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FR2377626A1 - Electrophoresis appts. for fractionating materials on a gel - contains two buffer solns. electrically isolated in a container - Google Patents

Electrophoresis appts. for fractionating materials on a gel - contains two buffer solns. electrically isolated in a container

Info

Publication number
FR2377626A1
FR2377626A1 FR7701042A FR7701042A FR2377626A1 FR 2377626 A1 FR2377626 A1 FR 2377626A1 FR 7701042 A FR7701042 A FR 7701042A FR 7701042 A FR7701042 A FR 7701042A FR 2377626 A1 FR2377626 A1 FR 2377626A1
Authority
FR
France
Prior art keywords
container
gel
appts
electrophoresis
separated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7701042A
Other languages
French (fr)
Other versions
FR2377626B1 (en
Inventor
Jean Legault-Demare
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bpifrance Financement SA
Original Assignee
Agence National de Valorisation de la Recherche ANVAR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agence National de Valorisation de la Recherche ANVAR filed Critical Agence National de Valorisation de la Recherche ANVAR
Priority to FR7701042A priority Critical patent/FR2377626A1/en
Publication of FR2377626A1 publication Critical patent/FR2377626A1/en
Application granted granted Critical
Publication of FR2377626B1 publication Critical patent/FR2377626B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The electrophoresis is carried out on a vertical gel whch is in contact with two buffer solutions, one of which is anodic and the other cathodic, the two being electrically isolated. The two buffer solutions are contained with one container, separated by a layer of a third liq. which is non-miscible, and non-reactive with the other two and acts as a liq. insulator. Various arrangements of the apparatus are possible but in each case a section of the container is separated from the rest in such a way that the lower buffer solution may rise up the separated section to reach virtually the same height as the top of the upper buffer solution thus allowing the electrode to be placed in the lower buffer solution without passing through the two solution above it and also making a route for gases produced to escape. Appts. may be used to fractionate proteins, nucleic acids and in general any substance which needs to be fractionated by electrophoresis of a gel. The appts. is simple and may be used with varying size plates. It can be used for the recovery of the fractionated substances by electrophoretic elution giving a good yield.
FR7701042A 1977-01-14 1977-01-14 Electrophoresis appts. for fractionating materials on a gel - contains two buffer solns. electrically isolated in a container Granted FR2377626A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7701042A FR2377626A1 (en) 1977-01-14 1977-01-14 Electrophoresis appts. for fractionating materials on a gel - contains two buffer solns. electrically isolated in a container

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7701042A FR2377626A1 (en) 1977-01-14 1977-01-14 Electrophoresis appts. for fractionating materials on a gel - contains two buffer solns. electrically isolated in a container

Publications (2)

Publication Number Publication Date
FR2377626A1 true FR2377626A1 (en) 1978-08-11
FR2377626B1 FR2377626B1 (en) 1980-03-28

Family

ID=9185504

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7701042A Granted FR2377626A1 (en) 1977-01-14 1977-01-14 Electrophoresis appts. for fractionating materials on a gel - contains two buffer solns. electrically isolated in a container

Country Status (1)

Country Link
FR (1) FR2377626A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5073246A (en) * 1990-05-16 1991-12-17 Bio-Rad Laboratories, Inc. Slab electrophoresis system with improved sample wells and cooling mechanism

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5073246A (en) * 1990-05-16 1991-12-17 Bio-Rad Laboratories, Inc. Slab electrophoresis system with improved sample wells and cooling mechanism

Also Published As

Publication number Publication date
FR2377626B1 (en) 1980-03-28

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