JP3115553U - Medium storage device, petri dish lid - Google Patents

Abstract

[PROBLEMS] To provide a culture solution storage device capable of suppressing wasteful use of a culture solution in a petri dish for culturing microorganisms or living organisms.
The culture solution C is placed in the culture solution storage recess 66 for about the sixth minute, that is, until the water level of the hoses 13 and 15 is higher than one end, and the living cells D are placed in the culture solution C. . The amount of the culture solution C stored in the culture solution storage recess 66 is about 2.4 cc. On the other hand, the amount of the culture solution C when storing the culture solution C directly in the petri dish body 19 is about 41 cc. Therefore, the amount of the culture medium C can be reduced to about 1/17, the amount of the culture medium C can be greatly reduced, and the water level necessary for culturing the living cells D can be obtained. Can do.
[Selection] Figure 4

Description

  The present invention relates to a petri dish used for culturing microorganisms and living cells, a culture solution storage device contained in the petri dish, and a lid of the petri dish.

  When culturing microorganisms or living cells, a petri dish having a circular bottom is widely used. This petri dish is comprised by the main body and the lid | cover used as needed. A commercially available petri dish body widely used for culturing microorganisms has an inner diameter of 35 mm and a depth of 9 mm. A culture solution is stored in the petri dish body, and microorganisms and living cells are cultured in the culture solution.

However, only a very small amount of the culture solution stored in the petri dish is consumed for cultivation, and most of it is put in order to keep the water level above a certain level. There is a problem.
Some types of culture broth are extremely expensive, and useless use of the culture broth as described above is a problem that cannot be ignored on the experimental cost.
Further, in the so-called substitution culture in which the culture solution stored in the petri dish body is replaced, the culture solution is particularly wasted.
The present invention has been made paying attention to the above-described conventional problems, and an object thereof is to provide a culture solution storage device and a petri dish lid capable of suppressing wasteful use of the culture solution.

  The present invention has been made to solve the above-mentioned problems, and the device according to claim 1 is an instrument main body installed and accommodated on the bottom surface of the petri dish main body, and a culture solution storage formed on the instrument main body. It is a culture solution storage instrument characterized by comprising a hole or a recess.

  The invention of claim 2 is the culture solution storage device according to claim 1, wherein at least two hoses having one end communicating with the hole or the recess are provided. .

  The device of claim 3 is the culture solution storage device according to claim 1 or 2, wherein the device body is made of an elastically deformable material.

  The invention of claim 4 is the culture solution storage device according to claim 3, wherein a suction recess is formed on the bottom surface of the device body.

  The device of claim 5 is the culture solution storage device according to any one of claims 1 to 4, wherein the device body is made of a material that can see through the inside of the hole or the recess from the outside. It is a storage device.

  The invention of claim 6 is the culture solution storage device according to any one of claims 1 to 5, wherein the device main body has a diameter slightly smaller than the inner diameter of the petri dish main body having a bottom surface in which the device main body is accommodated. A culture medium storage device characterized by being formed in a disc shape.

  The invention of claim 7 is the culture solution storage device according to any one of claims 1 to 6, wherein the hole or the recess is provided in a central portion of the device body. is there.

  The invention of claim 8 is the culture solution storage device according to any one of claims 2 to 7, wherein one end communicating with the hole or recess of at least one hose is communicated with the hole or recess of another hose. It is the culture solution storage instrument characterized by having arrange | positioned in the high place from the one end part which performs.

  The invention of claim 9 is a lid for a petri dish that covers the upper surface opening of the petri dish main body in which the instrument main body of the apparatus for storing a culture solution according to any one of claims 2 to 8 is accommodated. The petri dish lid is provided with a drawer opening.

  The invention of claim 10 is the petri dish lid according to claim 9, wherein the outlet is formed by a notch continuous from the upper surface portion to the peripheral surface portion of the petri dish lid. .

  An eleventh aspect of the present invention is the petri dish lid according to the ninth or tenth aspect, characterized in that a cover portion is provided above the drawer opening and covering the upper surface of the drawer opening. .

  A twelfth aspect of the invention is a petri dish comprising the petri dish lid according to any one of the ninth to eleventh aspects and a petri dish body whose upper surface opening is covered by the petri dish lid.

According to the culture solution storage device of the present invention, it is possible to suppress wasteful use of the culture solution.
According to the petri dish and the petri dish lid of the present invention, the hose communicating with the hole or the recess of the culture medium storage device can be pulled out of the petri dish.

