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FR2603105A1 - Method and device for local and instantaneous measurement of the absolute concentration of a chemical species in a reactive medium - Google Patents

Method and device for local and instantaneous measurement of the absolute concentration of a chemical species in a reactive medium

Info

Publication number
FR2603105A1
FR2603105A1 FR8611857A FR8611857A FR2603105A1 FR 2603105 A1 FR2603105 A1 FR 2603105A1 FR 8611857 A FR8611857 A FR 8611857A FR 8611857 A FR8611857 A FR 8611857A FR 2603105 A1 FR2603105 A1 FR 2603105A1
Authority
FR
France
Prior art keywords
reactive medium
chemical species
absolute concentration
fluorescence
local
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR8611857A
Other languages
French (fr)
Other versions
FR2603105B1 (en
Inventor
Denis Stepowski
Leon Stepowski
Pierre Stepowski
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Priority to FR8611857A priority Critical patent/FR2603105B1/en
Publication of FR2603105A1 publication Critical patent/FR2603105A1/en
Application granted granted Critical
Publication of FR2603105B1 publication Critical patent/FR2603105B1/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence

Landscapes

  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Optics & Photonics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

SELON L'INVENTION ON FAIT SE PROPAGER DANS LE MILIEU REACTIF 3 UN PREMIER FAISCEAU LASER 1 ISSU D'UN GENERATEUR LASER 101 ACCORDE SUR UNE RAIE D'ABSORPTION DE L'ESPECE A MESURER, SELON UNE DIRECTION PREDETERMINEE X X ET DANS UN PREMIER SENS, ET UN SECOND FAISCEAU LASER 2 ISSU DU MEME GENERATEUR LASER 101 ACCORDE, MAIS PRESENTANT UN FAIBLE RETARD PREDETERMINE, SELON LADITE DIRECTION PREDETERMINEE, ET DANS UN SENS OPPOSE AUDIT PREMIER SENS, ON RECUEILLE LE LONG DE LADITE DIRECTION PREDETERMINEE, EN DEUX POINTS DISTINCTS A, B DU MILIEU REACTIF 3 TRES PROCHES L'UN DE L'AUTRE, UNE PARTIE DE L'ENERGIE LUMINEUSE QUI A ETE ABSORBEE LE LONG DES FAISCEAUX D'EXCITATION 1, 2 ET A PROVOQUE L'EMISSION DE FLUORESCENCE, ON MESURE LA FLUORESCENCE EMISE PAR LES DEUX POINTS DISTINCTS A, B POUR DETERMINER POUR CHACUN DE CES DEUX POINTS A, B UNE PAIRE DE SIGNAUX DE FLUORESCENCE DECALES DANS LE TEMPS, ON DEDUIT DES MESURES DES DEUX PAIRES DE SIGNAUX DE FLUORESCENCE LA CONCENTRATION ABSOLUE DE L'ESPECE CHIMIQUE ABSORBANTE PRESENTE DANS LE MILIEU REACTIF 3.ACCORDING TO THE INVENTION, A FIRST LASER BEAM 1 IS PROPAGATED IN THE REACTIVE MEDIUM 3 FROM A LASER GENERATOR 101 GRANTED ON AN ABSORPTION RAY OF THE SPECIES TO BE MEASURED, ACCORDING TO A PREDETERMINED DIRECTION XX AND IN A FIRST DIRECTION, AND A SECOND LASER BEAM 2 FROM THE SAME LASER 101 GENERATOR GRANTED, BUT PRESENTING A LOW PREDETERMINED DELAY, ACCORDING TO THE SAID PREDETERMINED DIRECTION, AND IN AN OPPOSITE TO AUDIT FIRST DIRECTION, WE COLLECT ALONG THE LADITE PREDETERMINED DIRECTION, SEPARATELY POINT, B OF THE REACTIVE MEDIUM 3 VERY CLOSE TO ONE THE OTHER, PART OF THE LUMINOUS ENERGY WHICH WAS ABSORBED ALONG THE EXCITATION BEAMS 1, 2 AND CAUSED THE EMISSION OF FLUORESCENCE, THE FLUORESCENCE EMITTED IS MEASURED BY THE TWO DISTINCT POINTS A, B TO DETERMINE FOR EACH OF THESE TWO POINTS A, B A PAIR OF FLUORESCENCE SIGNALS SHIFTED IN TIME, WE DEDUCT FROM THE MEASUREMENTS OF THE TWO PAIRS OF FLUORESCENCE SIGNALS THE ABSOLUTE CONCENTRATION OF THE CHEMICAL SPECIES THAT ABSORBENT PRESENT IN THE REACTIVE MEDIUM 3.

FR8611857A 1986-08-19 1986-08-19 METHOD AND DEVICE FOR LOCAL AND INSTANT MEASUREMENT OF ABSOLUTE CONCENTRATION OF CHEMICAL SPECIES IN A REACTIVE MEDIUM Expired FR2603105B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR8611857A FR2603105B1 (en) 1986-08-19 1986-08-19 METHOD AND DEVICE FOR LOCAL AND INSTANT MEASUREMENT OF ABSOLUTE CONCENTRATION OF CHEMICAL SPECIES IN A REACTIVE MEDIUM

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8611857A FR2603105B1 (en) 1986-08-19 1986-08-19 METHOD AND DEVICE FOR LOCAL AND INSTANT MEASUREMENT OF ABSOLUTE CONCENTRATION OF CHEMICAL SPECIES IN A REACTIVE MEDIUM

Publications (2)

Publication Number Publication Date
FR2603105A1 true FR2603105A1 (en) 1988-02-26
FR2603105B1 FR2603105B1 (en) 1988-12-02

Family

ID=9338379

Family Applications (1)

Application Number Title Priority Date Filing Date
FR8611857A Expired FR2603105B1 (en) 1986-08-19 1986-08-19 METHOD AND DEVICE FOR LOCAL AND INSTANT MEASUREMENT OF ABSOLUTE CONCENTRATION OF CHEMICAL SPECIES IN A REACTIVE MEDIUM

Country Status (1)

Country Link
FR (1) FR2603105B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0762114A2 (en) * 1995-09-07 1997-03-12 Basf Aktiengesellschaft Apparatus for parallel two-photon fluorescence-correlation-spectroscopy measurements of a plurality of samples and its application to active-substance screening

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US397155A (en) * 1889-02-05 Tooth for horse hay-rakes
US4362388A (en) * 1980-11-17 1982-12-07 Bethlehem Steel Corp. Remote measurement of concentration of a gas specie by resonance absorption

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US397155A (en) * 1889-02-05 Tooth for horse hay-rakes
US4362388A (en) * 1980-11-17 1982-12-07 Bethlehem Steel Corp. Remote measurement of concentration of a gas specie by resonance absorption

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0762114A2 (en) * 1995-09-07 1997-03-12 Basf Aktiengesellschaft Apparatus for parallel two-photon fluorescence-correlation-spectroscopy measurements of a plurality of samples and its application to active-substance screening
EP0762114A3 (en) * 1995-09-07 1998-05-06 Basf Aktiengesellschaft Apparatus for parallel two-photon fluorescence-correlation-spectroscopy measurements of a plurality of samples and its application to active-substance screening

Also Published As

Publication number Publication date
FR2603105B1 (en) 1988-12-02

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