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FR2540513A1 - Device for preserving and transporting a bacterial sample - Google Patents

Device for preserving and transporting a bacterial sample Download PDF

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Publication number
FR2540513A1
FR2540513A1 FR8302167A FR8302167A FR2540513A1 FR 2540513 A1 FR2540513 A1 FR 2540513A1 FR 8302167 A FR8302167 A FR 8302167A FR 8302167 A FR8302167 A FR 8302167A FR 2540513 A1 FR2540513 A1 FR 2540513A1
Authority
FR
France
Prior art keywords
plug
tube
plastic
medium
swab
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR8302167A
Other languages
French (fr)
Other versions
FR2540513B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SCHMORAK RAYMOND
Original Assignee
SCHMORAK RAYMOND
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SCHMORAK RAYMOND filed Critical SCHMORAK RAYMOND
Priority to FR8302167A priority Critical patent/FR2540513A1/en
Publication of FR2540513A1 publication Critical patent/FR2540513A1/en
Application granted granted Critical
Publication of FR2540513B1 publication Critical patent/FR2540513B1/fr
Granted legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/22—Means for packing or storing viable microorganisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • A61B10/0096—Casings for storing test samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • A61B10/02—Instruments for taking cell samples or for biopsy
    • A61B2010/0216—Sampling brushes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Pathology (AREA)
  • Medical Informatics (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a device which makes it possible to preserve for several hours without deterioration or proliferation a bacterial sample and to transport it to the laboratory in an aseptic manner. It consists of a brush d inserted into a cap a, which are placed and steadied by a ring b in a tube c having at its end a hollow cap f closed off by a cover e containing a medium g for preserving microorganisms. When the ring b is removed, the brush d simply has to be replaced in the tube c, the sampling having been carried out, for the microorganisms to come into contact with the preserving medium g. The device according to the invention is particularly designed for preserving and transporting bacterial samples.

Description

La présente invention concerne un dispositif pour conserver et transporter in prélèvement bactérien , en particulier lorsque celui-ci ne peut 8tre traité ou remis rapidement au laboratoire charge de ltanalyse bactériologique de ce dernier. The present invention relates to a device for storing and transporting a bacterial sample, in particular when the latter cannot be treated or quickly delivered to the laboratory responsible for the bacteriological analysis of the latter.

La conservation et le transport des prélèvements bac- atériens esi; traditionnellement assuré par divers dispositifs présentant chacun un ou plusieurs inconvénients, et qui sont les suivants : - dispositifs comportant une ampoule de verre à briser avec risques de blessures ; cette ampoule étant peu visible, l'opérateur oublie parfois de la briser d'où une altération rapide du prélèvement. The conservation and the transport of the bacerial samples esi; traditionally provided by various devices each having one or more drawbacks, which are as follows: - devices comprising a glass bulb to break with risk of injury; this bulb is not very visible, the operator sometimes forgets to break it, resulting in rapid deterioration of the sample.

- dispositifs necessitant une manipulation ris- quant de compromettre l'aseptie du prélèvemente
- dispositifs comprenant un milieu Inadéquat soit à la conservation des germes, soit rendant très aléatoire l'examen direct entre lame et lamelle et la coloration par le Gram de ces germes.- devices requiring manipulation which risk compromising the aseptic nature of the sample
- devices comprising an inadequate environment either for the conservation of germs, or making the direct examination between blade and coverslip and the staining by Gram of these germs very random.

- dispositifs ne permettant pas l'accés à des
zones de prélèvement trop profondes
Le dispositif selon l'invention permet de remédier à tous ces inconv énients.- devices that do not allow access to
too deep sampling areas
The device according to the invention overcomes all these drawbacks.

La figure 1 représente le dispositif selon l' l'invention, vu en coupe, dispositif monté, avant l'emploi.  Figure 1 shows the device according to the invention, seen in section, device mounted, before use.

La figure 2 représente sa vue en coupe, après l'emploi, bague (b) otée, opercule (e) brisé et milieu (g) absorbé par l'écouv illon. Figure 2 shows its sectional view, after use, ring (b) removed, seal (e) broken and medium (g) absorbed by the illon swab.

