JP5025201B2 - Moisturizer, anti-aging agent, whitening agent, anti-inflammatory agent, and antioxidant - Google Patents

Description

  The present invention relates to a humectant, an anti-aging agent, a whitening agent, an anti-inflammatory agent, and an antioxidant. More specifically, the present invention relates to a moisturizer, an anti-aging agent, a whitening agent, an anti-inflammatory agent, and an anti-oxidant containing as an active ingredient an extract of one or two or more kinds of plants selected from the plant of the genus Sagaribana or the genus Botanus.

  Factors of skin elasticity with age and aging symptoms such as wrinkles and spots include decreased cell function, decreased or denatured extracellular matrix components such as collagen, melanin production and pigmentation by UV rays, and oxidative damage to cells. It is done. In order to prevent and ameliorate such aging symptoms, various active ingredients have been searched for and studied for compounding. As a cell activator, the essence of Ponkan (see Patent Document 1), as a collagenase inhibitor, a dicarboxylic acid (see Patent Document 2), and as a collagen production promoter, an extract from a bud of a beech family, As a whitening agent, a water and / or organic solvent extract of white crane reishi (see Patent Document 4), and as an antioxidant, an extract of a plant belonging to the genus Sarogase (see Patent Document 5). Tea polyphenols (see Patent Document 6) are known as anti-inflammatory agents, and chlorella extracts (see Patent Document 7) are known as aromatase activity promoters.

JP 2001-131045 A Japanese Patent Laid-Open No. 9-124472 Japanese Patent Laid-Open No. 10-203952 JP 2003-89630 A Japanese Patent Laid-Open No. 10-182413 JP-A-6-9391 JP 2004-189609 A JP 2003-226613 A

  Naturally-derived components are known to have various pharmacological and cosmetic effects, and so far many plants and fungi have been widely applied to fields such as external preparations for skin and foods and drinks. However, there are many naturally-derived ingredients whose effects are not yet known, and the development of active ingredients with excellent moisturizing, anti-aging, anti-inflammatory, or antioxidant effects is expected. It was. The present invention has been made in order to find such an active ingredient, and is a moisturizer, anti-aging agent, whitening agent, anti-inflammatory agent, and anti-inflammatory agent that can be widely applied in the fields of external preparations for skin and foods and drinks. It is an object to provide an oxidizing agent.

  In order to solve the above problems, the present inventors have studied various naturally-derived substances with respect to a moisturizing action, an anti-aging action, a whitening action, an anti-inflammatory action, and an antioxidant action. As a result, we found excellent moisturizing action, anti-aging action, whitening action, anti-inflammatory action, and anti-oxidation action for one or more plant extracts selected from the genus Sagaribana or Botanus. As a result, the present invention was completed.

  That is, the present invention relates to a moisturizing agent, an anti-aging agent, a whitening agent, an anti-inflammatory agent, and an anti-oxidant comprising as an active ingredient an extract of one or two or more kinds of plants selected from the plant of the genus Sagaribana or the genus Botanus. About.

  According to the present invention, it is possible to provide a moisturizer, an anti-aging agent, a whitening agent, an anti-inflammatory agent, and an antioxidant that have excellent effects. In addition, by blending these into topical skin preparations and foods, a composition that exhibits excellent effects in preventing and improving various skin symptoms such as wrinkles, tarmi, reduction of skin firmness, spots, wrinkles, fine wrinkles, and dryness. Things can be provided.

Plants used as a raw material of the present invention may be any plant of lecythidaceae (Lecythidaceae) barringtonia genus (Barringtonia) or Hougan'noki genus (Couroupita). As plants belonging to the genus Sagaribana, sagaribana ( Barringtonia racemosa ), barn reed ( Barringtonia asiatica ) and the like are known. The Hougan'noki plants of the genus, such as Hougan'noki (Couroupita guianensis) is known.

The plant used as a raw material for the present invention is not particularly limited as long as it is a plant belonging to the genus Sagaribana or the genus Boletus, but for reasons such as being relatively easy to obtain and effective, it is preferable to use Barringtonia asiatica. This is particularly preferable from the viewpoint of effectiveness.

  In the present invention, the extract of the genus Sagaribana or the genus Botanus includes the original or dried product of the plant of the genus Sagari or genus, but it is possible to use extracts extracted using various solvents. preferable. For extraction, any part of the leaves, flowers, seeds, roots, bark, buds, etc. may be used for extraction, but leaves, stems, and seeds are used for convenient use. It is better to use seeds from the viewpoint of effectiveness. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days.

  Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as ethyl ether and propyl ether. And solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  Extracts from the above-mentioned solvents of the genus Sagaribana or Botanus can be used as they are, but the concentrated and dried solids can be used by re-dissolving them in water or a polar solvent. It may be used after performing a purification treatment such as decolorization, deodorization, and desalting, and a fractionation treatment by column chromatography or the like within a range not impairing the above. The said extract of Sagaribana genus plant or Botanus genus plant, its processed material, and the fractionation thing can be freeze-dried after each processing and fractionation, and can also be melt | dissolved and used at the time of use.

  A moisturizing agent containing as an active ingredient an extract of the genus Sagaribana or Botanus spp. Exhibits an excellent moisturizing effect on the skin and hair, and in particular has a high moisturizing effect on the skin.

  An anti-aging agent comprising an extract of the genus Sagaribana or Botanus spp. As an active ingredient has an excellent cell activation effect, collagen production action, and aromatase activity promotion action, and exhibits an excellent effect in preventing and improving aging symptoms To do. Aromatase is an enzyme that works when producing estrogen. If the production of estrogen is promoted by an aromatase activity promoting action, a skin beautifying effect and an anti-aging effect by female hormones can be expected.

  A whitening agent containing an extract of the genus Sagaribana or Botanus genus as an active ingredient is effective in improving pigmentation symptoms such as spots and buckwheat, and is particularly effective for inhibiting tyrosinase activity and suppressing melanin production. Demonstrate.

  An anti-inflammatory agent containing an extract of the genus Sagaribana or Botanus as an active ingredient has an excellent hyaluronidase inhibitory effect, suppresses skin inflammation, and exhibits an excellent anti-inflammatory action.

  Antioxidants containing extracts of the genus Sagaribana or Botanus spp. As active ingredients have excellent free radical scavenging effects and superoxide anion scavenging effects, and prevent skin photoaging etc. Demonstrate antioxidant effect.

  The extract of the genus Sagaribana or the genus Botanus has an excellent moisturizing action, anti-aging effect, whitening action, anti-inflammatory action, and anti-oxidation action, moisturizing agent, anti-aging agent, whitening agent, anti-inflammatory agent, And can be used as an antioxidant. In addition, moisturizers, anti-aging agents, whitening agents, anti-inflammatory agents, and antioxidants that contain extracts of the genus Sagaribana or Botanus genus plant as active ingredients are not only externally applied to the skin, but also to the hair. It can be used or taken orally, and can be widely applied to foods, beverages, pharmaceuticals, and the like.

  The amount of sagaribana plant or genus genus plant extract added to skin preparations and foods can be adjusted depending on the type of skin preparations and foods, purpose of use, etc. From the point, 0.0001 to 50.0% by weight is preferable with respect to the total amount, and more preferably 0.001 to 20.0% by weight.

  The dosage form of the external preparation for skin containing the extract of the genus Sagaribana or the genus Botanus is arbitrary. For example, it is provided as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. be able to. Further, it can be provided in various dosage forms such as aerosol, lipstick, and foundation filled with a propellant.

  In addition, the external preparation for skin blended with the extract is usually blended with pharmaceuticals, quasi-drugs, skin cosmetics, hair cosmetics and cleansing agents as needed, in addition to these extracts. Oily ingredients, moisturizers, powders, pigments, emulsifiers, solubilizers, detergents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial / antifungal agents, alcohols, etc. can be added as appropriate. . In addition, other moisturizers, anti-aging agents, whitening agents, anti-inflammatory agents, or antioxidants can be used in combination as long as the effects of the present invention are not impaired.

  Moreover, the dosage form of the food containing the extract of the genus Sagaribana or the genus Botanus is arbitrary, but it can be provided in various dosage forms such as powders, granules, capsules, liquids, etc. The oily ingredients, moisturizers, powders, emulsifiers, solubilizers, thickeners, drugs, fragrances, antibacterial and antifungal agents, alcohols, sugar , Condensed milk, flour, salt, glucose, chicken egg, butter, margarine, starch syrup, calcium, iron, seasoning, spice, vitamin A and derivatives thereof, carotenoids, riboflavin and derivatives thereof, vitamin B and salts or derivatives thereof , Ascorbic acid and its derivatives, cobalamins, vitamin E and their derivatives, vitamin K, adenosine and its derivatives, flavonoids and tannins It is also possible to focus. Furthermore, it can be used in combination with other moisturizers, anti-aging agents, whitening agents, anti-inflammatory agents, or antioxidants as long as the effects of the present invention are not impaired.

