JPH02109975A - Novel bacterium to decompose ethanolamines - Google Patents

Abstract

PURPOSE:To efficiently decompose ethanolamines of rust preventive, especially triethanolamine and diethanolamine by using Flavobacterium aquatile KK-111, a novel bacterium. CONSTITUTION:Flavobacterium aquatile KK-111 (FERM P-10335), a bacterium isolated from a soil in Kanazawa Ward in Yokohama City by using a separating medium containing triethanolamine as a single carbon source, capable of decomposing ethanolamines is brought into contact with a system containing a nitrogen source and an inorganic salt such as ammonium sulfate, potassium phosphate, magnesium salt and iron salt besides ethanolamines. The bacterium is cultured under an aerobic condition at pH6.0-8.0 at 15-35 deg.C until ethanolamines are sufficiently assimilated. Consequently the bacterium can be used in treating waste liquor of cutting oil containing ethanolamines.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to Flavobacterium aquatile which decomposes ethanolamines.

Ethanolamines are used in cutting fluids as rust preventive agents. The content of ethanolamines in cutting fluids varies, but can reach 40% in some cases.

[Prior Art and Problems to be Solved by the Invention] Coagulation methods, activated sludge methods, activated carbon adsorption methods, etc. are known as methods for treating cutting oil waste fluid, but these methods do not allow the ethanolamines in the waste fluid to be treated. In particular, triethanolamine and jetanolamine cannot be removed because they are difficult to decompose.

Moreover, the safety of ethanolamines to biological systems is questionable, and since they are water-soluble, once they are released into the natural environment, they diffuse widely, causing environmental pollution and making natural purification difficult. Therefore, there is a serious problem in disposing of it untreated.

[Means for Solving the Problems] Therefore, the present inventors conducted a search for microorganisms in order to decompose ethanolamines using microorganisms, and found that microorganisms isolated from soil in Kanazawa Ward, Yokohama City were found to be trivial. The present invention was achieved by discovering that ethanolamine and jetanolamine can be efficiently decomposed.

That is, the present invention utilizes Flavobacterium aquatile, which decomposes ethanolamines.
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As mentioned above, this bacterium was isolated from soil in Kanazawa Ward, Yokohama City using an isolation medium containing triethanolamine as the sole carbon source, and has the following mycological properties. There is.

A. Morphological properties 1) Cell shape and size: Bacillus 0.6X 2.0μ2
) Cell pleomorphism: None 3) Motility: None 4) Spores: Not formed 5) Durham staining: Negative 6) Acid-fastness: None B, Growth status in each medium (1) Standard agar medium (30°C, 5 days) Good growth, circular shape, flat surface ridges, smooth surface, yellow color (2) Trypto soy slant medium Growth is moderate, surface smooth, light yellow color (3) Triethanolamine agar medium (Contains 1% triethanolamine) Moderate growth, circular shape, flat surface ridges, smooth surface, light yellow color (4) Good growth on heart infusion agar medium, circular shape, yellow color C6 Physiological properties (1) Reduction of nitrate: reduced (2) Production of indole: negative (3) Production of hydrogen sulfide: negative (4) Hydrolysis of starch: positive (5) Utilization of citric acid: Christensen medium, Not used in Simmons medium (6) Lysine decarboxylation: Negative (7) Aesculin hydrolysis: Negative (8) Casein hydrolysis: Positive (9) Urealysis: Negative (10 Catalase: Positive (11) Oxidase: Positive ( 12) OF test Dioxide type (13 Production of pigment: Production of water-insoluble yellow pigment (14) Growth range: Temperature 15-30°C (15) Oxygen requirement: aerobic (1B) Sodium chloride requirement: Negative (17 ) β-galactosidase (ONPG): Negative (
18) Production of acids from sugars Glucose + 1 inositol arabinose - Sorbitol mannitol - Glycerol raffinose - Trehalose Salicin Fructose + Sucrose
+ Maltose + Xylose More properties than rhamnose in Bersey's Manual of Systematic Bacteriology, 1st edition (1984)
Based on the search, the wood fungus was confirmed to be Flavobacterium aquatile. However, none of the known bacterial strains is known to have the ability to degrade ethanolamines.

This bacterium is Flavobacterium aquatile -111
It was named FER at the Institute of Microbial Technology, Agency of Industrial Science and Technology.
It has been deposited as MP-10335. The present invention includes all mutant strains that have the ability to decompose ethanolamines, even if they are mutant strains obtained by mutating this bacterium naturally or by artificial means.

Next, in order to decompose ethanolamines by Honkan, all you need to do is bring the bacteria into contact with ethanolamines, and no special conditions are required. , a nitrogen source such as magnesium salt, iron salt, and inorganic salts.

Culture is carried out under aerobic conditions at pH 6.0-8. o, temperature 15~
The reaction is carried out at 35°C, preferably 25-30°C, until the ethanolamines are fully assimilated. The initial concentration of ethanolamines should be 0.01 to 3%, preferably 0.05 to 1%, and under these conditions, ethanolamines can be completely decomposed by culturing for about 5 to 10 days. be able to.

In addition, as a method for evaluating the decomposition of ethanolamines, especially triethanolamine and jetanolamine, by this bacterium, ethanolamines were used as the sole carbon source, and a mixture containing ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, and ferric chloride was used. Culture medium (pua, o ~ a, o) was inoculated with Miki fungi and cultured with shaking at 25 to 30°C, and the growth status, ethanolamine concentration, and total dissolved organic carbon (TOC) at the time of visit were measured over time. There is a way to measure it. Triethanolamine and jetanolamine can be efficiently decomposed in a relatively short period of time by setting the initial concentration to 1% or less.

[Example] Next, the present invention will be explained in detail by way of examples.

Example 1 Triethanolamine 0.1% in a 200m flask
, ammonium sulfate 0.1%, potassium dihydrogen phosphate 0
．． Add 100n+j of a medium (pH 7.0) containing 2% magnesium sulfate, 40ppm of ferric chloride, and 40ppm of ferric chloride, and after sterilization by a conventional method, Flavobacterium aquatile was inoculated with -111 (FERM P-10335). The cells were cultured with shaking at 30° C., and the growth state, residual amounts of triethanolamine and dissolved TOC were measured over time. The results are shown in Figure 1.

As is clear from the figure, a decrease in triethanolamine and dissolved TOC was observed as the number of visitors increased. ! 9
On the second day, the decomposition rate of triethanolamine was 99.9% or more, and the removal rate of dissolved TOC was also 92% or more.

Example 2 The same procedure as in Example 1 was conducted except that jetanolamine was used instead of triethanolamine. As a result, the decomposition rate of jetanolamine was 95% on the 6th day of culture.

[Effects of the Invention] Since the microorganism of the present invention efficiently decomposes ethanolamines, it can be used to treat cutting oil waste fluid containing the ethanolamines.

[Brief explanation of drawings]

Figure 1 shows Flavobacterium aquatile -1
11 growth states and triethanolamine and dissolved TO
It is a graph obtained by examining the relationship with C concentration over time.

Claims (1)

[Claims] Flavobacterium decomposes ethanolamines
Aqua tile.