La figure 3 représente, vu en coupe, le montage qui permet de doubler la longeur du dispositif, le tube (c) étant introduit et maintenu par friction dans la zone ereuse (a1) du bouchon (a).  FIG. 3 represents, seen in section, the assembly which makes it possible to double the length of the device, the tube (c) being introduced and maintained by friction in the erous zone (a1) of the plug (a).

La figure 4 représente, vu en coupe, le dispositif en éclatée La bandelette de papier adhésif (h) est vue à plat. Figure 4 shows, in section, the exploded device The strip of adhesive paper (h) is seen flat.

La bague fendue (b) est vue en coupe et en vue axonome- trique.The split ring (b) is seen in section and in an axonometric view.

Le dispositif, cylindrique, d'un diamètre de 20 mm environ et d'une longeur de 170 mm env iron, représenté sur ces figures, comporte un tube de plastique (c) conte- nant à son extrémité un bouchon creux (f) renfermant un milieu de conservation et de transport (g), hermétiquement
scellé par un opercule ( e) frangible. The device, cylindrical, with a diameter of about 20 mm and a length of about 170 mm iron, shown in these figures, comprises a plastic tube (c) containing at its end a hollow plug (f) containing a hermetically sealed storage and transport medium (g)
sealed with a frangible seal.

Dans ce tube plastique (c) est place un écouvillon stérile (d) solidaire d'un bouchon plastique (a) inséré dans l'autre extrémité du tube (c). In this plastic tube (c) is placed a sterile swab (d) secured to a plastic plug (a) inserted in the other end of the tube (c).

Un bague fendue (b) est maintenue et calée par une bandelette de papier adhésif (h) entre le haut du tube (c) et l'épaulement inferieur du bouchon (a) porte-écouvillon.  A split ring (b) is held and wedged by a strip of adhesive paper (h) between the top of the tube (c) and the lower shoulder of the plug (a) swab holder.

Lors de l'utilisation du dispositif, l'opérateur i3te la bande de papier adhesif (h), reture l'ensemble bouchon porte-écouvillon (a) du tube plastique (c) et de ce fait libère la bague fendue (b), qui, constituée de deux demi-seg- ments, tombe d'elle mime. When using the device, the operator i3te the strip of adhesive paper (h), retures the swab holder cap assembly (a) from the plastic tube (c) and thereby frees the split ring (b), which, made up of two half-segments, falls from itself.

L'opérateur effectue le prélèvement à l'aide de l'écou- villon (d) puis remet en place l'ensemble bouchon-porte-écou- villon (a) dans le tube (c) en appuyant à fond. The operator takes the sample using the swab (d) then replaces the plug-swab holder assembly (a) in the tube (c) by pressing fully.

La bague fendue (b) étant oyes, leëcouvillon (d) perfore l'opercule (e) du bouchon creux (f) et s'imprègne du milieu conservateur (g). The split ring (b) being oyes, the swab (d) perforates the cover (e) of the hollow plug (f) and permeates the preservative medium (g).

D ' autre part, la moitie supérieure du bouchon-porte- écouvillon (a) étant creuse, on peut emboiter le tube (e) dans cet evidement (a1), ce qui double la longeur du
disposîtf et autorise ainsi l'accés au prélèvement de zones profondes (usage vétérinaire par exemple).On the other hand, the upper half of the swab holder plug (a) being hollow, the tube (e) can be fitted into this recess (a1), which doubles the length of the
thus disposing of and authorizing access to the sampling of deep areas (veterinary use for example).

Celon une forme de réalisation préférentielle, le tube plastique (c) est obtenu par extrusion ; le bouchon-porteécouvillon (a) ainsi que le bouchon creux (f) sont en plastique moule ; la bague fendue (b) peut Btre réalisée en plastique ou en carton ; la bandelette (h) est en papier adhésif ; l'opercule (e) du bouchon creux (f) peut être réalisé en film de plastique cassant ou en feuille d'aluminium ; le milieu conservateur (g) correspond à des formules connues et utilisées en bactériologie , et sa composition peut être adaptee au transport de germes ayant des exigences particulières (exigences nutritionelles, anaerobies stricts, etc...) ; lë.cou- villon est un tampon de rayonne enroulé à l'extrémité d'un bStonnet en plastique. According to a preferred embodiment, the plastic tube (c) is obtained by extrusion; the swab holder plug (a) and the hollow plug (f) are made of molded plastic; the split ring (b) can be made of plastic or cardboard; the strip (h) is made of adhesive paper; the lid (e) of the hollow plug (f) can be made of brittle plastic film or aluminum foil; the preservative medium (g) corresponds to formulas known and used in bacteriology, and its composition can be adapted to the transport of germs having particular requirements (nutritional requirements, strict anaerobes, etc.); the cover is a rayon tampon wrapped at the end of a plastic bStonnet.

