FI122936B - Ksylanaser med förbättrad termofilitet och alkalifilitet - Google Patents
Ksylanaser med förbättrad termofilitet och alkalifilitet Download PDFInfo
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- FI122936B FI122936B FI20031094A FI20031094A FI122936B FI 122936 B FI122936 B FI 122936B FI 20031094 A FI20031094 A FI 20031094A FI 20031094 A FI20031094 A FI 20031094A FI 122936 B FI122936 B FI 122936B
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- xylanase
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- xylanases
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- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
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- TYTUEXPNTQGSDO-UHFFFAOYSA-N hydrazine 2-hydroxybenzoic acid Chemical compound NN.OC1=C(C(=O)O)C=CC=C1 TYTUEXPNTQGSDO-UHFFFAOYSA-N 0.000 description 1
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- KUIXZSYWBHSYCN-UHFFFAOYSA-L remazol brilliant blue r Chemical compound [Na+].[Na+].C1=C(S([O-])(=O)=O)C(N)=C2C(=O)C3=CC=CC=C3C(=O)C2=C1NC1=CC=CC(S(=O)(=O)CCOS([O-])(=O)=O)=C1 KUIXZSYWBHSYCN-UHFFFAOYSA-L 0.000 description 1
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
- C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
- D21C9/10—Bleaching ; Apparatus therefor
- D21C9/1026—Other features in bleaching processes
- D21C9/1036—Use of compounds accelerating or improving the efficiency of the processes
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Paper (AREA)
Claims (13)
1. Ett modifierat xylanas Μη familj 11, som innefattar ett substituerat arginin (Arg) vid positionen 144, och frän 0 till 11 andra substituerade aminosyror, varvid nämnda position bestäms frän linjeringen mellan amino syrasekvenser av 5 nämnda modifierade familj 11 och sekvens nummer 16, väri nämnda modifierade familj 11 xylanas uppvisar förbättrad termofilicitet i jämforelse med motsvarande familj 11 nativa xylanas, frän vilket nämnda modifierade familj 11 xylanas är här-lett.
2. Modifierat familj 11 xylanas enligt krav 1, väri det modifierade xylanaset 10 uppvisar förbättrad termofilicitet i jämförelse med motsvarande familj 11 nativa xylanas, frän vilket nämnda modifierade familj 11 xylanas är härlett.
3. Modifierat familj 11 xylanas enligt krav 1 eller 2, som innefattar en substituer-ad asparaginsyra (Asp) vid position 11.
4. Modifierat familj 11 xylanas enligt nägot av kraven 1-3, som innefattar ett 15 substituerat glycin (Gly) vid position 116.
5. Modifierat familj 11 xylanas enligt nägot av kraven 1-3, som innefattar ett substituerat cystein (Cys) vid position 118.
6. Modifierat familj 11 xylanas enligt nägot av kraven 1-5, väri nämnda familj 11 xylanas är ett modifierat Trichoderma reesei xylanas.
7. Modifierat familj 11 xylanas enligt nägot av kraven 1-6, väri nämnda modifi erade Trichoderma reesei xylanas är ett modifierat Trichoderma reesei xylanas II. C\J c3
8. Modifierat familj 11 xylanas enligt nägot av kraven 1-7, vilket vidare innefat- g tar substituerade histidiner (His) vid positionerna 10 och 105, ett substituerat me- g tionin (Met) vid position 27, ett substituerat leucin (Leu) vid position 29, substituer- x 25 ade alaniner (Ala) vid positionerna 75 och 125, ett substituerat glutaminsyra (Glu) tr vid position 129 och ett substituerat arginin (Arg) vid position 161. CD
° 9. Modifierat familj 11 xylanas som väljs frän gruppen, som bestär av: CO o <N TrX-HML-7 5 A105H-125 A129E-144R TrX-HML-75 A105H-125 A129E-144R161R 30 TrX-HML-75 A105H-116G-125A129E-144R TrX-HML-75 A105H-118C-125 A129E-144R TrX-H-11D-ML-75 A105H-125 A129E-144R161R TrX-H-11 D-ML-75 A105H-116G-125A129E-144R161R TrX-H-11 D-ML-75 A105H-118C-125 A129E-144R161R 5 TrX-H-11 D-ML-75 A105H-116G118C-125A129E-144R161R.