  The production examples of the extracts of the genus Sagaribana or the genus Botanus, the tests for evaluating each action, the formulation examples as external preparations for skin and foods, and the use tests will be described in detail below. The range is not limited by this.

[Extraction method 1]
The genus Sagaribana or the genus Botanus was dried and pulverized, added with 20% by mass of 50% by mass of ethanol and extracted for 2 hours while stirring at room temperature. The obtained extract was filtered to remove insolubles, concentrated under reduced pressure, and then freeze-dried to obtain an extract.

[Extraction method 2]
The genus Sagaribana or the genus Botanus was dried and pulverized, purified water having an amount 20 times the mass of the sample was added, and the mixture was extracted by heating to 120 ° C. for 20 minutes using an autoclave. From the obtained extract, insoluble matters were removed by suction filtration while maintaining a high temperature state, and then lyophilized to obtain an extract.

<Evaluation of dermal fibroblast activation effect>
Using the ethanol extract obtained by the extraction method 1 as a sample, the dermal fibroblast activation action was evaluated as follows.

Normal human dermal fibroblasts manufactured by Kurashiki Boseki Co., Ltd. were seeded in a 96-well microplate so that there were 2.0 × 10 ⁴ cells per well. As the seeding medium, Dulbecco's modified Eagle medium (DMEM) to which 1% by mass of fetal bovine serum (FBS) was added was used. After culturing for 24 hours, the medium was replaced with a sample culture solution adjusted to each sample concentration shown in Table 1 with 1% by mass FBS-added DMEM medium, and further cultured for 48 hours. After removing the supernatant, the medium was replaced with a medium containing 400 μg / ml of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT reagent) and cultured for about 2 hours. Thereafter, formazan produced by the opening of the tetrazolium ring was extracted with 2-propanol, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values.

  The obtained results are shown in Table 1 in terms of relative values when the cell activation effect in the sample-free control is taken as 100.

  As is clear from Table 1, a significant dermal fibroblast activation effect was observed in the medium to which the sample was added.

<Evaluation of epidermal cell activation effect>
Using the ethanol extract obtained by the extraction method 1 as a sample, the epidermal cell activation action was evaluated as follows.

Human epidermal non-keratinized cells were seeded in a 96-well microplate so that there were 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS). After culturing for 24 hours, the culture medium was replaced with a sample culture solution adjusted to each sample concentration shown in Table 2 with 5% by mass FBS-added DMEM medium, and further cultured for 24 hours. After removing the supernatant, the medium was replaced with a medium containing 100 μg / ml of MTT reagent and cultured for about 2 hours. Thereafter, formazan produced by the opening of the tetrazolium ring was extracted with 2-propanol, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values.

  The obtained results are shown in Table 2 by relative values when the cell activation effect in the control with no sample added is defined as 100.

  As is clear from Table 2, a significant epidermal cell activation effect was observed in the medium to which the sample was added.

<Evaluation of dermal fibroblast collagen production>
Using the ethanol extract and hot water extract obtained by the extraction methods 1 and 2 as samples, the dermal fibroblast collagen production action was evaluated as follows.

Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS). After culturing for 24 hours, the medium was replaced with a culture solution adjusted to the sample concentration shown in Table 3 with 0.5% by mass FBS-added DMEM medium, and further cultured for 24 hours.

  The ELISA method was used for the quantification of type I and type IV collagen secreted into the culture supernatant, and finally 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) disulfide for labeled peroxidase. After adding ammonium salt (ABTS) and hydrogen peroxide for reaction, absorbance at 405 nm was measured with a microplate reader.

  The amount of protein was measured by BCA Protein Assay Kit manufactured by PIERCE, and the amount of type I and type IV collagen produced per unit protein was determined.

  The obtained results are shown in Table 3 by relative values when the amount of collagen production of type I type I and type cocoon per unit protein amount in the control with no sample added is defined as 100.

