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ES8403158A1 - Un metodo para crear un microorganismo capaz de producir unaproteina predeterminada por medio de expresion. - Google Patents

Un metodo para crear un microorganismo capaz de producir unaproteina predeterminada por medio de expresion.

Info

Publication number
ES8403158A1
ES8403158A1 ES519234A ES519234A ES8403158A1 ES 8403158 A1 ES8403158 A1 ES 8403158A1 ES 519234 A ES519234 A ES 519234A ES 519234 A ES519234 A ES 519234A ES 8403158 A1 ES8403158 A1 ES 8403158A1
Authority
ES
Spain
Prior art keywords
heterologous gene
subtilis
microorganisms
creation
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES519234A
Other languages
English (en)
Other versions
ES519234A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp filed Critical Cetus Corp
Publication of ES8403158A1 publication Critical patent/ES8403158A1/es
Publication of ES519234A0 publication Critical patent/ES519234A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

METODO PARA CREAR UN MICROORGANISMO CAPAZ DE PRODUCIR UNA PROTEINA PREDETERMINADA POR MEDIO DE EXPRESION.CONSISTE EN FORMAR UN PLASMIDO CAPAZ DE REPLICACION EN B. SUTILIS O CAPAZ DE INTEGRACION EN EL CROMOSOMA BACTERIANO DE B. SUBTILIS, EL CUAL TIENE UN GEN HETEROLOGO QUE CODIFICA UNA PROTEINA PREDETERMINADA CAPAZ DE EXPRESION EN B. SUBTILIS. DICHO GEN HETEROLOGO INCLUYE UNA SECUENCIA DE CODON DE INICIACION A LA TRADUCCION Y ESTA BAJO EL CONTROL DE SEÑALES OPERABLES REGULADORAS DE LA TRANSCRIPCION Y LA TRADUCCION QUE INCLUYEN OPERADOR, PROMOTOR Y SECUENCIAS DEL SITIODE UNION AL RIBOSOMA, SIENDO DICHAS SEÑALES OPERABLES REGULADORAS DE LA TRANSCRIPCION Y LA TRADUCCION ORIGINARIAS DE UNA FUENTE DISTINTA DE DICHO GEN HETEROLOGO.
ES519234A 1981-04-20 1983-01-25 Un metodo para crear un microorganismo capaz de producir unaproteina predeterminada por medio de expresion. Granted ES519234A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US25580481A 1981-04-20 1981-04-20

Publications (2)

Publication Number Publication Date
ES8403158A1 true ES8403158A1 (es) 1984-03-01
ES519234A0 ES519234A0 (es) 1984-03-01

Family

ID=22969947

Family Applications (2)

Application Number Title Priority Date Filing Date
ES511510A Granted ES511510A0 (es) 1981-04-20 1982-04-19 Un metodo para producir una proteina predeterminada por cul tivo de bacterias b subtilis transformadas.
ES519234A Granted ES519234A0 (es) 1981-04-20 1983-01-25 Un metodo para crear un microorganismo capaz de producir unaproteina predeterminada por medio de expresion.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES511510A Granted ES511510A0 (es) 1981-04-20 1982-04-19 Un metodo para producir una proteina predeterminada por cul tivo de bacterias b subtilis transformadas.

Country Status (12)

Country Link
EP (1) EP0063494B1 (es)
JP (1) JPS58500590A (es)
AT (1) ATE48639T1 (es)
AU (1) AU551529B2 (es)
CA (1) CA1204681A (es)
DE (1) DE3280060D1 (es)
DK (1) DK563082A (es)
ES (2) ES511510A0 (es)
FI (1) FI824238A0 (es)
IL (1) IL65558A0 (es)
NO (1) NO824102L (es)
WO (1) WO1982003633A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO821391L (no) * 1981-04-29 1982-11-01 Biogen Nv Kloningsvektorer, rekombinante dna-molekyler samt bakterieverter transformert med disse
US4559300A (en) * 1983-01-18 1985-12-17 Eli Lilly And Company Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
GR81701B (es) * 1983-01-18 1984-12-12 Lilly Co Eli
US4595660A (en) * 1983-03-02 1986-06-17 University Of Delaware Molecular cloning with bifunctional plasmid vectors in Bacillus subtilis, mutants and substantially stably transformed mutants of Bacillus subtilis, and methods for utilizing the transformed mutants
US4585739A (en) * 1983-03-07 1986-04-29 E. I. Du Pont De Nemours And Company Plasmid for foreign gene expression in B. subtilis
US4663280A (en) * 1983-05-19 1987-05-05 Public Health Research Institute Of The City Of New York Expression and secretion vectors and method of constructing vectors
US5310675A (en) * 1983-06-24 1994-05-10 Genencor, Inc. Procaryotic carbonyl hydrolases
JPS60137291A (ja) * 1983-12-26 1985-07-20 Takeda Chem Ind Ltd 発現ベクター
AU5668486A (en) * 1985-03-29 1986-10-23 Biotechnica International Inc. Secretion vector
IT1186750B (it) * 1985-07-10 1987-12-16 Eniricerche Spa Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0005476B1 (de) * 1978-05-17 1981-12-30 Dr. Karl Thomae GmbH Verfahren zur Herstellung von Humaninterferon
JPS5564799A (en) * 1978-11-07 1980-05-15 Toray Ind Inc Multi-stage concentration and purification of interferon originated from human fibroblast
NL8000127A (nl) * 1979-01-15 1980-07-17 Harvard College Werkwijze voor het vormen van prokaryotische of eukaryotische proteinen alsmede een daarbij toepasbaar gekoppeld gen.
US4311639A (en) * 1980-07-25 1982-01-19 E. I. Du Pont De Nemours And Company Immunogenic interferon peptides

Also Published As

Publication number Publication date
EP0063494B1 (en) 1989-12-13
CA1204681A (en) 1986-05-20
EP0063494A2 (en) 1982-10-27
JPS58500590A (ja) 1983-04-21
ES519234A0 (es) 1984-03-01
NO824102L (no) 1982-12-07
DK563082A (da) 1982-12-20
ATE48639T1 (de) 1989-12-15
DE3280060D1 (de) 1990-01-18
WO1982003633A1 (en) 1982-10-28
FI824238L (fi) 1982-12-09
AU8521382A (en) 1982-11-04
EP0063494A3 (en) 1984-02-22
FI824238A0 (fi) 1982-12-09
ES8308586A1 (es) 1983-09-16
ES511510A0 (es) 1983-09-16
IL65558A0 (en) 1982-07-30
AU551529B2 (en) 1986-05-01

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