JP7460998B2 - Skin preparations - Google Patents

Description

The present invention can be incorporated into external skin preparations or hair growth agents, has the effect of preventing and improving pigmentation of the skin (including the scalp), improving blood flow, anti-aging effect, and hair growth, and has the effect of preventing and improving pigmentation of the skin (including the scalp). The present invention relates to a new active ingredient that is less irritating to the skin (including the scalp) and has excellent biosafety.

In recent years, research has been conducted on cell aging and cell damage caused by external factors (e.g., ultraviolet rays, chemical substances such as air pollutants and environmental hormones, allergens such as pollen, environmental stress, etc.), and the mechanisms of various cell aging phenomena and cell damage and repair have been elucidated.

For example, regarding skin cells, age-related inactivation of cell proliferation and differentiation, decrease in hormone secretion, quantitative decrease in extracellular matrix components (collagen, laminin, etc.) that make up the skin, or internal factors such as poor blood circulation, etc. Aging phenomena (wrinkles) are caused by a complex interplay of external factors such as active oxygen induced by sunlight (ultraviolet rays), chemicals such as air pollutants and endocrine disruptors, allergens such as pollen, and environmental stress. , sagging, etc.), rough skin, and changes in color tone.

Furthermore, external factors such as ultraviolet rays, chemical substances, and allergenic substances damage cells and tissues within a living body, altering biological components, or generating active oxygen. As a result, antigens are generated within the cells and inflammation occurs in the living body. Furthermore, the above-mentioned external factors induce abnormal deposition of intracellular melanin pigment, causing spots, freckles, melasma, etc. on the skin.

To prevent and improve the above-mentioned cellular disorders and aging, various active ingredients have been proposed, and cosmetics, foods, beverages, and pharmaceuticals containing these active ingredients are on the market. Examples include antioxidants such as vitamin C, vitamin E, and catalase; anti-inflammatory agents such as glycyrrhizic acid or its salts, allantoin, and tranexamic acid; various ultraviolet absorbers; cell activating ingredients such as α-hydroxycarboxylic acid, placental extract, and γ-amino-β-hydroxybutyric acid; extracellular matrix components such as collagen, elastin, and hyaluronic acid or its salts; moisturizing agents such as urea; and protein glycation inhibitors such as aminoguanidine. In addition, kojic acid and linoleic acid are known as substances that suppress the occurrence of pigmentation such as skin blemishes, freckles, and melasma, and are widely used as active ingredients in skin whitening agents.

In view of the problems of the conventional technology, the present inventors have conducted intensive research to find new active ingredients derived from natural products. As a result, they have found that a hydrolysate of a plant belonging to the genus Codonopsis of the Campanulaceae family or an extract thereof has an excellent inhibitory effect against pigmentation (age spots, freckles, melasma, etc.) caused by ultraviolet rays, and further has an effect of improving blood flow, and that by incorporating the hydrolysate, it is possible to provide an external skin preparation and a hair growth agent that have excellent effects of improving the health of the skin (including the scalp), anti-aging effects, whitening effects, and hair growth effects, and that are also highly safe to the body.

It has been disclosed in, for example, Patent Document 1 that extracts of plants belonging to the genus Codonopsis in the family Campanulaceae have a whitening effect. However, it was not known that these plants or hydrolysates of their extracts have the effect of inhibiting pigmentation caused by ultraviolet rays and the effect of improving blood flow.
Patent Publication No. 2005-145888

The present invention is an external preparation for skin containing a hydrolyzate of a plant belonging to the genus Panax in the family Campanulaceae or an extract thereof as an active ingredient.
The present invention is a hair growth agent containing, as an active ingredient, a hydrolyzate of a plant belonging to the family Campanulaceae, or an extract thereof.

The present invention relates to an external skin preparation and a hair growth agent containing as an active ingredient a hydrolysate of a plant of the genus Codonopsis in the family Campanulaceae or an extract thereof. According to the present invention, by incorporating the extract as an active ingredient, it is possible to provide an external skin preparation and a hair growth agent that exerts a skin (including scalp) healthening effect, a whitening effect, and a hair growth effect.

