JP2007217661A - Method for producing water-soluble xylan-containing polysaccharide and use thereof - Google Patents

Abstract

【Task】
A method for producing a water-soluble xylan-containing polysaccharide useful in food, cosmetics, pharmaceuticals, and general industrial fields.
[Solution]
A plant raw material prepared by pulverization or grinding to have an average particle diameter of 500 μm or less is heated in an alkaline medium of 0.025 to 0.5 N, and from the obtained residue, 0.25 to 2 Water-soluble xylan-containing polysaccharide containing 30% by mass or more of xylose component and 40% or more of component having a molecular weight of 10,000 to 120,000, extracted in 5N alkaline medium and partially hydrolyzed by allowing xylanase to act on the extract A process for producing a water-soluble xylan-containing polysaccharide, characterized in that
[Selection figure] None

Description

  The present invention relates to a method for producing a water-soluble xylan-containing polysaccharide useful in foods, cosmetics, pharmaceuticals, and general industrial fields.

  In recent years, the use of natural extracts instead of synthetic products has been reconsidered due to increasing awareness of people's health and concerns about the safety of chemical substances. The same applies to water-soluble polysaccharides widely used in the food, cosmetic and pharmaceutical industries. Against this background, attempts have been made to produce water-soluble polysaccharides from plant materials.

  In Patent Document 1, a raw material of cereal nuts, bran or hulls is treated with a starch destabilizing step by adding a heat-stable amylase in the presence of hot water, and the destarched raw material is treated under alkaline or acidic conditions. A method for producing water-soluble hemicellulose is described in which a water-soluble hemicellulose fraction-containing liquid obtained in the extraction step is then subjected to a neutralization / desalting step. According to this document, by destarching Nuka and bran in hot water, the extraction time in the subsequent hemicellulose extraction process can be greatly shortened. Since the enzyme is mixed, a removal step is required to obtain a high-purity hemicellulose fraction. In addition, the heat-resistant amylase essential in the process is expensive, and there is a problem that the cost is increased.

  In Patent Document 2, the wheat bran is washed with water to remove water-soluble substances, and then treated with an alkaline aqueous solution of 0.1 to 0.4 N to dissolve a section mainly composed of hemicellulose in the aqueous alkaline solution. A method for extracting and purifying hemicellulose is described which is purified in turn using an ultrafiltration membrane having a molecular weight of 8000-50000 and an ion exchange resin. According to such a method, it is possible to obtain hemicellulose having high purity and high water solubility in a short time. However, since this method separates the water-soluble fraction from the alkali-soluble fraction by ultrafiltration, there is a problem that the extracted alkali-soluble / water-insoluble fraction is lost to some extent.

  Patent Document 3 is characterized in that a arabinoxylan-containing portion of the Gramineae family is heat-treated at a temperature of 100 to 145 ° C. and a pressure of 1 to 4 atm in the presence of moisture, and then a plant cell wall-degrading enzyme is allowed to act on this. A method for preparing a water-soluble arabinoxylan is described. According to such a method, it is possible to extract and separate high-purity water-soluble arabinoxylan with water alone. However, since this method requires a high temperature of 100 ° C. or higher and a high pressure of 1 atmosphere or more, expensive equipment and enormous energy are required for extraction. In addition, arabinoxylan is denatured when the plant material is subjected to high temperature and high pressure.

  Patent Document 4 describes a water-soluble polysaccharide obtained by germinating plant seeds belonging to the family Gramineae, the saccharification process, and by allowing an enzyme preparation having xylan-degrading enzyme activity to act on the residue obtained by saccharification and filtration. ing. According to this method, it is possible to obtain a water-soluble polysaccharide having a high emulsifying power. However, in this method, the alkali extraction step is one step, and the water-soluble polysaccharide and impurities are extracted at the same time. Therefore, in order to obtain a highly pure emulsifiable polysaccharide from the extract, the purification step becomes complicated. There was a problem. On the other hand, the present invention removes impurities and extracts polysaccharides by multi-stage treatment of plant raw materials in media having different alkali concentrations. Therefore, the water-soluble polysaccharide described in the document is essential. Is different. In addition, the water-soluble polysaccharide obtained by the method of this document has a xylose / arabinose weight ratio of 2.1 / 1 to 1.9 / 1 and needs to contain a large amount of arabinose as a constituent component. Further, the water-soluble polysaccharide needs to contain a large amount of protein. Since the water-soluble xylan-containing polysaccharide of the present invention has a high xylose content and does not need to contain a large amount of arabinose and protein, it is completely different from the water-soluble polysaccharide of this document also in terms of constituent components. is there.

  Patent Document 5 discloses xylose, arabinose, or galactose, which is obtained by subjecting the hull to acid treatment at pH 1.0 to 3.0 and then wet heat treatment at 120 to 150 ° C. in a method for obtaining water-soluble saccharide from cereal hull containing hemicellulose. A method for producing a water-soluble polysaccharide comprising one or more sugars selected from as a constituent sugar is disclosed. According to this method, water-soluble hemicellulose can be obtained in high yield from cereal hulls. However, in the extraction process, a high temperature treatment of 100 ° C. or higher is required, and the equipment cost and energy cost are increased. In addition, there is a problem that arabinoxylan decomposes not a little when treating plant raw materials at high temperatures under acidic conditions.

  Patent Document 6 discloses a method in which a plant fiber raw material is alkali-extracted and the extract is treated with xylanase to obtain a partially decomposed product of hemicellulose. However, since generally available xylanases are acidic and active, a large amount of acid is required to neutralize the alkali used for extraction. Furthermore, there is a drawback that a great burden is required to remove a large amount of salt generated by neutralization. In addition, when alkali xylanase is used, neutralization from strong alkali to weak alkali is essential, and alkali xylanase is generally difficult to obtain. Furthermore, this method has a drawback that the yield is extremely low. In addition, this publication does alkali extraction in one stage and does not describe the usefulness of multistage treatment with alkali. In the present invention, the yield and purity are improved by two-stage extraction, or an alkali treatment is carried out in a specific range, and the xylanase is reacted with the residue instead of the extract. This is completely different in that xylanase is reacted with an alkali-extracted extract.

Therefore, a plant raw material having a specific average particle size is subjected to multistage treatment in alkaline media having different concentrations, and a xylan-containing polysaccharide dissolved in the medium is partially hydrolyzed with xylanase, resulting in a high xylose content. , A water-soluble xylan-containing polysaccharide controlled to a specific molecular weight and excellent in emulsification and thickening, and a specific plant material is washed with dilute alkalinity, and the residue is washed with alkaline hydrogen peroxide and / or as required. A method for producing a water-soluble xylan-containing polysaccharide having a high xylose content obtained by treating with sodium hypochlorite and reacting the treated product with xylanase and having a low coloration controlled to a specific molecular weight, and these The use of was not known.
Japanese Patent Publication No.6-111764 Japanese Patent No. 2688509 Japanese Patent No. 3079115 Japanese Patent Laid-Open No. 10-237107 Japanese Patent Laid-Open No. 11-113600 Japanese Patent No. 2792601

  An object of this invention is to provide the manufacturing method of water-soluble xylan containing polysaccharide useful in a foodstuff, cosmetics, a pharmaceutical, and a general industrial field | area.

In order to solve the above-mentioned problems, the inventors of the present invention processed a plant raw material having a specific average particle diameter in multiple stages in alkaline media having different concentrations, and partially dissolved the xylan-containing polysaccharide dissolved in the medium with xylanase. The present inventors have found a water-soluble xylan-containing polysaccharide that is obtained by hydrolysis and has a high xylose content, is controlled to a specific molecular weight, and is excellent in emulsification and viscosity. Furthermore, the present inventors improve the reactivity with xylanase by washing a specific plant raw material with dilute alkalinity, and if necessary, the residue is treated with alkaline hydrogen peroxide and / or sodium hypochlorite and It has been found that the reactivity with xylanase can be further improved simultaneously with decolorization by treating with ozone. It was discovered that by reacting the treated product with xylanase, a water-soluble xylan-containing composition having a low degree of coloring can be obtained even from a highly colored plant material, and the present invention has been achieved. That is, the present invention is as follows.
(1) A plant raw material prepared by pulverization or grinding to an average particle size of 500 μm or less is heated in an alkaline medium of 0.025 to 0.5 N, and from the resulting residue, 0. Extracted in an alkaline medium of 25 to 2.5N, and partially hydrolyzed by allowing xylanase to act on the extract, 30% by mass or more of xylose component, 40% or more of component having a molecular weight of 10,000 to 120,000 A method for producing a water-soluble xylan-containing polysaccharide comprising obtaining a water-soluble xylan-containing polysaccharide (2) A plant raw material is washed in an alkaline medium of 0.025 to 0.5 N, and after alkali treatment A xylanase is reacted with a solid substance, a water-soluble xylose having a xylose component of 30% by mass or more, a molecular weight of 10,000 to 120,000 of 40% or more, and a coloring degree of 1.0 or less. Soluble xylan-containing polysaccharide preparation method characterized by obtaining the down-containing polysaccharide.
(3) The plant raw material is washed with an alkaline medium of 0.025 to 0.5N, the washed plant raw material is treated with alkaline hydrogen peroxide and / or sodium hypochlorite and / or ozone, and the treatment is performed. A water-soluble xylan-containing polysaccharide having a xylose component of 30% by mass or more, a molecular weight of 10,000 to 120,000 having a molecular weight of 40% or more, and a coloring degree of 1.0 or less is obtained by reacting a product with xylanase. The manufacturing method of the water-soluble xylan containing polysaccharide characterized by the above-mentioned.
(4) The water-soluble xylan-containing polysaccharide according to any one of (1) to (3), wherein the plant raw material is at least one selected from cottonseed husk, bagasse, and corn core. Production method.
(5) The water-soluble xylan-containing polysaccharide according to any one of (1) to (4) above and one or more food materials, and optionally surfactants, thickeners, gelling agents, fragrances, specific gravity Aqueous solution, emulsion, aqueous dispersion, paste, gel form prepared by emulsifying, dispersing, dissolving, or mixing one or more selected from regulators, preservatives, pH regulators, chelating agents, and colorants together with an aqueous medium. And a water-soluble xylan-containing polysaccharide composition having any form of powder and granules obtained by drying them.

