JP2018193336A - Skin external preparation - Google Patents

Abstract

To provide a skin external preparation and a hair composition containing components that have a skin whitening effect, a skin moisture effect, and a hair growth effect, and cause no skin irritation and have excellent biological safety.SOLUTION: The present invention provides a skin external composition or a hair composition containing fermentation products as an active ingredient, the fermentation products obtained by fermenting plants of Liliaceae, Hemerocallis or extract thereof, or water-soluble solvent extract of the plants, with lactic acid bacteria, yeast, koji mold or Bacillus natto.SELECTED DRAWING: None

Description

  The present invention is a novel active ingredient derived from a natural product and having excellent physiological activity and biosafety, a skin (including scalp) external preparation, and a food or drink for beauty or health promotion comprising the same.

  In recent years, research has been conducted on cell aging and external factors (for example, ultraviolet rays, chemical substances such as air pollutants and environmental hormones, allergens such as pollen, environmental stress, etc.), and various cells have been studied. The mechanism of aging, cell damage and its prevention and recovery has been elucidated.

  For example, regarding skin cells, internal factors such as inactivation of cell growth / differentiation associated with aging, decrease in hormone secretion, quantitative decrease in extracellular matrix components (collagen, laminin, etc.) constituting the skin, and the sun Aging phenomenon (wrinkles, tarmi, etc.) intricately intertwined with external factors such as active oxygen induced by light (ultraviolet rays), chemical substances such as air pollutants and environmental hormones, allergens such as pollen, and environmental stress Rough skin and color changes occur.

  Furthermore, ultraviolet rays, chemical substances, and allergens, which are external factors, damage cells and tissues in the living body, alter biological components (proteins, etc.), or generate active oxygen. As a result, antigens and cytokines that cause inflammation are generated in the living body, and lipid peroxides that cause acne and sunburn are generated in the skin. Furthermore, the above external factors induce abnormal deposition of intracellular melanin pigments, causing spots, freckles, liver spots and the like on the skin.

  Further, as described above, due to the internal factor and the external factor, the turnover of the epidermis becomes irregular, and the barrier function is deteriorated and the texture is disturbed.

  Various active ingredients have been proposed for the purpose of preventing and improving the unhealthy and aging of cells as described above, and cosmetics, foods and drinks and pharmaceuticals containing these active ingredients have been put on the market. For example, antioxidants such as vitamin C, vitamin E, and catalase; anti-inflammatory agents such as glycyrrhizic acid or salts thereof, allantoin, tranexamic acid; various ultraviolet absorbers; α-hydroxycarboxylic acid, placenta extract, γ-amino- Cell activation components such as β-hydroxybutyric acid; extracellular matrix components such as collagen, elastin, hyaluronic acid or salts thereof; humectants such as urea; and protein glycation inhibitors such as aminoguanidine. In addition, kojic acid, linoleic acid, and the like are known as substances that suppress the occurrence of pigmentation such as skin spots, buckwheat, and liver spots, and are widely used as active ingredients of whitening agents.

  As described above, conventionally, cell activators, anti-aging agents and whitening agents have been proposed based on the aging phenomenon and unhealthy mechanism of cells. There are problems in terms of effectiveness in application or taking. Accordingly, there is a need for an active ingredient that improves these conventional problems.

  As a result of diligent research to solve the above problems, the present inventors have found that plants or extracts belonging to the genus Hemerocallis, or fermented products thereof, have a remarkable effect of normalizing skin turnover. And newly found to have a hair-growth effect. Furthermore, it has also been found that these extracts or fermented products have an effect of promoting the synthesis of vitamin C transporter (SVCT1) involved in the incorporation of vitamin C into cells.

Conventionally, the invention of using an extract or fermented product of a plant belonging to the genus Hemerocallis in a cosmetic is known from Patent Documents 1 to 3, for example. However, a plant belonging to the genus Forget-me-not, or an extract thereof, a hydrolyzate of the extract, or a fermented product of the plant has a skin turnover normalizing effect, an SVCT1 synthesis promoting effect, and a hair growth effect. It was not known.
JP 2006-052165 JP 2006-143670 JP2008-050326

The present invention is an external composition for skin containing as an active ingredient a fermented product obtained by fermenting a plant belonging to the genus Hemerocallis or an extract thereof with lactic acid bacteria, koji molds or natto bacteria.
The present invention also provides a whitening composition comprising as an active ingredient a fermented product obtained by fermenting a plant belonging to the genus Hemerocallis or an extract thereof with yeast.
The present invention is also a moisturizing composition comprising as an active ingredient a fermented product obtained by fermenting a plant belonging to the genus Hemerocallis or an extract thereof with yeast.
The present invention is a composition for hair cosmetics comprising as an active ingredient a fermented product obtained by fermenting a plant belonging to the genus Hemerocallis or an extract thereof with lactic acid bacteria, yeast, koji mold or natto.
The present invention is an external composition for skin comprising as an active ingredient a water-soluble solvent extract of a plant belonging to the genus Hemerocallis.

  Since the extract or fermented product according to the present invention has an effect of promoting the synthesis of type XVII collagen, which is a basement membrane protein synthesized by skin epidermal cells, the composition containing any of them is composed of epidermal cells and Strengthens the adhesion of the basement membrane, which in turn contributes to normalizing the epidermis turnover. In addition, this effect keeps the state of the epidermis healthy, promotes rapid discharge of melanin synthesized in pigment cells, and contributes to rapid expression of whitening effect. It is also suggested that the effect of preventing hair loss and white hair can be achieved by inducing the expression of type XVII collagen in the scalp.

  Furthermore, since the fermented product according to the present invention can enhance the expression of SVCT-1, which is the intake port of vitamin C in pigment cells, it can be used in combination with vitamin C as a whitening active ingredient, to the pigment cells. It is possible to obtain a more effective whitening effect.

Hereinafter, the present invention will be described in detail.
Examples of the plant belonging to the genus Forget-me-not used in the present invention include, for example, Honkanllis (Hemerocallis fulva var. Fulva), Yakukan elephant (Hemerocallis fulva var. Kwanso), Hakukan elephant (Hemerocallis fulva var. , Hemerocallis fulva (Hemerocallis fulva), Hemerocallis citrina Baroni, Hemerocallis dumortieri var. Dumortieri, Hemerocallis lilioasp ), Ezokisge (Hemerocallis lilioasphodelus var. Yezoensis), Nikko Kusuge (Hemerocallis dumortieri var. Esculenta), Yuusuge (Hemerocallis citrina var. Vespertina), or Hemerocallis plicata.

  When a lilyaceae forget-me-not plant is used to obtain a fermented product by an extract or a microorganism (yeast, lactic acid bacteria, koji mold or natto), the fermentation site of the plant is not particularly limited, and the whole grass, leaves, flowers, Although appropriate parts such as stamens, pistils, stems, rhizomes, seeds (grains) can be used, the use of petals, flower parts including buds or whole plants containing flower parts is preferable.

  When fermenting a forget-me-not plant, it can be carried out, for example, as follows. First, as an assimilation source for fermentation, a forget-me-not plant itself (hereinafter sometimes referred to as a plant) may be used, or an extract obtained by extracting a plant with an appropriate medium may be used. Good. Moreover, when using an extract, it is also possible to perform fermentation with a plant body contained, without removing the plant body of an extract by solid-liquid separation. Here, forget-me-not plants may be raw or may be dried or semi-dried in advance. Further, the collected shape can be used as it is.

  When fermentation is performed using a plant body as an assimilation source, the plant body is first suspended in a solvent. As a solvent for suspending the plant body, water or water and lower alcohols (methanol, ethanol, isopropanol, etc.) or glycols (ethylene glycol, 1,3-propanediol, 1,3-butylene glycol, 1, 2-pentanediol, glycerin, etc.) can be used. In these solvents, saccharides such as glucose, fructose, sucrose may be added. Here, the mixing ratio of the plant body and the solvent is generally in the range of 1: 1 to 1: 100, preferably 1:10 to 1:50 in terms of the dry weight of the plant body.

  When using an extract of a plant body as a fermentation assimilation source, the preparation method of the extract is, as necessary, pre-washing a part to be extracted, such as a flower part (petals, buds, etc.), if necessary, and preferably further A method of contacting with an extraction solvent by an appropriate means such as a dipping method or a countercurrent extraction method after being chopped or crushed can be employed. A supercritical extraction method may also be used.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol and octyldodecanol; many solvents such as ethylene glycol, propylene glycol, 1,3-butylene glycol and glycerin Monohydric alcohols; esters such as ethyl acetate, butyl acetate, methyl propionate and glyceryl trioctanoate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether, isopropyl and ether; n-hexane, toluene, chloroform and the like A hydrocarbon solvent etc. are mentioned, These can be used individually or in mixture of 2 or more types.

  The mixing ratio in the case of using a mixed solvent is, for example, 1: 1 to 25: 1 by volume ratio (hereinafter the same) if the mixed solvent is water and ethyl alcohol, and 1: if the mixed solvent is water and glycerin. If it is 1 to 15: 1, or a mixed solvent of water and 1,3-propanediol or 1,3-butylene glycol, it is preferably in the range of 1: 1 to 15: 1. When a solvent other than water or a mixed solvent of water and a solvent other than water is used as the extraction solvent, the solvent other than water is once distilled off by an operation such as concentration before moving to the fermentation step, and then solid. It may be redissolved in water so as to be about 0.01 to 10% as a fraction, or diluted with water and used as a utilization source for fermentation.

  Examples of the microorganism used for the fermentation treatment of the suspension or extract solution prepared as described above include the following.

  Examples of lactic acid bacteria used for fermentation include Lactobacillus plantarum, L. brevis, Lactobacillus casei, Lactobacillus delbrueckii and other Lactobacillus genus Lactobacillus lactic acid bacteria. Lactic acid bacteria of the genus Carnobacterium such as Carnobacterium divergens, Carnobacterium piscicola; Leuconostoc mesenteroides, Leuconostoc lactis, Leuconostoc lactis Leuconostoc lactic acid bacteria such as Leuconostoc citreum; Streptococcus faecalis, Streptococcus piogenes (Streptococcus) lactic acid bacteria of the genus Streptococcus such as pyogenes; lactic acid bacteria of the genus Enterococcus such as Enterococcus caseliflavus and Enterococcus sulfreus; Lactococcus plantaact Lococcus plantaact Lactococcus lactic acid bacteria; Weissella confusa, Weissella kandleri, and other Weissella lactic acid bacteria; Atopobium minutum, Atopobium parvulus, etc. (Vagococcus fluvialis), Lactobacillus of the genus Vagococcus such as Vagococcus salmoninarum; Pediococca Damunosasu (Pediococcus damnosus), include lactic acid bacteria of the Pediococcus (Pediococcus) genus such as Pediococcus pentosaceus (Pediococcus pentosaceus).

