FR2731015B1 - Procede d'enrichissement enzymatique d'huiles d'origine marine et les triglycerides d'acides gras polyinsatures ainsi obtenus - Google Patents
Procede d'enrichissement enzymatique d'huiles d'origine marine et les triglycerides d'acides gras polyinsatures ainsi obtenusInfo
- Publication number
- FR2731015B1 FR2731015B1 FR9502153A FR9502153A FR2731015B1 FR 2731015 B1 FR2731015 B1 FR 2731015B1 FR 9502153 A FR9502153 A FR 9502153A FR 9502153 A FR9502153 A FR 9502153A FR 2731015 B1 FR2731015 B1 FR 2731015B1
- Authority
- FR
- France
- Prior art keywords
- triglycerides
- oils
- fatty acids
- polyunsaturated fatty
- marine origin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6432—Eicosapentaenoic acids [EPA]
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
- C11C1/00—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids
- C11C1/02—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids from fats or fatty oils
- C11C1/04—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids from fats or fatty oils by hydrolysis
- C11C1/045—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids from fats or fatty oils by hydrolysis using enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
- C11C3/00—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
- C11C3/02—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fatty acids with glycerol
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
- C11C3/00—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
- C11C3/04—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fats or fatty oils
- C11C3/10—Ester interchange
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6434—Docosahexenoic acids [DHA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6481—Phosphoglycerides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
Priority Applications (12)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9502153A FR2731015B1 (fr) | 1995-02-24 | 1995-02-24 | Procede d'enrichissement enzymatique d'huiles d'origine marine et les triglycerides d'acides gras polyinsatures ainsi obtenus |
EP96904125A EP0784694A1 (fr) | 1995-02-24 | 1996-02-13 | Procedes enzymatiques d'enrichissement en acides gras polyinsatures |
US08/737,098 US6537787B1 (en) | 1995-02-24 | 1996-02-13 | Enzymatic methods for polyunsaturated fatty acid enrichment |
PCT/FR1996/000226 WO1996026287A1 (fr) | 1995-02-24 | 1996-02-13 | Procedes enzymatiques d'enrichissement en acides gras polyinsatures |
MA24163A MA23806A1 (fr) | 1995-02-24 | 1996-02-15 | Procedes enzymatiques d'enrichissement en acides gras polyinsatures |
TNTNSN96031A TNSN96031A1 (fr) | 1995-02-24 | 1996-02-21 | Procedes enzymatiques d'enrichissement en acides gras polyinsatures |
IL11725196A IL117251A (en) | 1995-02-24 | 1996-02-23 | Enzymatic process for obtaining polyunsaturated fatty acid triglyceride concentrates |
MYPI96000665A MY115719A (en) | 1995-02-24 | 1996-02-23 | Enzymatic processes for enrichment with polyunsaturated fatty acids |
DZ960038A DZ1997A1 (fr) | 1995-02-24 | 1996-02-24 | Procédé enzymatique d'enrichissement en acides polyinsatures. |
PE1996000264A PE51497A1 (es) | 1995-02-24 | 1996-04-16 | Procedimientos enzimaticos de enriquecimiento en acidos grasos poli-insaturados |
IL12523198A IL125231A0 (en) | 1995-02-24 | 1998-07-06 | Enzymatic process for obtaining monoacylglycerides of polyunsaturated fatty acids |
