JP4097228B2 - New chewing gum - Google Patents

Description

The present invention is a physiologically safe, it relates to a novel chewing gum that shows excellent deodorant effect without sacrificing taste and flavor, the texture.

  Until now, as deodorants derived from natural products, fish and shellfish antioxidants comprising water-soluble or water-insoluble antioxidant components extracted from natural spices such as rosemary or sage (see Patent Document 1), perilla Extraction and decolorization of extract extract or its extract residue with polar solvent and / or non-polar solvent of sage or rosemary, consisting of extract of family plant and solvent extract of extract residue (see Patent Document 2) A deodorant substance obtained by treatment (see Patent Document 3), a Lamiaceae plant or an extract thereof subjected to solvent extraction treatment and alkali treatment, and transferred to a water-insoluble organic solvent layer (Patent Document 4, 5), an oral deodorant (see Patent Document 6), a phenolic compound that combines a deodorizing active ingredient having a chemical scavenging action against a bad breath causing substance and propolis or a solvent extract thereof. Or a deodorant containing a mediator and an enzyme that oxidizes it (see Patent Document 7), a composition for removing bad breath using a sage or rosemary extract and 1-menthol or carvone (see Patent Document 8), Rose Deodorant composition containing at least a natural extract selected from mary, sunflower seed, green coffee bean, tea, grape skin, grape seed, apple extract and an enzyme that oxidizes phenolic compounds Product (see Patent Document 9), gum containing capsules encapsulating laccase (see Patent Document 10), deodorant containing plant-derived deodorant components and basidiomycetes (see Patent Document 11), Lamiaceae plants A deodorant derived from the origin and a substance that inhibits the metabolism of bad breath causing bacteria by Citrus Salamander plants (see Patent Document 12) are known.

However, since these deodorants have a unique taste and aroma, they have the disadvantage of detracting from the original flavor and aroma of foods when formulated in foods. Rosemary extract exhibits a sufficient deodorizing effect. When blended in foods such as chewing gum in an amount that can be done, the bitterness increases due to the effect of carnosol, a deodorant component contained in the chewing gum, the flavor as a chewing gum is impaired, and the spice specific smell remains in the mouth, The aftertaste was bad and the refreshing feeling required for chewing gum was lost.
Moreover, since oil-soluble components such as carnosol contained in this extract have low solubility in saliva in the oral cavity, there is also a drawback that a rough feeling is generated and the texture is lost.

Japanese Patent Laid-Open No. 55-18435 (claims and others) JP-A-57-204278 (Claims and others) Japanese Patent Publication No. 64-1146 (Claims and others) JP 59-6057 (Claims and others) JP 59-6058 (Claims and others) JP 63-264516 A (claims and others) JP 2005-65750 A (Claims and others) Japanese Patent Publication No. 3-69883 (Claims and others) JP-A-10-212221 (Claims and others) JP 2004-321077 A (Claims and others) JP 2004-352621 A (Claims and others) JP 2005-289918 A (Claims and others)

The present invention, flavor inherent chewing gum flavor, without damaging the texture has been made for the purpose of providing a novel chewing gum having excellent deodorizing effect.

Although the present inventors show that the rosemary extract has an excellent deodorizing effect on foods, when it is blended with chewing gum, it has a disadvantage that it exhibits a bitter taste and loses the original flavor and texture of chewing gum. but this is to be attributed to carnosol a deodorant component of the oil-soluble contained therein, therefore, if Re to remove the carnosol that can improve the above disadvantages, deodorization by removal of the carnosol It was found that the decrease in the effect can be supplemented by adding a laccase derived from a specific strain, and the present invention has been made based on this finding.

That is, the present invention provides a laccase derived from basidiomycete belonging to rosemary extracts and (B) Hourokutake genus that does not contain carnosol obtained by removing oil-soluble components from (A) rosemary extract fraction, the mass ratio of 1: 5 In addition, the present invention provides a chewing gum characterized in that a deodorant contained at a ratio of 2: 1 is blended within a range of 0.1 to 2.0% by mass based on the total mass .

