JP2009136240A - Chewing gum composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a chewing gum composition which is excellent in an effect of suppressing foul breath, exhibits high effect of suppressing foul breath even after long preservation in a high temperature condition, and excellent in appearance stability. <P>SOLUTION: This chewing gum composition includes (A) rosemary or its extract, (B) polyphenol oxidase, and (C) a plant of Pyrolaceae Chimaphila umbellata or its extract. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a chewing gum composition that is excellent in halitosis suppression effect, retains a high halitosis suppression effect even when stored for a long time under high temperature conditions, prevents discoloration, and has good appearance stability.

  The bad breath is caused by methyl mercaptan, hydrogen sulfide, or the like generated by decomposition of oral dirt (food residue, exfoliated mucous membrane, etc.) by the bacteria that cause bad breath. For prevention of bad breath, (1) capture and deodorization of methyl mercaptan, (2) removal of bad breath causing bacteria, (3) inhibition of enzymes that cause bad smell of bad breath causing bacteria (bacterial metabolism inhibition), (4) There are methods such as removal of dirt by promoting saliva secretion, and many effective deodorants have been reported for (1), which is particularly important. In particular, there are many knowledges about deodorant compositions derived from plants, such as polyphenols derived from immature fruits of rose family (see Patent Document 1), Lamiaceae plant solvent extracts (see Patent Document 2), camellia family extracts (Patent Document 3). For example).

  Furthermore, as a technique related to (1), as a deodorant having strong deodorizing power and considering safety and the environment, OH-OH, A deodorant (see Patent Document 4) that exhibits a high deodorizing effect when used in combination with an oxidoreductase such as a diphenol donor, and a Lamiaceae plant extract is higher than methyl mercaptan. It has been proposed that it exhibits a deodorizing effect and is effective as an oral deodorant (see Patent Document 5). Further, it has been proposed that a phenolic compound such as catechol has a deodorizing effect, and that the deodorizing effect is improved by using polyphenol oxidase in combination with this compound (see Patent Document 6).

However, it is known that polyphenol substances are likely to cause discoloration, and catechol oxidase, which is one of polyphenol oxidases, governs the browning phenomenon of the cut surface of fruits (see Patent Document 7). Furthermore, there is a problem that the oxidoreductase tends to be inactivated, and a technique for stably blending the gum into the gum is proposed by encapsulating and encapsulating laccase to prevent inactivation in the gum base over time ( However, the stabilization effect by this encapsulation is not sufficient.
By combining the extract of Lamiaceae plants and salamander seeds in the oral composition, the quick-acting and sustainability of the bad breath suppression effect is improved, and when tricoryenol and / or laccase is used in combination It has also been proposed that a high halitosis suppression effect is exhibited (see Patent Document 9), but the effect still has room for improvement. Moreover, although the formulation which combined the polyphenol substance and polyphenol oxidase is marketed, the thing of satisfactory usability cannot be confirmed.

  In this way, in the preparation having halitosis suppression effect, the halitosis suppression effect and its sustainability and stability are still not sufficient, exhibit higher halitosis suppression effect, and stable effect even after long-term storage It is desirable to develop a technology that can obtain a desired formulation.

JP-A-7-285876 Japanese Patent Publication No. 1-1145 Japanese Examined Patent Publication No. 4-41076 Japanese Patent Publication No. 7-53174 JP-A-57-204278 JP-A-9-38183 JP 2001-161357 A JP 2004-321077 A JP 2005-289918 A

  The present invention has been made in view of the above circumstances, and an object thereof is to provide a chewing gum composition that is excellent in bad breath suppression effect, exhibits high bad breath suppression effect even after long-term storage under high temperature conditions, and has good appearance stability. And

  As a result of intensive studies to achieve the above-mentioned object, the present inventor has achieved a high suppression of bad breath by using rosemary or an extract thereof, polyphenol oxidase, and a plant of the genus Euphorbiaceae or an extract thereof. It was found that a chewing gum composition having an effect and exhibiting a high halitosis suppression effect even when stored under high temperature conditions and having good appearance stability was obtained, and the present invention was made.

