HRP20170875T1 - Modificirane zemljane površine za gensko ciljanje u biljkama - Google Patents
Modificirane zemljane površine za gensko ciljanje u biljkama Download PDFInfo
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- HRP20170875T1 HRP20170875T1 HRP20170875TT HRP20170875T HRP20170875T1 HR P20170875 T1 HRP20170875 T1 HR P20170875T1 HR P20170875T T HRP20170875T T HR P20170875TT HR P20170875 T HRP20170875 T HR P20170875T HR P20170875 T1 HRP20170875 T1 HR P20170875T1
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- nucleic acid
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- acid molecule
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (11)
1. Postupak proizvodnje transgene biljke ili transgenog biljnog tkiva,
naznačen time, da postupak obuhvaća sljedeće:
pripravljanje molekule nukleinske kiseline koja obuhvaća najmanje dva područja sekvence nukleinske kiseline kojima nedostaje homologna sekvenca s genomskom DNK od biljne stanice domaćina, i najmanje jedno mjesto za prepoznavanje ciljne endonukleaze, pri čemu najmanje dva područja sekvence nukleinske kiseline kojima u biti nedostaje homologna sekvenca s genomskom DNK od biljne stanice domaćina, zakriljuju najmanje jedno mjesto prepoznavanja ciljne endonukleaze, gdje najmanje dva područja sekvence nukleinske kiseline kojima nedostaje homologna sekvenca s genomskom DNK od biljne stanice domaćina, ne obuhvaćaju otvoreni okvir za iščitavanje,
dok su pritom najmanje dva područja sekvence nukleinske kiseline kojima nedostaje homologna sekvenca s genomskom DNK od biljne stanice domaćina, odabrana od jedne ili više skupina koje se sastoje od sekvenci SEQ ID NO:1 do SEQ ID NO:14, te 17 ili 18, ili pri čemu najmanje dva područja sekvence nukleinske kiseline kojima nedostaje homologna sekvenca s genomskom DNK od biljne stanice domaćina, zajedno s mjestima ciljne endonukleaze, obuhvaćaju sekvence SEQ ID NO:15, 16 i 19;
transformiranje biljne stanice ili biljnog tkiva koja/e obuhvaća biljnu stanicu s molekulom nukleinske kiseline, gdje je molekula nukleinske kiseline stabilno integrirana u genom biljne stanice; i
generiranje biljnog tkiva ili regeneriranje biljke iz biljne stanice koja je transformirana s molekulom nukleinske kiseline.
2. Postupak prema zahtjevu 1, naznačen time, da molekula nukleinske kiseline nadalje obuhvaća neidentična mjesta restrikcije, a poželjno ta neidentična mjesta restrikcije obuhvaćaju kompatibilne krajeve jednostruke lančane strukture, koji dopuštaju konkatemerizaciju za višestrukih zemljanih podloga koje su podvrgnute genetskom inženjeringu (ELP – engineered landing pad).
3. Postupak prema zahtjevu 1 ili 2, naznačen time, da najmanje dva područja sekvence nukleinske kiseline kojima nedostaje homologna sekvenca s genomskom DNK od biljne stanice domaćina, obuhvaćaju od oko 50 bp do oko 3 kb.
4. Postupak prema bilo kojem od zahtjeva 1 do 3, naznačen time, da mjesto ciljne endonukleaze predstavlja mjesto nukleaze cinkovih prstiju.
5. Postupak prema bilo kojem od zahtjeva 1 do 4, naznačen time, da je molekula nukleinske kiseline stabilno integrirana nasumice u genomu biljne stanice, ili je stabilno integrirana u jedno ili više poznatih ciljnih mjesta u genomu biljne stanice, ili je stabilno integrirana u amplificirano područje kromosoma ili minikromosoma.
6. Postupak prema bilo kojem od zahtjeva 1 do 5, naznačen time, da je molekula nukleinske kiseline zakriljena na svakom kraju pomoću jednog ili više dodatnih mjesta prepoznavanja ciljne endonukleaze.
7. Postupak prema zahtjevu 6, naznačen time, da nadalje obuhvaća uvođenje u biljno tkivo ili u biljku, jedne ili više ciljnih endonukleaza koje prepoznaju jedno ili više dodatnih mjesta prepoznavanja ciljne endonukleaze, pri čemu se molekula nukleinske kiseline odstranjuje iz genoma biljnog tkiva ili biljke.
