KR101814538B1 - A cosmentic composition comprising the bio-cellulose powder for reducing and preventing cellulite - Google Patents

Abstract

The present invention relates to a composition containing a bio-cellulose powder as an active ingredient, and more specifically, to an anti-cellulite effect test on all subjects using the bio-cellulose of the present invention, the dermal and subcutaneous fat interface thicknesses are significantly reduced The elasticity of the femur after use of the product was significantly improved (Experimental Example 1) compared with before use of the product; In the Global Assessment of Efficacy by Patient, the subject's cellulite reduction test after the use of the test product showed significant cellulite removal and skin elasticity improvement (Experimental Example 2) It can be used as a cosmetic application for preventing smoothing and sagging. Therefore, it can be usefully used as an external dermatological pharmaceutical composition and a cosmetic composition for reducing and preventing cellulite.

Description

[0001] The present invention relates to a cosmetic composition for reducing and preventing cellulite containing a powder of bio-cellulose as an active ingredient,

The present invention relates to a cosmetic composition for reducing and preventing cellulite containing a powder of bio-cellulose as an active ingredient.

[Patent Document 1] Korean Patent Publication No. 10-2012-0090137

[Patent Document 2] Korean Published Patent Application No. 2015-0072797

[Literature 3] Method of testing primary irritant substances 38 (187): pp 1500-1541

In recent years, interest in and expectation of skin beauty has increased with the improvement of living standards, and there is a growing interest in slimming products in which the interest of women in cosmetics, especially functional beauty products,

Local obesity is an excess fat deposition in certain areas such as the abdomen and stomach, and is more common in women due to endocrine and anatomical factors. Local obesity has a direct effect on health and quality of life, and it is especially meaningful in that it causes psychological atrophy and social maladjustment in the aspect of cosmetic molding.

Lipodystrophy is caused by an excess of lipid localized in a certain region of subcutaneous connective tissue, and hyperplastic fat lobules composed of globular fat cells of 100 μm or more appear. Defects in the metabolism of the dermis begin with fibroblast proliferation and local stenosis of the venous-lymph system, resulting in collagen production, fiber proliferation enhancement, orange skin and edema. As these symptoms progress further, fibrosis and transdermal membranous bulbous diseases appear, unlike obesity, it is difficult to control diet or exercise.

Cellulite is caused by pathologies like lipodystrophy, but it is narrower than fat lipodystrophy. Cellulite is a circulatory disorder caused by the accumulation of excessive fats and waste, which causes the skin to become rugged like a rough orange skin. It is produced in the dermal layer of the skin where collagen and elastin are present, and occurs particularly frequently in women. Cellulite obesity is not only abnormal accumulation of subcutaneous adipose tissue but also lymphatic system is stagnated in the interstitial space of the human body due to abnormality of the lymphatic system, and a kind of swelling phenomenon appears at that site, and it looks like it is steamed. If the lymphatic fluid stagnates in the interstitial space, not only the cosmetic problems such as obesity but also accumulation of toxic substances in the body adversely affect the skin cells and inhibit the ionization activity for the nutrition of the cells, thereby causing not only skin aging but also cell necrosis .

As described above, it is known that the cause of fat dystrophy and cellulite is different from mere obesity (Korean Patent Laid-Open Publication No. 10-2012-0090137).

In this way, not only in terms of health, but also in the desire to improve the quality of life by social changes such as the improvement of women's social status and economic independence, interest in beautiful body is also increasing rapidly to be. Currently, many women are increasingly demanding relatively safe and cheap products such as slimming and anti-celullite cosmetics due to the psychological burden of cosmetic surgery, cost, and side effects.

On the other hand, conventional slimming body care products are merely a temporary effect of an appetite suppressant drug or diuretic, and have side effects such as dizziness, vomiting, insomnia and hallucination. Fat is easily removed by diet or exercise, but there is an increasing demand for cellulite removal slimming products because cellulite is not easy to remove.

The prior art discloses caffeine as a lipid degradation or cellulite reducing substance as disclosed in Korean Laid-Open Patent Application No. 2015-0072797, and caffeine is known to be a toxic substance, and side effects such as bronchodilation and deterioration of the premenstrual syndrome phenomenon have been reported.