  In the present invention, yeast refers to, for example, Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces cerevisias, Yeast of the genus, yeast of the genus Galactomyces, Torulaspora delbruekii, Torulaspora fermentati, Torulaspora rosei, Torulaspora rosey, etc. Zygosaccharomyces soya, Zygosaccharomyces sake, Zygosaccharomyces miso, Digosaccharomyces miso (Zygosaccharomyces lactis) and other yeasts of the genus Gigosaccharomyces, Candida versatilis, Candida etchellsii, Candida kefyr, Candida sake, and Candida scotty (C) Yeasts of the genus Aureobasidium such as Aureobasidium pullulans, Aureobasidium mansonii, Aureobasideium microstictum, etc. . The yeast according to the present invention may be any of sake yeast, wine yeast, beer yeast, plant flower (rose, lily, cherry tree, etc.) yeast, or sea-derived yeast.

  Examples of Aspergillus used for fermentation include Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, Aspergillus sojae, Aspergillus aspergillus, Aspergillus aspergillus sperm Examples include Aspergillus kawauchii, Aspergillus usami, Aspergillus niger and other black koji molds, Monascus anka and Monascus pilosus.

  Examples of Bacillus subtilis used for fermentation include Bacillus natto, Bacillus subtilis, Bacillus circulans, and the like.

  When the above-mentioned suspension or extract is fermented with microorganisms, it is necessary to sterilize the microorganisms before the fermentation step to remove the germs that hinder fermentation. As a method for sterilizing and removing the various bacteria, a method in which the fermentation material is washed with ethanol for sterilization in advance and then suspended in a sterile solvent such as sterile water may be used. The turbid liquid may be sterilized by heat sterilization or the like. As the heat sterilization treatment, the autoclave sterilization method in which the suspension is heated at 120 to 130 ° C. for 10 to 20 minutes, or holding at 80 to 90 ° C. for 60 to 120 minutes is repeated once a day for 2 to 3 days. The heat sterilization method such as the method is generally used.

The sterilized suspension is placed in a fermentation tank, in which microorganisms are inoculated and fermented. The appropriate amount of inoculated microorganism is 10 ⁷ to 10 ⁸ cells / mL. Even if the inoculation amount exceeds the above range, the fermentation progress time hardly changes. On the other hand, if the inoculation amount is less than the above range, it takes a long time to complete the fermentation.

  The fermentation temperature is generally in the range of 5 to 50 ° C., preferably 20 ° C. to 40 ° C., which is the optimum temperature for growth of each microorganism (for example, 30 ° C. to 40 ° C. for lactic acid bacteria, 25 ° C. to 30 ° C. for yeast). Range. The number of days of fermentation is generally in the range of 1 to 10 days, preferably 2 to 5 days, at the optimum temperature. If the fermentation days are shorter than the above general range, the fermentation is not sufficiently performed and the effectiveness of the fermented product tends to be reduced. On the other hand, if the fermentation days are longer than 10 days, no further increase in effectiveness is observed. Not only that, but also coloring and an increase in fermentation odor occur.

  When an extract is prepared from a forget-me-not plant, a hydrolysis treatment may be performed in order to improve its stability, effectiveness, and the like. In addition, when preparing a fermented product, the above suspension or extract solution is used before or simultaneously with inoculation of microorganisms so that the components of the target use site can be used more effectively as a source of microorganism utilization. Hydrolysis treatment may be performed on. Examples of the hydrolysis treatment include acid, alkali or enzyme treatment.

  When the hydrolysis treatment is performed using an enzyme, at least one enzyme selected from a proteolytic enzyme, a amylolytic enzyme, a pectin degrading enzyme, a fibrinolytic enzyme, and a lipolytic enzyme can be used as the enzyme. .

  Examples of proteolytic enzymes include actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, and peptidases such as glycylglycine peptidase, carboxypeptidase and aminopeptidase. Bromelain or the like can be used.

  As the starch degrading enzyme, for example, α-amylase, β-amylase, glucoamylase, β-galactosidase and the like can be used.

  Examples of pectin degrading enzymes that can be used include pectin depolymerase, pectin demethoxylase, pectin lyase, pectin esterase, and polygalacturonase.

  Examples of the fibrinolytic enzyme include cellulase, hemicellulase, agarase, mannase, chitinase, chitosanase, carrageenase, arginase, fucoidanase, inulase, xylanase, and ligninase.

  As the lipolytic enzyme, for example, lipase, phospholipase and the like can be used.

  The total amount of the enzyme used is preferably 0.01 to 10% by weight, more preferably 0.1 to 1.0% by weight, based on the solid content of the forget-me-not plant in the suspension. Or 0.001 to 5 weight% is preferable in total with respect to solid content of the extract of a forget-me-not plant, and 0.01 to 0.5 weight% is more preferable. As treatment conditions such as temperature and time, if the enzyme treatment is performed before fermentation, the treatment should be performed for about 1 to 24 hours near the optimum temperature of each enzyme. The same conditions as for the fermentation described above apply.

  After the above fermentation, if sterilization of microorganisms is used in combination with microbial sterilization, the sterilization of the fermentation broth is performed at 70 to 100 ° C. for about 10 to 120 minutes for both sterilization of microorganisms and inactivation of enzyme. After that, the liquid phase is separated as it is or generally and preferably by solid-liquid separation means such as filtration or centrifugation, and if necessary, the pH is adjusted to pH 6-8 which is the pH range of ordinary cosmetics. In addition, if necessary, it is adjusted to an appropriate concentration by dilution or concentration and used as a raw material for cosmetics. In some cases, the liquid phase after solid-liquid separation may be blended into a cosmetic after being powdered according to a conventional method such as spray drying or freeze drying.

