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HUP0101961A2 - Az FSH-béta-gén módosítása homológ rekombinációval - Google Patents

Az FSH-béta-gén módosítása homológ rekombinációval

Info

Publication number
HUP0101961A2
HUP0101961A2 HU0101961A HUP0101961A HUP0101961A2 HU P0101961 A2 HUP0101961 A2 HU P0101961A2 HU 0101961 A HU0101961 A HU 0101961A HU P0101961 A HUP0101961 A HU P0101961A HU P0101961 A2 HUP0101961 A2 HU P0101961A2
Authority
HU
Hungary
Prior art keywords
fshb
gene
expression
dna constructs
homologous recombination
Prior art date
Application number
HU0101961A
Other languages
English (en)
Inventor
Michael W. Heartlein
Richard F. Selden
Douglas A. Treco
Original Assignee
Transkaryotic Therapies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transkaryotic Therapies, Inc. filed Critical Transkaryotic Therapies, Inc.
Publication of HUP0101961A2 publication Critical patent/HUP0101961A2/hu
Publication of HUP0101961A3 publication Critical patent/HUP0101961A3/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/06—Drugs for disorders of the endocrine system of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/24—Drugs for disorders of the endocrine system of the sex hormones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Endocrinology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • Reproductive Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Public Health (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A találmány tárgyát olyan DNS-konstrukciók képezik, melyek emlőssejtekben, homológ rekombinációval a sejt genomjába integrálódva, azendogén FSHb-gén expressziójának megváltozását idézik elő, és amelyekaz FSHb-gén 5'-végi nem kódoló szekvenciájának legalább 50 egymástfolyamatosan követő nukleotidját magában foglaló célzószekvenciát éstranszkripciós szabályozó szekvenciát tartalmaznak. A találmánytárgyát képezik továbbá a találmány szerinti DNS-konstrukciókat vagyrészeiket tartalmazó izolált nukleinsav-molekulák, valamint a DNS-konstrukciókkal végrehajtott stabil transzfekció útján homológrekombinációval módosított sejtek. A találmány tárgyát képezik atalálmány szerinti DNS-konstrukciók alkalmazásán alapuló eljárások isaz endogén FSHb-gén expressziójának módosítására, az FSHb-géngazdaszervezetekbe történő bejuttatására és az FSHb-proteinelőállítására. A találmány szerinti megoldás alkalmas FSHb-protein invitro előállítására, az FSHb génterápiával történő bejuttatására -például infertilitás kezelése vagy fertilitás növelésére céljából,humán vagy állati szervezetben -, valamint egyéb, az FSHb-expresszióval kapcsolatos rendellenes állapotok kezelésére ésmegelőzésére. Ó
HU0101961A 1998-05-07 1999-05-05 Modifying the expression of the fsh beta gene by homologous recombination HUP0101961A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8466398P 1998-05-07 1998-05-07
PCT/US1999/009795 WO1999057263A1 (en) 1998-05-07 1999-05-05 Modifying the expression of the fsh beta gene by homologous recombination

Publications (2)

Publication Number Publication Date
HUP0101961A2 true HUP0101961A2 (hu) 2001-10-28
HUP0101961A3 HUP0101961A3 (en) 2003-08-28

Family

ID=22186422

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0101961A HUP0101961A3 (en) 1998-05-07 1999-05-05 Modifying the expression of the fsh beta gene by homologous recombination

Country Status (18)

Country Link
US (2) US6200778B1 (hu)
EP (1) EP1075514A1 (hu)
JP (1) JP2002513566A (hu)
KR (1) KR20010052279A (hu)
CN (1) CN1308673A (hu)
AR (1) AR016262A1 (hu)
AU (1) AU766620B2 (hu)
CA (1) CA2328506A1 (hu)
CZ (1) CZ299611B6 (hu)
HU (1) HUP0101961A3 (hu)
IL (1) IL139432A0 (hu)
NO (1) NO20005587L (hu)
NZ (1) NZ507459A (hu)
PL (1) PL343732A1 (hu)
RU (1) RU2229309C2 (hu)
TW (1) TWI225517B (hu)
WO (1) WO1999057263A1 (hu)
ZA (1) ZA200005746B (hu)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6548296B1 (en) 1997-07-23 2003-04-15 Roche Diagnostics Gmbh Methods for identifying human cell lines useful for endogenous gene activation, isolated human lines identified thereby, and uses thereof
US6897066B1 (en) * 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
WO2001042442A2 (en) 1999-12-10 2001-06-14 Cytos Biotechnology Ag Activation of endogenous genes by genomic introduction of a replicon
JP2002017357A (ja) * 2000-07-04 2002-01-22 Calpis Co Ltd アンカーペプチド、融合蛋白質、及び蛋白質の固定化方法
EP2361631A1 (en) 2002-04-25 2011-08-31 Shire Human Genetic Therapies, Inc. Treatment of alpha-galactosidase a deficiency
AU2003296438A1 (en) * 2002-12-24 2004-07-29 North Carolina State University Increasing gamete production with a gene switch
KR100533794B1 (ko) * 2003-10-02 2005-12-07 주식회사 프로젠 인간 난포자극 호르몬을 대량으로 생산하는 방법
ES2389618T3 (es) 2007-04-16 2012-10-29 Momenta Pharmaceuticals, Inc. Productos de glucoproteínas definidas y métodos relacionados
CN101397339B (zh) * 2008-09-24 2012-07-25 上海天伟生物制药有限公司 糖蛋白中去除/灭活病毒的方法
EP2325194A1 (en) 2009-11-24 2011-05-25 Glycotope GmbH Process for the purification of glycoproteins

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639639A (en) 1983-11-02 1997-06-17 Genzyme Corporation Recombinant heterodimeric human fertility hormones, and methods, cells, vectors and DNA for the production thereof
US5272071A (en) 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
US5733761A (en) 1991-11-05 1998-03-31 Transkaryotic Therapies, Inc. Protein production and protein delivery
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery

Also Published As

Publication number Publication date
IL139432A0 (en) 2001-11-25
TWI225517B (en) 2004-12-21
PL343732A1 (en) 2001-09-10
NO20005587D0 (no) 2000-11-06
NZ507459A (en) 2004-01-30
NO20005587L (no) 2001-01-03
US6200778B1 (en) 2001-03-13
HUP0101961A3 (en) 2003-08-28
WO1999057263A9 (en) 2000-03-02
WO1999057263A1 (en) 1999-11-11
AU3881799A (en) 1999-11-23
CZ20003773A3 (cs) 2001-04-11
JP2002513566A (ja) 2002-05-14
US20010034044A1 (en) 2001-10-25
EP1075514A1 (en) 2001-02-14
CA2328506A1 (en) 1999-11-11
KR20010052279A (ko) 2001-06-25
ZA200005746B (en) 2001-05-17
CZ299611B6 (cs) 2008-09-17
AR016262A1 (es) 2001-06-20
CN1308673A (zh) 2001-08-15
AU766620B2 (en) 2003-10-23
RU2229309C2 (ru) 2004-05-27

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