JP6055667B2 - Collagen production promoter - Google Patents

Description

  The present invention relates to a melanin production inhibitor, a collagen production promoter, a skin cosmetic, and a food and drink.

  Melanin also plays a role in protecting the body from ultraviolet rays in the skin, but overproduction and uneven accumulation cause skin darkening and spots. In general, melanin is converted from tyrosine to dopa and from dopa to dopaquinone by the action of the enzyme tyrosinase biosynthesized in pigment cells, and then formed through intermediates such as 5,6-dihydroxyindophenol. ing. Therefore, in order to prevent, treat, or improve skin darkness (skin pigmentation), spots, buckwheat, etc., it is conceivable to suppress the production of melanin.

  From such a point of view, conventionally, as one having an inhibitory action on melanin production, for example, an extract from roots of castor bean (see Patent Document 1), an extract from a plant belonging to the genus Southus (Saussurea) (see Patent Document 2) Etc. are known.

  The skin is composed of the epidermis, basement membrane and dermis. The dermis is composed of fibroblasts and an extracellular matrix such as collagen, elastin and hyaluronic acid secreted from the cells. In young skin, these skin tissues maintain their homeostasis, thereby ensuring moisture retention, flexibility, elasticity, and the like, and the skin is kept fresh and fresh with a firmness and gloss.

  However, when the skin is affected by external factors such as ultraviolet rays, significant air drying, excessive skin washing, stress, smoking, etc., or when aging progresses, production of collagen, which is a major component of the extracellular matrix, is produced. As the amount decreases, it causes degradation and alteration. As a result, the moisturizing function and elasticity of the skin are reduced, abnormal exfoliation of the keratin occurs, and changes accompanying aging of the skin, that is, wrinkles, dullness, disappearance of texture, reduced elasticity, and the like occur.

  As described above, changes associated with aging of the skin, that is, formation of wrinkles, loss of tension, decrease in elasticity, and the like are associated with reduction and degeneration of extracellular matrix components such as collagen. Therefore, promoting the production of collagen is important in preventing or improving skin aging.

  Collagen is also present in a large amount in bones, tendons, ligaments, corneas, blood vessels and the like, and it is known that a decrease in collagen production due to aging or the like causes osteoporosis and the like. Furthermore, it is known that in the wound healing process, the amount of collagen produced is increased and becomes a scaffold for fibroblasts to promote wound healing. Therefore, promoting the production of collagen is important from the viewpoint of preventing or treating osteoporosis and the like, and promoting wound healing.

  Conventionally, what has a collagen production promotion effect is known, for example, an extract from a leaf part of a quince (see Patent Document 3), an extract from Kusunohagashi (see Patent Document 4), and the like.

JP 2001-213757 A JP 2002-201222 A JP 2002-226323 A JP 2003-146837 A

  The present invention finds a highly safe natural product having a melanin production inhibitory effect or a collagen production promoting effect, and contains a melanin production inhibitor and a collagen production promoter containing the natural product as an active ingredient. It aims at providing the skin cosmetics and food / beverage products which were made.

  In order to solve the above-mentioned problems, the melanin production inhibitor and the collagen production promoter of the present invention are characterized by containing an extract from pineapple and an extract from betel grass as active ingredients. Moreover, the skin cosmetics and food / beverage products of this invention mix | blended the extract from a pineapple and the extract from a quince mushroom.

  According to the present invention, there are provided a highly safe melanin production inhibitor having an excellent melanin production inhibitory action, and a highly safe collagen production promoter having an excellent collagen production promotion action and high safety. Can do.

Embodiments of the present invention will be described below.
The melanin production inhibitor and the collagen production promoter of this embodiment contain an extract from pineapple and an extract from betel grass as active ingredients. Moreover, the skin cosmetics and food / beverage products of this embodiment mix | blend the extract from a pineapple and the extract from a quince mushroom.

