor laser or the like is used here. In fluorescence detection, light emission is generally weak, so in order to irradiate strong excitation light, the light of the optimum excitation wavelength is separated from the halogen lamp by a spectroscopic means such as an interference filter or a diffraction grating, but the halogen lamp is turned on. Since the light intensity is unstable in the initial stage and it takes several minutes for the light intensity to settle down, it cannot be used in the present invention. On the contrary, LED
Since the amount of light is stable in several msec to several tens of msec, it can be used as in the present invention.
【０００６】試料容器巾の試料の発生する蛍光を光ファ
イバー３に導く方法は常法による。レンズ系を介しても
よく、試料容器が充分大きいときは、直接光ファイバー
で受光してもよい。蛍光を導く光ファイバーを１ケ所に
集めて光電子増倍管４で測定を行うが、この部分には光
学系の工夫が必要となる。低暗電流の光電子増倍管ほど
受光面積が小さく多数本の光ファイバーからの拡散光を
受光面内に入射させるには、使用する光ファイバーの径
にもよるが、本数に制限を生じ、場合によっては複数本
の光電子増倍管が必要となる。この部分の光学系につい
ては光電子増倍管の受光面積、光ファイバーの径、本
数、ニューメリカルアパーチャーなど、パラメーターが
多く、本発明を規定するものではない。A method for guiding the fluorescence generated by the sample having the width of the sample container to the optical fiber 3 is a conventional method. A lens system may be used, and when the sample container is sufficiently large, light may be directly received by an optical fiber. The optical fibers for guiding the fluorescence are collected at one place and the measurement is carried out by the photomultiplier tube 4, but this part requires a devised optical system. A photomultiplier tube with a low dark current has a smaller light receiving area, and in order to make diffused light from a large number of optical fibers enter the light receiving surface, the number of optical fibers used is limited, depending on the diameter of the optical fibers used. Multiple photomultiplier tubes are required. The optical system of this portion has many parameters such as the light receiving area of the photomultiplier tube, the diameter of the optical fiber, the number of the optical fibers, and the numerical aperture, and does not define the present invention.
【０００７】光電子増倍管の出力は常法によりホトンカ
ウンティングユニット又はアナログ増巾回路などの電子
回路５により処理される。これらの励起光用ＬＥＤは定
電流回路８を含むスイッチィング回路６により点灯を制
御され、順次・測定に必要な時間だけ点灯されると共
に、個々のＬＥＤの発光特性が同じになるように動作電
流が制御される。すなわち、ＬＥＤは発光特性にばらつ
きがあり、同じ電流を流しても同一の発光をしないの
で、ほぼ同じ光量が得られるように電流を制御する必要
がある。ここで制御されないばらつきは、データ処理部
５で補正してもよい。The output of the photomultiplier tube is processed by an electronic circuit 5 such as a photon counting unit or an analog amplification circuit in a conventional manner. Lighting of these LEDs for excitation light is controlled by a switching circuit 6 including a constant current circuit 8, and the LEDs are sequentially lit for a time required for measurement, and the operating current is adjusted so that the individual LEDs have the same emission characteristics. Is controlled. That is, since the LEDs have different light emission characteristics and do not emit the same light even if the same current is applied, it is necessary to control the current so that almost the same amount of light is obtained. Variations not controlled here may be corrected by the data processing unit 5.
【０００８】[0008]
【実施例】ローダミン標識抗体を結合させた検体を含む
６ｍｍ×６ｍｍの試料容器１０ケの各々に緑ＬＥＤを近
接して設置し、各容器底より、コア径０．２ｍｍ、クラ
ッド径０．２３ｍｍのプラスチックス光ファイバーで光
電子増倍管に導いた。１０本の光ファイバーの先端は微
小レンズを設けて光電子増倍管の受光面に結像させた。
ＬＥＤの発光量がほぼ等しくなるように調整した電源回
路を順次マルチプレクサーにより切替えて、各１秒づ
つ、ＬＥＤを点灯させ、光電子増倍管の出力を測定した
ところ、試料濃度に比例した出力が得られた。[Example] A green LED was installed in proximity to each of 10 6 mm x 6 mm sample containers containing a sample to which a rhodamine-labeled antibody was bound, and a core diameter of 0.2 mm and a clad diameter of 0.23 mm from the bottom of each container. Led to a photomultiplier tube with a plastic optical fiber. A microlens was provided at the tip of the ten optical fibers to form an image on the light receiving surface of the photomultiplier tube.
When the output of the photomultiplier tube was measured by switching the power supply circuits adjusted so that the light emission amounts of the LEDs were almost equal by the multiplexer sequentially, turning on the LEDs for 1 second each, and measuring the output of the photomultiplier tube. Was obtained.
【０００９】[0009]
【発明の効果】本発明は、多数の蛍光発光の場より光フ
ァイバーを用いて少数の光検出素子に導き、各点の励起
光光源を順番に測定に必要時間点灯させることにより測
定点を順次切換えて、可動部分なしに各点の蛍光を測定
するもので、血中の薬物濃度や免疫グロブリンの濃度な
どの測定を行う蛍光検出法として好適である。Industrial Applicability According to the present invention, the measurement points are sequentially switched by guiding from a large number of fluorescence emission fields to a small number of photodetectors using optical fibers, and sequentially turning on the excitation light sources at each point for the required time for measurement. Thus, the fluorescence at each point is measured without moving parts, which is suitable as a fluorescence detection method for measuring the concentration of drug in the blood or the concentration of immunoglobulin.
【図面の簡単な説明】[Brief description of drawings]
【図１】本発明の一実例を示す蛍光検出法の説明図であ
る。FIG. 1 is an explanatory diagram of a fluorescence detection method showing an example of the present invention.
【符号の説明】 １ 試料容器 ２ 励起光光源 ３ 光ファイバー ４ 光電子増倍管 ５ 電子回路 ６ スイッチング回路 ７ データ処理部 ８ 定電流電源 ９ 光学系[Explanation of reference symbols] 1 sample container 2 excitation light source 3 optical fiber 4 photomultiplier tube 5 electronic circuit 6 switching circuit 7 data processing unit 8 constant current power supply 9 optical system
【手続補正３】[Procedure 3]
【補正対象書類名】図面[Document name to be corrected] Drawing
【補正対象項目名】図１[Name of item to be corrected] Figure 1
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【図１】 [Figure 1]
Claims (1)
の場より光ファイバーを用いて少数の光検出素子に導
き、各点の励起光光源を順番に測定に必要な時間点灯さ
せることにより測定点を順次切換えることにより、可動
部分なしに多点の蛍光を測定することを特徴とする蛍光
検出法。1. When measuring fluorescence at multiple points, by guiding from a field of fluorescence emission to a small number of photodetectors using an optical fiber, the excitation light sources at each point are sequentially turned on for a time required for measurement. A fluorescence detection method characterized by measuring fluorescence at multiple points without moving parts by sequentially switching measurement points.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27883693A JPH07120393A (en) | 1993-10-13 | 1993-10-13 | Fluorescence detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27883693A JPH07120393A (en) | 1993-10-13 | 1993-10-13 | Fluorescence detection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH07120393A true JPH07120393A (en) | 1995-05-12 |
Family
ID=17602834
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP27883693A Pending JPH07120393A (en) | 1993-10-13 | 1993-10-13 | Fluorescence detection method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07120393A (en) |
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- 1993-10-13 JP JP27883693A patent/JPH07120393A/en active Pending
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