10. Modifierat familj 11 xylanas enligt nägot av kraven 1 - 9, väri nämnda modifi-erade familj 11 xylanas kännetecknas av att den har en maximal effektiv temperatur (MET) mellan omkring 69 °C och omkring 84 °C.
11. Modifierat familj 11 xylanas enligt nägot av kraven 1 - 9, väri nämnda modi-10 fierade familj 11 xylanas kännetecknas av att den har ett maximalt effektivt pH (MEP) mellan omkring pH 5,8 och omkring pH 8,4.
12. Ett industriellt förfarande, i vilket ett xylanassubstrat hydrolyseras med ett modifierat familj 11 xylanas enligt nägot av kraven 1-11.
13. Industriellt förfarande enligt krav 12, väri nämnda xylanassubstrat som skall 15 hydrolyseras är i fibermassan. CM δ CM LO O oo o X cr CL CD O δ o o CM
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US99087401 | 2001-11-21 | ||
| US09/990,874 US7510860B1 (en) | 2001-11-21 | 2001-11-21 | Xylanases with enhanced thermophilicity and alkalophilicity |
| CA0201758 | 2002-11-20 | ||
| PCT/CA2002/001758 WO2003046169A2 (en) | 2001-11-21 | 2002-11-20 | Xylanases with enhanced thermophilicity and alkalophilicity |
Publications (2)
| Publication Number | Publication Date |
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| FI20031094L FI20031094L (sv) | 2003-09-18 |
| FI122936B true FI122936B (sv) | 2012-09-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| FI20031094A FI122936B (sv) | 2001-11-21 | 2003-07-21 | Ksylanaser med förbättrad termofilitet och alkalifilitet |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7510860B1 (sv) |
| CN (1) | CN1697877B (sv) |
| AU (1) | AU2002342464A1 (sv) |
| CA (1) | CA2435527C (sv) |
| FI (1) | FI122936B (sv) |
| SE (1) | SE526842C2 (sv) |
| WO (1) | WO2003046169A2 (sv) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR0111316A (pt) * | 2000-05-31 | 2003-12-16 | Ca Nat Research Council | Xilanase modificada e uso da mesma |
| BRPI0509168A (pt) | 2004-03-25 | 2007-09-11 | Iogen Bio Products Corp | xilanases modificadas que exibem expressão melhorada |
| WO2007115391A1 (en) * | 2006-04-12 | 2007-10-18 | National Research Council Of Cananda | Modification of xylanases to increase thermophilicity, thermostability and alkalophilicity |
| EP2784161B1 (en) | 2011-11-25 | 2018-06-20 | Mitsui Chemicals, Inc. | Mutant xylanase, manufacturing method and use therefor, and method for manufacturing saccharified lignocellulose |
| GB201401648D0 (en) | 2014-01-31 | 2014-03-19 | Dupont Nutrition Biosci Aps | Protein |
| GB201401699D0 (en) * | 2014-01-31 | 2014-03-19 | Dupont Nutrition Biosci Aps | Protein |
| BR112016027884B1 (pt) | 2014-05-30 | 2023-05-02 | Novozymes A/S | Variante de uma xilanase da família 11 de gh genitora, polinucleotídeo, construção de ácido nucleico, vetor de expressão, célula hospedeira, e, métodos de produção de uma variante de xilanase da família 11 de gh e de degradação de um material contendo xilana |
| CN107105679A (zh) | 2014-12-29 | 2017-08-29 | 洲际大品牌有限责任公司 | 酶促麸和胚芽风味以及质构改进 |
| CN109402091B (zh) * | 2017-08-18 | 2022-02-11 | 潍坊康地恩生物科技有限公司 | 木聚糖酶突变体 |
