[go: up one dir, main page]

FR15C0055I2 - IN VITRO RECONSTRUCTED HUMAN CORNEAL EPITHELIUM SHEETS AND METHOD FOR PRODUCING THEM - Google Patents

IN VITRO RECONSTRUCTED HUMAN CORNEAL EPITHELIUM SHEETS AND METHOD FOR PRODUCING THEM

Info

Publication number
FR15C0055I2
FR15C0055I2 FR15C0055C FR15C0055C FR15C0055I2 FR 15C0055 I2 FR15C0055 I2 FR 15C0055I2 FR 15C0055 C FR15C0055 C FR 15C0055C FR 15C0055 C FR15C0055 C FR 15C0055C FR 15C0055 I2 FR15C0055 I2 FR 15C0055I2
Authority
FR
France
Prior art keywords
producing
corneal epithelium
human corneal
reconstructed human
vitro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR15C0055C
Other languages
French (fr)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HOLOSTEM TERAPIE AVANZATE Srl
Original Assignee
HOLOSTEM TERAPIE AVANZATE Srl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HOLOSTEM TERAPIE AVANZATE Srl filed Critical HOLOSTEM TERAPIE AVANZATE Srl
Publication of FR15C0055I1 publication Critical patent/FR15C0055I1/fr
Application granted granted Critical
Publication of FR15C0055I2 publication Critical patent/FR15C0055I2/en
Active legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/56—Fibrin; Thrombin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Prostheses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of reconstructing laminae of human epithelium corneae in vitro to be used in grafts from cultures of limbar stem cells as well as a method of selecting and transferring such cells to fibrin substrate.
FR15C0055C 2001-08-03 2015-07-29 IN VITRO RECONSTRUCTED HUMAN CORNEAL EPITHELIUM SHEETS AND METHOD FOR PRODUCING THEM Active FR15C0055I2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT2001RM000476A ITRM20010476A1 (en) 2001-08-03 2001-08-03 HUMAN CORNEAL EPITHELIUM SHEETS RECONSTRUCTED AND METHOD FOR THEIR OBTAINING.
PCT/IT2001/000603 WO2003012083A1 (en) 2001-08-03 2001-11-28 In vitro reconstituted sheets of human corneal epithelium and method of producing the same

Publications (2)

Publication Number Publication Date
FR15C0055I1 FR15C0055I1 (en) 2015-09-11
FR15C0055I2 true FR15C0055I2 (en) 2016-06-24

Family

ID=11455714

Family Applications (1)

Application Number Title Priority Date Filing Date
FR15C0055C Active FR15C0055I2 (en) 2001-08-03 2015-07-29 IN VITRO RECONSTRUCTED HUMAN CORNEAL EPITHELIUM SHEETS AND METHOD FOR PRODUCING THEM

Country Status (12)

Country Link
US (1) US6610538B2 (en)
EP (1) EP1451302B1 (en)
AT (1) ATE397058T1 (en)
CY (1) CY1108257T1 (en)
DE (1) DE60134280D1 (en)
DK (1) DK1451302T3 (en)
ES (1) ES2307570T3 (en)
FR (1) FR15C0055I2 (en)
IT (1) ITRM20010476A1 (en)
NL (1) NL300754I2 (en)
PT (1) PT1451302E (en)
WO (1) WO2003012083A1 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050186672A1 (en) * 2004-01-27 2005-08-25 Reliance Life Sciences Pvt. Ltd. Tissue system with undifferentiated stem cells derived from corneal limbus
US8187875B2 (en) * 2004-02-26 2012-05-29 Reliance Life Sciences Pvt. Ltd. Dopaminergic neurons derived from corneal limbus, methods of isolation and uses thereof
BRPI0506536C1 (en) * 2004-02-26 2007-05-22 Reliance Life Sciences Pvt Ltd corneal limbus-derived dopaminergic neurons, isolation methods and uses
US20060216821A1 (en) * 2004-02-26 2006-09-28 Reliance Life Sciences Pvt. Ltd. Pluripotent embryonic-like stem cells derived from corneal limbus, methods of isolation and uses thereof
ITRM20040273A1 (en) * 2004-06-03 2004-09-03 Farmigea Spa METHOD FOR ISOLATION AND EX VIVO EXPANSION OF HUMAN CORNEAL STEM CELLS AND THEIR RELATED USES.
US8338175B2 (en) * 2006-02-24 2012-12-25 Reliance Life Sciences Pvt. Ltd. Conjunctival tissue system
WO2011040888A1 (en) * 2009-10-01 2011-04-07 Singapore Health Services Pte Ltd A biodegradable composition or combination and uses thereof
RU2505303C2 (en) * 2011-09-13 2014-01-27 Общество с ограниченной ответственностью "Офтальмологические биотехнологии" Method for preparing corneal biotransplant
FR2993282B1 (en) 2012-07-13 2017-11-10 Expanscience Lab METHOD FOR IDENTIFYING MOLECULAR MARKERS OF CHILD'S SKIN
FR3011008B1 (en) 2013-09-24 2017-12-29 Expanscience Lab METHODS OF EVALUATING DELETER EFFECTS OF UV ON CHILD'S SKIN
FR3016373B1 (en) 2014-01-10 2018-01-19 Laboratoires Expanscience MAMMELON SKIN MODEL RECONSTITUTES
FR3019186B1 (en) 2014-03-31 2019-06-07 Laboratoires Expanscience METHODS OF EVALUATING THE DELETE EFFECTS OF URINE ON CHILDREN'S SKIN
FR3045669B1 (en) 2015-12-16 2019-04-05 Laboratoires Expanscience METHODS OF EVALUATING THE EFFECTS OF DEHYDRATION ON CHILDREN'S SKIN
FR3053053B1 (en) 2016-06-23 2018-07-13 Laboratoires Expanscience MODELS OF ATOPIC JUVENILE DERMATITIS
FR3068045B1 (en) 2017-06-22 2021-06-04 Expanscience Lab RECONSTITUTED SENSITIVE SKIN MODELS
EP3867357B1 (en) * 2018-10-19 2024-09-25 Regents of the University of Minnesota Methods of isolating and using descemet's membrane and compositions including isolated descemet's membrane
FR3133198A1 (en) 2022-03-04 2023-09-08 Pierre Fabre Dermo-Cosmetique RECONSTITUTED SKIN MODEL
FR3138149A1 (en) 2022-07-25 2024-01-26 Pierre Fabre Dermo-Cosmetique IN VITRO EVALUATION METHOD OF THE PHOTOPROTECTIVE ACTIVITY OF AN ACTIVE