That is, it is well known that plants such as Labiatae or their solvent extracts and phenolic compounds have a deodorizing effect. Furthermore, it is known that when an oxidoreductase is used in combination, the deodorizing effect is improved, but the deodorizing effect is not satisfactory. In addition, it is difficult to allow oxidoreductase and its substrate phenolic compound to coexist in a stable state, the enzyme is deactivated during long-term storage, the deodorization rate is lowered, and the preparation is discolored by polyphenol. There was a problem that was recognized. In particular, it was difficult to stably mix these components in the gum base. In addition, there is a problem that the enzyme is likely to be inactivated during the preparation process of the preparation or during storage such as the distribution process.
On the other hand, in the present invention, the chewing gum composition in which rosemary or an extract thereof, polyphenol oxidase, and a plant of the genus Euphorbia sp. Or an extract thereof is synergistically improved in suppressing bad breath, and high bad breath suppression The effect is exhibited, and the enzyme is hardly inactivated even when stored for a long time under high temperature conditions, the deodorizing effect is maintained, discoloration of the preparation can be prevented, and the appearance stability is excellent.

  Accordingly, the present invention provides a chewing gum composition comprising (A) rosemary or an extract thereof, (B) polyphenol oxidase, and (C) a plant belonging to the genus Umegasoma or an extract thereof. Offer things.

  The chewing gum composition of the present invention is excellent in halitosis suppression effect, has good appearance stability even when stored under high temperature conditions, and maintains a high halitosis suppression effect, for prevention or suppression of halitosis Useful as a formulation.

  Hereinafter, the present invention will be described in more detail. The chewing gum composition of the present invention is characterized by containing (A) rosemary or an extract thereof, (B) polyphenol oxidase, and (C) a plant of the genus Euphorbia genus or an extract thereof.

The rosemary which is the raw material plant of the component (A) rosemary or its extract is represented by the Japanese name “Mannenrou” and the scientific name “Rosmarims of ficinalis”, and conventionally known ones can be used.
Specifically, the rosemary can be obtained by pulverizing and powdering rosemary leaves and stems as raw materials. In addition, as the extract, the above-mentioned rosemary in the form of powder is polar solvent and / or nonpolar solvent (for example, water, ethyl ether, ethylene chloride, dioxane, acetone, ethanol, methanol, ethyl acetate, propylene glycol, etc. Extract obtained by extraction with a polar solvent or a non-polar solvent such as n-hexane, petroleum ether, ligroin, cyclohexane, carbon tetrachloride, chloroform, dichloroethane, toluene, benzene, or a mixed solvent thereof) and its extraction Extract obtained by subjecting a raw material selected from the residue to solvent extraction, as well as carnosol, carnodic acid, 7,11,12-trihydrokioshi-6,10- (epoxymethano) abieta isolated from the above plant -8,11,13-triene-20-one (Ros Nord), and deodorizing active ingredients (JP 59-203445 discloses such a salt thereof, JP-57-204278, JP-see JP-A-59-103665) can be used. The solvent used in the solvent extraction treatment may be an organic solvent or an inorganic solvent as described in the above publication, or may be a mixed solvent of an organic solvent and an inorganic solvent, and is extracted by a normal extraction method. Can do.

  Specific examples of the organic solvent include ethyl ether, ethylene chloride, dioxane, acetone, ethanol, methanol, ethyl acetate, propylene glycol, butylene glycol and other polar solvents, n-hexane, petroleum ether, ligroin, cyclohexane, carbon tetrachloride, Nonpolar solvents such as chloroform, dichloroethane, toluene, benzene and the like can be mentioned. Examples of the inorganic solvent include aqueous solutions of water, acid, alkali, or salt. Specifically, hydrochloric acid, sulfuric acid, phosphoric acid, nitric acid, carbonic acid, sodium hydroxide, potassium hydroxide, ammonium hydroxide, calcium hydroxide Sodium chloride, potassium chloride, ammonium chloride, sodium bicarbonate, sodium carbonate, sodium sulfate and the like. These acids, alkalis and salts are preferably used at a concentration of 2 mol or less. Preferred solvents are water, lower alcohols having 1 to 3 carbon atoms, and polyhydric alcohols. Moreover, it is preferable that the usage-amount of the said solvent shall be 1 time or more by mass ratio with respect to a rosemary raw material.

