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BRPI0506536C1 - corneal limbus-derived dopaminergic neurons, isolation methods and uses - Google Patents

corneal limbus-derived dopaminergic neurons, isolation methods and uses

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Publication number
BRPI0506536C1
BRPI0506536C1 BRC10506536-4A BRPI0506536A BRPI0506536C1 BR PI0506536 C1 BRPI0506536 C1 BR PI0506536C1 BR PI0506536 A BRPI0506536 A BR PI0506536A BR PI0506536 C1 BRPI0506536 C1 BR PI0506536C1
Authority
BR
Brazil
Prior art keywords
elscs
dopaminergic neurons
cells
generation
isolation methods
Prior art date
Application number
BRC10506536-4A
Other languages
Portuguese (pt)
Inventor
Geeta Ravindran
Harinarayana S Rao
Original Assignee
Reliance Life Sciences Pvt Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reliance Life Sciences Pvt Ltd filed Critical Reliance Life Sciences Pvt Ltd
Publication of BRPI0506536A publication Critical patent/BRPI0506536A/en
Publication of BRPI0506536C1 publication Critical patent/BRPI0506536C1/en

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    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
    • C12N2502/085—Coculture with; Conditioned medium produced by cells of the nervous system eye cells
    • C—CHEMISTRY; METALLURGY
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Neurology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Neurosurgery (AREA)
  • Rheumatology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Cardiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Psychology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)

Abstract

NEURÈNIOS DOPAMINéRGICOS DERIVADOS DO LIMBUS CORNEAL, MéTODOS DE ISOLAMENTO E USOS DOS MESMOS, compreendendo a geração de células neurais e de neurónios a partir de células-tronco do tipo embriónico (ELSCs) pluripotentes de mamífero isoladas do tecido límbico corneal, um tecido não embriónico; mais particularmente, a geração de células neuroprogenitoras e de neurónios dopaminérgicos diferenciados de ELSCs; sendo que os métodos descritos demonstram o potencial das ELSCs como uma ferramenta terapêutica e podem fornecer novas alternativas terapêuticas para várias doenças afecções e lesões, como também as células neuroprogenitoras geradas a partir das ELSCs isoladas do tecido límbico corneal foram suficientes para aliviar as anomalias motoras em um modelo de rato de mal de PARKINSON por pelo menos seis meses.DOPAMINERGIC NEURONS DERIVED FROM CORNAL LIMBUS, INSULATION METHODS AND USES OF THE SAME, comprising the generation of neural cells and neurons from pluripotent mammalian embryonic stem cells (ELSCs), a non-embryonic tissue; more particularly the generation of neuroprogenitor cells and differentiated dopaminergic neurons from ELSCs; The described methods demonstrate the potential of ELSCs as a therapeutic tool and may provide new therapeutic alternatives for various diseases, disorders and injuries, as well as neuroprogenitor cells generated from ELSCs isolated from corneal limbic tissue were sufficient to alleviate motor abnormalities in a PARKINSON evil mouse model for at least six months.

BRC10506536-4A 2004-02-26 2005-02-23 corneal limbus-derived dopaminergic neurons, isolation methods and uses BRPI0506536C1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IN240MU2004 2004-02-26
US62147604P 2004-10-22 2004-10-22
PCT/IB2005/000516 WO2005084119A2 (en) 2004-02-26 2005-02-23 Pluripotent embryonic-like stem cells derived from corneal limbus, methods of isolation and uses thereof

Publications (2)

Publication Number Publication Date
BRPI0506536A BRPI0506536A (en) 2007-05-15
BRPI0506536C1 true BRPI0506536C1 (en) 2007-05-22

Family

ID=34921522

Family Applications (1)

Application Number Title Priority Date Filing Date
BRC10506536-4A BRPI0506536C1 (en) 2004-02-26 2005-02-23 corneal limbus-derived dopaminergic neurons, isolation methods and uses

Country Status (6)

Country Link
EP (1) EP1758984A2 (en)
KR (3) KR100840979B1 (en)
AU (1) AU2005220066B2 (en)
BR (1) BRPI0506536C1 (en)
IL (2) IL177671A0 (en)
WO (1) WO2005084119A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050186672A1 (en) * 2004-01-27 2005-08-25 Reliance Life Sciences Pvt. Ltd. Tissue system with undifferentiated stem cells derived from corneal limbus
MY165114A (en) * 2006-09-01 2018-02-28 Stempeutics Res Private Limited Self-renewing master adult pluripotent stem cells
CN103068972B (en) * 2010-02-22 2016-09-21 皮埃尔-玛丽-居里大学(巴黎第六大学) For hematopoietic lineage cell growth and the cell culture medium of differentiation
KR101323650B1 (en) * 2011-05-04 2013-11-05 연세대학교 산학협력단 Methods for Depleting Undifferentiated Pluripotent Stem Cells
AU2016227607B2 (en) 2015-03-04 2021-09-30 Mesoblast International Sarl Cell culture method for mesenchymal stem cells
KR102038503B1 (en) * 2015-08-12 2019-11-26 (주) 넥셀 Method for differentiation of hepatocyte
KR101645901B1 (en) 2015-12-31 2016-08-04 가톨릭대학교 산학협력단 Method for culturing limbal stem cell using amniotic membrane slide scaffold
KR101789917B1 (en) 2016-03-29 2017-10-26 가톨릭대학교 산학협력단 Method for increasing the proliferation and stemness of limbal stem cells
CN107345897A (en) * 2016-05-04 2017-11-14 上海米洋杨生物科技有限公司 A kind of screening technique of retinal photoreceptor cells specific surfaces albumen
WO2018094209A1 (en) * 2016-11-18 2018-05-24 Rita Perlingeiro Surface markers for the isolation of myogenic stem/progenitor cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5703047A (en) 1992-09-21 1997-12-30 Board Of Regents, The University Of Texas System Methods and treatments for corneal healing with growth factors
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US20030161817A1 (en) * 2001-03-28 2003-08-28 Young Henry E. Pluripotent embryonic-like stem cells, compositions, methods and uses thereof
ITRM20010476A1 (en) * 2001-08-03 2003-02-03 Idi Irccs HUMAN CORNEAL EPITHELIUM SHEETS RECONSTRUCTED AND METHOD FOR THEIR OBTAINING.
US7422736B2 (en) * 2002-07-26 2008-09-09 Food Industry Research And Development Institute Somatic pluripotent cells

Also Published As

Publication number Publication date
BRPI0506536A (en) 2007-05-15
EP1758984A2 (en) 2007-03-07
KR20060114372A (en) 2006-11-06
KR100840979B1 (en) 2008-06-24
KR20080036636A (en) 2008-04-28
WO2005084119A3 (en) 2010-06-17
WO2005084119A2 (en) 2005-09-15
IL177672A (en) 2011-03-31
IL177672A0 (en) 2008-03-20
KR20090021229A (en) 2009-02-27
AU2005220066A1 (en) 2005-09-15
AU2005220066B2 (en) 2010-01-21
KR100931887B1 (en) 2009-12-15
IL177671A0 (en) 2008-03-20

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