JP3513475B2 - Hair growth / hair restorer - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a hair-growth / hair-growth agent which has remarkable hair-growth and hair-growth effects and which does not cause significant side effects on the living body.

[0002]

2. Description of the Related Art Conventionally, various researches and developments have been conducted on hair growth and hair growth agents, such as hormone agents, blood flow promoters,
Cellular activator components and the like have been provided, but most of them have a problem that they have side effects even if they have excellent hair growth and hair growth effects.

Therefore, in order to achieve hair growth and hair growth effects and improve safety, hair growth and hair growth agents have been proposed in which active ingredients present in natural plants are extracted instead of chemical compounds. For example, in Japanese Patent No. 2564226,
A hair-growth / hair-growth agent containing one or more plant extracts of the genus Ebine or the genus Orchidaceae as an active ingredient has been proposed.

[0004]

However, the conventional plant extract components are safe, but have a drawback that the hair-growth and hair-growth effects are not sufficient, and the hair-growth and hair-growth effects are excellent, and the safety is high. At present, no excellent active ingredient has been found.

In view of the above, it is an object of the present invention to provide a hair growth / hair growth agent that exhibits an effective hair growth / hair growth effect without causing side effects.

[0006]

[Means for Solving the Problems] As a result of earnest research to achieve the above object, the present inventor found that an extract of a plant of the genus Cymbidium of the family Orchidaceae has excellent hair growth / hair-growth effects and may not cause side effects. That is, the present invention has been completed and the present invention has been completed.

The genus Cymbidium is a monocotyledon of the Orchid family, about 70 species of which are distributed mainly in the subtropical to tropical regions of Asia, and has a characteristic pseudocorm. There are an extremely large number of Cymbidium plants, including those that have undergone variety registration under the Seed and Seedling Act.
Sulgalan (Cym.ensifolium), Kaoran (Cym.faberi), Orchid flower (Cym.flor)
ibundum), Tiger head orchid (Cym. ookeria)
num), yellow cicada orchids (Cym. iridiodes), kanran (Cym. kanran), rabbit ear orchids (Cym. l)
ancifolium), hard-leaf hanging orchid (Cym. pendu)
lum), Housairan (Cym.sinense),
Great Flower Marie Laurentin (Cym.Gr
eat Flower'Marie Laurenc
in '), Princess Diana (Cym. Prince)
ss Diana), Princess Masako (Cym.Pr)
incess Masako, Princess Nobuko (C
ym. Prince Nobuko, Rose Wine, New Century (Cym. Rose Wine'Shin)
seiki '), Great Flower Ballerina (Cy
m. Great Flower 'Ballerin
a), Great Catty Mariko (Cym. Great
Katy'Mariko '), Gentile Peppermint (Cym. Jenteel'Pepper
mint), Lucky Gloria Aguri (Cym.Lu
cky Gloria'Aguri '), Yuho Color (Cym.'UFO Color'), Lucky Gloria, God of Fortune (Cym. Lucky Gloria)
‘Fukunokami’, Seaside Saya (C
ym. Seaside'Saya '), raspberry
Miroir (Cym. Raspberry 'Milo
ile '), Satin Doll Golden Yellow (Cy
m. Satin Doll'Golden Yell
ow '), Great Catty Little Laurentin (C
ym. Great Katty'LittleLaur
Encin '), Great Catty Hatsukoi (Cym.G
read Katy'Hatsukoi '), Great Catty Art Nouveau (Cym. Great K)
atty'Art Nouveau ', Happy Face Maki Special (Cym. Happy Fac)
e'Maki Special ') and the like are known. Some of them are used in China as a medicine for respiratory diseases such as antitussive, a herbal medicine for improving blood circulation, and an anthelmintic herb.

[0008] The present inventor has conducted extensive studies on Cymbidium plants for many years, and as a result, particularly the extract of pseudo-bulbs (bulbs) shows good hair growth and hair growth effects and is safe without side effects. I found out that.

That is, the hair growth / hair growth agent according to the present invention is
As an active ingredient, it is characterized by containing an extract obtained from a pseudo-corm of a plant of the genus Cymbidium of the family Orchidaceae.

As described above, there are a great number of plants belonging to the genus Cymbidium of the family Orchidaceae, but among them, the varieties registered name by the seed and seedling method, the pseudo-corm of Great Flower Marie Laurentin, is excellent in hair growth and hair growth effects.

The blending amount of the extract is 0.2 to 3
It is preferably blended in an amount of 0% by weight, preferably 0.5 to 10% by weight. When the content of the extracted extract was less than 0.2% by weight, or when the content of the extracted extract was more than 30% by weight, neither preferable hair growth / hair growth effect was obtained. A particularly preferable blending amount is 3 to 6% by weight, and among them, the blending amount of 5% by weight showed the most excellent hair growth and hair growth effect.

