[go: up one dir, main page]

GB2318792B - Unactivated oocytes as cytoplast recipients for nuclear transfer - Google Patents

Unactivated oocytes as cytoplast recipients for nuclear transfer

Info

Publication number
GB2318792B
GB2318792B GB9802916A GB9802916A GB2318792B GB 2318792 B GB2318792 B GB 2318792B GB 9802916 A GB9802916 A GB 9802916A GB 9802916 A GB9802916 A GB 9802916A GB 2318792 B GB2318792 B GB 2318792B
Authority
GB
United Kingdom
Prior art keywords
time
embryo
transfer
oocyte
nucleus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB9802916A
Other versions
GB9802916D0 (en
GB2318792A (en
Inventor
Keith Henry Stockman Campbell
Ian Wilmut
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Minister of Agriculture Fisheries and Food UK
Biotechnology and Biological Sciences Research Council
Roslin Institute
Original Assignee
Minister of Agriculture Fisheries and Food UK
Biotechnology and Biological Sciences Research Council
Roslin Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9517779.6A external-priority patent/GB9517779D0/en
Application filed by Minister of Agriculture Fisheries and Food UK, Biotechnology and Biological Sciences Research Council, Roslin Institute filed Critical Minister of Agriculture Fisheries and Food UK
Priority to GB9926730A priority Critical patent/GB2340493B/en
Priority to GB9802916A priority patent/GB2318792B/en
Publication of GB9802916D0 publication Critical patent/GB9802916D0/en
Publication of GB2318792A publication Critical patent/GB2318792A/en
Application granted granted Critical
Publication of GB2318792B publication Critical patent/GB2318792B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the G0 or G1 phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor much as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.
GB9802916A 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer Expired - Fee Related GB2318792B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB9926730A GB2340493B (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer
GB9802916A GB2318792B (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9517779.6A GB9517779D0 (en) 1995-08-31 1995-08-31 Biological manipulation
PCT/GB1996/002098 WO1997007668A1 (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer
GB9802916A GB2318792B (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer

Publications (3)

Publication Number Publication Date
GB9802916D0 GB9802916D0 (en) 1998-04-08
GB2318792A GB2318792A (en) 1998-05-06
GB2318792B true GB2318792B (en) 2000-06-21

Family

ID=26307655

Family Applications (2)

Application Number Title Priority Date Filing Date
GB9802916A Expired - Fee Related GB2318792B (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer
GB9926730A Expired - Fee Related GB2340493B (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer

Family Applications After (1)

Application Number Title Priority Date Filing Date
GB9926730A Expired - Fee Related GB2340493B (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer

Country Status (1)

Country Link
GB (2) GB2318792B (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2265909A (en) * 1992-04-01 1993-10-13 Inst Of Animal Physiology And Nuclear transplantation
WO1994006422A1 (en) * 1992-09-22 1994-03-31 The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services Use of taxol for treating lymphomas and breast cancer
WO1995003795A1 (en) * 1993-07-29 1995-02-09 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Method of treating atherosclerosis or restenosis using microtubule stabilizing agent

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9308271D0 (en) * 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2265909A (en) * 1992-04-01 1993-10-13 Inst Of Animal Physiology And Nuclear transplantation
WO1994006422A1 (en) * 1992-09-22 1994-03-31 The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services Use of taxol for treating lymphomas and breast cancer
WO1995003795A1 (en) * 1993-07-29 1995-02-09 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Method of treating atherosclerosis or restenosis using microtubule stabilizing agent

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Biology of Reproduction 1990,43(5),877-884 *
Biology of Reproduction 1993,49(5),933-942 *
J.Cell Science 1984,69,19-34 *
Molecular Reproduction and Development 1994,39(2),147-152 *
Nature 1986,320(6),63-65 *
The Wistar Institute Symposium Monograph 1969,1,19-33 *
Theriogenology 1996,45(1),287 *

Also Published As

Publication number Publication date
GB9802916D0 (en) 1998-04-08
GB2318792A (en) 1998-05-06
GB2340493A (en) 2000-02-23
GB2340493B (en) 2000-06-21
GB9926730D0 (en) 2000-01-12

Similar Documents

Publication Publication Date Title
SG75919A1 (en) Unactivated oocytes as cytoplast recipients for nuclear transfer
GB2318578A (en) Quiescent cell populations for nuclear transfer
ES2246227T8 (en) Nucleic acid molecules encoding alternansacarase
AU2002361327A1 (en) Exogenous protein expression system in an avian system
EP0938550A4 (en) CULTURE OF A CELL LINE FROM THE INNER CELL SIZE OF HIP ANIMAL EMBRYONS.
CY1105006T1 (en) METHOD OF PRODUCING AND RAISING CHICKS, AS WELL AS HATCHING ARRANGEMENT AND CHICK FARM FOR CARRYING OUT THE METHOD
DK0485044T3 (en) Plasmids for the production of transgenic plants modified for growth form and yield
EP1198169A4 (en) A process of cell reprogramming through production of a heterokaryon
NO882469D0 (en) PROCEDURE FOR PRODUCING PROTEINS USING USE-INJURABLE EXPRESSION SYSTEMS IN GENETICALLY MODIFIED EUCARYOTIC HOST CELLS FORMED IN VIVO.
NZ336493A (en) A method of oocyte enucleation and production of reconstituted embryos
GB2318792B (en) Unactivated oocytes as cytoplast recipients for nuclear transfer
GB2369828A (en) Nuclear transfer with selected donor cells
Miki et al. Cytoplasmic asters are required for progression past the first cell cycle in cloned mouse embryos
WO2000025578A3 (en) Methods for cloning animals
GB2331751B (en) Quiescent cell populations for nuclear transfer
DE60041771D1 (en) METHOD FOR TRANSFERRING SPERMATONIAL GERM CELLS ON CROPS
DE59814285D1 (en) EFFICIENT CORE TRANSFER WITH PRIMORDIAL GERM CELLS
RU98105617A (en) INACTIVATED OOCYTES AS RECIPIENT CYTOPLASTS FOR NUCLEAR TRANSFER
Latham et al. Modelling Habituation of Introduced Predators to Unrewarding Bird Odors for Conservation of Ground-Nesting Shorebirds
김선균 et al. In vitro Development of Somatic Cell Nuclear Transferred Bovine Embryos Following Activation Timing in MII Enucleated Oocytes Cryopreservation
BG49622A1 (en) Method for obtaining male- sterile plants at the sunflower

Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20130830