  The xylan-containing polysaccharide obtained by the present invention has a high xylose content, excellent emulsifiability and viscosity increase, and low coloration.

  The preferred embodiments of the present invention will be specifically described below. The water-soluble xylan-containing polysaccharide as used in the present invention is a polysaccharide containing xylose as a constituent sugar and containing xylose converted to a polysaccharide by β-glucoside bond, and is contained in a plant raw material. Is partially hydrolyzed with xylanase.

The xylose component referred to in the present invention is a polysaccharide containing xylose itself and xylose unit “C ₅ H ₁₀ O ₅ ” in the water-soluble xylan-containing polysaccharide. These contents in the water-soluble xylan-containing polysaccharide are expressed by quantifying xylose in the polysaccharide.

  The raw material for the water-soluble xylan-containing polysaccharide of the present invention needs to use a vegetable raw material. The plant raw material referred to here is derived from a plant and includes components constituting the plant. The components constituting the plant contained in the raw material are, for example, polysaccharides such as cellulose and hemicellulose, oligosaccharides such as xylose, glucose, arabinose, mannose, and galactose, or saccharide sugars. Among the above plant raw materials, cotton seed, bagasse or corn is preferably used in order to obtain a water-soluble xylan-containing polysaccharide in a high yield. As used herein, cottonseed is a perennial seed belonging to the mallow family, and as long as the object of the present invention can be achieved, the raw materials used may include cotton, cotton, petals, linters, oils, etc. . Among cotton seeds, it is preferable to use cottonseed husk as a raw material because the cottonseed husk has a high hemicellulose content as a raw material for the water-soluble xylan-containing polysaccharide. In addition, the bagasse of the present invention is a generic name for components other than sugar among sugarcane components, and is industrially a squeezed rice cake obtained by collecting sugar, and is used if the object of the present invention can be achieved. Ingredients may include skin, piss, sugar and the like. The corn of the present invention is not particularly limited in its cultivar, production area, site, etc., but it is preferable to use a core or hull with a high hemicellulose content.

  The plant raw material of the present invention is preferably pulverized to an average particle size of 500 μm or less. By using a plant raw material having an average particle size of 500 μm or less, alkaline washing, alkaline hydrogen peroxide / sodium hypochlorite treatment, enzyme treatment becomes more efficient, and the xylose content of the water-soluble xylan-containing polysaccharide, and This is preferable because the yield is improved. The average particle size here is a volume-frequency particle size distribution measured by dispersing the sample in water using a light scattering particle size distribution meter (trade name LA-910, manufactured by Horiba, Ltd.) This is expressed as a particle size having a cumulative volume of 50%. In order to further improve the above-mentioned xylose content and yield, the average particle size is more preferably 300 μm or less, and particularly preferably 200 μm or less.

  The pulverization method of the plant raw material of the present invention is not particularly limited as long as it is a known method, but examples include grinding, pulverization, classification using a sieve, cyclone, centrifugation using a centrifuge, and the like. They can be used alone or in combination of two or more. In addition, the above-described treatment may be wet or dry, and even if each obtained by wet is mixed before enzymatic decomposition, or each obtained by dry is mixed before enzymatic decomposition, You may combine what was obtained by the wet type or the dry type.

  For example, when processing vegetable raw materials in a wet process, the average particle size of the vegetable raw materials can be easily obtained by subjecting a dispersion containing 1 to 99% of the vegetable raw materials and the medium to known grinding and pulverization. Can be adjusted. The medium used here is not particularly limited. For example, water, methanol, ethanol, n-propyl alcohol, isopropyl alcohol, butyl alcohol, 2-methylbutyl alcohol, benzyl alcohol and other alcohols, pentane, hexane, Examples thereof include hydrocarbons such as heptane and cyclohexane, and ketones such as acetone and ethyl methyl ketone. In particular, organic solvents are preferably used in the process of producing pharmaceuticals, foods and their additives. “Pharmaceutical Additives Encyclopedia” (published by Yakuji Nippo Co., Ltd.), “Japan Pharmacopoeia”, “Food These are classified as solvents in the “Additives Official Document” (all published by Yodogawa Shoten). Water and organic solvents can be used alone or in combination of two or more, and once dispersed in one medium, the medium is removed and dispersed in a different medium. Also good. Moreover, you may swell with an alkali and implement grinding and grinding | pulverization.

  As a grinding method, a stirring blade such as a one-way rotary type such as a portable mixer, a three-dimensional mixer, a side mixer, a multi-axis rotary type, a reciprocating inversion type, a vertical movement type, a rotation + vertical movement type, and a pipe type is used. Milling method, jet-type stirring milling method such as line mixer, grinding method using high shear homogenizer, high-pressure homogenizer, ultrasonic homogenizer, rotary extrusion milling method using kneader, roll mill, ball mill, vibration Any method such as a grinding method combining compaction and shearing, such as a ball mill or a bead mill, may be used, or a method combining them may be used.

  As the pulverization method, screen-type pulverization methods such as screen mill and hammer mill, blade-rotating shear screen-type pulverization method such as flash mill, air-flow-type pulverization method such as jet mill, compaction such as roll mill, ball mill, vibration ball mill, and bead mill. Any method such as a pulverization method combining shearing, a blade stirring type pulverization method, or the like may be used, or a method combining them may be used.

  The water-soluble xylan-containing polysaccharide of the present invention is prepared by heating a refined vegetable raw material in an alkaline medium of 0.025 to 0.5 N to remove impurities, and using the obtained residue as a raw material. It is obtained by extraction in an alkaline medium of 0.25 to 2.5N. The impurities referred to here are components other than sugars such as lignin, tannin, lipids and proteins derived from plant raw materials. In the present invention, in order to increase the xylose content of the water-soluble xylan-containing polysaccharide, These must be removed from the sexual raw material. The alkali concentration in removing impurities is freely set within the scope of the present invention so that a high content of water-soluble xylan-containing polysaccharide xylose can be obtained. In order to reduce it, the alkali concentration is preferably 0.10 to 0.45N, particularly preferably 0.2 to 0.4N. Furthermore, the temperature and time for removing impurities are also appropriately adjusted so that a high content of water-soluble xylan-containing polysaccharide xylose is obtained. The impurity removal temperature is preferably 60 to 140 ° C., preferably 80 to 120 ° C. is more preferable. The treatment time is preferably 10 to 120 minutes, more preferably 30 to 100 minutes, and particularly preferably 40 to 90 minutes.

  Moreover, in this invention, it is preferable to collect | recover the plant raw material from which the impurity was removed, and to extract in a 0.25-2.5N alkaline medium. The alkali concentration in the extraction is freely adjusted within the above range so that the xylose content of the water-soluble xylan-containing polysaccharide and the yield of the water-soluble xylan-containing polysaccharide are improved. In the above range, the higher the alkali concentration, the higher the xylose content and the yield of the water-soluble xylan-containing polysaccharide. However, when the alkali concentration exceeds the above range, the extracted water-soluble xylan-containing polysaccharide is denatured and collected. This is not preferable because the rate decreases. The preferred alkali concentration range is 0.5 to 2.2N, more preferably 1.0 to 2.0N. In addition, the temperature and time in the extraction are appropriately adjusted so as to improve the xylose content and yield of the water-soluble xylan-containing polysaccharide, but the extraction temperature is preferably 60 to 120 ° C., 80 to 100 ° C. is more preferable. The extraction time is preferably 10 to 120 minutes, more preferably 30 to 100 minutes, and particularly preferably 60 to 90 minutes.

