JP3082912B2 - Livestock egg maturation, fertilization, developmental incubator - Google Patents
Livestock egg maturation, fertilization, developmental incubatorInfo
- Publication number
- JP3082912B2 JP3082912B2 JP09318519A JP31851997A JP3082912B2 JP 3082912 B2 JP3082912 B2 JP 3082912B2 JP 09318519 A JP09318519 A JP 09318519A JP 31851997 A JP31851997 A JP 31851997A JP 3082912 B2 JP3082912 B2 JP 3082912B2
- Authority
- JP
- Japan
- Prior art keywords
- carbon dioxide
- container body
- incubator
- livestock
- valve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 244000144972 livestock Species 0.000 title claims description 9
- 230000004720 fertilization Effects 0.000 title claims description 5
- 230000035800 maturation Effects 0.000 title claims description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 74
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 37
- 239000001569 carbon dioxide Substances 0.000 claims description 37
- 235000013601 eggs Nutrition 0.000 claims description 10
- 210000001672 ovary Anatomy 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 210000004681 ovum Anatomy 0.000 claims description 6
- 230000018109 developmental process Effects 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 2
- 108010000912 Egg Proteins Proteins 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 125000005587 carbonate group Chemical group 0.000 claims 1
- 239000003761 preservation solution Substances 0.000 claims 1
- 239000007789 gas Substances 0.000 description 18
- 239000000126 substance Substances 0.000 description 10
- 239000003755 preservative agent Substances 0.000 description 5
- 230000002335 preservative effect Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 241000283690 Bos taurus Species 0.000 description 3
- 210000002257 embryonic structure Anatomy 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 229920003002 synthetic resin Polymers 0.000 description 3
- 239000000057 synthetic resin Substances 0.000 description 3
- 230000013020 embryo development Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 241000283086 Equidae Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000007792 gaseous phase Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 150000002926 oxygen Chemical class 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【０００１】[0001]
【発明の属する技術分野】本発明は牛、馬、豚等の家畜
類の卵子を、成熟、受精、発生させ、移植可能な胚を作
出する輸送可能な成熟培養器に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a transportable mature incubator which matures, fertilizes, and develops eggs of livestock such as cattle, horses, pigs, etc., and produces transplantable embryos.
【０００２】[0002]
【従来の技術】従来、牛の体外受精胚を作出するのに使
用する卵子は屠場で屠殺した牛より卵巣を取り出し、そ
の卵巣を保存液に入れて処理室に持ち帰り、そこで卵巣
から未成熟卵子を採取し、温度３８．５℃，５％炭酸ガ
ス気相条件下で成熟，体外受精，体外発生しているのが
実情である。2. Description of the Related Art Conventionally, ova used for producing in vitro fertilized embryos of cattle are obtained by removing ovaries from cattle slaughtered in a slaughterhouse, placing the ovaries in a preservative solution, and bringing them back to a processing room where the immature ova are extracted from the ovaries. It is a fact that maturation, in vitro fertilization and extracorporeal development occur at a temperature of 38.5 ° C. and 5% CO 2 gas phase.
【０００３】そして、作出した体外受精卵は保存液に収
容した状態で輸送用容器やストロー等に封入して輸送し
ている。この時使用される培養輸送器としては、断熱構
造の容器内にバッテリー、ヒーターを内蔵し、且つ家畜
胚は器具や設備が完備した処理室で調整してストロー管
に収めて前記培養輸送器で輸送するものである。[0003] The produced in vitro fertilized eggs are transported in a state of being stored in a preservative solution, sealed in a transport container, a straw or the like. As a culture transporter used at this time, a battery and a heater are built in a container having an insulated structure, and livestock embryos are adjusted in a processing room equipped with instruments and equipment and stored in a straw tube. To transport.