  As is clear from Table 3, in the medium to which the sample was added, a significant dermal fibroblast collagen production effect was observed.

<Evaluation of epidermal cell collagen production>
Using the ethanol extract obtained by the extraction method 1 as a sample, the epidermal cell collagen producing action was evaluated as follows.

Human epidermal non-keratinized cells were seeded in a 96-well microplate so that there were 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS). After culturing for 24 hours, the culture medium was replaced with a sample culture solution adjusted to each sample concentration shown in Table 4 with 5% by mass FBS-added DMEM medium, and further cultured for 5 days.

  For the quantification of type IV collagen secreted in the culture supernatant, avidinized horseradish peroxidase was added using a sandwich ELISA method using a monoclonal antibody against IV collagen (recognition site: α2 chain) and a biotinylated polyclonal antibody. The color was developed with 3,3 ′, 5,5′-tetramethylbenzidine, and the absorbance at 650 nm was measured with a microplate reader.

  The amount of protein was measured by BCA Protein Assay Kit manufactured by PIERCE, and the amount of type IV collagen produced per unit protein was determined.

  The obtained results are shown in Table 4 by relative values when the type IV collagen production amount per unit protein amount in the control with no sample added is defined as 100.

  As is clear from Table 4, in the medium to which the sample was added, a significant epidermal cell collagen production effect was observed.

<Evaluation of aromatase activity promoting action>
Using the ethanol extract obtained by the extraction method 1 as a sample, the aromatase activity promoting action was evaluated as follows.

In 4 μl of the sample solution prepared at each sample concentration shown in Table 5, a mixed solution of NADP +, MgCl ₂ , glucose-6-phosphate, glucose-6-phosphate dehydrogenase, and control insect cell membrane protein (CPY19 / MFC high-throughput inhibitor 96 μl of a screening kit (High Throughput Inhibitor Screening Kit), manufactured by BD Biosciences) was added and heated to 37 ° C. for 10 minutes. 100 μl of 15 nM CPY19 (aromatase), 50 μM 7-methoxy-4-trifluoromethylcoumarin (substrate) solution was added and warmed to 37 ° C. for 30 minutes. The reaction was stopped by adding 75 μl of 100 mM Tris base.

  Fluorescence measurement was performed at an excitation wavelength of 409 nm and an emission wavelength of 530 nm. Since 7-methoxy-4-trifluoromethylcoumarin is decomposed by CYP19 and 7-hydroxy-4-trifluoromethylcoumarin is generated to produce fluorescence, the aromatase activity promoting ability was quantified by fluorescence measurement.

  The obtained results are shown in Table 5 as relative values when the aromatase activity promoting action in the control without addition of the sample is taken as 100.

  As is apparent from Table 5, when the sample was added, a significant aromatase activity promoting action was observed.

<Evaluation of epidermal melanocyte tyrosinase activity inhibitory action>
Using the ethanol extract obtained by the extraction method 1 as a sample, the epidermal melanocyte tyrosinase activity inhibitory action was evaluated as follows.

Normal human epidermal melanocytes were seeded in a 96-well microplate so that there were 3.0 × 10 ⁴ cells per well. As a seeding medium, Medium 154S manufactured by Kurashiki Boseki Co., Ltd. was used. After culturing for 24 hours, the medium was replaced with a sample culture solution adjusted to each sample concentration shown in Table 6 using Medium 154S, and further cultured for 48 hours. Next, the cells were completely lysed by exchanging with 75 μl of a phosphate buffer containing 1% by weight Triton-X, and 50 μl of this was used as a crude enzyme solution. To the crude enzyme solution, 50 μl of 0.05% by mass L-dopa-containing phosphate buffer as a substrate was added and allowed to stand at 37 ° C. for 2 hours. The absorbance at 405 nm immediately after the addition of the substrate and at the end of the reaction was measured with a microplate reader, and each measured value was introduced into the following equation to determine the amount of produced dopamelanin.

Dopamelanin production =
{(405 nm value after reaction−405 nm value before reaction) −2.166} /5.238
Moreover, the amount of protein was measured by BCA Protein Assay Kit made by PIERCE, and the amount of melanin produced per unit protein amount was determined. Based on the amount of melanin produced, the inhibition rate with respect to the case where no sample was added was calculated.