| JP7135334B2 (ja) * | 2018-02-22 | 2022-09-13 | 株式会社豊田中央研究所 | キシラナーゼ及びその利用 |
| CN109750016B (zh) * | 2019-03-27 | 2023-04-28 | 云南师范大学 | 热稳性提高的木聚糖酶突变体及其制备方法和应用 |
| KR102677156B1 (ko) * | 2021-08-06 | 2024-06-20 | 씨제이제일제당 주식회사 | 자일라나제 활성을 갖는 변이형 폴리펩티드 |
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| JP2573854B2 (ja) | 1987-12-12 | 1997-01-22 | 日興バイオ技研株式会社 | 超精密装置の超精密洗浄方法 |
| GB9018426D0 (en) | 1990-08-22 | 1990-10-03 | Sandoz Ltd | Improvements in or relating to novel compounds |
| AU671710B2 (en) * | 1992-12-24 | 1996-09-05 | Dsm N.V. | Cloning And Expression Of Xylanase B |
| US5405769A (en) | 1993-04-08 | 1995-04-11 | National Research Council Of Canada | Construction of thermostable mutants of a low molecular mass xylanase |
| WO1995009909A1 (en) * | 1993-10-04 | 1995-04-13 | Novo Nordisk A/S | An enzyme preparation comprising a modified enzyme |
| WO1995012668A1 (en) | 1993-11-05 | 1995-05-11 | Cornell Research Foundation, Inc. | Thermostable xylanase from a thermomonospora fusca gene |
| ES2169219T5 (es) * | 1995-01-26 | 2009-09-01 | Novozymes A/S | Aditivos alimenticios para animales que comprenden xilanasa. |
| US6228629B1 (en) * | 1995-12-18 | 2001-05-08 | Röhn Enzyme Finland OY | Xylanases, genes encoding them, and uses thereof |
| US5759840A (en) | 1996-09-09 | 1998-06-02 | National Research Council Of Canada | Modification of xylanase to improve thermophilicity, alkalophilicity and thermostability |
| WO2000029587A1 (en) | 1998-11-16 | 2000-05-25 | National Research Council Of Canada | Thermostable xylanases |
| EP2295558B1 (en) * | 2000-03-08 | 2017-11-01 | DuPont Nutrition Biosciences ApS | Xylanase variants |
| BR0111316A (pt) * | 2000-05-31 | 2003-12-16 | Ca Nat Research Council | Xilanase modificada e uso da mesma |
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2001
- 2001-11-21 US US09/990,874 patent/US7510860B1/en not_active Expired - Fee Related
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2002
- 2002-11-20 CN CN02823195.3A patent/CN1697877B/zh not_active Expired - Fee Related
- 2002-11-20 WO PCT/CA2002/001758 patent/WO2003046169A2/en not_active Application Discontinuation
- 2002-11-20 AU AU2002342464A patent/AU2002342464A1/en not_active Abandoned
- 2002-11-20 CA CA2435527A patent/CA2435527C/en not_active Expired - Fee Related
-
2003
- 2003-07-18 SE SE0302103A patent/SE526842C2/sv not_active IP Right Cessation
- 2003-07-21 FI FI20031094A patent/FI122936B/sv active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| US7510860B1 (en) | 2009-03-31 |
| SE0302103D0 (sv) | 2003-07-18 |
| WO2003046169A3 (en) | 2004-04-15 |
| SE526842C2 (sv) | 2005-11-08 |
| CA2435527C (en) | 2014-01-21 |
| CA2435527A1 (en) | 2003-06-05 |
| WO2003046169A2 (en) | 2003-06-05 |
| FI20031094L (sv) | 2003-09-18 |
| CN1697877B (zh) | 2011-05-11 |
| SE0302103L (sv) | 2003-09-19 |
| CN1697877A (zh) | 2005-11-16 |
| US20090075330A1 (en) | 2009-03-19 |
| AU2002342464A1 (en) | 2003-06-10 |
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