JP4201091B1 - Moisturizer, anti-aging agent, antioxidant and external preparation for skin - Google Patents

Abstract

The present invention provides a moisturizer, an anti-aging agent, and an antioxidant that can be widely applied in the field of external preparations for skin and foods and drinks.
[MEANS FOR SOLVING PROBLEMS] A moisturizing agent, an anti-aging agent, an antioxidant and an external preparation for skin, each comprising an extract of one or more plants selected from plants of the genus Genus.
[Selection figure] None

Description

  The present invention relates to a humectant, an anti-aging agent, an antioxidant and a skin external preparation. More specifically, the present invention relates to a moisturizer, an anti-aging agent, an antioxidant and an external preparation for skin, each of which contains an extract of one or two or more plants selected from the genus Zazensou.

  Causes of aging symptoms such as skin elasticity reduction and wrinkles with aging include decreased cell function, decreased or degenerated extracellular matrix components such as collagen, and oxidative damage of cells. In order to prevent and ameliorate such aging symptoms, various active ingredients have been searched and compounded in the past. As a cell activator, the essence of Ponkan (see Patent Document 1), as a collagenase inhibitor, as dicarboxylic acid (see Patent Document 2), as a collagen production promoter, an extract from a bud of a beech family, As an antioxidant, an extract of a plant belonging to the genus Salogaceae (see Patent Document 4) is known. The use of genus genus plants for skin external preparations has not been known so far.

JP 2001-131045 A JP-A-9-124472 Japanese Patent Laid-Open No. 10-203952 JP 2003-89630 A JP-A-6-9391

  Naturally-derived components are known to have various pharmacological and cosmetic effects, and so far many plants and fungi have been widely applied to fields such as external preparations for skin and foods and drinks. However, there are many naturally-derived ingredients whose effects are not yet known, and the development of an effective ingredient having an excellent moisturizing action, anti-aging action or antioxidant action has been expected. The present invention has been made in order to find such an active ingredient, and it is an object to provide a moisturizer, an anti-aging agent, and an antioxidant that can be widely applied to fields such as external preparations for skin and foods and drinks. And

  In order to solve the above-mentioned problems, the present inventors have studied various naturally-derived substances with respect to moisturizing action, anti-aging action, and antioxidant action. As a result, an excellent moisturizing action, anti-aging action, and antioxidant action were found in the extract of one or more plants selected from the genus Genus, and further studies were made to complete the present invention.

  That is, the present invention relates to a moisturizing agent, an anti-aging agent, an antioxidant and an external preparation for skin, each of which contains an extract of one or more plants selected from the genus Genus.

  According to the present invention, it is possible to provide a moisturizer, an anti-aging agent, and an antioxidant having excellent effects. In addition, by blending these with topical skin preparations and foods, a composition that exhibits an excellent effect in preventing and improving various skin symptoms such as wrinkles, tarmi, reduction of skin firmness, wrinkles, and dryness is provided. be able to.

The plant used as a raw material of the present invention may be a plant of the genus Araceae and Symplocarp . The skunk cabbage plants of the genus, skunk cabbage (Symplocarpus foetidus Nutt.var. Latissimus (Makino ) H.Hara), such as Himezazensou (Symplocarpus nipponicus Makino) is known.

Plants as a raw material used in the present invention is not particularly limited as long as skunk cabbage plant of the genus, from reasons such as availability relative ease and effectiveness, skunk cabbage (Symplocarpus foetidus Nutt.var. Latissimus (Makino ) H .Hara) is preferably used.

  The extract of the genus genus plant in the present invention includes the active substance and dried product of the genus genus plant, but it is preferable to use an extract extracted with various solvents. For extraction, any part of the leaves of genus genus plant, Buddha buds, flowers, seeds, roots, stems, buds, etc. may be used. From the viewpoint of the effectiveness of the present invention, flowers, Buddha buds are used. It is good to use the ground part of the flowering period containing In the extraction, the raw material may be used as it is, but considering the extraction effect, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction effect, homogenization may be performed in stirring or an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days.

  Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. And solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia. Among these extraction solvents, it is preferable to use one or two selected from water and ethanol from the viewpoint of the effect of the present invention.

  Extracts from the above-mentioned genus genus plants can be used as they are, but concentrated and dried solids can be used by re-dissolving them in water or polar solvents, and their physiological effects are not impaired. It may be used after performing purification treatment such as decolorization, deodorization, and desalting, and fractionation treatment by column chromatography. The said extract of a genus genus genus, its processed material, and a fraction can also be used after freeze-drying after each process and fractionation, and melt | dissolving at the time of use.

  A moisturizing agent comprising an extract of a genus genus plant as an active ingredient exhibits an excellent moisturizing action on the skin and hair, and has a particularly high moisturizing effect on the skin.

  An anti-aging agent comprising an extract of a genus genus plant as an active ingredient has an excellent hyaluronic acid production promoting action, and exhibits an excellent effect in preventing and improving aging symptoms.

