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ES2196009T3 - Procedimiento para inmovilizar enzimas en la pared celular de una celula microbiana mediante la produccion de una proteina de fusion. - Google Patents

Procedimiento para inmovilizar enzimas en la pared celular de una celula microbiana mediante la produccion de una proteina de fusion.

Info

Publication number
ES2196009T3
ES2196009T3 ES93915827T ES93915827T ES2196009T3 ES 2196009 T3 ES2196009 T3 ES 2196009T3 ES 93915827 T ES93915827 T ES 93915827T ES 93915827 T ES93915827 T ES 93915827T ES 2196009 T3 ES2196009 T3 ES 2196009T3
Authority
ES
Spain
Prior art keywords
cell wall
pct
enzyme
protein
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93915827T
Other languages
English (en)
Inventor
Franciscus Maria Klis
Maarten Pleun Schreuder
Holger York Toschka
Cornelis Theodorus Verrips
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever NV
Original Assignee
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever NV filed Critical Unilever NV
Application granted granted Critical
Publication of ES2196009T3 publication Critical patent/ES2196009T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/16—Enzymes or microbial cells immobilised on or in a biological cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE PROPORCIONA UN METODO PARA INMOVILIZAR UNA ENZIMA, QUE COMPRENDE LA INMOVILIZACION DE UNA ENZIMA O UNA PARTE FUNCIONAL DE LA MISMA A LA PARED CELULAR DE UNA CELULA MICROBIAL, UTILIZANDO TECNICAS RECOMBINANTES DE DNA. LA ENZIMA SE INMOVILIZA UNIENDOLA AL SEGMENTO C-TERMINAL DE UNA PROTEINA QUE ASEGURA EL ANCLAJE EN LA PARED CELULAR. TAMBIEN PROPORCIONA UN POLINUCLEOTIDO RECOMBINANTE QUE COMPRENDE UN GENESTRUCTURAL QUE CODIFICA UNA PROTEINA ED ENZIMA, UNA PARTE DE UN GEN QUE CODIFICA LA PARTE C-TERMINAL DE UNA PROTEINA CAPAZ DE ANCLARSE EN UNA PARED CELULAR EUCARIOTICA O PROCARIOTICA, ADEMAS DE UNA SECUENCIA DE SEÑAL, Y EN ADICION A UNA PROTEINA QUIMERICA CODIFICADA POR EL POLINUCLEOTIDO RECOMBINANTE Y UN VECTOR Y MICRORGANISMO QUE CONTIENEN EL POLINUCLEOTIDO. EL MICRORGANISMO ES IDONEO PARA LLEVAR A CABO PROCESOS ENZIMATICOS A ESCALA INDUSTRIAL.
ES93915827T 1992-07-08 1993-07-07 Procedimiento para inmovilizar enzimas en la pared celular de una celula microbiana mediante la produccion de una proteina de fusion. Expired - Lifetime ES2196009T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP92202080 1992-07-08
EP92203899 1992-12-14

Publications (1)

Publication Number Publication Date
ES2196009T3 true ES2196009T3 (es) 2003-12-16

Family

ID=26131543

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93915827T Expired - Lifetime ES2196009T3 (es) 1992-07-08 1993-07-07 Procedimiento para inmovilizar enzimas en la pared celular de una celula microbiana mediante la produccion de una proteina de fusion.

Country Status (16)

Country Link
US (1) US6027910A (es)
EP (1) EP0673427B1 (es)
JP (2) JP3681385B2 (es)
KR (1) KR950702634A (es)
AT (1) ATE236257T1 (es)
AU (1) AU685057B2 (es)
CA (1) CA2139670C (es)
DE (1) DE69332829T2 (es)
DK (1) DK0673427T3 (es)
ES (1) ES2196009T3 (es)
FI (1) FI950042A (es)
NZ (1) NZ254108A (es)
OA (1) OA10123A (es)
PT (1) PT673427E (es)
RU (1) RU95105899A (es)
WO (1) WO1994001567A1 (es)