【０００４】[0004]
【発明が解決しようとする課題】しかしながら、家畜類
の卵子は環境変化によって大きな影響を受けやすく、上
記した従来の方法では屠場から処理室までの輸送距離が
長ければ長いほど卵巣の中の未成熟卵子の生存能力や発
育能力が低下し、最悪の場合は輸送途中や輸送後に卵子
が死んだり、死なないまでも生存能力、発育能力が大幅
に低下して目的の用途に活用できないといった問題を有
する。However, eggs of livestock are easily affected by environmental changes, and in the above-mentioned conventional method, the longer the transport distance from the slaughterhouse to the processing room, the more immature the ovaries will be. Eggs will lose their ability to survive and grow, and in the worst case, they will die during and after transportation, or will have a problem that they will not be able to be used for their intended purpose, even if they do not die. .
【０００５】本発明は上述した従来の技術が有する問題
点に鑑みてなされたもので、その課題とするところは、
屠場で取り出した家畜類の卵巣から採取した卵子を培養
しながら活力に富む状態で成熟，受精並びに発生培養さ
せ目的地に運ぶことが出来る簡易培養器を提供すること
にある。又、他の目的は非常に簡単な構造で安価に提供
することが出来る簡易培養器にある。[0005] The present invention has been made in view of the above-mentioned problems of the prior art.
It is an object of the present invention to provide a simple incubator capable of carrying ovum collected from the ovaries of livestock taken out at a slaughterhouse while being cultured, fertilizing, developing and culturing in a vibrant state, and transporting the ovary to a destination. Another object is to provide a simple incubator which can be provided at a low cost with a very simple structure.
【０００６】[0006]
【課題を解決するための手段】上記の課題を解決するた
めに本発明が講じた技術的手段は、家畜の卵巣より取り
出した未成熟卵子（以下、卵子という）を保存液と共に
収容するシャーレを出し入れ可能に収納する密閉蓋体を
有した携帯可能な容器本体に、該容器本体内を陰圧にす
る吸引器を接続するための逆止弁を備えたバルブを取り
付け、且つ容器本体内は炭酸ガスパウダーを注入して所
定の炭酸ガス濃度とすることを特徴とする。Means for Solving the Problems The technical measures taken by the present invention to solve the above-mentioned problems include a petri dish containing an immature egg (hereinafter referred to as an egg) taken out of the ovary of a domestic animal together with a preservative solution. A valve provided with a check valve for connecting a suction device that makes the inside of the container body a negative pressure is attached to a portable container body having a closed lid that can be taken in and out, and the inside of the container body is carbonated. by injecting Gasupauda chromatography, characterized in that the predetermined carbon dioxide concentration.
【０００７】上記の容器本体は、容体と密閉可能なシー
ル機構を備えた蓋体とからなり、それら容体と蓋体は合
成樹脂製、金属製の何れでもよい。尚、合成樹脂製とし
た場合は内部を透視できる透明又は半透明とすること
で、炭酸ガスを発生させるための薬品（例えば、堀井薬
品工業株式会社製の炭酸ガスパウダー）を該容器本体内
のシャーレに投下収納する操作を確実に行うことが出来
る。The above-mentioned container body comprises a container and a lid provided with a sealable seal mechanism. The container and the lid may be made of synthetic resin or metal. When made of synthetic resin, the inside of the container body is made transparent or translucent so that a chemical for generating carbon dioxide (for example, carbon dioxide powder manufactured by Horii Pharmaceutical Co., Ltd.) is formed in the container body. The operation of dropping and storing in a petri dish can be reliably performed.