8. Postupak proizvodnje transgene biljke, naznačen time, da postupak obuhvaća sljedeće:
pripravljanje stanice ili tkiva izolirane(og) iz transgenog biljnog tkiva ili transgene biljke prema zahtjevu 3;
pripravljanje najmanje jedne ciljne endonukleaze ili prve molekule nukleinske kiseline koja obuhvaća sekvencu nukleinske kiseline koja kodira najmanje jednu ciljnu endonukleazu, pri čemu najmanje jedna ciljna endonukleaza prepoznaje najmanje jedno mjesto prepoznavanja ciljne endonukleaze koja je uvedena u genom transgene biljke;
pripravljanje druge molekule nukleinske kiseline koja obuhvaća sekvencu nukleinske kiseline od interesa, i dvije dodatne sekvence nukleinske kiseline koje zakriljuju sekvencu nukleinske kiseline od interesa, pri čemu je svaka od dviju dodatnih sekvenci nukleinske kiseline, homologna s jednim od dva područja sekvence nukleinske kiseline kojima nedostaje homologna sekvenca s genomskom DNK;
uvođenje (i) najmanje jedne ciljne endonukleaze ili prve molekule nukleinske kiseline; i (ii) druge molekule nukleinske kiseline u bilnju stanicu ili biljno tkivo, pri čemu je sekvenca nukleinske kiseline od interesa stabilno integrirana u genom biljne stanice; i
regeneriranje biljke iz biljne stanice ili biljnog tkiva transformirane(og) putem prve i druge molekule nukleinske kiseline.
9. Transgena biljka, naznačena time, da se proizvodi pomoću postupka prema bilo kojem od zahtjeva 1 do 8.
10. Sjeme, naznačeno time, da se proizvodi pomoću transgene biljke prema zahtjevu 9.
11. Postupak prema zahtjevu 7 ili 8, naznačen time, da ciljna endonukleaza predstavlja nukleazu cinkovih prstiju.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29764110P | 2010-01-22 | 2010-01-22 | |
| EP11735271.6A EP2525650B1 (en) | 2010-01-22 | 2011-01-21 | Engineered landing pads for gene targeting in plants |
| PCT/US2011/022145 WO2011091317A2 (en) | 2010-01-22 | 2011-01-21 | Engineered landing pads for gene targeting in plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20170875T1 true HRP20170875T1 (hr) | 2017-08-25 |
Family
ID=44307620
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20170875TT HRP20170875T1 (hr) | 2010-01-22 | 2011-01-21 | Modificirane zemljane površine za gensko ciljanje u biljkama |
Country Status (25)
| Country | Link |
|---|---|
| US (3) | US8802921B2 (hr) |
| EP (2) | EP3047726B1 (hr) |
| JP (2) | JP5939989B2 (hr) |
| KR (1) | KR101948941B1 (hr) |
| CN (2) | CN105671073B (hr) |
| AR (1) | AR079969A1 (hr) |
| AU (2) | AU2011207393B2 (hr) |
| BR (1) | BR112012018235B8 (hr) |
| CA (1) | CA2787594C (hr) |
| CL (1) | CL2012002036A1 (hr) |
| CO (1) | CO6561813A2 (hr) |
| DK (1) | DK2525650T3 (hr) |
| EA (2) | EA031429B1 (hr) |
| GE (1) | GEP201606587B (hr) |
| HR (1) | HRP20170875T1 (hr) |
| HU (1) | HUE033500T2 (hr) |
| IL (2) | IL220869B (hr) |
| MX (2) | MX2012008486A (hr) |
| NZ (1) | NZ600733A (hr) |
| PH (1) | PH12012501490A1 (hr) |
| PT (1) | PT2525650T (hr) |
| SI (1) | SI2525650T1 (hr) |
| UA (1) | UA110472C2 (hr) |
| WO (1) | WO2011091317A2 (hr) |
| ZA (1) | ZA201204578B (hr) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA110472C2 (ru) | 2010-01-22 | 2016-01-12 | Dow Agrosciences Llc | Спосіб отримання клітини трансгенної рослини для спрямованого впливу на ген в рослинах, трансгенна рослина або рослинна тканина, насіння, отримане з трансгенної рослини |
| DK2616488T3 (en) * | 2010-09-15 | 2015-05-26 | Dow Agrosciences Llc | Monoclonal antibodies and methods for detecting enzymes that confer resistance to 2,4-dichlorophenoxyacetic acid |
| AR085533A1 (es) | 2011-03-23 | 2013-10-09 | Pioneer Hi Bred Int | Metodos de produccion de un locus de rasgos transgenicos complejo |