  The extract or fermented product according to the present invention can be blended in a composition for external use (cosmetics, quasi-drugs, and external medicines), and a food or drink for cosmetics or health promotion. Examples of skin external preparations include emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, makeup press powders, blushers, white powders, facial cleansers, body shampoos, scalp, hair shampoos, hair conditioners. , Shampoos or tonics for hair growth, hair restoration, and cosmetics for cleaning such as soap, and bath preparations, and the like, but the present invention is not limited thereto. In addition, as food and drink for beauty or health promotion, drinks such as beauty drinks, nutrition drinks, sports drinks, near water, vitamin drinks, mineral drinks, alcoholic drinks; various soups (including powdered soups), dairy products , Jelly, candy, tablet confectionery, gum and other foods; tablets, liquids, granules or jelly-like health foods / beverages, etc., but the present invention is not limited to these and can be taken orally Can be blended into products

  The blending amount of the extract or fermented product according to the present invention is, for example, in the case of a basic cosmetic in the range of 0.002 to 1.0% by weight (solid content% by weight, hereinafter the same) as the solid content of the composition. In the case of makeup cosmetics, the range is 0.002 to 1.0% by weight, and in the case of cleansing cosmetics, the range is 0.002 to 10.0% by weight. Moreover, in the case of hair cosmetics, it is 0.0001 to 5.0 weight% as solid content of a composition. Moreover, the compounding quantity of the fermented material which concerns on this invention in food-drinks has the preferable range of 0.1-15 weight% as solid content of a composition.

  In addition to the essential fermented product, the composition containing the extract or fermented product according to the present invention is usually used. Ingredients used in external preparations for skin and foods and drinks, for example, oily ingredients, surfactants (synthetic and natural products), moisturizers, thickeners, antiseptic / bactericides, powder ingredients, antioxidants, chelating agents , PH adjusters, pigments, fragrances and the like can be appropriately blended as necessary. Moreover, as long as the effectiveness and characteristics are not impaired, there is no problem even if it is used in combination with other physiologically active ingredients.

  Here, as the oil component, for example, lotus oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice bran oil, rice germ oil, coconut oil, chamomile oil, palm oil, cacao oil, meadow foam oil, Oils derived from plants such as rosehip oil, lambender oil, sheer butter, tea tree oil, avocado oil, macadamia nut oil, plant-derived squalane; oils derived from animals such as mink oil and turtle oil; beeswax, carnauba wax, rice Waxes such as wax and lanolin; hydrocarbons such as liquid paraffin, petrolatum, paraffin wax and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid and eicosenoic acid; lauryl alcohol, cetanol, Higher alcohols such as stearyl alcohol; Isopro myristate Le, isopropyl palmitate, butyl oleate, 2-ethylhexyl glycerides, synthetic esters and synthetic triglycerides such as higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the like.

  As the surfactant, for example, polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, Nonionic surfactants such as polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates , Polyoxyethylene alkyl ether phosphate, α-sulfonated fatty acid alkyl ester salt, polyoxyethylene alkyl phenyl ether phosphate, etc. Surfactant; quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N, Cationic surfactants such as N-dialkylmorphonium salts and polyethylene polyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene Amphoteric surfactants such as ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

  Examples of emulsifiers or emulsifiers include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble Polysaccharides, soy-derived protein and polysaccharide complex, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their Derivatives, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, legumes, millet, etc.) and the like can also be blended.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, and saccharides such as trehalose, mucopolysaccharides (for example, Hyaluronic acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) ) Extracts, various amino acids and their derivatives.

  Examples of thickeners include, for example, brown algae such as alginic acid, agar, carrageenan, fucoidan, green algae, or red algae; silane root (white) extract; pectin, locust bean gum, aloe polysaccharide, alkaline-genes-producing polysaccharide, etc. Polysaccharides such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; hyaluron Examples include acid and derivatives thereof; polyglutamic acid and derivatives thereof.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, chloride Benzalkonium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), polyphosphoric acid, propanediol, 1,2-pentanediol, various essential oils, dry bark, radish fermented liquor, sugarcane, etc. There are plant-derived ethanol and 1,3-butylene glycol.

  Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, Cellulose powder, grains (rice, wheat, corn, millet, etc.) powder, beans (soybean, red beans, etc.) powder, and the like.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy- 4-methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. is there.

  Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, murasakixibub extract, silane root extract, peony extract, vitamin E and its derivatives (eg, vitamin E nicotinate, vitamin E linoleate) Etc.).

  Examples of the chelating agent include trisodium ethylenediaminehydroxyethyl triacetate, edetic acid or a salt thereof, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, tetrasodium hydroxyethane diphosphonate, and the like.

  Examples of the pH adjuster include citric acid or a salt thereof, lactic acid or a salt thereof, glycolic acid, succinic acid, hydrochloric acid, monoethanolamine, diethanolamine, triethanolamine, sodium hydroxide, potassium hydroxide and the like.

  Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof 4-methoxysalicylic acid potassium salt, magnolignan (5,5′-dipropyl-biphenyl-2,2′-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine mono) Phosphate, adenosine monophosphate), and these may be blended singly or in combination.

  Examples of the kojic acid derivative include kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid esters such as kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. As the ascorbic acid derivative, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid- Ascorbic acid ester salts such as 2-sulfate magnesium, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyl tocopheryl maleic acid, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbic acid Ascorbic acid sugar derivatives such as caprylyl 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups are hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L -L-ascorbic acid tetrafatty acid esters such as ascorbyl tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acyl thereof Derivatives, ascorbyl glycerol derivatives such as bisglyceryl ascorbic acid, aminopropyl phosphate L-ascorbate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl Examples of hydroquinone derivatives include phosphates such as arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), and tranexamic acid derivatives such as tranexamic acid esters (for example, tranexam). Acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), amides of tranexamic acid (for example, tranexamic acid methylamide) and the like. Examples of resorcinol derivatives include 4-n-butylresorcinol, Examples of 2,5-dihydroxybenzoic acid derivatives such as 4-isoamylresorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionylol. Examples of nicotinic acid derivatives include nicotinic acid amide and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. .