【０００８】又、上記炭酸ガス発生用の薬品の投入は密
閉蓋体を外して容体内のシャーレに入れてもよいが、発
生した炭酸ガスの漏れを最小限に止めるためには容器本
体の蓋体に、炭酸ガスパウダーを容器本体内のシャーレ
内に投入する通孔を開設し、その通孔に栓体を着脱自在
に取り付けるようにしてもよい。更に、上記容器本体内
を所定の炭酸ガス濃度（５％濃度）に調整する手段とし
ては、薬品（炭酸ガスパウダー）をシャーレ内の水と反
応させて発生させる形態に限らず、容器本体内に直接炭
酸ガスボンベ等から注入してもよく、その注入用のバル
ブを取り付けてもよい。The above-mentioned chemical for generating carbon dioxide may be introduced into a petri dish inside the container by removing the closed lid. However, in order to minimize the leakage of the generated carbon dioxide, the lid of the container body is required. The body may be provided with a through-hole through which carbon dioxide powder is charged into a petri dish in the container body, and a plug may be removably attached to the through-hole. Further, the means for adjusting the inside of the container body to a predetermined carbon dioxide gas concentration (5% concentration) is not limited to a mode in which a chemical (carbon dioxide gas powder) is reacted with water in a petri dish to generate the same. It may be directly injected from a carbon dioxide gas cylinder or the like, or a valve for the injection may be attached.
【０００９】[0009]
【発明の実施の形態】以下、本発明の実施の形態を図面
に基づいて説明する。図１は本発明に係る簡易培養器を
示す斜視図で、図中、Ａは合成樹脂材で成形された小形
の携帯可能な容器本体で、卵子又は胚を保存液と共に収
容したシャーレＢを収容し得るある程度の深さを有した
容体１と、その容体１の上面開口部を閉鎖する密閉可能
なシール機構を備えた蓋体２とで構成され、前記容体１
と蓋体２には該蓋体２を密閉状態に掛け止め保持する掛
け止め手段３が取り付けられている。又、容器本体Ａを
構成する蓋体２は内部を透視できるように透明又は半透
明とし、且つその蓋体２の適宜位置には、炭酸ガスを発
生させるための薬品（例えば、堀井薬品工業株式会社製
の炭酸ガスパウダー）を容器本体内の水を入れたシャー
レＢ’に投入する通孔４が開設され、その通孔４は着脱
自在な栓体５で開閉自在に構成されている。Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a perspective view showing a simple incubator according to the present invention. In the figure, A is a small portable container main body formed of a synthetic resin material, and accommodates a petri dish B containing an egg or an embryo together with a preservative solution. A container 1 having a certain depth and a lid 2 provided with a sealable seal mechanism for closing an upper opening of the container 1.
The lid 2 is provided with latch means 3 for latching and holding the lid 2 in a sealed state. The lid 2 constituting the container body A is transparent or translucent so that the inside can be seen through, and a chemical for generating carbon dioxide gas (for example, Horii Pharmaceutical Co., Ltd.) is provided at an appropriate position on the lid 2. A through hole 4 for introducing a carbon dioxide gas powder (made by a company) into a petri dish B ′ containing water in the container body is opened, and the through hole 4 is configured to be openable and closable by a removable plug 5.
【００１０】そして、上記容器本体Ａにおける容体１の
周壁には、密閉された容器本体Ａ内を所定の陰圧にする
吸引器Ｃに接続されたホース先端の口金８を接続するた
めのバルブ６と、容器本体Ａ内を所定の炭酸ガス濃度の
気相とするための炭酸ガスを直接該容器内に注入するた
めの炭酸ガスボンベ（図示省略）に接続されたホース口
金９を接続するためのバルブ７が取り付けられている。A valve 6 for connecting a base 8 at the end of a hose connected to a suction device C for making the inside of the sealed container body A a predetermined negative pressure is provided on the peripheral wall of the container 1 in the container body A. And a valve for connecting a hose base 9 connected to a carbon dioxide gas cylinder (not shown) for directly injecting carbon dioxide into the container so as to inject a carbon dioxide gas into the container into a gaseous phase having a predetermined carbon dioxide concentration. 7 is attached.