で表わされるカルノソール２．５〜３０質量％、通常は５〜１５質量％を含んでいる。 The rosemary extract used as the component (A) of the deodorant in the present invention is an extract extracted from leaves, stems, flowers and other tissues of the perennial plant belonging to the genus Mannerou (Rosmarinus officinalis L.). Solubility formula

It contains 2.5 to 30% by mass, usually 5 to 15% by mass.

As (A) component of this invention, it is necessary to remove this carnosol and to use the rosemary extract which made the content 0 .

  This rosemary extract is made up of rosemary plant tissues such as water, hydrous ethanol, ethanol, acetone, hydrophilic solvents such as acetone, acetic acid, citric acid, acidic solutions, sodium bicarbonate, sodium hydroxide. Extracted with an alkaline aqueous solution or the like. The plant tissue at this time may be raw or dried.

The removal of this carnosol can be carried out by removing the oil-soluble component of the extraction residue by pressure-filtering the suspension of the ethanol extract under reduced pressure or hot water extract, or by adsorbing the oil-soluble component to the adsorption resin. Can be done by removing.

  Next, as the laccase of the component (B), for example, a molecular weight of about 62, which is obtained from a culture solution of a basidiomycete strain (Trametes sp. Hal strain) belonging to the genus Trametes (Trade sp. 000 high heat stability laccase is used. This may be a commercially available powder, for example, “Laccase Daiwa Y120” (product name from Daiwa Kasei Co., Ltd.) or encapsulated in a capsule such as gelatin for stabilization.

The blending ratio of the component (A) and the component (B) in the deodorant used for the chewing gum of the present invention is selected within a range of 1: 5 to 2: 1 as a mass ratio.

The deodorant thus obtained has high safety and excellent deodorizing effect, and when blended in the chewing gum base, does not impair the original flavor, aroma and texture of the gum. It can be suitably used as a deodorant additive. The amount of this time, the range of 0.1 to 2.0 wt% as a extract based on the total weight of the chewing gum is selected.

According to the present invention, there is provided a chewing gum that exhibits an excellent deodorizing effect without detracting from taste, aroma, and texture without harming health.

Next, the best mode for carrying out the present invention will be described with reference to examples, but the present invention is not limited to these.
In addition, the measurement of the deodorizing property in each example was performed by the following method.

(1) Method for measuring component deodorization rate 10 mg of deodorant powder was placed in a 30 ml vial, 5.0 ml of phosphate buffer (pH 7.0) kept at 37 ° C. in advance was added, and the bottle was sealed. Next, 50 μl of a 10 ng / μl dilution of methyl mercaptan (corresponding to 500 ng of methyl mercaptan) was added to the vial and allowed to incubate at 37 ° C. for 10 minutes. Then, 5 ml of headspace gas in the vial was withdrawn with a gas tight syringe, A GC-MS (GC: Agilent 6890A MS: Agilent 5973N) equipped with a nitrogen cold trap device (Gerstel CIS) and a gas analysis HP-PLOTQ column (30 m × 0.32 mm × 20 μm), trap conditions: −120 ° C., 30 Second, splitless, temperature conditions: initial temperature 50 ° C. (hold 1.5 minutes) → 20 ° C. temperature rise / minute → 120 ° C. (hold 5 minutes), flow rate: 1.8 ml / min, the amount of methyl mercaptan was measured .
This methyl mercaptan peak detection is performed by the SIM method in which specific MS fragment ions 47 and 48 derived from the components are selected and detected, and only 1 / 15M phosphate buffer (pH 7.0) is used as a control in a vial. The methyl mercaptan deodorization rate was calculated according to the following formula using the sample charged in
Methyl mercaptan deodorization rate = (%) [(C−S) / C] × 100
C: Mercaptan amount in the control headspace S: Methyl mercaptan amount in the sample headspace

(2) Gum deodorization rate measuring method A mortar is heated with a thermostat so that internal temperature may be 37 degreeC. Separately, 3.5 g of a gum sample was weighed out, added to 10 ml of a phosphate buffer (pH 7.0) previously maintained at 37 ° C., and mixed with the mortar for 10 minutes to prepare a sample. Next, 5 ml of this sample was weighed into a 30 ml vial and tested in the same manner as (1).