IL12523098A IL125230A0 (en) | 1995-02-24 | 1998-07-06 | Enzymatic process for enrichment with polyunsaturated fatty acids |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9502153A FR2731015B1 (fr) | 1995-02-24 | 1995-02-24 | Procede d'enrichissement enzymatique d'huiles d'origine marine et les triglycerides d'acides gras polyinsatures ainsi obtenus |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2731015A1 FR2731015A1 (fr) | 1996-08-30 |
FR2731015B1 true FR2731015B1 (fr) | 1997-05-30 |
Family
ID=9476469
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR9502153A Expired - Lifetime FR2731015B1 (fr) | 1995-02-24 | 1995-02-24 | Procede d'enrichissement enzymatique d'huiles d'origine marine et les triglycerides d'acides gras polyinsatures ainsi obtenus |
Country Status (10)
Country | Link |
---|---|
US (1) | US6537787B1 (fr) |
EP (1) | EP0784694A1 (fr) |
DZ (1) | DZ1997A1 (fr) |
FR (1) | FR2731015B1 (fr) |
IL (1) | IL117251A (fr) |
MA (1) | MA23806A1 (fr) |
MY (1) | MY115719A (fr) |
PE (1) | PE51497A1 (fr) |
TN (1) | TNSN96031A1 (fr) |
WO (1) | WO1996026287A1 (fr) |
Families Citing this family (47)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030054509A1 (en) * | 2001-04-06 | 2003-03-20 | Archer-Daniels-Midland Company | Method for producing fats or oils |
AU2003222739A1 (en) * | 2002-05-08 | 2003-11-11 | Danmarks Tekniske Universitet (Technical University Of Denmark) | A facile two-step enzyme process for increasing the content of polyunsaturated fatty acids in fish oil |
FR2839887A1 (fr) | 2002-05-22 | 2003-11-28 | Synergia | Nouvelles compositions nutraceutiques et pharmaceutiques et leurs utilisations |
DK175501B1 (da) * | 2002-12-02 | 2004-11-15 | Green Earth | Anlæg og fremgangsmåde til kontinuerlig hydrolyse af et proteinholdigt animalsk eller vegetabilsk råmateriale |
GB0300602D0 (en) * | 2003-01-10 | 2003-02-12 | Forinnova As | Compositions |
WO2005040334A2 (fr) * | 2003-10-23 | 2005-05-06 | Enzymotec Ltd. | Lipases selectives et methodes de criblage de celles-ci |
US20070251141A1 (en) * | 2004-02-26 | 2007-11-01 | Purdue Research Foundation | Method for Preparation, Use and Separation of Fatty Acid Esters |
US20050232956A1 (en) * | 2004-02-26 | 2005-10-20 | Shailendra Bist | Method for separating saturated and unsaturated fatty acid esters and use of separated fatty acid esters |
JP2007526943A (ja) * | 2004-03-04 | 2007-09-20 | エイチティーエル ハイ − テック リピッズ リミテッド | 構造化されたトリグリセリド類およびそれを含むエマルション類 |
US7972831B2 (en) * | 2004-10-08 | 2011-07-05 | Novozymes A/S | Lipases from thermophilic anaerobes |
US20060177486A1 (en) * | 2004-11-17 | 2006-08-10 | Natural Asa | Enzymatically synthesized marine phospholipids |
WO2006102896A2 (fr) * | 2005-03-29 | 2006-10-05 | Aalborg Universitet | Procede servant a concentrer $g(v)3-pufa a partir d'huile de poisson par hydrolyse selective au moyen de cutinase immobilisee sur nay zeolitique |
US7691270B2 (en) * | 2005-07-13 | 2010-04-06 | Battelle Energy Alliance, Llc | Method for removing impurities from an impurity-containing fluid stream |
ES2277557B1 (es) | 2005-12-21 | 2008-07-01 | Proyecto Empresarial Brudy, S.L. | Utilizacion de acido docosahexaenoico para el tratamiento del daño celular oxidativo. |
JP2009523414A (ja) * | 2005-12-21 | 2009-06-25 | ブルーディ、テクノロジー、ソシエダッド、リミターダ | 細胞の酸化的損傷に関連した病変を治療するためのdha、epaまたはdha由来のepaの使用 |
