JPH0355899Y2 - - Google Patents

Description

[Detailed Description of the Invention] (Industrial Application Field) The present invention relates to a shaking test plate used, for example, in immunological agglutination tests.

(Prior art) Immunological agglutination tests use either antigens or antibodies that cause an immunological reaction, or a combination of both, to react with a test object such as a body fluid, resulting in an antigen-antibody reaction. Based on this, the presence of immunoactive components is confirmed by observing with the naked eye or under a microscope. In this inspection,
Test reagents have been developed in which antigens or antibodies are supported on particulate carriers such as latex, allowing easy observation.

In the above-mentioned agglutination reaction test, the test object is subjected to a shaking test in which a shaking operation is performed in order to cause a sufficient reaction between the test object and the reagent. In conventional shaking tests, the test object, reagents, etc. are placed in a liquid reservoir (well) formed by a depression on the top surface of a shaking test plate, and the plate is shaken using a shaker or the like. .

The conventional shaking test plate was published in 1978.
As disclosed in Japanese Utility Model Publication No. 57-201948, the plate body has a plurality of liquid reservoirs each having an opening formed by a circular recess on the upper surface thereof. Such a shaking test plate is shaken so that the liquid in each liquid reservoir flows circularly along the wall surface. However, since each liquid reservoir is only a concave depression, the liquid located in the center of the liquid reservoir hardly flows even when the plate is shaken. As a result, the test object and reagent in the liquid reservoir are not sufficiently mixed, especially in the center of the liquid reservoir, and it takes a long time for the reaction between the two to occur. There was a problem.

(Problems to be solved by the invention) The present invention was made to solve the problems of the prior art described above, and its purpose is to ensure that the liquid in the liquid reservoir flows through shaking, and to prevent the liquid from being inspected. To provide a shaking test plate in which a body and a reagent are reliably mixed and a reaction between the two is rapidly carried out.

(Means for Solving the Problems) The shaking test plate of the present invention has a concave liquid reservoir formed on the top surface of the plate body.
A shaking test plate in which reagents, etc. are introduced and shaken, the plate projecting upward into the liquid reservoir so that its upper end is positioned approximately on the same level as or lower than the upper surface of the plate body. It has a plurality of protrusions, thereby achieving the above object.

(Example) Examples of the present invention will be described below.

As shown in FIGS. 1 and 2, the shaking test plate of the present invention has, for example, six liquid reservoirs 10 formed on the upper surface of a thin plate main body 1 that is rectangular in plan view. Each liquid reservoir 10 has a circular opening. Each liquid reservoir 10 is formed with, for example, two protrusions 11 that protrude upward. The protrusions 11 are concentric with the opening of the liquid reservoir 10, and are equally spaced on a circle whose radius is approximately 1/2 that of the opening. Each protrusion 11 has a conical shape, and its upper end is on the same plane as the upper surface of the plate main body 1 or at a position lower than it. The diameter of the protrusion 11 on the bottom surface of the liquid reservoir 10 is about 1/5 to 1/20 of the diameter of the opening of the liquid reservoir 10.

The plate body 1 may be made of plastic such as polyvinyl chloride or polypropylene, or glass.

When the shaking test plate having such a configuration is shaken in a substantially horizontal plane using a shaker or the like, the liquid in each liquid reservoir 10 generates a turbulent flow along the periphery of each protrusion 11 and flows in a complicated manner. , the liquid does not stay in the center of each liquid reservoir 10. Therefore, the test object and reagent placed in each liquid reservoir 10 are sufficiently mixed, and the reaction is promoted.

The number of protrusions 11 formed in each liquid reservoir 10 is not limited to two. For example, as shown in FIGS. 3A and 3B, three and four protrusions 11 are arranged on a circle that is concentric with the opening of each liquid reservoir 10 and whose radius is approximately 1/2. It is also possible to have a configuration in which they are equally distributed. In addition, as shown in FIGS. 3C and 3D, one protrusion 11 is provided at the center of the liquid reservoir 10, and
The two and six protrusions 11 may be arranged equally on a circle concentric with the opening of the liquid reservoir 10 and approximately 1/2 the diameter of the opening.

If each protrusion is arranged on a circle with a large radius, the fluidity of the liquid outside each protrusion will be poor during shaking, and the fluidity of the liquid inside the protrusion will also be poor, and the liquid will accumulate especially in the center. do. On the other hand, if the protrusions are arranged on a circle with a small radius, the liquid inside each protrusion will stay during shaking. Therefore, each protrusion is concentric with the opening and its radius is 1/1 of the radius of the opening.
It is preferable to arrange them on a circle with a size of about 2.

The shaking test plate of the present invention is used not only for immunological agglutination tests, but also for medical tests such as urine, blood, serum, etc., and also for testing drugs and industrial solutions.

(Effects of the invention) The shaking test plate of the invention has the following features:
Since a plurality of protrusions are formed in the liquid reservoir, the liquid, etc. in the liquid reservoir can be mixed quickly and reliably. When this shaking test plate is used for a flocculation-sedimentation reaction test, if the flocculates are large and the amount of precipitate is large, only the flocculated precipitates accumulate near the protrusions. Therefore, even if floating particles and flocculated precipitates cannot be distinguished when observing the entire liquid reservoir, the extent of the reaction can be determined by observing the vicinity of the protrusion based on the amount, size, etc. of the flocculates.

[Brief explanation of the drawing]

Fig. 1 is a plan view showing one embodiment of the shaking test plate of the present invention, Fig. 2 is a sectional view taken along the - line in Fig. 1, and Fig. 3 is a plan view showing another embodiment of the liquid reservoir. be. 1...Plate body, 10...Liquid reservoir, 11...
...projection.

Claims (1)

[Claims for Utility Model Registration] 1. A shaking test plate in which a test object, a reagent, etc. are placed in a concave liquid reservoir formed on the upper surface of the plate body and shaken, wherein the plate includes:
A shaking test plate having a plurality of upwardly protruding protrusions such that the upper end is located substantially on the same level as or lower than the upper surface of the plate body. 2. The shaking test plate according to claim 1, in which the liquid reservoir has a circular opening. 3. For shaking inspection according to claim 1 of the utility model registration claim, in which the protrusions are concentric with the opening of the liquid reservoir and are equally distributed on a circle with a radius of approximately 1/2 of the radius of the opening. plate.