JP4026002B2 - Analysis apparatus and analysis method - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an analyzer provided with a plurality of flow paths each having a reaction chamber that houses solid particles, and an analysis method using the analyzer.
[0002]
[Prior art]
In recent years, in the field of analytical chemistry, research on micro technology (Micro Electro-Mechanical System, hereinafter referred to as MEMS) has progressed rapidly, and micro-measurement of an analyzer (μ-TAS; The movement of Micro Total Analytical System or Lab ona chip) has spread to analysis fields such as proteins, genes, biochemistry, etc., and research is being carried out.
[0003]
As a bio-MEMS analyzer that performs an immune reaction, an enzyme reaction, and the like, for example, an attempt to analyze using a microchip with solid fine particles as a reaction solid phase has been reported (for example, see Non-Patent Document 1). According to this method, by introducing solid particles such as beads as the reaction solid phase, the surface area on which the antibody is immobilized can be increased, and analysis with higher sensitivity is possible.
[0004]
[Non-Patent Document 1]
“Development of a dioxin measurement system in flue using BioMEMS, report on the results (first year)”, New Energy and Industrial Technology Development Organization, March 2002 [0005]
[Problems to be solved by the invention]
However, the analyzer described in Non-Patent Document 1 describes the measurement of each sample, and no special device for measuring a plurality of samples at the same time is made. Therefore, in the bioanalysis that requires a measurement time of 10 minutes to 1 hour, there is a problem that it takes a processing time to analyze a plurality of samples. Further, in the comparison between the standard substance and the sample to be measured, the measurement time of the sample solution of each concentration is different, so that the reliability may be lowered. In bioanalysis, in order to improve measurement reliability, multiple sample solutions with different dilution rates are prepared based on the sample to be measured, and the analysis results of the measurement sample are calculated from the analysis values for those dilution rates. The method is often used, but in this case as well, analysis with a conventional analyzer takes time, and the measurement time of the sample solution of each concentration is different, so it is difficult to make the measurement conditions uniform and reliable. Could be lower.
[0006]
The present invention has been made in view of the problems of the prior art, and provides an analyzer capable of easily and efficiently measuring a plurality of samples and an analysis method using the analyzer. Is an issue.
[0007]
[Means for Solving the Problems]
As a result of intensive studies to solve the above-mentioned problems, the inventors of the present invention have a plurality of flow paths provided with a reaction chamber for storing solid particles and a detection unit for photoanalyzing a solution flowing out of the reaction chamber. It has been found that the above-mentioned problems can be solved by providing detection means for photoanalyzing each detection unit provided in parallel, and the present invention has been completed.
[0008]
That is, the first invention of the present invention includes a reaction chamber for storing solid particles, a sample liquid introduction section upstream of the reaction chamber, a detection section downstream of the reaction chamber, the reaction chamber, and the sample. An analyzer comprising a plurality of flow paths each having an upstream dam portion formed between a liquid introduction portion and a downstream dam portion formed between the reaction chamber and the detection portion, The upstream weir portion and the downstream weir portion of each of the flow paths are weir portions for blocking the solid particles and allowing the liquid to pass therethrough , and the detection sections of the plurality of flow paths are each provided with an optical fiber. A first detection unit connected to the second detection unit, and a second detection unit photographed by a CCD camera; The second detection unit is formed within a distance enabling photographing with a CCD camera. It is an analytical device according to claim which are formed in parallel in proximity to have.
[0009]
In the first invention, the upstream weir portion and the downstream weir portion of each of the plurality of flow paths are each narrower than the particle diameter of the solid particles and substantially the same depth as the reaction chamber in the flow direction. The formed slit-like weir portion is preferable.
[0010]
The analyzer of the first invention may include a laser light source and a detector, and an optical fiber that communicates the laser light source and the detector with each of the detection units of the plurality of flow paths. Further, a laser light source, a polygon mirror for scanning the laser light from the laser light source and dispersing the laser light in the detection units of the plurality of flow paths, and a CCD camera for photographing the detection unit may be provided. Further, a laser light source, a cylindrical lens for dispersing the laser light from the laser light source to each detection unit of the plurality of flow paths, and a CCD camera for photographing the detection unit may be provided.