For the extraction of the extract, either a physical extraction method in which the pseudo-corms are finely sliced and then crushed by a crusher or a solvent extraction method can be employed. As the extraction solvent, water, ethanol, propylene glycol, 1,3-
Hydrophilic solvents such as butylene glycol are mentioned, and these may be used as a mixture of two or more kinds. In the case of the ethanol extraction method, it is possible to employ a method in which pseudo corms are cut into small pieces, crushed with a crusher, immersed in an aqueous ethanol solution, subjected to extraction operation with occasional stirring, and impurities are removed by filtration.
For example, 30 g of pseudo-corm is treated with 60% ethanol aqueous solution 10
When it is immersed in 0 g and extracted for 14 days with occasional stirring and impurities are removed by filtration, a 50% alcohol solution containing about 20% by weight of the extracted extract can be prepared.

In the case of the hot water extraction method, 100 ml of purified water was added to 5 g of the pulverized product obtained in the same manner as above, and the mixture was extracted by heating on a boiling water bath for 2 hours, and then an equal amount of 95% ethanol was added, About 20% if the precipitate is removed with filter paper after standing still
A 50% alcoholic solution containing the extract of can be prepared.

[0014]

[Experimental Example 1] Hair growth and hair growth tests were carried out in mice using the extract of pseudo-corm of Cymbidium plant. The extract of pseudo-corm is Greatflower Mary Laurentin (Cultivar Registration No. 2841
5% by weight and 10% by weight of cymbidium tincture were prepared according to the Japanese Pharmacopoeia, "Tincture agent" section of the Japanese Pharmacopoeia. As the mouse, a total of 36 C ₃ H mice (CLEA Japan, Inc.), 57 days old, and 9 male C ₃ H / HeNCr mice weighing 23 to 28 g were used. The hair of 57-day-old mice is in the resting period of hair growth.

After the hair on the back of the mouse was clipped with a hair clipper, hair was removed with a depilatory cream, and from 61 days of age, 100 μl each of 5% by weight cymbidium tincture, 10% by weight cymbidium tincture and Reup (Taisho Pharmaceutical Co., Ltd.) was applied daily to the hair removal part. 1
It was applied once and evenly. Reap was used as a comparative drug because it has been found to have high hair growth and hair growth effects.
Photographs were taken at 1st week, 2nd week and 3rd week after the start of application, and the density of the hair-growth portion was quantified by image processing for comparative examination. For reference, photographs of the 5% by weight cymbidium tincture, the reup, and the control group taken every week are attached as FIGS. 1, 2, and 3. The first, second, and third weeks are shown in order from the left. 5 in the control group
Only 0% ethanol solution was applied. The test results are shown in the following table.

The numerical values of ± α in the table represent variations in color among individual mice in each group, that is, variations in hair density.

[0017]

[Table 1]

As is clear from the comparison with the control group in Table 1, both 5% by weight and 10% by weight of the cymbidium tincture exhibited excellent hair growth and hair growth effects. 5% by weight
It was found that the effect of Cymbidium tincture was more remarkable than that of 10% by weight of Cymbidium tincture, and the effect was almost the same as that of the control drug, Reup.

[0019]

[Experimental Example 2] A skin sensitization test was conducted using guinea pigs in order to evaluate the safety of an extract (hereinafter referred to as OCBE) of a pseudo-bulb of a Cymbidium plant. As a guinea pig, a 4-week-old Hartley male guinea pig (Japan SLC), which is widely used as an animal model for skin toxicity test, was preliminarily bred for 1 week, and a guinea pig in good general condition was used for the test. The number of animals in one group was 6. Throughout the preliminary breeding period and the test period, constant conditions, room temperature 22 ± 2 ° C., humidity 60 ± 10%,
F in the breeding room set to 12 hours lighting (8 to 12)
Two cages were housed in each cage made of RP resin, and solid feed (RC-4, Oriental yeast) and tap water were freely ingested. The body weight of the animals is 310 at the start of the test.
338 g, and 461-545 g at the time of induction treatment.

As the test substance, two kinds of extracts of pseudo-bulbs of the genus Orchidaceae Cymbidium (Orchidaceae Cymbidium) with different extraction methods, that is, ethanol extract (hereinafter referred to as OCBE-E) and hot water extraction are used. Using an extract (hereinafter referred to as OCBE-W), each skin primary irritation was examined. OCBE-E is obtained by slicing pseudo-corms into small pieces, crushing with a crusher, and freeze-drying to obtain 5 g of a dried product, to which 100 ml of 50% ethanol aqueous solution was added and extracted at room temperature for 7 days. It is a 50% ethanol solution containing 100% of the extract.