WO2008149177A2 (fr) * | 2006-05-05 | 2008-12-11 | Natural Asa | Compositions lipidiques marines et leurs utilisations |
ITMI20070157A1 (it) * | 2007-01-31 | 2008-08-01 | Adorkem Technology Spa | Processo di arricchimento enzimatico di una miscela contenente omega 3 |
US20090199462A1 (en) * | 2007-03-23 | 2009-08-13 | Shailendra Bist | Method for separating saturated and unsaturated fatty acid esters and use of separated fatty acid esters |
US8697138B2 (en) | 2007-03-28 | 2014-04-15 | Aker Biomarine As | Methods of using krill oil to treat risk factors for cardiovascular, metabolic, and inflammatory disorders |
DK2144618T4 (da) | 2007-03-28 | 2025-02-03 | Aker Biomarine Antarctic As | Bioeffektive sammensætninger af krillolie |
EP1978102B1 (fr) | 2007-04-02 | 2010-07-07 | Cognis IP Management GmbH | Un mélange comprenant des glycérides d'acides gras |
EP1978101A1 (fr) | 2007-04-02 | 2008-10-08 | Cognis IP Management GmbH | Procédé d'enrichissement en acides gras polyinsaturés |
US20100226977A1 (en) * | 2007-08-29 | 2010-09-09 | Aker Biomarine Asa | Low viscosity phospholipid compositions |
CA2839075A1 (fr) * | 2007-08-29 | 2009-03-05 | Aker Biomarine Asa | Procede inedit de fabrication de farine de krill |
US20090182049A1 (en) * | 2008-01-16 | 2009-07-16 | Joar Arild Opheim | Pharmaceutical Composition and Method for Treating Hypertriglyceridemia and Hypercholesterolemia in Humans |
US8308954B2 (en) * | 2008-09-25 | 2012-11-13 | Battelle Energy Alliance, Llc | Methods for recovering a polar solvent from a fluid stream contaminated with at least one polar impurity |
US8747673B2 (en) | 2008-09-25 | 2014-06-10 | Battelle Energy Alliance, Llc | Methods for recovering a solvent from a fluid volume and methods of removing at least one compound from a nonpolar solvent |
US8372812B2 (en) * | 2009-02-26 | 2013-02-12 | Aker Biomarine Asa | Phospholipid and protein tablets |
US20110223246A1 (en) * | 2010-03-10 | 2011-09-15 | Joar Opheim | Docosahexaenoic acid bound in phospholipids and method of recovering same from a natural source |
EP3384902B1 (fr) | 2012-02-17 | 2020-12-30 | Alcresta Therapeutics, Inc. | Procédés, compositions et dispositifs pour répondre aux besoins en acides gras alimentaires |
EP2978307B1 (fr) | 2013-03-28 | 2018-12-26 | The Trustees of Columbia University in the City of New York | La reperfusion par des glycérides oméga-3 favorise la protection d'un organe de donneur pour la greffe |
AU2014203179C1 (en) | 2013-06-14 | 2017-05-04 | Aker Biomarine Antarctic As | Lipid extraction processes |
GB201400431D0 (en) | 2014-01-10 | 2014-02-26 | Aker Biomarine As | Phospholipid compositions and their preparation |
BR112016028561A8 (pt) | 2014-06-06 | 2021-06-29 | Marine Ingredients Llc | composição e forma de dosagem compreendendo um óleo marinho que compreende ácidos graxos, e, uso das mesmas |
KR101970151B1 (ko) * | 2014-10-21 | 2019-04-19 | 유니버시다드 데 레스 일레스 발레아르스 | 하이드록시-트리글리세라이드의 합성 방법 및 질병의 예방 및 치료에 사용되는 하이드록시-트리글리세라이드의 용도 |
FR3029738B1 (fr) * | 2014-12-10 | 2016-12-30 | Agronutrition | Procede de preparation d'une composition de traitement de plante, composition obtenue et ses utilisations |
CN105779140A (zh) * | 2014-12-23 | 2016-07-20 | 浙江医药股份有限公司新昌制药厂 | 一种高含量epa乙酯型鱼油的制备方法 |
WO2016128830A1 (fr) | 2015-02-11 | 2016-08-18 | Aker Biomarine Antarctic As | Procédés d'extraction de lipides |