[0012]
The analyzer according to the first aspect of the present invention is provided with a reaction chamber for storing solid particles, and an upstream weir portion and a downstream weir portion for blocking the solid particles and allowing liquid to pass therethrough . Thereby, it is possible to prevent the solid particles from flowing out of the reaction chamber and to efficiently perform the reaction using the solid particles as the reaction solid phase. Further, the slits formed in the flow direction at the upstream weir portions and the downstream weir portions of the plurality of flow paths, each having a width narrower than the particle diameter of the solid particles and substantially the same depth as the reaction chamber. By using the dam-like weir portion, it is possible to prevent the sample liquid or the like from staying on the bottom surface of the reaction chamber. Further, such a weir portion is preferable because it can be easily manufactured by processing from the surface.
[0013]
A second invention of the present invention is an analysis method using the analyzer, wherein a measurement target component in a sample solution or a complex obtained by complexing the solid particle is immobilized on the solid particle, and the measurement target is immobilized. An analysis method characterized by reacting a component or complex with a coloring reagent solution, and optically analyzing the coloring reagent solution colored by this in a plurality of detection units arranged in parallel.
According to the analysis method of the second invention, analysis of a plurality of samples and the like can be performed easily and efficiently in a short time.
[0014]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, embodiments of the present invention will be described with reference to the drawings. In addition, this embodiment is for demonstrating the summary of this invention, and does not limit this invention unless there is particular limitation.
[0015]
1 and 2 show an embodiment of a microchip included in an analyzer according to the present invention. FIG. 1 is a schematic plan view of a substrate portion, and FIG. 2 is a schematic plan view of a lid portion. The microchip 1 of the present embodiment is generally configured by laminating a lid 3 on a substantially flat plate-like substrate 2 and integrating them by anodic bonding, and the overall shape is substantially plate-like.
[0016]
As the substrate 2, a flat plate made of silicon or quartz glass is used, and a silicon substrate is preferably used.
As the material of the lid 3, Pyrex (registered trademark) glass or quartz glass is used, and preferably, Pyrex glass is used.
Here, a combination using a silicon base material as the substrate 2 and Pyrex glass as the lid is preferable because it can be easily integrated by anodic bonding.
[0017]
The substrate 2 is etched to form a plurality of linear grooves. That is, as shown in FIG. 1, four thin line-shaped grooves having a rectangular cross section are formed in parallel to form flow paths 10, 20, 30, and 40.
The flow path 10 includes, in order from the upstream side, a sample solution introduction unit 11, an upstream dam unit 12, a reaction chamber 13, a downstream dam unit 14, a first detection unit 15, a second detection unit 16, and a sample solution outflow unit 17. Have.
In the reaction chamber 13, a groove having a rectangular cross section is formed in a straight line so that a large number of solid particles P are accommodated. An upstream dam portion 12 and a downstream dam portion 14 are provided on the upstream side and the downstream side of the reaction chamber 13. The upstream weir portion 12 and the downstream weir portion 14 are slits having a rectangular cross section whose depth is substantially the same as the depth of the reaction chamber 13 along the flow direction and each width is narrower than the diameter of the solid particles P. However, a large number of the reaction chambers 13 are formed substantially uniformly over the entire width side of the reaction chamber 13. On the downstream side of the downstream weir unit 14, a first detection unit 15 serving as a site for analysis using an optical fiber and a second detection unit 16 serving as a site for analysis using a CCD camera are provided.
Similarly to the flow channel 10, the flow channels 20, 30, and 40 are sample solution introduction parts 21, 31, 41, upstream dam parts 22, 32, 42, reaction chambers 23, 33, 43 in order from the upstream side. , Downstream weir portions 24, 34, 44, first detection portions 25, 35, 43, second detection portions 26, 36, 46, and sample solution outflow portions 27, 37, 47 are provided.
[0018]
In addition, a solid particle flow path 50 that is a thin line-shaped groove having a rectangular cross section is formed on the upstream side of the reaction chambers 13, 23, 33, and 43 of the flow paths 10, 20, 30, and 40. The solid particle flow path 50 is composed of a single flow path 50a on the upstream side, and then the flow paths 50b and 50c branched into two toward the downstream, and the most downstream side of the flow paths 50b and 50c. Each of the flow paths 50d, 50e, 50f, and 50g is branched into two. The ends of the flow paths 50d, 50e, 50f, and 50g are connected to the upstream ends 13a, 23a, 33a, and 43a of the reaction chambers 13, 23, 33, and 43, respectively.
