JPH06125982A - Blood separation method - Google Patents

Abstract

(57) [Summary] [Structure] In addition to the blood sampling needle 3 and the blood sampling tube 2, whole blood is collected in the parent bag 1 having two or three connecting portions 12 and 13 at the lower portion, and the whole blood is collected. A blood separation method in which an upper layer containing plasma, a buffy coat 16 and a lower layer containing erythrocytes are centrifuged, and then the lower layer part is first transferred to each child bag 4 and 5 via each connection part and then the buffy coat 16 is transferred. . [Effect] In blood separation, a red blood cell concentrate containing no white blood cells can be obtained.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a blood separation method for obtaining a blood component preparation.

[0002]

2. Description of the Related Art A blood bag set is used to extract various blood components contained in whole blood to obtain a blood product.

FIG. 2 is a plan view showing a conventionally used blood bag set.

A conventional blood separation method using a blood bag set is as follows.

In addition to the blood collecting needle 3 and the blood collecting tube 2 being provided in the parent bag 1 at the connecting portion 9, whole blood is collected in the parent bag 1 provided with two or three connecting portions on the upper portion of the main body 8. Then, after centrifuging the whole blood into an upper layer containing plasma, an intermediate layer containing white blood cells (hereinafter referred to as buffy coat) and a lower layer containing red blood cells, the communication piece 14 is brought into a communication state, and the main body 8 is While pressing, the upper layer portion, that is, plasma is transferred from the connecting portion 12 to the child bag 4 through the tube 6.

Next, the communication piece 15 is brought into a communication state, and the buffy coat is transferred from the connection portion 13 to the child bag 5 through the tube 7 while pressing the main body 8.

As a result, immediately after centrifugation, a child bag containing the plasma portion in the upper layer and a child bag containing the buffy coat are obtained.

At this time, the concentrated red blood cells (hereinafter, referred to as CRC) in the lower layer immediately after centrifugation in the parent bag 1 were performed.
Remains. It is freely chosen to transfer it again to the child bag via the connection. In a recent example, a red blood cell preservation solution is added to CRC. As a method of addition relating to this, a small child bag (not shown) was separately prepared and the erythrocyte preservation solution was added to the CRC through the tube 7.

The thus obtained product is used as a blood product. At that time, blood is transfused to the patient from the port 11 with the protector 10 provided in the bag.

[0010]

The conventional blood separation method as described above has the following problems.

(1) Even after the buffy coat is transferred, a part of the inner surface of the main body 8 is stained with the buffy coat. (2) As described above, when adding the red blood cell preservative solution to the CRC, the red blood cell preservative solution containing the buffy coat adhering and remaining in the tube 7 was added to the CRC.

When the CRC is obtained in the conditions (1) and (2), only an impure CRC containing a small amount of buffy coat can be obtained. Naturally, such impure CRC contains white blood cells that were in the buffy coat.

Transfusion of such impure CRC into the human body causes the following serious problems as a side effect.

(I) Non-hemolytic transfusion side effects (fever / post-transfusion pulmonary symptoms) occur with the production of alloantibodies (anti-HLA). (Ii) Graft-versus-host disease (GVHD) is caused by lymphocytes in leukocytes, especially T cells, and in most cases, death occurs. (Iii) White blood cells serve as a transferer for viruses such as cytomegalovirus (CMV) and HTLV-I, resulting in transfusion virus infection.

The present invention was invented to obtain a CRC containing no buffy coat in order to obviate the serious problems of (i), (ii) and (iii).

[0016]

According to the present invention, whole blood is collected in a parent bag which is provided with two or three connecting portions in the lower part in addition to a blood collecting needle and a blood collecting tube, and this whole blood is converted into plasma. It is characterized in that after centrifuging into an upper layer containing it, an intermediate layer containing white blood cells and a lower layer containing red blood cells, the lower layer part is first transferred to each child bag via each connection part, and then the intermediate layer part is transferred. The main point is the blood separation method.

