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FR2734839B1 - Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta-7red, procede de production, souches de levures transformees, applications. - Google Patents

Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta-7red, procede de production, souches de levures transformees, applications.

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Publication number
FR2734839B1
FR2734839B1 FR9506517A FR9506517A FR2734839B1 FR 2734839 B1 FR2734839 B1 FR 2734839B1 FR 9506517 A FR9506517 A FR 9506517A FR 9506517 A FR9506517 A FR 9506517A FR 2734839 B1 FR2734839 B1 FR 2734839B1
Authority
FR
France
Prior art keywords
delta
protein
thaliana
reductase
applications
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
FR9506517A
Other languages
English (en)
Other versions
FR2734839A1 (fr
Inventor
Xavier Chenivesse
Catherine Duport
Eric Lecain
Denis Pompon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Aventis France
Original Assignee
Roussel Uclaf SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roussel Uclaf SA filed Critical Roussel Uclaf SA
Priority to FR9506517A priority Critical patent/FR2734839B1/fr
Priority to IL11687296A priority patent/IL116872A/en
Priority to IL16247696A priority patent/IL162476A/en
Priority to US08/601,435 priority patent/US5759801A/en
Priority to SK188-96A priority patent/SK283656B6/sk
Priority to UA96020528A priority patent/UA72172C2/uk
Priority to CN961060778A priority patent/CN1216995C/zh
Priority to RU96102857/13A priority patent/RU2242517C2/ru
Priority to AT96400301T priority patent/ATE457354T1/de
Priority to CN2004100488331A priority patent/CN1664107A/zh
Priority to DE69638123T priority patent/DE69638123D1/de
Priority to KR1019960003564A priority patent/KR100422293B1/ko
Priority to EP96400301A priority patent/EP0727489B1/fr
Priority to SI9630775T priority patent/SI0727489T1/sl
Priority to BRPI9600729-0 priority patent/BRPI9600729B8/pt
Priority to ARP960101380A priority patent/AR003407A1/es
Priority to CZ1996432A priority patent/CZ294860B6/cs
Priority to DK96400301.6T priority patent/DK0727489T3/da
Priority to CA2169524A priority patent/CA2169524C/fr
Priority to FI960663A priority patent/FI120877B/fi
Priority to ES96400301T priority patent/ES2339833T3/es
Priority to PT96400301T priority patent/PT727489E/pt
Priority to HU9600325A priority patent/HU220587B1/hu
Priority to JP05079896A priority patent/JP4082469B2/ja
Priority to TR96/00116A priority patent/TR199600116A2/xx
Priority to PL96312828A priority patent/PL185569B1/pl
Priority to HR960078A priority patent/HRP960078B1/xx
Publication of FR2734839A1 publication Critical patent/FR2734839A1/fr
Priority to US08/783,202 priority patent/US5989881A/en
Application granted granted Critical
Publication of FR2734839B1 publication Critical patent/FR2734839B1/fr
Priority to US08/931,047 priority patent/US5965417A/en
Priority to IL16247704A priority patent/IL162477A0/xx
Priority to IL16247604A priority patent/IL162476A0/xx
Priority to ARP060102688A priority patent/AR053931A2/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/02—Dehydrogenating; Dehydroxylating

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
FR9506517A 1995-02-15 1995-06-01 Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta-7red, procede de production, souches de levures transformees, applications. Expired - Lifetime FR2734839B1 (fr)

Priority Applications (32)