Preferred embodiments of the present invention will now be described in detail.
In the present invention,

The plants used in the present invention belong to the Campanulaceae genus Codonopsis, and include, for example, Codonopsis lanceolata, Codonopsis pilosula, Codonopsis ussuriensis, and Condonopsis laroceolata. ) and Codonopsis tangshen.

To prepare the extract, remove the target parts of the Ginseng plant (whole plant, roots, flowers, leaves, stems, etc.) in advance by washing them with water to remove foreign substances, if necessary, and then remove them as is or after drying as necessary. This can be carried out by slicing or pulverizing the mixture and bringing it into contact with an extraction solvent according to a conventional method such as an immersion method or a countercurrent extraction method.

Extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-propanediol, 1,3-butylene glycol, and glycerin; ethyl acetate, acetic acid. Esters such as butyl and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene, and chloroform; Alternatively, two or more kinds can be used as a mixture.

When using a mixed solvent, the mixing ratio is preferably in the range of 1:10 to 20:1 by volume (same below) for a mixed solvent of water and 1,3-butylene glycol or 1,3-propanediol, 1:10 to 25:1 for a mixed solvent of water and ethanol, and 1:1 to 20:1 for a mixed solvent of water and glycerin.

Further, the weight ratio of the dried parts of each plant to the extraction solvent is preferably in the range of 1:1 to 1:80, more preferably in the range of 1:5 to 1:50.

There are no particular limitations on the pH when preparing the extract, but it is generally preferred to keep it in the range of 3 to 9. In this sense, if necessary, an alkaline regulator such as sodium hydroxide, sodium carbonate, potassium hydroxide, or an acidic regulator such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid may be added to the extraction solvent to obtain the desired effect. The pH may be adjusted to .

Extraction conditions such as extraction temperature and extraction time vary depending on the type of solvent used and pH, but for example, water, alcohols (polyhydric alcohols, lower alcohols, etc.), or a mixture of water and alcohols are used as the solvent. If so, the extraction temperature will generally range from 0°C to 90°C and the extraction time will generally range from 0.5 hours to 7 days.

In the present invention, the extract obtained by the above extraction treatment is subjected to a hydrolysis treatment before or after the extraction, or in parallel with the extraction. Examples of the hydrolysis treatment include methods using acids, alkalis, enzymes, etc., and hydrolysis treatment using enzymes is preferred. When hydrolysis is performed using an enzyme and a solvent other than water or a mixed solvent of water and a hydrophilic solvent is used to prepare the extract solution, the extraction solvent is first removed from the extract solution, The extract obtained here is preferably redissolved in water or a mixed solvent of water and a hydrophilic solvent, and then subjected to enzymatic decomposition treatment.

Examples of enzymes used in the hydrolysis treatment include proteolytic enzymes, starch-degrading enzymes, fibrinolytic enzymes, and lipolytic enzymes. These enzymes may be used alone or in combination, depending on the plant species.

Examples of proteolytic enzymes include actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, peptidases such as glycylglycine peptidase, carboxypeptidase, and aminopeptidase, bromelain, and complex protease derived from microorganisms [e.g., Neurase (manufactured by Amano Enzyme Co., Ltd.)]. When using proteolytic enzymes, any of the above enzymes may be used alone or in combination. Examples of starch-degrading enzymes include α-amylase, β-amylase, glucoamylase, and β-galactosidase. When using starch-degrading enzymes, any of the above enzymes may be used alone or in combination. Examples of cellulose-degrading enzymes include cellulase, hemicellulase, and pectinase. When using cellulose-degrading enzymes, any of the above enzymes may be used alone or in combination. Examples of lipolytic enzymes include lipase. When carrying out hydrolysis using an enzyme, one or more enzymes selected from each of the above enzyme groups may be used in combination.