  The water-soluble xylan-containing polysaccharide of the present invention is obtained by washing plant raw materials in an alkaline medium of 0.025 to 0.5N, removing impurities, and then decomposing with xylanase using the obtained residue as a raw material. Can also be obtained. The impurities referred to here are components other than sugars such as lignin, tannin, lipids and proteins derived from plant raw materials. In the present invention, in order to increase the xylose content of the water-soluble xylan-containing polysaccharide, In order to increase the reactivity, it is necessary to once wash the plant raw material with a dilute alkali. In addition, the alkali concentration in the washing is freely set within the scope of the present invention so that a high content of water-soluble xylan-containing polysaccharide xylose can be obtained, but it reduces impurity removal and xylan loss. Therefore, the alkali concentration is preferably 0.10 to 0.45 N, and particularly preferably 0.2 to 0.4 N. Furthermore, the temperature and time for washing are also adjusted as appropriate so that a high content of xylose in the water-soluble xylan-containing composition can be obtained. The impurity removal temperature is preferably 60 to 140 ° C., and preferably 80 to 120. ° C is more preferred. The treatment time is preferably 10 to 240 minutes, more preferably 30 to 180 minutes, and particularly preferably 60 to 120 minutes.

  Moreover, in this invention, it is preferable to collect the plant raw material which carried out the alkali washing | cleaning, and to perform an alkaline hydrogen peroxide and / or sodium hypochlorite treatment, and / or. The alkali concentration in the alkaline hydrogen peroxide treatment is not particularly limited as long as active species of hydrogen peroxide is generated, but is preferably 0.001N to 5N. More preferably, it is 0.01-2N, Most preferably, it is 0.1-0.5N. If it is 0.001 N or less, the decolorization effect is not sufficient, and it is feared that the yield of the finally obtained water-soluble xylan-containing polysaccharide decreases. On the other hand, if it is 5N or more, the active species of hydrogen peroxide is generated at a time and may not be used effectively. As the amount of hydrogen peroxide used increases, the yield of the finally obtained water-soluble xylan-containing polysaccharide tends to be improved. It is preferable to use 0.001 to 2.0 mass, preferably 0.01 to 1.0 mass, more preferably 0.03 to 0.5 mass.

  When using sodium hypochlorite, it is not necessary to use an alkali, and it may be used as it is. The amount of sodium hypochlorite used is preferably 1 ppm to 5% in terms of effective chlorine concentration.

In the case of performing the ozone treatment, either treatment with ozone water or blowing treatment into the washed plant material suspension may be used. Although there is no restriction | limiting in particular in ozone concentration, Since an effect is acquired in a short time, it is preferable. For example, in the case of blowing, 50 to 200 g / Nm ³ is preferable.

  The temperature and time of alkaline hydrogen peroxide and / or sodium hypochlorite treatment and / or ozone treatment are also appropriately adjusted so that the xylose content and yield of the water-soluble xylan-containing polysaccharide are improved. As a processing temperature, 25-100 degreeC is preferable and 50-80 degreeC is more preferable. The treatment time is preferably 60 to 240 minutes and more preferably 90 to 180 minutes in the hydrogen peroxide treatment. In the case of sodium hypochlorite, 60 to 90 minutes are preferable.

  In the above-described cleaning and alkaline hydrogen peroxide and / or sodium hypochlorite treatment, the alkaline substance used is not particularly limited, and examples thereof include sodium hydroxide, potassium hydroxide, calcium hydroxide, magnesium hydroxide, water. Those used in the process of manufacturing foods such as barium oxide and ammonia, cosmetics, pharmaceuticals and their additives are preferred. "Pharmaceutical Additives Encyclopedia" (published by Yakuji Nippo Co., Ltd.), "Japan Pharmacopoeia", Examples include those described in the “Food Additive Official Document” and “Cosmetics Additive Standard” (both published by Yodogawa Shoten). Alkaline substances can be used alone or in combination of two or more, and once dissolved or dispersed in one medium, the medium is removed and dissolved in a different medium. Also good. One or more of sodium hydroxide, potassium hydroxide, and calcium hydroxide are preferably used because they are easily available at low cost.

  In the above-described washing and alkaline hydrogen peroxide treatment, when the plant raw material is dispersed in the medium for treatment, 1 to 90 parts by weight of the plant raw material is dispersed with respect to 100 parts by mass of the medium. Preferred in terms. More preferably, it is 1-40 mass parts, More preferably, it is 5-30 mass parts of plant raw materials. Further, it is preferable to carry out the above treatment with stirring or shearing because the solid-liquid composition is kept uniform during the treatment, so that the removal of impurities and the yield of the water-soluble xylan-containing polysaccharide are improved. The stirring or shearing preferably has a circumferential speed (circumferential speed of the rotary blade) of 0.1 to 10 m / sec. More preferably, it is 0.5-5 m / sec.

  The water-soluble xylan-containing polysaccharide of the present invention is obtained by washing plant raw materials in a dilute alkaline medium, removing impurities, and further extracting with an alkaline medium, or reacting with a xylanase by dilute alkali treatment in a specific concentration range. The residue is treated with alkaline hydrogen peroxide and / or sodium hypochlorite as necessary to improve decolorization and further reactivity with xylanase. It can be obtained by allowing xylanase to act on these extracts and / or processed products to hydrolyze the polysaccharides contained in the composition. The xylanase referred to in the present invention is a general term for enzymes having an action of hydrolyzing xylan-based substances, and any xylanase referred to in the present invention is included in the xylanase as long as it has xylan decomposing activity.

  Examples of xylanase enzyme sources include, for example, purified xylanase-producing cells themselves, purified enzymes that accumulate or secrete xylanase-producing bacteria, and formulated purified enzymes together with additives such as excipients and stabilizers. Can be mentioned. In the case of a xylanase preparation, the additive added thereto is not particularly limited, and the dosage form may be any of powder, granule, liquid and the like. The origin of xylanase is not particularly limited, and examples of microorganisms that produce known xylanases include Basilis polymyxa, Bassilus poumillius, Bassilus subtilis, Cellvibrio bulbtus, Clostridium, Rumen bacteris, Aspergillus niger, Aspergillus olizae, Cephalosporia sacchari, Ceratocytosis paradoxa, Chetomium globosam, Cysosporum lignorum, Coniophora ebella, Fomes igiarius, Fomesmaruginatus, Gloeophyllum saepiarium, Helminthoisporium, Humicola insolens, Irpex lacteus, Lentites saepiaria, Malbranchea pullchea, Manulius lacrymans, Marulius silvester, Myrothesium verrucaria, Penicillium funuculosum, Penicillium verrufulosom, Penicillium wortimunni, Polyporus betulinus, Shizpphyllum communue, Trametes Produced by bacteria described in "Cellulase" (published by Kodansha Scientific (1987)) and "Encyclopedia of Cellulose" (published by Asakura Shoten (2000)) such as ersicolor, Tricoderma konningii, Tricoderma violet, Tricoderma sp Although xylanase can be mentioned, as long as it has an action of degrading xylanase, it is not limited to those derived from the above-mentioned known bacteria, and enzymes derived from novel bacteria are also included in the xylanase of the present invention.

  The hydrolysis characteristics of the enzyme are not particularly limited, but in order to suppress the generation of monosaccharides and oligosaccharides during hydrolysis and to improve the yield of water-soluble polysaccharides, the hydrolysis characteristics include endo (endo). ) Type is preferred.

  The method for partial hydrolysis may be a known method and is not particularly limited. For example, a xylan-based substance is dissolved or suspended in an aqueous medium as a substrate, xylanase is added, A method of heating while stirring or shaking is mentioned.

  In the above method, the reaction conditions such as dissolution, suspension, stirring method, xylanase / substrate addition method / addition order, concentration thereof, etc. are appropriately adjusted so that the yield of water-soluble xylan-containing polysaccharide and xylose content are improved. Is. In this case, the pH, temperature, and treatment time of the reaction solution may be within the range where the enzyme is not inactivated. Generally, when the reaction is performed at normal pressure, the temperature is 5 to 95 ° C., and the pH is It may be in the range of 1 to 11 and may be decomposed under pressure to improve the reaction rate and selectivity. For example, when using a xylanase derived from Tricoderderm (for example, trade name Sumiteam X manufactured by Shin Nippon Chemical Industry Co., Ltd.), the optimum reaction conditions for partial hydrolysis are pH 3.0 to 7.0, 30 to 60 ° C. It is preferable to process for 1 to 24 hours.

The xylose content of the water-soluble xylan-containing polysaccharide of the present invention is 30% by mass or more. The xylose content of the water-soluble xylan-containing polysaccharide is such that the enzymatic decomposition solution is adjusted to pH 4.5, the water-insoluble component is separated by centrifugation, the supernatant is dried, and the solid content obtained here is 20 mg. High-performance liquid chromatography (column: manufactured by Shimadzu Corporation, trade name: Asahipak NH ₂ P-50, high-performance liquid chromatography: manufactured by Shimadzu Corporation) was treated with 2N trifluoroacetic acid at 95 ° C. for 2 hours and eluted in the medium. Product name SCL-10A type, column temperature: 40 ° C., moving bed: acetonitrile / water = 75/25 (volume ratio) circulation rate: 1 mL / min. Sample solution: 10 μL). The higher the xylose content, the higher the xylose content in the water-soluble xylan-containing polysaccharide and the better the emulsifiability and viscosity increase of the water-soluble xylan-containing polysaccharide. More preferably, it is 35 mass% or more, More preferably, it is 40 mass% or more, Most preferably, it is 50 mass% or more. The higher the xylose content, the higher the xylan purity. Therefore, the upper limit is not particularly set, but the range obtained by a simple operation is 99% by mass or less.

