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GB2224118A - Air sampler - Google Patents

Air sampler Download PDF

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Publication number
GB2224118A
GB2224118A GB8819423A GB8819423A GB2224118A GB 2224118 A GB2224118 A GB 2224118A GB 8819423 A GB8819423 A GB 8819423A GB 8819423 A GB8819423 A GB 8819423A GB 2224118 A GB2224118 A GB 2224118A
Authority
GB
United Kingdom
Prior art keywords
petri dish
air
cavity
air sampler
orifice plate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB8819423A
Other versions
GB8819423D0 (en
GB2224118B (en
Inventor
Geoffrey Malcolm Wili
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BURKARD MANUFACTURING Co
Original Assignee
BURKARD MANUFACTURING Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BURKARD MANUFACTURING Co filed Critical BURKARD MANUFACTURING Co
Priority to GB8819423A priority Critical patent/GB2224118B/en
Publication of GB8819423D0 publication Critical patent/GB8819423D0/en
Publication of GB2224118A publication Critical patent/GB2224118A/en
Application granted granted Critical
Publication of GB2224118B publication Critical patent/GB2224118B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/2202—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/2202—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
    • G01N2001/222—Other features
    • G01N2001/2223—Other features aerosol sampling devices

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

An air sampler e.g. for determining the bacteria content in air, has a housing (1) containing e.g. a turbine disc (9, 10) driven by an electric motor (5) powered by a battery (6) which is rechargeable via a charge socket. Above the turbine is a cavity (24) above which is supported a Petri Dish (16) which supports an orifice plate (17). The turbine draws air through the orifices in the orifice plate (17) in the form of small jets impinging onto the surface of a medium in the Petri Dish. This air is then drawn radially outwardly and upwardly over the rim of the Petri Dish and down to the turbine to be ejected through orifices (13). <IMAGE>

Description

AIR SAMPLER The present invention relates to air samplers for the collection of viable airborne particles.
According to the present invention, there is provided a sampler comprising a base having a cavity therein and an outlet and a lower part thereof through which air may be continuously extracted from the cavity, means in the cavity for supporting a Petri Dish in spaced relation to the bottom of the cavity and a orifice plate adapted to extend over the whole of the Petri Dish and being formed with an array of orifices over substantially the whole effective area of the Petri Dish and means for supporting the orifice plate at a predetermined height in relation to the Petri Dish while defining a substantially complete annular air passage between the orifice plate and the rim of the Petri Dish.
Advantageously, the orifice plate has three spacing lugs positioned to engage directly on the upper edge of the Petri Dish rim.
An embodiment of the invention will now be described by way of example with reference to the accompanying drawings in which: Figure 1 is a front view, partly in axial section, of an air sampler; and Figure 2 is a top view of the air sampler shown in Figure 1 with the cover removed and parts broken away.
The air sampler shown in the drawings has an integral motor-driven fan contained within an inverted, cup-shaped housing 1 the lower end of which fits over a cylindrical wall 2 upstanding from a circular base member 3 which is supported on a suitable surface by three feet 4 formed by rubber buffers.
Secured to the base member 3 is an electric motor 5, a rechargable battery 6 for firing the motor and an on-off switch 7 for connecting the motor 5 to, or disconnecting it from, the battery 6 which in turn is connected to a charging socket (not shown) to enable it to be recharged by plugging in a connection from an outside source.
The motor 5 has its shaft 8 vertical and carrying at its upper end a turbine formed by two spaced horizontal disks 9 and 10 separated by radially extending rods 11. In the centre of the upper disk 9 is an air inlet formed by a raised mouth 12. The housing 1 is formed with a ring of air outlet apertures 13.
Secured to the wall of the housing 1 is a base member 14 of a Petri Dish holder assembly. Secured in the base 14 are three flanged pegs 15 which locate horizontally and also support by means of their flanges a standard Petri Dish 16 which in use contains a body of culture medium for growing colonies of viable particles such as bacteria under investigation.
Supported on the rim of the Petri Dish 16 is a perforated disk 17 having an outer rim 18 of inverted "U" cross-section the outer part of which fits within an upper wall 19 of the base 14. Secured to the rim 18 at equally angularly spaced positions are three lugs 21 which rest on the upper edge of the rim of the Petri Dish 16 and thus define an air passage 22 formed by three arcuate sections between the lugs 21.
Beneath the Petri Dish, the base 14 defines a flat conical space 24 leading down to an air outlet 25 which registers with the mouth 12 of the turbine. A cover 30 having an orifice is slidable in grooves in the air outlet 25 to close the outlet 25 selectively.
In operation, with a fresh Petri Dish 17 positioned on the pegs 15, and the perforated disk 17 placed in position over it, an upper cover 26 may be placed over the side wall 19 of the base 14. The upper cover 26 has a central opening 27 which can be closed by a suitable closure (not shown) when the apparatus is not in use.
To sample the air, the cover 30 is slid to its closed position and the motor 5 is switched on by means of a switch 7. When the motor reaches normal running speed, the cover is opened and the turbine then draws air through the apparatus in such a way that each perforation 28 delivers a small jet of air onto the surface of the medium in the Petri Dish. This air is drawn radially outwardly and upwardly over the rim of the Petri Dish, around the underside of the rim 18 through the passages 22 and down through the gap 29 between the Petri Dish and the base 14 to enter the space 24 from which the air is drawn through the outlet 25 into the mouth 12 of the turbine, thence to be expelled through the outlet apertures 13.
The apparatus is run for a predetermined time after which the Petri Dish can be removed to enable the bacteria collected to be incubated for subsequent analysis.