[0019]
Next, as shown in FIG. 2, the lid 3 has holes 11b, 15b, 17b, 21b, 25b, 27b, 31b, 35b, 37b, 41b, 45b, 47b, 51b, and 52b penetrating from the front surface to the back surface. 14 holes are formed. The hole 11b is a connection part 11a at the upstream end of the sample liquid introduction part 11 of the substrate 2, the hole 15b is a connection part 15a at the substantially central part of the first detection part 15 of the flow path 10, and the hole 17b is a sample liquid outflow part 17. Are connected to the connecting portion 17a at the downstream end. Similarly, the holes 21b, 25b, and 27b are the connection portions 21a, 25a, and 27a of the substrate 2, the holes 31b, 35b, and 37b are the connection portions 31a, 35a, and 37a of the substrate 2, and the holes 41b, 45b, and 47b are the substrate. The two connection portions 41a, 45a, 47a and the holes 51b, 52b communicate with the connection portions 51a, 52a of the substrate 2, respectively.
[0020]
A glass fiber tube joint 11 c for pipe connection is bonded on the hole 11 b of the lid 3 to form a liquid introduction path communicating with the upstream end 11 a of the flow path 10. Similarly, glass fiber tube joints 21c, 31c, and 41c are bonded to the holes 21b, 31b, and 41b, respectively, to form a liquid introduction path. Similarly, glass fiber tube joints 17c, 27c, 37c, and 47c are bonded to the holes 17b, 27b, 37b, and 47b, respectively, to form a liquid discharge path. Further, similarly, the glass fiber tube joints 51c and 52c are bonded to the holes 51b and 52b, respectively, to form a solid particle introduction / discharge path.
A plastic holder 15c for connecting an optical fiber is adhered on the hole 15b of the lid 3, so that the optical fiber is connected to the first detection unit 15 of the flow path 10. Similarly, plastic holders 25c, 35c, and 45c are bonded to the holes 25b, 35b, and 45b, respectively, so that the optical fibers are connected and connected.
[0021]
Furthermore, the analyzer of the present embodiment is provided with means for analyzing the first detection unit and the second detection unit of each flow path of the microchip 1 described above.
That is, optical fibers are connected to the first detectors 15, 25, 35, and 45, and each optical fiber is connected to a laser light source and a detector (not shown) via a joint.
Further, as shown in FIG. 3, a polygon mirror 70 is installed above the second detectors 16, 26, 36, 46, and scans light from a laser light source 60 provided separately. ing. In addition, a CCD camera 80 for photographing the second detectors 16, 26, 36, and 46 is installed. A cylindrical lens may be installed in place of the polygon mirror 70, and the light from the laser light source 60 may be dispersed in the second detectors 16, 26, 36, and 46.
[0022]
In the microchip 1 of the present embodiment, the thickness of the substrate 2 is preferably 200 to 1000 μm, and more preferably about 400 to 500 μm. The thickness of the lid 3 is preferably 200 to 1000 μm, and more preferably about 400 to 500 μm.
The widths of the reaction chambers 13, 23, 33, 43, the first detectors 15, 25, 35, 45 and the second detectors 16, 26, 36, 46 are preferably 50 to 2000 μm, more preferably. 500 to 1000 μm. The depths of the reaction chambers 13, 23, 33, 43, the optical fiber detectors 15a, 25a, 35a, 45a and the second detectors 16, 26, 36, 46 are preferably 10-500 μm, more preferably 40 ~ 150 μm. The channel width other than the above is preferably 50 to 2000 μm, and more preferably 500 to 1000 μm. If the width and / or depth and / or the width of each channel is larger than the above range, the amount of sample solution and reagent solution required is increased, and the effect of microfabrication is reduced. Further, if the width and / or depth and / or the width of each flow path is smaller than the above range, the amount of solid particles stored is reduced, and the surface area of the reaction solid phase is reduced, which is not preferable.
[0023]
The slit widths of the upstream weir portions 12, 22, 32, 42 and the downstream weir portions 14, 24, 34, 44 need only be smaller than the diameter of the solid particles used as the reaction solid phase. Can be stopped.
The lengths of the upstream dam portions 12, 22, 32, 42 and the downstream dam portions 14, 24, 34, 44 are arbitrary, but are preferably 0.5 to 5 mm, and more preferably 1 to 2 mm. Is preferred.