The petri dish 2, the culture solution storage device 1, and the petri dish lid 3 according to the first embodiment of the present invention will be described with reference to FIGS.
The structure of the culture solution storage device 1 will be described.
Reference numeral 5 denotes an instrument body. The instrument body 5 is made of transparent silicone and can be elastically deformed. The instrument body 5 has a disk shape having a diameter P of 33 mm and a thickness T of 5 mm, which is slightly smaller than the inner diameter of the petri dish body 19 to be described later, and a round hole 6 for storing a culture medium having a diameter of 8 mm is formed at the center. Further, as shown in FIG. 2, a suction recess 7 is provided on the bottom surface of the instrument body 5.

  A pair of holding slits 9 and 11 are formed in the instrument body 5, and hoses 13 and 15 are held in the holding slits 9 and 11. One end of the hose 13, 15 is provided in a state of being exposed on the peripheral surface of the hole 6, and communicates with the hole 6. One ends of the hoses 13 and 15 are arranged to face each other slightly below the center of the hole 6 in the height direction. The hoses 13 and 15 have a length of about 30 cm, and connecting pipes 17 are attached to the other ends.

The structure of the petri dish lid 3 will be described.
The lid 3 covers the top opening of the petri dish body 19, and the petri dish 2 is configured by the petri dish body 19 and the lid 3. The petri dish body 19 has a bottom surface portion 65 formed in a circular shape in plan view, an inner diameter P2 of 35 mm, and a depth T2 of 9 mm. The petri dish 2 is made of transparent plastic.
The lid 3 is formed with a pair of notches 21, 23 that are continuous from the upper surface portion 20 to the peripheral surface portion 22, and the notches 21, 23 are arranged to face each other. The notches 21 and 23 are open at the lower end of the peripheral surface portion 22.

Further, cover housings 25 and 27 as cover portions are provided on the upper surface portion 20 of the lid 3, and the cover housings 25 and 27 are formed in a shape in which portions other than the opening portions 29 and 31 opening outward are closed. (See FIG. 3). Accordingly, the cover housings 25 and 27 cover the upper surfaces of the notches 21 and 23. Therefore, falling bacteria can be prevented from entering the petri dish body 19.
Note that three convex portions 4 are formed on the inner surface of the lid 3.

A microscopic observation incubator 33 in which the petri dish 2 is housed will be described.
The microscopic observation incubator 33 includes a heating plate 35, a water tank unit 47 whose upper and lower surfaces are open, a lid 39, and the like.
The heating plate 35 includes a housing 41 and a heater 43 held by the housing. A round hole 45 is formed at the center of the heater 43.

The water tank unit 47 is provided with an annular water tank 49. One end of the water supply pipe 51 is provided so as to protrude into the water tank 49, and a water supply hose 53 is connected to the other end of the water supply pipe 51. One end of the gas supply pipe 55 is also provided so as to protrude into the water tank 49, and a gas hose 57 is connected to the other end of the gas supply pipe 55.
The water tank unit 47 is provided with a pair of pressing plates 58 made of thin metal plates, and one end of the pressing plate 58 is rotatably supported by a block 61 fixed to the water tank 49. .
The lid 39 includes a housing 59 and a transparent glass heater 61 held by the housing 59.

Next, a procedure for accommodating the petri dish 2 containing the culture storage tool 1 in the microscope observation incubator 33 and culturing living cells and the like will be described.
The heating plate 35 is fitted into the opening Sa of the microscope stage S, and the stepped portion of the housing 41 is locked to the peripheral edge of the opening Sa. A water tank unit 47 is installed on the stage S so as to correspond to the heating plate 35, and a lid 39 is provided so as to cover the upper surface opening of the water tank unit 47.

A controller (not shown) is operated to turn on the heater 43 of the heating plate 35 and the transparent glass heater 61 of the lid 39. Further, water is supplied to the water tank 49 through the water supply hose 53 and the water supply pipe 51 and stored. Further, CO ₂ gas is supplied into the microscopic observation incubator 33 through the gas hose 57 and the gas supply pipe 55.
The heater 43, the glass heater 61, and the like are controlled based on the detection results of the temperature sensor and the like provided in the microscope observation incubator 33, and the temperature in the microscope observation incubator 33 is maintained at a predetermined condition.