Hydrolysis treatment using enzymes involves adding one or more of the above enzymes to the above-mentioned plant extract solution, and carrying out an enzymatic reaction under conditions near the optimum pH and optimum temperature of the enzyme used. It is carried out by When using two or more types of enzymes in combination, depending on the characteristics of the enzymes used, the two or more types of enzymes may be allowed to act simultaneously, or may be made to act sequentially with or without changing the reaction conditions. good. The amount of enzyme used is preferably in the range of 0.001 to 50 parts by weight, more preferably 0.1 to 10 parts by weight per enzyme per 100 parts by weight of solid content of the plant extract solution. is within the range of Further, the time for enzyme treatment varies depending on the type of enzyme used, etc., but is generally in the range of 0.5 to 24 hours, preferably in the range of 1 to 6 hours.

After hydrolysis using the above enzymes is completed, the enzymes are inactivated using an appropriate method, such as heating the enzyme-treated solution to 80°C or higher, to obtain an enzyme-treated hydrolyzate solution.

The hydrolyzed product prepared as described above may be used as it is as a cosmetic formulation after adjusting the pH to 4 to 8, or it may be concentrated to a desired concentration by vacuum concentration, etc. May be used as Further, the extract may be dried by a conventional method such as spray drying.

Moreover, in order to improve the storage stability of the hydrolyzate prepared as described above, the supernatant may be used after being refrigerated for a certain period of time.

Examples of preparations (including cosmetics and quasi-drugs) containing the hydrolysate of the present invention include milky lotions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, make-up press powders, blushers, face powders, face washes, body shampoos, hair shampoos, soaps, etc., as well as hair growth agents and bath additives, but are of course not limited to these.

In the case of basic cosmetics, the blending amount of the extract of the present invention in external skin preparations is generally 0.002 to 1.0% by weight (solid content weight%, the same hereinafter) as the solid content of the hydrolyzate, preferably In the case of makeup cosmetics, it is generally in the range of 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight. In the case of additives, the amount is generally from 0.002 to 10.0% by weight, preferably from 0.02 to 7.0% by weight. In the case of hair cosmetics or hair growth agents, the solid content of the extract is generally 0.0001 to 5.0% by weight, preferably 0.001 to 3.0% by weight. be.

In addition to the essential component, the hydrolysate, ingredients that are usually used in topical skin preparations and hair growth agents, such as oily ingredients, surfactants (synthetic and natural), moisturizers, thickeners, preservatives and disinfectants, powder ingredients, UV absorbers, antioxidants, colorants, fragrances, etc., can be added as needed. As long as the effectiveness and characteristics of the extract are not impaired, there is no problem in combining it with other physiologically active ingredients.

Here, examples of oily ingredients include lotus oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice bran oil, rice germ oil, coconut oil, chamomile oil, palm oil, cacao oil, meadowfoam oil, and rose oil. Plant-derived oils and fats such as hip oil, orchid oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, and plant-derived squalane; Animal-derived oils and fats such as mink oil and turtle oil; Beeswax, carnauba wax, and rice wax , waxes such as lanolin; hydrocarbons such as liquid paraffin, petrolatum, paraffin wax, and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and eicosenoic acid; lauryl alcohol, cetanol, stearyl Higher alcohols such as alcohol; synthetic esters such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexylglyceride, higher fatty acid octyldodecyl (octyldodecyl stearate, etc.) and synthetic triglycerides.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, and polyoxyethylene fatty acid ester. Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Anionic surfactants such as polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkylphenyl ether phosphates; quaternary ammonium salts, primary to tertiary fatty amine salts, Cationic surfactants such as alkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorphonium salts, polyethylene polyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N',N'-dimethyl-N'- Amphoteric surfactants such as β-hydroxypropylammoniosulfobetaine and the like can be used.

Emulsifiers or emulsification aids include stevia derivatives such as enzyme-treated stevia, saponin or its derivatives, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or its esters, lactose, water-soluble soybean-derived Polysaccharides, complexes of soybean-derived proteins and polysaccharides, lanolin or its derivatives, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their Derivatives, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), etc. can also be blended.

Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, striated stramonium root (white) extract, and various amino acids and their derivatives.

Examples of thickening agents include components derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; Bletilla serrata (white algae) extract; polysaccharides such as pectin, aloe polysaccharides, and Alcaligenes polysaccharides; gums such as xanthan gum, tragacanth gum, locust bean gum, and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, and acrylic acid/methacrylic acid copolymer; hyaluronic acid and its derivatives; and polyglutamic acid and its derivatives.

