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ES2523898T3 - Regiones de escisión KEX2 de proteínas de fusión recombinantes - Google Patents

Regiones de escisión KEX2 de proteínas de fusión recombinantes Download PDF

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Publication number
ES2523898T3
ES2523898T3 ES07861326.2T ES07861326T ES2523898T3 ES 2523898 T3 ES2523898 T3 ES 2523898T3 ES 07861326 T ES07861326 T ES 07861326T ES 2523898 T3 ES2523898 T3 ES 2523898T3
Authority
ES
Spain
Prior art keywords
dna molecule
fusion proteins
recombinant fusion
kex2 cleavage
cleavage regions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES07861326.2T
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English (en)
Inventor
Huaming Wang
Michael Ward
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco US Inc filed Critical Danisco US Inc
Application granted granted Critical
Publication of ES2523898T3 publication Critical patent/ES2523898T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/461—Igs containing Ig-regions, -domains or -residues form different species
    • C07K16/462—Igs containing a variable region (Fv) from one specie and a constant region (Fc) from another
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un constructo de ADN de fusión que codifica un polipéptido de fusión que comprende en unión operable desde el extremo 5' de dicho constructo, un promotor; una primera molécula de ADN que codifica una secuencia señal funcional como una secuencia secretora en una célula fúngica filamentosa; una segunda molécula de ADN que codifica una proteína portadora, donde la proteína portadora es un polipéptido fúngico secretado de manera natural o una porción funcional del mismo; una tercera molécula de ADN que codifica una región KEX2, donde la región KEX2 es VAVEKR; y una cuarta molécula de ADN que codifica una proteína deseada.

Description

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Claims (1)

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ES07861326.2T 2006-07-11 2007-06-21 Regiones de escisión KEX2 de proteínas de fusión recombinantes Active ES2523898T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US484814 2006-07-11
US11/484,814 US20080026376A1 (en) 2006-07-11 2006-07-11 KEX2 cleavage regions of recombinant fusion proteins
PCT/US2007/014476 WO2008048378A2 (en) 2006-07-11 2007-06-21 Kex2 cleavage regions of recombinant fusion proteins

Publications (1)

Publication Number Publication Date
ES2523898T3 true ES2523898T3 (es) 2014-12-02

Family

ID=38986752

Family Applications (3)

Application Number Title Priority Date Filing Date
ES07861326.2T Active ES2523898T3 (es) 2006-07-11 2007-06-21 Regiones de escisión KEX2 de proteínas de fusión recombinantes
ES13151039.8T Active ES2516341T3 (es) 2006-07-11 2007-06-21 Regiones de escisión KEX2 de proteínas de fusión recombinantes
ES13151041.4T Active ES2517524T3 (es) 2006-07-11 2007-06-21 Regiones de escisión KEX2 de proteínas de fusión recombinantes

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES13151039.8T Active ES2516341T3 (es) 2006-07-11 2007-06-21 Regiones de escisión KEX2 de proteínas de fusión recombinantes
ES13151041.4T Active ES2517524T3 (es) 2006-07-11 2007-06-21 Regiones de escisión KEX2 de proteínas de fusión recombinantes

Country Status (10)

Country Link
US (3) US20080026376A1 (es)
EP (3) EP2044202B1 (es)
JP (1) JP5548448B2 (es)
CN (2) CN101490261B (es)
BR (1) BRPI0714432B1 (es)
CA (1) CA2657273C (es)
DK (3) DK2607485T3 (es)
ES (3) ES2523898T3 (es)
SI (3) SI2044202T1 (es)
WO (1) WO2008048378A2 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2225375A1 (en) * 2007-12-21 2010-09-08 Fujifilm Manufacturing Europe B.V. Improvement of the secretion yield of a protein of interest by in vivo proteolytic processing of a multimeric precursor
GB0908770D0 (en) 2009-04-24 2009-07-01 Danisco Method
CA2766009A1 (en) * 2009-06-25 2010-12-29 Danisco A/S Variant lipolytic enzymes with improved expression, functionality and/or activity
EP2494050A4 (en) * 2009-10-30 2013-10-30 Merck Sharp & Dohme GRANULOCYTIC COLONY STIMULATING FACTOR MADE IN GLYCOENGINEERED PICHIA PASTORIS
CN103348003B (zh) 2010-11-24 2016-09-07 诺瓦提斯国际药品有限公司 具有n-乙酰葡糖胺基转移酶活性的融合酶
US8846357B2 (en) 2012-12-20 2014-09-30 E I Du Pont De Nemours And Company Stabilized chlorine dioxide for contamination control in Zymomonas fermentation
RU2675939C2 (ru) * 2013-10-08 2018-12-25 Токай Оптикал Ко., Лтд. Способ оценки для оценки очковой линзы, способ проектирования для проектирования очковой линзы с применением способа оценки и способ вычисления для вычисления характеристик визуального восприятия субъекта при просмотре объекта сквозь линзу
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
WO2020185669A2 (en) * 2019-03-08 2020-09-17 Danisco Us Inc Fusion polypeptides
CN110305223B (zh) * 2019-06-26 2022-05-13 重庆派金生物科技有限公司 重组串联融合蛋白制备目标多肽的方法
JP2023504059A (ja) * 2019-11-27 2023-02-01 レベレイションズ バイオテック プライベート リミティド アスペルギルス・ニガーからのベータ－フルクトフラノシダーゼの製造のための核酸、ベクター、宿主細胞及び方法
PE20221265A1 (es) * 2019-11-27 2022-08-16 Revelations Biotech Pvt Ltd Acidos nucleicos, vectores, celulas huespedes y metodos para la produccion de fructosiltransferasa de aspergillus japonicus
WO2024167695A1 (en) 2023-02-08 2024-08-15 Danisco Us Inc. Compositions and methods for producing heterologous globins in filamentous fungal cells
WO2025007098A1 (en) 2023-06-30 2025-01-02 Danisco Us Inc. Recombinant fungal cells and methods thereof for industrial scale production of lectins