  Antioxidants containing extracts of genus genus plant as an active ingredient have excellent free radical scavenging effect and superoxide anion scavenging effect, prevent photo-aging of skin, etc., and have excellent antioxidant action Demonstrate.

  The extract of the genus genus plant has excellent moisturizing action, anti-aging action, and antioxidant action, and can be used as a moisturizing agent, anti-aging agent, antioxidant, and skin external preparation. In addition, moisturizers, anti-aging agents, and antioxidants containing extracts of the genus genus genus as active ingredients can be used not only for external use on the skin, but also for hair and can be taken orally. It can be widely applied to food, beverages, pharmaceuticals, etc.

  The amount of the extract of the genus genus plant can be adjusted depending on the type of skin external preparation and food and the purpose of use, etc. The content is preferably 0.0001 to 50.0% by mass, more preferably 0.001 to 20.0% by mass.

  The dosage form of the external preparation for skin containing the extract of the genus genus genus is arbitrary. For example, it can be provided as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. Further, it can be provided in various dosage forms such as aerosol, lipstick, and foundation filled with a propellant.

  In addition, the external preparation for skin blended with the above extract is usually blended with pharmaceuticals, quasi-drugs, skin cosmetics, hair cosmetics, and cleansing agents as needed in addition to these extracts. Oily ingredients, moisturizers, powders, pigments, emulsifiers, solubilizers, detergents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial / antifungal agents, alcohols, etc. can be added as appropriate. . In addition, other moisturizers, anti-aging agents, whitening agents, anti-inflammatory agents, or antioxidants can be used in combination as long as the effects of the present invention are not impaired.

  Moreover, the dosage form of the food containing the extract of the genus genus genus is arbitrary, but it can be provided in various dosage forms such as powders, granules, capsules, liquids, etc.・ Oil ingredients, moisturizers, powders, emulsifiers, solubilizers, thickeners, drugs, fragrances, antibacterial and antifungal agents, alcohols, sugar, condensed milk, wheat flour Salt, glucose, chicken eggs, butter, margarine, starch syrup, calcium, iron, seasonings, spices, vitamin A and derivatives thereof, carotenoids, riboflavin and derivatives thereof, vitamin B and salts or derivatives thereof, ascorbic acid and Derivatives thereof, cobalamins, vitamin E and derivatives thereof, vitamin K, adenosine and derivatives thereof, flavonoids and tannins can also be blended. Furthermore, it can be used in combination with other moisturizers, anti-aging agents, whitening agents, anti-inflammatory agents, or antioxidants as long as the effects of the present invention are not impaired.

  In the following, production examples of extracts of genus genus genus plants, tests for evaluating each action, formulation examples as external preparations for skin and foods, and use tests will be described in detail, but the technical scope of the present invention is based on this. It is not limited at all.

[Production Method 1]
The ground part of the flowering season of sensou was dried and pulverized, and a 50 mass% aqueous ethanol solution 20 times the mass of the sample was added, followed by extraction with stirring at room temperature for 2 hours. The obtained extract was filtered to remove insolubles, concentrated under reduced pressure, and then freeze-dried to obtain extract 1.

[Production Method 2]
The ground part of the flowering season of zenithou was dried and pulverized, and purified water in an amount 20 times the mass of the sample was added, followed by heating to 120 ° C. for 20 minutes for extraction. From the obtained extract, insolubles were removed by suction filtration, and then lyophilized to obtain Extract 2.

<Evaluation of moisturizing effect>
24 μL of the samples shown in Table 1 (Examples and Comparative Examples) were applied to the forearm, 3 × 4 cm ² range, and the keratin moisture content was measured before application, 15, 30, 60, and 120 minutes after application. The keratin water content was measured by using SKICON-200 (manufactured by IBI S Co., Ltd.) and measuring the keratin water content by 5 points from each application site. Table 2 shows the relative values with the average value of the five-point measurement values as the keratin water content and the keratin water content before application as 1. A significant difference test was performed between the comparative example and the example, and “*” indicates a significant difference with a probability of 5%, and “**” indicates a significant difference with a probability of 1%. It was attached to 2.

  As is apparent from Table 2, when the sensation extract was added, an excellent moisturizing effect was observed.

<Evaluation of antioxidant effect by scavenging DPPH radical>
The extract 1 solution was adjusted to a sample concentration shown in Table 3 using a 50% by mass aqueous ethanol solution, and 100 μL was added to each 96-well microplate. Thereto, 100 μL each of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) ethanol solution was added, mixed well, and allowed to stand in the dark at room temperature for 24 hours. Finally, the absorbance at 516 nm derived from the DPPH radical was measured. The extinction rate of the DPPH radical was calculated from the following equation, where (A) was the absorbance when no extract 1 was added and (B) was the absorbance when extract 1 was added. Radical scavenging rate = {1- (B) / (A)} × 100 Table 3 shows the results.

  As is apparent from Table 3, when an extract of the genus Pachyderma was added, an excellent DPPH radical scavenging effect was observed.