As the extract of rosemary as component (A), the extract of rosemary as described above may be blended as it is, but if necessary, after removing solids by filtration or centrifugation, Alternatively, the solvent may be distilled off for concentration or drying. Furthermore, you may mix | blend what adjusted the rosemary extract obtained as needed to the appropriate density | concentration by diluting with the same kind or different solvent similar to the above-mentioned solvent. In addition, when diluting with a solvent, the concentration range of the rosemary extract is preferably 0.1 to 80% (mass%, the same applies hereinafter), particularly 1 to 60%.
Furthermore, a rosemary extract or a diluted product thereof can be formulated by adding sugar alcohols such as dextrin, erythritol, mannitol, fats and oils, and the like. ~ 90% is desirable.

  As the rosemary extract of component (A), a commercially available product can be used, and it is not particularly limited. However, Reomir RMW (10% rosemary water-soluble extraction) sold by Toyoda Fragrance Co., Ltd. Products), dextrin-shaped preparations such as Reomile WL (30% rosemary ethanol extract), and oily preparations such as Reomile EK5-N (Rosemary 20% hexane extract) sold by Nippon Stange Co., Ltd. are preferably used. it can.

  (A) The amount of rosemary or an extract thereof is preferably 0.0001 to 20%, particularly 0.001 to 1.0%, particularly 0.01 to 0% of the whole composition in terms of pure content. .5% is preferred. If it is less than 0.0001%, a satisfactory effect may not be exhibited. If it exceeds 20%, the stability or flavor of the composition may be impaired.

Examples of (B) polyphenol oxidase include catechol oxidase (EC 1.10.3.1), laccase (EC 1.10.3.2), monophenol oxidase (EC 1.14.18.1), and the like. Laccase is particularly preferred. The polyphenol oxidase may be of microbial origin or plant origin.
The polyphenol oxidase can be used as it is, or an appropriate solvent such as water, a buffer having a buffering action at pH 4 to 9 (acetic acid, citric acid, phosphoric acid, boric acid, glycine, veronal buffer, etc.), alcohol, etc. It may be used after being dissolved in the above-mentioned solvent containing. When polyphenol oxidase is dissolved in a solvent and used, the concentration of polyphenol oxidase is preferably 0.1 to 80%, particularly 1 to 60%.

  As the polyphenol oxidase, a commercially available product can be used. For example, laccase is not particularly limited, but “Laccase Daiwa (dextrin supplementation) derived from white rot fungus (Trametes sp.) Commercially available from Amano Enzyme Inc. Form preparation) ".

  The compounding amount of polyphenol oxidase is preferably 0.00001 to 10%, particularly 0.0001 to 1.0%, particularly 0.0005 to 0.5% of the whole composition in terms of pure content. If it is less than 0.00001%, a satisfactory effect may not be exhibited, and if it exceeds 10%, the stability or flavor of the composition may be impaired.

  Further, the blending ratio of the component (A) and the component (B) is preferably a range of 1 to 20, particularly 1 to 8, especially 1 to 2 in terms of a mass ratio when converted into a pure component. . If (A) / (B) is less than 1, a satisfactory bad breath suppressing effect cannot be exhibited, and even if (A) / (B) exceeds 20, a satisfactory bad breath suppressing effect may not be exhibited.