  Here, the “extract” in the present embodiment includes an extract obtained using the above plant as an extraction raw material, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or these Either a crude product or a purified product is included.

  In this embodiment, the extraction raw material used in order to manufacture the extract from a pineapple is a pineapple (scientific name: Ananas comosus (L.) Merr. Or Ananas sativus Schult).

  Pineapple (Ananas comosus (L.) Merr. Or Ananas sativus Schult) is a perennial plant belonging to the genus Ananas of the pineapple family native to tropical America. Pineapples are widely cultivated from tropical to subtropical regions such as Thailand, the Philippines, and Brazil, and can be easily obtained from these regions. Examples of the constituent parts of the umbilical cane that can be used as the raw material for extraction include the above-ground parts such as leaf parts, stem parts, fruit parts, flower parts, underground parts such as root parts, underground stem parts, or a mixture of these parts. However, it is preferably a fruit part.

  Moreover, in this embodiment, the extraction raw material used in order to manufacture the extract from Buttercups is a Butterflies (Scientific name: Osmanthus fragrans var. Aurantiacus).

  Buttercup (Osmanthus fragrans var. Aurantiacus) is an evergreen small tree belonging to the genus Moxae, also known as Katsura, which is native to southern China and can be easily obtained from such areas. Examples of the constituent parts of the cypress that can be used as the raw material for extraction include the aerial parts such as leaves, branches, bark parts, trunk parts, stem parts, fruit parts, flower parts, root parts, or a mixture of these parts. However, it is preferably a flower part.

  Here, the "flower" generally refers to the whole organ involved in sexual reproduction of a seed plant, and is composed of a flower leaf that is a leaf deformation and a flower axis that is a stem deformation. This includes organs such as pupae, petals, stamens, and heart skin. The “floral part” used as an extraction raw material in the present invention includes all of the organs involved in sexual reproduction of seed plants, as well as parts thereof, such as flower leaves, flower coats (buds and corolla), corolla, petals, etc. Is also included.

  The extract from pineapple and the extract from cinnamon can be obtained by drying the raw material for extraction and pulverizing it as it is or using a crusher, and subjecting it to extraction with an extraction solvent. Drying may be performed in the sun or using a commonly used dryer. Moreover, after performing pretreatment, such as degreasing, with a nonpolar solvent such as hexane, it may be used as an extraction raw material. By performing a pretreatment such as degreasing, the extraction material can be efficiently extracted with a polar solvent.

  As the extraction solvent, it is preferable to use a polar solvent, and examples thereof include water and hydrophilic organic solvents. These are used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. It is preferable.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, and those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, the water that can be used as the extraction solvent in this embodiment includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of hydrophilic organic solvents that can be used as extraction solvents include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when a mixed liquid of water and lower aliphatic alcohol is used as an extraction solvent, the mixing ratio of water and lower aliphatic alcohol is preferably 9: 1 to 1: 9 (volume ratio), More preferably, it is 7: 3 to 2: 8 (capacity ratio). In addition, when a mixed liquid of water and a lower aliphatic ketone is used, the mixing ratio of water and the lower aliphatic ketone is preferably 9: 1 to 2: 8 (volume ratio). When using the liquid mixture with a monohydric alcohol, it is preferable that the mixing ratio of water and a polyhydric alcohol is 5: 5 to 1: 9 (volume ratio).

  The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted in the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent 5 to 15 times (mass ratio) of the extraction raw material, the soluble components are extracted at room temperature or under reflux, and then filtered to remove the extraction residue. A liquid can be obtained. When the solvent is distilled off from the obtained extract, a paste-like concentrate is obtained, and when this concentrate is further dried, a dried product is obtained.

  In addition, although the extract obtained as mentioned above can be used as an active ingredient of a melanin production inhibitor or a collagen production promoter as it is, a concentrated solution or a dried product is easier to use.