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1255657B (en) 1992-05-29 1995-11-09 DIFFERENTIATED CONJUNCTIVAL MUCOSA FABRIC CULTURES, PROCEDURES FOR THEIR PREPARATION AND SUPPORTS FOR THEIR USE.
US5827641A (en) 1992-11-13 1998-10-27 Parenteau; Nancy L. In vitro cornea equivalent model
CA2227827A1 (en) 1998-01-23 1999-07-23 Unknown In vitro artificial cornea and sclera

Also Published As

Publication number Publication date
EP1451302A1 (en) 2004-09-01
PT1451302E (en) 2008-09-08
DK1451302T3 (en) 2008-09-08
US6610538B2 (en) 2003-08-26
EP1451302B1 (en) 2008-05-28
WO2003012083A1 (en) 2003-02-13
DE60134280D1 (en) 2008-07-10
CY1108257T1 (en) 2014-02-12
FR15C0055I1 (en) 2015-09-11
ITRM20010476A0 (en) 2001-08-03
NL300754I2 (en) 2016-09-01
ES2307570T3 (en) 2008-12-01
ATE397058T1 (en) 2008-06-15
US20030027333A1 (en) 2003-02-06
ITRM20010476A1 (en) 2003-02-03

Similar Documents

Publication Publication Date Title
FR15C0055I2 (en) IN VITRO RECONSTRUCTED HUMAN CORNEAL EPITHELIUM SHEETS AND METHOD FOR PRODUCING THEM
TR200401091T4 (en) Cell culture medium containing growth factors and L-glutamine
EP1165830A4 (en) STEM CELLS AND ARRAYS DERIVED FROM FATTY TISSUES
CY1107771T1 (en) METHOD FOR PRODUCING WHOLESALE CARPETS FROM IN VITRO CROPPED CROPS
IL144446A0 (en) Plasma protein matrices and methods for their preparation
CA2407439A1 (en) Individual venous valve prosthesis
AU2002236795A1 (en) Compositions and method of tissue superoxygenation
MXPA03001914A (en) Electroprocessed fibrin-based matrices and tissues.
TW200513535A (en) Enhanced production of lipids containing polyenoic fatty acids by very high density cultures of eukaryotic microbe in fermentors
TR200201144T2 (en) Substituted indoles for modulation of NFKB activity
MXPA04001334A (en) Materials and methods to promote repair of nerve tissue.
AU2003304250A1 (en) Method of isolating cells from umbilical cord
PT1318198E (en) PROCESS FOR PRODUCING A RECOMBINANT POLYPEPTIDE INVOLVING THE ADDITION OF A QUIMOTRYPSIN INHIBITOR TO THE CELLULAR CULTURE ENVIRONMENT
DE50005037D1 (en) METHOD FOR PRODUCING UNDERWEAR PIECES BY MEANS OF ADHESIVE CONNECTION
AU2002325782A1 (en) Pharmaceutical composition and method of treatment of human malignancies with arginine deprivation
DE60110047D1 (en) TOOTH PROCESSOR CELL AND METHOD FOR THE PRODUCTION THEREOF
EP1334982A3 (en) Solubilized complement receptor type 1 in crystal form
IL178513A (en) Process for the generation of epithelial cells in culture and a cultivation device for making the same
HUP0101913A2 (en) Immortalised cell lines derived from normal human skin tissues
TW200407133A (en) Novel PPARα and PPARγ agonists
DE60226896D1 (en) PREPARATION OF F (AB ') 2 FRAGMENTS IN TISSUE CELL
WO2004043373A3 (en) Precursor n-acetylgalactosamine-4-sulfatase, methods of treatment using said enzyme and methods for producing and purifying said enzyme
DE60322064D1 (en) 4,4'-DITHIOBIS- (3-AMINOBUTANE-1-SULPHONATE) DERIVATIVES AND COMPOSITIONS CONTAINING THEM
NO20032977L (en) Use of biological material containing three-dimensional dissertation structures of hyaluronic acid derivatives for the preparation of implants for arthroscopy.
DE60334372D1 (en) PROCESS FOR PRODUCING FABRIC CONSTRUCTS