  The component (C) is a plant belonging to the genus Umegasausaceae or an extract thereof. As the plant belonging to the genus Umegasausae, Euphorbia is preferred. Euphorbia is a scientific name (PIPSSISSEWA) Chikumaphila umbelate W. It is a Barton plant and a perennial herb that grows naturally in a dry forest near the seashore. It originates in temperate zones such as North America, Europe, and Siberia, and is distributed north of Ibaraki Prefecture in Japan.

  In the present invention, the whole plant, leaves, stems, roots, seeds and the like of plants belonging to the genus Euphorbia can be pulverized into powders or extracts thereof. In addition, the powder of the plant of the genus Euphorbia or its extract has been used as a fragrance for herbal tea or candy that has been ingested by humans for a long time in the United States, etc., and there is no problem with regard to safety to the human body. It is a material.

  As an extract of the plant of the genus Euphorbia, it may be extracted by a known extraction method using a raw or dried plant as it is or using a pulverized powder as a raw material. In this case, as a raw material for solvent extraction, in addition to the above raw materials, an extract or an extraction residue obtained by solvent extraction can also be used.

  Examples of the extraction solvent include water, alcohols (for example, lower monohydric alcohols such as methanol and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, and ethyl acetate. Examples include esters, diethyl ether, dioxane, acetonitrile, xylene, benzene, chloroform and the like. These solvents can be used alone or in a mixture of two or more. Moreover, when extracting from an extraction residue again, not only the same solvent but a different solvent may be used.

  When obtaining a plant extract belonging to the genus Euphorbia, the ratio of the raw material plant to the solvent is not particularly limited, but the solvent is preferably used in a mass ratio of 5 to 99 times that of the plant raw material. The extraction temperature is preferably in the range of room temperature to the boiling point of the solvent under normal pressure. As the extraction operation method, a general method can be employed. For example, a dipping method, a countercurrent extraction method, or the like can be employed, and stirring may be used in combination as necessary. A solid can be separated from the extraction mixture to obtain an extract, and this extract and its concentrate can be used.

  Further, the obtained extract may be subjected to treatment such as partitioning or purification by various chromatographies or decolorization with activated carbon or the like, if necessary. As a deodorizing operation, an adsorption method using activated carbon, a steam distillation method, a deodorizing method by concentrating an aqueous solution, a supercritical fluid extraction method using carbon dioxide, a distribution method with an organic solvent, or the like can be employed.

The plant of the genus Euphorbia or its extract may be subjected to a drying step such as spray drying or freeze drying, or a concentration step such as normal pressure, reduced pressure, or a membrane, depending on the application. Moreover, you may dilute and use an extract.
In this case, the diluting solvent is not limited, and the same solvents as those used for diluting the rosemary or the extract thereof can be used. Preferred solvents are water, lower alcohols having 1 to 3 carbon atoms. Or a polyhydric alcohol such as propylene glycol or 1,3-butylene glycol, and when diluted, the concentration range of the plant extract of the genus Euphorbia is preferably 0.01 to 80%, particularly preferably 0.1 to 60%. .

  Furthermore, if necessary, a dextrin powder, sugar alcohols such as erythritol, mannitol, and fats and oils may be added, mixed and shaped, and the amount of excipient added is 10 to 90%. preferable.

  As the plant extract belonging to the genus Euphorbia, a commercially available product can be used, and is not particularly limited, but for example, Euphorbia leaf extract manufactured by Ikeda Sakuka Kogyo Co., Ltd. can be more preferably used.

  The compounding amount of the extract of the genus Euphorbia is preferably 0.001 to 20%, particularly 0.001 to 10%, particularly 0.01 to 1% of the whole composition in terms of pure content. If it is less than 0.001%, a satisfactory bad breath suppression effect may not be exhibited. If it exceeds 20%, the stability or flavor of the composition may be impaired.

The chewing gum composition of the present invention may be any of chewing gum compositions such as taste gum, bubble gum, and sugar-coated gum. The above ingredients are blended, and a gum base, a coloring agent, a sweetener, a fragrance, and the like are blended. It can be manufactured by the method.
As the gum base, commonly used polyvinyl acetate resins having a degree of polymerization of 100 to 1000, natural resins (such as chicle, gelton, and solver), polyisobutylene, polybutene, ester gum, and other basic agents, calcium carbonate, calcium phosphate, and talc In addition, fillers such as lanolin, stearic acid, sodium stearate, potassium stearate, glyceryl triacetate, glycerin and other plasticizers or softeners, as well as natural wax, petroleum wax, paraffin wax and the like can be incorporated into the gum base. The blending amount of the gum base is preferably 10 to 50%, particularly 15 to 35% of the entire composition. As the colorant, natural pigments such as gardenia, safflower, sanitary beef, titanium dioxide and the like are used.

  Examples of the sweetener include commonly used sweeteners such as sucrose, glucose, dextrose, invert sugar, fructose and other sugars, xylitol, erythritol, sorbitol, mannitol, maltitol, lactitol, palatinose, palatinit, trehalose, oligosaccharide, reduced starch syrup. , Stevia, sucralose, saccharin, and aspartame are preferable, and xylitol, maltitol, sucralose, and paratinite are preferably included from the viewpoint of flavor. The blending amount of the sweetener is desirably 10 to 75%, particularly 15 to 70% of the whole composition.

  As the fragrance, it is preferable to use one or more kinds of oil and fat fragrances and powder fragrances such as natural fragrances and synthetic fragrances, but there is no particular limitation. For example, natural flavors such as mastic oil, parsley oil, anise oil, eucalyptus oil, wintergreen oil, cassia oil, menthol oil, spearmint oil, peppermint oil, lemon oil, coriander oil, orange oil, mandarin oil, lime oil, lavender Oil, laurel oil, chamomile oil, cardamom oil, caraway oil, bay oil, lemongrass oil, pine needle oil, neroli oil, rose oil, jasmine oil, Iris concrete, absolute peppermint, absolute rose, orange flower, citrus oil, Examples include mixed fruit oil, strawberry oil, cinnamon oil, clove oil, and grape oil.

  Single flavors include carvone, anethole, methyl salicylate, cinnamic aldehyde, linalool, linalyl acetate, limonene, menthone, menthyl acetate, pinene, octyl aldehyde, citral, pregon, carbyl acetate, anisaldehyde, ethyl acetate, butyl butyrate, Allylcyclohexanepropionate, methylanthranilate, ethylmethylanthranilate, vanillin, undecalactone, hexanal, ethinone alcohol, propyl alcohol, butanol, isoamyl alcohol, hexenol, dimethylsulfide, cycloten, furfural trimethylpyrazine, ethyl Examples include lactero and ethyl lioacetate. Single flavor and / or natural flavors including strawberry flavor, apple flavor, banana flavor, pineapple flavor, grape flavor, mango flavor, tropical fruit flavor, butter flavor, milk flavor, yogurt flavor, fruit mix flavor, herbal mint Flavors and the like. Moreover, the form of a fragrance | flavor may be an essential oil, an extract, a solid substance, or the powder which spray-dried these. The blending amount of the fragrance is preferably 0.001 to 15% of the whole composition, particularly preferably 0.001 to 10%. If it is less than 0.001%, a satisfactory effect is not exhibited. The flavor and texture may be impaired.

  Furthermore, the chewing gum composition of the present invention may contain other additives such as acidulants, brighteners, thickeners, emulsifiers, pH adjusters, preservatives, etc., as necessary, without impairing the effects of the present invention. You may add suitably in the range.

  The chewing gum composition can be produced, for example, by first adding a predetermined amount of the above-mentioned components (A) to (C), sweeteners, fragrances, and coloring agents to a gum base mainly composed of a resin for gum base and comprising a wax, an emulsifier and a filler Then, additives such as a softener and a taste-masking substance are added, and the mixture is uniformly kneaded using a kneader at around 50 ° C. Next, processing such as plate shape or block shape is performed to form gum, bubble gum or the like. Thereafter, a sugar-coated gum may be formed by spraying and coating sugar alcohol syrup.