  In addition, since the extract from pineapple and the extract from cinnamon have a unique odor, it is possible to perform purification for the purpose of decolorization, deodorization, etc. within a range that does not cause a decrease in the physiological activity. However, since it is not used in a large amount when blended into skin cosmetics, there is no practical problem even if it is not purified.

  Here, the extract from pineapple used in the present embodiment preferably contains 2% by mass or more, more preferably 3 to 25% by mass, of glucosylceramide as a constituent component. By containing glucosylceramide at such a content, the synergistic effect with the cinnamon extract is more prominent, and more excellent melanin production inhibitory action and collagen production promoting action can be exhibited.

[Melanin production inhibitor, collagen production promoter]
The extract from pineapple and the extract from Buttercup that are obtained as described above exhibit a synergistic effect by using them together rather than using them alone, and have excellent melanin production inhibitory action and collagen Shows production promotion effect. Therefore, the combined use of an extract from pineapple and an extract from cinnamon can be used as active ingredients of a melanin production inhibitor and a collagen production promoter. The melanin production inhibitor or collagen production promoter of this embodiment can be used for a wide range of applications such as pharmaceuticals, quasi drugs, and cosmetics.

  In addition, in this embodiment, it is preferable that the content ratio of the extract from a pineapple and the extract from a cinnamon is 1: 1-1: 100 by mass conversion, and is 1: 3-1: 20. More preferably. Moreover, when the extract from the pineapple contains glucosylceramide, the content ratio of the glucosylceramide contained in the pineapple extract and the extract from cinnamon is 1: 1 to 1: 250 in terms of mass. Preferably, it is 1:15 to 1: 100. By including the pineapple extract and the cinnamon extract at such a content ratio, the synergistic effect becomes more prominent, and a more excellent melanin production suppressing action and collagen production promoting action can be exhibited.

  The melanin production inhibitor or collagen production promoter of the present embodiment may be composed only of an extract from pineapple and an extract from cinnamon mushroom, or may be a mixture / formulation of these extracts.

  The melanin production inhibitor or collagen production promoter of this embodiment is a powder, granule, tablet, or the like according to a conventional method using a pharmaceutically acceptable carrier such as dextrin or cyclodextrin or any other auxiliary agent. It can be formulated into any dosage form such as liquid. In this case, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring / flavoring agent, and the like can be used. The melanin production inhibitor or collagen production promoter can be used by blending with other compositions (for example, external preparations for skin, foods and beverages for cosmetics, etc.), and also used as an ointment, a liquid for external use, a patch, etc. can do.

  When the melanin production inhibitor or collagen production promoter of the present embodiment is formulated, the total content of the extract from pineapple and the extract from cinnamon is not particularly limited, and is appropriately set according to the purpose. can do.

  In addition, the melanin production inhibitor or the collagen production promoter of the present embodiment, if necessary, extracts other natural extracts having a melanin production inhibitory action or a collagen production promotion action from extracts from pineapples and cinnamon moss. It can be used together with the extract as an active ingredient.

  Examples of the method of administration of the melanin production inhibitor or collagen production promoter of the present embodiment to a patient include transdermal administration, oral administration, and the like. Depending on the type of disease, a suitable method for its prevention / treatment, etc. What is necessary is just to select suitably.

  In addition, the dose of the melanin production inhibitor or collagen production promoter of the present embodiment may be appropriately increased or decreased depending on the disease type, severity, patient differences, administration method, administration period, and the like.

  The melanin production inhibitor of the present embodiment can prevent, treat, or improve spots, buckwheat, and the like through the melanin production inhibitory action of the extract from pineapple and the extract from betel grass. Moreover, the melanin production inhibitor of this embodiment can be used as an active ingredient of the pharmaceutical for prevention and treatment of the disease resulting from abnormal production of melanin, or a quasi-drug. However, the melanin production inhibitor of the present embodiment can be used for all purposes other than these uses, which are meaningful for exerting a melanin production inhibitory action.

