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GB972400A - Process for preparing kallikrein - Google Patents

Process for preparing kallikrein

Info

Publication number
GB972400A
GB972400A GB1853162A GB1853162A GB972400A GB 972400 A GB972400 A GB 972400A GB 1853162 A GB1853162 A GB 1853162A GB 1853162 A GB1853162 A GB 1853162A GB 972400 A GB972400 A GB 972400A
Authority
GB
United Kingdom
Prior art keywords
kallikrein
preparing
subjecting
dissolving
water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1853162A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Original Assignee
Bayer AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG filed Critical Bayer AG
Publication of GB972400A publication Critical patent/GB972400A/en
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/6445Kallikreins (3.4.21.34; 3.4.21.35)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Kallikrein is prepared by the fractional precipitation of pigs' urine with acetone, collecting the kallikrein-containing precipitate and dissolving it in water, subjecting the solution obtained to a further acetone precipitation, dissolving the precipitate obtained in water and subjecting the aqueous solution obtained to chromatography on a polydextrane column to obtain a colourless, aqueous solution of kallikrein containing less than 10 m g of protein per KU.
GB1853162A 1961-05-27 1962-05-14 Process for preparing kallikrein Expired GB972400A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEF0034027 1961-05-27

Publications (1)

Publication Number Publication Date
GB972400A true GB972400A (en) 1964-10-14

Family

ID=7095376

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1853162A Expired GB972400A (en) 1961-05-27 1962-05-14 Process for preparing kallikrein

Country Status (1)

Country Link
GB (1) GB972400A (en)

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