Accordingly, there has been a demand for development of a natural material having a cellulite reducing effect and no side effects, or a natural material having a strong cellulite reducing effect

However, none of the above documents mention or disclose the cellulite reducing and preventing effect of containing a powder of bio-cellulose as an active ingredient

The inventors of the present invention found that the anti-cellulite effect test for all subjects using the bio-cellulose powder of the present invention significantly reduced the dermal and subcutaneous fat interface thicknesses and significantly improved the elasticity of the thigh after use of the product (Experimental Example 1); In the Global Assessment of Efficacy by Patient, the subject's cellulite reduction test after the use of the test product showed significant cellulite removal and skin elasticity improvement (Experimental Example 2) It can be used as a cosmetic application for preventing smoothing and sagging, thus completing the present invention.

In order to achieve the above object, the present invention provides a dermatological pharmaceutical composition for reducing and preventing cellulite containing a powder of bio-cellulose as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for reducing and improving cellulite containing a powder of bio-cellulose as an active ingredient.

The term " bio-cellulose " as defined herein is characterized in that it is composed of microbial cellulose, fructose and water,

More specifically, the bio-cellulose comprises 45-60% by weight of microbial cellulose, 40-55% by weight of fructose and 3-6% by weight of water, preferably 50% by weight of microbial cellulose, 45% by weight of fructose and 5% %.

Hereinafter, the present invention will be described in detail.

The bio-cellulose powder of the present invention can be produced by adding fructose and water to microbial cellulose through the following manufacturing process,

For example, a manufacturing process for producing bio-cellulose from microbial cellulose will be described in more detail.

Microbial cellulose is a microbial cellulose production strain, Preferably a strain belonging to the genus Acetobacter, more preferably a strain selected from the group consisting of Acetobacter xylinum, Acetobacter aceti, Acetobacter liquefaciens, One or more strains selected from the group consisting of Acetobacter pasteurianus and Acetobacter hansenii are inoculated into microbial cellulose production medium, preferably fruit juice containing acetic acid and sugar, and shaken or air- The microorganism cellulosic material is cultured in a fermenter such that particles or pellets of the microorganism cellulosic material are dispersed and then the microorganism cellulosic production medium is cultured Next, a semi-transparent gel-state microbial cellulose can be produced.

In addition, since the translucent gel-state microbial cellulose prepared by the above process contains the medium components and the microbial cells therein, it is possible to produce microbial cellulose gel in which the impurities are removed by immersion in water, washing and heating .

The gel-like microbial cellulose can be produced by wet-sheet of about 0.4 - 0.8 mm by pressing in a pneumatic or hydraulic press using a sponge or a filter cloth, and the wet sheet is dried and then easily pulverized , So that the microcellular cellulose wet sheet absorbs the fructose or sugar solution or the excipient so as to restore the wet sheet thickness to 2-4 mm, and the restored microbial cellulose wet sheet Is compressed again with a pneumatic or hydraulic press to obtain a bio-cellulose wet sheet having a thickness of about 0.4 to 0.8 mm.

In addition, after the bio-cellulose wet sheet is dried in hot air or freeze-dried in a drying chamber, the dried sheet is pulverized with a dry pulverizer and passed through a 30-50 mesh sieve to obtain the bio-cellulose powder of the present invention.