  As is clear from Table 6, in the medium to which the sample was added, a decrease in melanin production was observed, and an excellent tyrosinase activity inhibitory effect was observed.

<Evaluation of hyaluronidase inhibitory action>
Using the ethanol extract obtained by the extraction method 1 as a sample, the hyaluronidase inhibitory action was evaluated as follows.

  Commercially available hyaluronic acid potassium salt (derived from human umbilical cord) was dissolved in 0.1 M phosphate buffer (pH 7.0) to a concentration of 0.9 mg / ml to obtain a substrate solution. A commercially available hyaluronidase (derived from bovine testis) was dissolved in 0.1 M phosphate buffer (pH 7.0) so as to be 5,300 units / ml to obtain an enzyme solution. The enzyme solution was prepared at the time of use.

  0.1 ml of the solution prepared to each sample concentration shown in Table 7 with buffer and 0.03 ml of enzyme solution were put in a test tube and reacted at 37 ° C. for 20 minutes. Next, 0.06 ml of activator was added and reacted at 37 ° C. for 20 minutes. Further, 0.15 ml of the substrate solution was added and reacted at 37 ° C. for 1 hour. The reaction was stopped by adding 0.06 ml of 0.4N NaOH aqueous solution, immediately cooled on ice, 0.06 ml of borate buffer (pH 9.1) was added and boiled for 3 minutes. did. After 2.0 ml of p-DABA (p-dimethylaminobenzaldehyde) solution was added and reacted at 37 ° C. for 20 minutes, the solution was transferred from each test tube to a 96-well microplate, and the absorbance at 585 nm was measured using a microplate reader. It was measured. As a control, a sample-free buffer solution was used. When the activity of hyaluronidase is inhibited, the degradation product N-acetylglucosamine (GlcNAc) is decreased, and the absorbance by p-DABA is lowered. The hyaluronidase inhibitory action is defined by the following formula.

Inhibition rate (%)
= (Control absorbance-sample absorbance) / control absorbance x 100
The results are shown in Table 7.

  As is apparent from Table 7, when the sample was added, an excellent inhibitory effect on hyaluronidase activity was observed.

<Evaluation of antioxidant effect by scavenging DPPH radical>
Using the extract obtained by the extraction method 1 or the extraction method 2 as a sample, the antioxidant action by DPPH radical elimination was evaluated as follows.

  Using 50% by mass ethanol, the sample solution was adjusted so as to have each sample concentration shown in Table 8, and 100 μl was added to each 96-well microplate. Thereto was added 100 μl of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) ethanol solution, mixed well, and allowed to stand at room temperature in the dark for 24 hours. Finally, the absorbance at 516 nm derived from the DPPH radical was measured.

  When the absorbance when the sample was not added was (A) and the absorbance when the sample was added was (B), the DPPH radical elimination rate was determined from the following equation.

Radical scavenging rate = {1- (B) / (A)} × 100
The results are shown in Table 8.

  As is apparent from Table 8, when the sample was added, an excellent DPPH radical scavenging effect was observed.

<SOD-like activity evaluation (evaluation of superoxide anion scavenging ability)>
Using the hot water extract obtained by the extraction method 2 as a sample, SOD-like activity was evaluated as follows.

  To 75 μl of the HANK ′S (+) solution containing 0.25 mM WST-1 and 1 mM Hypoxanthine, 25 μl of the sample solution prepared by the HANK ′S (+) solution to each sample concentration shown in Table 9 was added. Further, 25 μl (0.0075 Units) of xanthine oxidase was added and reacted at 37 ° C. for 15 minutes, and then the absorbance at 450 nm was measured. The superoxide anion elimination rate is defined by the following equation, where (A) is the absorbance when only the HANK'S (+) solution is added instead of the sample solution, and (B) is the absorbance when the sample solution is added. Is done.

Erase rate (%) = {1- (B) / (A)} × 100
Table 9 shows the obtained results.

  As is clear from Table 9, when the sample was added, an excellent superoxide anion erasing effect was observed.

  Then, the formulation example of a skin external preparation and a foodstuff is shown as a composition which mix | blended the extract of the genus Sagaribana or the genus Botanus concerning the present invention.

[Formulation Example 1] Emulsion (1) Squalane 10.0 (wt%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 53.85
(11) Arginine (1 wt% aqueous solution) 20.0
(12) Cobannoashi extract (leaves) [Extraction method 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.

