JPH06105904A - Blood separation method and blood bag set - Google Patents

Abstract

(57) [Summary] [Construction] An upper connecting part 12 is provided on the upper left end of the parent bag 1, and a lower connecting part 13 is provided on the lower left end of the parent bag 1. Part 12, 1
The right end of 3 is open at the top and bottom inside the parent bag 1. After the whole blood sample in the parent bag 1 is centrifuged into the upper layer 17, the middle layer 18, and the lower layer 19, the parent bag 1
Are squeezed, the upper layer 17 and the lower layer 1 inside the parent bag 1
9 is an upper / lower child bag 4 through upper / lower connecting portions 12 and 13 and upper / lower tubes 6 and 7.
Transferred to 5 at the same time. [Effect] In addition to being advantageous in terms of labor saving and shortening of working time, when the blood bag set is put on the centrifugal separator, there is less possibility that a protrusion is formed on the parent bag 1, and therefore the parent bag 1 Is unlikely to be damaged by the above-mentioned protrusion.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood separation method and a blood bag set used in this method.

[0002]

2. Description of the Related Art A blood bag set is used for taking out various blood components contained in a whole blood sample and formulating them. As a blood separation method using a blood bag set, there is one shown in Japanese Patent Publication No. 63-20144, in which an upper connection part is provided at the upper end of a parent bag from which a whole blood sample is collected, The lower part of the parent bag is provided with a lower connecting part, and the upper connecting part and the lower connecting part are connected to the upper connecting part and the lower connecting part via the upper tube and the lower connecting tube, respectively. Has been done.

When separating the whole blood sample collected in the parent bag into blood components, the whole blood bag set is placed in a centrifuge and the whole blood sample in the parent bag is centrifuged. As a result, an upper layer containing plasma is present in the upper part of the parent bag, a thin intermediate layer (buffy coat) containing white blood cells is provided in the middle part, and a lower layer containing red blood cells is provided in the lower part. , Separated and formed. Then, the parent bag is squeezed by the blood separation squeezing device, each blood component in the upper and lower parts of the inside of the parent bag, via each connection and each tube, to the upper child bag and the lower child bag, Simultaneously transferred.

[0004] The above blood separation method is very advantageous in terms of labor saving and shortening of working time because the blood components in the upper and lower parts inside the parent bag can be transferred to the two child bags at the same time. By the way, when centrifuging a blood bag set with a centrifuge, the blood bag set is set in the centrifuge so that the tubes do not come into contact with the bags.

[0005]

However, in the above-mentioned prior art, the upper connecting portion is provided at the upper end of the parent bag, and the lower connecting portion is provided at the lower end of the parent bag. Since the lower part and the lower connecting part are separately provided, the upper tube or the lower tube inevitably comes into contact with the front surface or the rear surface of the parent bag when the blood bag set is set in the centrifuge. Easy to set in the state.

[0006] When set in this way, when centrifuging, the front or rear surface of the parent bag is strongly pressed against the upper tube or the lower tube, and the periphery of the pressed portion of the parent bag swells, Protrusions are easily formed, and in particular, the lower portion of the parent bag is subject to a large gravitational acceleration during centrifugation, so that the protrusions are easily formed. Then, as described above, when the protrusion is formed, the parent bag is easily damaged from the protrusion, and there has been a problem how to solve this problem. The present invention solves the above problems, and is advantageous in terms of labor saving and working time reduction, and a blood separation method and a blood bag set that are less likely to damage the parent bag by the protrusion. The purpose is to provide.

[0007]

In order to solve the above-mentioned problems, the blood separation method of the present invention is characterized in that an upper connecting portion is provided at an upper portion of a side portion and a lower connecting portion is provided at a lower portion of the side portion. A whole blood sample was collected in a parent bag provided with a connection part, and this whole blood sample was centrifuged into an upper layer containing plasma, an intermediate layer containing white blood cells, and a lower layer containing red blood cells. The upper layer is connected to the upper child bag through the upper connecting portion opening at the upper portion inside the parent bag,
In addition, the lower layer is simultaneously transferred to the lower child bag through the lower connecting portion that opens at the lower portion inside the parent bag. Further, the feature of the blood bag set of the present invention is that a parent bag from which a whole blood sample is collected, an upper child bag and a lower child bag are respectively connected via an upper connecting portion and a lower connecting portion. The whole blood sample inside the parent bag is centrifuged into an upper layer containing plasma, an intermediate layer containing white blood cells, and a lower layer containing red blood cells,
In the one in which the upper layer is transferred to the upper child bag through the upper connecting portion, and the lower layer is transferred to the lower child bag through the lower connecting portion, respectively, the side of the parent bag The upper part has a connecting part for the upper part that opens at the upper part inside the parent bag, and the lower part of the side part of the parent bag has a connecting part for the lower part that opens at the lower part inside the parent bag. .

