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FR2812306B1 - Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles - Google Patents

Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles

Info

Publication number
FR2812306B1
FR2812306B1 FR0010029A FR0010029A FR2812306B1 FR 2812306 B1 FR2812306 B1 FR 2812306B1 FR 0010029 A FR0010029 A FR 0010029A FR 0010029 A FR0010029 A FR 0010029A FR 2812306 B1 FR2812306 B1 FR 2812306B1
Authority
FR
France
Prior art keywords
pca
plate
chain amplification
polymersis
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
FR0010029A
Other languages
English (en)
Other versions
FR2812306A1 (fr
Inventor
Gabriel Festoc
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR0010029A priority Critical patent/FR2812306B1/fr
Application filed by Individual filed Critical Individual
Priority to BR0112789-6A priority patent/BR0112789A/pt
Priority to DK10177401.6T priority patent/DK2269738T3/da
Priority to EA200300203A priority patent/EA004719B1/ru
Priority to ES01956628T priority patent/ES2372027T3/es
Priority to EP10177401A priority patent/EP2269738B1/fr
Priority to AT01956628T priority patent/ATE532583T1/de
Priority to EP01956628A priority patent/EP1305115B1/fr
Priority to PCT/FR2001/002385 priority patent/WO2002009877A1/fr
Priority to AU7855401A priority patent/AU7855401A/xx
Priority to AU2001278554A priority patent/AU2001278554B2/en
Priority to JP2002515419A priority patent/JP4979873B2/ja
Priority to CA002416756A priority patent/CA2416756C/fr
Priority to ES10177401T priority patent/ES2389763T3/es
Priority to CN01815876.5A priority patent/CN1248781C/zh
Priority to US09/981,070 priority patent/US6821771B2/en
Publication of FR2812306A1 publication Critical patent/FR2812306A1/fr
Priority to ZA200300700A priority patent/ZA200300700B/en
Priority to US10/926,482 priority patent/US7732136B2/en
Application granted granted Critical
Publication of FR2812306B1 publication Critical patent/FR2812306B1/fr
Priority to JP2011118127A priority patent/JP5202686B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/54—Heating or cooling apparatus; Heat insulating devices using spatial temperature gradients

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Saccharide Compounds (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
FR0010029A 2000-07-28 2000-07-28 Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles Expired - Lifetime FR2812306B1 (fr)

Priority Applications (19)