  Examples of the physiologically active component include placenta extract, Sakuhakuhi extract, Yukinoshita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, fermented white coconut, peony Extract or hydrolyzate thereof, lactic acid bacteria fermented rice, murasakixikib extract, lotus flower extract, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, pearl hydrolyzate, pearl seed fermented product, royal jelly fermentation Products, sake lees extract or ceramides contained therein, fermented sake lees, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract and the like. Also, coral grass extract, rice leaf extract or hydrolyzate thereof, eggplant (lotus, long eggplant, Kamo eggplant, rice eggplant etc.) extract or hydrolyzate thereof, apricot fruit extract, catamen giraffe, etc. Seaweed extract of sea urchin, extract of marine flowering plant such as sea cucumber, fermented soymilk, jellyfish water, rice extract or hydrolyzate thereof, rice fermented extract, germinated rice extract or hydrolyzate thereof, germinated rice fermented , Black bean extract or hydrolyzate thereof, damask rose flower extract, bamboo shoot peel extract, linoleic acid and its derivatives or processed products (eg liposomal linoleic acid), animal or fish-derived collagen and Derivatives thereof, elastin and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salts, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof, allantoin, diisopropyl Pyramine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, ginseng extract or fermented product thereof, red ginseng extract, beetroot extract, loofah extract, anaasa extract , Peach extract, Peach extract, Kiwi extract, Sunflower extract, Zizyphus joazeiro extract, Paudarco bark extract, Hibiscus flower extract or fermented product, Hagoromogusa extract, Cherimoya extract, Mango extract , Mangosteen extract, Funori extract, Oolong tea extract, Benifumi extract, Silane extract, Extract of yam peel or seed coat or hydrolysate, Safflower flower extract, Casablanca extract, Sweet potato extract or fermented product thereof , Guava leaf extract, dokudami extract, evening birch extract, aloe extract, fig flower extract , Apple extract, there is a white asparagus extract or the like.

  Next, the present invention will be described more specifically with reference to production examples, test examples, and examples, but the present invention is not limited thereto. In the following, all parts are by weight, and all% are by weight.

Production Example 1 Preparation of fermented product (1)
Purified water 1500 g, cellulase 1.5 g and pectinase 1.5 g were mixed with 150 g of Honkan elephant flower parts, extracted for 3 hours at 40 ° C. and subjected to enzymatic degradation, then heated at 80 ° C. for 1 hour, The enzyme was deactivated. Furthermore, the extract suspension obtained here was sterilized by heating. This extract suspension was inoculated with 10 ⁸ cells / mL of lactic acid bacteria (Lactobacillus plantarum), and left to stand at 37 ° C. for 3 days under a nitrogen stream. After culturing, the mixture was sterilized by heating, and the culture broth was filtered to perform deodorization and decolorization treatment to obtain 1123 g of a brown transparent solution (solid content concentration: 4.22%). This was diluted 3 times with purified water to obtain a lactic acid bacteria fermentation product solution.

Production Example 2 Preparation of fermented product (2)
1163 g (solid content concentration: 4.01%) of a lactic acid bacteria fermented product solution was obtained in the same manner as in Production Example 1 except that yak licorice was used in place of Honkaku of Production Example 1.

Production Example 3 Preparation of fermented product (3)
1208 g (solid content concentration 4.13%) of a lactic acid bacteria fermented product solution was obtained in the same manner as in Production Example 1 except that Nikkokisuge was used in place of Honkanzo in Production Example 1.

Production Example 4 Preparation of fermented product (4)
1133 g (solid content concentration 4.31%) of a lactic acid bacteria fermented product solution was obtained in the same manner as in Production Example 1 except that lactic acid bacteria (Leuconostoc lactis) were used instead of Lactobacillus plantarum of Production Example 1.

Production Example 5 Preparation of fermented product (5)
Purified water 1500 g, cellulase 1.5 g and pectinase 1.5 g were mixed with 150 g of Honkan elephant flower parts, extracted for 3 hours at 40 ° C. and subjected to enzymatic degradation, then heated at 80 ° C. for 1 hour, The enzyme was deactivated. Furthermore, the extract suspension obtained here was sterilized by heating. This extract suspension was inoculated with 10 ⁸ cells / mL of yeast (Saccharomyces cerevisiae) and statically cultured at 30 ° C. for 3 days under a nitrogen stream. After completion of the culture, the mixture was sterilized by heating, and the culture broth was filtered to perform deodorization and decoloration treatment to obtain 1108 g of a brown transparent yeast fermented product solution (solid content concentration: 3.91%). This was diluted 3 times with purified water to obtain a yeast fermented product solution.

Production Example 6 Preparation of fermented product (6)
1203 g of yeast fermented product solution (solid content concentration: 3.76%) was obtained in the same manner as in Production Example 5 except that yeast (Candida versachiris) was used instead of Saccharomyces cerevisiae in Production Example 5.

Production Example 7 Preparation of fermented product (7)
1158 g of yeast fermented product solution (solid content concentration: 3.69%) was obtained in the same manner as in Production Example 5 except that yak licorice was used instead of Honkaku of Production Example 5.