KR102515207B1 (ko) | 2015-02-11 | 2023-03-29 | 에이커 바이오마린 앤탁틱 에이에스 | 지질 조성물 |
EP3789476B1 (fr) * | 2015-05-13 | 2024-11-27 | Epax Norway AS | Methode de production d'acides gras polyinsaturés à très longue chaîne fabriqués à partir d'huiles naturelles et compositions nutraceutiques obtenues. |
GB2538758A (en) * | 2015-05-27 | 2016-11-30 | Green Lizard Tech Ltd | Process for removing chloropropanols and/or glycidol |
EP3317385B1 (fr) | 2015-07-02 | 2019-04-03 | Stepan Company | Procédé de préparation de 2-monoacylglycérides |
US10258590B2 (en) | 2015-10-14 | 2019-04-16 | Alcresta Therapeutics, Inc. | Enteral feeding device and related methods of use |
CN106609286B (zh) * | 2015-10-22 | 2022-01-25 | 丰益(上海)生物技术研发中心有限公司 | 含长链多不饱和脂肪酸的磷脂的制备方法 |
CN110468166A (zh) * | 2019-08-26 | 2019-11-19 | 中国科学院深圳先进技术研究院 | 甘油酯型鱼油的制备方法、浓缩型甘油酯型鱼油和鱼油制品 |
CN115478084B (zh) * | 2021-06-15 | 2025-02-07 | 嘉必优生物技术(武汉)股份有限公司 | 一种多不饱和脂肪酸酯的制备方法 |
WO2023106793A1 (fr) * | 2021-12-07 | 2023-06-15 | Chong Kun Dang Pharmaceutical Corp. | Procédé de production de dérivés de triglycérides de haute pureté contenant une teneur élevée en acide eicosapentanoïque (epa) |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60234588A (ja) * | 1984-05-07 | 1985-11-21 | Asahi Denka Kogyo Kk | 長鎖高度不飽和脂肪酸アルコ−ルエステルの製造法 |
JPS60234589A (ja) * | 1984-05-07 | 1985-11-21 | Asahi Denka Kogyo Kk | 油脂の製造法 |
JPH0645570B2 (ja) * | 1984-08-03 | 1994-06-15 | 日清製粉株式会社 | エイコサペンタエン酸のグリセリンエステルの製法 |
JPH0225447A (ja) * | 1988-07-13 | 1990-01-26 | Nippon Oil & Fats Co Ltd | 高度不飽和脂肪酸類の製造方法 |
DK190689D0 (da) * | 1989-04-19 | 1989-04-19 | Novo Industri As | Omestringsproces |
GB2236537A (en) * | 1989-09-13 | 1991-04-10 | Unilever Plc | Transesterification |
JPH03108489A (ja) * | 1989-09-22 | 1991-05-08 | Meito Sangyo Kk | 長鎖高度不飽和脂肪酸モノグリセリドの製造法 |
AU6785490A (en) * | 1989-12-18 | 1991-06-20 | Kraft General Foods, Inc. | Low-saturate edible oils and transesterification methods for production thereof |
JP2516860B2 (ja) * | 1991-10-03 | 1996-07-24 | 工業技術院長 | 濃縮された高度不飽和脂肪酸含有油脂の製造方法 |
JPH06217783A (ja) * | 1993-01-28 | 1994-08-09 | Maruha Corp | 長鎖高度不飽和脂肪酸を高純度に含むトリグリセリドの製造方法 |
GB9404483D0 (en) * | 1994-03-08 | 1994-04-20 | Norsk Hydro As | Refining marine oil compositions |
-
1995
- 1995-02-24 FR FR9502153A patent/FR2731015B1/fr not_active Expired - Lifetime
-
1996
- 1996-02-13 US US08/737,098 patent/US6537787B1/en not_active Expired - Fee Related
- 1996-02-13 EP EP96904125A patent/EP0784694A1/fr not_active Withdrawn
- 1996-02-13 WO PCT/FR1996/000226 patent/WO1996026287A1/fr not_active Application Discontinuation
- 1996-02-15 MA MA24163A patent/MA23806A1/fr unknown
- 1996-02-21 TN TNTNSN96031A patent/TNSN96031A1/fr unknown
- 1996-02-23 IL IL11725196A patent/IL117251A/xx not_active IP Right Cessation
- 1996-02-23 MY MYPI96000665A patent/MY115719A/en unknown
- 1996-02-24 DZ DZ960038A patent/DZ1997A1/fr active
- 1996-04-16 PE PE1996000264A patent/PE51497A1/es not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
EP0784694A1 (fr) | 1997-07-23 |
TNSN96031A1 (fr) | 2005-03-15 |
IL117251A0 (en) | 1996-06-18 |
WO1996026287A1 (fr) | 1996-08-29 |
PE51497A1 (es) | 1997-12-24 |
DZ1997A1 (fr) | 2002-07-20 |
FR2731015A1 (fr) | 1996-08-30 |
MA23806A1 (fr) | 1996-10-01 |
MY115719A (en) | 2003-08-30 |
IL117251A (en) | 2000-09-28 |
US6537787B1 (en) | 2003-03-25 |
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