Le dispositif selon l'invention est particulièrement des tiné à la conservation et au transport de prélèvements bac atériens  The device according to the invention is particularly suitable for the conservation and transport of aerial bac samples

Claims (10)

1) Dispositif pour prelever, conserver et transporter un prelèvement bactérien, caractérisé en ce qu.f il comporte un ecouvillon (d) inséré dans un bouchon (a) place et cale par une bague (b) dans un tube (c) présentant à son extrémité un bouchon creux (f) obturé par un opercule (e) renfermant un milieu de conservation (g) de micro-organismes. 1) Device for collecting, storing and transporting a bacterial sample, characterized in that it comprises a swab (d) inserted in a plug (a) placed and wedged by a ring (b) in a tube (c) having its end a hollow plug (f) closed by a cover (e) containing a preservation medium (g) of microorganisms. REVENDICATIONS 2) Dispositif selon la revendication t caracterise en ce que le bouchon (a) possède une moitie supérieure creuse qui de par son diamètre peut être emboité sur l'une quelconque extremite du tube (c) et que ledit bouchon(a) est réalise en plastique par moulage puis evidement par alesage. 2) Device according to claim t characterized in that the plug (a) has a hollow upper half which by its diameter can be fitted on any end of the tube (c) and that said plug (a) is made in plastic by molding then recess by bore. 3) Dispositif selon la revendication t caractérisé en ce que la forme et le diamètre du bouchon (a) lui permet 3) Device according to claim t characterized in that the shape and diameter of the plug (a) allows it de maintenir fermement l'écouvillon (d) et de s'insérer -par frottement dans le tube (c).  firmly hold the swab (d) and insert it by friction into the tube (c). 4) Dispositif selon la revendication t caractérisé en ce que la forme et le diamètre du bouchon creux (-f), realise en plastique moulé, lui permet entre inséré fermement et soudé par ultra-violet à li extrémité inferieure du tube (c) et contenir un milieu de transport (g) pour micro-organismes. 4) Device according to claim t characterized in that the shape and the diameter of the hollow plug (-f), made of molded plastic, allows it between firmly inserted and welded by ultraviolet at the lower end of the tube (c) and contain a transport medium (g) for microorganisms. 5) Dispositif selon les revendications 1 et 4 caractérisé en ce que le bouchon creux (f) -est obture, après 5) Device according to claims 1 and 4 characterized in that the hollow plug (f) -is closed, after introduction du milieu (g), par un opercule (e) étanche et q-1e ledit opercule peut être réalisé soit en un film de plastique cassant soit en une feuille d'aluminium frangible. introduction of the medium (g), by a tight seal (e) and q-1e said seal can be made either in a brittle plastic film or in a frangible aluminum foil. 6) Dispositif selon la revendication t caractérisé en ce que la bague (b), réalisée en deux demi segments de plastique ou de carton, est positionee, calee et maintenue par 6) Device according to claim t characterized in that the ring (b), made of two half segments of plastic or cardboard, is positioned, wedged and held by une bandelette (h) de papier adhésif, entre le haut du tube(c) et l'épaulement inférieur du bouchon (a) de façon à empècher ou permettre, selon qu'elle est placée ou ovée, le contact entre l'écouvillon (d) et le milieu (g) a strip (h) of adhesive paper, between the top of the tube (c) and the lower shoulder of the stopper (a) so as to prevent or allow, as it is placed or ovate, the contact between the swab ( d) and the medium (g) 7) Dispositif selon les revendications 1, 4, 5 et 6 caractérisé en ce que le milieu (g) présent à l'intérieur du bouchon (f) peut Btre modifié à gré selon la nature et les exigences particulières des micro-organismes à prélever. 7) Device according to claims 1, 4, 5 and 6 characterized in that the medium (g) present inside the plug (f) can be modified as desired according to the nature and the specific requirements of the microorganisms to be sampled . 8) Dispositif selon les revendications 1, 2, 3, 4 et 6 caractérisé en ce que le tube (c) est réalisé en plastique par un procédé connu d'extrusion 8) Device according to claims 1, 2, 3, 4 and 6 characterized in that the tube (c) is made of plastic by a known extrusion process 9) Dispositif selon les revendications t, 3 et 6 caractérisé en ce que l'écouvillon (d) est réalisé en une boule de rayonne enroulée autour de l'extrémité d'une tige plastique. 9) Device according to claims t, 3 and 6 characterized in that the swab (d) is made of a rayon ball wound around the end of a plastic rod. 10) Dispositif selon l 8 une quelconque des revendications précédentes caractérisé en ce que le dispositif monté, prêt à l'emploi, peut être stérilisé par des moyens usuels faisant appel à des radiations  10) Device according to 8 any of the preceding claims characterized in that the mounted device, ready for use, can be sterilized by conventional means using radiation
FR8302167A 1983-02-09 1983-02-09 Device for preserving and transporting a bacterial sample Granted FR2540513A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR8302167A FR2540513A1 (en) 1983-02-09 1983-02-09 Device for preserving and transporting a bacterial sample