The bio-cellulose powder comprises: (a) a step of inoculating a microorganism-cellulose-producing strain with a pH of 2 to 5 into a microorganism-cellulose producing medium; The microbial cellulose production medium is cultured in a shaking or air lift type fermentation tank at a temperature of 10 to 50 DEG C under a condition of 50 to 500 rpm for 6 to 71 hours to produce microbial cellulose in the form of a particle or a pellet A second step of producing a culture solution produced in a dispersed form; The culture of step 2 itself is used as a hair transplantation, and the present culture is inoculated in an amount of 1 to 20% (v / v) in a microorganism cellulose production medium titrated to a pH of 2 to 5 and cultured for 12 to 24 days to give translucent A third step of obtaining a gel-state microbial cellulose; The microbial cellulose in the form of a translucent gel containing the medium components and cells in the 1 to 12 mm prepared in step 3 is immersed in water for 6 to 4 days, washed 1 to 5 times with water and incubated at 60 to 140 캜 for 10 to 120 A fourth step of removing impurities by heating for a time; A fifth step of producing a microbial cellulose wet sheet having a water content of 75 to 99% by pressing the gel-like microbial cellulose obtained in the step 4 in a thickness of about 0.2 to 1.2 mm using a sponge in a press machine; A sixth step of immersing the wet sheet obtained in the fifth step in a fructose solution for 30 to 240 minutes; A seventh step of reabsorbing the microbial cellulose immersed after the immersion in the sixth step to a thickness of 1 to 6 mm; An eighth step of producing a bio-cellulose wet sheet having a thickness of 0.1 to 1.4 mm by re-pressing the restored micro-organism cellulose of the seventh step with a compactor; A ninth step of drying the biocellulose wet sheet prepared in the eighth step in a drying chamber at 30 to 100 DEG C for 6 to 36 hours; The dried sheet of the ninth step is pulverized by a dry mill and passed through a 10 to 80 mesh sieve.

Therefore, the present invention relates to a microbial cellulase producing strain, preferably a strain of the genus Acetobacter, more preferably a microbial cellulase producing strain, such as Acetobacter xylinum, Acetobacter aceti, At least one strain selected from the group consisting of Acetobacter liquefaciens, Acetobacter pasteurianus and Acetobacter hansenii is cultured in a microorganism having a pH of 2 to 5, preferably 3 to 4, Cellulose production medium, preferably about 20 to 55% coconut water or apple juice, containing about 0.1 to 3% (v / v) acetic acid and about 1 to 30% (v / v) More preferably about 30 to 45% coconut water or apple juice; The microbial cellulose production medium is shaken at a temperature of 10 to 50 캜, preferably 20 to 40 캜, for about 6 to 71 hours, preferably 1 to 3 days, at about 50 to 500 rpm, preferably 100 to 200 rpm Or an air lift type fermenter to produce a culture solution in which microbial cellulose in the form of a particle or a pellet is dispersed; The microbial cellulose production medium, preferably about 0.1 to 3% (v / v) of acetic acid and / or acetic acid, which is suitable for microbial cellulose production using pH 2 to 5, preferably 3 to 4, about 1 to 30% (v / v) sugar, more preferably from about 0.5 to 2% (v / v) of acetic acid and from about 5 to 20% (v / v) number of coconut sugar-containing or cider, More preferably in an amount of 1 to 20% (v / v), preferably 5 to 20% (v / v) in a coconut water or apple juice diluted with about 30 to 45% Culturing the mixture for 24 days, preferably 3 days to 10 days, to obtain a translucent gel-state microbial cellulose; The microbial cellulose in a translucent gel containing the medium components and the microorganism within about 1 to 12 mm, preferably 5 to 8 mm in thickness, prepared in the above step 3 is immersed in water for about 6 hours to 4 days, Followed by washing with water for 1 to 5 times, preferably 1 to 3 times, and heating at 60 to 140 占 폚, preferably at 80 to 120 占 폚 for about 10 minutes to 120 hours, preferably 20 to 60 minutes A fourth step of removing impurities; The gel-like microorganism cellulose obtained in the step 4 is compressed in a compression machine using a sponge to a thickness of about 0.2 to 1.2 mm, preferably about 0.4 to 0.8 mm to obtain a water content of 75 to 99%, preferably 85 - a fifth step of producing a 95% microbial cellulose wet sheet; A wetting agent obtained by immersing the wet sheet obtained in the fifth step in a fructose solution, preferably 1% to 20%, more preferably about 3% to 10% fructose solution for 30 to 240 minutes, preferably 60 to 120 minutes Step 6; A seventh step of reabsorbing the immersed microbial cellulose after the immersion in the sixth step to restore its thickness to 1 to 6 mm, preferably 2 to 4 mm; An eighth step of producing a bio-cellulose wet sheet having a thickness of 0.1 to 1.4 mm, preferably 0.4 to 0.8 mm by re-pressing the restored micro-organism cellulose of the seventh step with a compactor; A ninth step of drying the biocellulose wet sheet produced in the eighth step in a drying room at 30 to 100 DEG C, preferably 50 to 80 DEG C for 6 to 36 hours, preferably 12 to 24 hours; The dried sheet of the ninth step is pulverized by a dry pulverizer and passed through a 10 to 80 mesh, preferably 30 to 50 mesh sieve, to obtain a bio-cellulose of the present invention A method for producing a powder is provided.