【００１１】上記のバルブ６，７はそれぞれ逆止弁を備
え、それら逆止弁は吸引器のホース、或いは炭酸ガスボ
ンベのホースが接続されることで開き、その接続したホ
ースを外すことで逆止弁が閉じる今日周知のものであ
る。尚、前記バルブ６，７は手動切り替えのコックを備
えたバルブでもよく、要は容器本体内を所定の気相（圧
力、炭酸ガス濃度）に調整保持できればよい。Each of the valves 6 and 7 has a check valve. The check valves are opened when a hose of a suction device or a hose of a carbon dioxide gas cylinder is connected, and the check is performed by removing the connected hose. The valve closes well known today. Note that the valves 6 and 7 may be valves provided with cocks for manual switching. In short, it is only necessary that the inside of the container body can be adjusted and maintained at a predetermined gas phase (pressure and carbon dioxide concentration).
【００１２】次に上記した培養器の使用について説明す
る。図２乃至図４は容器本体Ａ内を所定の炭酸ガス濃度
にする手段として薬品を使用する形態を示し、先ず図２
に示すように、容器本体Ａ内に家畜の卵巣より取り出し
た卵子を保存液と共に入れたシャーレＢと、水を入れた
シャーレＢ’を収容し、且つ水を入れたシャーレＢ’は
蓋体２に開設した通孔４の下方に位置させ、通孔４から
シャーレＢ’内に炭酸ガスを発生する薬品Ｄ（例えば、
堀井薬品工業株式会社製の炭酸ガスパウダー）を投入す
る。Next, the use of the above-described incubator will be described. 2 to 4 show an embodiment in which a chemical is used as a means for adjusting the inside of the container body A to a predetermined carbon dioxide concentration.
As shown in the figure, a Petri dish B containing the eggs taken out of the ovaries of the livestock together with the preservative solution and a Petri dish B 'containing water are accommodated in the container body A, and the Petri dish B' containing water is covered with the lid 2. Is located below the through-hole 4 opened in the container D, and a chemical D that generates carbon dioxide gas from the through-hole 4 into the petri dish B ′ (for example,
Carbon dioxide powder manufactured by Horii Pharmaceutical Co., Ltd.).
【００１３】シャーレＢ’への薬品Ｄの投入後、通孔４
を栓体５で閉じ、容器本体Ａ内に炭酸ガスを発生させ
る。この炭酸ガスの発生はシャーレＢ’内に収納された
水と薬品Ｄが反応して炭酸ガスを発生する。薬品Ｄの投
入量は、例えばシャーレＢ’に５mlの水を入れ、炭酸ガ
スパウダーを０．５〜１．０ｇ投入する。そして、図３
に示すように容器本体Ａ内を所定の陰圧にするためにバ
ルブ６にホース口金８を介して吸引器Ｃを接続し、該吸
引器Ｃを操作して容器本体Ａ内を２０ｍｌ程度にする。
容器本体Ａ内を吸引器で陰圧にすることで該容器本体Ａ
内の酸素量は２１％から１５％まで下がり、この酸素量
と温度（３８．５℃）及び陰圧による緊張度が胚の発生
率を高める。そして、上記の条件下からシャーレＢを取
り出す時は、容器本体内に空気を注入して陰圧を解除
し、蓋体２を取り外し可能な状態とする。容器本体内を
常圧にする方法としては、バルブ７にホースを接続する
などして該バルブの逆止弁を開いて大気と連通させる。After the introduction of the chemical D into the petri dish B ', the through holes 4
Is closed with the stopper 5 to generate carbon dioxide gas in the container body A. The carbon dioxide is generated by reacting the water contained in the petri dish B 'with the chemical D to generate carbon dioxide. The dosage of the chemical D is, for example, 5 ml of water in a Petri dish B 'and 0.5 to 1.0 g of carbon dioxide powder. And FIG.
As shown in (1), a suction device C is connected to the valve 6 via a hose mouthpiece 8 in order to make the inside of the container body A a predetermined negative pressure, and the suction device C is operated to make the inside of the container body A about 20 ml. .