| EP2797407A4 (en) * | 2011-12-30 | 2015-07-08 | Dow Agrosciences Llc | METHOD AND CONSTRUCTION FOR SYNTHETIC BIDIRECTIONAL SCBV PLANT PROMOTER |
| EP2612918A1 (en) | 2012-01-06 | 2013-07-10 | BASF Plant Science Company GmbH | In planta recombination |
| KR102116153B1 (ko) | 2012-05-07 | 2020-05-27 | 상가모 테라퓨틱스, 인코포레이티드 | 도입유전자의 뉴클레아제-매개 표적화된 통합을 위한 방법 및 조성물 |
| UA119135C2 (uk) | 2012-09-07 | 2019-05-10 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб отримання трансгенної рослини |
| UA118090C2 (uk) | 2012-09-07 | 2018-11-26 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив |
| KR101449141B1 (ko) | 2012-11-07 | 2014-10-08 | 현대자동차주식회사 | 차량의 폐열 회수 시스템을 이용한 터보장치 |
| US9493844B2 (en) | 2012-12-13 | 2016-11-15 | Dow Agrosciences Llc | DNA detection methods for site specific nuclease activity |
| EP3041344A4 (en) | 2013-09-04 | 2017-04-19 | Dow AgroSciences LLC | Rapid targeting analysis in crops for determining donor insertion |
| CN105683357B (zh) * | 2013-11-04 | 2020-10-30 | 美国陶氏益农公司 | 用于基因打靶的通用供体系统 |
| UY35812A (es) | 2013-11-04 | 2015-05-29 | Dow Agrosciences Llc | ?loci de maíz óptimos?. |
| CA2928855A1 (en) | 2013-11-04 | 2015-05-07 | Dow Agrosciences Llc | Optimal maize loci |
| WO2015066643A1 (en) | 2013-11-04 | 2015-05-07 | Dow Agrosciences Llc | Optimal soybean loci |
| RU2017112324A (ru) | 2014-09-12 | 2018-10-15 | Пайонир Хай-Бред Интернэшнл, Инк. | Создание сайтов сайт-специфической интеграции для сложных локусов признаков в кукурузе и сое, а также способы применения |
| US10616219B2 (en) * | 2014-12-11 | 2020-04-07 | FlowJo, LLC | Single cell data management and analysis systems and methods |
| CA2969151A1 (en) | 2014-12-23 | 2016-06-30 | Syngenta Participations Ag | Methods and compositions for identifying and enriching for cells comprising site specific genomic modifications |
| WO2017201311A2 (en) | 2016-05-18 | 2017-11-23 | Amyris, Inc. | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
| US11634721B2 (en) * | 2016-12-14 | 2023-04-25 | Dow Agrosciences Llc | Reconstruction of site specific nuclease binding sites |
| US12300357B2 (en) | 2016-12-14 | 2025-05-13 | FlowJo, LLC | Applied computer technology for management, synthesis, visualization, and exploration of parameters in large multi-parameter data sets |
| US10828330B2 (en) * | 2017-02-22 | 2020-11-10 | IO Bioscience, Inc. | Nucleic acid constructs comprising gene editing multi-sites and uses thereof |
| ES2980676T3 (es) | 2017-05-25 | 2024-10-02 | Flowjo Llc | Visualización, análisis comparativo y detección de diferencias automatizada para grandes conjuntos de datos multiparamétricos |
| US11214811B1 (en) | 2020-07-31 | 2022-01-04 | Inari Agriculture Technology, Inc. | INIR6 transgenic maize |
| US11369073B2 (en) | 2020-07-31 | 2022-06-28 | Inari Agriculture Technology, Inc. | INIR12 transgenic maize |
| US20240011043A1 (en) | 2020-07-31 | 2024-01-11 | Inari Agriculture Technology, Inc. | Generation of plants with improved transgenic loci by genome editing |
| US11242534B1 (en) | 2020-07-31 | 2022-02-08 | Inari Agriculture Technology, Inc. | INHT31 transgenic soybean |
| US11326177B2 (en) | 2020-07-31 | 2022-05-10 | Inari Agriculture Technology, Inc. | INIR12 transgenic maize |
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| US5641876A (en) | 1990-01-05 | 1997-06-24 | Cornell Research Foundation, Inc. | Rice actin gene and promoter |
| DE69334225D1 (de) | 1992-07-07 | 2008-07-31 | Japan Tobacco Inc | Verfahren zur transformation einer monokotyledon pflanze |
| JP3329819B2 (ja) | 1993-09-03 | 2002-09-30 | 日本たばこ産業株式会社 | 未熟胚の胚盤を用いた単子葉植物の形質転換方法 |
| DK0991764T3 (da) | 1997-06-12 | 2006-11-13 | Dow Agrosciences Llc | Regulatoriske sekvenser for transgene planter |