[0008]

[Function] When collecting a whole blood sample and centrifuging, first, after introducing the whole blood sample into the parent bag,
Place the blood bag set in the centrifuge. On this occasion,
Since the upper part and the lower part are collectively provided on the side of the parent bag, when the blood bag set is set in the centrifuge, the upper tube and the lower tube are attached to the front surface of the parent bag. Or, it is less likely to be set in contact with the rear surface.

When the whole blood sample in the parent bag is centrifuged, an upper layer containing plasma is present in the upper portion of the parent bag and a thin intermediate layer (buffy coat) containing white blood cells is present in the middle portion. A lower layer containing red blood cells is separately formed in the lower portions. After that, the parent bag is squeezed, and the blood components in the upper and lower portions inside the parent bag are simultaneously transferred to the upper and lower child bags via the upper and lower connecting portions.

[0010]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings. The drawing shows a blood bag set, with a parent bag 1,
It has a blood collection needle 3 with a blood collection tube 2, upper / lower child bags 4, 5 and upper / lower tubes 6, 7 and the like. The parent bag 1 is used to collect a whole blood sample, and is mainly composed of a vertically long rectangular main body 8. The main body 8 is composed of two sheets of synthetic resin such as polyvinyl chloride. , The peripheries of these sheets are adhered. A connection part 9 to which the blood collection tube 2 is connected is provided at the center of the left end of the body 8 in the up-down direction, and an upper connection part 12 is provided at the upper part of the left end of the body 8. A lower connecting portion 13 is provided below the left end of the main body 8.

In the embodiment, the upper and lower connecting portions 12 and 13 are made of, for example, a soft tube made of a synthetic resin such as polyvinyl chloride, and are used to bond the two sheets of the main body 8 together. , It is sandwiched between both seats and fixed. The left side portions of the upper and lower connecting portions 12 and 13 project to the left of the main body 8, and the left side portions are provided with communication pieces 14 and 15 that allow the inside and the outside of the main body 8 to communicate with each other. Has been. The right end of the upper connecting portion 12 opens at the upper portion inside the main body 8, and the right end of the lower connecting portion 13 opens at the lower portion inside the main body 8.
The right end openings 2 and 13 are located at the same position as the right end inside the main body 8 in the left-right direction.

The parent bag 1 is provided with the respective connecting portions 9, 12, 13 at the left end instead of the upper and lower ends as in the conventional case, and it is possible to determine which side of the parent bag 1 is the upper side. Hard to do. Therefore, as shown by the phantom line in FIG.
A horizontally long label L on which the name of the manufacturing company is written is attached to the central portion in the vertical direction of so that the upper side of the parent bag 1 can be identified. This can prevent mistakes when the parent bag 1 is set in the blood separation device. In addition, inside the main body 8,
A blood preservation solution such as a CPD solution or an ACD-A solution is enclosed.

The upper and lower child bags 4 and 5 are respectively
Each blood component in the upper part and the lower part inside the main body 8 is transferred, and is connected to the upper and lower connecting parts 12 and 13 of the main body 8.
They are connected via upper and lower tubes 6 and 7. According to the embodiment configured as described above, when collecting and centrifuging a whole blood sample, first, the whole blood is collected from the donor through the blood collecting needle 3, the blood collecting tube 2, and the connecting portion 9. The sample is introduced into the main body 8 of the parent bag 1. Then, the blood bag set is put in the centrifuge, and the blood in the main body 8 is centrifuged at 4540 × g for 6 minutes,
At the upper part inside the main body 8, an upper layer 17 containing plasma is
A thin intermediate layer (buffy coat) 18 containing white blood cells and platelets is formed in the intermediate portion, and a lower layer 19 containing red blood cells is separately formed in the lower portion.