Production Example 8 Preparation of fermented product (8)
1120 g (solid content concentration: 3.74%) of a yeast fermented product solution was obtained in the same manner as in Production Example 5 except that Nikkokisuge was used instead of Honkanzo in Production Example 5.

Production Example 9 Preparation of fermented product (9)
Purified water 1500 g, cellulase 1.5 g and pectinase 1.5 g were mixed with 150 g of Honkan elephant flower parts, extracted for 3 hours at 40 ° C. and subjected to enzymatic degradation, then heated at 80 ° C. for 1 hour, The enzyme was deactivated. Furthermore, the extract suspension obtained here was sterilized by heating. The koji mold (Aspergillus oryzae) on the extracted suspension was inoculated 10 ⁸ / mL, and allowed to stand for 3 days incubation at 30 ° C. under a stream of nitrogen. After completion of the culture, the mixture was sterilized by heating, and the culture solution was filtered to perform deodorization and decolorization treatment to obtain 1083 g (solid content concentration: 4.35%) of a brown transparent gonococcal fermentation product solution. This was diluted 3 times with purified water to obtain a koji mold fermented product solution.

Production Example 10 Preparation of fermented product (10)
Purified water 1500 g, cellulase 1.5 g and pectinase 1.5 g were mixed with 150 g of Honkan elephant flower parts, extracted for 3 hours at 40 ° C. and subjected to enzymatic degradation, then heated at 80 ° C. for 1 hour, The enzyme was deactivated. Furthermore, the extract suspension obtained here was sterilized by heating. The extract suspension Bacillus subtilis (Bacillus Natto) were inoculated 10 ⁸ cells / mL in and allowed to stand for 3 days incubation at 30 ° C. under a stream of nitrogen. After completion of the culture, the mixture was sterilized by heating, and the culture solution was filtered to perform deodorization and decolorization treatment to obtain 1233 g (solid content concentration 4.15%) of a brown transparent Bacillus subtilis fermentation product. This was diluted 3 times with purified water to obtain a Bacillus subtilis fermentation product solution.

Production Example 11 Preparation of fermented product (11)
Instead of the flower part of Production Example 1, the whole suspension of lactic acid bacteria fermented solution (solid) was prepared in the same manner as in Production Example 1 except that an extract suspension was prepared using a cut portion of the ground part (including the flower part). A partial concentration of 3.58%).

Production Example 12. Preparation of Extracts 150 g of Honkan elephant flower parts were mixed with 1500 g of purified water, 1.5 g of cellulase and 1.5 g of pectinase, extracted at 40 ° C. for 3 hours, and subjected to enzymatic degradation treatment. The enzyme was inactivated by heating for a period of time. Further, the extract suspension thus obtained was sterilized by heating to obtain a brown transparent solution (solid content concentration: 5.82%). This was diluted 5 times with purified water to obtain an extract solution.

Production Example 13 Preparation of extract After mixing 1500 g of purified water, 1.5 g of cellulase, and 1.5 g of pectinase with 150 g of shredded flower of Aedes albopictus, extraction and enzymatic degradation treatment were performed at 40 ° C. for 3 hours, and then 1 The enzyme was inactivated by heating for a period of time. Furthermore, the extract suspension obtained here was sterilized by heating to obtain a brown transparent solution (solid content concentration: 5.62%). This was diluted 5 times with purified water to obtain an extract solution.

Formulation Example 1 Lotion [Ingredients] part Jojoba oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
Fermented product of Production Example 1 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide appropriate amount Purified water Amount to make 100 parts in total Perfume Appropriate amount

Formulation Example 2 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the fermented product of Production Example 2 was used instead of the fermented product of Production Example 1 included in Formulation Example 1.

Formulation Example 3 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the fermented product of Production Example 3 was used instead of the fermented product of Production Example 1 contained in Formulation Example 1.

Formulation Example 4 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the fermented product of Production Example 4 was used instead of the fermented product of Production Example 1 included in Formulation Example 1.

Formulation Example 5 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the fermented product of Production Example 5 was used instead of the fermented product of Production Example 1 contained in Formulation Example 1.

Formulation Example 6 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the fermented product of Production Example 6 was used instead of the fermented product of Production Example 1 included in Formulation Example 1.

Formulation Example 7 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the fermented product of Production Example 7 was used instead of the fermented product of Production Example 1 included in Formulation Example 1.

Formulation Example 8 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the fermented product of Production Example 8 was used instead of the fermented product of Production Example 1 included in Formulation Example 1.

Formulation Example 9 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the fermented product of Production Example 9 was used instead of the fermented product of Production Example 1 included in Formulation Example 1.

Formulation Example 10 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the fermented product of Production Example 10 was used instead of the fermented product of Production Example 1 included in Formulation Example 1.

Formulation Example 11 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the fermented product of Production Example 11 was used instead of the fermented product of Production Example 1 included in Formulation Example 1.

Formulation Example 12. Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the extract of Production Example 12 was used instead of the fermented product of Production Example 1 included in Formulation Example 1.

Formulation Example 13 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 13 was used instead of the fermented product of Production Example 1 included in Formulation Example 1.

Formulation Example 14. Latex [Ingredients] part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Lotus essential oil 0.025
Polyoxyethylene (20) sorbitan monostearate 1.0
Lipophilic glyceryl stearate 1.0
Hydrogenated soybean lecithin 1.5
Fermented product of Production Example 1 3.0
L-ascorbic acid 2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Water-soluble collagen 0.1
Purified water Total amount of 100 parts Perfume Suitable amount

Formulation Example 15. Emulsion An emulsion was obtained in the same manner as in Formulation Example 14 except that 3.0 parts of the fermented product of Production Example 5 was used instead of 3.0 parts of the fermented product of Production Example 1 included in Formulation Example 14.