Claims (7)

Claims
1. An air sampler comprising a base having a cavity therein and an outlet in a lower part of the cavity through which air may be continuously extracted from the cavity, means in the cavity for supporting a Petri Dish in spaced relation to the bottom of the cavity, an orifice plate adapted to extend over the whole of the Petri Dish and being formed with an array of orifices over sUbstantially the whole effective area of the Petri Dish and means for supporting the orifice plate at a predetermined height in relation to the Petri Dish while defining a substantially complete annular air passage between the orifice plate and the rim of the Petri Dish.
2. An air sampler according to claim 1, including closure means for selectively closing the outlet from the cavity.
3. An air sampler according to claim 1 or 2, wherein the orifice plate has three spacing lugs positioned to engage directly on the upper edge of the Petri Dish rim.
4. An air sampler according to any of claims 1 to 3, and including an upper cover detachably supported on the base and extending upwards to a central opening.
5. An air sampler according to any of claims 1 to 4, wfierein a housing below the cavity contains a turbine for drawing air from the cavity, an electric motor for rotating the turbine and a battery for powering the motor.
6. An air sampler according to claim 5, wherein the battery is rechargeable and is connected to a charging socket.
7. An air sampler substantially as described with reference to the drawings.
GB8819423A 1988-08-16 1988-08-16 Air sampler Expired - Fee Related GB2224118B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB8819423A GB2224118B (en) 1988-08-16 1988-08-16 Air sampler

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8819423A GB2224118B (en) 1988-08-16 1988-08-16 Air sampler

Publications (3)

Publication Number Publication Date
GB8819423D0 GB8819423D0 (en) 1988-09-21
GB2224118A true GB2224118A (en) 1990-04-25
GB2224118B GB2224118B (en) 1991-10-23

Family

ID=10642185

Family Applications (1)

Application Number Title Priority Date Filing Date
GB8819423A Expired - Fee Related GB2224118B (en) 1988-08-16 1988-08-16 Air sampler

Country Status (1)

Country Link
GB (1) GB2224118B (en)

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2274513A (en) * 1993-01-25 1994-07-27 Winton Eurotech Limited Monitoring air quality
WO1996031594A1 (en) * 1995-04-06 1996-10-10 Ultra Propre Nutrition Industrie Recherche (U.N.I.R.) Microbiological pressurised gas control device
EP0964240A1 (en) * 1998-06-10 1999-12-15 Millipore S.A. Sampling apparatus for the microbiological analysis of air
EP0964241A1 (en) * 1998-06-10 1999-12-15 Millipore S.A. Sampling method and sampling apparatus for microbiological analysis of air
US6040153A (en) * 1998-04-24 2000-03-21 Millipore Corporation Cartridge as well as air analysis method and apparatus using it
US6043049A (en) * 1998-04-24 2000-03-28 Millipore S.A. Method for detecting micro-organisms and cartridge suitable for implementing it
EP1008646A1 (en) * 1998-11-13 2000-06-14 van den Wildenberg, Pierre Culture medium container with integrated air suction and circulation geometry for aerial germs
EP1059520A2 (en) * 1999-06-09 2000-12-13 Air Dispersions Ltd. Gas sampling assemblies
GB2353355A (en) * 1999-08-18 2001-02-21 Geoffrey Malcolm Wili Air sampler for particulate collection
EP1126021A1 (en) * 1998-10-26 2001-08-22 Midori Anzen Co., Ltd. Portable air-borne bacteria sampler
FR2820432A1 (en) * 2001-02-06 2002-08-09 Thierry Roger Garcia Airborne micro-organism sampler grille has safety projections to prevent it being fitted before Petri dish cover is removed
EP1233056A1 (en) * 1999-11-25 2002-08-21 Midori Anzen Co., Ltd. Portable air-borne bacteria sampler
WO2003004996A2 (en) * 2001-07-03 2003-01-16 Biochem Tech, Llc Air sampler for pathogens and psychrometrics
WO2003081212A2 (en) * 2002-03-16 2003-10-02 Pathogenus, Inc. Adjustable air sampler with psychrometrics for viable and non-viable aerosols
WO2004003516A1 (en) * 2002-06-28 2004-01-08 Biophage Inc. Method, device and system for detecting the presence of microorganisms
FR2876115A1 (en) * 2004-10-06 2006-04-07 Acanthe Sarl METHOD, DEVICE AND SYSTEM FOR AIR COLLECTION FOR MICROBIOLOGICAL ANALYSIS
WO2022232510A1 (en) * 2021-04-30 2022-11-03 Root Applied Sciences Inc. Device for collecting material from air