Examples of preservatives and disinfectants include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazolidinyl urea), polyphosphoric acid, propanediol, 1,2-pentanediol, various essential oils, bark distillate, fermented radish broth, and ethanol or 1,3-butylene glycol derived from plants such as sugar cane.

Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, grain powder (rice, wheat, corn, millet, etc.), and bean powder (soybean, adzuki bean, etc.).

Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc.

Antioxidants include, for example, butylated hydroxyanisole, butylated hydroxytoluene, propyl gallate, extract of Brussels sieboldii, extract of Bletilla serrata root, extract of peony root, vitamin E and its derivatives (e.g., vitamin E nicotinate, vitamin E linoleate, etc.), etc.

As whitening agents, t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4 - Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate) , adenosine monophosphate), and these may be blended alone or in combination.

Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include ascorbic acid ester salts such as sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and magnesium L-ascorbic acid 2-sulfate, L-ascorbic acid 2-glucoside, and L-ascorbic acid 5-glucoside. Ascorbic acid sugar derivatives such as coside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, 6-acylated products of these ascorbic acid sugar derivatives (the acyl group is a hexanoyl group, an octanoyl group, a decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, and other L-ascorbic acid tetrafatty acid esters, 3-O-ethyl ascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbate, Examples of hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), etc. Examples of tranexamic acid derivatives include tranexamic acid esters (e.g., tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester, etc.). Examples of the resorcinol derivatives include 4-n-butylresorcinol, 4-isoamylresorcinol, etc. Examples of the 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc. Examples of the nicotinic acid derivatives include nicotinamide, benzyl nicotinate, etc. Examples of the α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid, etc.

Examples of physiologically active ingredients include placenta extract, mulberry extract, saxifrage extract, perilla extract, rice bran extract or hydrolysate thereof, white mustard extract or hydrolysate thereof, fermented white mustard, peony extract or hydrolysate thereof, lactic acid bacteria fermented rice, barley extract, lotus seed extract or hydrolysate thereof, fermented lotus seed product, Job's tears hydrolysate, fermented Job's tears seed product, fermented royal jelly product, sake lees extract or ceramides contained therein, fermented sake lees product, Pandanus amaryllifolius Roxb. extract, Arcangelicia flava Merrilli extract, and chamomile extract. In addition, coral grass extract, rice leaf extract or hydrolysate thereof, eggplant (lotus, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or hydrolysate thereof, apricot fruit extract, seaweed extract such as Eucalyptus tschonoskii, extract of marine flowering plants such as Zostera marina, fermented soy milk, jellyfish water, rice extract or hydrolysate thereof, fermented rice extract, germinated rice extract or hydrolysate thereof, fermented germinated rice, black soybean extract or hydrolysate thereof, Damask rose flower extract, bamboo shoot skin extract, linoleic acid and its derivatives or processed products (e.g. liposomal linoleic acid, etc.), animal or are collagen and its derivatives derived from fish, elastin and its derivatives, glycyrrhizic acid and its derivatives (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, gamma-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, ginseng extract or its fermented product, red ginseng extract, Mitsuishi Kami extract, loofah extract, Ulva pertusa extract, peach extract, peach kernel extract, kiwi extract, sunflower extract, Zizyphus joazeiro) extract, pau d'arco bark extract, daylily extract or fermented product, hibiscus flower extract or fermented product, silverleaf extract, cherimoya extract, mango extract, mangosteen extract, funori extract, oolong tea extract, red fuki extract, striated lanthanum extract, Japanese pepper peel or seed coat extract or hydrolyzate, safflower flower extract, Casablanca extract, sweet potato extract or its fermented product, guava leaf extract, dokudami extract, banpeiyu extract, aloe extract, fig flower extract, apple extract, white asparagus extract, etc.

Next, the present invention will be explained in more detail with reference to production examples, formulation examples, and test examples, but the present invention is not limited thereto. In addition, in the following, all parts mean parts by weight, and all percentages mean weight %.