Formulation Example 16. Emulsion An emulsion was obtained in the same manner as in Formulation Example 14 except that 2.0 parts of tranexamic acid was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide contained in Formulation Example 14. It was.

Formulation Example 17. Emulsion An emulsion was obtained in the same manner as in Formulation Example 14 except that 3.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide contained in Formulation Example 14. .

Formulation Example 18. Emulsion The emulsion was prepared in the same manner as in Formulation Example 14 except that 5.0 parts of nicotinamide was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide contained in Formulation Example 14. Obtained.

Formulation Example 19. Emulsion [Ingredients] part squalane 3.0
Behenyl alcohol 3.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 1.0
Glycerin fatty acid ester 1.0
Soy lecithin 1.5
Fermented product of Production Example 1 5.0
L-ascorbic acid 2-glucoside 2.0
Potassium hydroxide 0.5
Dipotassium glycyrrhizinate 0.1
Glycerin 3.0
1,3-butylene glycol 2.0
Water-soluble collagen 0.1
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts

Formulation Example 20. Emulsion An emulsion was obtained in the same manner as in Formulation Example 19 except that 1.0 part of tranexamic acid was used instead of 1.0 part of dipotassium glycyrrhizinate contained in Formulation Example 19.

Formulation Example 21. Lotion [Component] part Fermented product of Production Example 2 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Xanthan gum 0.1
Guar gum 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water Amount to make 100 parts in total

Formulation Example 22. Lotion A lotion was obtained in the same manner as in Formulation Example 21, except that 10.0 parts of the fermented product of Production Example 3 was used instead of the fermented product of Production Example 2 among the ingredients of Formulation Example 21.

Formulation Example 23. Essence [Ingredients] part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Hydrolyzed hyaluronic acid solution 0.1
Fermented product of Production Example 4 5.0
Citric acid 0.3
Sodium citrate 0.6
Amount of purified water totaling 100 parts

Example 24. Liquid foundation [ingredient] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
Fermented product of Production Example 6 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount to be 100 parts Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount

Formulation Example 25. Body shampoo [ingredient] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
Fermented product of Production Example 7 5.0
1,3-butylene glycol 2.0
Amount of purified water totaling 100 parts

Formulation Example 26. Hair shampoo [ingredient] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
Citric acid 0.1
Fermented product of Production Example 1 2.0
1,3-butylene glycol 2.0
Amount of purified water totaling 100 parts

Example 27. Hair conditioner [ingredient] part polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
Fermented product of Production Example 5 2.0
1,3-butylene glycol 5.0
Amount of purified water totaling 100 parts

Formulation Example 28. Hair restorer [ingredient] part dipotassium glycyrrhizinate 0.1
Mononitroguaiacol sodium 0.02
Pyridoxine hydrochloride 0.03
l-Menthol 0.8
Japanese cypress raft root extract 0.3
Brown algae extract 0.3
Panax ginseng extract 0.3
Assembly extract 2.0
Fermented product of Production Example 1 5.0
Trimethylglycine 0.5
Lactic acid 0.2
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Polyoxyethylene hydrogenated castor oil 0.4
L-arginine appropriate amount ethanol 20.0
Amount of purified water totaling 100 parts

Formulation Example 29. Hair restorer A hair restorer was obtained in the same manner as in Formulation Example 28 except that 5.0 parts of the fermented product of Production Example 5 was used instead of the fermented product of Production Example 1 contained in Formulation Example 28.

Formulation Example 30. Hair restorer A hair restorer was obtained in the same manner as in Formulation Example 28 except that 5.0 parts of the fermented product of Production Example 9 was used instead of the fermented product of Production Example 1 contained in Formulation Example 28.

Formulation Example 31. Hair restorer A hair restorer was obtained in the same manner as in Formulation Example 28 except that 5.0 parts of the extract of Production Example 12 was used instead of the fermented product of Production Example 1 contained in Formulation Example 28.

Test Example 1 XVII type collagen gene expression evaluation test Normal human epidermal cells were prepared to 6 × 10 ⁵ cells / mL with HuMediaKG2 (Kurabo) containing growth additives, 1 mL was seeded in a φ6 cm dish, 5% CO ₂ , The cells were cultured at 37 ° C under saturated steam. After culturing for 24 hours, a culture solution containing the fermented product of Production Examples 1 to 10 and the extract of Production Examples 12 to 13 as a sample solution The solution was added) and cultured. In addition, as a comparative control, instead of the sample solution, a test group to which a culture solution containing only the PBS (-) solution (the final concentration of PBS (-) with respect to the total amount of the culture solution was adjusted to 1%) was added (control). Ward). After culturing for 24 hours, the cells in each test group were collected with 1 mL of Trizol reagent (Invitrogen). 200 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) is added to the collected cells, stirred and mixed, and then centrifuged at 15,000 rpm, 4 ° C. for 15 minutes with a centrifuge (TOMY / MX-160) Only 400 μL of the aqueous layer was collected. To the recovered aqueous layer, 500 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added, mixed with stirring, and centrifuged at 15,000 rpm at 4 ° C. for 15 minutes to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to totalRNA, stirred and washed, and centrifuged at 15,000 rpm under 4 ° C. for 15 minutes to collect a precipitate. The recovered total RNA was subjected to reverse transcription using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) [manufactured by Takara Bio Inc.]) to synthesize cDNA. Using the synthesized cDNA as a sample, Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.), and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [manufactured by Takara Bio Inc.] Expression of type XVII collagen gene and expression of internal standard β-actin gene were detected. Here, β-actin is one of housekeeping genes (a gene that is expressed in a certain amount in common in many tissues and cells and is always expressed and essential for cell maintenance and proliferation). Since the expression level is always constant, it is used as an internal standard in PCR experiments. As a test result, the expression level of the type XVII collagen gene in each test section was compared when the expression level of β-actin gene was constant. In this test system, the relative value of the expression level of each gene in other test plots when the expression level of each gene in the control plot was set to 100 was determined.