OCBE-W was prepared by adding 100 ml of purified water to 5 g of the dried product obtained in the same manner as described above, and adding 2 on a boiling water bath.
After extraction by heating for an hour, an equal amount of 95% ethanol was added, the mixture was allowed to stand, and the precipitate was removed with filter paper.
It is a 50% ethanol solution containing the extract of.

As a positive control drug, DNCB (dini
(Trochrobenzene, Wako Pure Chemical Industries, Ltd.) was used at a concentration that showed clear sensitization and did not show irritation in the induction treatment. DN of 0.1% for intradermal injection, 1.0% for application and 0.01% for sensitization treatment.
CB was prepared, and each was adjusted with ethanol (99.5%, Wako Pure Chemical Industries) before use. In addition, the control group included
Only 50% ethanol solution was used.

The skin sensitization test is performed by maximiza.
Tion test (Magnusson B, Kli
gman A: The identification of contact allerg
ensby animal assay; The guinea pig maximization te
st-J. Invest Dermatol 52:26.
8-276, 1969).

[Sensitization method] The shoulder blade was shaved with an electric shaver to a size of 2 cm x 4 cm, and three types of samples were prepared. (1) Distilled water and FCA
(Freund's complete adujuv
ant, Difco) 1: 1 water-in-oil (W / O) emulsion, (2) test substance and positive control drug, (3) 1: 1 emulsion of test substance and positive control drug and FCA Symmetrically, 0.05 ml was intradermally injected. One week later, 10% sodium lauryl sulfate (mixed with Vaseline base) was applied to the site of intradermal injection, and the next day, 0.1 ml of the test substance and the positive control drug were closed and applied to the same site for 48 hours. [Induction method] Induction was performed 2 weeks after the closed application. One side 2 cm x 4 cm of the flank was shaved with an electric shaver, and a test substance and 0.1 ml of the positive control drug were closed and applied for 24 hours using a patch test plaster (regular size, Torii Pharmaceutical Co., Ltd.).

[Observation] From the 24th to the 96th hour after the treatment, every 24 hours, the evaluation method of Draise (Draise J: Dermal toxity, Apprisal o shown in Table 2) was used.
f the safetyof chemicals in foods, drugs and cosme
erythema and edema were observed according to tics).

[0026]

[Table 2]

As a result, for each test solution, the number of guinea pigs showing a positive reaction is shown in Table 3.

[0028]

[Table 3]

In the positive control drug DNCB group, positive reaction was observed in all cases as shown in Table 3. Specifically, 24 after treatment
From the time point, erythema with a clear boundary was observed in 5 cases, and moderate erythema with mild edema was observed in 1 case. After 48 hours, 3 cases of erythema were intensified and became moderate erythema with mild edema. After that, erythema was alleviated after 72 hours in all cases, and in 4 cases, scales were observed on the surface at 72 hours and desquamation occurred at 96 hours. In all of the OCBE-W group and the OCBE-E group, no change was observed in the skin from 24 to 96 hours after the treatment, as in the control group using only the 50% ethanol aqueous solution.

As is clear from the above results, OCBE
-E group and OCBE-W group showed no positive reaction in the skin sensitization test, and DNCB group in the positive control test showed erythema in all 6 cases. Therefore, the results of Maurer et al. T, Thomann P, Wei
rich E, Hess R, Contact Der
Matitis 4: 321-333, 1978), indicating that the study is well established. Therefore, regarding skin sensitization, OCBE-E
It was confirmed that the safety was confirmed in both OCBE-W and OCBE-W.

[0031]

[Experimental Example 3] A clinical test was conducted for 6 months on 20 males and females (30 to 60 years old) randomly selected from various alopecia patients.

The test was carried out by crushing the pseudo-corm of Cymbidium plant into fine pieces, crushing it with a crusher, and using a ethanol extraction method to obtain a 20% by weight extract of 50% alcoholic solution, specifically Greatflower Marie Laurent. 20% by weight of pseudo corms, using pseudo corms of Sun (variety registration 2841)
Extracted extract containing 50% alcohol solution 25.0% by weight, ethyl alcohol 40.0% by weight, L-menthol 0.1% by weight, 1.3 butylene glycol 2.0% by weight, ginger tincture 0.1% by weight %, Purified water 32.8
A hair-feeding tonic was prepared by blending it in a weight percentage.

As a test method, the above-mentioned hair tonic was sprayed and applied to the head in a usual use mode, massaged well, and this was instructed to be performed once or twice daily.

For each of the three items of "promotion of hair growth", "prevention of hair loss", and "prevention of dandruff", judgment was made in four stages of "significant effect", "effective", "somewhat effective" and "no effect". The results are shown in Table 4.

