JPH0597647A - Cosmetic - Google Patents

Abstract

PURPOSE:To provide a cosmetic containing a safe ingredient having a high whitening action and giving a wet touch to skin when applied to the skin, and having high effects on the prevention of skin roughness and fine wrinkles. CONSTITUTION:A solvent extract is compounded in a dry amount of 0.00005-5wt.% preferably 0.005-2wt.%, as an essential ingredient, with a cosmetic such as lotion, cream, emulsion, or pack to obtain a cosmetic having a high whitening action, smoothening skins and holding the skins in the young states. The solvent extract is obtained by extracting the barks or leaves of a Machilus plant belonging to the Lauraceae, e.g. easily available Machilus thunbergii or Machilus japonica naturally growing in the country, with water or an organic solvent such as ethanol, methanol, acetone or ethyl ether, preferably ethanol. Since the whitening action is not exhibited dependent on conditions when the amount of the water is >=30wt.% in the extract, the amount of the water is necessary to be taken care, and the utilization of the extract as lyophilized powder is also effective in dependence on its utilization method.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic having a high whitening effect, smoothing the skin and keeping youthful skin.

[0002]

2. Description of the Related Art Plants of the genus Tabanus belong to the family of dicotyledonous plants, sessile subflora, and duckweed. Tabuki (Machi
lus thunbergii) Also known as Inugus
japonica) Another name, Aogashi, grows naturally. Tabuki is distributed in Honshu, Shikoku, Kyushu, Ryukyu and South Korea, China, etc.,
It is an evergreen tree that is often found along the warm coast. On the other hand, Hosobatab is an evergreen tree that is distributed west of Honshu, Ogasawara, Shikoku, Kyushu, Ryukyu and South Korea, and is found in the mountains of warm regions.

The bark of this tree is used in Korea as a substitute for Korean magnolia, which is also referred to as "thin earthworm", and is used as a medicine. However, Magnolia obovata from Magnolia family is used in Koboku, and Magnolia o
The bark of fficinalis is used. With respect to these, the present inventors have already invented the usage method for cosmetics in Japanese Patent Application No. 2-193030, Japanese Patent Application No. 2-193031 and Japanese Patent Application No. 2-193032. The present inventors have also found that those actions of Koboku are expressed by magnolol and honokiol.

The bark and leaves of the genus Tabanus have not been used as a raw material for cosmetics, and in JP-A-63-196514, they are only slightly used as pharmaceuticals for prophylactic / therapeutic agents for protozoal diseases.

[0005]

SUMMARY OF THE INVENTION An object of the present invention is to provide a whitening cosmetic composition which is safe when applied to the skin, has a large whitening effect, and contains a component that gives a moist feeling to the skin.

[0006]

[Means for Solving the Problems] The inventors of the present invention also experimented with Korean-produced koboku in the process of studying koboku, which is very effective as a raw material for cosmetics, and found that it similarly inhibited tyrosinase activity. It was Therefore, when we confirmed the magnolol and honokiol by liquid chromatography, it was found that most of both substances were not contained in Korean artefact, and it was found that another substance exerts its action,
And the active oxygen production inhibitory effect related to aging prevention is also not present in magnolol and honokiol, and also in magnolia, and it is found that it is present in extracts of Taboki and Hosobata, and it is highly effective. It confirmed and completed this invention. It was also found that the leaves of Taboki have the same effectiveness as a raw material for cosmetics, regardless of whether the components are the same or not.

The present invention is a cosmetic containing a solvent extract of bark and leaves of the genus Tabanuki of the family Asteraceae.

[0008] Taboki and Hosobata are native to Japan and are easily available. In addition, the powder of tabuki leaves and bark is used as a raw material for incense under the name of tab powder, so there is also a method of purchasing and using it.

An organic solvent such as water, ethanol,
Methanol, acetone, ethyl ether and the like are extracted alone or with a mixture of two or more solvents. The time, temperature and the like may be determined according to the type of solvent and the purpose. However, the ethanol extract is most suitable as a raw material for cosmetics.

However, when the proportion of water is 30% by weight or more, the whitening effect is not exerted depending on the conditions, so it is necessary to pay attention to the proportion of water. It is also effective to freeze-dry and use it as a powder depending on the method of use.