[0017]

When the transfer operation of the buffy coat is performed, the inner wall of the parent bag and the inside of the tube connecting the bags are contaminated with the buffy coat. However, since the blood separation method of the present invention transfers the CRC before the transfer of the buffy coat, that is, Since the CRC is transferred before being polluted by the buffy coat in the inner wall of the parent bag or the tube connecting the bags, a CRC that is not polluted by the buffy coat can be obtained.

That is, in the conventional blood separation method, the blood plasma portion was first separated into the baby bags in the order of the buffy coat, whereas the blood separation method of the present invention was firstly CRC and then the buffy coat. CRC will not be contaminated by buffy coat because it will be separated into child bugs in order of
Is obtained.

[0019]

FIG. 1 is a plan view showing a blood bag set used in an embodiment of the present invention. The blood separation method of the present invention will be described with reference to FIG. 1 as follows.

In addition to the blood collecting needle 3 and the blood collecting tube 2 being provided in the parent bag 1 at the connecting portion 9, whole blood is collected in the parent bag 1 provided with two connecting portions 12 and 13 at the bottom of the main body 8. Then, the parent bag 1 is installed in the lower portion of the main body 8 so that gravity is applied more, and centrifugation is performed. The conditions for centrifugation are usually 3000 to 4500 xg, 6 to 10 minutes, preferably 44.
00 × g, 6 minutes.

By the above centrifugation, whole blood is separated into an upper layer (not shown) containing plasma, a buffy coat 16 and a lower layer containing red blood cells (not shown).
With the communication piece 14 in the communication state, the lower layer portion, that is, the CRC is transferred from the connection portion 12 to the child bag 4 via the tube 6 while pressing the main body 8. It is preferable that a red blood cell preservative solution is previously put in the child bag 4.

Next, the communication piece 15 is set in the communication state, and the buffy coat 16 is transferred from the connection portion 13 to the child bag 5 via the tube 7 while pressing the main body 8.

Thus, the child bag 4 containing the CRC and the child bag 5 containing the buffer coat 16 are obtained. Further, in order to separate the platelets from the buffy coat 16, only the child bag 5 is centrifuged, and the upper layer is transferred to another child bag (not shown). The conditions of centrifugation at this time are mild, and 180 × g and 6.5 minutes are preferable.

When the blood component is transferred to the child bag as described above, the plasma portion existing in the upper layer remains in the parent bag 1 immediately after centrifugation.

The blood product is obtained in this manner, and when used, blood is transfused into the human body through the port 11 with the protector 10 provided in the bag.

[0026]

Since the blood separating method of the present invention uses almost the same blood bag set as in the conventional method, almost all the steps of manufacturing the blood bag set can be followed, and the operation of obtaining a blood product can be carried out in almost the same manner as the conventional method.

However, in the method of the present invention, there is no concern that the CRC contains white blood cells.

In the case of adding the erythrocyte preservation solution, conventionally, a child bag was separately prepared and added to the CRC through a tube whose inner wall was soiled with a buffer coat, but in the method of the present invention, the CRC is stored. It is only necessary to add the red blood cell preservative solution to the baby bag in advance, and thus the operation of adding the red blood cell preservative solution becomes unnecessary.

[Brief description of drawings]

FIG. 1 is a plan view showing a blood bag set used in an embodiment of the present invention.

FIG. 2 is a plan view showing a blood bag set which has been conventionally used.

[Explanation of symbols]

 1 parent bug 4 child bug 5 child bug 16 buffy coat

Claims (1)

[Claims] 1. A whole blood is collected in a parent bag having two or three connecting portions at a lower portion in addition to a blood collecting needle / blood collecting tube, and this whole blood contains an upper layer containing plasma and white blood cells. A method for blood separation, which comprises centrifuging an intermediate layer and a lower layer containing red blood cells, and then transferring the lower layer part and then the intermediate layer part to each child bag via each connection part.