  Moreover, the collagen production promoter of this embodiment can prevent or ameliorate skin aging symptoms such as wrinkles and sagging through the action of promoting type I collagen production of the extract from pineapple and the extract from cinnamon moss. . In addition, the collagen production promoter of the present embodiment prevents, treats or ameliorates diseases caused by decreased collagen production such as osteoporosis through the collagen production promoting action of the extract from pineapple and the extract from cinnamon moth; It can be used for the purpose of promoting the regeneration of damaged tendons and ligaments; promoting the healing of wounds or burns; However, the type I collagen production promoter of the present embodiment can be used for all purposes that are meaningful for exerting a type I collagen production promoting effect in addition to these uses.

  Moreover, since the melanin production inhibitor and collagen production promoter of this embodiment have the outstanding melanin production inhibitory effect and collagen production promotion effect, they are suitable for mix | blending with a skin external preparation, for example. In this case, the extract from pineapple and the extract from cinnamon can be blended as they are, respectively, these mixtures can be blended as they are, and further, the extract from pineapple and the extract from cinnamon are used. A blended and formulated melanin production inhibitor or collagen production promoter may be blended.

  Here, the external preparation for skin is not limited in its category, and includes a wide range of quasi-drugs and pharmaceuticals used transdermally, in addition to the skin cosmetics described below.

  Moreover, since the melanin production inhibitor and collagen production promoter of this embodiment have an excellent melanin production suppression action and collagen production promotion action, they are related to research related to melanin production mechanism and type I collagen production mechanism. It can be suitably used as a reagent for research.

[Skin cosmetic]
The extract from pineapple and the extract from cinnamon are suitable for blending into skin cosmetics because they have excellent melanin production inhibitory action and collagen production promoting action. In this case, the extract from pineapple and the extract from cinnamon can be blended as they are, respectively, these mixtures can be blended as they are, and further, the extract from pineapple and the extract from cinnamon are combined. -You may mix | blend the formulated melanin production inhibitor or collagen production promoter. By blending an extract from pineapple and an extract from betel grass, or a mixture thereof, the above melanin production inhibitor, or the above collagen production promoter into skin cosmetics, the skin cosmetics can suppress melanin production or produce collagen. A promoting action can be imparted.

  Skin cosmetics that can be blended with an extract from pineapple and an extract from cinnamon oyster, or a mixture thereof, the melanin production inhibitor, or the collagen production promoter are not particularly limited. , Creams, emulsions, lotions, packs, foundations, lip balms, bath salts, hair nicks, hair lotions, soaps, body shampoos and the like.

  When an extract from pineapple and an extract from cinnamon or a mixture thereof are blended in skin cosmetics, the blending amount can be adjusted as appropriate according to the type of skin cosmetic, but an extract from pineapples And the suitable compounding rate at the time of converting with the total amount of the extract from a cinnamon is about 0.0001-10 mass%, and a particularly suitable compounding rate is about 0.001-1 mass%.

  The skin cosmetic of the present embodiment is a main agent or auxiliary used in the production of normal skin cosmetics, as long as it does not interfere with the melanin production inhibiting action or collagen production promoting action of the extract from pineapple and the extract from quince Or other ingredients such as astringents, bactericides / antibacterial agents, whitening agents, UV absorbers, moisturizers, cell activators, anti-inflammatory / anti-allergic agents, antioxidant / active oxygen removers, fats and oils, waxes, carbonization Hydrogens, fatty acids, alcohols, esters, surfactants, fragrances and the like can be used in combination. When used in combination, it becomes a more general product, and a synergistic effect with other active ingredients used in combination may lead to an excellent effect that is more than normally expected.

  The skin cosmetic according to the present embodiment prevents, treats or improves spots, buckwheat, etc., through melanin production inhibiting action or collagen production promoting action; prevention / treatment of diseases caused by abnormal production of melanin; wrinkles, sagging skin, etc. To prevent or improve the aging symptoms of patients; prevention, treatment or improvement of diseases caused by decreased collagen production such as osteoporosis; promotion of regeneration of damaged tendons and ligaments; promotion of healing of wounds or burns; it can.