[Prescription Example 2] Lotion (1) Ethanol 15.0 (% by weight)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 78.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Cobannoashi extract (leaves) [Extraction method 1] 5.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Prescription Example 3] Cream (1) Squalane 10.0 (% by weight)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20% by weight aqueous solution) 15.0
(10) Purified water 36.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Cobannoashi extract (leaves) [Extraction method 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.

[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (% by weight)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1 wt% aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by weight aqueous solution) 2.0
(16) Cobannoashi extract (leaves) [Extraction method 1] 5.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.

[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (% by weight)
(2) Purified water 78.7
(3) Sodium hydroxide (10% by weight aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Cobannoashi extract (leaves) [Extraction method 1] 10.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Formulation Example 6] Cleansing Fee (1) Squalane 77.0 (wt%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 3.0
(4) Cobannoashi extract (leaves) [Extraction method 1] 5.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Prescription Example 7] Face-wash foam (1) Stearic acid 16.0 (% by weight)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 31.5
(8) Cobannoashi extract (leaves) [Extraction method 1] 6.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Prescription Example 8] Make-up base cream (1) Squalane 10.2 (% by weight)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 65.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Cobannoashi extract (leaves) [Extraction method 1] 5.0
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Formulation Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (wt%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 53.4
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Cobannoashi extract (leaves) [Extraction method 1] 5.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Formulation Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Cobannoashi extract (leaves) [Extraction method 1] 5.0
(11) Purified water 43.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

[Prescription Example 11] Pack (1) Purified water 58.9 (% by weight)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Cobannoashi extract (leaves) [Extraction method 1] 5.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

[Prescription Example 12] Bath agent (1) Fragrance 0.3 (% by weight)
(2) Cobannoashi extract (leaves) [Extraction method 1] 5.0
(3) Sodium bicarbonate 46.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.

[Prescription Example 13] Hair wax (1) Stearic acid 3.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 70.6
(11) Cobannoashi extract (leaves) [Extraction method 2] 5.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 14] Hair artic (1) Ethanol 46.0 (% by weight)
(2) Purified water 48.9
(3) Cobannoashi extract (leaves) [Extraction method 1] 5.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

[Prescription Example 15] Beverage (1) Cobannoashi Extract (Leaf) [Extraction Method 1] 8.0 (wt%)
(2) Erythritol 1.0
(3) Citric acid 0.1
(4) Stevia 0.01
(5) Purified water 90.89
Production method: (1) to (5) are mixed uniformly.

[Prescription Example 16] Tablet (1) Cobannoashi extract (leaves) [Extraction method 2] 0.30 (parts by weight)
(2) Reduced maltose starch syrup 0.53
(3) Corn starch 0.15
(4) Glycerin fatty acid ester 0.02
Production method: (1) to (3) were sieved and mixed, and (4) was further added and mixed. Tableting was performed with a tableting machine to obtain tablets with a total amount of 300 mg.

  Next, a use test was conducted using a prescription blended with an extract of a genus Sagaribana or a genus plant, and the effect of improving skin roughness due to drying was evaluated. At that time, the samples shown in Table 10 were blended with the emulsion formulation shown in Formulation Example 1, and the use test was conducted as Examples 1 to 3. Further, the sample was replaced with purified water, and a use test was conducted simultaneously as Comparative Example 1.

  For each sample, 20 female panelists with dry skin in their 30s to 50s whose skin symptom was remarkably recognized were grouped and used blindly for 1 week, and the skin condition before and after use was observed and evaluated. . As an index of skin symptom, rough skin due to dryness was evaluated in three stages of “improved”, “slightly improved”, and “no change”, and Table 11 shows the number of panelists that obtained each evaluation.

  From Table 11, in the Example use group which mix | blended the sample, clear improvement of rough skin was recognized. From this, it has been clarified that the extract of the genus Sagaribana or the genus Botanus has an excellent moisturizing effect.

Claims (2)

A moisturizing agent comprising as an active ingredient an extract of one or more plants selected from a plant belonging to the genus Sagaribana or a genus Botanus. The antioxidant which uses the extract of the 1 type, or 2 or more types of plant chosen from Sagaribana genus plant or Botanus genus plant as an active ingredient.