By making the inside of the container body A negative pressure with a suction device, the container body A
The oxygen content in the cell drops from 21% to 15%, and this oxygen content and the degree of tension caused by temperature (38.5 ° C.) and negative pressure increase the incidence of embryo development. Then, when the petri dish B is taken out under the above conditions, the negative pressure is released by injecting air into the container body, and the lid 2 is set in a removable state. As a method for adjusting the inside of the container body to normal pressure, a check valve of the valve is opened by connecting a hose to the valve 7 or the like, and the container is communicated with the atmosphere.
【００１４】図５及び図６は容器本体Ａ内を所定の炭酸
ガス濃度にする手段として炭酸ガスを直接容器内に注入
する形態を示し、この場合は容器本体Ａに設けたバルブ
７に炭酸ガスのボンベを接続して注入する。そして、炭
酸ガスを注入した後前記した実施の形態と同様バルブ６
に吸引器を接続して容器本体内を所定の陰圧にする。FIGS. 5 and 6 show an embodiment in which carbon dioxide gas is directly injected into the container as a means for bringing the inside of the container body A to a predetermined carbon dioxide concentration. In this case, the valve 7 provided in the container body A is supplied with carbon dioxide gas. Connect the cylinder and inject. Then, after injecting the carbon dioxide gas, the valve 6 is similar to the above-described embodiment.
To a predetermined negative pressure inside the container body.
【００１５】[0015]
【発明の効果】本発明の家畜卵子の成熟・受精・発生培
養器は請求項１に記載の構成により、簡単な構成で胚発
生の好条件を保ちながら輸送が可能な簡易培養器を提供
できる。また、請求項２に記載の構成により、薬品（炭
酸ガスパウダー）を使用しての炭酸ガスの発生を蓋体を
したままで迅速且つ確実に行うことが出来る。更に、請
求項３に記載の構成により、炭酸ガスを容器本体内に直
接注入することが可能となり、幅広い使用が可能とな
る。According to the present invention, the incubator for maturation, fertilization and development of domestic eggs can provide a simple incubator which can be transported with a simple configuration while maintaining favorable conditions for embryo development. . Further, according to the configuration of the second aspect, the generation of carbon dioxide using a chemical (carbon dioxide powder) can be performed quickly and reliably with the lid kept. Furthermore, according to the configuration of the third aspect, the carbon dioxide gas can be directly injected into the container main body, so that it can be used widely.
【図１】本発明に係る成熟培養器の一例を示す斜視図で
ある。FIG. 1 is a perspective view showing an example of a mature incubator according to the present invention.
【図２】容器本体内のシャーレに炭酸ガスパウダーを投
入する状態を示す断面図である。FIG. 2 is a cross-sectional view showing a state in which carbon dioxide powder is charged into a petri dish in a container body.
【図３】容器本体のバルブに吸引器を接続して容器内を
所定の陰圧にする状態を示す断面図である。FIG. 3 is a cross-sectional view showing a state in which a suction device is connected to a valve of the container main body to make a predetermined negative pressure inside the container.
【図４】容器本体内を常圧に戻す状態を示す断面図であ
る。FIG. 4 is a sectional view showing a state in which the inside of the container body is returned to normal pressure.
【図５】容器本体内に直接炭酸ガスを注入を注入し、内
部を所定の陰圧にする状態を示す一部切欠平面図であ
る。FIG. 5 is a partially cutaway plan view showing a state in which carbon dioxide gas is directly injected into the container main body and a predetermined negative pressure is applied to the inside.
【図６】同縦断面図である。FIG. 6 is a longitudinal sectional view of the same.