This substance can be used as other cosmetic raw materials such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin and 1,3 butylene glycol, and various drugs. It can be added to prepare various dosage forms of cosmetics. For example, a lotion, a cream, a milky lotion, a pack or the like may be used depending on the purpose. The effect of the extract of Tubus cypress extract varies depending on the dosage form to be compounded, the purpose of the product, or the presence or absence of a coexisting drug, but in terms of dry matter, 0.00005 to 5% by weight, preferably 0. 0.005 to 2% by weight is suitable.

[0012]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples. The production of the solvent extract of the genus Tabanus used in the examples above is described below.

(Production Example 1) Dry bark of bark tree 10
300 ml of methanol is added to g and the mixture is refluxed and cooled for 3 hours. This was filtered and freeze-dried. The yield was 0.92g.

(Production Example 2) Bark 10 of dried tabuki
300 g of 50% methanol aqueous solution is added to g and the mixture is refluxed and cooled for 3 hours. This was filtered and freeze-dried. Yield 1.1
It was 5 g.

(Production Example 3) Dry bark 10 of bark tree
300 ml of purified water was added to g and the mixture was refluxed and cooled for 3 hours. This was filtered and freeze-dried. The yield was 1.15g.

(Production Example 4) 10 g of tab powder (domestic product used in leaves) which is commercially available as a raw material for incense sticks was extracted with a Soxhlet extractor using ethanol as a solvent for 3 hours. This was filtered and freeze-dried. Yield 0.69g
Met.

(Production Example 5) 10 g of commercially available tab powder (a bark used in China as a raw material) for incense was extracted with a Soxhlet extractor using ethanol as a solvent for 3 hours. This was filtered and freeze-dried. The yield was 1.10g.

(Example 1) Lotion (parts by weight) Methanol extract of bark of Tabuki of Production Example 1 1.0 Ethanol 80.0 Purified water 19.0

(Example 2) Cream (parts by weight) A. Squalane 20.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan monostearate 2.0 Tab bark 50% methanol extract of Preparation Example 2 1.0 B. Purified water 55.9 Polyoxyethylene (20E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60E.O.) Hydrogenated castor oil 1.0 Glycerin 5.0 Methyl paraoxybenzoate 0.1 A and B Are weighed, heated to 70 ° C., gradually added A to B while stirring, and then slowly stirring at 30
Cooled to ° C.

(Example 3) A case where the methanol extract of bark of Acacia catechu of Production Example 1 of Example 1 was replaced with an aqueous extract of bark of Acacia catechu of Production Example 3.

(Example 4) The tabuki bark 50% methanol extract of Production Example 2 of Example 2 was replaced with the tab powder (domestic production and leaves used in leaves) ethanol extract of Production Example 4.

(Example 5) The methanol extract of bark bark of Example 1 of Example 1 was replaced with the ethanol extract of tab powder (Chinese bark used in China) of Example 5 of manufacture.

(Example 6) A 50% methanol extract of bark of Tabnox bark of Production Example 2 of Example 2 was replaced with an ethanol extract of Tab powder (a bark used in China).

(Comparative Example 1) The extract of Tabanuki of the production example of Example 1 was replaced with water.

(Comparative Example 2) The extract of Tabanuki of the production example of Example 2 was replaced with water and used as the B component.

For the evaluation, the tyrosinase activity inhibition rate (%) and the active oxygen production inhibitory effect (inhibition rate%) of the extracts of Production Examples 1 to 5 were measured, and Examples 1 to 6 and Comparative Example 1, Regarding No. 2, the sensory test was conducted by actual use.

(Inhibition of tyrosinase activity) Test method 0.9 ml of McClvaln buffer, 1.66 mM
1.0 ml of Tyrosine solution and 1.0 ml of sample solution were placed in a screw vial and heated in a 37 ° C. constant temperature water bath for 5 minutes or more. Add 0.1 ml of tyrosinase solution (Sigma, mushroom-derived, 914 units / ml) to 37
The temperature was kept in a constant temperature water bath at ℃, and after 10 minutes, the absorbance was measured at 475 nm. As a control, pure water was added instead of the sample solution and the same measurement was performed. (Calculation formula) Tyrosinase activity inhibition rate (%) = {B- (AP)} / B
× 100 However, A: Absorbance of sample specimen B: Absorbance of control P: Absorbance due to coloring of sample specimen (3-fold dilution) The results are shown in Table 1.