On the other hand, the instrument body 5 of the culture solution storage instrument 1 is accommodated in the petri dish body 19. As described above, since the diameter of the instrument main body 5 is set to be slightly smaller than the inner diameter of the petri dish main body 19, the central part of the instrument main body 5 and the petri dish main body can be simply accommodated in the petri dish main body 19. The center part of 19 overlaps.
Further, since the recess 7 is formed on the bottom surface of the instrument body 5, if the instrument body 5 is pressed against the bottom surface portion 65 of the petri dish body 19 to be elastically deformed and the air in the recess 7 is discharged, the recess 7 Becomes a negative pressure, and the instrument body 5 is adsorbed and fixed to the bottom surface portion 65 of the petri dish body 19.

  Thereby, a culture solution storage recess 66 is formed by the round hole 6 of the instrument body 5 and the upper surface of the bottom surface portion 65 of the petri dish body 19. The culture solution C is put into the culture solution storage recess 66 for about the sixth minute, that is, until the water level rises from one end of the hoses 13 and 15, and the living cells D are put into the culture solution C. The amount of the culture solution C stored in the culture solution storage recess 66 is about 2.4 cc. On the other hand, the amount of the culture solution C when storing the culture solution C directly in the petri dish body 19 is about 41 cc. Accordingly, the amount of the culture medium C used can be reduced to about 1/17, the amount of the culture medium C used can be significantly reduced, and the water level necessary for culturing the living cells D can be obtained. it can.

  Then, the hoses 13 and 15 are pulled out in the left and right directions, and the other end portions of the hoses 13 and 15 are arranged outside the petri dish body 19. Next, the lid 3 is covered to cover the upper surface opening of the main body 19. At this time, the notches 21 and 23 are made to correspond to the hoses 13 and 15 arranged outside the petri dish body 19. As a result, the hoses 13 and 15 are pulled out from the notches 21 and 23 and the openings 29 and 31 of the cover housings 25 and 27. Therefore, the hoses 13 and 15 can be prevented from being crushed by being sandwiched between the edge of the petri dish body 19 and the lid 3.

Thus, the notches 21 and 23 are formed continuously from the upper surface portion 20 to the peripheral surface portion 22 of the lid 3, and the cover housings 25 and 27 are provided with openings 29 and 31 that open outward. The hoses 13 and 15 can be pulled out of the petri dish 2 simply by covering the petri dish body 19 with the lid 3.
In addition, since the three convex portions 4 are formed on the inner surface of the lid 3, the convex portions 4 abut on the upper end portion of the petri dish body 19. Accordingly, a gap is formed between the upper end portion of the petri dish body 19 and the inner surface of the lid 3, and the air permeability of the petri dish 2 is maintained even when the petri dish body 19 is covered with the lid 3.

The petri dish 2 is accommodated in a microscopic observation incubator 33. That is, the petri dish 2 is installed on the heater 43 and is positioned so as to correspond to the center of the culture solution storage recess 66 and the round hole 45 of the heater 43. Since the central part of the instrument body 5 and the central part of the petri dish body 19 overlap each other as described above, if the center part of the petri dish body 19 is matched with the central part of the round hole 45, the culture is performed. The liquid storage recess 66 and the central portion of the round hole 45 are overlapped.
Then, the petri dish 2 is fixed by elastically contacting the pair of pressing plates 58 with the lid 3.
Since the petri dish 2 and the instrument body 5 are made of a material that can be seen through, the state of the culture solution C and the living cells D in the culture solution storage recess 66 is external even when stored in the incubator 33 for microscope observation. Can be clearly seen.

  Further, the hoses 13 and 15 are pulled out from the hose pull-out hole 67 of the microscope observation incubator 33, and a culture solution suction device is connected to the connection pipe 17 of the hose 13 via the hose 69. A culture medium supply device is connected to the pipe 17 via a hose 69. Then, the culture solution C in the culture solution storage recess 66 is sucked through the hose 13, the pipe 17 and the hose 69, and is cultured into the culture solution storage recess 66 through the hose 15, the connection pipe 17 and the hose 69. Solution C is supplied and so-called substitution culture is performed.

  As described above, the temperature, humidity, and the like in the microscopic observation incubator 33 are maintained at predetermined conditions, and the convex portion 4 is formed on the upper end portion of the petri dish body 19 even when the petri dish body 19 is covered with the lid 3. By contacting, there is a gap between the upper end portion of the petri dish body 19 and the inner surface of the lid 3, so that the inside of the petri dish 2 also has environmental conditions suitable for culturing living cells D.