[Food and Drink]
Since the extract from pineapple and the extract from Buttercup have excellent melanin production inhibitory action and collagen production promoting action, they are suitable for blending into foods and drinks. In this case, in this case, the extract from the pineapple and the extract from the buttercup may be blended as they are, respectively, or the mixture thereof may be blended as it is, and further the extract from the pineapple and the extract from the buttercup A melanin production inhibitor or collagen production promoter containing a product may be blended.

  Here, food and drink are those that are less likely to harm human health and are taken by oral or gastrointestinal administration in normal social life. It is not limited to such categories. Therefore, the “food or drink” in this embodiment includes a wide variety of general foods, health foods, health functional foods, quasi drugs, pharmaceuticals, and the like that are taken orally.

  A melanin production inhibitor or a collagen production promoter that is formulated and formulated with an extract from pineapple and an extract from betel grass, or a mixture thereof, or an extract from pineapple and an extract from betel grass is added to food and drink. In this case, the amount of the active ingredient in them can be appropriately changed in consideration of the purpose of use, symptoms, sex, etc., but in consideration of the general intake of foods and drinks to be added, the adult day It is preferable that the amount of the perilla extract intake is about 1 to 1000 mg in total of the extract from pineapple and the extract from cinnamon. In addition, when the food and drink to be added is a granular, tablet or capsule food or drink, the addition amount of the melanin production inhibitor or the collagen production promoter is usually 0.1 to 100% by mass with respect to the food and drink to be added. Preferably, it is 5-100 mass%.

  The food / beverage products of this embodiment may be an extract from pineapple, an extract from cinnamon moss or a mixture thereof in any food / beverage product that does not impede its activity, or from pineapple. It may also be a dietary supplement based on an extract and an extract from a Snapper or a mixture thereof.

  When producing the food and drink of this embodiment, for example, sugars such as dextrin and starch; proteins such as gelatin, soybean protein and corn protein; amino acids such as alanine, glutamine and isoleucine; Arbitrary auxiliary agents, such as saccharides; oils and fats, such as soybean oil and medium chain fatty acid triglyceride, can be added, and it can be set as food / beverage products of arbitrary shapes.

  Foods and drinks that can be blended with an extract from pineapple and an extract from cinnamon or a mixture thereof are not particularly limited, and specific examples thereof include beverages such as soft drinks, carbonated drinks, nutrition drinks, fruit drinks, and lactic acid drinks. (Contains concentrated beverage concentrate and powder for adjustment); Ice cream, ice sherbet, shaved ice, and other frozen desserts; buckwheat noodles, udon, harsame, gyoza skin, sukimai skin, Chinese noodles, instant noodles and other noodles; , Chewing gum, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, etc .; fishery products such as kamaboko, ham, sausage; Products: salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing, etc. Fats and oils and processed foods; sauces, sauces and other seasonings; soups, stews, salads, prepared dishes, pickles; various other forms of health and nutritional supplements; tablets, capsules, drinks, etc. When blending an extract from pineapple and an extract from cinnamon moss or a mixture thereof into a product, auxiliary materials and additives that are usually used can be used in combination.

  In addition, although the melanin production inhibitor, collagen production promoter, skin cosmetics, and foods and beverages of this embodiment are suitably applied to humans, as long as the respective effects are exhibited, other than humans The present invention can also be applied to other animals (eg, mouse, rat, hamster, dog, cat, cow, pig, monkey, etc.).

  Hereinafter, although a test example is shown and this invention is demonstrated concretely, this invention is not restrict | limited to each following example at all. In the following test examples, pineapple fruit extract A (manufactured by Maruzen Pharmaceutical Co., Ltd., containing 20% ceramide, sample 1) or pineapple fruit extract B (manufactured by Maruzen Pharmaceutical Co., Ltd., containing 4% ceramide, sample 2) ), And Katsura flower extract (manufactured by Maruzen Pharmaceutical Co., Ltd., sample 3) was used as an extract from the cinnamon moss.