  The water-soluble xylan-containing polysaccharide of the present invention preferably contains 40% or more of a component having a molecular weight of 10,000 to 120,000. More preferably, it is 20000-110000, More preferably, it is 30000-70000. The molecular weight here is obtained by dissolving the obtained water-soluble xylan-containing polysaccharide in water and gel permeation chromatography (column: product name G3000PWXL, manufactured by Tosoh Corporation, high performance liquid chromatography: product name SCL-10A manufactured by Shimadzu Corporation). Mold, column temperature: 70 ° C., moving bed: water, circulation rate: 0.7 mL / min. Sample solution: 10 μL), and measured in terms of polyethylene glycol. When the molecular weight is measured by another measurement method, the value may be greatly different. However, if the molecular weight measured under the above conditions is within the range of the present invention, it is the water-soluble xylan-containing polysaccharide of the present invention. The term “40% or more” as used herein means that the area of the peak having a peak top has 40% or more of the total peak area in a molecular weight range of 10,000 to 120,000 expressed in the chromatogram. When the components in the above molecular weight range are contained in an amount of 40% or more, impurities other than oligosaccharide components and saccharides are small, water-soluble polysaccharides are increased, and the water-soluble xylan-containing polysaccharides are excellent in emulsifiability and viscosity. This content is more preferably 50% or more, and particularly preferably 60% or more. The higher the content, the better the above-mentioned effect, but the more preferable content is 99% or less as a range of the content achieved by a simple operation.

  The coloring degree of the present invention means an absorbance at 420 nm when a 1% aqueous solution of a water-soluble xylan-containing polysaccharide is used. When the coloring degree exceeds 1.0, for example, when it is added to a beverage or the like, the color tone of the beverage may be greatly changed. Therefore, the coloring degree is preferably 1.0 or less. The lower limit of the coloring degree is not particularly limited, but is substantially 0.0001 or more as a substantially obtained source.

  The water-soluble xylan-containing polysaccharide obtained by the above enzymatic decomposition can be subjected to purification treatment such as decolorization, desalting, and enzyme removal, if necessary. The purification method is not particularly limited as long as it is a known method. For example, activated carbon treatment, ion exchange resin treatment, chromatography treatment, microfiltration, ultrafiltration, reverse osmosis filtration and other filtration treatment, concentration, or poor solvent / salt Precipitation / crystallization treatment by oxygen, oxygen bleach, chlorine bleach and the like may be used, and these may be used alone or in combination of two or more.

  The water-soluble xylan-containing polysaccharide of the present invention preferably has an emulsification rate of 80% or more. The emulsification rate mentioned here represents the amount of emulsification when 5 parts by mass of polysaccharide is added to a mixture of water / oil mass ratio = 45/50 and emulsified. This emulsification rate is obtained by dissolving 5 parts by mass of water-soluble xylan in 45 parts by mass of water at 80 ° C., and further adding 50 parts by mass of rapeseed oil (trade name canola oil manufactured by Nissin Eulio), and then in a warm bath at 80 ° C. Using a homogenizer (trade name TK homogenizer manufactured by Tokushu Kika Co., Ltd.), the mixture is mixed at 8000 rpm for 5 minutes, brought to room temperature, and expressed as a volume percentage of the emulsified layer in the total mixed solution. A higher emulsification rate is preferable because the emulsifying power of the water-soluble xylan-containing polysaccharide is excellent. A more preferable range is 90%, and 95% or more is particularly preferable. The completely emulsified state is most preferable, and the upper limit of the emulsification rate is 100%.

  The water-soluble xylan-containing polysaccharide of the present invention preferably has a viscosity increase rate of 20000% or less. The thickening rate here is the ratio of the viscosity of an aqueous solution in which 10% by mass of the water-soluble xylan-containing polysaccharide is dissolved to the viscosity of the aqueous solution in which 5% by mass of the water-soluble xylan-containing polysaccharide is dissolved. This viscosity is the static viscosity of the aqueous solution measured with a B-type viscometer at 25 ° C. and 60 rpm. More preferably, it is 10,000% or less, Most preferably, it is 1000% or less. The higher the value of the thickening rate, the higher the viscosity with respect to the amount of the water-soluble xylan-containing polysaccharide added, which affects the texture of beverages and the like. The lower limit is not particularly limited, but is 110% or more as a realistic range of the thickening rate.

  Next, the water-soluble xylan-containing polysaccharide composition of the present invention will be described. The water-soluble xylan-containing polysaccharide composition of the present invention is one or more types in which the water-soluble xylan-containing polysaccharide of the present invention is selected from food materials, cosmetic materials, medicinal medicinal ingredients, or additives used in them. It is preferable to be an aqueous solution, an emulsion, an aqueous dispersion, a paste, or a gel food / cosmetic / pharmaceutical. In addition, the composition may be a dry product such as powder, granule, or molded product.

  The above-mentioned water-soluble xylan-containing polysaccharide composition has a water-soluble xylan-containing polysaccharide content of the present invention of 0.1 to 99.9%, and other ingredients include food materials / cosmetic materials / medicinal medicinal ingredients, Or it is preferable that 1 or more types chosen from the additive used by them are included. The composition containing the water-soluble xylan-containing polysaccharide can be processed by a known method such as dissolution, mixing, dispersion, granulation, melting / solidification, compression, and drying.

  The water-soluble xylan-containing polysaccharide composition of the present invention contains the water-soluble xylan-containing polysaccharide of the present invention, so that the emulsifiability and viscosity are improved in the dissolution, dispersion, and mixing of the above functional components. Since the water-soluble xylan-containing polysaccharide of the present invention is safely extracted from plant raw materials, it is preferable to use the composition as a food, cosmetic or pharmaceutical product.

  The water-soluble xylan-containing polysaccharide composition of the present invention is one or more types in which the water-soluble xylan-containing polysaccharide of the present invention is selected from food materials, cosmetic materials, medicinal medicinal ingredients, or additives used in them. The functional components used herein include, for example, the following.

  In the present invention, functional ingredients include food materials, cosmetic materials, pharmaceutical medicinal ingredients, agricultural chemical ingredients, fertilizer ingredients, feed ingredients, pigments, fragrances, metals, ceramics, catalysts, or excipients, disintegrants, binders, fluids Agents, lubricants, flavoring agents, flavoring agents, coloring agents, sweeteners, solvents, fats and oils, surfactants, thickeners, gelling agents, etc., powders, crystals, oils , Liquid, semi-solid, etc., for example, “Pharmaceutical Additives Encyclopedia” (published by Yakuji Nippo Co., Ltd.), “Food Additives Official Document”, “Cosmetics Additives Official Document” (published by Yodogawa Shoten) Can be used. In addition, they may be coated for various purposes. These functional components may be used alone or in combination. The addition amount of the active ingredient is preferably 0.01% to 99.9%.

  In particular, when this functional ingredient is a medicinal medicinal ingredient, if the added amount of the active ingredient is less than 0.01%, a therapeutically effective amount cannot be secured, and if it exceeds 99.9%, the water-solubility of the present invention The xylan-containing polysaccharide is less than 0.1%, which is not preferable because the effects of the present invention cannot be obtained.

  For example, anti-pyretic analgesics, antihypnotics, drowsiness preventives, antipruritics, pediatric analgesics, stomachic drugs, antacids, digestives, cardiotonic drugs, arrhythmic drugs, antihypertensives, vasodilators , Diuretics, anti-ulcer drugs, intestinal drugs, osteoporosis treatments, antitussive expectorants, anti-asthma drugs, antibacterial agents, frequent urination drugs, nourishing tonics, vitamins, etc. . The medicinal component can be used alone or in combination of two or more.

  Additives include excipients, disintegrants, binders, lubricants, fluidizers, flavoring agents, fragrances, colorants, solvents, fats and oils, thickeners, surfactants, foaming agents, gelling agents, etc. Is included.

