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JP2022511931A - 細胞および生体分子上へのオリゴヌクレオチドの直接合成 - Google Patents

細胞および生体分子上へのオリゴヌクレオチドの直接合成 Download PDF

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JP2022511931A
JP2022511931A JP2021533202A JP2021533202A JP2022511931A JP 2022511931 A JP2022511931 A JP 2022511931A JP 2021533202 A JP2021533202 A JP 2021533202A JP 2021533202 A JP2021533202 A JP 2021533202A JP 2022511931 A JP2022511931 A JP 2022511931A
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cell
oligonucleotide
extended
cells
initiator
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シャビエル ゴドロン，
アドリアン ホルガン，
シルヴァン ガリエル，
ジェフリー ジェドロー，
ロベール ニコル，
トマス イベール，
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ディーエヌエー スクリプト
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JP2021533202A 2018-12-13 2019-12-10 細胞および生体分子上へのオリゴヌクレオチドの直接合成 Pending JP2022511931A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP18306687.7 2018-12-13
EP18306687 2018-12-13
EP19305219.8 2019-02-25
EP19305219 2019-02-25
PCT/EP2019/084347 WO2020120442A2 (fr) 2018-12-13 2019-12-10 Synthèse d'oligonucléotides directe sur des cellules et des biomolécules

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JP2022511931A true JP2022511931A (ja) 2022-02-01

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US (3) US11268091B2 (fr)
EP (2) EP3894593B1 (fr)
JP (1) JP2022511931A (fr)
KR (1) KR20210104779A (fr)
CN (1) CN113423841A (fr)
AU (1) AU2019398307A1 (fr)
CA (1) CA3122494A1 (fr)
IL (1) IL283835A (fr)
SG (1) SG11202105441WA (fr)
WO (1) WO2020120442A2 (fr)

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US11359221B2 (en) * 2019-02-12 2022-06-14 Dna Script Sas Efficient product cleavage in template-free enzymatic synthesis of polynucleotides
US11365441B2 (en) 2019-05-22 2022-06-21 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
WO2021018921A1 (fr) * 2019-08-01 2021-02-04 Dna Script Augmentation des rendements de séquence longue dans la synthèse enzymatique sans matrice de polynucléotides
EP4139446A1 (fr) * 2020-04-20 2023-03-01 DNA Script Variants de désoxynucléotidyl transférase et utilisations correspondantes
US20230313255A1 (en) 2020-07-15 2023-10-05 Dna Script Massively Parallel Enzymatic Synthesis of Polynucleotides
EP4217476A1 (fr) 2020-09-22 2023-08-02 DNA Script Variants stabilisés de désoxynucléotidyl transférase terminale tronqués à l'extrémité n-terminale et utilisations associées
US20220145345A1 (en) * 2020-11-11 2022-05-12 Microsoft Technology Licensing, Llc Spatial control of polynucleotide synthesis by strand capping
EP4274907A4 (fr) 2021-01-08 2025-01-15 Cellanome, Inc. Dispositifs et procédés pour analyser des échantillons biologiques
IL307885A (en) 2021-04-23 2023-12-01 Ganna Bio Inc Nucleic acids with different glycans, preparation methods and therapeutic uses
EP4352246A4 (fr) * 2021-06-11 2025-06-25 Mission Bio, Inc. Procédé, système et appareil d'analyse d'un analyte d'une cellule unique
WO2023275334A1 (fr) * 2021-07-01 2023-01-05 Miltenyi Biotec B.V. & Co. KG Composition d'adn unitaire pour le codage et le séquençage spatiaux
CN116555216A (zh) * 2022-01-28 2023-08-08 中国科学院天津工业生物技术研究所 可控合成单链dna的末端转移酶变体及应用
CN119546760A (zh) * 2022-07-13 2025-02-28 Dna斯克瑞普特公司 纯化包含多核苷酸的溶液的方法
WO2024141628A1 (fr) 2022-12-31 2024-07-04 Dna Script Encres à viscosité variable pour l'administration par jet d'encre de réactifs enzymatiques
WO2024146937A1 (fr) 2023-01-06 2024-07-11 Dna Script Procédés d'obtention d'acides nucléiques correctement assemblés
EP4530360A1 (fr) * 2023-09-29 2025-04-02 Miltenyi Biotec B.V. & Co. KG Procédé de codage à barres spatial
CN119662753A (zh) * 2025-02-19 2025-03-21 天津中合基因科技有限公司 抗体偶联寡核苷酸酶促dna原位合成方法

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WO2016040594A1 (fr) * 2014-09-10 2016-03-17 The Regents Of The University Of California Reconstruction de cellules ancestrales par enregistrement enzymatique
WO2017009663A1 (fr) * 2015-07-15 2017-01-19 Nuclera Nucleics Ltd Procédé de déprotection de l'éther azidométhylique

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