Reference example 1
After extracting 1.0 kg of dried rosemary leaves with 9.5 kg of 70% ethanol for 2 hours, the extract is filtered through a filter paper with a water pump (under -650 mmHg pressure). Next, the filtrate was concentrated under reduced pressure at 60 ° C. under a pressure of −650 mmHg, and the concentrate was washed three times with 5 volumes of hot water, and then filtered again through a filter paper under a pressure of −650 mmHg to obtain a residue of oil-soluble components. except for. The filtrate was spray-dried to obtain 67.9 g of rosemary extract having a carnosol content of 0% by mass.
The rosemary extract 10.0 g thus obtained and laccase (manufactured by Daiwa Kasei Co., Ltd., product name “Laccase Daiwa Y120”) 5.0 g are mixed at a mass ratio of 2: 1 of the rosemary extract and laccase. A deodorant was prepared.

Reference example 2
After extracting 1.0 kg of dried rosemary leaves with 7.0 kg of hot water for 2 hours, the extract is filtered through filter paper with a water pump (under -650 mmHg pressure). Subsequently, the filtrate was filtered with a filter paper under a pressure of −650 mmHg, and the concentrated liquid obtained by concentration under reduced pressure at 60 ° C. was spray-dried to obtain 71.0 g of rosemary extract having a carnosol content of 0% by mass.
A deodorant was prepared by mixing laccase with this in the same manner as in Reference Example 1.

Reference example 3
After extracting 1.0 kg of dried rosemary leaves with 8.0 kg of 50% ethanol for 2 hours, the extract is filtered through filter paper with a water pump (under -650 mmHg pressure). Next, the filtrate was passed through a resin (Diaion HP-20), and the concentrated solution obtained by concentrating the passing liquid obtained by adsorbing and removing the bitter substance carnosol at 60 ° C. under a pressure of −650 mmHg was spray-dried to contain carnosol. 100.4 g of a rosemary extract having an amount of 0% by mass was obtained.
A deodorant was prepared by mixing laccase with this in the same manner as in Reference Example 1.

Reference example 4
After extracting 1.0 kg of dried rosemary leaves with 9.5 kg of 70% ethanol for 2 hours, the extract is filtered through a filter paper with a water pump (under -650 mmHg pressure). Next, the filtrate was concentrated under reduced pressure at 60 ° C. under a pressure of −650 mmHg and then dried with hot air at 70 ° C. to obtain 48.9 g of rosemary extract having a carnosol content of 7.5% by mass.

Table 1 shows the carnosol content (% by mass) and the deodorization rate (%) of the rosemary extracts alone of Reference Examples 1 to 4 prepared as described above.
Table 2 shows the deodorization rate (%) of the mixture of rosemary extract and laccase.

  Using the method usually used for the production of chewing gum, the chewing gum having the formulation shown in Table 3 was produced, the deodorization rate was measured, and the results are shown in Table 7.

In the same manner as in Example 1 , chewing gums having the formulations shown in Table 4 were produced. Table 7 shows the deodorization rate.

In the same manner as in Example 1 , chewing gums having the formulations shown in Table 5 were produced. Table 7 shows the deodorization rate.

Comparative Example 1
In the same manner as in Example 1 , chewing gums having the formulations shown in Table 6 were produced. Table 7 shows the deodorization rate.

Examples 1, 2, 3 and comparative examples by 10 panelists (girls: 18, 22, 30, 38, 45, boys; 20, 25, 35, 42, 55) The chewing gum produced in 1 was subjected to a sensory test for taste, aroma, and texture. As a result, ◯ was evaluated as good by 5 or more people, ◯ when 1 or more and 4 or less were good, and × when there was no good at all. The results are shown in Table 8.

The chewing gum of the present invention is useful as a chewing gum for deodorization .

Claims (2)

(A) Rosemary extract containing no carnosol obtained by removing oil-soluble components from rosemary extract, and (B) basidiomycete-derived laccase belonging to the genus Horotake mushroom in a mass ratio of 1: 5 to 2: 1 A chewing gum characterized by containing the deodorant contained in the range of 0.1 to 2.0 mass% based on the total mass . 2. The chewing gum according to claim 1, wherein the component (B) is a laccase obtained from a culture solution of a basidiomycete Hal strain (Trametes sp. Hal strain) belonging to the genus Horotake.