Ａ…容器本体 Ｂ，Ｂ’…シャーレ １…容体 ２…蓋体 ４…通孔 ５…栓体 ６…吸引器接続用バルブ ７…炭酸ガスボン
ベ接続用バルブA: Container body B, B ': Petri dish 1 ... Container 2 ... Lid 4 ... Through-hole 5 ... Plug body 6 ... Valve for connecting suction device 7 ... Valve for connecting carbon dioxide gas cylinder
Claims (3)
保存液と共に収容するシャーレを出し入れ可能に収納す
る密閉蓋体を有した携帯可能な容器本体に、該容器本体
内を陰圧にする吸引器を接続するための逆止弁を備えた
バルブを取り付け、且つ容器本体内は炭酸ガスパウダー
を注入して所定の炭酸ガス濃度とすることを特徴とする
家畜卵子の成熟、受精、発生培養器。1. A suction device for applying a negative pressure to a portable container body having a closed lid for accommodating a petri dish for accommodating an immature ovum taken out from an ovary of a domestic animal together with a preservation solution so as to be able to be taken in and out. attaching a valve with a check valve for connecting the vessel and the vessel body is carbonate Gasupauda over
Culturing, fertilizing, and developing incubating livestock ova, wherein a predetermined concentration of carbon dioxide gas is injected into the cultivator.
ーを容器本体内の水を入れたシャーレ内に投入する通孔
を開設し、その通孔に栓体を着脱自在に取り付けた請求
項１記載の家畜卵子の成熟、受精、発生培養器2. A through hole for introducing carbon dioxide powder into a petri dish filled with water in the container body is formed in the lid of the container body, and a stopper is detachably attached to the through hole. Maturation, fertilization, developmental incubator of livestock ova according to 1.
ブを取り付けた請求項１又は請求項２記載の家畜卵子の
成熟、受精、発生培養器。3. The incubator for maturation, fertilization and development of livestock eggs according to claim 1 or 2, wherein a valve for injecting carbon dioxide gas is attached to said container body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP09318519A JP3082912B2 (en) | 1997-11-19 | 1997-11-19 | Livestock egg maturation, fertilization, developmental incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP09318519A JP3082912B2 (en) | 1997-11-19 | 1997-11-19 | Livestock egg maturation, fertilization, developmental incubator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH11151083A JPH11151083A (en) | 1999-06-08 |
| JP3082912B2 true JP3082912B2 (en) | 2000-09-04 |
Family
ID=18100024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP09318519A Expired - Fee Related JP3082912B2 (en) | 1997-11-19 | 1997-11-19 | Livestock egg maturation, fertilization, developmental incubator |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3082912B2 (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4855778B2 (en) * | 2005-01-31 | 2012-01-18 | 知 藤澤 | Egg observation device |
| JP4626768B2 (en) * | 2006-04-28 | 2011-02-09 | 三菱瓦斯化学株式会社 | Method for microscopic observation of culture state |
| FR2905675B1 (en) | 2006-09-13 | 2008-11-21 | Biopredic Internat | METHOD FOR CONDITIONING CELLS IN CULTIVATION IN A DEPRESSIONARY ENVIRONMENT, AND CORRESPONDING DEVICE |
| JP2009125062A (en) * | 2007-11-28 | 2009-06-11 | Hiyoshi:Kk | Simple portable incubator |
| JP5476557B2 (en) * | 2011-08-19 | 2014-04-23 | 株式会社日立製作所 | Packaging container and sample observation method using the same |
| JP6061890B2 (en) * | 2014-03-28 | 2017-01-18 | 株式会社ヤクルト本社 | Tank breakage prevention mechanism |
| CN109022264B (en) * | 2018-04-16 | 2024-09-03 | 倪少石 | Auxiliary device capable of improving survival rate of ovum |
| CN112625881A (en) * | 2021-01-12 | 2021-04-09 | 曹增育 | Negative pressure culture apparatus convenient to collagen draws |
-
1997
- 1997-11-19 JP JP09318519A patent/JP3082912B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH11151083A (en) | 1999-06-08 |
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