  The living cells D are cultured in the petri dish 2 accommodated in the microscope observation incubator 33 as described above, and the state change is observed with a microscope. That is, light is radiated from the condenser E located above the microscope observation incubator 33 to the living cells D in the culture medium storage recess 66, and the microscope observation incubator 33 and the round hole Sa of the stage S are sandwiched therebetween. A biological cell D is captured by an objective lens F provided to face the lower side of the condenser E, and is observed with a microscope.

A culture solution storage device 71 according to Embodiment 2 of the present invention will be described with reference to FIGS.
The culture solution storage device 71 has substantially the same structure as the culture solution storage device 1 according to the first embodiment, and the method of use thereof is also the same. Are denoted by the same reference numerals used in the first embodiment, and the description thereof is omitted.
A recess 77 having a bottom 75 is formed at the center of the instrument body 73 of the culture solution storage instrument 71. The instrument main body 73 and the bottom 75 are made of transparent silicone. The instrument body 73 has a diameter P of 8 mm, a thickness T of 5 mm, and a bottom 75 having a thickness of 0.5 mm.

A culture solution storage device 81 according to Embodiment 3 of the present invention will be described with reference to FIGS.
The culture solution storage device 81 has substantially the same structure as the culture solution storage device 1 according to the first embodiment, and the usage method is the same. Therefore, only the difference will be described, and the same structural portion will be described. Are denoted by the same reference numerals used in the first embodiment, and the description thereof is omitted.
One end portion communicating with the hole 6 of the hose 13 is disposed at a higher position than the one end portion communicating with the hole 6 of the hose 15.

As shown in FIG. 10, the culture solution storage device 81 is accommodated in the petri dish body 19 and the culture solution C is stored in the culture solution storage recess 66. A culture medium suction device is connected to the hose 13 as in the first embodiment, and a culture liquid supply device is connected to the hose 15.
The culture solution C in the culture solution storage recess 66 is sucked through the hose 13 and the culture solution C is supplied into the culture solution storage recess 66 through the hose 15 to perform so-called substitution culture. The water of the culture solution C stored in the culture solution storage recess 66 needs to be kept constant.

  The ability of the culture solution suction device to suck the culture solution C in the culture solution storage recess 66 is set to be higher than the ability of the culture solution supply device to supply the culture solution C into the culture solution storage recess 66. The culture solution C in the culture solution storage recess 66 is sucked through the hose 13, but when the water level of the culture solution C is below the opening at one end of the hose 13, the culture solution suction device is driven. However, the culture medium C is not sucked any more. That is, when the culture solution C is supplied from the hose 15 to the culture solution storage recess 66, the culture solution C in the culture solution storage recess 66 increases and the water level rises above the lower end of the one end opening of the hose 13. Only the culture medium C is sucked from the hose 13.

As described above, the ability of the culture solution suction device to suck the culture solution C in the culture solution storage recess 66 is higher than the ability of the culture solution supply device to supply the culture solution C into the culture solution storage recess 66. Since it is set, the supply amount of the culture medium C does not exceed the suction amount. Therefore, the water level of the culture solution C stored in the culture solution storage recess 66 is always adjusted to be substantially the same height as the lower end of the one end opening of the hose 13.
As described above, by using the culture solution storage device 81 according to the third embodiment, the water level of the culture solution C in the culture solution storage recess 66 can be easily and reliably managed.

The embodiment of the present invention has been described in detail above, but the specific configuration is not limited to this embodiment, and the present invention can be devised even if there is a design change without departing from the gist of the present invention. included.
For example, it is good also as a structure which does not provide the slits 9 and 11 and the hoses 13 and 15 in the instrument main bodies 5, 73 and 81. In this configuration, it is not used for so-called substitution culture.
Moreover, although the instrument main body 5 was comprised with the material which can see through the whole, it is not limited to this, You may comprise with the material which is not transparent. Moreover, the instrument main body 77 should just be comprised with the material which can see through at least the bottom part 75, and may comprise it with the material which cannot see through other parts.
Furthermore, the instrument bodies 5, 73, 81 may be made of, for example, hard plastic, glass, or the like that does not elastically deform. Three or more hoses 13 and 15 may be provided.