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8302167A FR2540513A1 (en) 1983-02-09 1983-02-09 Device for preserving and transporting a bacterial sample

Publications (2)

Publication Number Publication Date
FR2540513A1 true FR2540513A1 (en) 1984-08-10
FR2540513B1 FR2540513B1 (en) 1985-05-10

Family

ID=9285811

Family Applications (1)

Application Number Title Priority Date Filing Date
FR8302167A Granted FR2540513A1 (en) 1983-02-09 1983-02-09 Device for preserving and transporting a bacterial sample

Country Status (1)

Country Link
FR (1) FR2540513A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0308230A3 (en) * 1987-09-18 1989-12-13 EASTMAN KODAK COMPANY (a New Jersey corporation) Extraction device
WO2006131424A1 (en) * 2005-06-08 2006-12-14 Copan Innovation Limited Device for the withdrawal, collection and transport of biological specimens
EP1976635A4 (en) * 2006-01-13 2011-06-22 Bode Technology Group Inc APPARATUS AND METHOD FOR COLLECTING THE PROOF OF A PHYSICAL CONTACT

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB945190A (en) * 1961-10-16 1963-12-23 Charles Wilson Jinkens A container for storing specimens
US4175008A (en) * 1978-06-26 1979-11-20 Bio-Pharmaceutical Packaging Corp. Culture specimen collection and transport package
GB2028868A (en) * 1978-08-25 1980-03-12 Precision Dynamics Corp Culture collection and transport device
AU517787B2 (en) * 1976-11-09 1981-08-27 Hardie Trading Ltd. Culture or sample container

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB945190A (en) * 1961-10-16 1963-12-23 Charles Wilson Jinkens A container for storing specimens
AU517787B2 (en) * 1976-11-09 1981-08-27 Hardie Trading Ltd. Culture or sample container
US4175008A (en) * 1978-06-26 1979-11-20 Bio-Pharmaceutical Packaging Corp. Culture specimen collection and transport package
GB2028868A (en) * 1978-08-25 1980-03-12 Precision Dynamics Corp Culture collection and transport device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0308230A3 (en) * 1987-09-18 1989-12-13 EASTMAN KODAK COMPANY (a New Jersey corporation) Extraction device
WO2006131424A1 (en) * 2005-06-08 2006-12-14 Copan Innovation Limited Device for the withdrawal, collection and transport of biological specimens
EP1976635A4 (en) * 2006-01-13 2011-06-22 Bode Technology Group Inc APPARATUS AND METHOD FOR COLLECTING THE PROOF OF A PHYSICAL CONTACT

Also Published As

Publication number Publication date
FR2540513B1 (en) 1985-05-10

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