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) * 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
CA1338400C (en) 1983-08-31 1996-06-18 David H. Gelfand Recombinant fungal cellulases
US4797361A (en) * 1983-10-24 1989-01-10 Lehigh University Microorganism and process
EP0625577A1 (en) 1985-08-29 1994-11-23 Genencor International, Inc. Heterologous polypeptides expressed in filamentous fungi, processes for their preparation, and vectors for their preparation
US5364770A (en) * 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
CA1333777C (en) * 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
DK0562003T4 (en) * 1990-12-10 2015-07-13 Danisco Us Inc Improved saccharification of cellulose by cloning and amplification of.-Glucosidase gene from Tricodermareesei
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US6265204B1 (en) * 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
EA005682B1 (ru) * 1998-10-06 2005-04-28 Марк Аарон Эмалфарб Трансформированные грибы, в частности рода chrysosporium, способные к синтезу гетерологичных полипептидов
US6268328B1 (en) * 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
MXPA02001447A (es) * 1999-08-13 2003-07-21 The Victoria University Of Manchester Enzimas de fitasa, acidos nucleicos que codifican para enzimas de fitasa y vectores y celulas hospedadoras que incorporan las mismas.
CA2382609C (en) * 1999-09-09 2012-08-07 Iogen Bio-Products Corporation Expression of proteins in genetically modified fungi
US7795002B2 (en) * 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
WO2003066818A2 (en) * 2002-02-08 2003-08-14 Genencor International, Inc. Secretion, transcription and sporulation genes in bacillus clausii
WO2003089614A2 (en) 2002-04-18 2003-10-30 Genencor International, Inc. Production of functional antibodies in filamentous fungi
ES2340588T3 (es) 2003-05-29 2010-06-07 Genencor Int Genes nuevos de trichoderma.
JP4834554B2 (ja) * 2003-11-06 2011-12-14 ジェネンコー・インターナショナル・インク 糸状菌におけるプロテアーゼ抑制剤及びその変異体の発現
JP5091484B2 (ja) * 2003-11-21 2012-12-05 ジェネンコー・インターナショナル・インク トリコデルマにおける顆粒デンプン加水分解酵素の発現及び顆粒デンプン基質からグルコースシロップを製造する方法
US8097445B2 (en) 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
CA2567570C (en) 2004-05-27 2014-07-08 Genencor International, Inc. Aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis

Also Published As

Publication number Publication date
EP2607485A1 (en) 2013-06-26
CN101490261A (zh) 2009-07-22
JP5548448B2 (ja) 2014-07-16
SI2607486T1 (sl) 2014-12-31
SI2044202T1 (sl) 2014-11-28
DK2607485T3 (da) 2014-09-29
BRPI0714432A2 (pt) 2013-02-13
US20130011877A1 (en) 2013-01-10
US20140024067A1 (en) 2014-01-23
WO2008048378A3 (en) 2008-07-10
US8936917B2 (en) 2015-01-20
CA2657273A1 (en) 2008-04-24
SI2607485T1 (sl) 2014-11-28
CN101490261B (zh) 2013-12-18
US20080026376A1 (en) 2008-01-31
WO2008048378A2 (en) 2008-04-24
DK2607486T3 (da) 2014-10-20
ES2517524T3 (es) 2014-11-03
JP2009542258A (ja) 2009-12-03
EP2607485B1 (en) 2014-07-23
EP2607486A1 (en) 2013-06-26
EP2607486B1 (en) 2014-07-23
EP2044202B1 (en) 2014-08-20
DK2044202T3 (da) 2014-10-20
ES2516341T3 (es) 2014-10-30
CN103805619A (zh) 2014-05-21
EP2044202A2 (en) 2009-04-08
US8518667B2 (en) 2013-08-27
CA2657273C (en) 2018-01-16
BRPI0714432B1 (pt) 2017-11-21

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