JP6089163B1 - Beauty composition - Google Patents

Abstract

[PROBLEMS] To provide a composition useful for cosmetics, skin dry prevention / improvement, skin moisture transpiration suppression, and skin moisturizing. A composition containing ceramide and hyaluronic acid. The ceramide is preferably a plant-derived ceramide, and particularly preferably a rice-derived ceramide. The hyaluronic acid may be obtained from chicken crowns or may be derived from microorganisms. The composition can be taken as appropriate according to the purpose such as transdermal or oral. [Selection figure] None

Description

The present invention relates to a cosmetic composition, a skin dryness prevention / improving composition, a skin moisture transpiration inhibiting composition, and a skin moisturizing composition.

Ceramide is a major component of interstitial cell lipids in the outermost stratum corneum in the epidermis. Specifically, ceramide exists specifically as the main component of the stratum corneum intercellular lipid that covers the outermost layer of the skin, and plays an important role in maintaining the function of the skin as a barrier film between the living body and the outside world. Yes. The stratum corneum structure is compared to brick and mortar, and a strong barrier membrane is formed in such a way that intercellular lipids connect about 15 layers of stacked stratum corneum cells. The stratum corneum lipids are composed of approximately 50% ceramide as the main component and are composed of amphipathic lipids such as cholesterol and fatty acids. The so-called lamellar structure in which hydrophobic and hydrophilic portions are alternately repeated. It is characterized by.

It is known that ceramide can be used, for example, as an epidermal cell growth promoter and dermal external preparation (Patent Document 1) containing glucosylceramide derived from sputum, and an antiallergic agent (Patent Document 2) containing ceramide or a derivative thereof. It has been.

Hyaluronic acid, on the other hand, is a high-molecular polysaccharide present in the epidermis and dermis, cartilage, joint fluid, etc. in the skin. It retains cells based on the formation of a jelly-like matrix in the tissue, and lubricates and softens the tissue. It has functions such as sex retention.

The amount of hyaluronic acid in a living body decreases due to stress, ultraviolet rays, aging, and the like. It is known that the decrease in hyaluronic acid in the skin may cause skin malfunction such as a decrease in elasticity of the skin, a decrease in elasticity, and wrinkles, and may be related to abnormal joint function. As a direct cause of the decrease in hyaluronic acid, there are hyaluronidase (hyaluronic acid degrading enzyme) and degradation of hyaluronic acid by ultraviolet rays, and a decrease in the amount of hyaluronic acid produced in vivo. For this reason, in order to prevent, suppress, improve or normalize symptoms such as skin malfunctions and joint abnormalities, it is necessary to suppress the decrease in the amount of hyaluronic acid in vivo or to supplement hyaluronic acid.

Hyaluronic acid is produced by hyaluronic acid synthase. As mammalian hyaluronic acid synthase, Has1, Has2 and Has3 are known to exist. In particular, Has2 is a major hyaluronic acid synthase in human dermis and is said to be involved in the synthesis of high-molecular hyaluronic acid with high moisturizing power.

As what has a hyaluronic acid synthesis promotion effect | action, hyaluronic acid or its pharmaceutically acceptable salt (patent document 3), a milk basic protein fraction, and / or a milk basic protein fraction degradation product (patent document 4) Eggshell membranes (Patent Document 5) are known, but hyaluronic acid synthesis promoting action by a composition containing ceramide is not known.

In addition, cosmetics containing ceramide and hyaluronic acid (Patent Document 6) and the like are also disclosed, but only cosmetics with excellent usability are disclosed, and excellent beauty by combining ceramide and hyaluronic acid. The effect was not known.

JP 2011-195550 A JP 2003-155231 A JP 2013-180989 A JP2013-234129A JP 2014-40402 A JP-A-11-71262

An object of the present invention is to provide a cosmetic composition, a skin dryness prevention / improving composition, a skin moisture transpiration inhibiting composition, and a skin moisturizing composition containing ceramide and hyaluronic acid.

Another object of the present invention is to provide a hyaluronic acid synthesis accelerator containing ceramide.

As a result of intensive studies in view of the above problems, the present applicant has found that a composition containing ceramide has an excellent hyaluronic acid synthesis promoting effect, and has led to the present invention. Moreover, it discovered that the composition containing a ceramide and hyaluronic acid had the further outstanding hyaluronic acid synthesis promotion effect, and resulted in this invention.