Application Number Priority Date Filing Date Title
FR9506517A FR2734839B1 (fr) 1995-06-01 1995-06-01 Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta-7red, procede de production, souches de levures transformees, applications.
IL11687296A IL116872A (en) 1995-02-15 1996-01-23 Delta-5, 7 sterol reductase from arabidopsis thaliana
IL16247696A IL162476A (en) 1995-02-15 1996-01-23 Arabidopsis thaliana proteins having delta -5,7- sterol - delta -7- reductase activity
US08/601,435 US5759801A (en) 1995-02-15 1996-02-12 Δ5,7 -sterol reductase from arabidopsis thaliana
UA96020528A UA72172C2 (uk) 1995-02-15 1996-02-13 Послідовність нуклеїнової кислоти, що має дельта-5,7 стерин, дельта-7 редуктазну активність, спосіб одержання білка, спосіб відновлення стеринів, ненасичених в положенні с-7, спосіб одержання прегненолону, трансформований штам saccaromyces cerevisiae, що накопичує вільний та етерифікований прегненолон та спосіб виявлення недостатності дельта-5,7 стерин, дельта-7 редуктази
SK188-96A SK283656B6 (sk) 1995-02-15 1996-02-13 Sekvencia DNA kódujúca proteín organizmu A. thaliana s aktivitou delta-5,7-sterol, delta-7-reduktázy, proteín delta-7-Red, spôsob ich výroby, kmene transformovaných kvasiniek, aplikácie
PT96400301T PT727489E (pt) 1995-02-15 1996-02-14 Sequência de adn codificante para uma proteína de a. thaliana tendo uma actividade redutase em delta-7 do esterol delta-5,7, proteína delta-7-red, processo de produção, estirpes de leveduras transformadas, utilizações
AT96400301T ATE457354T1 (de) 1995-02-15 1996-02-14 Für ein a. thaliana protein kodierende dna- sequenz mit delta-5,7-sterol-delta-7- reduktaseaktivität, das protein, verfahren zur herstellung, transformierte hefe-stämme und verwendungen
CN2004100488331A CN1664107A (zh) 1995-02-15 1996-02-14 Dna序列、其编码的蛋白质及制法、转化酵母菌株及用途
DE69638123T DE69638123D1 (de) 1995-02-15 1996-02-14 Für ein A. thaliana Protein kodierende DNA-Sequenz mit Delta-5,7-sterol-delta-7-Reduktaseaktivität, das Protein, Verfahren zur Herstellung, transformierte Hefe-Stämme und Verwendungen
KR1019960003564A KR100422293B1 (ko) 1995-02-15 1996-02-14 델타-5,7스테롤,델타-7리덕타제활성을갖는에이.탈리아나의단백질을코딩하는dna서열,델타-7레드단백질,그제조방법,형질전환된효모균주및그용도
EP96400301A EP0727489B1 (fr) 1995-02-15 1996-02-14 Séquence d'ADN codant pour une protéine d'A. thaliana ayant une activité delta-5,7 stérol, delta-7 réductase, protéine delta-7-Red, procédé de production, souches de levures transformées, applications
SI9630775T SI0727489T1 (sl) 1995-02-15 1996-02-14 DNA sekvenca ki kodira protein A thaliane z aktivnostjo delta sterol delta reduktaze protein delta Red postopek za pripravo transformirani sevi kvasovk in uporabe
BRPI9600729-0 BRPI9600729B8 (pt) 1995-02-15 1996-02-14 vetor de expressão, microorganismo transgênico, processo de clonagem de um ácido nucléico e processo para preparação de uma proteína e cepa de levedura transformada
ARP960101380A AR003407A1 (es) 1995-02-15 1996-02-14 Procedimiento de clonacion de un acido nucleico que codifica una proteina con actividad de delta-5,7-esterol, delta-7-reductasa, procedimiento de preparacion de la proteina, procemiento de reduccion in vitro de un esterol insaturado, procedimiento de produccion de pregnenolona, cepa de levadura transformada y metodo de diagnostico in vitro para la deteccion de la deficiencia de delta-5, 7-esterol, delta-7-reductasa
CZ1996432A CZ294860B6 (cs) 1995-02-15 1996-02-14 Sekvence DNA kódující protein A. thaliana s aktivitou delta-5,7 sterol, delta-7 reduktasy, protein delta-7 Red, primer, protein A. thaliana, způsob jejich výroby, kmenové struktury transformovaných kvasinek a použití
CN961060778A CN1216995C (zh) 1995-02-15 1996-02-14 编码具有δ－5,7甾醇,δ－7还原酶活性的蛋白质的DNA序列和该蛋白质及生产方法，转化酵母菌株，用途
CA2169524A CA2169524C (fr) 1995-02-15 1996-02-14 Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta-7-red, procede de production, souches de levures transformees, applications
FI960663A FI120877B (fi) 1995-02-15 1996-02-14 DNA-sekvenssi, joka koodaa A. thaliana -proteiinia, jolla on delta-5,7-steroli, delta-7-reduktaasiaktiivisuutta, delta-7-Red-proteiinia, menetelmä sen valmistamiseksi, muunnettuja hiivakantoja sekä sovellutuksia