  Excipients include starch acrylate, L-aspartic acid, aminoethylsulfonic acid, aminoacetic acid, candy (powder), gum arabic, gum arabic powder, alginic acid, sodium alginate, pregelatinized starch, inositol, ethylcellulose, ethylene Vinyl acetate copolymer, sodium chloride, olive oil, kaolin, cacao butter, casein, fructose, pumice grains, carmellose, carmellose sodium, hydrous silicon dioxide, dry yeast, dry water, aluminum oxide gel, dry sodium sulfate, dry magnesium sulfate, agar , Agar powder, xylitol, citric acid, sodium citrate, disodium citrate, glycerin, calcium glycerophosphate, sodium gluconate, L-glutamine, clay, clay granules, croscarmellose Lithium, cross polyvinyl pyrrolidone, magnesium aluminate silicate, calcium silicate, magnesium silicate, light anhydrous silicic acid, light liquid paraffin, cinnamon powder, crystalline cellulose, crystalline cellulose / carmellose sodium, crystalline cellulose (grain) , Synthetic aluminum silicate, synthetic hydrotalcite, sesame oil, wheat flour, wheat starch, wheat germ powder, rice, rice starch, potassium acetate, calcium acetate, cellulose acetate phthalate, safflower oil, white beeswax, zinc oxide, titanium oxide , Magnesium oxide, β-cyclodextrin, dihydroxyaluminum aminoacetate, 2,6-di-butyl-4-methylphenol, dimethylpolysiloxane, tartaric acid, potassium hydrogen tartrate, baked gypsum, sucrose fatty acid ester Steal, magnesium hydroxide alumina, aluminum hydroxide / gel, aluminum hydroxide / sodium bicarbonate coprecipitate, magnesium hydroxide, scrawan, stearyl alcohol, stearic acid, calcium stearate, polyoxyl stearate, magnesium stearate, Sterotex HM , Purified gelatin, purified shellac, purified sucrose, purified sucrose spherical granules, cetostearyl alcohol, cetopolyethylene glycol, gelatin, sorbitan fatty acid ester, D-sorbitol, tricalcium phosphate, soybean oil, soybean unsaponifiable matter, soybean lecithin, skim milk powder , Talc, ammonium carbonate, calcium carbonate, magnesium carbonate, neutral anhydrous sodium sulfate, low-substituted hydroxypropylcellulose, dextran, dextrin, natural silica Aluminum, corn starch, tragacanth powder, silicon dioxide, calcium lactate, lactose, white petrolatum, sucrose, sucrose / starch spherical granule, powdered green leaf extract, naked malt green juice dried powder, honey, paraffin, potato starch, semi-digested body Starch, human serum albumin, hydroxypropyl starch, hydroxypropylcellulose, hydroxypropylmethylcellulose phthalate, phytic acid, glucose, glucose hydrate, partially pregelatinized starch, pullulan, propylene glycol, powdered reduced maltose starch syrup, powdered cellulose, pectin, bentonite , Sodium polyacrylate, polyoxyethylene alkyl ether, polyoxyethylene hydrogenated castor oil, polyoxyethylene polyoxypropylene glycol, police Sodium lensulfonate, polyvinyl acetal diethylaminoacetate, polyethylene glycol, maltitol, maltose, D-mannitol, water candy, isopropyl myristate, anhydrous lactose, anhydrous calcium hydrogen phosphate, anhydrous calcium phosphate granule, magnesium aluminate metasilicate, Methylcellulose, cottonseed powder, cottonseed oil, mole, aluminum monostearate, glyceryl monostearate, sorbitan monostearate, medicinal charcoal, peanut oil, aluminum sulfate, calcium sulfate, granular corn starch, liquid paraffin, dl-malic acid, Calcium phosphate-calcium hydrogen phosphate, calcium hydrogen phosphate, calcium hydrogen phosphate granule, sodium hydrogen phosphate, potassium dihydrogen phosphate, calcium dihydrogen phosphate, diphosphate Those classified as excipients in the “Pharmaceutical Additives Encyclopedia” (published by Yakuji Nippo Co., Ltd.), “Food Additives Official”, “Cosmetics Official Addendum” (published by Yodogawa Shoten) such as sodium hydrogen Even if it is used alone, two or more kinds can be used in combination.

  Disintegrants include croscarmellose sodium, carmellose, carmellose calcium, carmellose sodium, celluloses such as low-substituted hydroxypropyl cellulose, carboxymethyl starch sodium, hydroxypropyl starch, rice starch, wheat starch, corn starch, potato “Pharmaceutical Additives Encyclopedia” (published by Yakuji Nippo Co., Ltd.) such as starches, starches such as partially pregelatinized starch, synthetic polymers such as cross polyvinylpyrrolidone and cross polyvinylpyrrolidone copolymer, And those classified as disintegrants (issued by Yodogawa Shoten). Even if it uses individually by 1 type chosen from the above, it is also free to use 2 or more types together.

  As binders, sugars such as sucrose, glucose, lactose, fructose, sugar alcohols such as mannitol, xylitol, maltitol, erythritol, sorbitol, gelatin, pullulan, carrageenan, locust bean gum, agar, gluconannan, xanthan gum, tamarind Water-soluble polysaccharides such as gum, pectin, sodium alginate, gum arabic, etc., celluloses such as crystalline cellulose, powdered cellulose, hydroxypropylcellulose, methylcellulose, starches such as pregelatinized starch, starch paste, polyvinylpyrrolidone, carboxyvinyl polymer, Synthetic polymers such as polyvinyl alcohol, inorganic compounds such as calcium hydrogen phosphate, calcium carbonate, synthetic hydrotalcite, magnesium aluminate silicate etc. "(Yakujinipposha Co., Ltd. issued)," food additive official statement ", mention may be made of what is classified as a binding agent to the" cosmetic additives official Manual "(Hirokawa Shoten). Even if it uses individually by 1 type chosen from the above, it is also free to use 2 or more types together.

  As fluidizing agents, “Pharmaceutical Additives Encyclopedia” (published by Yakuji Nippo Co., Ltd.), “Food Additives Official”, “Cosmetics Additives Official” Can be listed as a fluidizing agent in the book (published by Yodogawa Shoten). Even if it uses individually by 1 type chosen from the above, it is also free to use 2 or more types together.

  Lubricants include: “Pharmaceutical Additives Encyclopedia” (published by Yakuji Nippo Co., Ltd.) such as magnesium stearate, calcium stearate, stearic acid, sucrose fatty acid ester, talc, etc. What is classified as a lubricant in the “Additive Official Document” (published by Yodogawa Shoten) can be mentioned. Even if it uses individually by 1 type chosen from the above, it is also free to use 2 or more types together.

  As a corrigent, "Pharmaceutical Additives Encyclopedia" such as glutamic acid, fumaric acid, succinic acid, citric acid, sodium citrate, tartaric acid, malic acid, ascorbic acid, sodium chloride, 1-menthol, etc. (published by Yakuji Nippo Co., Ltd.) ), “Food Additives Official Document” and “Cosmetics Additive Official Document” (issued by Yodogawa Shoten). Even if it uses individually by 1 type chosen from the above, it is also free to use 2 or more types together.

  As perfumes, oils such as orange, vanilla, strawberry, yogurt, menthol, fennel oil, cinnamon oil, spruce oil, mint oil, etc., “Pharmaceutical Additives Encyclopedia” such as green tea powder (published by Yakuji Nippo Co., Ltd.), Examples include those classified as flavoring agents and fragrances in the “Food Additives Official Document” and “Cosmetics Additive Official Document” (published by Yodogawa Shoten). Even if it uses individually by 1 type chosen from the above, it is also free to use 2 or more types together.

  As coloring agents, “Drugs for Food Additives” such as Food Red No. 3, Food Yellow No. 5, Food Blue No. 1, etc., copper chlorofin sodium, titanium oxide, riboflavin, etc. (published by Yakuji Nippo Co., Ltd.) , “Food additive official document”, “Cosmetic additive official document” (published by Yodogawa Shoten), and those classified as colorants. Even if it uses individually by 1 type chosen from the above, it is also free to use 2 or more types together.

  Sweeteners include aspartame, saccharin, dipotassium glycyrrhizinate, stevia, maltose, maltitol, starch syrup, and amacha powder “Pharmaceutical Additives Encyclopedia” (published by Yakuji Nippo Co., Ltd.) And those classified as sweeteners in “Cosmetics Official Additives” (published by Yodogawa Shoten). Even if it uses individually by 1 type chosen from the above, it is also free to use 2 or more types together.

  The solvent is not particularly limited as long as it is used in pharmaceuticals. For example, a “pharmaceutical additive encyclopedia” such as alcohols such as methanol and ethanol, and ketones such as acetone (Pharmaceutical Daily Inc.) Issued), “Food Additives Official”, “Cosmetics Official” (published by Yodogawa Shoten) are listed as solvents, and even if they are used alone, two or more must be used in combination Is also free.

  Examples of fats and oils include stearic acid monoglyceride, stearic acid triglyceride, stearic acid sucrose ester, paraffins such as liquid paraffin, hardened oils such as carnauba wax and hardened castor oil, castor oil, stearic acid, stearyl alcohol, polyethylene glycol, etc. Listed in the “Pharmaceutical Additives Encyclopedia” (published by Yakuji Nippo Co., Ltd.), “Food Additives Official Document”, and “Cosmetics Additives Official Document” (published by Yodogawa Shoten). Even if it uses, it is also free to use 2 or more types together.

  As a thickener, for example, “pharmaceutical additive encyclopedia” such as hydroxypropylcellulose, hydroxypropylmethylcellulose, polyacrylic acid, carboxyvinyl polymer, polyethylene glycol, polyvinyl alcohol, polyvinylpyrrolidone, methylcellulose, ethylcellulose, gum arabic, starch paste, etc. (Published by Yakuji Nippo Co., Ltd.), “Food Additives Official Document”, “Cosmetics Additive Official Document” (published by Yodogawa Shoten), and even if it is used alone, Two or more kinds can be used in combination.