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| CA2428972A1 (en) * | 2003-05-30 | 2004-11-30 | Wyeth | Conditional knockout method for gene trapping and gene targeting using an inducible gene silencer |
| CA2897475C (en) | 2004-04-30 | 2018-07-10 | Dow Agrosciences Llc | Novel herbicide resistance genes |
| KR20080033455A (ko) | 2005-07-26 | 2008-04-16 | 상가모 바이오사이언스 인코포레이티드 | 외래 핵산 서열의 표적화된 통합 및 발현 |
| US8278505B2 (en) | 2007-05-09 | 2012-10-02 | Dow Agrosciences, Llc. | Herbicide resistance genes for resistance to aryloxyalkanoate herbicides |
| AU2010339481B2 (en) * | 2009-12-30 | 2016-02-04 | Pioneer Hi-Bred International, Inc. | Methods and compositions for targeted polynucleotide modification |
| UA110472C2 (ru) | 2010-01-22 | 2016-01-12 | Dow Agrosciences Llc | Спосіб отримання клітини трансгенної рослини для спрямованого впливу на ген в рослинах, трансгенна рослина або рослинна тканина, насіння, отримане з трансгенної рослини |
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2011
- 2011-01-21 UA UAA201210025A patent/UA110472C2/ru unknown
- 2011-01-21 EP EP15197514.1A patent/EP3047726B1/en not_active Not-in-force
- 2011-01-21 EA EA201690178A patent/EA031429B1/ru not_active IP Right Cessation
- 2011-01-21 NZ NZ600733A patent/NZ600733A/en not_active IP Right Cessation
- 2011-01-21 GE GEAP201112842A patent/GEP201606587B/en unknown
- 2011-01-21 PH PH1/2012/501490A patent/PH12012501490A1/en unknown
- 2011-01-21 KR KR1020127021853A patent/KR101948941B1/ko active Active
- 2011-01-21 MX MX2012008486A patent/MX2012008486A/es active IP Right Grant
- 2011-01-21 CN CN201610031616.4A patent/CN105671073B/zh not_active Expired - Fee Related
- 2011-01-21 CN CN201180015548.8A patent/CN102821598B/zh not_active Expired - Fee Related
- 2011-01-21 EA EA201290676A patent/EA027914B1/ru not_active IP Right Cessation
- 2011-01-21 SI SI201131214A patent/SI2525650T1/sl unknown
- 2011-01-21 BR BR112012018235A patent/BR112012018235B8/pt not_active IP Right Cessation
- 2011-01-21 CA CA2787594A patent/CA2787594C/en active Active
- 2011-01-21 EP EP11735271.6A patent/EP2525650B1/en active Active
- 2011-01-21 AU AU2011207393A patent/AU2011207393B2/en not_active Ceased
- 2011-01-21 HU HUE11735271A patent/HUE033500T2/en unknown
- 2011-01-21 MX MX2015005655A patent/MX351417B/es unknown
- 2011-01-21 DK DK11735271.6T patent/DK2525650T3/en active
- 2011-01-21 PT PT117352716T patent/PT2525650T/pt unknown
- 2011-01-21 JP JP2012550176A patent/JP5939989B2/ja not_active Expired - Fee Related
- 2011-01-21 HR HRP20170875TT patent/HRP20170875T1/hr unknown
- 2011-01-21 WO PCT/US2011/022145 patent/WO2011091317A2/en active Application Filing
- 2011-01-21 US US13/011,735 patent/US8802921B2/en not_active Expired - Fee Related
- 2011-01-24 AR ARP110100223A patent/AR079969A1/es active IP Right Grant
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2012
- 2012-06-20 ZA ZA2012/04578A patent/ZA201204578B/en unknown
- 2012-07-10 IL IL220869A patent/IL220869B/en active IP Right Grant
- 2012-07-18 CO CO12121225A patent/CO6561813A2/es active IP Right Grant
- 2012-07-20 CL CL2012002036A patent/CL2012002036A1/es unknown
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2014
- 2014-08-12 US US14/458,086 patent/US10160975B2/en not_active Expired - Fee Related
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2015
- 2015-08-07 AU AU2015210438A patent/AU2015210438B2/en not_active Ceased
- 2015-08-26 IL IL240852A patent/IL240852B/en active IP Right Grant
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2016
- 2016-03-04 JP JP2016041973A patent/JP6105112B2/ja not_active Expired - Fee Related
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2018
- 2018-12-04 US US16/209,028 patent/US11008578B2/en not_active Expired - Fee Related
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