(A) a microorganism cellulosic producing strain, preferably an Acetobacter strain, more preferably an acetolactone-producing strain, and more preferably, From the group consisting of Acetobacter xylinum, Acetobacter aceti, Acetobacter liquefaciens, Acetobacter pasteurianus and Acetobacter hansenii. One or more selected strains are cultivated in a microbial cellulose production medium suitably pH 2 to 5, preferably 3 to 4, preferably acetic acid and sugar, about 0.1 to 3% (v / v) to about 20 to 55% coconut water or apple juice, more preferably about 30 to 45% coconut water or apple juice, containing sugar, v / v; The microbial cellulose production medium is shaken at a temperature of 10 to 50 캜, preferably 20 to 40 캜, for about 6 to 71 hours, preferably 1 to 3 days, at about 50 to 500 rpm, preferably 100 to 200 rpm Or an air lift type fermenter to produce a culture solution in which microbial cellulose in the form of a particle or a pellet is dispersed; The microbial cellulose production medium, preferably about 0.1 to 3% (v / v) of acetic acid and / or acetic acid, which is suitable for microbial cellulose production using pH 2 to 5, preferably 3 to 4, about 1 to 30% (v / v) sugar, more preferably from about 0.5 to 2% (v / v) of acetic acid and from about 5 to 20% (v / v) number of coconut sugar-containing or cider, More preferably in an amount of 1 to 20% (v / v), preferably 5 to 20% (v / v) in a coconut water or apple juice diluted with about 30 to 45% Culturing the mixture for 24 days, preferably 3 days to 10 days, to obtain a translucent gel-state microbial cellulose; The microbial cellulose in a translucent gel containing the medium components and the microorganism within about 1 to 12 mm, preferably 5 to 8 mm in thickness, prepared in the above step 3 is immersed in water for about 6 hours to 4 days, Followed by washing with water for 1 to 5 times, preferably 1 to 3 times, and heating at 60 to 140 占 폚, preferably at 80 to 120 占 폚 for about 10 minutes to 120 hours, preferably 20 to 60 minutes A fourth step of removing impurities; The gel-like microorganism cellulose obtained in the step 4 is compressed in a compression machine using a sponge to a thickness of about 0.2 to 1.2 mm, preferably about 0.4 to 0.8 mm to obtain a water content of 75 to 99%, preferably 85 - a fifth step of producing a 95% microbial cellulose wet sheet; A sixth step of drying the wet sheet obtained in the fifth step in a drying chamber at 30 to 100 캜, preferably 50 to 80 캜, for 6 to 36 hours, preferably 12 to 24 hours; And a seventh step of pulverizing the dried sheet of the sixth step with a dry pulverizer and passing the pulverized product through a sieve of about 10 to 80 meshes, preferably 30 to 50 meshes, The present invention also provides a manufacturing method for producing a powder.

Accordingly, the present invention provides an external dermatological pharmaceutical composition and a cosmetic composition for reducing or preventing cellulite containing the bio-cellulosic powder prepared by the above-described method and the above-described method as an active ingredient.

The biocellulose powder contained about 50.2% of dietary fiber within 5 g of the dried cellulose powder obtained through the above-mentioned manufacturing process. The bio-cellulose is characterized by being composed of microbial cellulose, fructose and water, Specifically, the bio-cellulosic is composed of 45-60% by weight of microbial cellulose, 40-55% by weight of fructose and 3-6% by weight of water, preferably 50% by weight of microbial cellulose, 45% by weight of fructose and 5% .

The skin cosmetic composition containing the powder of the bio-cellulose as an active ingredient is characterized by containing 0.1 to 50% by weight based on the total weight of the composition.