[Test Example 1] Test for inhibiting melanin production on B16 melanoma cells The extract (samples 1 and 3) was tested for melanin production inhibiting action on B16 melanoma cells as follows.

B16 melanoma cells were cultured using Dulbecco's MEM medium containing 10% FBS, and then cells were collected by trypsin treatment. The collected cells were diluted with Dulbecco's MEM medium containing 10% FBS and 1 mmol / L theophylline to a cell density of 24.0 × 10 ⁴ cells / mL, and then seeded at 300 μL per well in a 48-well plate. Incubate for hours.

  After completion of the culture, 10% FBS and 1 mmol / L theophylline-containing Dulbecco's MEM medium to which a test sample (sample 1 and / or sample 3, sample concentration is shown below) is added, or 10% FBS and 1 mmol / L without the sample are added. 300 μL of L theophylline-containing Dulbecco's MEM medium was added to each well and cultured for 4 days. After completion of the culture, the medium was removed from each well, 200 μL of 2 mol / L NaOH solution was added, the cells were disrupted with an ultrasonic disrupter, and the absorbance at a wavelength of 475 nm was measured to obtain the amount of melanin produced.

  In order to measure cell viability, the cells were cultured in the same manner as described above, then the medium was removed, washed with 400 μL of PBS buffer, and dissolved in Dulbecco MEM containing 10% FBS at a final concentration of 0.05 mg / mL. 200 μL of the neutral red was added to each well and cultured for 2.5 hours. After incubation, the neutral red solution was removed, and 200 μL of ethanol / acetic acid solution (ethanol: acetic acid: water = 50: 1: 49) was added to each well to extract the dye. After extraction, the absorbance at a wavelength of 540 nm was measured. From the obtained results, the melanin production inhibition rate (%) corrected by the cell viability was calculated by the following formula.

Melanin production inhibition rate (%) = {1− (B / D) / (A / C)} × 100
In the above formula, A represents “absorbance at 475 nm without addition of sample”, B represents “absorbance at 475 nm without addition of test sample”, C represents “absorbance at 540 nm without addition of sample”, D Represents “absorbance at 540 nm with addition of test sample”.
The results are shown in Table 1.

  As shown in Table 1, either sample 1 or sample 3 can independently suppress the production of melanin, but by processing sample 1 and sample 3 together, synergistic production of melanin is achieved. Suppressed. Therefore, it was recognized that a very excellent melanin production inhibitory effect was exhibited by using an extract from pineapple and an extract from Buttercup.

[Test Example 2] Type I collagen production promoting action test The extract (samples 2 and 3) was tested for type I collagen production promoting action as follows.

Human normal fibroblasts (NB1RGB) were cultured using Dulbecco's MEM medium containing 10% FBS, and then cells were collected by trypsin treatment. The collected cells were diluted with the above medium to a cell density of 1.6 × 10 ⁵ cells / mL, then seeded at 100 μL per well in a 96-well microplate and cultured overnight.

  After completion of the culture, the medium was removed and Dulbecco's MEM medium containing 0.25% FBS to which the test sample (sample 2 and / or sample 3, sample concentration is shown in Table 2 below) was added or 0.25% FBS without the sample added. 150 μL of Dulbecco's MEM medium was added to each well and cultured for 3 days. After culture, the amount of type I collagen in the medium of each well was measured by ELISA. From the measurement results, the type I collagen production promotion rate (%) was calculated by the following formula.

Type I collagen production promotion rate (%) = A / B × 100
In the above formula, A represents “amount of type I collagen with addition of test sample”, and B represents “amount of type I collagen without addition of sample”.
The results are shown in Table 2.