Cited By (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2274513A (en) * 1993-01-25 1994-07-27 Winton Eurotech Limited Monitoring air quality
WO1996031594A1 (en) * 1995-04-06 1996-10-10 Ultra Propre Nutrition Industrie Recherche (U.N.I.R.) Microbiological pressurised gas control device
FR2732692A1 (en) * 1995-04-06 1996-10-11 Unir DEVICE FOR MICROBIOLOGICAL CONTROL OF A GAS UNDER PRESSURE
US6294375B1 (en) 1995-04-06 2001-09-25 Ultra Propre Nutrition Industrie Recherche (U.N.I.R.) Microbiological pressurised gas control device
US6040153A (en) * 1998-04-24 2000-03-21 Millipore Corporation Cartridge as well as air analysis method and apparatus using it
US6043049A (en) * 1998-04-24 2000-03-28 Millipore S.A. Method for detecting micro-organisms and cartridge suitable for implementing it
EP0964241A1 (en) * 1998-06-10 1999-12-15 Millipore S.A. Sampling method and sampling apparatus for microbiological analysis of air
FR2779823A1 (en) * 1998-06-10 1999-12-17 Millipore Sa SAMPLING APPARATUS FOR MICROBIOLOGICAL AIR ANALYSIS
FR2779822A1 (en) * 1998-06-10 1999-12-17 Millipore Sa SAMPLING METHOD AND APPARATUS FOR MICROBIOLOGICAL AIR ANALYSIS
US6094997A (en) * 1998-06-10 2000-08-01 Millipore S.A. Sampling apparatus for the microbiological analysis of air
US6240768B1 (en) 1998-06-10 2001-06-05 Millipore S.A. Sampling method and sampling apparatus for the microbiological analysis of air
EP0964240A1 (en) * 1998-06-10 1999-12-15 Millipore S.A. Sampling apparatus for the microbiological analysis of air
EP1126021A4 (en) * 1998-10-26 2004-04-14 Midori Anzen Co Ltd Portable air-borne bacteria sampler
EP1126021A1 (en) * 1998-10-26 2001-08-22 Midori Anzen Co., Ltd. Portable air-borne bacteria sampler
EP1008646A1 (en) * 1998-11-13 2000-06-14 van den Wildenberg, Pierre Culture medium container with integrated air suction and circulation geometry for aerial germs
US6342388B1 (en) 1998-11-13 2002-01-29 Pierre Van Den Wildenberg Culture medium container, with integrated geometry for air suction and air conduction, for the purpose of air bacteria analysis
EP1059520A3 (en) * 1999-06-09 2002-01-16 Air Dispersions Ltd. Gas sampling assemblies
US6463815B1 (en) 1999-06-09 2002-10-15 Air Dispersions Ltd Gas sampling assemblies
EP1059520A2 (en) * 1999-06-09 2000-12-13 Air Dispersions Ltd. Gas sampling assemblies
GB2353355B (en) * 1999-08-18 2003-07-09 Geoffrey Malcolm Wili Air sampler
GB2353355A (en) * 1999-08-18 2001-02-21 Geoffrey Malcolm Wili Air sampler for particulate collection
EP1233056A1 (en) * 1999-11-25 2002-08-21 Midori Anzen Co., Ltd. Portable air-borne bacteria sampler
EP1233056A4 (en) * 1999-11-25 2006-05-17 Midori Anzen Co Ltd Portable air-borne bacteria sampler
FR2820432A1 (en) * 2001-02-06 2002-08-09 Thierry Roger Garcia Airborne micro-organism sampler grille has safety projections to prevent it being fitted before Petri dish cover is removed
WO2003004996A2 (en) * 2001-07-03 2003-01-16 Biochem Tech, Llc Air sampler for pathogens and psychrometrics
WO2003004996A3 (en) * 2001-07-03 2003-06-19 Biochem Tech Llc Air sampler for pathogens and psychrometrics
WO2003081212A2 (en) * 2002-03-16 2003-10-02 Pathogenus, Inc. Adjustable air sampler with psychrometrics for viable and non-viable aerosols
WO2003081212A3 (en) * 2002-03-16 2004-03-25 Pathogenus Inc Adjustable air sampler with psychrometrics for viable and non-viable aerosols
WO2004003516A1 (en) * 2002-06-28 2004-01-08 Biophage Inc. Method, device and system for detecting the presence of microorganisms
FR2876115A1 (en) * 2004-10-06 2006-04-07 Acanthe Sarl METHOD, DEVICE AND SYSTEM FOR AIR COLLECTION FOR MICROBIOLOGICAL ANALYSIS
WO2006037897A1 (en) * 2004-10-06 2006-04-13 Acanthe Air sampling method, device and system for microbiological analysis
WO2022232510A1 (en) * 2021-04-30 2022-11-03 Root Applied Sciences Inc. Device for collecting material from air

Also Published As

Publication number Publication date
GB8819423D0 (en) 1988-09-21
GB2224118B (en) 1991-10-23

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20050816