Application Number Priority Date Filing Date Title
FR0010029A FR2812306B1 (fr) 2000-07-28 2000-07-28 Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles
JP2002515419A JP4979873B2 (ja) 2000-07-28 2001-07-20 標的核酸配列の熱依存性連鎖増幅のための装置
EA200300203A EA004719B1 (ru) 2000-07-28 2001-07-20 Устройство для термозависимой цепной амплификации последовательностей - мишеней нуклеиновых кислот
ES01956628T ES2372027T3 (es) 2000-07-28 2001-07-20 Dispositivo para la amplificación en cadena termo-dependiente de secuencias de �?cidos nucleicos diana.
EP10177401A EP2269738B1 (fr) 2000-07-28 2001-07-20 Dispositif pour l'amplification en chaîne thermo-dépendante de séquences d'acides nucléiques cibles
AT01956628T ATE532583T1 (de) 2000-07-28 2001-07-20 Vorrichtung zur pcr-amplifizierung von ziel-dna- sequenzen
EP01956628A EP1305115B1 (fr) 2000-07-28 2001-07-20 Dispositif pour l'amplification en chaine thermo-dependante de sequences d'acides nucleiques cibles
ES10177401T ES2389763T3 (es) 2000-07-28 2001-07-20 Dispositivo para la amplificación de la reacción en cadena termo-dependiente de secuencias de ácidos nucleicos diana
DK10177401.6T DK2269738T3 (da) 2000-07-28 2001-07-20 Anordning til PCR-amplificering af mål-DNA-sekvenser
AU2001278554A AU2001278554B2 (en) 2000-07-28 2001-07-20 Device for heat-dependent chain amplification of target nucleic acid sequences
BR0112789-6A BR0112789A (pt) 2000-07-28 2001-07-20 Cartucho de reação, dispositivo para efetuar reações enzimáticas e/ou de biologia molecular e processos de amplificação de ácido nucleico e de enchimento em sistema fechado das cámaras de reação de um cartucho
AU7855401A AU7855401A (en) 2000-07-28 2001-07-20 Device for heat-dependent chain amplification of target nucleic acid sequences
PCT/FR2001/002385 WO2002009877A1 (fr) 2000-07-28 2001-07-20 Dispositif pour l'amplification en châine thermo-dependante de sequences d'acides nucleiques cibles
CN01815876.5A CN1248781C (zh) 2000-07-28 2001-07-20 靶核酸序列的热依赖性链反应扩增装置
CA002416756A CA2416756C (fr) 2000-07-28 2001-07-20 Dispositif pour l'amplification en chaine thermo-dependante de sequences d'acides nucleiques cibles
US09/981,070 US6821771B2 (en) 2000-07-28 2001-10-15 Device for thermo-dependent chain reaction amplification of target nucleic acid sequences, measured in real-time
ZA200300700A ZA200300700B (en) 2000-07-28 2003-01-27 Device for heat-dependent chain amplification of target nucleic acid sequences.
US10/926,482 US7732136B2 (en) 2000-07-28 2004-08-25 Device for thermo-dependent chain reaction amplification of target nucleic acid sequences, measured in real-time
JP2011118127A JP5202686B2 (ja) 2000-07-28 2011-05-26 酸素反応及び／又は分子生物学反応を実施するための装置および核酸を増幅するための方法および充填のためのプロセス

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0010029A FR2812306B1 (fr) 2000-07-28 2000-07-28 Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles

Publications (2)

Publication Number Publication Date
FR2812306A1 FR2812306A1 (fr) 2002-02-01
FR2812306B1 true FR2812306B1 (fr) 2005-01-14

Family

ID=8853103

Family Applications (1)

Application Number Title Priority Date Filing Date
FR0010029A Expired - Lifetime FR2812306B1 (fr) 2000-07-28 2000-07-28 Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles

Country Status (14)

Country Link
US (2) US6821771B2 (fr)
EP (2) EP2269738B1 (fr)
JP (2) JP4979873B2 (fr)
CN (1) CN1248781C (fr)
AT (1) ATE532583T1 (fr)
AU (2) AU7855401A (fr)
BR (1) BR0112789A (fr)
CA (1) CA2416756C (fr)
DK (1) DK2269738T3 (fr)
EA (1) EA004719B1 (fr)
ES (2) ES2389763T3 (fr)
FR (1) FR2812306B1 (fr)
WO (1) WO2002009877A1 (fr)
ZA (1) ZA200300700B (fr)

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EA200300203A1 (ru) 2003-06-26
EP2269738B1 (fr) 2012-08-29
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BR0112789A (pt) 2003-09-09
EP1305115A1 (fr) 2003-05-02
CA2416756A1 (fr) 2002-02-07
US7732136B2 (en) 2010-06-08
CN1248781C (zh) 2006-04-05
JP4979873B2 (ja) 2012-07-18
ATE532583T1 (de) 2011-11-15
DK2269738T3 (da) 2013-01-02
US20050026277A1 (en) 2005-02-03
ZA200300700B (en) 2004-03-10
EP2269738A1 (fr) 2011-01-05
WO2002009877A1 (fr) 2002-02-07
CN1458866A (zh) 2003-11-26
AU7855401A (en) 2002-02-13
EA004719B1 (ru) 2004-08-26
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US6821771B2 (en) 2004-11-23
US20020081669A1 (en) 2002-06-27
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ES2389763T3 (es) 2012-10-31

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