  The chewing gum composition of the present invention can remove bad breath, and examples of bad breath substances include sulfur-containing compounds such as mercaptans or indole, skatole, ammonia, urea, amines, other nitrogen-containing compounds, etc. There is.

  The chewing gum composition of the present invention can exert a deodorizing effect by chewing the chewing gum composition in the oral cavity and causing the malodorous substances to react with the components (A), (B) and (C). Since the reaction of the components (A), (B) and (C) proceeds smoothly when water is present together, a chewing gum base material capable of secreting a large amount of saliva is suitable.

  In this case, any temperature may be used as long as the enzyme reaction proceeds, and depending on the type of enzyme, it may be within the normal oral temperature range, and when mixed at 37 ° C., the reaction proceeds rapidly, which is preferable. . The required time varies depending on the kind and amount of enzyme used, but it is usually sufficient to chew in the mouth for several minutes. Other conditions are not particularly limited as long as they are set in an environment in which the enzyme reaction proceeds.

  EXAMPLES Hereinafter, although an Example and a comparative example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to the following Example. In addition, unless otherwise indicated,% shown below means the mass%.

Examples and comparative examples
Chewing gum compositions having the compositions shown in Tables 1 to 4 were prepared and evaluated by the following methods.
<Preparation of chewing gum composition>
According to the composition shown in Tables 1-4, gum base (manufactured by Natural Veil Co., Ltd.), maltitol (manufactured by Towa Kasei Co., Ltd.), xylitol (manufactured by Rocket Fleure Co., Ltd.), and then 10% rosemary water extract (* 1) Add Persianum extract (* 2), powder flavor, oil flavor, laccase (* 3), and knead uniformly at around 50 ° C using a 1 L capacity kneader (TKI-1 manufactured by Toshin Co., Ltd.) 800 g were produced. Then, it was set as the gum which gave the plate-shaped process of thickness 1cm. In addition, the rosemary extract, laccase, persilla extract, catechin powder (* 4), and salamander extract (* 5) used in each example are as follows.

(* 1) Toyome Fragrance Co., Ltd. Reomir RMW (10% rosemary water-soluble extract shaped with dextrin powder)
(* 2) Ikeda Sakka Kogyo Co., Ltd. Omega Megasou extract (water extract of Omega Megaspodium dissolved in 50% aqueous solution of butylene glycol to obtain a concentration of 1% Omega Sasso)
(* 3) Laccase Daiwa Y120 manufactured by Amano Enzyme Co., Ltd. (30% laccase shaped with dextrin powder)
(* 4) Polyphenon KN (100% catechin water extract dry powder) manufactured by Mitsui Norin Co., Ltd.
(* 5) Nippon Tablet Co., Ltd. Sansho Extract F-1 (40% sunshade shaped with erythritol)

<Experiment 1> Evaluation of bad breath suppression effect For the evaluation of bad breath, the UBC (British Columbia University) sensory evaluation method by one expert of odor discrimination judgment (odor judgment person) was adopted. The breath collection method is as follows. First, mouth breathing is performed three times, then the mouth is closed for 1 minute, and only the breath accumulated in the mouth is blown to the odor judge through a predetermined plastic pipe. Evaluation was made on a 6-point scale.

Score (evaluation)
5 (××) Intense odor 4 (×) Strong odor 3 (△ ～ ×) Easily detectable odor 2 (△) Detectable but weak odor 1 (○) Finally sensible odor 0 (◎) No odor

  Exhalation after 30 minutes without brushing teeth was determined to have a bad breath of 4 or more according to the above evaluation method, and 20 non-smokers 20 to 45 years old were subjects. Ten subjects were stratified into two groups, group A and group B, so that the average values were equal. Ten people in Group A chewed 2 g of the chewing gum compositions of Examples shown in Tables 1 and 2, and 10 people in Group B chewed 2 g of the chewing gum compositions of Comparative Examples shown in Tables 3 and 4, respectively. The bad breath of each test subject afterward was determined by the same evaluation method as before the start of the test. Two points or less were judged to have a high bad breath suppression effect.