Production Example 1. Preparation of hydrolysate (1)
1000 g of purified water was added to 100 g of dried shredded roots of Codonopsis pilosula, and extracted for 12 hours at 4° C. 700 u of α-amylase was added to 1000 g of the resulting extract, which was then hydrolyzed at 55° C. for 1 hour, and then inactivated at 80° C. for 1 hour. The liquid was filtered to obtain 720 g of a pale yellow, transparent extract solution (solid concentration: 1.7%).

Production example 2. Preparation of hydrolyzate (2)
1000 g of purified water was added to 100 g of dried shredded ginseng root and extracted at 80° C. for 1 hour. 800 u of papain was added to 1000 g of the obtained extract, hydrolyzed at 40°C for 2 hours, and then enzyme deactivated at 80°C for 1 hour. The obtained extract was filtered to obtain 680 g of a transparent yellow extract solution (solid content concentration: 2.1%). Please add the enzymatic decomposition process.

Production Example 3. Preparation of hydrolysate (3)
100 g of dried shredded whole plant of Codonopsis cernua was added with 1000 g of 30% 1,3-butylene glycol aqueous solution and extracted at 80° C. for 1 hour. 1500 U of α-amylase was added to 1000 g, and hydrolysis was performed at 50° C. for 3 hours, followed by enzyme inactivation at 80° C. for 1 hour. The obtained extract was filtered to obtain 780 g of a yellowish brown transparent extract solution (solid content concentration: 2.1%).

Manufacturing example 4. Preparation of hydrolyzate (4)
1000 g of a 50% aqueous ethanol solution was added to 100 g of dried shredded roots of Salicornea herbacea, and extracted at 4° C. for 12 hours. 500 AUN of glucoamylase was added to 1000 g, hydrolyzed at 60°C for 2 hours, and then enzyme deactivated at 80°C for 1 hour. The obtained extract was filtered to obtain 820 g of a pale yellow transparent extract solution (solid content concentration: 1.9%).

Production Example 5. Preparation of hydrolysate (5)
The same procedure as in Production Example 1 was repeated except that the roots of Codonopsis lanceolata were used instead of the roots of Codonopsis lanceolata, to obtain 730 g of a yellow, transparent extract solution (solid concentration: 1.6%).

Production example 6. Preparation of hydrolyzate (6)
In Production Example 1, 680 g of a transparent yellow extract solution (solid content concentration: 2.2%) was prepared in the same manner as in Production Example 1, except that the roots of Codonopsis tangshen were used instead of the roots of ginseng. Obtained.

Prescription example 1. Lotion [Ingredients] Part Jojoba oil 1.0
Polyoxyethylene (5.5) Cetyl Alcohol 5.0
Butylparaben 0.1
Hydrolyzate of Production Example 1 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide (appropriate amount) Purified water (total amount equals 100 parts) Flavor (appropriate amount)

Formulation Example 2. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolysate of Production Example 2 was used in place of the hydrolysate of Production Example 1 contained in Formulation Example 1.

Formulation Example 3. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolysate of Production Example 3 was used instead of the hydrolysate of Production Example 1 contained in Formulation Example 1.

Prescription example 4. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolyzate of Production Example 4 was used in place of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 5. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolysate of Production Example 5 was used instead of the hydrolysate of Production Example 1 contained in Formulation Example 1.

Formulation Example 6. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolysate of Production Example 6 was used instead of the hydrolysate of Production Example 1 contained in Formulation Example 1.

Formulation Example 7. Emulsion [Ingredients] Parts Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Lotus essential oil 0.025
Polyoxyethylene (20) sorbitan monostearate 1.0
Lipophilic glyceryl stearate 1.0
Hydrogenated soy lecithin 1.5
Hydrolysate of Production Example 1 3.0
L-Ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Water-soluble collagen 0.1
Purified water (enough to make the total amount 100 parts) Flavoring (as needed)

Prescription example 8. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 7, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide contained in Formulation Example 7 were replaced with 2.0 parts of tranexamic acid. Ta.

Prescription example 9. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 7, except that 3.0 parts of arbutin was used in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide contained in Formulation Example 7. .

Formulation Example 10. Milky Lotion A milky lotion was obtained in the same manner as in Formulation Example 7, except that 5.0 parts of nicotinamide were used in place of 2.0 parts of L-ascorbic acid 2-glucoside and 0.5 parts of potassium hydroxide contained in Formulation Example 7.