The present inventors have significantly reduced the dermal and subcutaneous fat interface thicknesses through the anti-cellulite effect test on all subjects using the bio-cellulose powder of the present invention, and compared the results before use, the elasticity of the femoral component was significantly (Experimental Example 1); In the Global Assessment of Efficacy by Patient, the subject's cellulite reduction test after the use of the test product showed significant cellulite removal and skin elasticity improvement (Experimental Example 2) It can be used as a cosmetic application for preventing smoothing and sagging. Thus, it can be used as an external dermatological pharmaceutical composition and a cosmetic composition for reducing and preventing cellulite.

Accordingly, examples of some formulations of cosmetics and medicines containing the composition of the present invention are presented below, but the present invention is not limited thereto. The functional cosmetic composition or pharmaceutical composition of the present invention may be in the form of a solution, suspension, emulsion, ointment, paste, gel, cream, lotion, powder, spray, pack, skin patch, dressing, , Surfactant-containing cleansing, oil, etc., in any manner known in the art to obtain the best possible formulation.

In addition, the bio-cellulose powder of the present invention has no problems such as toxicity and side effects, and has proved to be a non-irritant sample in the skin patch test, so that it can be safely used for long-term use.

The pharmaceutical composition may be in the form of a cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta, cataplasma, solution, May be manufactured in any manner known in the art to obtain the best possible formulation in the form of patches, dressings, hydrodynamic or non-hydrodynamic patches, surfactant-containing cleansing oils, etc., but is not limited thereto .

The cosmetic composition may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, a foundation, Such as soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cream, body oil, body cleanser, shampoo, ointment, patch, lotion, skin, lotion, body, facial mask, etc. Pack, and may be manufactured in any manner known in the art, but is not limited thereto.

In addition to the above-described ingredients, the external preparation for skin of the present invention can be blended with various known ingredients to be incorporated in cosmetics applied to skin or mucous membranes, medicines for external use, and quasi-drugs.

The composition of the present invention can be applied by applying an appropriate amount to the skin according to the skin area to be applied, and may be used once or several times per day as needed. The amount and frequency to be applied can be appropriately increased or decreased according to the necessity according to the skin condition of an individual, formulation, age, sex, weight, obesity, degree of symptom, reaction sensitivity, application period,

When the composition of the present invention is administered as a medicament, a person skilled in the art can appropriately select an effective dose for the desired effect, for example, an effective amount is 0.1 mg to 5000 mg per one day of skin surface area (cm 2) Within the range of 100 mg to 500 mg, it may be applied 1 to 5 times a day. The dosage form of the composition may be a single dose or a repeated dosage form, and the effective daily dose may be administered in several divided doses. The dose is not intended to limit the scope of the invention in any way.

The pharmacological or cosmetic composition of the present invention may be used for the purpose of enhancing the permeability of an active ingredient, such as iontophoresis, sonophoresis, electroporation, microelectrical patch, The composition may be applied to the skin by means of pressure, osmotic gradient, occlusive cure or microinjection therapy, or a combination of these.

The pharmaceutical or cosmetic composition of the present invention may be used as a sole therapy, but it can be used in combination with exercise therapy, liposuction, hormone therapy, medication, diet, and the like.

In the composition of the present invention, the skin absorption rate of the active ingredient is 0.01 μg / cm 2 to 150 μg / cm 2 per hour. Preferably, the skin absorption rate of the cosmetic composition of the present invention is 0.1 μg / cm 2 to 100 μg / cm 2 per hour, more preferably 1.0 μg / cm 2 to 50 μg / cm 2 per hour. When the skin absorption rate is less than 0.01 μg / cm 2 per hour, the cellulite improving effect is insufficient. When the skin absorption rate exceeds 150 μg / cm 2 per hour, the cellulite improving effect / body slimming effect is insufficient It is not economical.

Examples of products to which the present composition can be added include cosmetics such as lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, pack, cleansing, cleanser, soap, have.

The cosmetic composition of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) , Derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetic acid, dl-alpha tocopherol nicotinic acid vitamin E, dl-pantothenyl alcohol, D-pantothenyl alcohol, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients.

Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearoyl hydroxystearic acid isostearyl, 12-stearoyl stearyl hydroxystearate, 12-stearo And monohydroxystearic acid and esters such as sostearyl.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino- p- (carbo-2'-ethylhexyl-1'- , 3,5-triazine, 2- (2- And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above components may be blended within the range not to impair the objects and effects of the present invention, but it is preferably 0.01 to 5% by weight based on the total weight, Preferably 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The components contained in the cosmetic composition of the present invention may contain, as an active ingredient, the components commonly used in cosmetic compositions in addition to the above-mentioned bio-cellulose powders. For example, conventional ingredients such as stabilizers, solubilizers, vitamins, Phosphorus auxiliaries and carriers.

The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences, and hair cosmetics.

Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The anti-cellulite effect test on all subjects using the bio-cellulose powder of the present invention significantly reduced the dermal and subcutaneous fat interface thickness and significantly improved the elasticity of the thigh after use of the product compared to before use Example 1); In the Global Assessment of Efficacy by Patient, the subject's cellulite reduction test after the use of the test product showed significant cellulite removal and skin elasticity improvement (Experimental Example 2) It can be used as a cosmetic application for preventing smoothing and sagging. Therefore, it can be usefully used as an external dermatological pharmaceutical composition and a cosmetic composition for reducing and preventing cellulite.

Below, The present invention will be described in detail by the following examples and experimental examples.

However, the following examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited by the following examples and experimental examples.

Example 1. Preparation of Powder of Bio-Cellulose

The bio-cellulose of the present invention can be prepared by adding fructose and water to the micro-organism cellulose. To prepare micro-organism cellulose, coconut water is diluted to 35% (v / v) (Acetobacter xylinum) was inoculated in a 1 L Erlenmeyer flask, and the mixture was shaken at 30 DEG C and 150 rpm for 1 day to 3 days. Then, 35% containing 10% (v / v) and 10% (v / v) Was inoculated in a 1-liter beaker containing 400 ml of a coconut water dilution (pH 3-4) at 5% (v / v), and cultured at 30 ° C for 5 to 8 days.

The 5 to 8 mm-thick semi-transparent gelatinous microbial cellulose prepared through the above process contains the medium components and the microbial cells therein. Therefore, it is immersed in water for 1-2 days, cleaned 1-2 times with clear water, For 20-60 minutes to remove impurities. The gel-like microbial cellulose with impurities removed is compressed in a pneumatic press using a sponge to a thickness of about 0.4-0.8 mm to produce a micro-cellulosic cellulose wet sheet having a moisture content of 85-95% Immerse in 3% - 10% fructose solution for 60 - 120 minutes. After immersion, the microbial cellulose reabsorbs the fructose solution and is restored to a thickness of 2-4 mm. The recovered microbial cellulose is compressed again with a pneumatic press to produce a biocellulose wet sheet having a thickness of about 0.4 to 0.8 mm.

The prepared bio-cellulose wet sheet was dried in a drying chamber at 50 to 80 ° C. for 12 to 24 hours. The dried sheet was pulverized by a dry pulverizer and passed through a 30-50 mesh sieve to obtain a bio-cellulose powder (hereinafter referred to as BC) .

The composition of the bio-cellulose prepared through the above procedure is shown in Table 1, and this bio-cellulose was used as a sample of the following clinical example.

ingredient % Microbial cellulose 50.2 Fructose 45.9 moisture 3.9 Fiber diameter (㎛) <300

Example 2. Preparation of composition containing bio-cellulose powder

Purified water (water-soluble solvent) and acrylate (thickener) were added to 0.01 to 10% of the bio-cellulose of Example 1 and dissolved at a temperature of 65 to 70 ° C. The lysate was cooled, and ethanol (oily solvent) was added at about 50 ° C, neutralized with triethanolamine (neutralizing agent) at 45 ° C, and emulsified at about 2,000 rpm for 5 to 10 minutes. Then, the remaining components were added, and the mixture was stirred at about 3,300 rpm for 10 minutes. The mixture was cooled to 35 DEG C and filtered at 200 mesh to prepare a composition containing a bio-cellulose powder (hereinafter referred to as BCC).