The outline of the present invention is as follows.
<1> A cosmetic composition comprising ceramide and hyaluronic acid.
<2> A composition for preventing and improving dry skin, comprising ceramide and hyaluronic acid.
<3> A skin moisture transpiration inhibiting composition comprising ceramide and hyaluronic acid.
<4> A skin moisturizing composition comprising ceramide and hyaluronic acid.
<5> The composition according to any one of <1> to <4>, wherein the ceramide is a ceramide derived from rice.
<6> A hyaluronic acid synthesis accelerator characterized by containing ceramide.
<7> Hyaluronic acid synthase gene expression promoter characterized by containing ceramide.
<8> The gene expression promoter according to <7>, wherein the hyaluronic acid synthase is Has2.
<9> The composition according to any one of <6> to <8>, wherein the ceramide is ceramide derived from rice.
<10> A hyaluronic acid synthesis accelerator comprising ceramide and hyaluronic acid.
<11> A hyaluronic acid synthase gene expression promoter characterized by containing ceramide and hyaluronic acid.
<12> The gene expression promoter according to any one of <11>, wherein the hyaluronic acid synthase is Has2.
<13> The composition according to any one of <10> to <12>, wherein the ceramide is ceramide derived from rice.

According to the present invention, by containing ceramide and hyaluronic acid, by promoting the synthesis of hyaluronic acid, the moisture transpiration of the skin is suppressed, so that the moisturizing effect of the skin is enhanced and the skin is prevented from drying. And an excellent cosmetic effect can be obtained. . According to the present invention, the synthesis of hyaluronic acid can be promoted by containing ceramide.

It is a figure which shows the expression rate of the mRNA expression level which codes Has2 of Examples 1-3 and Comparative Examples 1 and 2. It is a figure which shows the expression rate of the mRNA expression level which codes Has2 of Example 2, 4 and the comparative example 1. FIG. It is a figure which shows the expression rate of the mRNA expression level which codes Has2 of Example 3, 5 and the comparative example 2. FIG.

Hereinafter, the composition of the present invention will be described. In addition, this invention is not limited to the following embodiment.

Ceramide is a kind of sphingolipid and is a general term for a group of compounds in which sphingosine and a fatty acid are amide-bonded. As ceramides, natural or synthetic ceramides are known, and they are used in various uses such as pharmaceutical uses as well as cosmetic uses for the purpose of improving functional deterioration of the stratum corneum.

The ceramide used in the composition of the present invention is derived from raw materials such as animals, plants, and microorganisms, and processed products obtained by crushing and pulverizing them may be used directly, or they may be separated and extracted. It is also possible to use the one obtained by a method such as the like or artificial synthesis. The method for extracting / separating ceramide from the raw material and the synthesis method are not particularly limited, and can be appropriately selected according to the purpose. Examples of the extraction method include a method in which an extraction solvent usually used by those skilled in the art, such as ethanol, water, and hydrous ethanol, is added, and the mixture is heated and extracted as necessary. Further, the crushing and pulverizing method for obtaining ceramide from the raw material is not particularly limited, and may be either wet or dry, and the pulverizing conditions and processing apparatus are not particularly limited, and a commercially available apparatus or the like can be used as appropriate. . Examples of the apparatus to be used include a high-pressure homogenizer, an ultrasonic pulverizer, an airflow pulverizer, a high-speed rotational impact pulverizer, a ball mill, or a bead mill. These treatments may be repeated a plurality of times as necessary so that the physical properties such as the particle diameter of the processed product are within a preferable range, or a plurality of treatments may be combined. In the present invention, among ceramides, ceramides derived from plants are preferable, and ceramides derived from rice are particularly preferable. There is no restriction | limiting in particular as content of the ceramide mix | blended with the composition of this invention, According to various conditions, such as an objective, a shape, and a use object, the content can be suitably set in a wide range. For example, it is selected in the range of 0.00001 to 50 mass%, preferably 0.00005 to 30 mass%, more preferably 0.0001 to 20 mass%.