[0028]

[Table 1]

(Effect of production of active oxygen) 21 in the atmosphere
There is% oxygen, without which no organisms (except anaerobic) can exist. However, oxygen becomes active oxygen under the influence of ultraviolet rays and enzymes. Active oxygen oxidizes fatty acids and produces peroxides. It also oxidizes and damages the phospholipids of biological membranes in the body. Moreover, it is said that the generated peroxide and active oxygen damage DNA and accelerate aging. It also influences the mechanism of melanin production from tyrosine and is involved in skin darkening. Suppressing this active oxygen is one of the important factors required for the skin, in other words, for cosmetics.

There are various methods for measuring the effect of suppressing active oxygen, but the following method was used this time. pH 7.8, 50 mM potassium phosphate buffer (containing 1.3 mM DETAPAC) 133 ml 40 unit / ml catalase above potassium phosphate buffer 5 ml 2 mM nitroblue tetrazolium above potassium phosphate buffer 5 ml 1.8 mM xanthine above phosphorus Potassium acid buffer 17 ml ────────────────────────────────── 160 ml 2.4 ml of the above reagent mixture, Add 0.3 ml of the sample, and add xanthine oxidase (use water as the sample in advance and adjust the above-mentioned potassium phosphate buffer solution so that the absorbance increases about 0.02 per minute). Immediately after adding 0.3 ml, the absorbance (560 nm) is measured.
(Measurement is performed for 2 minutes and linearity is confirmed.) (Calculation formula) Inhibition rate = (AB) / A × 100 A: Change in absorbance per minute when the sample is water B: Sample Change in absorbance per minute The results are shown in Table 2.

[0031]

[Table 2]

(Use test) Faces of 10 women were divided into right and left sides, one of which was used as an example and the other of which was used as a comparative example once or more daily, and a questionnaire was given after 3 months. In addition, 30 people were divided into 3 groups and an experiment was performed using the following samples (Table 3).

[0033]

[Table 3]

The criteria are as follows, and the results of the questionnaire are summarized in Table 4. Example is very good 3 Example is much better 2 Example is slightly better 1 No difference 0 Comparative example is better -1 Comparative example is much better -2 Comparative example Is very good -3

[0035]

[Table 4]

[0036]

EFFECTS OF THE INVENTION The extract of bark and leaves of Acacia catechu is useful for whitening, preventing rough skin and preventing wrinkles, and is optimal as a raw material for cosmetics.

─────────────────────────────────────────────────── ───

[Procedure amendment]

[Submission date] December 10, 1991

[Procedure Amendment 1]

[Document name to be amended] Statement

[Name of item to be corrected] 0027

[Correction method] Change

[Correction content]

(Inhibition of tyrosinase activity) Test method 0.9 ml of McClvaln buffer, 1.66 mM
1.0 ml of Tyrosine solution, of the above sample solution
1.0 ml of 0.1 wt / v% was placed in a screw vial and heated in a 37 ° C. constant temperature water bath for 5 minutes or more. 0.1 ml of tyrosinase solution (Sigma, mushroom-derived, 914 units / ml) was added, and the mixture was kept warm in a constant temperature water bath at 37 ° C.
After 10 minutes the absorbance was measured at 475 nm. As a control
Pure water was added instead of the above sample solution, and the same measurement was performed. (Calculation formula) Tyrosinase activity inhibition rate (%) = {B- (AP)} / B
× 100 However, A: Absorbance of sample specimen B: Absorbance of control P: Absorbance due to coloring of sample specimen (3-fold dilution) The results are shown in Table 1.

[Procedure Amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0028

[Correction method] Change

[Correction content]

[0028]

[Table 1]

[Procedure 3]

[Document name to be amended] Statement

[Correction target item name] 0031

[Correction method] Change

[Correction content]

[0031]

[Table 2]

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Masao Hattori 2556-4-22-203 Gofuku Suehiro-cho, Toyama City, Toyama Prefecture (72) Inventor Kenji Shimomura 3-16-32 Funae, Ise City, Mie Prefecture (72) Inventor Nakamura Masami 6-32 Ikegami-cho, Toba-shi, Mie (72) Inventor Nishizato Neutralization 1056-1 Motomachi, Omata-cho, Dokai-gun, Mie-ken

Claims (1)

[Claims] 1. A cosmetic containing a solvent extract of bark and leaves of the genus Tabanus of the family Asteraceae.