  Examples of the surfactant include phospholipid, glycerin fatty acid ester, polyethylene glycol fatty acid ester, sorbitan fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene cetyl ether, polyoxyethylene stearyl ether, polyoxyethylene nonylphenyl ether, Polyoxyethylene polyoxypropylene glycol, polyoxyethylene sorbitan sun monolaurate, polysorbate, sorbitan monooleate, monostearate glyceride, monooxyethylene sorbitan monopalmitate, monooxyethylene sorbitan monostearate, polyoxymonooleate "Pharmaceutical Additives Encyclopedia" such as ethylene sorbitan, sorbitan monopalmitate, sodium lauryl sulfate ) Issued), “Food Additives Official Document”, “Cosmetics Additives Official Document” (published by Yodogawa Shoten) are listed as surfactants. They can also be used together.

  Examples of the gelling agent include animal gelling agents such as gelatin, agar, xanthan gum, guar gum, gum arabic, curdlan, locust bean gum, carboxymethyl cellulose, hydroxyethyl cellulose, cellulose, microcrystalline cellulose, microcrystalline cellulose and the like. "Pharmaceutical Additives Dictionary" (published by Yakuji Nippo Co., Ltd.), "Food Additives Official Document", "Cosmetics Additives Official Document" (published by Yodogawa Shoten) Are classified as gelling agents, and they can be used alone or in combination of two or more.

  Examples of physiologically active food materials include coenzyme Q10, hyaluronic acid, ceramide, L-carnitine, L-ornithine, GABA, various vitamins such as vitamin C and vitamin E, minerals such as zinc and iron, docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), medium chain fatty acids, oligosaccharides such as emulsified oligosaccharides and galactooligosaccharides, soy isoflavone, astaxanthin, α-lipoic acid, β-glucan, nattokinase, lycopene, Examples include catechins and derivatives thereof.

  A food / cosmetic / pharmaceutical comprising the water-soluble xylan-containing polysaccharide of the present invention and one or more functional ingredients selected from food materials, cosmetic materials, medicinal medicinal ingredients, or additives used in the following. Although a manufacturing method is described, the effect of the present invention is not limited to the following method.

  The method for adding each component is not particularly limited as long as it is a commonly used method. 1) Even if the water-soluble xylan-containing polysaccharide and the functional component are added simultaneously and mixed / dispersed / emulsified, 2) water-soluble 3) Mixing / dispersing two or more functional components in advance after mixing / dispersing / emulsifying another functional xylan-containing polysaccharide and a specific functional component / After emulsification, a water-soluble xylan-containing polysaccharide may be added, mixed / dispersed / emulsified, or a combination of these addition methods. When the functional components to be added are solutions, suspensions, and emulsions, dispersion of component concentrations in the final product is reduced by adopting a method of spraying them on water-soluble xylan-containing polysaccharides or other additives. This is preferable.

  The devices used here are small suction transport devices, pneumatic transport devices, bucket conveyors, pressure-feed transport devices, vacuum conveyors, vibratory quantitative feeders, sprays, funnels, etc. May be. The mixing method of each component is not particularly limited as long as it is a normal method, but a container rotary mixer such as a V type, W type, double cone type, container tack type mixer, or high-speed stirring is used. A stirring mixer such as a mold, a universal stirring type, a ribbon type, a pug type, and a Nauta type mixer, a high-speed fluid mixer, a drum mixer, and a fluidized bed mixer may be used. A shaker mixer such as a shaker can also be used.

  The dispersion method is not particularly limited as long as it is a normal dispersion method, but it is a one-way rotation type such as a portable mixer, a three-dimensional mixer, a side mixer, a multi-axis rotation type, a reciprocating reversal type, a vertical movement type, and a rotation + up and down direction. Mixing method using stirring blades such as mobile type, pipe type, jet type stirring and mixing method such as line mixer, gas blowing type stirring and mixing method, high shear homogenizer, high pressure homogenizer, ultrasonic homogenizer, etc. The method may also be a container-shaking mixing method using a shaker, or a combination of these methods.

  Further, in the above mixing and dispersion, there is no particular limitation on the order of adding an aqueous medium to which a surfactant, a thickener, and a gelling agent are added to water or a water / organic solvent as necessary. Even if other functional components are added to the water-soluble xylan-containing polysaccharide in advance after the aqueous medium is added and dissolved / dispersed / emulsified, 2) the aqueous medium is added in advance to the functional components and dissolved / dispersed / Even after emulsification, the water-soluble xylan-containing polysaccharide may be added. 3) The water-soluble xylan-containing polysaccharide and the functional component may be mixed / dispersed / emulsified in advance, and then an aqueous medium may be added. The composition mixed / dispersed / emulsified by the above method may be once dried, and the resulting dried product may be mixed / dispersed / emulsified in a medium and / or a functional component, or a combination thereof. Each liquid or semi-solid food / cosmetic / pharmaceutical such as aqueous dispersion, paste, emulsion, gel, etc. obtained here is dried as necessary and subjected to processing such as granulation, coating, molding and the like.

  The water-soluble xylan-containing polysaccharide of the present invention is excellent in thickening, film-forming properties, and binding properties, so that it can be used for thickening other functional components or used as a shape-retaining agent. It may be used as a coating agent or a binder during granulation.

  The granulation / coating method is not particularly limited as long as it is a known method, but either a stirring method or a fluidized bed method may be used, or a method combining them may be used. Examples of the agitation granulator include a one-way rotary type such as a portable mixer, a three-dimensional mixer, and a side mixer, a multi-axis rotary type, a reciprocating reversal type, a vertical movement type, a rotation + vertical movement type agitator, and a fluidized bed type. An upper spray type, a central spray type, a lower spray type, a combined stirring type, a central can jet type, a Wurster type and the like can be mentioned. Moreover, you may give the dry granulation which uses a roller compactor.

  Regarding the coating, a granulated product may be obtained in advance, and a known coating may be applied thereto, or after coating, another coating may be applied to form a multilayer. As a spraying method of the coating agent, a method of spraying an active ingredient solution / dispersion using a pressure nozzle, a two-fluid nozzle, a four-fluid nozzle, a rotating disk, an ultrasonic nozzle, etc., an active ingredient solution / dispersion from a tubular nozzle Any method of dropping may be used. When the active ingredient solution / dispersion is added, even if the active ingredient is layered or coated on the surface of the water-soluble xylan-containing polysaccharide particles, it is supported inside the water-soluble xylan-containing polysaccharide particles. Alternatively, water-soluble xylan-containing polysaccharide particles or a mixture of the water-soluble xylan-containing polysaccharide and other functional components may be granulated in a matrix using a functional component solution / dispersion as a binder. The effect is the same whether the layering or coating is wet or dry.

  The water-soluble xylan-containing polysaccharide of the present invention is excellent in retention of liquid components, and when it is used as a core particle, aggregation of particles during layering and coating can be suppressed.

  In addition, when the active ingredient is a solution, suspension, or emulsion, a mixture of a water-soluble xylan-containing polysaccharide and other additives is used as a carrier, and the active ingredient solution, suspension, or emulsion is dipped. A method of immersing and holding the active ingredient can be taken. Although depending on the conditions such as the component type and concentration, even the liquid dipping method such as dipping is excellent in that the uniformity of the component concentration is practically maintained and the process is simple compared to the above spraying.

  Furthermore, when the active ingredient is a solution, suspension, or emulsion, after immersing the active ingredient solution, suspension, or emulsion in a mixture of a water-soluble xylan-containing polysaccharide and other additives as a carrier The dispersion may be spray-dried to form a composite.

  The mixture of the water-soluble xylan-containing polysaccharide and other additives before and after the addition of the functional component solution / dispersion is in the form of an agglomerated granulated product even when the individual unit particles are dispersed individually. May be.

  The molding method is not particularly limited as long as it is a commonly performed method, but a mold may be used, and a known molding method such as compression, melting, injection, rolling, etc. can be applied, and a method combining these But you can. Examples of molding machines used here include compression molding machines, melt molding machines, injection molding machines, and rolling molding machines. Confectionery / cosmetics / pharmaceutical molding machines, cooked rice molding machines, compressed molding machines, packaging machines Well-known molding machines, such as a rice cake manufacturing apparatus, a dumpling / wrapping molding machine, and a foundation substrate compression molding machine, can be used. In particular, regarding compression molding, a method of compression molding into a desired shape using a mold, or a method of pre-compressing into a sheet shape and then cleaving into a desired shape may be used. As the compression molding machine, for example, a roller press such as a hydrostatic press, a briquetting roller press, a smooth roller press, a compressor such as a single punch tablet press, or a rotary tablet press can be used. . The molded product obtained by the above-described method may be coated by a known method. When the molded product is a tablet, it may be coated with sugar to form a sugar-coated tablet. In particular, the water-soluble xylan-containing polysaccharide of the present invention may be blended in the sugar coating layer in the sugar-coated tablet.