Moreover, it is good also as a structure which does not form the recessed part 7 in the bottom face of the instrument main body 5,73,81.
The diameters and thickness dimensions of the instrument bodies 5, 73 and 81 and the diameters and depth dimensions of the round holes 6 and the recesses 77 are not limited to those described above, the size of the petri dish, the type of microorganisms or living cells to be cultured, etc. Is set as appropriate.
In the above embodiment, the slits 9 and 11 are formed in the instrument bodies 5 and 73, and the hoses 13 and 15 are held in the slits 9 and 11, but instead of forming the slits 9 and 11, holes are formed. The hoses 13 and 15 may be passed through the holes so that one end communicates with the hole 6 of the instrument body 5 or the recess 77 of the instrument body 73.

It is a perspective view of the petri dish which stores the culture solution storage tool concerning Embodiment 1 of this invention, and this culture solution storage tool. It is AA sectional drawing of FIG. It is BB sectional drawing of FIG. It is a perspective view of the state which accommodated the culture solution storage tool of FIG. 1 in the petri dish. It is a perspective view of the state which accommodated the petri dish which accommodated the culture solution storage tool of FIG. 4 in the incubator for microscope observation. It is GG sectional drawing of FIG. It is a perspective view of the petri dish which accommodates the culture solution storage tool which concerns on Embodiment 2 of this invention, and this culture solution storage tool. It is AA sectional drawing of FIG. It is sectional drawing of the culture solution storage tool which concerns on Embodiment 3 of this invention. It is sectional drawing of the state which accommodated the culture solution storage tool which concerns on Embodiment 3 of this invention in the petri dish.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1, 71, 81 Culture solution storage tool 2 Petri dish 3 Petri dish lid 4 Convex part 5, 73 Instrument main body 6 Round hole 7 Concave part 9, 11 Holding slit 13, 15 Hose 17 Connection pipe 19 Petri dish main body 20 Upper surface part of lid 22 Lid No. 25, 27 Cover housing 29, 31 Opening 33 Microscope observation incubator 35 Heating plate 37 Water tank unit 39 Lid 41 Housing 43 Heater 45 Round hole 47 Water tank unit 49 Water tank 51 Water supply pipe 53 Water supply hose 55 Gas supply pipe 57 Gas hose 58 Holding plate 59 Housing 61 Transparent glass heater 63 Fixed block 65 Petri dish body bottom portion 66 Recess 67 for culture solution storage 69 Hose 75 Bottom 77 Recess S Microscope stage Sa Stage hole C Culture Liquid D Living cell E Condenser The thickness P2 dish inside diameter T2 dish depth of the body of the main body of the instrument body having a diameter T culture fluid storage appliances of the equipment main body of the objective lens P culture solution storage appliance

Claims (12)

  A culture solution storage device comprising: a device main body installed and accommodated on a bottom surface portion of a petri dish main body; and a culture solution storage hole or recess formed in the device main body.   The culture solution storage device according to claim 1, wherein at least two hoses having one end communicating with the hole or the recess are provided.   The culture solution storage device according to claim 1 or 2, wherein the device body is made of an elastically deformable material.   4. The culture solution storage device according to claim 3, wherein an adsorption recess is formed on a bottom surface of the device body.   The culture solution storage device according to any one of claims 1 to 4, wherein the device main body is made of a material capable of seeing through the inside of the hole or the recess from the outside.   The culture medium storage device according to any one of claims 1 to 5, wherein the device main body is formed in a disc shape having a diameter slightly smaller than an inner diameter of a circular petri dish main body in which the device main body is accommodated. An apparatus for storing a culture solution.   The culture solution storage device according to any one of claims 1 to 6, wherein the hole or the recess is provided in a central portion of the device body.   The culture solution storage device according to any one of claims 2 to 7, wherein one end portion communicating with at least one hole or recess of the hose is higher than one end portion communicating with the hole or recess of another hose. A culture medium storage device characterized by being arranged.   A petri dish cover that covers an upper surface opening of a petri dish main body in which the instrument main body of the culture solution storage instrument according to any one of claims 2 to 8 is accommodated, and a drawer port is provided for drawing the hose out of the petri dish. Petri dish lid characterized by   The petri dish lid according to claim 9, wherein the outlet is formed by a notch continuous from an upper surface portion to a peripheral surface portion of the petri dish lid.   The petri dish lid according to claim 9 or 10, further comprising a cover portion that is located above the drawer opening and covers an upper surface of the drawer opening. A petri dish comprising the petri dish lid according to any one of claims 9 to 11 and a petri dish body whose upper surface opening is covered by the petri dish lid.

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