ES96400301T ES2339833T3 (es) 1995-02-15 1996-02-14 Secuencia de adn que codifica una proteina de a. thaliana que tiene una actividad delta-5,7-esterol-delta-7-reductasa, la proteina delta-7-red, procedimiento de produccion, las cepas de levaduras transformadas, aplicaciones.
RU96102857/13A RU2242517C2 (ru) 1995-02-15 1996-02-14 Последовательность нуклеиновой кислоты, кодирующая белок, обладающий дельта-5,7 стерин, дельта-7 редуктазной активностью (варианты), белок из arabidopsis thaliana, обладающий указанной активностью (варианты), вектор экспрессии, способ клонирования нуклеиновой кислоты, диагностический зонд, способ получения белка, способы восстановления стерина (варианты), способ получения прегненолона (варианты), трансформированный дрожжевой штамм и способ выявления недостаточности дельта-5,7 стерин, дельта-7 редуктазы
HU9600325A HU220587B1 (hu) 1995-02-15 1996-02-14 A delta-7-Red elnevezésű A. thaliana fehérjét kódoló DNS-szekvencia, a delta-7-Red-fehérje, eljárás előállításukra, a szekvenciával transzformált élesztőtörzsek, és felhasználásuk
DK96400301.6T DK0727489T3 (da) 1995-02-15 1996-02-14 DNA-sekvens, der koder for et A. thaliana protein med delta-5,7-sterol, delta-7-reduktase-aktivitet, delta-7Red-protein, fremgangsmåde til fremstilling, transformerede gærstammer, anvendelser
JP05079896A JP4082469B2 (ja) 1995-02-15 1996-02-15 デルタ−５，７ステロール，デルタ−７レダクターゼ活性を有するＡ．Ｔｈａｌｉａｎａの蛋白質をコードするＤＮＡ配列、デルタ７−Ｒｅｄ蛋白質、製造方法、トランスフォームした酵母株、及び利用
TR96/00116A TR199600116A2 (tr) 1995-02-15 1996-02-15 Bir delta-5,7 sterol, delta-7 reduktaz aktivitesi, delta 7-red proteinine sahip bir a. talian proteini icin dna sirasi kodlamasi, üretim islemi, dönüstürülmüs maya türü, kullanimlari.
PL96312828A PL185569B1 (pl) 1995-02-15 1996-02-15 Sekwencje kwasu nukleinowego kodujące białko o aktywności delta-7 reduktazy delta-5,7-sterolowej, sposób klonowania takiego kwasu nukleinowego, białko o aktywności delta-7 reduktazy delta-5,7-sterolowej i białko A. thaliana, sposób wytwarzania białka i sposób wytwarzania pregnenolonu, sposób redukcji in vivo i in vitro steroli nienasyconych w pozycji C-7, przeciwciało, wektor ekspresyjny, stransformowany szczep drożdży, stransformowane komórki gospodarza i sposób detekcji niedoboru delta-7 reduktazy delta-5,7-sterolowej
HR960078A HRP960078B1 (en) 1995-02-15 1996-02-15 Dna sequence coding for a protein of a. thaliana having a delta-5, 7 sterol, delta- 7 reductase activity, delta-7-red protein, production process, strains of transformed yeasts, uses
US08/783,202 US5989881A (en) 1995-02-15 1997-01-14 Nucleic acid molecules encoding delta-5,7-sterol, delta-7 reductase from arabidopsis thaliana
US08/931,047 US5965417A (en) 1995-02-15 1997-09-16 Arabidepsi thaliana proteins having Δ-5,7-sterol-Δ-7-reductase activity
IL16247604A IL162476A0 (en) 1995-02-15 2004-06-10 Arabidopsi thaliana proteins havingdelta-5,7-sterol-delta-7-reductase activity
IL16247704A IL162477A0 (en) 1995-02-15 2004-06-10 Nucleic acid molecules encoding delta-5,7-sterol, delta-7 reductase from arabidopsis thaliana
ARP060102688A AR053931A2 (es) 1995-02-15 2006-06-22 Secuencia de adn que codifica una proteina de a. thaliana que posee actividad de delta -5,7-esterol, delta-7-reductasa, proteina delta-7red

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9506517A FR2734839B1 (fr) 1995-06-01 1995-06-01 Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta-7red, procede de production, souches de levures transformees, applications.

Publications (2)

Publication Number Publication Date
FR2734839A1 FR2734839A1 (fr) 1996-12-06
FR2734839B1 true FR2734839B1 (fr) 1997-07-04

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Family Applications (1)

Application Number Title Priority Date Filing Date
FR9506517A Expired - Lifetime FR2734839B1 (fr) 1995-02-15 1995-06-01 Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta-7red, procede de production, souches de levures transformees, applications.

Country Status (1)

Country Link
FR (1) FR2734839B1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112280758B (zh) * 2019-11-18 2024-07-19 沈阳博泰生物制药有限公司 一种甾体5β还原酶变体及其用途
CN113151317A (zh) * 2021-05-26 2021-07-23 云南中烟工业有限责任公司 一种烟草δ（24）-固醇还原酶基因及其应用

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