Hyaluronic acid is a kind of glycosaminoglycan (mucopolysaccharide) and has a structure in which disaccharide units of N-acetylglucosamine and glucuronic acid are linked. The origin of the hyaluronic acid used in the present invention is not particularly limited. For example, the hyaluronic acid may be obtained from a chicken crown, may be derived from a microorganism, or may be a synthetic product. . Moreover, you may use what hydrolyzed using the enzyme etc. There is no restriction | limiting in particular in content of the hyaluronic acid in the composition of this invention, According to various conditions, such as an objective, a shape, and a use object, the content can be suitably set in a wide range. For example, it is selected in the range of 0.00001 to 50% by mass, preferably 0.0001 to 30% by mass, and more preferably 0.0001 to 10% by mass with respect to the composition of the present invention.

The composition of the present invention can be taken as appropriate according to the purpose such as transcutaneous or oral, and there is no particular limitation on the intake method, intake, number of intakes, intake time, and intake target. Moreover, it can select suitably according to various factors and objectives, such as age of an ingestion individual, a body weight, a constitution.

In addition to ceramide or ceramide and hyaluronic acid, the composition of the present invention may contain other components. When the composition of the present invention is a transdermally absorbable composition, examples of the other components include: a thickener, a moisturizer, an antiseptic, a pH adjuster, an oil and fat, a fragrance, a colorant, an antioxidant, Examples include fungicides, alcohols, nonionic surfactants, anionic surfactants, cationic surfactants, surfactants such as amphoteric surfactants, inorganic salts, lubricants, solvents, and the like. Moreover, in this invention, the chemical | medical agent, plant, etc. which are used for cosmetics, an external medicine, a quasi-drug, etc. can be used according to the objective.

When the composition of the present invention is an oral composition, examples of the other components include dietary fibers such as protein, water-soluble dietary fiber, insoluble dietary fiber, minerals, plants or plant processed products, algae, and lactic acid bacteria. Moreover, microorganisms, such as yeast, etc. can be mix | blended. In addition, sugars such as dextrin and starch, oligosaccharides, sweeteners, acidulants, colorants, thickeners, brighteners, excipients, vitamins, nutritional supplements, binding Agents, lubricants, stabilizers, diluents, extenders, emulsifiers, food additives, seasonings and the like. The content of these other components can be appropriately selected according to the form of the composition of the present invention.

The shape at the time of ingesting the composition of the present invention is not particularly limited, and when it is a percutaneous absorption composition, examples of the specific shape include skin lotion, serum, milky lotion, cream, ointment, lotion, oil. Basic cosmetics such as packs; soaps, cleansing creams, cleansing lotions, cleansing milk, facial cleansers, etc .; shampoos, rinses, treatments, etc .; hair creams, hair sprays, hair tonics, hair gels Hair lotion, hair oil, hair essence, hair water, hair wax, hair foam and other hair conditioners, hair growth and hair nourishing agents, one-part hair dyes and two-part hair dyes, hair color and other hair dyes, permanent wave agents Hair care cosmetics such as permanent agents such as hair straighteners and hair retention agents; foundations, white powder, white lipsticks, lipsticks, Makeup cosmetics such as crimson, eyeshadow, eyeliner, mascara, eyebrows, eyelashes, etc .; cosmetics for finishing such as beauty nails; Hair cosmetics, hair loss inhibitors, hair removal agents, shaving agents, sunscreen agents, acne preventing or improving agents, oily skin cosmetics, hygiene products, sanitary cotton, wet tissue and the like. The composition of the present invention may be a composition for oral cavity such as dentifrice and mouthwash. In the case of an oral composition, specific shapes include, for example, powders such as powders, granules, and fine particles, tablets (chewable), spheres, capsules, caplets, liquids, and the like. . The capsule oral composition includes soft capsules and hard capsules. In the case of an oral composition, powders such as powders, granules, and fine granules are preferred. In particular, by making a powder beverage, the processing of ceramide and hyaluronic acid is easy, and the stability as a composition is excellent. Unlike capsules and tablets, it is preferable because many compositions can be taken at once.

The compounding ratio (weight ratio) of ceramide and hyaluronic acid in the composition of the present invention is not particularly limited, and can be set as appropriate according to the conditions such as the purpose and the object of use, and ceramide: hyaluronic acid = 1: 0.01 to 1000. The ratio is preferably selected from 1: 0.05 to 500, more preferably from 1: 0.1 to 100.