[0024]
In order to allow the optical fiber plastic holders 15c, 25c, 35c, 45c to adhere to the lid 3 at the positions having the connecting portions 15a, 25a, 35a, 45a of the flow paths 10, 20, 30, 40. , Separated from each other by a predetermined distance or more. The separation distance is preferably about 0.5 to 10 mm, and more preferably about 1 to 5 mm.
In addition, a part of the second detection units 16, 26, 36, 46 of the flow paths 10, 20, 30, 40 is within the dispersion range of the laser light by the polygon mirror or the cylindrical lens, and the photographing range by the CCD camera. It is provided close to the inside. The proximity distance is preferably about 0.1 to 5 mm, and more preferably about 0.2 to 2 mm.
[0025]
The external shape of the microchip 1 of the present embodiment is, for example, a plate shape of 5.8 cm × 3.8 cm × thickness 1.0 mm, but the shape and dimensions are not limited to this, and are arbitrary. For example, it may be a flexible film shape (including a sheet shape, a ribbon shape, etc., the same applies below), a tube shape, and other complicated shapes. However, from the viewpoint of ease of molding, a plate shape is preferable.
[0026]
As the solid particles P used in the present invention, known ones can be used, but there are reagents for immobilizing on the solid particles a component to be measured in a sample solution or a complex in which components to be measured are complexed. The solid particle surface is preferably coated in advance. For example, it is preferable to use a surface on which a reaction reagent such as avidin is immobilized. The diameter of the solid particles is preferably 10 to 1000 μm, more preferably 10 to 100 μm. If it is larger than 1000 μm, the surface area as a reaction solid phase becomes insufficient, and if it is smaller than 10 μm, it becomes difficult to produce a dam part for damming solid particles, and the dam part can be sufficiently dammed. This is because the beads may flow out from.
[0027]
Next, the manufacturing method of the analyzer 1 of this embodiment is demonstrated.
First, sample liquid introducing portions 11, 21, 31, 41, and reaction chambers 13, 23, 33, and 43 of the flow channels 10, 20, 30, and 40 are formed on a silicon substrate 2 (for example, a thickness of 470 μm) by ICP etching. , First detectors 15, 25, 35, 45, second detectors 16, 26, 36, 46, sample outlets 17, 27, 37, 47, and grooves to be solid particle flow paths 50 (for example, width) 500 μm). Further, by ICP etching, the depth of the upstream weir portions 12, 22, 32, 42 and the downstream weir portions 14, 24, 34, 44 is the same as the depth of the reaction chamber along the flow direction. A slit is formed.
[0028]
On the other hand, a Pyrex glass lid 3 (for example, having a thickness of 500 μm) having the same planar shape as that of the substrate 2 is processed to have a hole penetrating from the front surface to the back surface. That is, when the substrate 2 and the lid 3 are laminated, the holes 11b, 21b, 31b, 41b are respectively provided at positions corresponding to the upstream ends 11a, 21a, 31a, 41a of the flow paths 10, 20, 30, 40, respectively. Process by sandblasting. Further, holes 17b, 27b, 37b and 47b are formed at positions corresponding to the downstream ends 17a, 27a, 37a and 47a, respectively. Further, holes 51b and 52b are formed at positions corresponding to 51a and the upstream end 52a in the downstream portion of the flow path 50a of the solid particle flow path 50, respectively. Further, holes 15b, 25b, 35b, and 45b are formed at positions corresponding to the first detectors 15a, 25a, 35a, and 45a, respectively.
Next, the glass fiber tube joints 11c, 21c, 31c, 41c, 17c, 27c, 37c, 47c, 51c, 52c are placed on the holes 11b, 21b, 31b, 41b, 17b, 27b, 37b, 47b, 51b, 52b, respectively. Join. Further, the plastic holders 25c, 25c, 35c, and 45c are joined to the holes 15b, 25b, 35b, and 45b, respectively.
[0029]
Finally, the substrate 2 and the lid 3 processed separately as described above are laminated and integrated by anodic bonding. As a result, liquid introduction paths communicating with the upstream ends 11a, 21a, 31a, and 41a of the flow paths 10, 20, 30, and 40 are formed. In addition, liquid discharge paths communicating with the downstream ends 17a, 27a, 37a, 47a of the flow paths 10, 20, 30, 40 are formed. Further, a solid particle introduction / discharge passage communicating with the downstream portion 51a of the solid particle passage 50a and the upstream end 52a of the 50a is formed. Moreover, an optical fiber can be connected to each of the first detectors 15a, 25a, 35a, 45a.