  Examples of foods containing the water-soluble xylan-containing polysaccharide of the present invention include, for example, gels such as jelly, pudding, and yogurt, mayonnaise, dressings, sauces, sauces, soups, processed vegetables, seasonings, and curry , Retort food such as hayashi, meat sauce, stew, soup, chilled food, hamburger, bacon, sausage, salami sausage, hams and other livestock products, rice cake, chikuwa, fish ham / sausage, fried rice cakes, bread , Raw noodles, dry noodles, macaroni, spaghetti, Chinese bun peel, cake mix, premix, white sauce, dumplings, spring rolls and other wheat processed foods, curry, sauce, soup, boiled canned, jam, etc. , Bottles, candy, caramel, troches, tablet confectionery, chocolate, biscuits, cookies, gum, rice crackers Pastries such as Japanese and Western confectionery, Western confectionery, snack confectionery, confectionery such as sugar confectionery, pudding, fried foods, croquettes, dumplings, Chinese buns, vegetable paste, meat mince, fruit paste, seafood paste, etc. It is. In addition, there are dairy products such as ice cream, ice milk, lact ice, whipped cream, condensed milk, butter, yogurt, cheese and white sauce, and processed oils and fats such as margarine, fat spread and shortening. In addition, carbonated drinks such as cola, carbonated drinks, alcohol drinks, fruit drinks mixed with dairy products, fruit juice or drinks containing fruits, milk drinks, coffee, milk, soy milk, cocoa milk, fruit milk, yogurt, etc. May be used for tea beverages such as lactic acid / milky beverages, sencha, oolong tea, matcha tea, black tea, and the like.

  Examples of cosmetics containing the water-soluble xylan-containing polysaccharide of the present invention include, for example, aqueous solutions, emulsions, creams, pastes, lotions, foams, gels, powders, cosmetic gels, cosmetic liquids, emulsions, creams, etc. Basic cosmetics, cleansing, body shampoo, deodorant, soap, facial cleanser, toothpaste, shaving cream, after-shave lotion, and other cosmetics, base makeup, lipsticks, blusher, foundations, powders, eye makeup, etc. Make-up cosmetics, hair cosmetics, shampoos, rinses, treatments, hair dyes, hair styling agents, hair restorers, etc. hair cosmetics, sunscreen cosmetics, baby cosmetics, natural cosmetics, bath preparations, slimming, hypoallergenic cosmetics, perfume・ Used for colons, nail cosmetics, aroma cosmetics, etc. It may be.

  The water-soluble xylan-containing polysaccharide obtained in the present invention is found in water-soluble dietary fiber, improves the intestinal environment, suppresses blood sugar concentration, blood insulin concentration increase, blood cholesterol reduction, body fat percentage Because various physiological activities such as reduction, bowel movement / stool odor improvement, and anti-tactile properties can be expected, in addition to the above normal food applications, it is used as a physiologically active substance in applications such as functional foods, health foods, diet foods, etc. Also good.

Examples of the present invention will be described below, but the present invention is not limited to the following examples.
[Example 1]
Cotton husk (Okamura Oil Co., Ltd., brand name Hal Blanc) was dry-pulverized with an ultracentrifugal crusher (trade name: ZM100, product name: ZM100, 14,000 rpm) with a screen set with an aperture of 0.25 mm. The opening was changed to 0.12 mm and pulverized in the same manner. Table 1 shows the average particle diameter of the pulverized cottonseed shell. The obtained refined cottonseed husk was suspended in a 0.25N aqueous sodium hydroxide solution at a solid content of 10% by mass, and after removing impurities while shaking at a rate of 90 rpm in a 95 ° C. warm bath, The residue was recovered by filtration, and the residue was suspended in a 1.25N aqueous sodium hydroxide solution with a solid content of 10% by mass, and extracted for 2 hours while shaking at 90 rpm in a 60 ° C. warm bath. After removing the residue from the alkaline medium solution by filtration, the solution was neutralized, and a water-insoluble alkaline extract fraction was obtained by centrifugation. Table 1 shows the results of drying the alkali extract and measuring the xylose content and yield.
[Example 2]
Cottonseed husk (trade name Halbran, manufactured by Okamura Oil Co., Ltd.) is used as a 20% by mass aqueous suspension, and 200 g of the suspension is introduced into a high-speed shearing homogenizer (trade name: Excel Auto Homogenizer) and rotated at 15,000 rpm for 30 minutes. Processed and refined. Table 1 shows the average particle diameter of the cotton seed shell after the treatment. The cottonseed shell was treated with alkali in the same manner as in Example 1 to remove impurities and extract. Table 1 shows the results of drying the alkaline extract and measuring the xylose content and yield.
[Example 3]
Cottonseed husk (trade name Hull Blanc, manufactured by Okamura Oil Co., Ltd.) is made into a 20% by mass aqueous suspension, and 200 g of the suspension is introduced into a high-speed shearing homogenizer (trade name: Excel Auto Homogenizer) and rotated at 15,000 rpm for 120 minutes. Processed and refined. Table 1 shows the average particle diameter of the cotton seed shell after the treatment. The cottonseed shell was treated with alkali in the same manner as in Example 1 to remove impurities and extract. Table 1 shows the results of drying the alkaline extract and measuring the xylose content and yield.
[Example 4]
Bagasse (manufactured by Kyuyo Sugar) was refined in the same manner as in Example 1. Table 1 shows the average particle size of the refined product. The bagasse was treated with alkali in the same manner as in Example 1 to remove impurities and extract. Table 1 shows the results of drying the alkaline extract and measuring the xylose content and yield.
[Comparative Example 1]
Cotton husk (trade name Hull Blanc, manufactured by Okamura Oil Co., Ltd.) was used as it was without being refined, and was alkali-treated by the method of Example 1. Table 1 shows the results of drying the alkaline extract and measuring the xylose content and yield.
[Comparative Example 2]
Bagasse (manufactured by Kyuyo Sugar Co., Ltd.) was used as it was without being refined, and alkali-treated by the method of Example 1. Table 1 shows the results of drying the alkaline extract and measuring the xylose content and yield.
[Examples 5 to 8, Comparative Examples 3 and 4]
50 mM acetic acid / sodium acetate buffer in which the alkaline extracts obtained in Examples 1 to 4 and Comparative Examples 1 and 2 were used as substrates, and 1/100 amount of commercially available xylanase (Sumiteam AS manufactured by Shin Nippon Chemical Co., Ltd.) was added to the substrates. Partial hydrolysis was performed at 55 ° C. for 4 hours while shaking at 90 rpm in the liquid. The residue in the obtained aqueous suspension was precipitated by centrifugation at 5000 G, and the supernatant was collected. The collected supernatant was concentrated under reduced pressure of 40 torr at 70 ° C. to a volume ratio of 10 times, and ethanol was added so that the mass ratio of water and ethanol was 30/70. Was dried to obtain a water-soluble xylan-containing polysaccharide. Table 1 shows the xylose content and yield of the obtained water-soluble xylan-containing polysaccharide.

  Table 1 shows the xylose content and yield of the extract and the enzyme degradation product when using refined raw materials for untreated cottonseed husk and bagasse. By miniaturizing the raw material within the range of the present invention, the xylose content and yield of the enzymatic degradation product were improved.

  Table 1 shows the rate of thickening of the water-soluble xylan-containing polysaccharides obtained in Examples 5, 7, and 8. Since the viscosity of the water-soluble xylan-containing polysaccharide obtained in the examples increases depending on the amount added, it can be used as a thickener.

[Example 9]
Using the water-soluble xylan-containing polysaccharide obtained in Example 7, 5 parts by mass of water-soluble xylan was dissolved in 45 parts by mass of water at 80 ° C., and 50 rapeseed oil (trade name canola oil, manufactured by Nisshin Eulio) was added. After the addition, the mixture was emulsified in a warm bath at 80 ° C. with a homogenizer (trade name TK homogenizer manufactured by Tokushu Kika) at 8000 rpm for 5 minutes. This is introduced into a 100 mL glass mesh cylinder and the emulsification rate after standing for 5 hours, 1 week, 2 weeks is shown in Table 2.
[Comparative Example 5]
In Example 9, instead of the water-soluble xylan-containing polysaccharide, a commercially available xylo-oligosaccharide (manufactured by Suntory, trade name: xylo-oligo 70, component content of 0 to 10 k of average molecular weight) was evaluated in the same manner. The results are shown in Table 2.
[Comparative Example 6]
In place of the water-soluble xylan, a commercially available gum arabic (trade name IRX40693 manufactured by Colloid Naturel) was used and evaluated in the same manner. The results are shown in Table 2.

Table 2 compares the emulsifying power of the water-soluble xylan-containing polysaccharide of the present invention, commercially available xylooligosaccharides, and gum arabic, which is an existing emulsifying polysaccharide. The water-soluble xylan of the present invention has high emulsifiability with respect to xylooligosaccharides by controlling the xylose content and molecular weight within a specific range. Moreover, compared with gum arabic, it showed high emulsification stability.
[Comparative Example 7]
A water-soluble xylan-containing polysaccharide was hydrolyzed with hydrochloric acid to prepare a water-soluble xylan having a molecular weight of 10 to 120 K and a component having a molecular weight of 15%, and was similarly evaluated. The results are shown in Table 2.