No ingredient Content (%, w / w) function One  The bio-cellulose powder of Example 1 0.01 to 10% Active ingredient 2 ethanol 8 solvent 3 Mentyllactate 2 Skin conditioner 4 Acrylate One Incrementer 5 Triethylamine 0.5 A thickening agent 6 Spices 0.5 Spices 7 Purified water Quantity solvent sum 100

Experimental Example  1. Evaluation of Clinical Trials - Slimming  Effect evaluation

In this study, 20 female subjects aged 21 to 45 years (mean age 31.0 years) with femoral topical obesity and cellulite were selected through a history examination and skin condition diagnosis (Method of testing primary irritant substances 38 (187): pp 1500-1541).

The test product used was the BCC formulation prepared according to Example 2 above.

The subjects were allowed to apply the test article to the thighs once a day (evening) for four weeks. The amount of hypodermic fat reduction was evaluated by measuring the length of the dermis and subcutaneous fat interface between the thighs before the use (0 weeks), 2 weeks and 4 weeks after using the product. In addition, the cellulite removal and elasticity improvement effect was evaluated by the visual inspection by the inspectors, and the questionnaires of skin deflection and smoothness items were evaluated.

1-1. Experimental Method

The thickness of the interface between the dermis and the subcutaneous fat was measured at 15 cm above the knee using a Dermascan C ultrasound system. Measurements were made before use, at 2 weeks and 4 weeks after use of the product, and the same area was measured using a transparent sheet showing the position at the time of initial measurement, and then the control was analyzed. Statistical significance was assessed by comparing the mean values of the measurements (in mm) obtained from 10 subjects at each time point and before and after using the Student t-test on both sides (significance level: p = 0.05 ). The results of the analysis are shown in Table 3 below.

The cellulite layer thickness measurements of the femur before use, after 2 weeks of use, and after 4 weeks of use were analyzed and found to be 5913.05 ± 1032.24 (μm) before use, After 5 weeks, 5368.13 ± 1203.55 (μm) and 5017.13 ± 1190.28 (μm) after 4 weeks of use, respectively.

In order to clearly understand the decreasing tendency of the thickness of the thigh cellulite layer, the significance of the difference between the mean and the mean was determined by a statistical method. Statistical analysis was performed using the Paired sample's T-test, a parametric method, and the Wilcoxon signed rank test, a non-parametric method. The results were statistically significant at 2 weeks and 4 weeks after use, Significant differences were found (Table 3).

One- 2. Experiment  result

As a result, when the test product of the present invention was applied two weeks after the application, the thickness of the interface decreased by about 8.8% and decreased by about 12% after 4 weeks, .

(Mean ± SD) visit Thigh Cellulite Layer Thickness 0 weeks 5913.05 占 1032.24 (占 퐉) 2 weeks 5368.13 占 1203.55 (占 퐉) 4 weeks 5017.13 占 1190.28 (占 퐉) In-group comparison Probability of significance 0 weeks to 2 weeks 0.011+ 0 weeks to 4 weeks 0.000 * *: p < 0.05 by Paired samples ' T-Test
+: p < 0.05 by wilcoxon signed rank Test

The results of visual evaluation showed that the elasticity of the thighs was significantly improved and the cellulite decreased significantly after 4 weeks of using the product compared to before use.

Experimental Example 2. Global Assessment of Efficacy by Patient

In this study, 20 female subjects aged 21 to 45 years (mean age 31.0 years) with femoral topical obesity and cellulite were selected through a history examination and skin condition diagnosis (Method of testing primary irritant substances 38 (187): pp 1500-1541).

The test product used was the formulation prepared according to Example 2 above.

(Very good), 3 points (good), 2 points (normal), 1 point (poor), and 1 point (poor) in the degree of cellulite reduction experienced by the testee after using the test product, , And 0 point (very bad). The researchers determined the efficacy of the test product by obtaining the percentage of the subjects for each answer.

The mean and standard deviation of the questionnaire values and the percentage of the subjects to each answer were obtained for the degree of cellulite reduction after using the test product.

As a result of the above-mentioned experiment, most of the subjects gave more than average answers and positively evaluated cellulite reduction (Table 4).