As described above, the microchip 1 included in the analyzer of the present embodiment is manufactured.
[0030]
Next, an analysis method using the analyzer of this embodiment will be described.
In the analysis method of the present embodiment, the measurement target component in the sample liquid or the complex obtained by complexing this is immobilized on the solid particles, the immobilized measurement target component or complex and the color reagent solution are reacted, The coloring reagent solution colored in this way is optically analyzed in a plurality of detectors arranged in parallel.
First, solid particles P to be a reaction solid phase are introduced from a solid particle introduction / discharge path and stored in reaction chambers 13, 23, 33, 43 in the microchip 1. At this time, the reaction chambers 13, 23, 33, 43 are provided with the upstream weir portions 12, 22, 32, 42 and the downstream weir portions 14, 24, 34, 44, respectively. Outflow from the reaction chamber can be prevented.
[0031]
Next, a sample solution, a reagent solution, and the like are introduced into the reaction chambers 13, 23, 33, and 43. For this, first, the carrier liquid is continuously introduced into the respective flow paths 10, 20, 30, 40 in the microchip 1 through the sample liquid introduction parts 11, 21, 31, 41. Next, the sample solution is introduced from the sample solution introduction parts 11, 21, 31, 41. As a result, the sample liquid is introduced into the reaction chambers 13, 23, 33, and 43 from the sample liquid introduction sections 11, 21, 31, and 41 through the upstream weir sections 12, 22, 32, and 42 together with the carrier liquid. Similarly, the reagent solution is introduced into the reaction chambers 13, 23, 33, and 43 together with the carrier solution.
[0032]
At this time, the order in which the sample solution and the reagent solution are introduced into the reaction chambers 13, 23, 33, and 43 may be arbitrary, but the order depends on the component to be measured in the sample and the reaction mechanism for the analysis. It is preferable. Alternatively, the sample solution and the reagent solution can be mixed in advance to obtain a sample reagent mixture, which can then be introduced into the reaction chambers 13, 23, 33, and 43. The reagent solution is selected according to the component to be measured in the sample and the reaction for the analysis, and may be one kind or plural kinds.
The sample solution and the reagent solution introduced into the reaction chambers 13, 23, 33, and 43 pass through the reaction chambers 13, 23, 33, and 43 as they are, and pass through the sample solution outflow portions 17, 27, 37, and 47, and are then used as waste liquid tanks. However, when a certain time is required for the reaction, the liquid is stopped from flowing for a predetermined time, or the reaction is performed by adjusting the flow rate of the carrier liquid.
[0033]
When the sample solution and the reagent solution are sequentially introduced, it is preferable to wash the reaction chambers 13, 23, 33, and 43 each time each reaction is completed. This is because the excess sample solution and reagent solution can be removed by washing, so that the reaction in the next stage can be prevented and quantitativeness can be ensured. As the cleaning liquid, pure water, phosphate buffer, Tris buffer, or the like is preferably used, but pure water is particularly preferably used.
[0034]
The component to be measured in the sample solution introduced into the reaction chambers 13, 23, 33, and 43 as described above reacts with a reagent that has been previously coated on the solid particles, and is immobilized on the solid particles. Alternatively, after immobilization, it subsequently reacts with the reagent in the reagent solution to form a complex on the solid particles. Alternatively, a complex is formed in a state where the sample solution and the reagent solution are mixed, and the complex is immobilized on the solid particles. Furthermore, after the reagent in the reagent solution is first immobilized on the solid particles, it may react with the component to be measured in the sample solution to form a complex on the solid particles. That is, by selecting a reaction suitable for the component to be measured in the sample liquid and using a reagent suitable for the reaction, the component to be measured or a complex obtained by combining the component is immobilized on the solid particles. .
[0035]
Next, the coloring reagent is introduced into the reaction chambers 13, 23, 33, 43 in the same manner as described above. The introduced coloring reagent reacts with the component to be measured immobilized on the solid particles or the complex obtained by complexing this, and the coloring reagent solution develops color. In this embodiment, the coloring reagent is introduced last. However, depending on the component to be measured, the coloring reagent may be introduced before the sample solution or other reagent solutions.