<SOD-like activity evaluation (superoxide anion scavenging ability evaluation)>
A HANK'S (+) solution (75 μL) containing 0.25 mM tetrazolium salt (WST-1) and 1 mM hypoxanthine was prepared with each sample concentration shown in Table 4 using the HANK'S (+) solution. 25 μL of Extract 2 solution was added. Furthermore, xanthine oxidase (Xanthine Oxidase) 25 microliters (0.0075Units) was added, and after making it react at 37 degreeC for 15 minute (s), the light absorbency of 450 nm was measured. Superoxide anion when (A) is the absorbance when only the HANK'S (+) solution is added instead of the solution of Extract 2 and (B) is the absorbance when the solution of Extract 2 is added The erasure rate is defined by the following equation. Erase rate (%) = {1- (B) / (A)} × 100 Table 4 shows the results obtained.

  As is apparent from Table 4, when an extract of the genus Pachyderma was added, an excellent superoxide anion scavenging effect was observed.

<Evaluation of human dermal fibroblast hyaluronic acid production action>
Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS). After 24 hours, the medium was replaced with a sample culture solution adjusted to each concentration in a DMEM medium supplemented with 0.5% FBS, and further cultured for 5 days. The indirect ELISA method using proteoglycan was used for the determination of hyaluronic acid secreted into the culture supernatant. As an indirect ELISA method, a 96-well microplate was coated with hyaluronic acid, an anti-keratan sulfate solution was used as a primary antibody, and an anti-mouse IgG antibody was used as a secondary antibody. Finally, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and hydrogen peroxide were added to each peroxidase and allowed to react with each well. Absorbance was measured at 405 nm. The amount of protein in each well was measured with PIERCE BCA Protein Assay Kit to determine the amount of hyaluronic acid produced per unit protein. The results obtained are shown in Table 5.

  As is apparent from Table 5, when an extract of the genus Pachyderma was added, an excellent hyaluronic acid production promoting effect was observed.

  Then, the formulation example of a skin external preparation and a foodstuff is shown as a composition which mix | blended the extract of the genus Genus according to this invention.

[Formulation Example 1] Emulsion (1) Squalane 10.0 (mass%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 53.85
(11) Arginine (1% by weight aqueous solution) 20.0
(12) Extract 1 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification, cooling is started, and (11) and (12) are sequentially added and mixed uniformly.

[Prescription Example 2] Lotion (1) Ethanol 15.0 (mass%)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 78.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Extract 1 5.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Prescription Example 3] Cream (1) Squalane 10.0 (mass%)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20 mass% aqueous solution) 15.0
(10) Purified water 36.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Extract 2 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. (11) is added after completion | finish of emulsification, cooling is started, (12) is added at 40 degreeC, and it mixes uniformly.

[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (mass%)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1% by weight aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (derived from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by mass aqueous solution) 2.0
(16) Extract 1 5.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C., add (16) and mix evenly.

[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (mass%)
(2) Purified water 78.7
(3) Sodium hydroxide (10% by mass aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Extract 2 10.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Formulation Example 6] Cleansing Fee (1) Squalane 77.0 (mass%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 3.0
(4) Extract 1 5.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Formulation Example 7] Face-wash foam (1) Stearic acid 16.0 (mass%)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 31.5
(8) Extract 2 6.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the water phase components (5) to (7) are heated and dissolved at 80 ° C., and the oil phase components are uniformly mixed and stirred. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Prescription Example 8] Make-up base cream (1) Squalane 10.2 (mass%)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 65.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Extract 2 5.0
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and uniformly dispersed with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (mass%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 53.6
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Extract 2 5.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Prescription Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Extract 2 5.0
(11) Purified water 43.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C, and gradually add to (4) heated to 50 ° C with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. with stirring, and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

[Prescription Example 11] Pack (1) Purified water 58.9 (mass%)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Extract 1 5.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

[Prescription Example 12] Bath agent (1) Fragrance 0.3 (mass%)
(2) Extract 1 5.0
(3) Sodium bicarbonate 46.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.

[Prescription Example 13] Hair wax (1) Stearic acid 3.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 70.6
(11) Extract 2 5.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 14] Hairtonic (1) Ethanol 46.0 (mass%)
(2) Purified water 48.9
(3) Extract 1 5.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

[Prescription Example 15] Beverage (1) Extract 2 8.0 (mass%)
(2) Erythritol 1.0
(3) Citric acid 0.1
(4) Stevia 0.01
(5) Purified water 90.89
Production method: (1) to (5) are mixed uniformly.

[Prescription Example 16] Tablet (1) Extract 1 0.30 (parts by mass)
(2) Reduced maltose starch syrup 0.53
(3) Corn starch 0.15
(4) Glycerin fatty acid ester 0.02
Production method: (1) to (3) were sieved and mixed, and (4) was further added and mixed. Tableting was performed with a tableting machine to obtain tablets with a total amount of 300 mg.

Claims (3)

A moisturizing agent containing as an active ingredient an extract obtained from the above-ground parts of zazensou. An anti-aging agent comprising as an active ingredient an extract obtained from the ground part of zazensou . Antioxidant containing as an active ingredient an extract obtained from the ground part of zazensou .