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US20030036092A1 (en) * 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
US5866344A (en) * 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
DK0682710T3 (da) * 1993-02-10 2004-03-01 Unilever Nv Isolationsmetode, ved hvilken der anvendes immobiliserede proteiner med specifikke kapaciteter
BR9404246A (pt) * 1993-02-26 1999-06-15 Sapporo Breweries Genes de aglutinação de levedura e levedura contendo os mesmos
GB9423367D0 (en) * 1994-11-18 1995-01-11 Wellcome Found Enzyme prodrug therapy
US6696251B1 (en) 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6699658B1 (en) 1996-05-31 2004-03-02 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6300065B1 (en) 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6759243B2 (en) 1998-01-20 2004-07-06 Board Of Trustees Of The University Of Illinois High affinity TCR proteins and methods
AU778477B2 (en) 1999-03-25 2004-12-09 Valtion Teknillinen Tutkimuskeskus Process for partitioning of proteins
JP2003284556A (ja) * 1999-11-19 2003-10-07 Toyota Motor Corp 金属捕捉能タンパク質を発現する遺伝子
ATE428733T1 (de) 2000-03-14 2009-05-15 Unilever Nv Variabele domänen der schweren kette eines antikörpers gegen menschliche ernährungslipasen und deren verwendungen
DE10016279A1 (de) * 2000-04-03 2001-10-04 Henkel Kgaa Enzymatisches Färbemittel
DE60135215D1 (de) * 2000-05-01 2008-09-18 Daiichi Sankyo Co Ltd Verfahren zum screening von auf zellwände wirkenden arzneistoffen
US7063738B2 (en) * 2000-05-15 2006-06-20 Schlumberger Technology Corporation Permeable cements
KR100888946B1 (ko) 2000-07-07 2009-03-17 에자이 알앤드디 매니지먼트 가부시키가이샤 진균의 세포벽 합성유전자
US20020165149A1 (en) * 2000-12-08 2002-11-07 Kranz David M. Mutated class II major histocompatibility proteins
DE60230128D1 (de) * 2001-04-19 2009-01-15 Kansai Chem Eng Protein, das sich an die zelloberflächenschichten bindet, und dessen verwendung
DE60232071D1 (de) 2001-06-11 2009-06-04 Applied Nanosystems Bv Verfahren zur bindung von acma-typ protein anker fusionen an mikroorganismen zellwandmaterialien
CA2462113C (en) * 2001-10-01 2013-01-29 Dyax Corp. Multi-chain eukaryotic display vectors and uses thereof
WO2003035898A1 (fr) 2001-10-22 2003-05-01 Daiichi Pharmaceutical Co., Ltd. Methode de criblage d'un medicament agissant sur la paroi cellulaire
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
JP5236189B2 (ja) 2003-12-05 2013-07-17 チルドレンズ ホスピタル メディカル センター オリゴ糖組成物および感染症の治療における該組成物の使用
WO2007005612A2 (en) * 2005-07-01 2007-01-11 Medimmune, Inc. An integrated approach for generating multidomain protein therapeutics
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US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US20080138663A1 (en) * 2006-12-12 2008-06-12 Canon Kabushiki Kaisha Microbial electrode and fuel cell and sensor using the same
JP2010517577A (ja) * 2007-02-09 2010-05-27 メディミューン，エルエルシー 酵母細胞原形質膜による抗体ライブラリー提示
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Also Published As

Publication number Publication date
NZ254108A (en) 1995-12-21
EP0673427A1 (en) 1995-09-27
AU4565393A (en) 1994-01-31
US6027910A (en) 2000-02-22
PT673427E (pt) 2003-08-29
WO1994001567A1 (en) 1994-01-20
JPH07508652A (ja) 1995-09-28
KR950702634A (ko) 1995-07-29
CA2139670C (en) 2005-09-27
ATE236257T1 (de) 2003-04-15
FI950042A0 (fi) 1995-01-04
DE69332829D1 (de) 2003-05-08
EP0673427B1 (en) 2003-04-02
FI950042A (fi) 1995-01-24
DK0673427T3 (da) 2003-07-21
CA2139670A1 (en) 1994-01-20
AU685057B2 (en) 1998-01-15
JP2004254700A (ja) 2004-09-16
OA10123A (en) 1996-12-18
JP3681385B2 (ja) 2005-08-10
RU95105899A (ru) 1997-03-20
DE69332829T2 (de) 2004-01-22

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