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GB1501563A - Biosynthesis of microbial insecticides - Google Patents

Biosynthesis of microbial insecticides

Info

Publication number
GB1501563A
GB1501563A GB20740/76A GB2074076A GB1501563A GB 1501563 A GB1501563 A GB 1501563A GB 20740/76 A GB20740/76 A GB 20740/76A GB 2074076 A GB2074076 A GB 2074076A GB 1501563 A GB1501563 A GB 1501563A
Authority
GB
United Kingdom
Prior art keywords
cultivation
aeration
product
bacillus
microbial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB20740/76A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CRC Compagnie di Ricerca Chimica SpA
Original Assignee
CRC Compagnie di Ricerca Chimica SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CRC Compagnie di Ricerca Chimica SpA filed Critical CRC Compagnie di Ricerca Chimica SpA
Priority to GB20740/76A priority Critical patent/GB1501563A/en
Publication of GB1501563A publication Critical patent/GB1501563A/en
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50Isolated enzymes; Isolated proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07Bacillus
    • C12R2001/075Bacillus thuringiensis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Sustainable Development (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

1501563 Microbial insecticides CRC COMPAGNIA DI RICERCA CHIMICA SA 19 May 1976 20740/76 Heading A5E [Also in Division C6] In cultivating Bacillus thuringiensis or a related Bacillus in submerged culture with aeration to produce a microbial insecticide product containing spores of the Bacillus and crystalline endotoxin, early lysis of the cells otherwise caused by the aeration during the submerged cultivation before the end of spore formation is prevented by either or both of the following adjustments: (1) discontinuing aeration and temporarily carrying out the cultivation under anaerobic conditions e.g. for 3 to 6 hours from the twelth hour of cultivation, and (2) maintaining the pH of the culture medium in the range 6.3 to 7.0. The spore formation, with the liberation of endotoxin crystals is complete after 35 to 40 hours cultivation. The microbial insecticide product is freed from toxic, water-soluble exotoxin by dialysis and is then dried by conventional procedures (specified). Bentonite clay may be introduced into the product before the drying. The dehydrated product may be comminuted, if necessary.
GB20740/76A 1976-05-19 1976-05-19 Biosynthesis of microbial insecticides Expired GB1501563A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB20740/76A GB1501563A (en) 1976-05-19 1976-05-19 Biosynthesis of microbial insecticides

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB20740/76A GB1501563A (en) 1976-05-19 1976-05-19 Biosynthesis of microbial insecticides

Publications (1)

Publication Number Publication Date
GB1501563A true GB1501563A (en) 1978-02-15

Family

ID=10150865

Family Applications (1)

Application Number Title Priority Date Filing Date
GB20740/76A Expired GB1501563A (en) 1976-05-19 1976-05-19 Biosynthesis of microbial insecticides

Country Status (1)

Country Link
GB (1) GB1501563A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007063A2 (en) * 1990-10-10 1992-04-30 Costar Corporation A culture device having a detachable cell or tissue growth surface
CN114456998A (en) * 2022-03-23 2022-05-10 湖北天惠生物科技有限公司 Production process of easily-crushed solid culture medium for bacillus thuringiensis biopesticide

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007063A2 (en) * 1990-10-10 1992-04-30 Costar Corporation A culture device having a detachable cell or tissue growth surface
WO1992007063A3 (en) * 1990-10-10 1992-05-29 Costar Corp A culture device having a detachable cell or tissue growth surface
US5272083A (en) * 1990-10-10 1993-12-21 Costar Corporation Culture device and method of use having a detachable cell or tissue growth surface
CN114456998A (en) * 2022-03-23 2022-05-10 湖北天惠生物科技有限公司 Production process of easily-crushed solid culture medium for bacillus thuringiensis biopesticide

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee