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NZ336493A - A method of oocyte enucleation and production of reconstituted embryos - Google Patents

A method of oocyte enucleation and production of reconstituted embryos

Info

Publication number
NZ336493A
NZ336493A NZ336493A NZ33649397A NZ336493A NZ 336493 A NZ336493 A NZ 336493A NZ 336493 A NZ336493 A NZ 336493A NZ 33649397 A NZ33649397 A NZ 33649397A NZ 336493 A NZ336493 A NZ 336493A
Authority
NZ
New Zealand
Prior art keywords
production
oocyte
cytoplasm
polar body
embryos
Prior art date
Application number
NZ336493A
Inventor
Teija Peura
Original Assignee
Univ Monash
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AUPO4432A external-priority patent/AUPO443297A0/en
Priority claimed from AUPO4431A external-priority patent/AUPO443197A0/en
Application filed by Univ Monash filed Critical Univ Monash
Publication of NZ336493A publication Critical patent/NZ336493A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0609—Oocytes, oogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/59—Lectins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

An oocyte having a polar body, metaphase plate and cytoplasm is subjected to a compound such as a phytohemagglutinin (PHA) aggregate solution, which causes attachment of the polar body to the oocyte, and then enucleated by separating the polar body and a portion of cytoplasm containing the metaphase plate from remaining cytoplasm. The techniques are useful in nuclear transplantation for the production of nuclear transfer (particularly bovine) embryos and multiple genetically similar offspring.
NZ336493A 1997-01-02 1997-12-22 A method of oocyte enucleation and production of reconstituted embryos NZ336493A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPO4432A AUPO443297A0 (en) 1997-01-02 1997-01-02 Method of oocyte enucleation
AUPO4431A AUPO443197A0 (en) 1997-01-02 1997-01-02 Production of reconstituted embryos
PCT/AU1997/000868 WO1998029532A1 (en) 1997-01-02 1997-12-22 A method of oocyte enucleation and production of reconstituted embryos

Publications (1)

Publication Number Publication Date
NZ336493A true NZ336493A (en) 2001-01-26

Family

ID=25645342

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ336493A NZ336493A (en) 1997-01-02 1997-12-22 A method of oocyte enucleation and production of reconstituted embryos

Country Status (2)

Country Link
NZ (1) NZ336493A (en)
WO (1) WO1998029532A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143564A (en) * 1998-07-07 2000-11-07 University Of Hawaii Use of the polar body chromosomes for the production of embryos and normal offspring
EP1818397A1 (en) * 1998-11-02 2007-08-15 Trustees Of Tufts College Methods for cloning animals
WO2000025578A2 (en) * 1999-04-26 2000-05-11 Trustees Of Tufts College Methods for cloning animals
US6781030B1 (en) 1998-11-02 2004-08-24 Trustee Of Tufts College, Ballou Hall Methods for cloning mammals using telophase oocytes
NZ524523A (en) 2000-08-03 2006-02-24 Therapeutic Human Polyclonals Production of humanized antibodies in transgenic animals
AUPR451401A0 (en) * 2001-04-20 2001-05-24 Monash University A method of nuclear transfer
US20030113910A1 (en) * 2001-12-18 2003-06-19 Mike Levanduski Pluripotent stem cells derived without the use of embryos or fetal tissue
US7612250B2 (en) 2002-07-29 2009-11-03 Trustees Of Tufts College Nuclear transfer embryo formation method
KR101383825B1 (en) 2005-09-08 2014-04-10 오르후스 우니베르시테트 Cell nuclear transfer
PE20081216A1 (en) 2006-09-01 2008-09-04 Therapeutic Human Polyclonals Inc ENHANCED EXPRESSION OF HUMAN OR HUMANIZED IMMUNOGLOBULIN IN NON-HUMAN TRANSGENIC ANIMALS
CN107794281B (en) * 2017-11-10 2021-06-01 南开大学 Augmented reality-based fixed-point de-kerneling method
CN108823067B (en) * 2018-07-23 2023-08-11 中国农业科学院北京畜牧兽医研究所 Cell enucleation micromanipulation needle and cell enucleation method
CN114107180B (en) * 2021-10-21 2024-03-08 湖北省农业科学院畜牧兽医研究所 Zona pellucida-free somatic clone 1-cell stage embryo polymerization and in vitro culture method

Also Published As

Publication number Publication date
WO1998029532A1 (en) 1998-07-09

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