Results of the questionnaire survey Number of subjects (%,%) * Standard Deviation 4 3 2 One 0 Cellulite reduction 3 (15.0%) 15 (75.0%) 1 (5.0%) 1 (5.0%) 0 (0.0%) 0.34 * 4 points (very good), 3 points (good), 2 points (normal), 1 point (poor), 0 points (very bad)

Since the composition of the present invention exhibits significant cellulite removal and skin elasticity-improving effects, it can also be used for cosmetic purposes to smooth skin texture and prevent sagging.

Hereinafter, formulations of cream, massage cream, lotion, skin lotion, essence, pack, and cleansing foam are exemplified as the formulation examples of the present invention, but the formulations including the cosmetic composition of the present invention are not limited thereto.

Formulation Example  1. Cream composition

The oil phase and water phase are heated to 75 ° C and cooled to room temperature.

Formulation Example  2. Massage Cream  Composition

The oil phase and water phase are mixed by heating at 75 DEG C and then cooled to room temperature.

Formulation Example  3. lotion composition

The oil phase and water phase are mixed and emulsified by heating at 75 ° C and then cooled to room temperature.

Formulation Example  4. Skin lotion composition

The water phase and the ethanol phase are respectively prepared and mixed and then filtered.

Formulation Example  5. Essence composition

The water phase and the ethanol phase are respectively prepared and mixed and then filtered.

Formulation Example  6. Pack composition

The water phase and the ethanol phase are dispersively dissolved and mixed, and then cooled to room temperature.

Formulation Example  7. Cleansing Foam  Composition

The water phase and the oil phase are dispersed and dissolved, mixed and sieved, and then cooled to room temperature.

Claims (4)

A group consisting of Acetobacter xylinum, Acetobacter aceti, Acetobacter liquefaciens, Acetobacter pasteurianus, and Acetobacter hansenii. One or more microbial cellulose producing strains selected from the group consisting of 20 to 55% coconut water containing 0.1 to 3% (v / v) acetic acid and 1 to 30% (v / v) A first step of inoculating microorganism cellulose production medium composed of juice; The microbial cellulose production medium is cultured in a shaking or air lift type fermentation tank at a temperature of 10 to 50 DEG C under a condition of 50 to 500 rpm for 6 to 71 hours to produce microbial cellulose in the form of a particle or a pellet A second step of producing a culture solution produced in a dispersed form; The culture of step 2 itself is used as a hair transplantation, and the present culture is inoculated in an amount of 1 to 20% (v / v) in a microorganism cellulose production medium titrated to a pH of 2 to 5 and cultured for 12 to 24 days to give translucent A third step of obtaining a gel-state microbial cellulose; The microbial cellulose in the form of a translucent gel containing the medium components and cells in the 1 to 12 mm prepared in step 3 is immersed in water for 6 to 4 days, washed 1 to 5 times with water and incubated at 60 to 140 캜 for 10 to 120 A fourth step of removing impurities by heating for a time; A fifth step of producing a microbial cellulose wet sheet having a water content of 75 to 99% by pressing the gel-like microbial cellulose obtained in the step 4 in a thickness of about 0.2 to 1.2 mm using a sponge in a press machine; A sixth step of immersing the wet sheet obtained in the fifth step in a fructose solution for 30 to 240 minutes; A seventh step of reabsorbing the microbial cellulose immersed after the immersion in the sixth step to a thickness of 1 to 6 mm; An eighth step of producing a bio-cellulose wet sheet having a thickness of 0.1 to 1.4 mm by re-pressing the restored micro-organism cellulose of the seventh step with a compactor; A ninth step of drying the biocellulose wet sheet prepared in the eighth step in a drying chamber at 30 to 100 DEG C for 6 to 36 hours; Wherein the dried sheet of the ninth step is pulverized by a dry mill and passed through a 10- to 80-mesh sieve. The cosmetic composition for reducing or preventing cellulite containing the bio-cellulose powder as an active ingredient. delete delete The method according to claim 1,
The cosmetic composition may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizing cream, a hand cream, a foundation, Formulations of packs for soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions, body creams, body oils, body cleansers, shampoos, ointments, patches, lotions, skins, lotions, / RTI &gt;