Prescription example 11. Emulsion [Ingredients] Part Squalane 3.0
Behenyl alcohol 3.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) Sorbitan Monostearate 1.0
Glycerin fatty acid ester 1.0
Soybean lecithin 1.5
Hydrolyzate of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Dipotassium glycyrrhizinate 0.1
Glycerin 3.0
1,3-butylene glycol 2.0
Water-soluble collagen 0.1
Sodium hyaluronate 0.01
Purified water Amount that makes the total amount 100 parts

Prescription example 12. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 11 except that 1.0 part of tranexamic acid was used in place of 1.0 part of dipotassium glycyrrhizinate contained in Formulation Example 11.

Formulation Example 13. Lotion [Ingredients] Parts Hydrolysate of Production Example 4 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Xanthan gum 0.1
Guar gum 0.1
Fragrance Appropriate amount Potassium hydroxide Appropriate amount Purified water (total amount is 100 parts)

Formulation Example 14. Lotion A lotion was obtained in the same manner as in Formulation Example 13, except that the hydrolysate of Production Example 4 contained in Formulation Example 13 was replaced with 10.0 parts of the hydrolysate of Production Example 5.

Prescription example 15. Essence [Ingredients] Part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Hydrolyzed hyaluronic acid liquid 0.1
Hydrolyzate of Production Example 6 5.0
Citric acid 0.3
Sodium citrate 0.6
Purified water Amount that makes the total amount 100 parts

Example 16. Liquid foundation [Ingredients] Part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
Hydrolyzate of Production Example 1 5.0
Sodium carboxymethylcellulose 0.2
bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount to make the total amount 100 parts Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount

Formulation Example 17. Body Shampoo [Ingredients] Parts N-Lauroylmethylalanine Sodium 25.0
Potassium coconut oil fatty acid solution (40%) 26.0
Coconut oil fatty acid diethanolamide 3.0
Methylparaben 0.1
Hydrolyzate of Production Example 1 5.0
1,3-butylene glycol 2.0
Purified water (enough to make the total amount 100 parts)

Prescription example 18. Hair shampoo [Ingredients] Part N-coconut oil fatty acid methyltaurine sodium 10.0
Polyoxyethylene (3) alkyl ether sodium sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Coconut oil fatty acid diethanolamide 4.0
Methylparaben 0.1
Citric acid 0.1
Hydrolyzate of Production Example 2 2.0
1,3-butylene glycol 2.0
Purified water Amount that makes the total amount 100 parts

Example 19. Hair conditioner [Component A] Parts Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
Hydrolyzate of Production Example 3 2.0
1,3-butylene glycol 5.0
Purified water (enough to make the total amount 100 parts)

Prescription example 20. Hair growth agent [Ingredients] Part: Dipotassium glycyrrhizinate 0.1
Mononitroguaiacol sodium 0.02
Pyridoxine hydrochloride 0.03
l-menthol 0.8
Tamasakitsudurafuji root extract 0.3
Brown algae extract 0.3
Panax ginseng extract 0.3
Assembly extract 2.0
Hydrolyzate of Production Example 1 3.5
Trimethylglycine 0.5
Lactic acid 0.2
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Polyoxyethylene hydrogenated castor oil 0.4
L-Arginine Appropriate amount Ethanol 20.0
Purified water Amount that makes the total amount 100 parts