  Table 2 shows the emulsifying power of the water-soluble xylan-containing polysaccharide of the present invention, a commercially available xylo-oligosaccharide, and gum arabic which is an existing emulsifying polysaccharide, and the water-soluble xylan-containing polysaccharide not controlled in the molecular weight range of the present invention. Is a comparison. The water-soluble xylan of the present invention has high emulsifiability with respect to xylooligosaccharides by controlling the xylose content and molecular weight within a specific range. Moreover, compared with gum arabic, it showed high emulsification stability. Moreover, long-term emulsification stability was not obtained when the component having a molecular weight of 10 to 120 k was outside the scope of the present invention.

[Example 10]
A high-speed mixer (Excel Auto) was prepared by adding water to 7% by mass of the water-soluble xylan-containing polysaccharide of Example 7, 7% by mass of orange oil (manufactured by Kishida Chemical Co., Ltd.), 2% by mass of damar resin (manufactured by colloidal natural) and 100% by mass The mixture was treated with a homogenizer at 15 000 rpm for 15 minutes, and then the emulsion subjected to a 2-pass treatment at 240 bar with a high-pressure homogenizer (trade name Manton Gorin Homo, manufactured by Niroso Abbey) was spray-dried (trade name, spray dryer SD-9, manufactured by Tokyo Rika Co., Ltd.).

The obtained powder was added to 2 parts by mass with respect to a buffer adjusted to pH 4.0 with acetic acid and sodium acetate, dispersed with a high-speed mixer in the same manner as described above, and then introduced into a glass graduated cylinder for 1 month. Stored at room temperature. As a result of observing the emulsion after storage, oil-off and sediment were not observed, and the emulsion was stable. Further, when the average diameters of the oil droplets before and after storage were compared, almost no increase in the oil droplet diameter was observed (0.83 μm before storage → 0.88 μm after storage).
[Example 11]
200 g of cottonseed husk (trade name Halblanc manufactured by Okamura Oil Co., Ltd.) was suspended in 800 g of a 0.25N aqueous sodium hydroxide solution and washed by stirring at a rate of 200 rpm in a 95 ° C. warm bath. The residue was recovered by filtration, the residue was suspended in a 0.25N aqueous sodium hydroxide solution with a solid content of 20% by mass, 20 ml of 35% hydrogen peroxide was added in a 60 ° C. warm bath, and the mixture was stirred at 200 rpm. However, it was processed for 2 hours. A residue was obtained by filtration, and the residue was washed until the pH of the filtrate was 10 or less. The obtained residue was adjusted to a solid content of 20% by mass, and acetic acid was added until the pH reached 4.7. Thereto was added 0.5 g of xylanase (Sumiteam X, manufactured by Shin Nippon Chemical Co., Ltd.), heated to 55 ° C., and stirred at 500 rpm for 2 hours. The reaction solution was treated at 80 ° C. for 1 hour to inactivate the enzyme, concentrated, crystallized and dried to obtain 14.3 g of a water-soluble xylan-containing polysaccharide. This water-soluble xylan-containing polysaccharide had a coloring degree of 0.63, a xylose component of 65.6%, and a component having a molecular weight of 10,000 to 120,000 of 41.7%.
[Example 12]
20 g of corn cob meal (manufactured by Nippon Fermented Feed) was suspended in 160 g of a 0.25N aqueous sodium hydroxide solution and washed in a 95 ° C. warm bath for 2 hours. A residue was obtained by filtration, and the residue was washed until the pH of the filtrate was 10 or less. The obtained residue was adjusted to a solid content of 10% by mass, and acetic acid was added until the pH reached 4.5. Thereto was added 0.05 g of xylanase (Sumiteam X, manufactured by Shin Nippon Chemical Co., Ltd.), heated to 55 ° C., and shaken at 150 rpm for 2 hours. The reaction solution was treated at 80 ° C. for 1 hour to deactivate the enzyme, concentrated, crystallized, and dried to obtain 1.1 g of a water-soluble xylan-containing polysaccharide. This water-soluble xylan-containing polysaccharide has a coloring degree of 0.13, a xylose component of 46.2%, a molecular weight of 10,000 to 120,000, a component of 87.0%, a molecular weight of 20,000 to 110,000, 61.1%, and 30,000 to 70000. The component was 48%.
[Example 13]
200 g of cottonseed husk (trade name Halblanc manufactured by Okamura Oil Co., Ltd.) was suspended in 800 g of a 0.25N aqueous sodium hydroxide solution and washed by stirring at a rate of 200 rpm in a 95 ° C. warm bath. The residue was collected by filtration, and the residue was suspended in a 0.25N aqueous sodium hydroxide solution with a solid content of 20% by mass, and treated for 2 hours while blowing ozone of 100 g / Nm ³ and stirring at 200 rpm. A residue was obtained by filtration, and the residue was washed until the pH of the filtrate was 10 or less. The obtained residue was adjusted to a solid content of 20% by mass, and acetic acid was added until the pH reached 4.7. Thereto was added 0.5 g of xylanase (Sumiteam AS manufactured by Shin Nippon Chemical Co., Ltd.), heated to 55 ° C., and stirred at 500 rpm for 2 hours. The reaction solution was treated at 80 ° C. for 1 hour to inactivate the enzyme, concentrated, crystallized and dried to obtain 14.3 g of a water-soluble xylan-containing polysaccharide. This water-soluble xylan-containing polysaccharide had a coloring degree of 0.44, a xylose component of 52.1%, and a component having a molecular weight of 10,000 to 120,000 of 47.6%.
[Comparative Example 8]
200 g of cottonseed husk (trade name Halblanc manufactured by Okamura Oil Co., Ltd.) was suspended in 800 g of a 0.25N aqueous sodium hydroxide solution and washed by stirring at a rate of 200 rpm in a 95 ° C. warm bath. Without performing the hydrogen peroxide treatment of Example 11, a residue was obtained by filtration, and the residue was washed until the pH of the filtrate was 10 or less. The obtained residue was adjusted to a solid content of 20% by mass, and acetic acid was added until the pH reached 4.7. Thereto was added 0.5 g of xylanase (Sumiteam AS manufactured by Shin Nippon Chemical Co., Ltd.), heated to 55 ° C., and stirred at 500 rpm for 2 hours. The reaction solution was treated at 80 ° C. for 1 hour to deactivate the enzyme, concentrated, crystallized, and dried to obtain 1.3 g of a water-soluble xylan-containing polysaccharide. Since the hydrogen peroxide treatment was not performed, the degree of coloring was as large as 1.43, and the yield was extremely low.
[Comparative Example 9]
200 ml of 50 mM acetate buffer (pH 4.5) was added to 20 g of bagasse (manufactured by Kyuyo Sugar Co., Ltd.). Thereto was added 0.05 g of xylanase (Sumiteam X, manufactured by Shin Nippon Chemical Co., Ltd.), heated to 55 ° C., and shaken at 150 rpm for 2 hours. The reaction solution was treated at 80 ° C. for 1 hour to deactivate the enzyme, concentrated, crystallized, and dried to obtain 0.1 g of a water-soluble xylan-containing polysaccharide. The xylose component of this water-soluble xylan-containing polysaccharide was 15%. Since impurities were not removed by alkali, the reactivity with xylanase was extremely low, and the water-soluble xylan-containing polysaccharide of the present invention could not be obtained.

Claims (5)

  A plant raw material prepared by pulverization or grinding to have an average particle diameter of 500 μm or less is heated in an alkaline medium of 0.025 to 0.5 N, and from the obtained residue, 0.25 to 2 Water-soluble xylan-containing polysaccharide containing 30% by mass or more of xylose component and 40% or more of component having a molecular weight of 10,000 to 120,000, extracted in 5N alkaline medium and partially hydrolyzed by allowing xylanase to act on the extract A process for producing a water-soluble xylan-containing polysaccharide, characterized in that   The plant raw material is washed in an alkaline medium of 0.025 to 0.5N, the xylanase is reacted with the solid after the alkali treatment, and 40 components having a xylose component of 30% by mass or more and a molecular weight of 10,000 to 120,000 are obtained. %. A method for producing a water-soluble xylan-containing polysaccharide having a coloring degree of 1.0 or less.   The plant material is washed with an alkaline medium of 0.025 to 0.5 N, the washed plant material is treated with alkaline hydrogen peroxide and / or sodium hypochlorite and / or ozone, and the treated product is treated with xylanase To obtain a water-soluble xylan-containing polysaccharide having a xylose component of 30% by mass or more, a molecular weight of 10,000 to 120,000 of 40% or more, and a coloring degree of 1.0 or less. A method for producing a water-soluble xylan-containing polysaccharide.   The method for producing a water-soluble xylan-containing polysaccharide according to any one of claims 1 to 3, wherein the plant raw material is at least one selected from cottonseed husk, bagasse and corn core.   The water-soluble xylan-containing polysaccharide according to any one of claims 1 to 4 and at least one food material, if necessary, a surfactant, a thickener, a gelling agent, a fragrance, a specific gravity adjusting agent, a preservative, An aqueous solution, emulsion, aqueous dispersion, paste, gel, and the like produced by emulsifying, dispersing, dissolving, or mixing together with an aqueous medium with at least one selected from a pH adjusting agent, a chelating agent, and a colorant, and drying these A water-soluble xylan-containing polysaccharide composition having any form of powder and granule obtained in this way.