Hereinafter, the present invention will be specifically described by way of examples. The present invention is not limited to these examples.

<Hyaluronic acid synthase gene expression promoting action>
Using the test substances shown in Table 1 below, the gene expression promoting action of Has2, a hyaluronic acid synthase, was confirmed. The ceramide used was ceramide derived from rice.

Normal human skin fibroblasts (manufactured by Fibrocell, product number KF-4109) were cultured in an incubator under conditions of 37 ° C. and 5% CO ₂ using 10% FBS-DMEM medium. Cells suspended by trypsin-EDTA treatment are seeded in a collagen-coated 24-well plate at 500 × L so as to be 5.0 × 10 ⁴ cells / well, and in an incubator under conditions of 37 ° C. and 5% CO ₂ . For 24 hours. After the culture, the medium was removed, 500 μL of the test substance-containing medium was added, and the cells were cultured in an incubator for 24 hours under conditions of 37 ° C. and 5% CO ₂ . After culturing, the culture supernatant is removed, washed twice with 500 μL / well of PBS, RNA is collected from the cells using an RNA extraction kit (RNeasy mini kit: QIAGEN), and the RNA is reverse transcription reaction kit CDNA was synthesized using (Quantitative Reverse Transcription Kit: QIAGEN). The obtained cDNA was diluted 10 times, and the mRNA expression level of Has2 which is a hyaluronic acid synthase was measured using the obtained cDNA. The expression level of mRNA was calculated from the following calculation formula as a ratio to the expression level of mRNA in the medium not containing the test substance.

Expression level = (mRNA expression level in medium containing test substance) / (mRNA expression level in medium not containing test substance)

As shown in FIG. 1, in Examples 1 to 3 in which only ceramide is used as a test substance, the expression level of Has2 is 1.290 to 1.548, and a higher Has2 gene expression promoting effect is obtained compared to the control. I understood it. On the other hand, in Comparative Examples 1 and 2 using hyaluronic acid, which has a known gene expression promoting action of Has2, 1.039 to 1.110. Although the gene expression promoting action of Has2 is higher than the control, It turned out that it is low compared with Examples 1-3. Moreover, from FIGS. 2 and 3, Examples 4 and 5 using ceramide and hyaluronic acid in combination are compared with the case of using the same amount of hyaluronic acid as the total amount of hyaluronic acid and ceramide (Comparative Examples 1 and 2). Not only can a high Has2 gene expression promoting effect be obtained, but also a higher Has2 gene expression promotion compared to the case where ceramide is used in the same amount as the total amount of hyaluronic acid and ceramide (Examples 2 and 3). It was found that the effect can be obtained.

From the above, ceramide can promote Has2 gene expression in a small amount as compared with hyaluronic acid even when used alone, and further, by combining ceramide and hyaluronic acid, it can synergistically promote Has2 gene expression. confirmed. Thereby, the hyaluronic acid synthesis promoter of the present invention containing ceramide has excellent Has2 gene expression promoting action, and the composition of the present invention containing ceramide and hyaluronic acid has excellent Has2. By promoting the synthesis of hyaluronic acid, it suppresses the moisture transpiration of the skin by enhancing the synthesis of hyaluronic acid, thereby enhancing the skin moisturizing effect and preventing the skin from drying out, resulting in an excellent cosmetic effect Can be obtained.

According to the present invention, the expression of Has2 is promoted, the synthesis of hyaluronic acid can be promoted, the skin moisturizing effect is enhanced, and the skin is prevented from being dried. -It can be used as an improvement composition, a skin moisture transpiration suppression composition, and a skin moisturizing composition.

Claims (2)

A hyaluronic acid synthesis accelerator comprising ceramide and hyaluronic acid, wherein a mixing ratio (weight ratio) of the ceramide and the hyaluronic acid is ceramide: hyaluronic acid = 1: 0.1 to 100 Hyaluronic acid synthesis accelerator characterized by the above . 2. The hyaluronic acid synthesis accelerator according to claim 1, wherein a mixing ratio (weight ratio) of the ceramide and the hyaluronic acid is ceramide: hyaluronic acid = 1: 2.5 to 8.3 .