SU552373A1 - Electrophoretic Elution Apparatus - Google Patents
Electrophoretic Elution ApparatusInfo
- Publication number
- SU552373A1 SU552373A1 SU2080651A SU2080651A SU552373A1 SU 552373 A1 SU552373 A1 SU 552373A1 SU 2080651 A SU2080651 A SU 2080651A SU 2080651 A SU2080651 A SU 2080651A SU 552373 A1 SU552373 A1 SU 552373A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- elution apparatus
- electrophoretic elution
- cell
- compartment
- fractions
- Prior art date
Links
Landscapes
- Peptides Or Proteins (AREA)
Description
нее отделение 7 снизу ограничено полупроницаемой мембраной 8, котора герметично прижата к телу чейки 4 уплотнительным кольцом 9. Дл слива элюированных фракций в нижнем отделении 7 чейки 4 установлен штуцер 10.its compartment 7 is bounded below by a semipermeable membrane 8, which is hermetically pressed against the body of the cell 4 with a sealing ring 9. A fitting 10 is installed to drain the eluted fractions in the lower compartment 7 of cell 4.
Аппарат работает следующим образом.The device works as follows.
Вырезанные из блока полиакриламидного гел отдельные -белковые фракции размельчаютс п помещаютс в верхнее отделение 6 чейки 4. Верхн электродна секци 1 устанавливаетс в нижней 2, и обе секции заливаютс электродным буферным раствором так, чтобы уровень буфера в нижней секции 2 был выше полупроницаемой мембраны в. При подаче на электроды 3 аппарата выпр мленного напр жени белковые компоненты мигрируют из гел в нижнее отделение 7 чейки 4, скапливаютс там и вывод тс по истечении определенного времени через штуцер 10.The individual protein fractions cut from the polyacrylamide gel block are crushed and placed in the upper compartment 6 of cell 4. The upper electrode section 1 is installed in the lower 2, and both sections are filled with electrode buffer solution so that the level of the buffer in the lower section 2 is above the semipermeable membrane in. When a straightened voltage is applied to the electrodes 3 of the apparatus, protein components migrate from the gel to the lower compartment 7 of cell 4, accumulate there and are released after a certain time through fitting 10.
Така конструкци аппарата, благодар наличию верхнего отделени чейки, отделенного сеткой, позвол ет работать с измельченным гелем, обеспечива получение отдельных фракций с выходом до 92-95% за минимальное врем . Наличие нижнего отделени меньшего объема позвол ет сконцентрировать выделение фракции в значительно меньшем объеме, чем они содержались в геле. Простота и разборность конструкции облегчают работу на аппарате и исключают возможность загр знени выдел емы.х фракций другими компонентами.This design of the apparatus, due to the presence of the upper compartment of the cell, separated by a mesh, allows working with the crushed gel, providing separate fractions with a yield of up to 92-95% in the shortest time. The presence of a lower compartment of smaller volume allows the fraction of the fraction to be concentrated in a much smaller volume than they were contained in the gel. The simplicity and disassembly of the design facilitates the work on the apparatus and eliminates the possibility of contamination of the excreted fractions by other components.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU2080651A SU552373A1 (en) | 1974-12-03 | 1974-12-03 | Electrophoretic Elution Apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU2080651A SU552373A1 (en) | 1974-12-03 | 1974-12-03 | Electrophoretic Elution Apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU552373A1 true SU552373A1 (en) | 1977-03-30 |
Family
ID=20602428
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU2080651A SU552373A1 (en) | 1974-12-03 | 1974-12-03 | Electrophoretic Elution Apparatus |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU552373A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4576702A (en) * | 1984-11-05 | 1986-03-18 | International Biotechnologies, Inc. | Analytical electroelution device |
| US4576703A (en) * | 1984-11-05 | 1986-03-18 | International Biotechnologies, Inc. | Preparatory electroelution device |
| US4725348A (en) * | 1984-05-09 | 1988-02-16 | Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. | Device for the elution of electrically-charged macromolecules |
-
1974
- 1974-12-03 SU SU2080651A patent/SU552373A1/en active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4725348A (en) * | 1984-05-09 | 1988-02-16 | Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. | Device for the elution of electrically-charged macromolecules |
| US4576702A (en) * | 1984-11-05 | 1986-03-18 | International Biotechnologies, Inc. | Analytical electroelution device |
| US4576703A (en) * | 1984-11-05 | 1986-03-18 | International Biotechnologies, Inc. | Preparatory electroelution device |
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