The results of Test Example 1 are shown in Table 1.
[Table 1]

  As shown in Table 1, it was confirmed that the extract and the fermented product according to the present invention had an excellent expression enhancing effect on the type XVII collagen gene.

Test Example 2 Sodium-dependent vitamin C transporter (SVCT) synthesis promoting effect Normal human epidermal melanocytes are prepared to 1 × 10 ⁵ cells / mL with DermaLife (registered trademark) [manufactured by Kurabo Industries Inc.] containing growth additives, 96-well microplate 100 μL each was seeded and cultured at 37 ° C. in 5% carbon dioxide gas and saturated steam. After 24 hours, a culture solution containing the fermented products 1 to 11 of Production Example 1 and the extracts of Production Examples 12 to 13 as sample solutions was further added and further cultured. Here, the sample solution was prepared so that the final concentration as a solution with respect to the culture solution was 1.0% and 2.0%. As a control, a control group was set in which a culture solution containing PBS (−) was added instead of the sample solution. PBS (-) was prepared so as to have final concentrations of 1.0% and 2.0% with respect to the culture medium as a solution in the same manner as the sample solution. After 48 hours, the culture supernatant was removed, 200 μL of PBS (−) was added and removed, and then 50 μL of 10% trichloroacetic acid (Wako Pure Chemical Industries, Ltd.) was added and incubated at cold temperature for 30 minutes. Thereafter, the supernatant was removed. The plate was washed with 100 μL of PBS (−), 50 μL of PBS (−) containing 0.2% Triton-X was added, and incubated at room temperature for 1 hour. After removing the supernatant, 50 μL of PBS (−) containing 8% bovine serum albumin (SIGMA) was added and incubated at room temperature for 2 hours. The supernatant was removed, washed with 100 μL of 0.2% Triton-X-containing PBS (−), 50 μL of anti-SVCT mouse monoclonal antibody (Santa Cruz) was added and incubated at cold temperature for 24 hours. The supernatant was removed, and washing was repeated 3 times using 100 μL of 0.2% Triton-X-containing PBS (−). 50 μL of Alexa Fluor 488 anti-mouse secondary antibody (Life Technologies) was added and incubated at room temperature for 2 hours in the dark. The supernatant was removed, and washing was repeated 3 times using 100 μL of 0.2% Triton-X-containing PBS (−). 100 μL of PBS (−) was added and Ex485 / Em520 was added using a fluorescent plate reader (Dainippon Pharmaceutical Co., Ltd.). The fluorescence intensity was measured. The relative value of the fluorescence intensity with respect to the measured value in the control group was defined as the SVCT synthesis rate (%).

The results of Test Example 2 are shown in Table 2.
[Table 2]

Test Example 3 Lipid peroxide inhibitory effect
0.5 mL ethanol linoleate 1.0 mL, 0.2 M phosphate buffer (pH 7.0) 10 mL and ethanol 9.0 mL were mixed and stirred thoroughly. To this mixed solution, 5.0 mL of the fermented products of Production Examples 1 to 11 and the extracts of Production Examples 12 to 13 as sample solutions were added and stirred sufficiently to prepare sample solutions. Here, as the sample solution, those having two concentrations prepared so that the final concentration (concentration as a solution) of the sample solution with respect to the total amount was 1.0% and 2.0%, respectively, were used. Immediately after the preparation of this sample solution, the samples were left at 40 ° C. for 1 day, 2 days, 5 days, 8 days, 0.1 mL, 4.7% of 75% ethanol, 0.1 mL of 30% ammonium thiocyanate solution, and After adding 0.1 mL of a 3.5% hydrochloric acid solution of 0.02M ferrous chloride and thoroughly mixing and stirring, the absorbance at a wavelength of 500 nm was measured after 3 minutes. Also. As a control, a similar test was performed on a 30% aqueous solution of 1,3-butylene glycol, and the amount of lipid peroxide produced in this test system was expressed as an increase in absorbance at a wavelength of 500 nm after subtracting the value immediately after preparation.

The results of Test Example 3 are shown in Table 3.
[Table 3]

  As shown in Table 3, it was confirmed that the fermented product according to the present invention has an excellent lipid peroxide inhibiting effect.

Claims (5)

  A composition for external application of skin, comprising as an active ingredient a fermented product obtained by fermenting a plant belonging to the genus Hemerocallis or its extract with lactic acid bacteria, koji molds or natto bacteria.   A whitening composition comprising as an active ingredient a fermented product obtained by fermenting a plant belonging to the genus Hemerocallis or an extract thereof in yeast.   A moisturizing composition comprising as an active ingredient a fermented product obtained by fermenting a plant belonging to the genus Hemerocallis or an extract thereof with yeast.   A composition for hair cosmetics comprising as an active ingredient a plant belonging to the genus Hemerocallis or an extract thereof, or a fermented product obtained by fermenting the plant or an extract thereof with lactic acid bacteria, yeast, koji mold or natto bacteria. .   An external composition for skin comprising a water-soluble solvent extract of a plant belonging to the genus Hemerocallis.