<Experiment 2> Evaluation of appearance stability (discoloration) during high-temperature storage 40 pieces of chewing gum cut into 2 g plates were placed in a 200 mL container manufactured by Toppan Printing Co., Ltd., covered, and a 40 ° C. constant temperature bath (Yamato Scientific Co., Ltd.) Company incubator IG420) as a control, it was left in a 5 ° C. constant temperature bath (Yamato Scientific Co., Ltd. incubator IG400), and the degree of appearance stability (discoloration) after 2 months was evaluated in the following 5 levels.

Score (evaluation)
◎ No discoloration compared to the control ○ A slight discoloration compared to the control, but a level that is difficult to identify by itself and no problem △ Discoloration that can be easily identified compared to the control × Compared to the control Significant discoloration is observed, giving a sense of unsanitation

<Experiment 3> Removal effect of methyl mercaptan under severe conditions 50 g of chewing gum was put into a 200 mL container of Toppan Printing Co., Ltd., covered, and left in a constant temperature bath (Yamato Scientific Co., Ltd. Incubator IG420) at 2 months. Methyl mercaptan deodorizing effect was measured by the following method.
A sample solution was prepared by adding 2 g of gum to 10 mL of 0.1 M phosphate buffer at pH 6.5 and mixing well in a mortar for 10 minutes. Add 0.2 mL of the sample solution to a 30 mL test tube, heat to 37 ° C., and then add 0.5 mL of ice-cooled 0.0001% methyl mercaptan solution and 0.1 M phosphate buffer solution at pH 7.5. 5 mL was added, stirred for 5 seconds, sealed, and reacted in a 37 ° C. water bath for 6 minutes. 5 mL of air in the head space of the test tube was collected and measured by gas chromatography (specimen solution measurement value A).
As a control sample, 0.2 mL of only 0.1 M phosphate buffer was prepared, and gas chromatography measurement was performed in the same manner as described above (measurement value of control sample: C). The setting conditions for gas chromatography are as follows.

Measuring instrument: Gas chromatography GC14A
Recorder: C-R4A CHROMATORAC (manufactured by Shimadzu Corporation)
Detector: FPD (Frame Photometric detector;
(Shimadzu)
Hydrogen gas generator: HYDROGEN GENERATOR HG260
(GL Sciences)
Column: DNP 20% Chromosorb WAW,
DMCS 60/80 Mesh, φ3.2mm × 1mm
Column temperature: 40 ° C. (4 min) to (30 ° C./min) to 130 ° C. (16 min)
Inlet temperature: 130 ° C
Carrier gas: nitrogen (pressure control 1.75 kg / cm ² ),
(Mass flow control 0.5kg / cm ² )

From the measured values, the methyl mercaptan deodorization rate was determined by the following formula.
Methyl mercaptan deodorization rate (%) = ((C−A) / C) × 100
C: Methyl mercaptan peak area of blank solution
A: Methyl mercaptan peak area of sample liquid In addition, it was judged that the deodorizing effect was high about the composition which has a deodorizing rate of 80% or more.

  The above experimental results are shown in Tables 1-4.

  From the results of Tables 1 to 4, when any of the components (A) to (C) is lacking, the bad breath suppression effect is low, and the appearance stability after high temperature storage and the deodorizing effect are inferior. The chewing gum composition used in combination with the components (A) to (C) of the invention exhibits a remarkably high bad breath suppression effect, has almost no discoloration after high-temperature storage, has excellent appearance stability, and has a high deodorizing effect. confirmed.

Claims (1)

  A chewing gum composition comprising (A) rosemary or an extract thereof, (B) polyphenol oxidase, and (C) a plant belonging to the genus Umegasauria or an extract thereof.