At this time, since the connecting portion 9 for connecting the blood collecting tube 2, the connecting portion 12 for the upper portion and the connecting portion 13 for the lower portion are collectively provided on the right end portion of the parent bag 1, the blood bag set is set. When set in the centrifuge, the blood collection tube 2, the upper tube 6, and the lower tube 7 are less likely to be set in contact with the front surface or the rear surface of the main body 8 of the parent bag 1. Therefore, as in the conventional case, the main body 8 of the parent bag 1 is replaced by the blood collection tube 2,
By being strongly pressed against the upper tube 6 or the lower tube 7, the periphery of the pressed portion of the main body 8 swells,
The problem that the protrusion is formed and the main body 8 is easily damaged from the protrusion is unlikely to occur.

Then, after centrifuging the whole blood sample as described above, the end of the blood collection tube 2 on the side of the connecting portion 9 is shut off, and then the communicating pieces 14 and 15 are bent to form the main body 8 of the parent bag 1. The upper and lower inner parts and the upper and lower child bags 4, 5 are made to communicate with each other via the upper and lower connecting portions 12, 13 and the upper and lower tubes 6, 7. next,
The main body 8 of the parent bag 1 is squeezed by, for example, a blood separation squeezing device so that the blood components in the upper layer 17 and the lower layer 19 inside the main body 8 are connected to the upper / lower connecting portions 12, 13 and the upper portion. It is simultaneously transferred to the upper and lower child bags 4 and 5 through the upper and lower tubes 6 and 7.

In this way, the blood components in the upper and lower parts of the main body 8 can be easily transferred to the two sub-bags 4 and 5 at the same time, which is very advantageous in terms of labor saving and shortening of working time. is there. In the example, the centrifugation was performed with a large centrifugal force of 4540 × g so that the intermediate layer contained platelets. However, the centrifugal separation force was reduced so that the upper layer contained platelets. Sometimes Further, in the embodiment, the connecting portions for upper and lower portions are made of tubes, but these connecting portions may be formed integrally with the main body of the parent bag. You may integrate with the tube for each. Further, in the embodiment, the upper connection portion and the lower connection portion are addressed to one, respectively,
Multiple addresses may be sent.

[0017]

As described in detail above, according to the present invention,
In the work of extracting blood components from a whole blood sample, it is very advantageous in terms of labor saving and shortening of working time, and when the blood bag set is subjected to a centrifuge, a protrusion is formed on the parent bag. Therefore, there is little fear that the parent bag will be damaged from the protrusion.

[Brief description of drawings]

FIG. 1 is a front view of a blood bag set showing an embodiment of the present invention.

[Explanation of symbols]

1 ... Parent bag, 4, 5 ... Upper / lower child bag, 6,
7 ... Upper / lower tubes, 8 ... Main body, 12, 13 ...
Upper / lower connection part, 17 ... upper layer, 18 ... intermediate layer,
19 ... Lower layer.

Claims (2)

[Claims] 1. A whole blood sample is collected in a parent bag in which an upper connecting portion is provided at an upper portion of a side portion and a lower connecting portion is provided at a lower portion of a side portion, and the whole blood sample is collected as plasma. Containing an upper layer, a white blood cell-containing intermediate layer, and a red blood cell-containing lower layer, and the upper layer is separated from the upper layer through an upper connection opening at the upper part inside the parent bag. A method for separating blood, characterized in that the lower layer is simultaneously transferred to a bag and to a lower child bag through a lower connecting portion that opens at a lower part inside a parent bag. 2. An upper bag and a lower bag are connected to a parent bag from which a whole blood sample is collected via an upper connecting portion and a lower connecting portion, respectively, and the whole blood inside the parent bag is connected. The sample is centrifuged into an upper layer containing plasma, an intermediate layer containing white blood cells, and a lower layer containing red blood cells, and the upper layer is connected via the upper connection to the upper child bag, Further, in the case where the lower layer is transferred to the lower child bag via the lower connecting portion, the upper connecting portion for opening at the upper portion inside the parent bag is provided on the upper side portion of the parent bag. At the same time, the blood bag set is characterized in that the lower side portion of the parent bag is provided with a lower connecting portion that opens at the lower portion inside the parent bag.