Test example 1. MITF gene expression evaluation test
B16 cells were prepared at 6×10 ⁵ cells/mL in Eagle's minimum essential medium containing 10% FBS, 1 mL was seeded in a φ6 cm petri dish, and cultured at 37° C. under 5% CO ₂ and saturated water vapor. After culturing for 24 hours, a culture solution containing a sample solution (hydrolysates of Production Examples 1 to 6) was added (each hydrolyzate was added so that the final concentration was 10% as a solution based on the total amount of culture solution) A theophylline-containing medium adjusted to a final concentration of 1 mM was added and cultured under the same conditions for 4 days. In addition, as a comparison, a test plot ( A control group) was established. After culturing for 4 days, the cells in each test group were collected with 1 mL of Trizol reagent (manufactured by Invitrogen). Add 200 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) to the collected cells, mix by stirring, and centrifuge for 15 minutes at 15,000 rpm and 4°C using a centrifuge (TOMY / MX-160). , 400 μL of only the aqueous layer was collected. 500 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the collected aqueous layer, mixed by stirring, and centrifuged at 15,000 rpm and 4° C. for 15 minutes to obtain a total RNA precipitate. The total RNA was washed by adding 1 mL of 75% ethanol and stirring, and centrifuged at 15,000 rpm for 15 minutes at 4°C to collect the precipitate. The recovered total RNA was subjected to a reverse transcription reaction using a specified kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) [manufactured by Takara Bio Inc.]) to synthesize cDNA. Using the synthesized cDNA as a sample, Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.) and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [manufactured by Takara Bio Inc.], The expression of the MITF gene and the internal standard β-actin gene were detected. Here, β-actin is one of the housekeeping genes (genes that are commonly expressed in a certain amount in many tissues and cells, are constantly expressed, and are essential for cell maintenance and proliferation). Since the expression level is always constant, it is used as an internal standard in PCR experiments. The test results were obtained by comparing the expression levels of the MITF gene in each test group when the expression level of the β-actin gene was held constant. In this test system, when the expression level of each gene in the control group was taken as 100, the relative value of the expression level of that gene in other test groups was determined.

The results of Test Example 1 are shown in Table 1.
[Table 1]

As shown in Table 1, the group to which the hydrolysate of the present invention was added showed an excellent effect of suppressing the expression of the MITF gene. Here, MITF acts as a transcription factor and enhances the synthesis of melanin synthesis-related enzymes such as tyrosinase, TRP1, and TRP2, and excessive synthesis of melanin synthesis-related enzymes ultimately causes pigmentation. Therefore, it is suggested that the hydrolysate of the present invention has an excellent effect of suppressing pigmentation by suppressing the expression of the MITF gene.

Test Example 2. Evaluation Test of Blood Flow Promotion Effect In this Test Example 2, an evaluation test of the blood flow promotion effect was performed using the backs of the left and right hands of seven subjects. First, the left and right hands were washed, and the subjects waited quietly in an air-conditioned room (room temperature: 20±2°C, humidity: 50±5%) for 15 minutes. Test sites of 2 cm x 2 cm were set on the backs of the left and right hands. Next, the examiner measured the initial surface temperature of each site from the front of the subject three times using a non-contact thermometer (Thermofocus, manufactured by Tecnimed Srl) and recorded the initial value. Next, 700 mL of aqueous solutions containing 10% of the hydrolysates of Production Examples 1 to 6 were prepared as test solutions, and 700 mL of aqueous solutions containing 10% of 30% 1,3-butylene glycol solution instead of the hydrolysates were prepared as a control. Each solution was placed in a plastic container (1 L) and cooled to about 15°C in advance. The subject immersed one hand in the test solution and the other hand in the control solution up to the wrist for five minutes. After five minutes, the subject raised their hand and quickly and thoroughly wiped it dry with a towel or similar. The examiner measured and recorded the surface temperature three times at 1, 3, 5, 7, 9, and 11 minutes after wiping. The change in surface temperature for each time was calculated (Δ°C) by subtracting the initial value from the average surface temperature for each time.
The results of Test Example 2 are shown in Table 2. In this Test Example, effects were observed in 6 out of 7 subjects, and the average values of the 6 subjects who observed effects are shown.

The results of Test Example 2 are shown in Table 2.
[Table 2]

As shown in Table 2, it was revealed that the skin temperature became higher when the hydrolyzate according to the present invention was added at any measurement time. Since the skin surface temperature is considered to represent the state of blood flow directly below it, the hydrolysis according to the present invention has the effect of promoting blood flow, preventing skin (scalp) aging, and It is suggested that it has a health-promoting effect.

Claims (1)

A MITF gene expression inhibitor containing as an active ingredient a hydrolyzate of Panax ginseng of the family Campanulaceae and the genus Codonopsis or a hydrolyzate of an extract of Panax ginseng.