  As shown in Table 2, both Sample 2 and Sample 3 were treated together with Sample 2 and Sample 3 even though they were unable to promote type I collagen production alone. Promoted collagen production. Therefore, it was recognized that a combination of an extract from pineapple and an extract from cinnamon moss exhibited a very excellent type I collagen production promoting effect.

[Formulation Example 1]
A milky lotion was produced by a conventional method according to the following composition.
Pineapple fruit extract A 0.25g
Katsura flower extract 2.0g
Jojoba oil 4.0g
1,3-butylene glycol 3.0 g
Arbutin 3.0g
Polyoxyethylene cetyl ether (20E.O.) 2.5g
Olive oil 2.0g
Squalane 2.0g
Cetanol 2.0g
Glyceryl monostearate 2.0g
Oleic acid polyoxyethylene sorbitan (20E.O.) 2.0g
Methyl paraoxybenzoate 0.15g
Stearyl glycyrrhizinate 0.10g
Twilight extract 0.10g
Dipotassium glycyrrhizinate 0.10g
Ginkgo biloba extract 0.10g
Conchiolin 0.10g
Oat extract 0.10g
Chamomile extract 0.10g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

[Formulation Example 2]
A cream having the following composition was produced by a conventional method.
Pineapple fruit extract B 0.3g
Katsura Flower Extract 1.0g
Kujin extract 0.1g
Ogon Extract 0.1g
Liquid paraffin 5.0g
Salami beeswax 4.0g
Squalane 10.0g
Cetanol 3.0g
Lanolin 2.0g
Stearic acid 1.0g
Oleic acid polyoxyethylene sorbitan (20E.O.) 1.5g
3.0 g glyceryl monostearate
Oil soluble licorice extract 0.1g
1,3-butylene glycol 6.0 g
1.5 g of methyl paraoxybenzoate
Fragrance 0.1g
Purified water remainder (total amount is 100 g)

[Composition Example 3]
A serum having the following composition was produced by a conventional method.
Pineapple fruit extract A 0.1g
Katsura flower extract 2.0g
Chamomile extract 0.1g
Carrot extract 0.1g
Xanthan gum 0.3g
Hydroxyethylcellulose 0.1g
Carboxyvinyl polymer 0.1g
1,3-butylene glycol 4.0 g
0.1g dipotassium glycyrrhizinate
Glycerin 2.0g
Potassium hydroxide 0.25g
Fragrance 0.01g
Preservative (Methyl paraoxybenzoate) 0.15g
Ethanol 2.0g
Purified water remainder (total amount is 100 g)

[Formulation Example 4]
A tablet having the following composition was produced by a conventional method.
Pineapple fruit extract A 3.0mg
Katsura flower extract 12.5mg
Dolomite (containing 20% calcium and 10% magnesium) 78.4mg
Casein phosphopeptide 16.7mg
Vitamin C 33.4mg
Maltitol 131.3mg
Collagen 12.7mg
Sucrose fatty acid ester 12.0mg

[Formulation Example 5]
By an ordinary method, an oral liquid preparation having the following composition was produced.
<Composition in one ampoule (one 100 mL)>
Pineapple fruit extract A 0.5% by mass
Katsura Flower Extract 2.0% by mass
Sorbit 12.0% by mass
Sodium benzoate 0.1% by mass
Fragrance 1.0% by mass
Calcium sulfate 0.5% by mass
Purified water balance (100% by mass)

  The melanin production inhibitor of the present invention can greatly contribute to the prevention and improvement of skin pigmentation and the like. Moreover, the collagen production promoter of the present invention can greatly contribute to prevention or improvement of skin aging such as wrinkles and sagging.

Claims (3)

  A collagen production promoter comprising, as active ingredients, an extract from pineapple and an extract from betel grass. A skin cosmetic comprising the collagen production promoter according to claim 1 . A cosmetic food or drink comprising the collagen production promoter according to claim 1 .