As described above, the component to be measured immobilized on the solid particles accommodated in the reaction chambers 13, 23, 33, and 43 or a complex obtained by combining the component to be reacted with the coloring reagent reacted to develop color. After the reagent solution has passed through the downstream weir portions 14, 24, 34, 44, the first detection portions 15, 25, 35, 45 and / or the second detection portions 16, 26, 36, 46 are subjected to optical analysis.
[0036]
In the analysis apparatus of this embodiment, an optical fiber, a laser light source, a CCD camera, and a polygon mirror or a cylindrical lens are installed as means for performing optical analysis. Therefore, various optical analyzes can be performed in the analyzer of this embodiment.
Specifically, the following optical analysis is mentioned, for example.
(1) A method in which a laser is introduced into an optical fiber and detected by the optical fiber.
(2) A method in which a laser is introduced into an optical fiber and detected by a CCD camera.
(3) A method in which light from a laser light source is scanned by a polygon mirror and detected by a CCD camera.
(4) A method in which light from a laser light source is scanned by a polygon mirror and detected by an optical fiber.
(5) A method in which light from a laser light source is dispersed by a cylindrical lens and detected by a CCD camera.
[0037]
【The invention's effect】
As described above, by performing analysis using the analyzer of the present invention, it is possible to easily and efficiently analyze a plurality of samples. That is, the measurement time can be shortened by having a plurality of parallel flow paths. In addition, by analyzing simultaneously in multiple detection units, there is no time difference in data acquisition, such as comparison of analysis results between standard substance and measurement sample, analysis of samples with different concentrations, etc., improving analysis accuracy Can be made.
Furthermore, the analyzer of the present invention, by the upper stream side dam portion and downstream dam portion is provided, the solid particles can be prevented from flowing out from the reaction chamber. In addition, if the upstream weir part and the downstream weir part have a slit-like structure that is substantially the same as the depth of the reaction chamber , liquids such as sample liquid, reagent liquid, and washing water stay on the bottom of the reaction chamber. Can be effectively prevented.
The analysis apparatus and analysis method of the present invention can be applied to various analyzes using solid particles as a reaction solid phase, and can be suitably used particularly for bioassays using immune reactions and the like.
[Brief description of the drawings]
FIG. 1 is a schematic plan view of a substrate portion of an embodiment of a microchip used in an analyzer according to the present invention.
FIG. 2 is a schematic plan view of a lid portion of the microchip shown in FIG.
FIG. 3 is a schematic diagram showing an example of an optical analysis means in the analysis apparatus of the present invention.
[Explanation of symbols]
1 .. Analyzing device 2 .. Substrate 3.. Lid 11, 21, 31, 41.. Sample solution introduction part 12, 22, 32, 42. Chambers 14, 24, 34, 44 ··· Downstream weirs 15, 25, 35, 45 ··· First detectors 16, 26, 36, 46 ··· Second detectors 17, 27, 37, 47 ··· Samples Liquid outlet

Claims (3)

A reaction chamber containing solid particles, a sample liquid introduction section upstream of the reaction chamber, a detection section downstream of the reaction chamber, and an upstream formed between the reaction chamber and the sample liquid introduction section An analyzer comprising a plurality of flow paths having a side weir part and a downstream weir part formed between the reaction chamber and the detection part,
The upstream dam portion and the downstream dam portion of the plurality of flow paths are dam portions for damming the solid particles and allowing liquid to pass through , respectively.
And the detection part of these flow paths has the 1st detection part to which each optical fiber is connected, and the 2nd detection part photoed with a CCD camera,
The first detectors are formed in parallel and spaced apart from each other by more than a distance that allows optical fibers to be connected to each other, and the second detectors are mutually within a distance that enables photographing by a CCD camera. An analyzer characterized by being formed in close proximity and in parallel . The upstream weir portion and the downstream weir portion of the plurality of flow paths each have a slit-like shape formed in the flow direction with a width narrower than the particle diameter of the solid particles and substantially the same depth as the reaction chamber. The analyzer according to claim 1 which is a weir part. It is an analysis method using the analyzer of Claim 1 or 2 , Comprising: The measurement object component in a sample liquid or the composite_body | complex which compounded this was fix | immobilized to the said solid particle, and this measurement object component or composite_body | complex fixed. And a coloring reagent solution, and the coloring reagent solution colored by this is subjected to photoanalysis in a plurality of detection units arranged in parallel.

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