[0035]

[Table 4]

For comparison, all leaves (leaves / stems / roots) of a cattleya plant of the family Orchidaceae are shredded, crushed with a crusher, and obtained by the ethanol extraction method in the same manner as described above. A 50% alcoholic extract solution of 20% by weight of the extract was prepared, and tested using a hair-reducing tonic prepared in the same manner except that the extract of pseudocorms of Great Flower Marie Laurentin was replaced with the extract of Cattleya plants. As a result, the results shown in Table 5 were obtained. The Cattleya plants include hybrids obtained by crossing Cattleya genus, Cattleya genus with Lelia genus, Brasabora genus and Sophronitis genus.

[0037]

[Table 5]

As is clear from the results shown in Tables 4 and 5, the composition containing the pseudo-corm extract of the Cymbidium plant showed that
Although it was confirmed that it is effective in preventing both hair loss and dandruff, the orchidaceous plant and the extract extracted from the cattleya plant did not give very favorable results. In addition, a skin sensitization test was conducted on the extract extracted from the cattleya plant in the same manner as in Experimental Example 2.
A positive reaction was seen for the example. In addition, when a human batch test (judgment after 48-hour closed test) was performed for skin irritation, a positive reaction was observed in 3 out of 10 people. Therefore, it was found that the extract extracted from the Cattleya plant cannot be expected to have the same effects as those found in the pseudo-corm extract extracted from the plant of the genus Cymbidium, and there is a problem in safety.

Further, using Great Flower Marie Laurentan, which is highly effective for Cymbidium plants, the parts other than the pseudo corms, that is, the mixture of leaves and stems, are shredded and crushed with a crusher, and the same procedure as above is performed. An extract extracted by the ethanol extraction method was prepared and tested using a hair-growth tonic prepared with the same composition except that it was replaced with the extract extracted from the pseudoflower of Great Flower Marie Laurentin. It was confirmed that the use of the pseudo-corm portion was particularly effective, because the preferable results were not obtained in both prevention and dandruff prevention.

[0040]

Examples Therefore, as a specific example of the hair-growth and hair-growth agent according to the present invention, a pseudo-corm of a Cymbidium plant, preferably Great Flower Marie Laurentin (Variety Registration No. 2
841) pseudo-corm extract 5.0% by weight, ethanol 50.0% by weight, L-menthol 0.1% by weight,
A hair growth / foster agent mixed with 2.0% by weight of 1,3 butylene glycol, 0.1% by weight of ginger tincture and 42.8% by weight of purified water is recommended from the viewpoint of efficacy and safety. However, the compounding amount is not necessarily limited to this amount, and the pseudo-corm extract of cymbidium can be used in the range of 0.2 to 30% by weight, preferably 0.5 to 10% by weight. The amount of menthol, 1,3 butylene glycol, and ginger tincture can be adjusted within the range usually used as a hair growth / hair growth agent.

[0041]

EFFECTS OF THE INVENTION As is clear from the above description, the hair growth and hair-restoring agent according to the present invention contains, as an active ingredient, an extract of pseudo-corm of a plant belonging to the genus Cymbidium of the family Orchidaceae, preferably Great Flower Marie Laurentan (registered variety). (No. 2841)
By including the pseudo-corm extract of the above, it was possible to achieve a hair growth and hair growth effect that was far superior to other plants belonging to the Orchidaceae family. Have In addition, since it can be expected to prevent hair loss and dandruff, this hair growth and hair restoration agent
It is also useful for maintaining healthy scalp and hair.

[Brief description of drawings]

FIG. 1 is a graph showing a hair growth / hair growth test using a mouse, after applying the 5% cymbidium tincture according to the present invention to the hair loss part.
Photos taken each week over the week.

FIG. 2 is a photograph taken every week for 3 weeks after the application of Reup as a comparative drug.

FIG. 3 is a photograph of a control group similarly coated only with a 50% ethanol solution, taken every week for 3 weeks.

─────────────────────────────────────────────────── ─── Continuation of the front page (58) Fields surveyed (Int.Cl. ⁷ , DB name) A61K ^7/ 00-7/50 A61K 35/78

Claims (4)

(57) [Claims] 1. A hair-growing / hair-growing agent containing, as an active ingredient, an extract obtained from a pseudo-corm of Great Flower Marie Laurentin, a plant belonging to the genus Cymbidium of the family Orchidaceae. 2. The hair-growth / hair-growth agent according to claim 1, which contains 0.2 to 30% by weight of the extracted extract. 3. The hair-growth / hair-growth agent according to claim 1, wherein the extract is contained in an amount of 0.5 to 10% by weight. 4. The hair-growth / hair-growth agent according to claim 1, which contains 5% by weight of the extracted extract.